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https://www.readbyqxmd.com/read/28718958/multiplex-pcr-scheme-for-variant-plasmid-mediated-class-c-%C3%AE-lactamase-typing
#1
Le Van Chuong, Virapong Prachayasittikul, Chartchalerm Isarankura Na Ayudhya, Ratana Lawung
BACKGROUND: An increasing of prevalence and diversification of plasmid-mediated AmpC (pAmpC) has been emerged worldwide. The incidence of pAmpC resulted in increasing β-lactamase production and conferred resistance to almost all β-lactam antibiotics excluding carbapenems. The lack of standard method for pAmpC identification and classification exert a challenge in epidemiological surveillance and infection control practices. METHODS: A robust, single tube multiplex PCR has been developed to classify six different pAmpC groups including CIT (CMY-2 like, LAT and CFE), ECB (ACT, MIR), MOX & CMY-1 like, DHA, ACC, and FOX...
July 18, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28678264/self-assembled-nanocapsules-in-water-a-molecular-mechanistic-study
#2
Hang Xiao, Xiaoyang Shi, Xi Chen
The self-assembly mechanism of one-end-open carbon nanotubes (CNTs) suspended in an aqueous solution was studied by molecular dynamics simulations. It was shown that two one-end-open CNTs with different diameters can coaxially self-assemble into a nanocapsule. The nanocapsules formed were stable in aqueous solution under ambient conditions, and the pressure inside the nanocapsule was much higher than the ambient pressure due to the van der Waals interactions between the two parts of the nanocapsule. The effects of the normalized radius difference, normalized inter-tube distance and aspect ratio of the CNT pairs were systematically explored...
July 5, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28650030/carbon-nanoscroll-silk-crystallite-hybrid-structures-with-controllable-hydration-and-mechanical-properties
#3
Yuan Cheng, Leng-Duei Koh, Fan Wang, Dechang Li, Baohua Ji, Jingjie Yeo, Guijian Guan, Ming-Yong Han, Yong-Wei Zhang
Hybrid structures of nanomaterials (e.g. tubes, scrolls, threads, cages) and biomaterials (e.g. proteins) hold tremendous potential for applications as drug carriers, biosensors, tissue scaffolds, cancer therapeutic agents, etc. However, in many cases, the interacting forces at the nano-bio interfaces and their roles in controlling the structures and dynamics of nano-bio-hybrid systems are very complicated but poorly understood. In this study, we investigate the structure and mechanical behavior of a protein-based hybrid structure, i...
July 6, 2017: Nanoscale
https://www.readbyqxmd.com/read/28574076/bushy-sphere-dendrites-with-husk-shaped-branches-axially-spreading-out-from-the-core-for-photo-catalytic-oxidation-remediation-of-toxins
#4
Mohamed A Shenashen, Satoshi Kawada, Mahmoud M Selim, Wafaa M Morsy, Hitoshi Yamaguchi, Abdulaziz A Alhamid, Naoki Ohashi, Izumi Ichinose, Sherif A El-Safty
This work describes densely interlinked bushy "tree-like chains" characterized by neatly branched sphere dendrites (bushy sphere dendrites, BSD) with long fan-like, husk-shaped branching paths that extend longitudinally from the core axis of the {110}-exposed plane. We confirmed that the hierarchical dendrite surfaces created bowls of swirled caves along the tree-tube in the mat-like branches. These surfaces had high-index catalytic site facets associated with the formation of ridges/defects on the dominant {110}-top-cover surface...
June 2, 2017: Nanoscale
https://www.readbyqxmd.com/read/28574066/chirality-dependent-growth-of-single-wall-carbon-nanotubes-as-revealed-inside-nano-test-tubes
#5
Marianna V Kharlamova, Christian Kramberger, Takeshi Saito, Yuta Sato, Kazu Suenaga, Thomas Pichler, Hidetsugu Shiozawa
Growth dynamics of single-wall carbon nanotubes (SWCNTs) have been studied with nickelocene as a precursor encapsulated in the interior of template SWCNTs. By means of multi-laser Raman spectroscopy, growth curves of nine different SWCNTs, (8,8), (12,3), (13,1), (9,6), (10,4), (11,2), (11,1), (9,3) and (9,2), have been determined upon in situ annealing at various temperatures. The data reveal that the nanotubes grow through fast and slow reaction pathways with high and low activation energies, respectively...
