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https://www.readbyqxmd.com/read/29316517/functional-metagenomics-reveals-a-novel-carbapenem-hydrolyzing-mobile-beta-lactamase-from-indian-river-sediments-contaminated-with-antibiotic-production-waste
#1
Nachiket P Marathe, Anders Janzon, Stathis D Kotsakis, Carl-Fredrik Flach, Mohammad Razavi, Fanny Berglund, Erik Kristiansson, D G Joakim Larsson
Evolution has provided environmental bacteria with a plethora of genes that give resistance to antibiotic compounds. Under anthropogenic selection pressures, some of these genes are believed to be recruited over time into pathogens by horizontal gene transfer. River sediment polluted with fluoroquinolones and other drugs discharged from bulk drug production in India constitute an environment with unprecedented, long-term antibiotic selection pressures. It is therefore plausible that previously unknown resistance genes have evolved and/or are promoted here...
January 6, 2018: Environment International
https://www.readbyqxmd.com/read/29294946/safe-from-harm-youth-self-report-of-physical-assault-in-child-welfare-placements
#2
Jesse J Helton, Tatiana Gochez-Kerr
Although most child welfare placements receive a rigorous assessment and ongoing safety monitoring, it is still unclear which is safest in terms of physical abuse. Our goals for this study were to assess the relative risk of physical assault for different child welfare placements in a national sample of youth and examine that risk within placement types for children with various levels of behavioral problems. Unlike previous studies of assault prevalence, we constrained our analysis to youth self-reports of assaults within a current placement to increase validity...
October 1, 2017: Journal of Interpersonal Violence
https://www.readbyqxmd.com/read/29227447/heat-shock-proteins-in-human-perilymph-implications-for-cochlear-implantation
#3
Heike Schmitt, Ariane Roemer, Carsten Zeilinger, Rolf Salcher, Martin Durisin, Hinrich Staecker, Thomas Lenarz, Athanasia Warnecke
OBJECTIVE: Biomarkers reflecting the etiology and pathophysiology of inner ear diseases are limited. Evaluation of proteins in the perilymph may improve our understanding of inner ear disease. Heat shock proteins (HSP) belong to a superfamily of stress proteins and promote refolding of denatured proteins. The aim of the study was to analyze HSP in human perilymph and to identify possible correlation with audiological and etiologic data. METHODS: Sampling of the perilymph was performed during cochlear implantation and vestibular schwannoma removal...
January 2018: Otology & Neurotology
https://www.readbyqxmd.com/read/29202338/cranial-trauma-in-handgun-executions-experimental-data-using-polyurethane-proxies
#4
Seth C Taylor, Elena F Kranioti
Gun violence is a global phenomenon with regional variation in frequency and severity. Handguns are often used in violent deaths such as suicides and homicides. Hence, ballistic trauma is a critical subject of forensic investigations. Trauma patterns are fundamental evidence for the reconstruction of the incident and for the determination of the manner of death. This study investigated the differences in trauma patterns with a series of experiments using six different calibers (.22 LR, .38 Special, .380 ACP, 9×19mm, ...
November 27, 2017: Forensic Science International
https://www.readbyqxmd.com/read/29161377/transmission-and-persistence-of-incf-conjugative-plasmids-in-the-gut-microbiota-of-full-term-infants
#5
Anuradha Ravi, Lorena Valdés-Varela, Miguel Gueimonde, Knut Rudi
Conjugative plasmids represent major reservoirs for horizontal transmission of antibiotic resistance (AR) and virulence genes. Our knowledge about the ecology and persistence of these plasmids in the gut microbiota remains limited. The IncF plasmids are the most widespread in clinical samples and in healthy humans. Therefore, the main aim is to study their ecology and association with the developing gut microbiota. Using a longitudinal (2, 10, 30 and 90 days) cohort of full-term infants, we investigated the transmission and persistence of IncFIA and IncFIB plasmids...
