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https://www.readbyqxmd.com/read/29350680/effects-of-microrna-135a-on-the-epithelial-mesenchymal-transition-migration-and-invasion-of-bladder-cancer-cells-by-targeting-gsk3%C3%AE-through-the-wnt-%C3%AE-catenin-signaling-pathway
#1
Xia-Wa Mao, Jia-Quan Xiao, Zhong-Yi Li, Yi-Chun Zheng, Nan Zhang
This study investigated the effects of microRNA-135a (miR-135a) targeting of glycogen synthase kinase 3β (GSK3β) on the epithelial-mesenchymal transition (EMT), migration and invasion of bladder cancer (BC) cells by mediating the Wnt/β-catenin signaling pathway. BC and adjacent normal tissues were collected from 165 BC patients. Western blotting and quantitative real-time PCR were used to detect the expression of GSK3β, β-catenin, cyclinD1, E-cadherin, vimentin and miR-135a in BC tissues and cells. Cells were assigned to blank, negative control (NC), miR-135a mimics, miR-135a inhibitors, small interfering RNA (siRNA)-GSK3β or miR-135a inhibitors+siRNA-GSK3β groups...
January 19, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29347911/micrornas-associated-with-early-neural-crest-development-in-xenopus-laevis
#2
Nicole J Ward, Darrell Green, Janet Higgins, Tamas Dalmay, Andrea Münsterberg, Simon Moxon, Grant N Wheeler
BACKGROUND: The neural crest (NC) is a class of transitory stem cell-like cells unique to vertebrate embryos. NC cells arise within the dorsal neural tube where they undergo an epithelial to mesenchymal transition in order to migrate and differentiate throughout the developing embryo. The derivative cell types give rise to multiple tissues, including the craniofacial skeleton, peripheral nervous system and skin pigment cells. Several well-studied gene regulatory networks underpin NC development, which when disrupted can lead to various neurocristopathies such as craniofrontonasal dysplasia, DiGeorge syndrome and some forms of cancer...
January 18, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29346185/higher-art-adherence-is-associated-with-lower-systemic-inflammation-in-treatment-na%C3%A3-ve-ugandans-who-achieve-virologic-suppression
#3
Jose R Castillo-Mancilla, Mary Morrow, Yap Boum, Helen Byakwaga, Jessica E Haberer, Jeffrey N Martin, David Bangsberg, Samantha Mawhinney, Nicholas Musinguzi, Yong Huang, Russell P Tracy, Tricia H Burdo, Kenneth Williams, Conrad Muzoora, Peter W Hunt, Mark J Siedner
BACKGROUND: Residual systemic inflammation persists despite suppressive antiretroviral therapy (ART) and is associated with non-AIDS clinical outcomes. We aimed to evaluate the association between ART adherence and inflammation in Ugandans living with HIV who were predominantly receiving nevirapine-based ART with a thymidine analog backbone and were virologically suppressed by conventional assays. METHODS: Plasma concentrations of interleukin-6 (IL-6), D-dimer, soluble (s)CD14, sCD163, the kynurenine/tryptophan (K/T) ratio, in addition to CD8 T-cell activation, were measured at baseline and 6 months after ART initiation in treatment-naïve adults who achieved an undetectable plasma HIV RNA (<400 copies/mL) at their 6-month visit...
January 16, 2018: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/29333768/-effects-of-geranylgeranyltransferase-%C3%A2-gene-silencing-by-rna-interference-on-the-migration-and-invasion-of-tongue-carcinoma
#4
Chen Zhenggang, Wang Shuren, Li Jinghua, Han Jinhong, Wang Qimin, Tong Lei, Liu Wenjun, Yang Fang, Guo Qingyuan, Guo Dawei, Wang Ying
OBJECTIVE: RNA interference was used to silence geranylgeranyltransferase Ⅰ(GGTase-Ⅰ) in vitro and to study the effect of GGTase-Ⅰ on the migration and invasion of tongue squamous cancer cells. METHODS: Three small interfering RNAs (siRNA) were designed according to the GGTase-Ⅰ sequence by Genebank and were transfected into tongue squamous cancer cells Cal-27 to knock down GGTase-Ⅰ expression. The tested cells were divided into three groups, as follows: the RNA-interfered groups (GGTase-Ⅰ siRNA1, GGTase-Ⅰ siRNA 2, GGTase-Ⅰ siRNA 3), a negative control group (disrupted by random sequence NC-siRNA), and a blank control group...
