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https://www.readbyqxmd.com/read/28538943/low-serum-chromium-is-rare-in-patients-that-underwent-endoscopic-gastrostomy-for-long-term-enteral-feeding
#1
Carla Adriana Santos, Jorge Fonseca, Elisabete Carolino, António Sousa Guerreiro
BACKGROUND: Patients that underwent Percutaneous Endoscopic Gastrostomy (PEG) present with protein-energy malnutrition. Trace elements are required in small quantities and Chromium (Cr) displays a major role in the metabolism. OBJECTIVE: This study aims to evaluate Cr levels and its relationship with serum proteins, BMI and underlying diseases during the first 3 months of PEG feeding. METHODS: Prospective observational study during 3-months, when PEG was performed (T0), after 4 (T1), and 12 weeks (T3)...
May 18, 2017: Arquivos de Gastroenterologia
https://www.readbyqxmd.com/read/28538738/solid-phase-synthesis-cyclization-and-site-specific-functionalization-of-aziridine-containing-tetrapeptides
#2
Benjamin K W Chung, Christopher J White, Andrei K Yudin
Cyclic tetrapeptides comprise a potent and selective class of molecules with a wide range of biological activities, including the phytotoxic activity of tentoxin and the histone deacetylase (HDAC) inhibitory effects of chlamydocin. The incorporation of a functional aziridine group within cyclic peptides enables their conformational control and allows for late-stage and site-selective functionalization of these molecules, thereby creating the potential for covalent protein labeling. This protocol describes the solid-phase synthesis, cyclization, and site-specific structural modification of aziridine-containing tetrapeptides...
June 2017: Nature Protocols
https://www.readbyqxmd.com/read/28538165/the-complexity-in-herpesvirus-entry
#3
REVIEW
Karthik Sathiyamoorthy, Jia Chen, Richard Longnecker, Theodore S Jardetzky
Enveloped viruses have evolved diverse transmembrane proteins and protein complexes to enable host cell entry by regulating and activating membrane fusion in a target cell-specific manner. In general terms, the entry process requires a receptor binding step, an activation step and a membrane fusion step, which can be encoded within a single viral protein or distributed among multiple viral proteins. HIV and influenza virus, for example, encode all of these functions in a single trimeric glycoprotein, HIV env or influenza virus hemagglutinin (HA)...
May 21, 2017: Current Opinion in Virology
https://www.readbyqxmd.com/read/28538148/active-site-pka-determination-for-photoactive-yellow-protein-rationalizes-slow-ground-state-recovery
#4
Nur Alia Oktaviani, Trijntje J Pool, Yuichi Yoshimura, Hironari Kamikubo, Ruud M Scheek, Mikio Kataoka, Frans A A Mulder
The ability to avoid blue-light radiation is crucial for bacteria to survive. In Halorhodospira halophila, the putative receptor for this response is known as photoactive yellow protein (PYP). Its response to blue light is mediated by changes in the optical properties of the chromophore para-coumaric acid (pCA) in the protein active site. PYP displays photocycle kinetics with a strong pH dependence for ground-state recovery, which has remained enigmatic. To resolve this problem, a comprehensive pKa determination of the active-site residues of PYP is required...
May 23, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28538145/nmr-analysis-of-amide-hydrogen-exchange-rates-in-a-pentapeptide-repeat-protein-from-a-%C3%A2-thaliana
#5
Shenyuan Xu, Shuisong Ni, Michael A Kennedy
At2g44920 from Arabidopsis thaliana is a pentapeptide-repeat protein (PRP) composed of 25 repeats capped by N- and C-terminal α-helices. PRP structures are dominated by four-sided right-handed β-helices typically consisting of mixtures of type II and type IV β-turns. PRPs adopt repeated five-residue (Rfr) folds with an Rfr consensus sequence (STAV)(D/N)(L/F)(S/T/R)(X). Unlike other PRPs, At2g44920 consists exclusively of type II β-turns. At2g44920 is predicted to be located in the thylakoid lumen although its biochemical function remains unknown...
