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E S Von Zuben, A G Oliveira, M Chorilli, M V Scarpa
A reversed-phase liquid chromatography (RP-LC) method was successfully developed and validated for the determination of methotrexate in nanostructured liquid crystalline systems composed by polyether functional siloxane and silicone polyether copolymer. The LC method was performed on RP C18-ODS column, Agilent Zorbax® (4.6 x 250 mm, 5 μm), maintained at room temperature, with a mobile phase constituted by a mixture of 50 mM ammonium acetate buffer (pH 6.0) and methanol (77:23,v/v) with a flow rate of 1.0 mL/min, using ultraviolet detection at 313 nm...
March 5, 2018: Die Pharmazie
Fantin Girard, Conrad Kavalec, Farida Cheriet
Optic disc (OD) appearance in fundus images is one of the clinical indicators considered in the assessment of retinal diseases such as glaucoma. The cup-to-disc ratio (CDR) is the most common clinical measurement used to characterize glaucoma. However, the CDR only evaluates the relative sizes of the cup and the OD via their diameters. We propose to construct an atlas-based shape descriptor (ASD) to statistically characterize the geometric deformations of the OD shape and of the blood vessels' configuration inside the OD region...
January 2018: Journal of Medical Imaging
Jing Zhan, Ya Ding, Benyan Zou, Hai Liao, Wenqi Jiang, Su Li
BACKGROUND AND OBJECTIVE: Puquitinib mesylate (XC-302) is a new multiple-target anticancer inhibitor, which directly suppresses the activity of phosphatidylinositol 3-kinase (PI3K). This study was aimed to develop a sensitive and specific liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI MS/MS) method for the quantification and pharmacokinetic investigation of plasma puquitinib in cancer patients. METHODS: The analytes of human plasma were prepared by liquid-liquid extraction using methyl-t-butyl ether (MTBE)...
March 8, 2018: European Journal of Drug Metabolism and Pharmacokinetics
Satoko Mishima-Kimura, Kosei Yonemitsu, Yuki Ohtsu, Ako Sasao, Hiroshi Tsutsumi, Shota Furukawa, Yoko Nishitani
We report a forensic autopsy case of an elderly man who ingested unknown amount of germicidal disinfectant containing 50% benzalkonium chloride (BZK). He survived for 18 days after BZK ingestion and then died because of pneumonia. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used to detect three BZK compounds (C12 -BZK, C14 -BZK and C16 -BZK) in the blood. Extraction of BZK was carried out according to a modified QuEChERS method. Chromatographic separation was achieved on an ODS column and detection was performed in selected reaction monitoring mode...
March 2, 2018: Legal Medicine
V Piloni, M Bergamasco, G Melara, P Garavello
BACKGROUND: The aim of the present study was to assess the relationship between symptoms of obstructed defecation and findings on magnetic resonance (MR) defecography in males with obstructed defecation syndrome (ODS). METHODS: Thirty-six males with ODS who underwent MR defecography at our institution between March 2013 and February 2016 were asked in a telephone interview about their symptoms and subsequent treatment, either medical or surgical. Patients were divided into 2 groups, one with anismus (Group 1) and one with prolapse without anismus (Group 2)...
March 6, 2018: Techniques in Coloproctology
Henki Rotinsulu, Hiroyuki Yamazaki, Shino Sugai, Natsuki Iwakura, Defny S Wewengkang, Deiske A Sumilat, Michio Namikoshi
Cladosporamide A (1), a new protein tyrosine phosphatase (PTP) 1B inhibitor, was isolated together with a known prenylated flavanone derivative (2) from the culture broth of an Indonesian marine sponge-derived Cladosporium sp. TPU1507 by solvent extraction, ODS column chromatography, and preparative HPLC (ODS). The structure of 1 was elucidated based on 1D and 2D NMR data. Compound 1 modestly inhibited PTP1B and T-cell PTP (TCPTP) activities with IC50 values of 48 and 54 μM, respectively. The inhibitory activity of 2 against PTP1B (IC50  = 11 μM) was approximately 2-fold stronger than that against TCPTP (IC50  = 27 μM)...
March 5, 2018: Journal of Natural Medicines
Yeong-Geun Lee, Seon-A Jang, Kyeong-Hwa Seo, Jung Eun Gwag, Hyoung-Geun Kim, Jung-Hwan Ko, So-Ae Ji, Se-Chan Kang, Dae Young Lee, Nam-In Baek
Six lignans including two new lignans were obtained as the principal components of the Forsythia koreana flowers via silica gel (SiO2 ), octadecyl SiO2 (ODS) as well Sephadex LH-20 column chromatography. In addition to two new lignans, named koreanaside A ((7R,8S,7'R,8'S)-5'-hydroxy-3,3'-dimethoxy-7,7'-diepoxylignan 4-O-β-D-glucopyranoside) and koreanaside B ((7R,8S,7'S,8'R)-9,5',7'-trihydroxy-3,3'-dimethoxy-7,9'-epoxylignan 4-O-β-D-glucopyranoside), four known lignans were identified to be (+)-phylligenin, (-)-epipinoresinol, pinoresinol, and tinosposide A...
