keyword
MENU ▼
Read by QxMD icon Read
search

nc RNA

keyword
https://www.readbyqxmd.com/read/28804566/microrna-93-inhibits-apoptosis-and-promotes-proliferation-invasion-and-migration-of-renal-cell-carcinoma-achn-cells-via-the-tgf-%C3%AE-smad-signaling-pathway-by-targeting-runx3
#1
Li-Jie Liu, Jian-Jun Yu, Xiao-Lin Xu
We investigated the ability of microRNA-93 (miR-93) to influence proliferation, invasion, migration, and apoptosisofrenal cell carcinoma (RCC) cells via transforming growth factor-β/solvated metal atom dispersed (TGF-β/Smad) signaling by targeting runt-related transcription factor 3 (RUNX3). RCC tissues with corresponding adjacent normal tissues were collected from 249 RCC patients. And normal renal tissues were collected from patients without RCC who received nephrectomy. The RCC cell line ACHN was treated with miR-93 mimic, mimic-negative control (NC), miR-93 inhibitor, inhibitor-NC, and miR-93 inhibitor + small interfering RNA (siRNA) against RUNX3 (si-RUNX3)...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28800319/mir-503-3p-induces-apoptosis-of-lung-cancer-cells-by-regulating-p21-and-cdk4-expression
#2
Yi Sun, Li Li, Shigang Xing, Yinghua Pan, Yunxiang Shi, Linghua Zhang, Qiang Shen
Studies have shown that microRNAs (miRNAs) can promote or suppress tumor growth and therefore act as targets for cancer therapy. Hsa-miR-503-5p, a mature miRNA derived from 5' ends of pre-miR-503, has been proved to regulate cell proliferation, transformation, migration and invasion. However, the biological function of miR-503-3p derived from 3' ends of pre-miR-503 has never been reported. In current study, we found that miR-503-3p inhibits lung cancer cell viability and induces cell apoptosis. To better understand the molecular mechanism underlying the miR-503-3p participating in this process, PCR array and RNA-sequencing (RNA-seq) were performed and some differential expression genes were discovered between NC and miR-503-3p treated groups...
July 28, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28780351/down-regulation-of-lncrna-kcnq1ot1-protects-against-myocardial-ischemia-reperfusion-injury-following-acute-myocardial-infarction
#3
Xin Li, Yingnan Dai, Shujun Yan, Yanli Shi, Baihe Han, Jingxiu Li, Li Cha, Jianjun Mu
This study aimed to investigate the protective effects of long non-coding RNA KCNQ1OT1 against myocardial ischemia/reperfusion (I/R) injury following acute myocardial infarction, as well as its regulatory mechanism. We used the cardiac muscle H9c2 cells under condition of oxygen glucose deprivation followed by reperfusion (OGD/R) to induce myocardial I/R injury. Then H9C2 cells were transfected with si-NC, si-KCNQ1OT1, pc-NC, pc-KCNQ1OT1, si-AdipoR1 and si-AdipoR2, respectively. The myocardial cell viability and apoptosis were respectively detected...
August 2, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28777253/mir-30e-attenuates-isoproterenol-induced-cardiac-fibrosis-through-suppressing-snai1-tgf%C3%AE-signaling
#4
Zhang Wenqi, Chang Hong, Zhang Hexun, Zhang Lei
BACKGROUND: MicroRNAs (miRNAs) are a class of small RNA molecules that inhibit protein expression through either degradation of mRNA or interference with protein translation. Our previous work suggested an involvement of miR-30e in myocardial fibrosis; however, the exact role of miR-30e in the pathogenesis of cardiac fibrosis and the underlying mechanisms are not known. METHODS: Male Sprague Dawley rats were treated with isoproterenol to induce cardiac remodeling and fibrosis and treated with either miR-30e agomir (AG) or antagomir and respective controls...
August 4, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28765930/e%C3%A2-cadherin-involvement-in-human-lens-epithelial-cell-transdifferentiation-may-be-associated-with-n%C3%A2-cadherin
#5
Lei Huang, Jie Jiang, Qiang Guo, Hongbin Yang
E-cadherin, β-catenin and N‑cadherin serve key roles in the epithelial‑to‑mesenchymal transition (EMT) that leads to human lens epithelial cell (LEC) transdifferentiation and subsequent cataract formation. The present study aimed to investigate the role of E‑cadherin in LEC transdifferentiation. SRA01/04 human LECs were transfected with E‑cadherin short interfering (si)RNA (E‑cadherin siRNA group), negative control siRNA (NC group) or the transfection regent Lipofectamine 2000 (blank group). Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to detect mRNA expression levels of E‑cadherin, N‑cadherin and β‑catenin, and western blot analysis was performed to measure the protein expression levels in the three groups...
