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https://www.readbyqxmd.com/read/29779946/pnpt1-release-from-mitochondria-during-apoptosis-triggers-decay-of-poly-a-rnas
#1
Xing Liu, Rui Fu, Youdong Pan, Karla F Meza-Sosa, Zhibin Zhang, Judy Lieberman
Widespread mRNA decay, an unappreciated feature of apoptosis, enhances cell death and depends on mitochondrial outer membrane permeabilization (MOMP), TUTases, and DIS3L2. Which RNAs are decayed and the decay-initiating event are unknown. Here, we show extensive decay of mRNAs and poly(A) noncoding (nc)RNAs at the 3' end, triggered by the mitochondrial intermembrane space 3'-to-5' exoribonuclease PNPT1, released during MOMP. PNPT1 knockdown inhibits apoptotic RNA decay and reduces apoptosis, while ectopic expression of PNPT1, but not an RNase-deficient mutant, increases RNA decay and cell death...
May 10, 2018: Cell
https://www.readbyqxmd.com/read/29771789/raltegravir-1200-mg-once-daily-vs-400-mg-twice-daily-with-emtricitabine-and-tenofovir-disoproxil-fumarate-for-previously-untreated-hiv-1-infection-week-96-results-from-oncemrk-a-randomized-double-blind-non-inferiority-trial
#2
Pedro Cahn, Paul E Sax, Kathleen Squires, Jean-Michel Molina, Winai Ratanasuwan, Mohammed Rassool, Mark Bloch, Xia Xu, Yan Zhou, Brenda Homony, Deborah Hepler, Hedy Teppler, George J Hanna, Bach-Yen Nguyen, Wayne Greaves
BACKGROUND: Raltegravir 1200mg (2x600mg tablets) once daily (QD) demonstrated non-inferior efficacy and similar safety to raltegravir 400mg BID at Week 48 of the ONCEMRK trial. Here we report the Week 96 results from this study. METHODS: ONCEMRK is a phase 3, multicenter, double-blind, non-inferiority trial comparing raltegravir 1200mg QD to raltegravir 400mg BID in treatment-naïve HIV-1-infected adults. Participants were assigned (2:1) to raltegravir 2x600mg QD or 400mg BID, both with emtricitabine and tenofovir disoproxil fumarate (FTC/TDF) for 96 weeks...
May 3, 2018: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/29755595/long-non-coding-rna-lsinct5-promotes-ovarian-cancer-cell-proliferation-migration-and-invasion-by-disrupting-the-cxcl12-cxcr4-signalling-axis
#3
Xingtao Long, Li Li, Qi Zhou, Haixia Wang, Dongling Zou, Dong Wang, Meng Lou, Weiqi Nian
Long stress-induced noncoding transcript 5 (LSINCT5) is a member of the LSINCT family, members of which are expressed during stress-induced cell formation and have also been reported to promote cancer progression. In the present study, the association between LSINCT5 expression and clinical significance was investigated and the biological function of LSINCT5 in epithelial ovarian cancer (EOC) was explored. LSINCT5 expression was examined in EOC tissues by reverse transcription-quantitative polymerase chain reaction and its association with clinicopathological factors was analysed...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29750314/screening-of-differentially-expressed-genes-in-male-idiopathic-osteoporosis-via-rna-sequencing
#4
Li Feng, Yan Wang, Jing Zhou, Baofang Tian, Bo Xia
As a type of osteoporosis (OP), male idiopathic OP (MIO) is a bone disorder that occurs in young males and is a public health problem worldwide. However, the detailed pathogenesis of MIO remains to be elucidated. In the present study, blood samples of patients with MIO, senile OP, postmenopausal OP and normal controls (NCs) were obtained for RNA sequencing. Compared with the NC group, differentially expressed genes (DEGs) in the three types of OP were identified. DEGs that were common among the three types of OP and the DEGs that were unique to patients with MIO were determined...
May 7, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29699474/stable-knockdown-of-zbtb7a-promotes-cell-proliferation-and-progression-in-nasopharyngeal-carcinoma
#5
Fei Liu, Fengzhu Tang, Jiao Lan, Wei Jiao, Yongfeng Si, Wensheng Lu, Mingzheng Mo, Bing Li, Jinlong Lu, Jiazhang Wei, Ying Qin, Ruiping Xiao, Benjian Zhang, Yongli Wang, Weiming Xiong
AIMS: Although high expression of ZBTB7A is positively relative to metastasis in nasopharyngeal carcinoma (NPC) patients, the association between its low expression and metastasis of NPC remains unclear. The present study aimed to definitely identify the association. METHODS: The level of ZBTB7A was effectively knocked down by stable transfection of short hair RNA plasmid in NPC cell lines CNE2 and 5-8F (shRNA-CNE2 and shRNA-5-8F), compared with the cells that stably transfected empty plasmid (NC-CNE2 and NC-5-8F)...