March 1, 2018: Chemistry & Biodiversity
Gui-Jie Zhang, Lu-Mei Dai, Bin Zhang, Yong-Li Zhang, Heng-Shan Wang, Dong Liang
Ten compounds were isolated from the 95% aqueous EtOH extract of Clerodendrum bungei by a combination of various chromatographic techniques including column chromatography over silica gel, Sephadex LH-20, MCI, ODS, and semi-preparative HPLC. Their structures were elucidated as 11,12,16S-trihydroxy-7-oxo-17(15→16),18(4→3)-diabeo-abieta-3,8,11,13-tetraen-18-oic acid (1), 12S*,13R*-dihydroxy-9-oxo-octadeca-10(E)-enoic acid (2), clerodenoside A (3), trichotomoside (4), glycosmisic acid (5), 4'-O-methylscutellarein (6), neroplomacrol (7), butylitaconic acid (8), hexylitaconic acid (9), p-hydroxybenzonic acid (10) by their physicochemical properties and spectroscopic data...
December 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Iwona Jóźwik, Agata Strojny-Nędza, Marcin Chmielewski, Katarzyna Pietrzak, Łukasz Kurpaska, Szymon Nosewicz
The performance of the present-day scanning electron microscopy (SEM) extends far beyond delivering electronic images of the surface topography. Oxide dispersion strengthened (ODS) steel is on of the most promising materials for the future nuclear fusion reactor because of its good radiation resistance, and higher operation temperature up to 750°C. The microstructure of ODS should not exceed tens of nm, therefore there is a strong need in a fast and reliable technique for their characterization. In this work, the results of low-kV SEM characterization of nanoprecipitates formed in the ODS matrix are presented...
February 21, 2018: Microscopy Research and Technique
Nicola Lamberti, Sofia Straudi, Efisio Lissia, Lorenza Cavazzini, Sergio Buja, Roberto Manfredini, Nino Basaglia, Fabio Manfredini
BACKGROUND: Peripheral arterial disease (PAD) is a common cardiovascular pathology affecting mobility in elderly. Osteoarticular diseases (ODs), responsible for functional limitations and confounding leg symptoms, may interfere with exercise therapy. This study evaluates the feasibility and effectiveness of a structured home-based exercise programme on rehabilitative outcomes in a cohort of elderly PAD patients with and without coexisting ODs. PATIENTS AND METHODS: Patients were enrolled from 2002 to 2016 in an exercise programme prescribed and controlled at the hospital and based on two daily 10-minute home walking sessions below the self-selected speed...
February 21, 2018: VASA. Zeitschrift Für Gefässkrankheiten
Celestin Missikpode, Corinne Peek-Asa, Tracy Young, Cara Hamann
INTRODUCTION: Emergency vehicles, such as police, ambulances, and fire vehicles, need to arrive at the scene of emergencies as quickly as possible, and thus they often travel in emergency mode - using their lights and sirens and often bypassing traffic signals. We examined whether travelling in emergency mode increased crash risk among police, ambulance and fire vehicles. METHODS: We conducted a quasi-induced exposure analysis using data from the Iowa Crash Database for the period of 2005 through 2013...
February 10, 2018: Accident; Analysis and Prevention
M Brunner, H Roth, K Günther, R Grützmann, K E Matzel
PURPOSE: Ventral mesh rectopexy (VMR) is an established, minimally invasive, nerve-sparing procedure for the treatment of various symptomatic morphological changes in the posterior pelvic compartment. We present the short-term functional outcome and patient satisfaction after laparoscopic and robotic VMR with biological mesh. METHODS: We analyzed data from 123 patients who underwent laparoscopic ventral mesh rectopexy (LVMR) or robotic ventral mesh rectopexy (RVMR) from August 2012 to January 2017...
February 13, 2018: International Journal of Colorectal Disease
Ehab F Elkady, Marwa A Fouad, Bushra M Jaadan
A new validated bio-analytical LC-MS/MS method was developed for the simultaneous extraction and determination of four proton pump inhibitors: esomeprazole, lansoprazole, pantoprazole and rabeprazole in human plasma using escitalopram as an internal standard. The proteins in plasma samples were precipitated using acetonitrile for the extraction of analytes which is a simple economic method. The separation was accomplished using a mobile phase composed of 10 mM ammonium formate: acetonitrile: methanol (20:40:40% v/v) at a flow rate of 0...