May 23, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28538092/discriminating-residue-substitutions-in-a-single-protein-molecule-using-a-sub-nanopore
#6
Zhuxin Dong, Eamonn Kennedy, Mohammad Hokmabadi, Gregory Timp
It is now possible to create, in a thin inorganic membrane, a single, sub-nanometer-diameter pore, i.e. a sub-nanopore, about the size of an amino acid residue. To explore the prospects for sequencing protein with it, measurements of the force and current were performed as two denatured histones, which differed by four amino acid residue substitutions, were impelled systematically through the sub-nanopore one-at-a-time using an atomic force microscope. The force measurements revealed that once the denatured protein, stabilized by sodium dodecyl sulfate (SDS), translocated through the sub-nanopore, a disproportionately large force was required to pull it back...
May 24, 2017: ACS Nano
https://www.readbyqxmd.com/read/28537976/interest-of-urinary-timp-2-%C3%A3-igfbp-7-for-predicting-the-occurrence-of-acute-kidney-injury-after-cardiac-surgery-a-gray-zone-approach
#7
Toufic Finge, Sebastien Bertran, Claire Roger, Damien Candela, Bruno Pereira, Christopher Scott, Laurent Muller, Benjamin Louart, Jean-Yves Lefrant
BACKGROUND: This study assessed the ability of 3-hour postoperative urinary tissue inhibitor of metalloproteinases-2 × insulin-like growth factor binding protein-7 ([TIMP-2] × [IGFBP-7]) to predict postoperative acute kidney injury (AKI) in patients undergoing cardiopulmonary bypass during cardiac surgery. METHODS: Patients undergoing cardiac surgery with cardiopulmonary bypass were eligible for this study. Patients with initial chronic renal insufficiency requiring renal replacement therapy, patients <18 years of age, and parturients were not included...
May 19, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28537922/cooperative-but-distinct-early-co-signaling-events-originate-from-erbb2-and-erbb1-receptors-upon-trastuzumab-treatment-in-breast-cancer-cells
#8
Paola Bagnato, Alessia Castagnino, Katia Cortese, Maria Bono, Silvia Grasso, Grazia Bellese, Tiziana Daniele, Richard Lundmark, Paola Defilippi, Patrizio Castagnola, Carlo Tacchetti
ERBB2 receptor belongs to the ERBB tyrosine kinase receptor family. At variance to the other family members, ERBB2 is a constitutively active orphan receptor. Upon ligand binding and activation, ERBB receptors form homo- or hetero-dimers with the other family members, including ERBB2, promoting an intracellular signaling cascade. ERBB2 is the preferred dimerization partner and ERBB2 heterodimers signaling is stronger and longer acting compared to heterodimers between other ERBB members. The specific contribution of ERBB2 in heterodimer signaling is still undefined...
May 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28537903/amyloid-precursor-like-protein-1-promotes-jnk-mediated-cell-migration-in-drosophila
#9
Xingjun Wang, Ying Sun, Shilong Han, Chenxi Wu, Yeqing Ma, Yu Zhao, Yingyao Shao, Yujun Chen, Lingzhi Kong, Wenzhe Li, Fan Zhang, Lei Xue
The amyloid precursor like protein-1 (APLP1) is a member of the amyloid precursor protein (APP) family in mammals. While many studies have been focused on the pathologic role of APP in Alzheimer's disease, the physiological functions of APLP1 have remained largely elusive. Here we report that ectopic expression of APLP1 in Drosophila induces cell migration, which is suppressed by the loss of JNK signaling and enhanced by the gain of JNK signaling. APLP1 activates JNK signaling through phosphorylation of JNK, which up-regulates the expression of matrix metalloproteinase MMP1 required for basement membranes degradation and promotes actin remodeling essential for cell migration...
May 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28537900/wisteria-floribunda-agglutinin-positive-human-mac-2-binding-protein-predicts-liver-cancer-development-in-chronic-hepatitis-b-patients-under-antiviral-treatment
#10
Ka-Shing Cheung, Wai-Kay Seto, Danny Ka-Ho Wong, Lung-Yi Mak, Ching-Lung Lai, Man-Fung Yuen
AIM: The risk factors for hepatocellular carcinoma (HCC) development in chronic hepatitis B (CHB) patients with undetectable serum HBV DNA under nucleos(t)ide analogue (NA) therapy are not well defined. We aimed to examine the relationship between Wisteria floribunda agglutinin-positive human Mac-2 binding protein (WFA+-M2BP) and HCC development in these patients. RESULTS: There was a significant difference in the median levels of pre-treatment WFA+-M2BP between the HCC and control groups (0...