August 2, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28762650/ancient-and-modern-hints-of-a-core-post-transcriptional-network-driving-chemotherapy-resistance-in-ovarian-cancer
#6
REVIEW
Sarah Blagden, Mai Abdel Mouti, James Chettle
RNA-binding proteins (RBPs) and noncoding (nc)RNAs (such as microRNAs, long ncRNAs, and others) cooperate within a post-transcriptional network to regulate the expression of genes required for many aspects of cancer behavior including its sensitivity to chemotherapy. Here, using an RBP-centric approach, we explore the current knowledge surrounding contributers to post-transcriptional gene regulation (PTGR) in ovarian cancer and identify commonalities that hint at the existence of an evolutionarily conserved core PTGR network...
August 1, 2017: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/28739722/grp78-participates-in-pca3-regulated-prostate-cancer-progression
#7
Chunhua Lin, Jiahui Wang, Yanwei Wang, Peng Zhu, Xin Liu, Ning Li, Juan Liu, Luxin Yu, Wenting Wang
BACKGROUND/AIM: The human prostate cancer antigen 3 (PCA3) is a long non-coding RNA (lncRNA) commonly used as a diagnostic marker for prostate cancer (PCa). Herein we investigated the cellular function of PCA3 in PCa and its potential mechanism. MATERIALS AND METHODS: PCA3 was overexpressed in a PC3 cell line (PC3(PCA+)) and cell proliferation, migration, invasion and apoptosis were compared to those of control cells (PC3(NC)). Differentially expressed proteins were identified by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28726630/dissection-of-specific-binding-of-hiv-1-gag-to-the-packaging-signal-in-viral-rna
#8
Mauricio Comas-Garcia, Siddhartha Ak Datta, Laura Baker, Rajat Varma, Prabhakar R Gudla, Alan Rein
Selective packaging of HIV-1 genomic RNA (gRNA) requires the presence of a cis-acting RNA element called the 'packaging signal' (Ψ). However, the mechanism by which Ψ promotes selective packaging of the gRNA is not well understood. We used fluorescence correlation spectroscopy and quenching data to monitor the binding of recombinant HIV-1 Gag protein to Cy5-tagged 190-base RNAs. At physiological ionic strength, Gag binds with very similar, nanomolar affinities to both Ψ-containing and control RNAs. We challenged these interactions by adding excess competing tRNA; introducing mutations in Gag; or raising the ionic strength...
July 20, 2017: ELife
https://www.readbyqxmd.com/read/28636115/sirna-mediated-down-regulation-of-clic4-gene-inhibits-cell-proliferation-and-accelerates-cell-apoptosis-of-mouse-liver-cancer-hca-f-and-hca-p-cells
#9
Qiu-Yun Yu, Xin-Feng Zhou, Qing Xia, Jia Shen, Jia Yan, Jiu-Ting Zhu, Xiang Li, Ming Shu
This study explored the effects involved in silencing CLIC4 on apoptosis and proliferation of mouse liver cancer Hca-F and Hca-P cells. A CLIC4-target small interfering RNA (siRNA) was designed to compound into two individual complementary oligonucleotide chains. A process of annealing and connection to a pSilencer vector was followed by transfection with Hca-F and Hca-P cells. Quantitative real-time polymerase chain reaction and western blotting techniques were used to determine CLIC4 mRNA and protein expressions...
June 21, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28635671/rab33b-controls-hepatitis-b-virus-assembly-by-regulating-core-membrane-association-and-nucleocapsid-processing
#10
Christina Bartusch, Tatjana Döring, Reinhild Prange
Many viruses take advantage of cellular trafficking machineries to assemble and release new infectious particles. Using RNA interference (RNAi), we demonstrate that the Golgi/autophagosome-associated Rab33B is required for hepatitis B virus (HBV) propagation in hepatoma cell lines. While Rab33B is dispensable for the secretion of HBV subviral envelope particles, its knockdown reduced the virus yield to 20% and inhibited nucleocapsid (NC) formation and/or NC trafficking. The overexpression of a GDP-restricted Rab33B mutant phenocopied the effect of deficit Rab33B, indicating that Rab33B-specific effector proteins may be involved...