June 2, 2017: Nanoscale
https://www.readbyqxmd.com/read/28558429/workplace-exposure-and-release-of-ultrafine-particles-during-atmospheric-plasma-spraying-in-the-ceramic-industry
#6
M Viana, A S Fonseca, X Querol, A López-Lilao, P Carpio, A Salmatonidis, E Monfort
Atmospheric plasma spraying (APS) is a frequently used technique to produce enhanced-property coatings for different materials in the ceramic industry. This work aimed to characterise and quantify the impact of APS on workplace exposure to airborne particles, with a focus on ultrafine particles (UFPs, <100nm) and nanoparticles (<50nm). Particle number, mass concentrations, alveolar lung deposited surface area concentration, and size distributions, in the range 10nm-20μm were simultaneously monitored at the emission source, in the potential worker breathing zone, and in outdoor air...
December 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28531985/enhancement-of-electro-chemical-properties-of-tio2-nanotubes-for-biological-interfacing
#7
D Khudhair, H Amani Hamedani, J Gaburro, S Shafei, S Nahavandi, H Garmestani, A Bhatti
Micro/nano electrodes employing nanotubes has attracted paramount attention in recent years due to their inherent superior mechanical and structural properties. Electrical interfaces with different geometries and sizes have been developed as electrodes for measuring action potentials and investigating neural information processing in neural networks. In this work, we investigated the possibility of using TiO2 nanotube arrays that were grown using electrochemical anodization technique, as a micro/nano electrode for neural interfacing...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531859/the-role-of-conductive-materials-in-the-start-up-period-of-thermophilic-anaerobic-system
#8
Wangwang Yan, Nan Shen, Yeyuan Xiao, Yun Chen, Faqian Sun, Vinay Kumar Tyagi, Yan Zhou
The major obstacle for thermophilic anaerobic digestion (TAD) is the inhibited microorganism activity and process instability during the start-up period. This study proposed a strategy to accelerate and stabilize the thermophilic reactors start-up via adding conductive materials. The results show that methane production rate in conductive materials supplemented (CMS) reactors was almost two times higher than the control reactors. Caloramator sp., a candidate of electroactive bacteria, was significantly enriched in the carbon nano-tube (CNT) supplemented groups (12...
September 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28522411/halloysite-and-chitosan-oligosaccharide-nanocomposite-for-wound-healing
#9
Giuseppina Sandri, Carola Aguzzi, Silvia Rossi, Maria Cristina Bonferoni, Giovanna Bruni, Cinzia Boselli, Antonia Icaro Cornaglia, Federica Riva, Cesar Viseras, Carla Caramella, Franca Ferrari
Halloysite is a natural nanotubular clay mineral (HNTs, Halloysite Nano Tubes) chemically identical to kaolinite and, due to its good biocompatibility, is an attractive nanomaterial for a vast range of biological applications. Chitosan oligosaccharides are homo- or heterooligomers of N-acetylglucosamine and D-glucosamine, that accelerate wound healing by enhancing the functions of inflammatory and repairing cells. The aim of the work was the development of a nanocomposite based on HNTs and chitosan oligosaccharides, to be used as pour powder to enhance healing in the treatment of chronic wounds...
May 15, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28504151/the-fabrication-of-microcrystalline-cellulose-nanozno-hybrid-composites-and-their-application-in-rubber-compounds
#10
Yunhao Liang, Xiaoling Liu, Lili Wang, Jutao Sun
Microcrystalline cellulose (MCC) was first swollen and then hybridized with nano-ZnO to prepare MCC-ZnO hybrid composites using a microreactor technique. The microstructure of the ZnO particles was controlled and characterized. The results showed that the nano-ZnO particles had a Wurtzite structure and were successfully loaded on the surface of the MCC, and the ZnO morphologies could be shaped as spheres, rods or tubes by controlling the size of microreactor. The hybrid ratio of ZnO was approximately 20%. The MCC-ZnO hybrids were used in SSBR2557A/SiO2 compounds to replace portions of the silica...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28496207/selective-doping-of-ni-2-in-highly-transparent-glass-ceramics-containing-nano-spinels-znga2o4-and-zn1-x-ga2-2x-ge-x-o4-for-broadband-near-infrared-fiber-amplifiers
#11
Zhigang Gao, Yinyao Liu, Jing Ren, Zaijin Fang, Xiaosong Lu, Elfed Lewis, Gerald Farrell, Jun Yang, Pengfei Wang
Selective doping of Ni(2+) in octahedral sites provided by nanocrystals embedded in glass-ceramics (GCs) is crucial to the enhancement of broadband near-infrared (NIR) emission. In this work, a NIR emission with a full-width-at-half-maximum (FWHM) of 288 nm is first reported from ZnGa2O4: Ni(2+) nano-spinels embedded GCs with excellent transparency. A comparison is made of the NIR luminescence properties of Ni(2+) doped GCs containing ZnGa2O4, germanium-substituted ZnGa2O4 nano-spinels (Zn1+x Ga2-2x Ge x O4), and Zn2GeO4/Li2Ge4O9 composite nanocrystals that are free of Ga(3+)...