May 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28537849/efficacy-safety-and-preparation-of-standardized-parenteral-nutrition-regimens-three-chamber-bags-vs-compounded-monobags-a-prospective-multicenter-randomized-single-blind-clinical-trial
#11
Yu Jianchun, Wu Guohao, Tang Yun, Ye Yingjiang, Zhang Zhongtao
BACKGROUND: Parenteral nutrition (PN) covering the need for carbohydrates, amino acids, and lipids can either be compounded from single nutrients or purchased as an industrially manufactured ready-to-use regimen. This study compares a commercially available 3-chamber bag (study group) with a conventionally compounded monobag regarding nutrition efficacy, safety, and regimen preparation time. MATERIALS AND METHODS: This prospective, randomized, single-blind study was conducted at 5 Chinese hospitals from October 2010-October 2011...
May 1, 2017: Nutrition in Clinical Practice
https://www.readbyqxmd.com/read/28537798/neonatal-morbidity-count-is-associated-with-a-reduced-likelihood-of-achieving-recommendations-for-protein-lipid-and-energy-in-very-low-birth-weight-infants-a-prospective-cohort-study
#12
Dawn V Y Ng, Sharon Unger, Michelle Asbury, Alex Kiss, Rosine Bishara, Nicole Bando, Chris Tomlinson, Sharyn Gibbins, Deborah L O'Connor
BACKGROUND: Serious morbidity may elevate nutrient requirements and affect adherence to feeding guidelines for very low birth weight (VLBW) infants. An understanding of factors affecting nutrient intakes of VLBW infants will facilitate development of strategies to improve nutrient provision. Our aim was to examine the impact of neonatal morbidity count on achieving recommended nutrient intakes in VLBW infants. METHODS: VLBW infants enrolled in the Donor Milk for Improved Neurodevelopmental Outcomes trial (ISRCTN35317141, n = 363) were included...
May 1, 2017: JPEN. Journal of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28537725/the-color-of-cation-%C3%AF-interactions-subtleties-of-amine-tryptophan-interaction-energetics-allow-for-radical-like-visible-absorbance-and-fluorescence
#13
Laura J Juszczak, Azaria S Eisenberg
Several peptides and a protein with an inter- or intramolecular cation-π interaction between tryptophan (Trp) and an amine cation are shown to absorb and fluoresce in the visible region of the spectrum. Titration of indole with sodium hydroxide or ammonium hydroxide yields an increasing visible fluorescence as well. Visible absorption and multi-peaked fluorescence excitation spectra correlate with experimental absorption spectra and the vibrational modes of calculated absorption spectra for the neutral Trp radical...
May 24, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28535664/characterization-of-alphax-i-domain-binding-to-receptors-for-advanced-glycation-end-products-rage
#14
Dolgorsuren Buyannemekh, Sang-Uk Nham
The b2 integrins are cell surface transmembrane proteins regulating leukocyte functions, such as adhesion and migration. Two members of b2 integrin, aMb2 and aXb2, share the leukocyte distribution profile and integrin aXb2 is involved in antigen presentation in dendritic cells and transendothelial migration of monocytes and macrophages to atherosclerotic lesions. Receptor for advanced glycation end products (RAGE), a member of cell adhesion molecules, plays an important role in chronic inflammation and atherosclerosis...
May 24, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28535307/intergenerational-impact-of-paternal-lifetime-exposures-to-both-folic-acid-deficiency-and-supplementation-on-reproductive-outcomes-and-imprinted-gene-methylation
#15
Lundi Ly, Donovan Chan, Mahmoud Aarabi, Mylène Landry, Nathalie A Behan, Amanda J MacFarlane, Jacquetta Trasler
STUDY QUESTION: Do paternal exposures to folic acid deficient (FD), and/or folic acid supplemented (FS) diets, throughout germ cell development adversely affect male germ cells and consequently offspring health outcomes? SUMMARY ANSWER: Male mice exposed over their lifetimes to both FD and FS diets showed decreased sperm counts and altered imprinted gene methylation with evidence of transmission of adverse effects to the offspring, including increased postnatal-preweaning mortality and variability in imprinted gene methylation...
