keyword
MENU ▼
Read by QxMD icon Read
search

Modeling tuberculosis

keyword
https://www.readbyqxmd.com/read/28436741/deep-learning-at-chest-radiography-automated-classification-of-pulmonary-tuberculosis-by-using-convolutional-neural-networks
#1
Paras Lakhani, Baskaran Sundaram
Purpose To evaluate the efficacy of deep convolutional neural networks (DCNNs) for detecting tuberculosis (TB) on chest radiographs. Materials and Methods Four deidentified HIPAA-compliant datasets were used in this study that were exempted from review by the institutional review board, which consisted of 1007 posteroanterior chest radiographs. The datasets were split into training (68.0%), validation (17.1%), and test (14.9%). Two different DCNNs, AlexNet and GoogLeNet, were used to classify the images as having manifestations of pulmonary TB or as healthy...
April 24, 2017: Radiology
https://www.readbyqxmd.com/read/28436453/functional-thermodynamics-structural-and-biological-studies-of-in-silico-identified-inhibitors-of-mycobacterium-tuberculosis-enoyl-acp-coa-reductase-enzyme
#2
Leonardo K B Martinelli, Mariane Rotta, Anne D Villela, Valnês S Rodrigues-Junior, Bruno L Abbadi, Rogério V Trindade, Guilherme O Petersen, Giuliano M Danesi, Laura R Nery, Ivani Pauli, Maria M Campos, Carla D Bonan, Osmar Norberto de Souza, Luiz A Basso, Diogenes S Santos
Novel chemotherapeutics agents are needed to kill Mycobacterium tuberculosis, the main causative agent of tuberculosis (TB). The M. tuberculosis 2-trans-enoyl-ACP(CoA) reductase enzyme (MtInhA) is the druggable bona fide target of isoniazid. New chemotypes were previously identified by two in silico approaches as potential ligands to MtInhA. The inhibition mode was determined by steady-state kinetics for seven compounds that inhibited MtInhA activity. Dissociation constant values at different temperatures were determined by protein fluorescence spectroscopy...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28435012/il-10-down-regulates-the-expression-of-survival-associated-gene-hspx-of-mycobacterium-tuberculosis-in-murine-macrophage
#3
Babban Jee, Pawan Sharma, Kiran Katoch, Beenu Joshi, Sudhir Kumar Awasthi
Mycobacterium tuberculosis (MTB) adopts a special survival strategy to overcome the killing mechanism(s) of host immune system. Amongst the many known factors, small heat shock protein 16.3 (sHSP16.3) of MTB encoded by gene hspX has been reported to be critical for the survival of MTB. In the present study, the effect of recombinant murine interferon-gamma (rmIFN-γ) and recombinant murine interleukin-10 (rmIL-10) on the expression of gene hspX of MTB in murine macrophage RAW264.7 has been investigated. By real-time RT-PCR, it was observed that three increasing concentrations (5, 25 and 50ng/ml) of rmIFN-γ significantly up-regulated the expression of hspX whereas similar concentrations of rmIL-10 (5, 25 and 50ng/ml) significantly down-regulated the hspX expression...
April 20, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28434937/defining-a-research-agenda-to-address-the-converging-epidemics-of-tuberculosis-and-diabetes-part-2-underlying-biological-mechanisms
#4
REVIEW
Katharina Ronacher, Reinout van Crevel, Julia Critchley, Andrew A Bremer, Larry S Schlesinger, Anil Kapur, Randall Basaraba, Hardy Kornfeld, Blanca I Restrepo
There is growing interest in the re-emerging interaction between type 2 diabetes (DM) and tuberculosis (TB), but the underlying biological mechanisms are poorly understood despite their possible implications in clinical management. Experts in epidemiological, public health, basic science and clinical studies recently convened and identified research priorities for elucidating the underlying mechanisms for the co-ocurrence of TB and DM. We identified gaps in current knowlege of altered immunity in DM patients during TB, where most studies suggest an under-performing innate immunity, but exaggerated adaptive immunity to Mycobacterium tuberculosis...