June 21, 2017: Viruses
https://www.readbyqxmd.com/read/28630804/diversity-analysis-of-gut-microbiota-in-osteoporosis-and-osteopenia-patients
#11
Jihan Wang, Yangyang Wang, Wenjie Gao, Biao Wang, Heping Zhao, Yuhong Zeng, Yanhong Ji, Dingjun Hao
Some evidence suggests that bone health can be regulated by gut microbiota. To better understand this, we performed 16S ribosomal RNA sequencing to analyze the intestinal microbial diversity in primary osteoporosis (OP) patients, osteopenia (ON) patients and normal controls (NC). We observed an inverse correlation between the number of bacterial taxa and the value of bone mineral density. The diversity estimators in the OP and ON groups were increased compared with those in the NC group. Beta diversity analyses based on hierarchical clustering and principal coordinate analysis (PCoA) could discriminate the NC samples from OP and ON samples...
2017: PeerJ
https://www.readbyqxmd.com/read/28628915/mir-495-promotes-senescence-of-mesenchymal-stem-cells-by-targeting-bmi-1
#12
Xiujun Li, Yuxian Song, Dan Liu, Jiaojiao Zhao, Jingjing Xu, Jing Ren, Yali Hu, Zhiqun Wang, Yayi Hou, Guangfeng Zhao
BACKGROUND/AIMS: Mesenchymal stem cells (MSCs) play an important role in regulating angiogenesis and immune balance. Abnormal proliferation and function of MSCs were reported at maternal fetal interface in patients with pre-eclampsia (PE). Micro-RNA-495 was known to be upregulated in the MSCs derived from patients with PE. However, it is not clear whether the up-regulated miR-495 is related to the pathogenesis of PE. METHODS: We analyzed the expression of miR-495 in MSCs and umbilical cords derived from healthy pregnancies (NC) and PE, then we upregulated or downregulated the expression of miR-495 in MSCs derived from NC and tested the proliferation, apoptosis, migration, invasion, tube formation and senescence...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28628529/appraising-viral-load-thresholds-and-adherence-support-recommendations-in-the-world-health-organization-guidelines-for-detection-and-management-of-virologic-failure
#13
Suzanne M McCluskey, Yap Boum, Nicholas Musinguzi, Jessica E Haberer, Jeffrey N Martin, Peter W Hunt, Vincent C Marconi, David R Bangsberg, Mark J Siedner
BACKGROUND: The World Health Organization defines HIV virologic failure as two consecutive viral loads >1,000 copies/mL, measured 3-6 months apart with interval adherence support. We sought to empirically evaluate these guidelines using data from an observational cohort. SETTING: The Uganda AIDS Rural Treatment Outcomes study observed adults with HIV in southwestern Uganda from the time of antiretroviral therapy (ART) initiation, and monitored adherence with electronic pill bottles...
June 15, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28618405/upregulation-of-circulating-mir-195-3p-in-heart-failure
#14
Xia He, Jun Ji, Tao Wang, Ming-Bang Wang, Xiao-Ling Chen
OBJECTIVES: Many circulating microRNAs (miRs) have been shown to have potential biomarker effects in cardiovascular disease. We studied the dysregulation of circulating miR-195-3p in patients with heart failure (HF) to elucidate its value as a potential biomarker for HF. METHODS: Eight ischemic HF (IHF) patients, 8 nonischemic HF patients (NIHF), and 8 healthy volunteers (matched by age and sex - normal controls [NCs]) were chosen for miR sequencing. The plasma RNA was extracted, and a small RNA library of HF was established and then sequenced using next-generation sequencing (NGS) technology...
June 16, 2017: Cardiology
https://www.readbyqxmd.com/read/28598903/self-reported-difficulties-with-everyday-function-cognitive-symptoms-and-cognitive-function-in-people-with-hiv
#15
Rosanna Laverick, Lewis Haddow, Marina Daskalopoulou, Fiona Lampe, Richard Gilson, Andrew Speakman, Andrea Antinori, Tina Bruun, Anna Vassilenko, Simon Collins, Alison Rodger
BACKGROUND: We determined factors associated with self-reported decline in activities of daily living (ADLs) and symptoms of cognitive impairment in HIV positive (HIV+) adults in five European clinics. METHODS: HIV+ adults underwent computerized and pen-and-paper neuropsychological tests and questionnaires of cognitive symptoms and ADLs. We considered cognitive function in five domains, psychosocial factors and clinical parameters as potentially associated with symptoms...