December 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29332167/non-coding-rnas-and-plant-male-sterility-current-knowledge-and-future-prospects
#5
REVIEW
Ankita Mishra, Abhishek Bohra
Latest outcomes assign functional role to non-coding (nc) RNA molecules in regulatory networks that confer male sterility to plants. Male sterility in plants offers great opportunity for improving crop performance through application of hybrid technology. In this respect, cytoplasmic male sterility (CMS) and sterility induced by photoperiod (PGMS)/temperature (TGMS) have greatly facilitated development of high-yielding hybrids in crops. Participation of non-coding (nc) RNA molecules in plant reproductive development is increasingly becoming evident...
January 13, 2018: Plant Cell Reports
https://www.readbyqxmd.com/read/29327503/noncoding-rnas-in-alzheimer-s-disease
#6
REVIEW
M Laura Idda, Rachel Munk, Kotb Abdelmohsen, Myriam Gorospe
Alzheimer's disease (AD) is a progressive neurodegenerative disorder and the main cause of dementia among the elderly worldwide. Despite intense efforts to develop drugs for preventing and treating AD, no effective therapies are available as yet, posing a growing burden at the personal, medical, and socioeconomic levels. AD is characterized by the production and aggregation of amyloid β (Aβ) peptides derived from amyloid precursor protein (APP), the presence of hyperphosphorylated microtubule-associated protein Tau (MAPT), and chronic inflammation leading to neuronal loss...
January 12, 2018: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/29326080/light-triggerable-formulations-for-the-intracellular-controlled-release-of-biomolecules
#7
REVIEW
Miguel M Lino, Lino Ferreira
New therapies based on the use of biomolecules [e.g., proteins, peptides, and non-coding (nc)RNAs] have emerged during the past few years. Given their instability, adverse effects, and limited ability to cross cell membranes, delivery systems are required to fully reveal their biological potential. Sophisticated nanoformulations responsive to light offer an excellent opportunity for the controlled release of these biomolecules, enabling the control of timing, duration, location, and dosage. In this review, we discuss the design principles for the delivery of biomolecules, in particular proteins and RNA-based therapeutics, by light-triggerable formulations...
January 8, 2018: Drug Discovery Today
https://www.readbyqxmd.com/read/29316975/inducible-t-cell-co-stimulators-regulate-the-proliferation-and-invasion-of-human-hepatocellular-carcinoma-hepg2-cells
#8
Yaning Wei, Yanan Wang, Aimin Zang, Yanhong Shang, Zizheng Song, Zhiyu Wang, Yangyang Wang, Hua Yang
BACKGROUND: This study determined the regulatory effects of inducible T-cell co-stimulators (ICOS) in human hepatocellular carcinoma HepG2 cells using a RNA interference (RNAi) technique. METHODS: A RNAi technique was used to knockdown the expression of ICOS. ICOS expression after knockdown was detected as mRNA and protein levels by RT-PCR and Western blot, respectively. A MTT colorimetric assay was used to detect cell proliferation, and the Transwell assay was used to detect cell invasion...
January 9, 2018: Biological Research
https://www.readbyqxmd.com/read/29300972/a-recurrent-de-novo-missense-mutation-in-ubtf-causes-developmental-neuroregression
#9
Camilo Toro, Roderick T Hori, May Christine V Malicdan, Cynthia J Tifft, Amy Goldstein, William A Gahl, David R Adams, Fauni Harper, Lynne A Wolfe, Jianfeng Xiao, Mohammad M Khan, Jun Tian, Kevin A Hope, Lawrence T Reiter, Michel G Tremblay, Thomas Moss, Alexis L Franks, Chris Balak, Mark S LeDoux
UBTF (upstream binding transcription factor) exists as two isoforms; UBTF1 regulates rRNA transcription by RNA polymerase 1, whereas UBTF2 regulates mRNA transcription by RNA polymerase 2. Herein, we describe 4 patients with very similar patterns of neuroregression due to recurrent de novo mutations in UBTF (GRCh37/hg19, NC_000017.10:g.42290219C>T, NM_014233.3:c.628G>A) resulting in the same amino acid change in both UBTF1 and UBTF2 (p.Glu210Lys [p.E210K]). Disease onset in our cohort was at 2.5 to 3 yrs and characterized by slow progression of global motor, cognitive and behavioral dysfunction...
January 2, 2018: Human Molecular Genetics
https://www.readbyqxmd.com/read/29300742/component-tree-analysis-of-cystovirus-%C3%AF-6-nucleocapsid-cryo-em-single-particle-reconstructions
#10
Lucas M Oliveira, Ze Ye, Al Katz, Alexandra Alimova, Hui Wei, Gabor T Herman, Paul Gottlieb
The 3-dimensional structure of the nucleocapsid (NC) of bacteriophage φ6 is described utilizing component tree analysis, a topological and geometric image descriptor. The component trees are derived from density maps of cryo-electron microscopy single particle reconstructions. Analysis determines position and occupancy of structure elements responsible for RNA packaging and transcription. Occupancy of the hexameric nucleotide triphosphorylase (P4) and RNA polymerase (P2) are found to be essentially complete in the NC...