November 17, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29099622/electroencephalographic-assessment-of-concussive-non-penetrative-captive-bolt-stunning-of-turkeys
#6
T J Gibson, C B Rebelo, T A Gowers, N M Chancellor
1. The aim of this study was to evaluate the electroencephalographic (EEG) and behavioural responses of turkeys stunned with three different concussive non-penetrative captive-bolt guns prior to slaughter. 2. Thirty-one slaughter weight ex-breeding female turkeys (29 weeks of age; mean body weight 13.32 ± SD 0.65 kg) were stunned with the Cash Poultry Killer (CPK) (n=10), Turkey Euthanasia Device (TED) (n=10) and Zephyr EXL (n=11). 3. Mean peak kinetic energy was highest for the CPK compared to the TED and Zephyr EXL (75...
November 3, 2017: British Poultry Science
https://www.readbyqxmd.com/read/29082452/the-death-of-ben-hall
#7
Roger W Byard, Damian McDonald
Ben Hall was a nineteenth-century Australian bushranger (outlaw) who was shot and killed by colonial police on May 5 1865. Popular belief is that Hall was shot while sleeping in his camp bedding. This contrasts with the official police version of Hall being shot while attempting to escape by running away. To evaluate this divergence of opinion a study of the gun belt allegedly worn by Hall at the time of his fatal shooting was undertaken. This revealed a nineteenth-century belt with a defect corresponding to an oblique bullet hole...
October 30, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/29078894/the-utility-of-presacral-drainage-in-penetrating-rectal-injuries-in-adult-and-pediatric-patients
#8
Kate B Savoie, Thomas M Beazley, Brent Cleveland, Sina Khaneki, Troy A Markel, Peter M Hammer, Stephanie Savage, Regan F Williams
BACKGROUND: With changing weaponry associated with injuries in civilian trauma, there is no clinical census on the utility of presacral drainage (PSD) in penetrating rectal injuries (PRIs), particularly in pediatric patients. METHODS: Patients with PRI from July 2004-June 2014 treated at two free-standing children's hospitals and two adult level 1 trauma centers were compared by age (pediatric patients ≤16 years) and PSD. A stratified analysis was performed based on age...
November 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/29058106/line-of-fire-what-happened-at-the-wantabadgery-seige
#9
Roger W Byard, Adam Ford, Tony Raymond, Jeremy J Sofonia, Owen Kaluza, David G Barnes
A gunfight between police and a gang of men led by the self-styled "Captain Moonlite", a.k.a. George Scott, occurred on 16th November 1879 at a farmhouse near Wantabadgery Station in the colony of New South Wales. The skirmish resulted in the deaths of two bushrangers and one police officer. As a result, Captain Moonlite and Thomas Rogan were hung in Sydney's Darlinghurst Gaol on 20 January 1880 for the murder of Constable Edward Webb-Bowen. Culpability for firing the fatal shot, however, has remained a source of controversy...
October 23, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/29051082/magnetic-capture-of-polydopamine-encapsulated-hela-cells-for-the-analysis-of-cell-surface-proteins
#10
Yiying Liu, Guoquan Yan, Mingxia Gao, Xiangmin Zhang
A novel method to characterize cell surface proteins and complexes has been developed. Polydopamine (PDA)-encapsulated Hela cells were prepared for plasma membrane proteome research. Since the PDA protection, the encapsulated cells could be maintained for more than two weeks. Amino groups functionalized magnetic nanoparticles were also used for cell capture by the reaction with the PDA coatings. Plasma membrane fragments were isolated and enriched with assistance of an external magnetic field after disruption of the coated cells by ultrasonic treatment...