January 2018: Tumori
https://www.readbyqxmd.com/read/29683841/monotypic-low-level-hiv-viremias-during-art-are-associated-with-disproportionate-production-of-x4-virions-and-systemic-immune-activation
#6
Marta E Bull, Caroline Mitchell, Jaime Soria, Sheila Styrchak, Corey Williams-Wietzikoski, Jillian Legard, Jennifer Mckernan-Mullin, Kelli Kraft, Frankline Onchiri, Joshua Stern, Sarah Holte, Kevin J Ryan, Edward P Acosta, Alberto La Rosa, Robert W Coombs, Eduardo Ticona, Lisa M Frenkel
OBJECTIVE: During effective antiretroviral therapy (ART), low-level plasma viremias (LLV) (HIV RNA >30-1000 copies/ml) can be detected intermittently. We hypothesized that systemic inflammation is associated with LLV either as the cause or result of the production of virions from clonally expanded cells. METHODS: Prospective cohort study of HIV-infected ART-naïve Peruvians enrolled prior to ART and followed for 2 years. Plasma HIV RNA and peripheral blood mononuclear cell (PBMC) HIV DNA concentrations were quantified pre-ART from individuals whose plasma HIV RNA was ART-suppressed...
April 19, 2018: AIDS
https://www.readbyqxmd.com/read/29679755/long-non-coding-rna-xist-sponges-mir-34a-to-promotes-colon-cancer-progression-via-wnt-%C3%AE-catenin-signaling-pathway
#7
Ningning Sun, Guozun Zhang, Yingying Liu
Little is known about the role of long non-coding RNA XIST in the development of colon cancer. The aim of the present study was to investigate the levels of XIST in colon cancer, and explore its underlying mechanism. In this study, we found XIST expression level was upregulated in colon cancer tissues and cell lines. In addition, the growth rate of cells transfected with si-XIST was significantly decreased compared to that with si-NC, which was reversed by miR-34a targeted with 3'-UTR. Moreover, miR-34a suppressed the expression of WNT1 by binding with the 3'-UTR, which interact with WNT1 to inhibit the proliferation of cells...
April 18, 2018: Gene
https://www.readbyqxmd.com/read/29678912/characterizing-the-ecology-of-vibrio-in-the-neuse-river-estuary-north-carolina-using-heat-shock-protein-60-hsp60-next-generation-amplicon-sequencing
#8
Kelsey J Jesser, Rachel T Noble
Of marine eubacteria, the genus Vibrio is intriguing because member species are relevant to both marine ecology and human health. Many have touted the relationships of Vibrio to environmental factors, especially temperature and salinity, to predict total Vibrio abundance, but these studies lacked the taxonomic resolution to identify relationships among species and key drivers of Vibrio dynamics. In order to improve NGS surveys of Vibrio, we have conducted both 16S small subunit ribosomal RNA (rRNA) and heat shock protein 60 ( hsp60 ) amplicon sequencing of water samples collected at two well-studied locations in the Neuse River Estuary, NC...
April 20, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29673400/long-noncoding-rna-h19-upregulates-vascular-endothelial-growth-factor-a-to-enhance-mesenchymal-stem-cells-survival-and-angiogenic-capacity-by-inhibiting-mir-199a-5p
#9
Jingying Hou, Lingyun Wang, Quanhua Wu, Guanghui Zheng, Huibao Long, Hao Wu, Changqing Zhou, Tianzhu Guo, Tingting Zhong, Lei Wang, Xuxiang Chen, Tong Wang
BACKGROUND: Currently, the overall therapeutic efficiency of mesenchymal stem cells (MSCs) transplantation for the treatment of cardiovascular disease is not satisfactory. The low viability and angiogenic capacity of the implanted cells in the local infarct tissues restrict their further application. Evidence shows that long noncoding RNA H19 (lncRNA-H19) mediates cell survival and angiogenesis. Additionally, it is also involved in MSCs biological activities. This study aimed to explore the functional role of lncRNA-H19 in MSCs survival and angiogenic capacity as well as the underlying mechanism...
April 19, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29668625/lncrna-enst00113-promotes-proliferation-survival-and-migration-by-activating-pi3k-akt-mtor-signaling-pathway-in-atherosclerosis
#10
Xinliang Yao, Chengyun Yan, Lei Zhang, Yanming Li, Qilin Wan
BACKGROUND: Atherosclerosis is one of the most common cardiovascular disorders. The dysfunction of vascular smooth muscle cells (VSMCs) and endothelial cells (ECs) are 2 key factors in the formation of atherosclerosis. This study aims to find strategies to prevent VSMCs and ECs dysfunction for the treatment of atherosclerosis. METHODS: We investigated the expression patterns of long noncoding RNAs (lncRNAs) in 2 pairs of serum samples from both atherosclerosis patients and healthy volunteers through microarray analysis...