April 19, 2017: Chest
https://www.readbyqxmd.com/read/28427367/determinants-of-delayed-care-seeking-for-tb-suggestive-symptoms-in-seru-district-oromiya-region-ethiopia-a-community-based-unmatched-case-control-study
#5
Robel Yirgu, Firaol Lemessa, Selamawit Hirpa, Abraham Alemayehu, Eveline Klinkenberg
BACKGROUND: Early tuberculosis (TB) case finding and adequate chemotherapy are essential for interrupting disease transmission and preventing complications due to delayed care seeking. This study was undertaken in order to provide insights into the magnitude and determinants of patient delay. METHODS: The study was conducted in rural Seru district, employing a population based unmatched case-control study design. The WHO standardized TB screening tool was used to identify presumptive TB cases among the district population ages > 15 years...
April 20, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28426991/clinical-usefulness-of-18-f-fdg-pet-ct-for-initial-staging-and-assessment-of-treatment-efficacy-in-patients-with-lymph-node-tuberculosis
#6
Nicolas Lefebvre, Xavier Argemi, Nicolas Meyer, Joy Mootien, Nawal Douiri, Stefania Sferrazza-Mandala, Frédéric Schramm, Noëlle Weingertner, Daniel Christmann, Yves Hansmann, Alessio Imperiale
INTRODUCTION: Few studies have evaluated the promising role of (18)F-fluoro-2-deoxy-D-glucose positron emission tomography (PET) and PET/computed tomography FDG PET/CT in evaluating and monitoring treatment response in patients with lymph node tuberculosis (LNTB). The aim of this clinical investigation was to assess the clinical usefulness of FDG PET/CT for initial tuberculosis staging and to determine the prognostic value of the decrease of (18)F-FDG uptake during antibiotic treatment in LNTB patients...
April 8, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/28425484/mycobacterium-tuberculosis-resistance-prediction-and-lineage-classification-from-genome-sequencing-comparison-of-automated-analysis-tools
#7
Viola Schleusener, Claudio U Köser, Patrick Beckert, Stefan Niemann, Silke Feuerriegel
Whole-genome sequencing (WGS) has the potential to accelerate drug-susceptibility testing (DST) to design appropriate regimens for drug-resistant tuberculosis (TB). Several recently developed automated software tools promise to standardize the analysis and interpretation of WGS data. We assessed five tools (CASTB, KvarQ, Mykrobe Predictor TB, PhyResSE, and TBProfiler) with regards to DST and phylogenetic lineage classification, which we compared with phenotypic DST, Sanger sequencing, and traditional typing results for a collection of 91 strains...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28425478/data-intensive-genome-level-analysis-for-identifying-novel-non-toxic-drug-targets-for-multi-drug-resistant-mycobacterium-tuberculosis
#8
Divneet Kaur, Rintu Kutum, Debasis Dash, Samir K Brahmachari
We report the construction of a novel Systems Biology based virtual drug discovery model for the prediction of non-toxic metabolic targets in Mycobacterium tuberculosis (Mtb). This is based on a data-intensive genome level analysis and the principle of conservation of the evolutionarily important genes. In the 1623 sequenced Mtb strains, 890 metabolic genes identified through a systems approach in Mtb were evaluated for non-synonymous mutations. The 33 genes showed none or one variation in the entire 1623 strains, including 1084 Russian MDR strains...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28424703/immune-responses-to-bacillus-calmette-gu%C3%A3-rin-vaccination-why-do-they-fail-to-protect-against-mycobacterium-tuberculosis
#9
REVIEW
Juan I Moliva, Joanne Turner, Jordi B Torrelles
Mycobacterium tuberculosis (M.tb), the causative agent of tuberculosis (TB), is the current leading cause of death due to a single infectious organism. Although curable, the broad emergence of multi-, extensive-, extreme-, and total-drug resistant strains of M.tb has hindered eradication efforts of this pathogen. Furthermore, computational models predict a quarter of the world's population is infected with M.tb in a latent state, effectively serving as the largest reservoir for any human pathogen with the ability to cause significant morbidity and mortality...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28424682/interactions-between-type-1-interferons-and-the-th17-response-in-tuberculosis-lessons-learned-from-autoimmune-diseases
#10
REVIEW
Bas C Mourik, Erik Lubberts, Jurriaan E M de Steenwinkel, Tom H M Ottenhoff, Pieter J M Leenen
