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https://www.readbyqxmd.com/read/28110426/influenza-a-virus-upregulates-prpf8-gene-expression-to-increase-virus-production
#1
Chee-Hing Yang, Hui-Chun Li, Yu-Ling Shiu, Tzu-Shan Ku, Chia-Wen Wang, Yi-Shuan Tu, Hung-Ling Chen, Cheng-Hao Wu, Shih-Yen Lo
A ddRT-PCR analysis was performed to detect cellular genes that are differentially expressed after influenza A virus (H1N1) infection of A549 cells. After ddRT-PCR, eight DNA fragments were identified. PRPF8, one of the cellular genes that were upregulated after virus infection, was further analyzed since it has previously been identified as a cellular factor required for influenza virus replication. The upregulation of PRPF8 gene expression after viral infection was confirmed using real-time RT-PCR for mRNA detection and Western blot analysis for protein detection...
January 21, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28110414/expression-of-the-citrus-cstip2-1-gene-improves-tobacco-plant-growth-antioxidant-capacity-and-physiological-adaptation-under-stress-conditions
#2
Cristina P S Martins, Diana M Neves, Luciana C Cidade, Amanda F S Mendes, Delmira C Silva, Alex-Alan F Almeida, Mauricio A Coelho-Filho, Abelmon S Gesteira, Walter S Soares-Filho, Marcio G C Costa
Overexpression of the citrus CsTIP2;1 improves plant growth and tolerance to salt and drought stresses by enhancing cell expansion, H 2 O 2 detoxification and stomatal conductance. Tonoplast intrinsic proteins (TIPs) are a subfamily of aquaporins, belonging to the major intrinsic protein family. In a previous study, we have shown that a citrus TIP isoform, CsTIP2;1, is highly expressed in leaves and also transcriptionally regulated in leaves and roots by salt and drought stresses and infection by 'Candidatus Liberibacter asiaticus', the causal agent of the Huanglongbing disease, suggesting its involvement in the regulation of the flow of water and nutrients required during both normal growth and stress conditions...
January 21, 2017: Planta
https://www.readbyqxmd.com/read/28110333/requirements-for-septal-localization-and-chromosome-segregation-activity-of-the-dna-translocase-sfta-from-bacillus-subtilis
#3
Nina El Najjar, Christine Kaimer, Thomas Rösch, Peter L Graumann
Bacillus subtilis possesses 2 DNA translocases that affect late stages of chromosome segregation: SftA separates nonsegregated DNA prior to septum closure, while SpoIIIE rescues septum-entrapped DNA. We provide evidence that SftA is associated with the division machinery via a stretch of 47 amino acids within its N-terminus, suggesting that SftA is recruited by protein-protein interactions with a component of the division machinery. SftA was also recruited to mid-cell in the absence of its first 20 amino acids, which are proposed to contain a membrane-binding motif...
January 21, 2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28110251/gold-nanomaterials-for-the-selective-capturing-and-sers-diagnosis-of-toxins-in-aqueous-and-biological-fluids
#4
Waleed A Hassanain, Emad L Izake, Michael S Schmidt, Godwin A Ayoko
A highly sensitive nanosensing method for the combined selective capture and SERS detection of Microcystin-LR (MC-LR) in blood plasma has been developed. The new method utilizes gold coated magnetic nanoparticles that are functionalized with anti MC-LR antibody Fab' fragments for the selective capture of MC-LR from aqueous media and blood plasma. Using an oriented immobilization approach, the Fab' fragments are covalently attached to gold surface to form a monolayer with high capture efficiency towards the toxin...
