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https://www.readbyqxmd.com/read/28927090/downregulation-of-pdcd4-by-mir-21-suppresses-tumor-transformation-and-proliferation-in-a-nude-mouse-renal-cancer-model
#1
Haixin Yuan, Shiyong Xin, Yaoping Huang, Yingfan Bao, Hao Jiang, Liqing Zhou, Xiaoqiang Ren, Liang Li, Qian Wang, Jianguo Zhang
Programmed cell death 4 (PDCD4) is known to suppress neoplastic transformation, cell proliferation and metastasis, and to be downregulated by microRNA-21 (miR-21) in renal cell carcinoma (RCC) cell lines and tissues. The aim of the present study was to investigate the roles of and association between PDCD4 and miR-21 in a nude mouse renal cancer model. A total of 24 BALB/c male nude mice were randomly assigned into the following three groups: Negative control (NC; n=8), miR-21 inhibitor (n=8) and miR-21 mimic (n=8)...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28925484/mir-22-inhibits-lung-cancer-cell-emt-and-invasion-through-targeting-snail
#2
K Zhang, X-Y Li, Z-M Wang, Z-F Han, Y-H Zhao
OBJECTIVE: Snail is an important factor in regulating epithelial mesenchymal transition (EMT). Its elevation is related to the enhancement of lung cancer invasion. MicroRNA-22 (MiR-22) plays a role in regulating lung cancer cell invasion. Bioinformatics analysis showed the complementary binding site between miR-22 and Snail. This study aimed to investigate the role of miR-22 in regulating Snail and affecting lung cancer cell invasion and metastasis. MATERIALS AND METHODS: Dual luciferase assay confirmed the targeted relationship between miR-22 and Snail...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28922229/micrornas-as-clinical-biomarkers-and-therapeutic-tools-in-perioperative-medicine
#3
Simone Kreth, Max Hübner, Ludwig Christian Hinske
Over the past decade, evolutionarily conserved, noncoding small RNAs-so-called microRNAs (miRNAs)-have emerged as important regulators of virtually all cellular processes. miRNAs influence gene expression by binding to the 3'-untranslated region of protein-coding RNA, leading to its degradation and translational repression. In medicine, miRNAs have been revealed as novel, highly promising biomarkers and as attractive tools and targets for novel therapeutic approaches. miRNAs are currently entering the field of perioperative medicine, and they may open up new perspectives in anesthesia, critical care, and pain medicine...
September 14, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28864779/rnai-mediated-tcf-3-gene-silencing-inhibits-proliferation-of-eca-109-esophageal-cancer-cells-by-inducing-apoptosis
#4
Jie Ma, Xian-Bin Wang, Rui Li, Shu-Hong Xuan, Fang Wang, Xin-Hua Li, Zhi-Ping Zhang, Lu Tan, Lian Li
Esophageal cancer (EC) remains an important health problem in China. In this study, through the use of short interfering RNA (siRNA), specific gene knockdown of transcription factor 3 gene (TCF-3) was achieved in vitro and the effect of TCF-3 gene on human EC Eca-109 cell proliferation and apoptosis. Eca-109 cells were treated using negative control (NC) of siRNA against TCF-3 (siTCF-3) and siTCF-3 group. Colony formation assay was used to detect the colony formation ability of Eca-109 cells. MTT assay was used to measure the cell growth and viability, whereas BrDU assay was used to evaluate cell proliferation, and flow cytometry (FCM) to assess cell apoptosis...
September 1, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28857242/down-regulation-of-the-long-noncoding-rna-hox-transcript-antisense-intergenic-rna-inhibits-the-occurrence-and-progression-of-glioma
#5
Bin Yang, Zhen-Yu Wei, Bang-Qing Wang, Hua-Chao Yang, Jun-Yi Wang, Xing-Yao Bu
This study aims to explore the role of long noncoding RNA (lncRNA)-HOX transcript antisense intergenic RNA (HOTAIR) in the occurrence and progression of glioma. Fresh glioma and normal brain tissues were classified into a glioma group (n = 67) and a normal group (n = 64) respectively. U87 cells were assigned into the blank, sh-NC and sh-HOTAIR groups. Quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to determine HOTAIR expression. Cell proliferation, cell cycle and cell apoptosis rates were detected by cell counting kit-8 (CCK-8) and flow cytometry (FCM)...