The classical paradigm of tuberculosis (TB) immunity, with a central protective role for Th1 responses and IFN-γ-stimulated cellular responses, has been challenged by unsatisfactory results of vaccine strategies aimed at enhancing Th1 immunity. Moreover, preclinical TB models have shown that increasing IFN-γ responses in the lungs is more damaging to the host than to the pathogen. Type 1 interferon signaling and altered Th17 responses have also been associated with active TB, but their functional roles in TB pathogenesis remain to be established...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28424495/significance-of-genetic-polymorphisms-in-long-non-coding-rna-ac079767-4-in-tuberculosis-susceptibility-and-clinical-phenotype-in-western-chinese-han-population
#11
Zhenzhen Zhao, Mei Zhang, Jun Ying, Xuejiao Hu, Jingya Zhang, Yanhong Zhou, Yi Zhou, Xingbo Song, Binwu Ying
Recent studies have implicated long non-coding RNA, AC079767.4, as a highly susceptible gene in tuberculosis. The aim of the study was to preliminarily explore the possible association of single nucleotide polymorphisms (SNPs) in AC079767.4 gene with clinical phenotypes and TB susceptibility in Western Chinese Han population. The improved multiplex ligation detection reaction (iMLDR) method was employed to genotype 4 SNPs in AC079767.4 in 554 tuberculosis patients and 561 healthy individuals. In subgroup analysis, only the C allele for rs12477677 was associated with the decreased susceptibility to pulmonary TB with a p-value of 0...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28422240/zwitterionic-pyrrolidene-phosphonates-inhibitors-of-the-glycoside-hydrolase-like-phosphorylase-streptomyces-coelicolor-glgei-v279s
#12
Sri Kumar Veleti, Cecile Petit, Donald R Ronning, Steven J Sucheck
We synthesized and evaluated new zwitterionic inhibitors against glycoside hydrolase-like phosphorylase Streptomyces coelicolor (Sco) GlgEI-V279S which plays a role in α-glucan biosynthesis. Sco GlgEI-V279S serves as a model enzyme for validated anti-tuberculosis (TB) target Mycobacterium tuberculosis (Mtb) GlgE. Pyrrolidine inhibitors 5 and 6 were designed based on transition state considerations and incorporate a phosphonate on the pyrrolidine moiety to expand the interaction network between the inhibitor and the enzyme active site...
April 19, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28419153/biochemical-and-structural-investigations-on-phosphoribosylpyrophosphate-synthetase-from-mycobacterium-smegmatis
#13
Stefano Donini, Silvia Garavaglia, Davide M Ferraris, Riccardo Miggiano, Shigetarou Mori, Keigo Shibayama, Menico Rizzi
Mycobacterium smegmatis represents one model for studying the biology of its pathogenic relative Mycobacterium tuberculosis. The structural characterization of a M. tuberculosis ortholog protein can serve as a valid tool for the development of molecules active against the M. tuberculosis target. In this context, we report the biochemical and structural characterization of M. smegmatis phosphoribosylpyrophosphate synthetase (PrsA), the ortholog of M. tuberculosis PrsA, the unique enzyme responsible for the synthesis of phosphoribosylpyrophosphate (PRPP)...
2017: PloS One
https://www.readbyqxmd.com/read/28419146/household-crowding-as-a-potential-mediator-of-socioeconomic-determinants-of-tuberculosis-incidence-in-brazil
#14
Daniele Maria Pelissari, Fredi Alexander Diaz-Quijano
Although many studies have identified social conditions associated with tuberculosis, contextual and individual factors have rarely been analysed simultaneously. Consequently, we aimed to identify contextual and individual factors associated with tuberculosis incidence in general population in Brazil in 2010. We also assessed whether household crowding mediates the association between socioeconomic determinants and tuberculosis incidence. Individual data of tuberculosis cases were obtained from 5,565 municipalities in Brazil in 2010 (last year of national census), and merged with contextual variables...
2017: PloS One
https://www.readbyqxmd.com/read/28414774/mycobacterium-marinum-antagonistically-induces-an-autophagic-response-while-repressing-the-autophagic-flux-in-a-torc1-and-esx-1-dependent-manner
#15
Elena Cardenal-Muñoz, Sonia Arafah, Ana Teresa López-Jiménez, Sébastien Kicka, Alexandra Falaise, Frauke Bach, Olivier Schaad, Jason S King, Monica Hagedorn, Thierry Soldati
Autophagy is a eukaryotic catabolic process also participating in cell-autonomous defence. Infected host cells generate double-membrane autophagosomes that mature in autolysosomes to engulf, kill and digest cytoplasmic pathogens. However, several bacteria subvert autophagy and benefit from its machinery and functions. Monitoring infection stages by genetics, pharmacology and microscopy, we demonstrate that the ESX-1 secretion system of Mycobacterium marinum, a close relative to M. tuberculosis, upregulates the transcription of autophagy genes, and stimulates autophagosome formation and recruitment to the mycobacteria-containing vacuole (MCV) in the host model organism Dictyostelium...