January 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28110187/insecticidal-activities-of-histone-deacetylase-inhibitors-against-a-dipteran-parasite-of-sheep-lucilia-cuprina
#5
Neil H Bagnall, Barney M Hines, Andrew J Lucke, Praveer K Gupta, Robert C Reid, David P Fairlie, Andrew C Kotze
Histone deacetylase inhibitors (HDACi) are being investigated for the control of various human parasites. Here we investigate their potential as insecticides for the control of a major ecto-parasite of sheep, the Australian sheep blowfly, Lucilia cuprina. We assessed the ability of HDACi from various chemical classes to inhibit the development of blowfly larvae in vitro, and to inhibit HDAC activity in nuclear protein extracts prepared from blowfly eggs. The HDACi prodrug romidepsin, a cyclic depsipeptide that forms a thiolate, was the most potent inhibitor of larval growth, with equivalent or greater potency than three commercial blowfly insecticides...
January 10, 2017: International Journal for Parasitology, Drugs and Drug Resistance
https://www.readbyqxmd.com/read/28110167/chiral-separation-of-new-sulfonamide-derivatives-and-evaluation-of-their-enantioselective-affinity-for-human-carbonic-anhydrase-ii-by-microscale-thermophoresis-and-surface-plasmon-resonance
#6
Tiphaine Rogez-Florent, Catherine Foulon, Anne-Sophie Drucbert, Nadège Schifano, Perrine Six, Stéphanie Devassine, Patrick Depreux, Pierre-Marie Danzé, Laurence Goossens, Cécile Danel, Jean-François Goossens
The aim of this study was to develop a method combining chiral separation and biophysical techniques to evaluate the enantioselective affinity of original sulfonamide derivatives towards their therapeutic target, the human carbonic anhydrase II (hACII). The first step consisted in the preparation of the enantiomers by chromatographic separation. The performances of HPLC and Supercritical Fluid Chromatography (SFC) were studied at the analytical scale by optimization of various experimental conditions using adsorbed polysaccharide chiral stationary phases (amylose AD-H and cellulose OD-H)...
January 9, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28109881/production-of-aba-responses-requires-both-the-nuclear-and-cytoplasmic-functional-involvement-of-pyr1
#7
EunJoo Park, Tae-Houn Kim
Abscisic acid (ABA) enhances stress tolerant responses in plants against unfavorable environmental conditions. In Arabidopsis, ABA promotes interactions between PYR/PYL/RCARs and PP2C, thereby allowing SnRK2s to phosphorylate downstream components required for the regulation of gene expression or for gating ion channels. Because PYR1 is known to localize to nucleus and cytoplasm it is a question whether nuclear or cytoplasmic PYR1 confer different functions to the ABA signaling pathway, as has been previously shown for regulatory proteins...
January 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28109812/chitosan-as-a-vehicle-for-growth-factor-delivery-various-preparations-and-their-applications-in-bone-tissue-regeneration
#8
REVIEW
Jayachandran Venkatesan, Sukumaran Anil, Se-Kwon Kim, Min Suk Shim
The replacement of conventional autografts and allografts by bone fragments constructed from alternate materials, cells, and molecules (growth factors, drugs, etc.) is an exciting prospect in the field of bone tissue engineering. Bone morphogenetic protein-2 (BMP-2) is a growth factor that has been extensively studied from this point of view. This review analyzes the relevance of chitosan and its derivatives and composites with various materials such as ceramics, heparin, silica, stem cells, titanium implants, etc...
January 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28109756/membrane-cholesterol-oxidation-in-live-cells-enhances-the-function-of-serotonin1a-receptors
#9
Md Jafurulla, Aswan Nalli, Amitabha Chattopadhyay
The serotonin1A (5-HT1A) receptor is an important neurotransmitter receptor that belongs to the G protein-coupled receptor (GPCR) family. It is implicated in a variety of cognitive and behavioral functions and serves as an important drug target for neuropsychiatric disorders such as anxiety and depression. Previous work from our laboratory has demonstrated that membrane cholesterol plays an important role in the function of the serotonin1A receptor. Our earlier results highlighted several structural features of cholesterol essential for receptor function...