2018: PloS One
https://www.readbyqxmd.com/read/29294597/microrna-16-inhibits-cell-proliferation-and-migration-by-targeting-heat-shock-protein-70-in-heat-denatured-dermal-fibroblasts
#11
Chun Zhang, Jinhua Dai, Youfen Fan, Xianghui He, Renxiong Wei
Background/Aims: This study aimed to investigate the precise mechanism and function of miR-16 in heat-denatured primary human dermal fibroblasts. Methods: Primary human dermal fibroblasts were separated from normal human skin samples. Under heat stress, the levels of miR-16 and heat shock protein 70 (HSP70) were detected in primary human dermal fibroblasts by quantitative real-time polymerase chain reaction (qRT-PCR). Next, heat-denatured cells were transfected with synthetic scrambled negative control (NC) RNA (NC group), miR-16 mimics, miR-16 inhibitor or miR-16 inhibitor accompanied by small interfering RNA targeting HSP70, then the mRNA level of HSP70 was detected by qRT-PCR, cell proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and AlamarBlue assay, cell migration was examined by Transwell assay and cell apoptosis was assessed by transferase dUTP (deoxyuridine triphosphate) nick end labeling (TUNEL) assay...
January 5, 2018: Korean Journal of Internal Medicine
https://www.readbyqxmd.com/read/29260518/-effects-of-lncrna-h19-on-proliferation-of-human-colorectal-cancer-sw620-cells
#12
Xiao-Lei Huo, Zhen Pei, Yong-Zhi Li, Ling-Na Han, Xiang-Yang Tian, Yi-Qiang Zhang
OBJECTIVE: To determine the expression level of long non-coding RNA (lncRNA) imprinted maternallyexpressed transcript (H19) in colorectal cancer tissues and its effect on proliferation of colorectal cancer SW620 cells. METHODS: Real-time quantitative PCR (qRT-PCR) was applied to detect the expression of H19 in 20 paired tumor tissues and adjacent normal tissues,and in normal NCM460 cells and colorectal cancer SW480,HCT116 and SW620 cells. The specific small interfering RNA for H19 (si-H19 group) or negative control sequence (si-NC group) were transfected into SW620 cells...
November 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/29239893/switch-from-tenofovir-disoproxil-fumarate-combination-to-dolutegravir-plus-rilpivirine-improves-parameters-of-bone-health
#13
Grace A Mccomsey, Sergio Lupo, David Parks, Mónica Coronado Poggio, Joseph De Wet, Lesley P Kahl, Kostas Angelis, Brian Wynne, Kati Vandermeulen, Martin Gartland, Michael Cupo, Michael Aboud
OBJECTIVE: Bone mineral density (BMD) loss, a risk factor for osteoporosis, has been attributed to HIV infection and antiretroviral therapy (ART), including regimens containing tenofovir disoproxil fumarate. DESIGN: Study 202094 is an open-label, parallel-group, sub-study of the phase III SWORD-1 and SWORD-2 studies (ClincialTrials.gov identifier, NCT02478632). METHODS: HIV-1-infected adults with HIV-1 RNA < 50 copies per mL who received ART containing tenofovir disoproxil fumarate for ≥6 months were randomized to receive dolutegravir+rilpivirine or continue current ART regimen...