August 31, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28853853/bna-nc-gapmers-revert-splicing-and-reduce-rna-foci-with-low-toxicity-in-myotonic-dystrophy-cells
#6
Kassie S Manning, Ashish N Rao, Miguel Castro, Thomas A Cooper
Myotonic dystrophy type 1 (DM1) is a multisystemic disease caused by an expanded CTG repeat in the 3' UTR of the dystrophia myotonica protein kinase (DMPK) gene. Short, DNA-based antisense oligonucleotides termed gapmers are a promising strategy to degrade toxic CUG expanded repeat (CUGexp) RNA. Nucleoside analogs are incorporated to increase gapmer affinity and stability; however, some analogs also exhibit toxicity. In this study, we demonstrate that the 2',4'-BNA(NC)[NMe] (BNA(NC)) modification is a promising nucleoside analog with high potency similar to 2',4'-LNA (LNA)...
September 5, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28843546/ljungan-sebokele-like-picornavirus-in-birds-of-prey-common-kestrel-falco-tinnunculus-and-red-footed-falcon-f-vespertinus
#7
Péter Pankovics, Ákos Boros, Róbert Mátics, Beatrix Kapusinszky, Eric Delwart, Gábor Reuter
Ljungan and Sebokele viruses are thought to be rodent-borne (picorna)viruses in the genus Parechovirus. Using random amplification and next generation sequencing method a novel Ljungan/Sebokele-like picornavirus was identified in birds of prey. Viral RNA was detected in total of 1 (9%) of the 11 and 2 (28.6%) of the 7 faecal samples from common kestrels and red-footed falcons in Hungary, respectively. High faecal viral RNA load (4.77×10(6) genomic copies/ml) measured by qPCR. The complete genome of picornavirus strain falcon/HA18_080/2014/HUN (KY645497) is 7964-nucleotide (nt) long including a 867-nt 5'end and a 101-nt 3'end (excluding the poly(A)-tail)...
August 24, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28820179/effects-of-dck-knockdown-on-proliferation-apoptosis-and-tumorigenicity-in-vivo-of-cervical-cancer-hela-cells
#8
Q-Y Shang, C-S Wu, H-R Gao
The present study explored the effect that deoxycytidine kinase (DCK) knockdown had on proliferation, apoptosis and tumorigenicity in vivo of cervical cancer HeLa cells. Human cervical cancer HeLa cells that had received no prior treatment were selected from the HeLa group. The HeLa-negative control (NC) group consisted of cells that had undergone an empty vector treatment, and finally the HeLa-short hairpin RNA (shRNA) group included cells that were treated by means of shRNA-DCK expression. DCK expressions were evaluated by quantitative real-time polymerase chain reaction in addition to western blotting assays...
August 18, 2017: Cancer Gene Therapy
https://www.readbyqxmd.com/read/28804566/microrna-93-inhibits-apoptosis-and-promotes-proliferation-invasion-and-migration-of-renal-cell-carcinoma-achn-cells-via-the-tgf-%C3%AE-smad-signaling-pathway-by-targeting-runx3
#9
Li-Jie Liu, Jian-Jun Yu, Xiao-Lin Xu
We investigated the ability of microRNA-93 (miR-93) to influence proliferation, invasion, migration, and apoptosisofrenal cell carcinoma (RCC) cells via transforming growth factor-β/solvated metal atom dispersed (TGF-β/Smad) signaling by targeting runt-related transcription factor 3 (RUNX3). RCC tissues with corresponding adjacent normal tissues were collected from 249 RCC patients. And normal renal tissues were collected from patients without RCC who received nephrectomy. The RCC cell line ACHN was treated with miR-93 mimic, mimic-negative control (NC), miR-93 inhibitor, inhibitor-NC, and miR-93 inhibitor + small interfering RNA (siRNA) against RUNX3 (si-RUNX3)...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28800319/mir-503-3p-induces-apoptosis-of-lung-cancer-cells-by-regulating-p21-and-cdk4-expression
#10
Yi Sun, Li Li, Shigang Xing, Yinghua Pan, Yunxiang Shi, Linghua Zhang, Qiang Shen
Studies have shown that microRNAs (miRNAs) can promote or suppress tumor growth and therefore act as targets for cancer therapy. Hsa-miR-503-5p, a mature miRNA derived from 5' ends of pre-miR-503, has been proved to regulate cell proliferation, transformation, migration and invasion. However, the biological function of miR-503-3p derived from 3' ends of pre-miR-503 has never been reported. In current study, we found that miR-503-3p inhibits lung cancer cell viability and induces cell apoptosis. To better understand the molecular mechanism underlying the miR-503-3p participating in this process, PCR array and RNA-sequencing (RNA-seq) were performed and some differential expression genes were discovered between NC and miR-503-3p treated groups...