January 18, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/28109603/comparison-between-direct-and-indirect-methods-for-exploiting-fourier-transform-spectral-information-in-estimation-of-breeding-values-for-fine-composition-and-technological-properties-of-milk
#10
V Bonfatti, D Vicario, L Degano, A Lugo, P Carnier
The aim of this study was to compare the common method of exploiting infrared spectral data in animal breeding; that is, estimating the breeding values for the traits predicted by infrared spectroscopy, and an alternative approach based on the direct use of spectral information (direct prediction, DP) to predict the estimated breeding values (EBV). Traits were pH, milk coagulation properties, contents of the main casein and whey protein fractions, cheese yield measured by micro-cheese making, lactoferrin, Ca, and fat composition...
January 18, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28109597/weather-influences-feed-intake-and-feed-efficiency-in-a-temperate-climate
#11
Davina L Hill, Eileen Wall
A key goal for livestock science is to ensure that food production meets the needs of an increasing global population. Climate change may heighten this challenge through increases in mean temperatures and in the intensity, duration, and spatial distribution of extreme weather events, such as heat waves. Under high ambient temperatures, livestock are expected to decrease dry matter intake (DMI) to reduce their metabolic heat production. High yielding dairy cows require high DMI to support their levels of milk production, but this may increase susceptibility to heat stress...
January 18, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28109493/serum-periostin-level-is-not-associated-with-allergic-rhinitis-or-allergic-sensitization-in-korean-children
#12
Dong Young Kim, Jeong Hong Kim, Keun-Hwa Lee, Seong-Chul Hong, Hye-Sook Lee, Ju Wan Kang
OBJECTIVES: Periostin is a matricellular protein, synthesized in the airway epithelium and induced by interleukin (IL)-4 and IL-13. The significance of periostin as a biomarker of T helper type 2 cell (Th2)-induced airway inflammation, and as a measure of the response to Th2-targeted therapy, has recently been highlighted. We explored the relationship between serum periostin and allergic rhinitis in Korean children. METHODS: Data for fifth and sixth grade children from six randomly selected elementary schools located in Jeju and Seogwipo City, Korea, were investigated...
February 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28109442/label-free-and-standard-free-absolute-quantitative-proteomics-using-the-total-protein-and-proteomic-ruler-approaches
#13
J R Wiśniewski
Understanding biological systems and their variation upon stimuli requires knowledge on their composition, primarily including information on organization and dynamics of proteomes. The total protein approach (TPA) is a label- and standard-free method for absolute protein quantitation of proteins using large-scale proteomic data. The method relies on the assumption that the total MS signal from all identified proteins in the dataset reflects-in a biochemical sense-the total protein and the MS signal from a single protein corresponds its abundance in the studied sample...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28109432/targeted-in-depth-quantification-of-signaling-using-label-free-mass-spectrometry
#14
P R Cutillas
Protein phosphorylation encodes information on the activity of kinase-driven signaling pathways that regulate cell biology. This chapter discusses an approach, named TIQUAS (targeted in-depth quantification of signaling), to quantify cell signaling comprehensively and without bias. The workflow-based on mass spectrometry (MS) and computational science-consists of targeting the analysis of phosphopeptides previously identified by shotgun liquid chromatography tandem MS (LC-MS/MS) across the samples that are being compared...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28109431/proteogenomics-recycling-public-data-to-improve-genome-annotations
#15
A McAfee, L J Foster
Massively parallel sequencing is revealing species genomes faster than ever before, but the value of the raw sequence is limited unless the genes can be accurately annotated. This is typically achieved using gene prediction algorithms which, despite continual improvement, still require substantial verification and refinement. For example, in silico methods struggle with annotating splice isoforms accurately and empirical methods are needed to refine and verify the initial bioinformatic gene predictions. RNA-seq is an excellent way to confirm exon-exon boundaries and transcript termini, while mass spectrometry (MS) offers definitive proof that a gene is translated and a secondary means of confirming exon expression, protein termini, and posttranslational modifications...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28109425/beyond-the-natural-proteome-nondegenerate-saturation-mutagenesis-methodologies-and-advantages