June 7, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28598082/-expression-of-micro-rna-218-in-cervical-cancer-and-its-effect-on-proliferation-apoptosis-and-invasion-of-hela-cells
#16
Yu-Rong Shi, Jian Liu, Wei He, Yin Yang
OBJECTIVES: To investigate the expression and clinical significance of microRNA-218(miR-218)in human cervical cancer and the effects ofmiR-218 on proliferation, cell apoptosis and invasion of HeLa cells. METHODS: QRT-PCR was used to detect the expression ofmiR-218 in 23 cases of normal cervical tissues and 114 cases of cervical cancer, and the relationship between the expression and the clinicopathological features was analyzed; HeLa cells were devided into three groups: non transfection (control group), transfected with empty liposomes negative control goup (NC group), transfected with miR-218 mimic (miR-218M group)...
September 2016: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28577495/knockdown-of-long-noncoding-rna-ghet1-inhibits-cell-activation-of-gastric-cancer
#17
Hui Huang, Wenjun Liao, Xueqiang Zhu, Hao Liu, Lijun Cai
OBJECTION: The aim of this study was to evaluate the effects of lncRNA GHET1 in developing of Gastric Cancer. METHODS: Collecting the 20 gastric cancer patients and evaluated the pathological of adjacent and tumor tissues by HE stating. We analyzed the protein expression of Numb in gastric cancer (GC) tissues by using IHC and the gene expression of Numb and GHET1 in adjacent and GC tissues by RT-PCR. The AGS cells were divided into 3 groups: Control (Co), NC and shRNA groups...
August 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28539331/overexpression-oflongintergenicnoncoding-rna-linc00312inhibits-the-invasion-and-migration-of-thyroid-cancer-cells-by-down-regulating-microrna-197-3p
#18
Kai Liu, Wen Huang, Dan-Qing Yan, Qing Luo, Xiang Min
The study evaluated the ability of LINC00312 to influence the proliferation, invasion and migration of thyroid cancer (TC) cells by regulating microRNA-197-3p (miRNA-197-3p). TC  tissues and adjacent normal tissues were collected from 211 TC patients. K1 (papillary TC), SW579 (squamous TC) and 8505C (anaplastic TC) cell lines were assigned into a blank, negative control (NC), LINC00312 overexpression, miR-197-3p inhibitors and LINC00312 overexpression + miR-197-3p mimics group. The expression of LINC00312, miR-197-3p and p120 was measured using quantitative real time polymerase chain reaction (qRT-PCR) and western blotting...
May 24, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28516123/polysome-fractionation-to-analyze-mrna-distribution-profiles
#19
Amaresh C Panda, Jennifer L Martindale, Myriam Gorospe
Eukaryotic cells adapt to changes in external or internal signals by precisely modulating the expression of specific gene products. The expression of protein-coding genes is controlled at the transcriptional and post-transcriptional levels. Among the latter steps, the regulation of translation is particularly important in cellular processes that require rapid changes in protein expression patterns. The translational efficiency of mRNAs is altered by RNA-binding proteins (RBPs) and noncoding (nc)RNAs such as microRNAs (Panda et al...
February 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28502297/-knock-down-of-cadherin-17-inhibits-proliferation-and-promote-apoptosis-in-noscapine-resistant-human-sw480-colon-cancer-cells
#20
Zheng Han, Xiaodong Huang, Meng Liu, Jie Tan, Qingxi Zhu, Yanfen Wang, Yishan Cai, Aifang Chen, Xia Tian
Objective To investigate the effects of cadherin 17 (CDH17) on the proliferation and apoptosis of noscapine-resistant human SW480 colon cancer cells. Methods The level of CDH17 in noscapine-resistant human SW480 colon cancer cells was knocked down by small interfering RNA (siRNA), and the silence was confirmed by Western blotting and real-time quantitative PCR. Transfected SW480 cells were treated with noscapine, and then the proliferation and cell viability of SW480 cells were measured by MTT assay and plate clone formation assay, respectively; the apoptosis of SW480 cells was detected by flow cytometry combined with annexinV-FITC/PI staining; the expressions of poly-ADP-ribose polymerase (PARP) and caspase-3 were determined by Western blotting...
May 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
keyword
keyword
115798
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"