July 28, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28780351/down-regulation-of-lncrna-kcnq1ot1-protects-against-myocardial-ischemia-reperfusion-injury-following-acute-myocardial-infarction
#11
Xin Li, Yingnan Dai, Shujun Yan, Yanli Shi, Baihe Han, Jingxiu Li, Li Cha, Jianjun Mu
This study aimed to investigate the protective effects of long non-coding RNA KCNQ1OT1 against myocardial ischemia/reperfusion (I/R) injury following acute myocardial infarction, as well as its regulatory mechanism. We used the cardiac muscle H9c2 cells under condition of oxygen glucose deprivation followed by reperfusion (OGD/R) to induce myocardial I/R injury. Then H9C2 cells were transfected with si-NC, si-KCNQ1OT1, pc-NC, pc-KCNQ1OT1, si-AdipoR1 and si-AdipoR2, respectively. The myocardial cell viability and apoptosis were respectively detected...
September 30, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28777253/mir-30e-attenuates-isoproterenol-induced-cardiac-fibrosis-through-suppressing-snai1-tgf%C3%AE-signaling
#12
Zhang Wenqi, Chang Hong, Zhang Hexun, Zhang Lei
BACKGROUND: MicroRNAs (miRNAs) are a class of small RNA molecules that inhibit protein expression through either degradation of mRNA or interference with protein translation. Our previous work suggested an involvement of miR-30e in myocardial fibrosis; however, the exact role of miR-30e in the pathogenesis of cardiac fibrosis and the underlying mechanisms are not known. METHODS: Male Sprague Dawley rats were treated with isoproterenol to induce cardiac remodeling and fibrosis and treated with either miR-30e agomir (AG) or antagomir and respective controls...
August 4, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28765930/e%C3%A2-cadherin-involvement-in-human-lens-epithelial-cell-transdifferentiation-may-be-associated-with-n%C3%A2-cadherin
#13
Lei Huang, Jie Jiang, Qiang Guo, Hongbin Yang
E-cadherin, β-catenin and N‑cadherin serve key roles in the epithelial‑to‑mesenchymal transition (EMT) that leads to human lens epithelial cell (LEC) transdifferentiation and subsequent cataract formation. The present study aimed to investigate the role of E‑cadherin in LEC transdifferentiation. SRA01/04 human LECs were transfected with E‑cadherin short interfering (si)RNA (E‑cadherin siRNA group), negative control siRNA (NC group) or the transfection regent Lipofectamine 2000 (blank group). Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to detect mRNA expression levels of E‑cadherin, N‑cadherin and β‑catenin, and western blot analysis was performed to measure the protein expression levels in the three groups...
October 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28762650/ancient-and-modern-hints-of-a-core-post-transcriptional-network-driving-chemotherapy-resistance-in-ovarian-cancer
#14
REVIEW
Sarah Blagden, Mai Abdel Mouti, James Chettle
RNA-binding proteins (RBPs) and noncoding (nc)RNAs (such as microRNAs, long ncRNAs, and others) cooperate within a post-transcriptional network to regulate the expression of genes required for many aspects of cancer behavior including its sensitivity to chemotherapy. Here, using an RBP-centric approach, we explore the current knowledge surrounding contributers to post-transcriptional gene regulation (PTGR) in ovarian cancer and identify commonalities that hint at the existence of an evolutionarily conserved core PTGR network...
August 1, 2017: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/28739722/grp78-participates-in-pca3-regulated-prostate-cancer-progression
#15
Chunhua Lin, Jiahui Wang, Yanwei Wang, Peng Zhu, Xin Liu, Ning Li, Juan Liu, Luxin Yu, Wenting Wang
BACKGROUND/AIM: The human prostate cancer antigen 3 (PCA3) is a long non-coding RNA (lncRNA) commonly used as a diagnostic marker for prostate cancer (PCa). Herein we investigated the cellular function of PCA3 in PCa and its potential mechanism. MATERIALS AND METHODS: PCA3 was overexpressed in a PC3 cell line (PC3(PCA+)) and cell proliferation, migration, invasion and apoptosis were compared to those of control cells (PC3(NC)). Differentially expressed proteins were identified by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28726630/dissection-of-specific-binding-of-hiv-1-gag-to-the-packaging-signal-in-viral-rna
#16
Mauricio Comas-Garcia, Siddhartha Ak Datta, Laura Baker, Rajat Varma, Prabhakar R Gudla, Alan Rein
Selective packaging of HIV-1 genomic RNA (gRNA) requires the presence of a cis-acting RNA element called the 'packaging signal' (Ψ). However, the mechanism by which Ψ promotes selective packaging of the gRNA is not well understood. We used fluorescence correlation spectroscopy and quenching data to monitor the binding of recombinant HIV-1 Gag protein to Cy5-tagged 190-base RNAs. At physiological ionic strength, Gag binds with very similar, nanomolar affinities to both Ψ-containing and control RNAs. We challenged these interactions by adding excess competing tRNA; introducing mutations in Gag; or raising the ionic strength...