April 17, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28414012/reasons-for-optimism-in-the-search-for-new-vaccines-for-tuberculosis
#16
T G Evans
In the development of vaccines for tuberculosis (TB), the combination of the will, funding, scientific rigor, new tools, refined animal models and improved clinical trial designs are all converging at an opportune moment. The lack of optimism that has surrounded the likelihood for finding novel TB vaccines has resulted from a lack of correlates of vaccine-induced protection, a lack of tool candidate vaccines to probe the immunologic space, which may be needed, and the negative result of one recent trial. A vaccine for TB that can be delivered at a reasonable cost to the marketplace will have greater impact on the incidence of new cases of TB than any intervention in world history...
April 17, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28413722/performance-of-interferon-%C3%AE-release-assay-in-the-diagnosis-of-tuberculous-lymphadenitis-a-meta-analysis
#17
Qianqian Liu, Wenzhang Li, Yunfeng Chen, Xinmiao Du, Chengdi Wang, Binmiao Liang, Yin Tang, Yulin Feng, Chuanmin Tao, Jian-Qing He
BACKGROUND: The diagnostic values of interferon-gamma release assays (IGRA) in tuberculosis (TB) vary a lot with different site of infections, with especially higher sensitivities in chronic forms of TB such as tuberculosis of the lymph node. We conducted a meta-analysis to comprehensively evaluate the overall accuracy of diagnostic IGRA for tuberculous lymphadenitis. METHODS: Pubmed, Web of Science, EMBASE, Wanfang and CNKI databases up to February 17, 2017 were searched to identify published studies...
2017: PeerJ
https://www.readbyqxmd.com/read/28412150/epidemiological-features-of-and-changes-in-incidence-of-infectious-diseases-in-china-in-the-first-decade-after-the-sars-outbreak-an-observational-trend-study
#18
Shigui Yang, Jie Wu, Cheng Ding, Yuanxia Cui, Yuqing Zhou, Yiping Li, Min Deng, Chencheng Wang, Kaijin Xu, Jingjing Ren, Bing Ruan, Lanjuan Li
BACKGROUND: The model of infectious disease prevention and control changed significantly in China after the outbreak in 2003 of severe acute respiratory syndrome (SARS), but trends and epidemiological features of infectious diseases are rarely studied. In this study, we aimed to assess specific incidence and mortality trends of 45 notifiable infectious diseases from 2004 to 2013 in China and to investigate the overall effectiveness of current prevention and control strategies. METHODS: Incidence and mortality data for 45 notifiable infectious diseases were extracted from a WChinese public health science data centre from 2004 to 2013, which covers 31 provinces in mainland China...
April 12, 2017: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/28411471/qsar-docking-studies-of-1-3-thiazinan-3-yl-isonicotinamide-derivatives-for-antitubercular-activity
#19
Trupti S Chitre, Kalyani D Asgaonkar, Shital M Patil, Shiva Kumar, Vijay M Khedkar, Dinesh R Garud
The enzyme - enoyl acyl carrier protein reductase (enoyl ACP reductase) is a validated target for antitubercular activity. Inhibition of this enzyme interferes with mycolic acid synthesis which is crucial for Mycobacterium tuberculosis cell growth. In the present work 2D and 3D quantitative structure activity relationship (QSAR) studies were carried out on a series of thiazinan-Isoniazid pharmacophore to design newer analogues. For 2D QSAR, the best statistical model was generated using SA-MLR method (r(2)=0...
March 30, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/28410979/post-migration-follow-up-of-migrants-identified-to-be-at-increased-risk-of-developing-tuberculosis-at-pre-migration-screening-a-systematic-review-and-meta-analysis
#20
Isaac H Y Chan, Nishta Kaushik, Claudia C Dobler
BACKGROUND: Post-migration follow-up of migrants considered at increased risk of developing tuberculosis based on pre-migration screening abnormalities (high-risk migrants) is implemented in several low-incidence countries. We aimed to determine the rate of tuberculosis in this population to inform cross-border tuberculosis control policies. METHODS: We searched MEDLINE and Embase (since inception to Jan 12, 2017) for studies evaluating post-migration follow-up of high-risk migrants...
April 11, 2017: Lancet Infectious Diseases
keyword
keyword
115925
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"