May 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28482479/a-novel-and-sensitive-hexadecyltrimethylammoniumbromide-functionalized-fe-decorated-mwcnts-modified-carbon-paste-electrode-for-the-selective-determination-of-quercetin
#12
Veera Erady, Ronald J Mascarenhas, Ashis K Satpati, Simon Detriche, Zineb Mekhalif, Joseph Delhalle, A Dhason
The fabrication of differential pulse voltametry (DPV) sensor for the effective detection of Quercetin (QR) was achieved by modifying carbon paste electrode (CPE) with Iron decorated multi walled carbon nano tubes (Fe-MWCNTs) followed by drop casting of hexadecyltrimethylammonium bromide onto the surface for optimal results. Cyclic voltammetry and DPV techniques were used for qualitative and quantitative analysis of QR (Quercetin) respectively. The sensor revealed impressive electro-catalytic behavior towards oxidation of QR with almost 6...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28470859/zirconium-oxide-and-niobium-oxide-used-as-radiopacifiers-in-a-calcium-silicate-based-material-stimulate-fibroblast-proliferation-and-collagen-formation
#13
G F Silva, J M Guerreiro-Tanomaru, T S da Fonseca, M I B Bernardi, E Sasso-Cerri, M Tanomaru-Filho, P S Cerri
AIM: To evaluate the influence of the addition of microparticulate (micro) and nanoparticulate (nano) zirconium oxide (ZrO2 ) and niobium pentoxide (Nb2 O5 ) to a calcium silicate-based cement (CS) on the subcutaneous healing process in rats compared with MTA Angelus™. METHODOLOGY: In each rat, two polyethylene tubes filled with the following materials: (i) MTA; (ii) CS + ZrO2 micro; (iii) CS + ZrO2 nano; (iv) CS + Nb2 O5 micro or (v) CS + Nb2 O5 nano were implanted subcutaneously; empty polyethylene tubes were used in the Control group...
May 4, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28455219/ph-sensitive-polymeric-nanoparticles-for-co-delivery-of-doxorubicin-and-curcumin-to-treat-cancer-via-enhanced-pro-apoptotic-and-anti-angiogenic-activities
#14
Jinming Zhang, Jingjing Li, Zhi Shi, Yang Yang, Xi Xie, Simon MingYuen Lee, Yitao Wang, Kam W Leong, Meiwan Chen
Co-delivery of multiple drugs with complementary anticancer mechanisms by nano-carriers offers an effective strategy to treat cancer. The combination of drugs with pro-apoptotic and anti-angiogenic activities is potentially effective in treating human hepatocellular carcinoma (HCC). Herein, we developed a co-delivery system for doxorubicin (Dox), a pro-apoptotic drug, and curcumin (Cur), a potent drug for antiangiogenesis, in pH-sensitive nanoparticles (NPs) constituted with amphiphilic poly(β-amino ester) copolymer...
April 25, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28448120/structural-properties-of-double-walled-carbon-nanotubes-driven-by-mechanical-interlayer-coupling
#15
Ahmed Ghedjatti, Yann Magnin, Frédéric Fossard, Guillaume Wang, Hakim Amara, Emmanuel Flahaut, Jean-Sébastien Lauret, Annick Loiseau
Structural identification of double-walled carbon nanotubes (DWNTs) is presented through a robust procedure based on the latest generation of transmission electron microscope, making possible a statistical analysis based on numerous nano-objects. This approach reveals that inner and outer tubes of DWNTs are not randomly oriented, suggesting the existence of a mechanical coupling between the two concentric walls. With the support of atomic-scale modeling, we attribute it to the presence of incommensurate domains whose structures depend on the diameters and helicities of both tubes and where inner tubes try to achieve a local stacking orientation to reduce strain effects...