October 16, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29038950/firing-a-loaded-gun-during-sleep-in-an-elderly-man-with-a-perfect-storm-of-risk-factors-including-severe-obstructive-sleep-apnea
#11
Francesca Ingravallo, Carlos H Schenck, Ernesto D'Aloja, Monica Puligheddu
PURPOSE: We report a case of firing a loaded gun during sleep in a geriatric patient with undiagnosed major sleep disorders and multiple risk factors for sleep violence. Polysomnographic findings, diagnostic challenges, and forensic implications in this unprecedented geriatric case are discussed. METHODS: A 75-year-old employed man, married for 32 years, presented to a sleep center reporting to having fired a shot in his bedroom during sleep while his wife was away, without memory of hearing the gunshot...
October 16, 2017: Sleep & Breathing, Schlaf & Atmung
https://www.readbyqxmd.com/read/29038580/a-novel-library-of-arylketones-as-potential-inhibitors-of-%C3%AE-glucosidase-their-design-synthesis-in-vitro-and-in-vivo-studies
#12
Tania Luthra, Rahul Agarwal, Mamidala Estari, Uma Adepally, Subhabrata Sen
α-glucosidase is an essential enzyme located at the brush border of intestines. It is an important therapeutic target for type II diabetes. Herein we have designed a library of novel α-arylketones as inhibitors of α-glucosidase (yeast origin) via scaffold hopping and bioisosteric modification of known inhibitors of α-glucosidase. The design was validated through molecular docking that revealed strong binding interactions of the newly designed compounds against α-glucosidase. A library comprising of 15 compounds was synthesized in a combinatorial fashion, where the advanced amide intermediates were accessed through "shot gun" synthesis...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29017638/the-difference-between-living-and-dying-victim-characteristics-and-motive-among-nonfatal-shootings-and-gun-homicides
#13
Natalie Kroovand Hipple, Lauren A Magee
Using both official and unofficial data sources, researchers examined nonfatal (n = 617) and fatal shooting (n = 159) victim characteristics over an 18-month period in Indianapolis. This research revealed that the typical shooting victim was male, non-White, almost 29 years old, had been arrested prior to inclusion in this study, and had been shot more than once. Interestingly, this research supports the notion that nonfatal shooting and homicide victims are different, especially as they relate to victim age, gunshot wound severity, and shooting motive...
October 10, 2017: Violence and Victims
https://www.readbyqxmd.com/read/28983838/how-race-affects-evidence-accumulation-during-the-decision-to-shoot
#14
Timothy J Pleskac, Joseph Cesario, David J Johnson
The biasing role of stereotypes is a central theme in social cognition research. For example, to understand the role of race in police officers' decisions to shoot, participants have been shown images of Black and White males and instructed to shoot only if the target is holding a gun. Findings show that Black targets are shot more frequently and more quickly than Whites. The decision to shoot has typically been modeled and understood as a signal detection process in which a sample of information is compared against a criterion, with the criterion set for Black targets being lower...