May 23, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28535146/role-of-spatial-inhomogenity-in-gpcr-dimerisation-predicted-by-receptor-association-diffusion-models
#16
Sneha A Deshpande, Aiswarya B Pawar, Anish Dighe, Chaitanya A Athale, Durba Sengupta
G protein-coupled receptor (GPCR) association is an emerging paradigm with far reaching implications in the regulation of signalling pathways and therapeutic interventions. Recent super resolution microscopy studies have revealed that receptor dimer steady state exhibits sub-second dynamics. In particular the GPCRs, muscarinic acetylcholine receptor M1 (M1MR) and formyl peptide receptor (FPR), have been demonstrated to exhibit a fast association/dissociation kinetics, independent of ligand binding. In this work, we have developed a spatial kinetic Monte Carlo model to investigate receptor homo-dimerisation at a single receptor resolution...
May 23, 2017: Physical Biology
https://www.readbyqxmd.com/read/28535040/cancer-cell-discrimination-using-host-guest-doubled-arrays
#17
Ngoc D B Le, Gulen Yesilbag Tonga, Rubul Mout, Sung Tae Kim, Marcos E Wille, Subinoy Rana, Karen A Dunphy, D Joseph Jerry, Mahdieh Yazdani, Rajesh Ramanathan, Caren M Rotello, Vincent M Rotello
We report a nanosensor that uses cell lysates to rapidly profile the tumorigenicity of cancer cells. This sensing platform uses host-guest interactions between cucurbit[7]uril (CB[7]) and the cationic headgroup of a gold nanoparticle (AuNP) to non-covalently modify the binding of three fluorescent proteins of a multichannel sensor in situ. This approach doubles the number of output channels to six, providing single-well identification of cell lysates with 100 % accuracy. Significantly, this classification could be extended beyond the training set, determining the invasiveness of novel cell lines...
May 23, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28535012/neokestose-suppresses-the-growth-of-human-melanoma-a2058-cells-via-inhibition-of-the-nuclear-factor%C3%A2-%C3%AE%C2%BAb-signaling-pathway
#18
Jiann-Shing Wu, Jan-Yi Chang, C Will Chen, Ming-Tse Lin, Dey-Chyi Sheu, Shun-Mei Lee
Neokestose has superior prebiotic effects compared with the commercial fructooligosaccharides (FOS). In addition, the branched structure of neokestose, a type of neo‑FOS, confers improved chemical stability compared with conventional FOS; therefore, the investigation of the branched structure by the present study may be of high biomedical value. The present study aimed to determine whether neokestose may suppress growth of the A2058 melanoma cell line. The cells were initially treated with neokestose; subsequently, in vitro cytotoxicity was assessed using MTT, and cell cycle progression and apoptosis were detected using flow cytometry...
May 17, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28534998/proliferation%C3%A2-inhibiting-pathways-in-liver-regeneration-review
#19
Menggang Liu, Ping Chen
Liver regeneration, an orchestrated process, is the primary compensatory mechanism following liver injury caused by various factors. The process of liver regeneration consists of three stages: Initiation, proliferation and termination. Proliferation‑promoting factors, which stimulate the recovery of mitosis in quiescent hepatocytes, are essential in the initiation and proliferation steps of liver regeneration. Proliferation‑promoting factors act as the 'motor' of liver regeneration, whereas proliferation inhibitors arrest cell proliferation when the remnant liver reaches a suitable size...
May 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28534988/p21-activated-protein-kinase-1-induces-the-invasion-of-gastric-cancer-cells-through-c-jun-nh2-terminal-kinase-mediated-activation-of-matrix-metalloproteinase-2
#20
Liang-Hui Li, Guo-Yang Wu, Yi-Zhuo Lu, Xue-Hua Chen, Bing-Ya Liu, Min-Hua Zheng, Jian-Chun Cai
Gastric cancer (GC) is one of the most common malignancies worldwide. The prognosis of GC is poor, mostly due to widespread metastasis. p21-activated kinase 1 (Pak1), the best characterized member of an evolutionarily conserved family of serine/threonine kinases, plays an important role in the regulation of cell morphogenesis, motility, mitosis and angiogenesis. By qRT-PCR and Gelatin zymograph assay, we demonstrated in the present study that stable overexpression of Pak1 induced matrix metalloproteinase (MMP)-2 mRNA expression and activity in the human MKN45 GC cell line...
May 16, 2017: Oncology Reports
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