May 3, 2017: ACS Nano
https://www.readbyqxmd.com/read/28444473/contrasting-effects-of-engineered-carbon-nanotubes-on-plants-a-review
#16
Meththika Vithanage, Mihiri Seneviratne, Mahtab Ahmad, Binoy Sarkar, Yong Sik Ok
Rapid surge of interest for carbon nanotube (CNT) in the last decade has made it an imperative member of nanomaterial family. Because of the distinctive physicochemical properties, CNTs are widely used in a number of scientific applications including plant sciences. This review mainly describes the role of CNT in plant sciences. Contradictory effects of CNT on plants physiology are reported. CNT can act as plant growth inducer causing enhanced plant dry biomass and root/shoot lengths. At the same time, CNT can cause negative effects on plants by forming reactive oxygen species in plant tissues, consequently leading to cell death...
April 25, 2017: Environmental Geochemistry and Health
https://www.readbyqxmd.com/read/28427648/sonochemical-synthesis-characterization-and-effects-of-temperature-power-ultrasound-and-reaction-time-on-the-morphological-properties-of-two-new-nanostructured-mercury-ii-coordination-supramolecule-compounds
#17
Payam Hayati, Ali Reza Rezvani, Ali Morsali, Daniel Ruiz Molina, Samira Geravand, Salvio Suarez-Garcia, Miguel Angel Moreno Villaecija, S García-Granda, Rafael Mendoza-Meroño, Pascal Retailleau
Two new mercury(II) coordination supramolecular compounds (CSCs) (1D and 0D), [Hg(L)(I)2]n (1) and [Hg2(L')2(SCN)2]·2H2O (2) (L=2-amino-4-methylpyridine and L'=2,6-pyridinedicarboxlic acid), have been synthesized under different experimental conditions. Micrometric crystals (bulk) or nano-sized materials have been obtained depending on using the branch tube method or sonochemical irradiation. All materials have been characterized by field emission scanning electron microscope (FESEM), scanning electron microscopy (SEM), powder X-ray diffraction (PXRD) and FT-IR spectroscopy...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28398143/a-theoretical-study-on-the-stability-of-cnt-encased-cyclic-peptide-beyond-hydrogen-bond-cut-off
#18
Subramanian Vidhyasankar, Nallasamy Dharmaraj, Ponmalai Kolandaivel
The Carbon nanotubes (CNT) are potential candidate for many biomedical applications especially in targeted drug delivery for cancer diseases. However, the use of CNT has limitations due to its insolubility in aqueous media. The self-assembly of cyclic peptide encased on the CNT has enhanced its dispersion in aqueous medium which extend their applications as antibacterial and drug delivery agents. To understand this process, an attempt has been made to investigate the dynamics and stability of trimer cyclic peptide encasing with CNT using classical molecular dynamics...
April 16, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28385266/self-packed-core-shell-nano-liquid-chromatography-columns-and-silica-based-monolithic-trap-columns-for-targeted-proteomics
#19
Henriette Sjaanes Berg, Kristina Erikstad Seterdal, Tone Smetop, Rūdolfs Rozenvalds, Ole Kristian Brandtzaeg, Tore Vehus, Elsa Lundanes, Steven Ray Wilson
Self-preparation of nano liquid chromatography (nLC) columns has advantages regarding cost and flexibility. For targeted proteomics, we evaluated several approaches for particle-packing nLC columns and manufacturing fritless silica-based monolithic trap columns (50μm inner diameter). Our preferred approach for nLC column preparation was to magnetically stir Accucore core shell particles (C18 stationary phase) in ACN/water (80/20, v/v) suspensions during pressure-driven filling of polymer-fritted standard fused silica capillaries...
March 21, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28385265/microfluidic-high-performance-liquid-chromatography-chip-hyphenation-to-inductively-coupled-plasma-mass-spectrometry
#20
David P Bishop, Lucas Blanes, Alexander B Wilson, Thor Wilbanks, Kevin Killeen, Rudolf Grimm, Ross Wenzel, Derek Major, Mirek Macka, David Clarke, Robin Schmid, Nerida Cole, Philip A Doble
The Agilent Chip Cube Interface is a microfluidic chip-based technology originally designed for nanospray molecular mass spectrometry in which the sample enrichment, nano-column, tubing, connectors and spray tip were integrated into a single biocompatible chip. Here we describe the hyphenation of the Chip Cube Interface to ICP-MS via modification of the standard HPLC chip design and a new total consumption nebuliser suitable for flow rates as low as 300nLmin(-1). The potential of the instrument to eliminate common nanoLC - ICP-MS shortcomings such as leaks, blockages and band-broadening was demonstrated via analysis of cyanocobalamin in equine plasma...
May 12, 2017: Journal of Chromatography. A
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