February 15, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Binan Zhu, Yuting Lu, Leilin Chen, Binbin Yu, Yuexin Liu, Min Song, Taijun Hang
A sensitive and selective method was developed for the separation and characterization of related substances (RSs) in EVT-401 by hyphenated LC-MS techniques. Complete separation of the RSs was achieved with an Inertsil ODS-SP column (250 mm×4.6 mm, 5 µm) by linear gradient elution using a mobile phase consisting of 0.2% formic acid solution, methanol and acetonitrile. EVT-401 was found to be susceptible to acid, alkaline and oxidative stresses, while relatively stable under photolytic and thermal dry stress conditions...
August 2017: Journal of Pharmaceutical Analysis
Thomas Humphries, Rajiv Singh
OBJECTIVES: Olfactory disturbance (OD) is common after traumatic brain injury (TBI). Screening for OD can be performed by several different methods. While odour identification tools are considered more accurate, they are time consuming. The predominant method in clinical practice remains the use of a single odour. This study aimed to compare a brief single-odour identification tool (BSOIT) with a more detailed 12-odour assessment tool. MATERIALS/METHODS: One hundred seventy consecutive patients with TBI had their olfaction assessed using BSOIT and a 12-item tool at a single time point...
February 2, 2018: Brain Injury: [BI]
Hongjiang Chen, Jianan Liu, Mingchao Cui, Jianwei Chen, Xiang Li, Yong Chen
A rapid and simple analytical method was established for the determination of four amides (N-p-trans-coumaroyltyramine, aristolactam AⅡ, sauristolactam and aristolactam BⅡ) in Saururus chinensis by matrix solid phase dispersion (MSPD) and high-performance liquid chromatography-diode array detector (HPLC-DAD). In the optimized MSPD, 0.2 g S. chinensis powder was blended with 0.4 g silica gel, and 5 mL methanol was selected as elution solvent. The MSPD extraction achieved higher extraction recovery of four amides, and required less sample, solvent and preparation time, comparing with the conventional methods (Soxhlet and ultrasonic extraction)...
January 2018: Journal of Food and Drug Analysis
Ali Naci Yildiz, Tahir Metin Piskin, Mehmet Erdem Alaguney, Ozlem Kar Kurt, Ahmet Ozlu, Mustafa Kemal Başaralı
OBJECTIVES: The incidence of ODs is below expectations. The approaches of family physicians is very important. In this study, we aimed to evaluate attitudes and behaviors of family physicians about ODs. METHODS: The questionnaire was first sent to family physicians via e-mail. Of 3663 responders, 3090 replies were included to study. RESULTS: 44.1% of them stated they obtained detailed occupational history. In logistic regression analysis, physicians who obtained detailed occupational history, discussed with occupational physician about their patients' health, had education about ODs and those who stated that they wanted to receive training in ODs were more likely to refer their patient...
February 1, 2018: Archives of Environmental & Occupational Health
Lei Zhang, Zhengkang Duan, Hongwen Zhu, Ke Yin
An analytical method was developed for the determination of iminodiacetic acid (IDA) and glycine (Gly), the dehydrogenation products of diethanolamine, by high performance liquid chromatography (HPLC) coupled with pre-column derivatization using p-toluenesulfonyl chloride (PTSC) as the derivatization reagent. IDA and Gly reacted with PTSC in the alkaline environment (pH 11) under 45℃ for 15 min. Then the derivatization products were analyzed by HPLC-MS. The separation was carried out on a high performance liquid chromatograph equipped with an ultraviolet detector...
November 8, 2017: Se Pu, Chinese Journal of Chromatography
Tōru Kimura, Ayano Tajima, Yuki Inahashi, Masato Iwatsuki, Hiroaki Kasai, Takayuki Mokudai, Yoshimi Niwano, Kazuro Shiomi, Yōko Takahashi, Satoshi Ōmura, Takuji Nakashima
A new antibiotic, designated mumiamicin, was isolated from the cultured broth of the rare actinomycete strain, Mumia sp. YSP-2-79, by Diaion HP-20, silica gel and ODS column chromatography, followed by HPLC purification. The chemical structure of mumiamicin was elucidated as a new furan fatty acid by nuclear magnetic resonance and mass spectrometry. Mumiamicin showed antimicrobial activity and antioxidative activity.
January 25, 2018: Journal of General and Applied Microbiology
Michele Schiano di Visconte, Francesco Nicolì, Arianna Pasquali, Gabriele Bellio
PURPOSE: The long-term efficacy of stapled transanal rectal resection (STARR) for surgical management of obstructed defecation syndrome (ODS) has not been evaluated. Therefore, we investigated the long-term efficacy (>10 years) of STARR for treatment of ODS related to rectocele or rectal intussusception and the factors that predict treatment outcome. METHODS: This study was a retrospective cohort analysis conducted on prospectively collected data. Seventy-four consecutive patients who underwent STARR for ODS between January 2005 and December 2006 in two Italian hospitals were included...
January 24, 2018: Colorectal Disease: the Official Journal of the Association of Coloproctology of Great Britain and Ireland
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