July 20, 2017: ELife
https://www.readbyqxmd.com/read/28636115/sirna-mediated-down-regulation-of-clic4-gene-inhibits-cell-proliferation-and-accelerates-cell-apoptosis-of-mouse-liver-cancer-hca-f-and-hca-p-cells
#17
Qiu-Yun Yu, Xin-Feng Zhou, Qing Xia, Jia Shen, Jia Yan, Jiu-Ting Zhu, Xiang Li, Ming Shu
This study explored the effects involved in silencing CLIC4 on apoptosis and proliferation of mouse liver cancer Hca-F and Hca-P cells. A CLIC4-target small interfering RNA (siRNA) was designed to compound into two individual complementary oligonucleotide chains. A process of annealing and connection to a pSilencer vector was followed by transfection with Hca-F and Hca-P cells. Quantitative real-time polymerase chain reaction and western blotting techniques were used to determine CLIC4 mRNA and protein expressions...
June 21, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28635671/rab33b-controls-hepatitis-b-virus-assembly-by-regulating-core-membrane-association-and-nucleocapsid-processing
#18
Christina Bartusch, Tatjana Döring, Reinhild Prange
Many viruses take advantage of cellular trafficking machineries to assemble and release new infectious particles. Using RNA interference (RNAi), we demonstrate that the Golgi/autophagosome-associated Rab33B is required for hepatitis B virus (HBV) propagation in hepatoma cell lines. While Rab33B is dispensable for the secretion of HBV subviral envelope particles, its knockdown reduced the virus yield to 20% and inhibited nucleocapsid (NC) formation and/or NC trafficking. The overexpression of a GDP-restricted Rab33B mutant phenocopied the effect of deficit Rab33B, indicating that Rab33B-specific effector proteins may be involved...
June 21, 2017: Viruses
https://www.readbyqxmd.com/read/28630804/diversity-analysis-of-gut-microbiota-in-osteoporosis-and-osteopenia-patients
#19
Jihan Wang, Yangyang Wang, Wenjie Gao, Biao Wang, Heping Zhao, Yuhong Zeng, Yanhong Ji, Dingjun Hao
Some evidence suggests that bone health can be regulated by gut microbiota. To better understand this, we performed 16S ribosomal RNA sequencing to analyze the intestinal microbial diversity in primary osteoporosis (OP) patients, osteopenia (ON) patients and normal controls (NC). We observed an inverse correlation between the number of bacterial taxa and the value of bone mineral density. The diversity estimators in the OP and ON groups were increased compared with those in the NC group. Beta diversity analyses based on hierarchical clustering and principal coordinate analysis (PCoA) could discriminate the NC samples from OP and ON samples...
2017: PeerJ
https://www.readbyqxmd.com/read/28628915/mir-495-promotes-senescence-of-mesenchymal-stem-cells-by-targeting-bmi-1
#20
Xiujun Li, Yuxian Song, Dan Liu, Jiaojiao Zhao, Jingjing Xu, Jing Ren, Yali Hu, Zhiqun Wang, Yayi Hou, Guangfeng Zhao
BACKGROUND/AIMS: Mesenchymal stem cells (MSCs) play an important role in regulating angiogenesis and immune balance. Abnormal proliferation and function of MSCs were reported at maternal fetal interface in patients with pre-eclampsia (PE). Micro-RNA-495 was known to be upregulated in the MSCs derived from patients with PE. However, it is not clear whether the up-regulated miR-495 is related to the pathogenesis of PE. METHODS: We analyzed the expression of miR-495 in MSCs and umbilical cords derived from healthy pregnancies (NC) and PE, then we upregulated or downregulated the expression of miR-495 in MSCs derived from NC and tested the proliferation, apoptosis, migration, invasion, tube formation and senescence...
2017: Cellular Physiology and Biochemistry
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