October 5, 2017: Psychonomic Bulletin & Review
https://www.readbyqxmd.com/read/28900110/enhancement-of-environmental-hazard-degradation-in-the-presence-of-lignin-a-proteomics-study
#15
Su Sun, Shangxian Xie, Yanbing Cheng, Hongbo Yu, Honglu Zhao, Muzi Li, Xiaotong Li, Xiaoyu Zhang, Joshua S Yuan, Susie Y Dai
Proteomics studies of fungal systems have progressed dramatically based on the availability of more fungal genome sequences in recent years. Different proteomics strategies have been applied toward characterization of fungal proteome and revealed important gene functions and proteome dynamics. Presented here is the application of shot-gun proteomic technology to study the bio-remediation of environmental hazards by white-rot fungus. Lignin, a naturally abundant component of the plant biomass, is discovered to promote the degradation of Azo dye by white-rot fungus Irpex lacteus CD2 in the lignin/dye/fungus system...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729959/shot-gun-proteome-and-transcriptome-mapping-of-the-jujube-floral-organ-and-identification-of-a-pollen-specific-s-locus-f-box-gene
#16
Ruihong Chen, Guoliang Chen, Jian Huang
The flower is a plant reproductive organ that forms part of the fruit produced as the flowering season ends. While the number and identity of proteins expressed in a jujube (Ziziphus jujuba Mill.) flower is currently unknown, integrative proteomic and transcriptomic analyses provide a systematic strategy of characterizing the floral biology of plants. We conducted a shotgun proteomic analysis on jujube flowers by using a filter-aided sample preparation tryptic digestion, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
2017: PeerJ
https://www.readbyqxmd.com/read/28717964/wound-morphology-in-contact-shots-from-blank-cartridge-handguns-a-study-on-composite-models
#17
Rebecca Pircher, Markus Große Perdekamp, Annette Thierauf-Emberger, Lena Kramer, Stefan Pollak, Dorothee Geisenberger
It is a well-known fact that blank cartridge guns can cause penetrating and even fatal injuries when discharged in contact or at very close ranges. In these cases, the gas jet perforates the skin leaving an entrance wound similar to that from a conventional gun. In order to investigate the wound morphology in contact shots from blank firearms, test shots were fired at composite models of pig skin and gelatin blocks using three different calibre 9-mm blank cartridge handguns (two pistols and one revolver) and two types of ammunition...
July 17, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28710235/renal-fistulae-different-aetiologies-similar-management
#18
Ved Bhaskar, Rahul Janak Sinha, Bimalesh Purkait, Vishwajeet Singh
Iatrogenic renocolic fistulae, although have been described in literature, is a rare clinical complication. Recently its incidence is on rise due to advent of minimally invasive surgery and percutaneous surgery of kidney. It has been reported after percutaneous nephrolithotomy but its incidence after percutaneous nephrostomy is quite uncommon and rarely reported. Though spontaneous renocutaneous fistula has been reported, acquired renocutaneous fistula is very uncommon and fistula after gun shot injury has not been reported to the best of our knowledge...
July 14, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28706004/feedback-microtubule-control-and-microtubule-actin-cross-talk-in-arabidopsis-revealed-by-integrative-proteomic-and-cell-biology-analysis-of-katanin-1-mutants
#19
Tomáš Takáč, Olga Šamajová, Tibor Pechan, Ivan Luptovčiak, Jozef Šamaj
Microtubule organization and dynamics are critical for key developmental processes such as cell division, elongation, and morphogenesis. Microtubule severing is an essential regulator of microtubules and is exclusively executed by KATANIN 1 in Arabidopsis In this study, we comparatively studied the proteome-wide effects in two KATANIN 1 mutants. Thus, shotgun proteomic analysis of roots and aerial parts of single nucleotide mutant fra2 and T-DNA insertion mutant ktn1-2 was carried out. We have detected 42 proteins differentially abundant in both fra2 and ktn1-2 KATANIN 1 dysfunction altered the abundance of proteins involved in development, metabolism, and stress responses...
September 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28684708/porcine-epidemic-diarrhea-in-europe-in-detail-analyses-of-disease-dynamics-and-molecular-epidemiology
#20
Dennis Hanke, Anne Pohlmann, Carola Sauter-Louis, Dirk Höper, Julia Stadler, Mathias Ritzmann, Adi Steinrigl, Bernd-Andreas Schwarz, Valerij Akimkin, Robert Fux, Sandra Blome, Martin Beer
Porcine epidemic diarrhea (PED) is an acute and highly contagious enteric disease of swine caused by the eponymous virus (PEDV) which belongs to the genus Alphacoronavirus within the Coronaviridae virus family. Following the disastrous outbreaks in Asia and the United States, PEDV has been detected also in Europe. In order to better understand the overall situation, the molecular epidemiology, and factors that might influence the most variable disease impact; 40 samples from swine feces were collected from different PED outbreaks in Germany and other European countries and sequenced by shot-gun next-generation sequencing...
July 6, 2017: Viruses
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