April 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29660475/transcriptome-sequencing-of-active-buds-from-populus-deltoides-cl-and-populus-%C3%A3-zhaiguanheibaiyang-reveals-phytohormones-involved-in-branching
#11
Jinnan Wang, Yanting Tian, Jihong Li, Keqiang Yang, Shiyan Xing, Xiaojiao Han, Dong Xu, Yiwei Wang
Branching in woody plants affects their ecological benefits and impacts wood formation. To obtain genome-wide insights into the transcriptome changes and regulatory mechanisms associated with branching, we performed high-throughput RNA sequencing to characterize cDNA libraries generated from active buds of Populus deltoides CL. 'zhonglin2025' (BC) and Populus × zhaiguanheibaiyang (NC). NC has more branches than BC and rapid growth. We obtained a total of 198.2 million high-quality clean reads from the NC and BC libraries...
April 13, 2018: Genomics
https://www.readbyqxmd.com/read/29644261/detection-and-analysis-of-circular-rnas-by-rt-pcr
#12
Amaresh C Panda, Myriam Gorospe
Gene expression in eukaryotic cells is tightly regulated at the transcriptional and posttranscriptional levels. Posttranscriptional processes, including pre-mRNA splicing, mRNA export, mRNA turnover, and mRNA translation, are controlled by RNA-binding proteins (RBPs) and noncoding (nc)RNAs. The vast family of ncRNAs comprises diverse regulatory RNAs, such as microRNAs and long noncoding (lnc)RNAs, but also the poorly explored class of circular (circ)RNAs. Although first discovered more than three decades ago by electron microscopy, only the advent of high-throughput RNA-sequencing (RNA-seq) and the development of innovative bioinformatic pipelines have begun to allow the systematic identification of circRNAs (Szabo and Salzman, 2016; Panda et al ...
March 20, 2018: Bio-protocol
https://www.readbyqxmd.com/read/29627834/effects-of-long-non-coding-rna-linc00963-on-renal-interstitial-fibrosis-and-oxidative-stress-of-rats-with-chronic-renal-failure-via-the-foxo-signaling-pathway
#13
Wen Chen, Lei Zhang, Zhong-Qi Zhou, Yue-Qin Ren, Li-Na Sun, Yu-Lin Man, Zhong-Wei Ma, Zhi-Kui Wang
BACKGROUND/AIMS: Chronic renal failure (CRF) is usually associated with chronic diseases such as congestive heart failure and diabetes mellitus, the prevalence of which is increased with age. This study is designed to investigate the role of long intergenic non-coding RNA (lincRNA) LINC00963 in renal interstitial fibrosis (RIF) and oxidative stress (OS) of CRF via the forkhead box O (FoxO) signaling pathway. METHODS: Microarray data and annotated probe files related to CRF were downloaded by retrieving Gene Expression Omnibus (GEO) database to screen differentially expressed lncRNA...
March 29, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29600544/down-regulated-long-non-coding-rna-anril-restores-the-learning-and-memory-abilities-and-rescues-hippocampal-pyramidal-neurons-from-apoptosis-in-streptozotocin-induced-diabetic-rats-via-the-nf-%C3%AE%C2%BAb-signaling-pathway
#14
Xin Wen, Xin-Rui Han, Yong-Jian Wang, Shan Wang, Min Shen, Zi-Feng Zhang, Shao-Hua Fan, Qun Shan, Liang Wang, Meng-Qiu Li, Bin Hu, Chun-Hui Sun, Dong-Mei Wu, Jun Lu, Yuan-Lin Zheng
Diabetes often causes learning and memory deficits, which leads to unfavorable behavioral performance. In this study, we investigated the effects of long non-coding RNA (lncRNA) ANRIL on learning, memory abilities, and hippocampal neuronal apoptosis via the NF-κB signaling pathway in streptozotocin (STZ)-induced diabetic rats. After successful establishment of diabetic rat models, the subjects were then assigned into the DM, DM + si-ANRIL, DM + si-negative control (si-NC) groups, as well as an additional normal group...