December 12, 2017: AIDS
https://www.readbyqxmd.com/read/29236319/effects-of-long-non-coding-rna-host2-on-cell-migration-and-invasion-by-regulating-microrna-let-7b-in-breast-cancer
#14
Peng-Wei Lu, Lin Li, Fang Wang, Yuan-Ting Gu
The study intends to investigate the effects of long non-coding RNA HOST2 (lncRNA HOST2) on cell migration and invasion by regulating microRNA let-7b (let-7b) in breast cancer. Breast cancer and adjacent normal tissues were collected from 98 patients with breast cancer. Breast cancer MCF-7 cells were divided into the blank, negative control (NC), pcDNA3-Mock, siHOST2, let-7b inhibitor, pcDNA3-HOST2, let-7b mimic, pcDNA3-HOST2 + let-7b mimic and siHOST2 + let-7b inhibitor groups. RT-qPCR was used to detect the mRNA expressions of HOST2, let-7b and c-Myc...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29226644/the-role-of-microrna-1207-5p-in-colorectal-cancer
#15
Xiaokun Wang, Xiaodong Wu
BACKGROUND: MicroRNA-1207-5p (MiR-1207-5p) has been identified as a tumor suppressor in many types of cancer. However, less research has been done with respect to the role of miR-1207-5p in colorectal cancer (CRC). The aim of our study was to analyze the role of miR-1207-5p in CRC. METHODS: Total RNA was extracted from clinical specimens and CRC cell line, and the expression of miR-1207-5p was examined by real-time PCR (q-PCR). The human CRC cell lines were transfected with the miR-1207-5p mimic, negative control (NC)...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29215742/effects-of-small-interfering-rna-targeting-tlr4-on-expressions-of-adipocytokines-in-obstructive-sleep-apnea-hyponea-syndrome-with-hypertension-in-a-rat-model
#16
Jing-Guo Wu, Nan Xun, Li-Jin Zeng, Zhen-Yu Li, Yan-Bing Liang, Hao Tang, Zhong-Fu Ma
We explored the effects of RNA interference-mediated silencing of TLR4 gene on expressions of adipocytokines in obstructive sleep apnea hyponea syndrome (OSAS) with hypertension in a rat model. Systolic blood pressure of caudal artery and physiological changes were observed when establishing rat models of OSAS with hypertension. Mature rat adipocytes were induced from separated and cultured primary rat adipocytes. To transfect rat mature adipocytes, TLR4 siRNA group and negative control (NC) siRNA group were established...
December 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29193300/rna-interference-mediated-knockdown-and-virus-induced-suppression-of-troponin-c-gene-adversely-affect-the-behavior-or-fitness-of-the-green-rice-leafhopper-nephotettix-cincticeps
#17
Hanhong Lan, Xiaojing Hong, Ranran Huang, Xin Lin, Qinghuang Li, Kaihui Li, Tao Zhou
The green rice leafhopper, Nephotettix cincticeps, is a major rice pest in Southeast Asia and Southern China. Novel control strategies must be explored to control the rice pest. Behavior or fitness regulation of insect by modulating the Troponin C (TnC) may be a novel strategy in the comprehensive management of the insect pest. However, characterizations and functions of TnC, especially regarding effect of its RNA interference-mediated gene knockdown on the behavior or fitness of N. cincticeps remain unknown...
November 30, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/29176398/the-role-of-the-globular-heads-of-the-c1q-receptor-in-paclitaxel-induced-human-ovarian-cancer-cells-apoptosis-by-a-mitochondria-dependent-pathway
#18
Kang-Tai Lv, Ling-Juan Gao, Xiangdong Hua, Fengshan Li, Yun Gu, Wei Wang
As a mitochondrial membrane protein, globular C1q receptor (gC1qR) can mediate a variety of biological responses. Our study aims to investigate the role of gC1qR in paclitaxel-induced apoptosis of human ovarian cancer cells and to elucidate its potential molecular mechanism. The level of gC1qR was examined using real-time PCR and western blot analyses. Human ovarian cancer cells viability, migration, and proliferation were detected using the water-soluble tetrazolium salt (WST-1) assay, the transwell assay, and H-thymidine incorporation into DNA (H-TdR) assay, respectively...
November 23, 2017: Anti-cancer Drugs
https://www.readbyqxmd.com/read/29163812/identification-of-metastasis-associated-micrornas-in-serum-from-rectal-cancer-patients
#19
Robin Mjelle, Kjersti Sellæg, Pål Sætrom, Liv Thommesen, Wenche Sjursen, Eva Hofsli
MicroRNAs (miRNAs) are promising prognostic and diagnostic biomarkers due to their high stability in blood. Here we investigate the expression of miRNAs and other noncoding (nc) RNAs in serum of rectal cancer patients. Serum from 96 rectal cancer patients was profiled using small RNA sequencing and expression of small RNAs was correlated with the clinicopathological characteristics of the patients. Multiple classes of RNAs were detected, including miRNAs and fragments of tRNAs, snoRNAs, long ncRNAs, and other classes of RNAs...
October 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/29154927/integrative-transcriptome-and-proteome-analyses-define-marked-differences-between-neospora-caninum-isolates-throughout-the-tachyzoite-lytic-cycle
#20
P Horcajo, D Xia, N Randle, E Collantes-Fernández, J Wastling, L M Ortega-Mora, J Regidor-Cerrillo
Neospora caninum is one of the main causes of transmissible abortion in cattle. Intraspecific variations in virulence have been widely shown among N. caninum isolates. However, the molecular basis governing such variability have not been elucidated to date. In this study label free LC-MS/MS was used to investigate proteome differences between the high virulence isolate Nc-Spain7 and the low virulence isolate Nc-Spain1H throughout the tachyzoite lytic cycle. The results showed greater differences in the abundance of proteins at invasion and egress with 77 and 62 proteins, respectively...
November 14, 2017: Journal of Proteomics
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