#16
M M Ferreira Amaral, L Frigotto, A V Hine
Beyond the natural proteome, high-throughput mutagenesis offers the protein engineer an opportunity to "tweak" the wild-type activity of a protein to create a recombinant protein with required attributes. Of the various approaches available, saturation mutagenesis is one of the core techniques employed by protein engineers, and in recent times, nondegenerate saturation mutagenesis is emerging as the approach of choice. This review compares the current methodologies available for conducting nondegenerate saturation mutagenesis with traditional, degenerate saturation and briefly outlines the options available for screening the resulting libraries, to discover a novel protein with the required activity and/or specificity...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28109113/-effect-of-sodium-phenylbutyrate-on-the-sensitivity-of-pc3-dtx-resistant-prostate-cancer-cells-to-docetaxel
#17
Ya-Wen Xu, Shao-Bo Zheng, Bin-Sheng Chen, Yong Wen, Shan-Wen Zhu
OBJECTIVE: To investigate the effect of sodium phenylbutyrate (SPB) in modulating docetaxel resistance in human prostate cancer cells in vitro. METHODS: A PC3/docetaxel-resistant human prostate cancer cell line PC3/DTX was induced and examined for proliferation, viability, and cell inhibition rate in the presence of SPB. The concentration of concentration of docetaxel required to kill 50% of PC3/DTX cells incubated with 0, 1, 2, and 4 mmol/L SPB was determined using MTT assay...
2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28108976/phenotypic-and-biochemical-alterations-in-relation-to-mt2-gene-expression-in-plantago-ovata-forsk-under-zinc-stress
#18
Paulami Pramanick, Anindita Chakraborty, Sarmistha Sen Raychaudhuri
Plantago ovata Forsk is an annual herb with immense medicinal importance, the seed and husk of which is used in the treatment of chronic constipation, irritable bowel syndrome, diarrhea since ancient times. Zinc, an essential metal, is required by plants as they form important components of zinc finger proteins and also aid in synthesis of photosynthetic pigments such as chlorophyll. However, in excess amount Zn causes chlorosis of leaf and shoot tissues and generate reactive oxygen species. The present study is aimed at investigating the changes in expression levels of MT2 gene in Plantago ovata under zinc stress...
January 20, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/28108864/is-raf1-protein-from-synechocystis-sp-pcc-6803-really-needed-in-the-cyanobacterial-rubisco-assembly-process
#19
Piotr Kolesinski, Malgorzata Rydzy, Andrzej Szczepaniak
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is responsible for carbon dioxide conversion during photosynthesis and, therefore, is the most important protein in biomass generation. Modifications of this biocatalyst toward improvements in its properties are hindered by the complicated and not yet fully understood assembly process required for the formation of active holoenzymes. An entire set of auxiliary factors, including chaperonin GroEL/GroES and assembly chaperones RbcX or Rubisco accumulation factor 1 (RAF1), is involved in the folding and subsequent assembly of Rubisco subunits...
January 20, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28108828/a-novel-biomarker-based-preoperative-prognostic-grading-system-for-predicting-survival-after-surgery-for-intrahepatic-cholangiocarcinoma
#20
Tomoaki Yoh, Satoru Seo, Etsuro Hatano, Kojiro Taura, Hiroaki Fuji, Yoshinobu Ikeno, Yukihiro Okuda, Kentaro Yasuchika, Toshimi Kaido, Hideaki Okajima, Shinji Uemoto
BACKGROUND: Although treatment strategies for intrahepatic cholangiocarcinoma (ICC) are shifting towards multidisciplinary approaches, preoperative radiographic methods for identifying patients requiring further therapy are unclear. This study was designed to establish a prognostic grading system using preoperatively available objective biomarkers. METHODS: A novel preoperative prognostic grading system for predicting survival after surgery for ICC was developed from multivariate analysis of 134 ICC patients who underwent surgery between 1996 and 2015 using preoperatively available biomarkers...
January 20, 2017: Annals of Surgical Oncology
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