March 30, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29593432/downregulation-of-hsa_circ_0011946-suppresses-the-migration-and-invasion-of-the-breast-cancer-cell-line-mcf-7-by-targeting-rfc3
#15
Juan Zhou, Wen-Wen Zhang, Fang Peng, Jia-Yuan Sun, Zhen-Yu He, San-Gang Wu
Introduction: Although some circRNAs have been found to regulate the progression of malignancies, their functions and coupled molecular mechanisms are still unclear. In our study, we sought to assess the underlying molecular mechanisms of circRNAs in breast cancer and therefore explored the differentially expressed circRNAs and co-expression networks, followed by in vitro experiments. Materials and methods: High-throughput RNA sequencing was performed to obtain an unbiased profile of circRNA expression...
2018: Cancer Management and Research
https://www.readbyqxmd.com/read/29580971/retroviral-gag-protein-rna-interactions-implications-for-specific-genomic-rna-packaging-and-virion-assembly
#16
REVIEW
Erik D Olson, Karin Musier-Forsyth
Retroviral Gag proteins are responsible for coordinating many aspects of virion assembly. Gag possesses two distinct nucleic acid binding domains, matrix (MA) and nucleocapsid (NC). One of the critical functions of Gag is to specifically recognize, bind, and package the retroviral genomic RNA (gRNA) into assembling virions. Gag interactions with cellular RNAs have also been shown to regulate aspects of assembly. Recent results have shed light on the role of MA and NC domain interactions with nucleic acids, and how they jointly function to ensure packaging of the retroviral gRNA...
March 23, 2018: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/29572071/long-non-coding-rna-hotair-and-stat3-synergistically-regulate-the-cervical-cancer-cell-migration-and-invasion
#17
Yan Zhang, Xiaoling Cheng, Hua Liang, Zhenzhen Jin
Homeobox (HOX) transcript antisense RNA (HOTAIR) is a long intergenic non-coding RNA (lncRNA) that has been reported to be highly upregulated in several types of cancers. However, the role of HOTAIR in human cervical cancer is still unclear. We therefore investigated the expression and probable function of HOTAIR in cervical cancer cells. The expression of HOTAIR was examined in (HeLa, CaSki, ME-180, HT-3) and Human Cervical Epithelial Cells (HCerEpiC) by qRT-PCR. Transfection of si-NC, si-HOTAIR or si-STAT3 was carried out with the help of Lipofectamine 2000...
March 20, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29571744/the-nc-domain-of-hiv-1-gag-contributes-to-the-interaction-of-gag-with-tsg101
#18
Salah Edin El Meshri, Emmanuel Boutant, Assia Mouhand, Audrey Thomas, Valéry Larue, Ludovic Richert, Valérie Vivet-Boudou, Yves Mély, Carine Tisné, Delphine Muriaux, Hugues de Rocquigny
BACKGROUND: HIV-1 Gag polyprotein orchestrates the assembly of viral particles. Its C-terminus consists of the nucleocapsid (NC) domain that interacts with RNA, and the p6 domain containing the PTAP motif that binds the cellular ESCRT factor TSG101 and ALIX. Deletion of the NC domain of Gag (GagNC) results in defective Gag assembly, a decrease in virus production and, thus probably affects recruitment of the ESCRT machinery. To investigate the role of GagNC in this recruitment, we analysed its impact on TSG101 and ALIX localisations and interactions in cells expressing Gag...
March 20, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29568943/identification-of-aberrantly-expressed-long-non-coding-rnas-in-postmenopausal-osteoporosis
#19
Qi Fei, Xiaodong Bai, Jisheng Lin, Hai Meng, Yong Yang, Ai Guo
Postmenopausal osteoporosis (PMOP) is a common skeletal disorder in postmenopausal women. The present study aimed to identify the key long non‑coding RNAs (lncRNAs) in PMOP through RNA sequencing. RNA sequencing was performed to obtain the expression profile of lncRNAs and mRNAs in blood samples of patients with PMOP and normal controls (NCs). Following the identification of differentially expressed mRNAs (DEmRNAs) and differentially expressed lncRNAs (DElncRNAs), the DElncRNA-DEmRNA co‑expression network was constructed...
March 19, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29565488/long-noncoding-rna-lnc01614-promotes-the-occurrence-and-development-of-gastric-cancer-by-activating-emt-pathway
#20
Y Dong, Z-G Wang, T-S Chi
OBJECTIVE: To investigate the expression characteristics of long noncoding ribonucleic acid (lncRNA) lnc01614 in gastric cancer, so as to further investigate its roles in the occurrence and development of gastric cancer and its potential regulatory mechanism. PATIENTS AND METHODS: Quantitative Real-time polymerase chain reaction (qRT-PCR) was used to detect the expression levels of lnc01614 in 79 pairs of gastric cancer tissues and normal adjacent tissues. The correlations of lnc01614 expression with pathological parameters of gastric cancer and prognoses of patients were also analyzed...
March 2018: European Review for Medical and Pharmacological Sciences
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