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caveolin-1

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https://www.readbyqxmd.com/read/28806774/fibroblast-growth-factor-receptor-1-mediates-internalization-of-pathogenic-spotted-fever-rickettsiae-into-host-endothelium
#1
Abha Sahni, Jignesh Patel, Hema P Narra, Casey L C Schroeder, David H Walker, Sanjeev K Sahni
Rickettsial infections continue to cause serious morbidity and mortality in severe human cases around the world. Host cell adhesion and invasion is an essential requisite for intracellular growth, replication, and subsequent dissemination of pathogenic rickettsiae. Heparan sulfate proteoglycans [HSPGs] facilitate the interactions between fibroblast growth factor(s) and their tyrosine kinase receptors resulting in receptor dimerization/activation and have been implicated in bacterial adhesion to target host cells...
2017: PloS One
https://www.readbyqxmd.com/read/28805811/caveolin-1-dependent-nanoscale-organization-of-the-bcr-regulates-b-cell-tolerance
#2
Susana Minguet, Kathrin Kläsener, Anna-Maria Schaffer, Gina J Fiala, Teresa Osteso-Ibánez, Katrin Raute, Inmaculada Navarro-Lérida, Frederike A Hartl, Maximilian Seidl, Michael Reth, Miguel A Del Pozo
Caveolin-1 (Cav1) regulates the nanoscale organization and compartmentalization of the plasma membrane. Here we found that Cav1 controlled the distribution of nanoclusters of isotype-specific B cell antigen receptors (BCRs) on the surface of B cells. In mature B cells stimulated with antigen, the immunoglobulin M BCR (IgM-BCR) gained access to lipid domains enriched for GM1 glycolipids, by a process that was dependent on the phosphorylation of Cav1 by the Src family of kinases. Antigen-induced reorganization of nanoclusters of IgM-BCRs and IgD-BCRs regulated BCR signaling in vivo...
August 14, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28798329/measurement-of-caveolin-1-densities-in-the-cell-membrane-for-quantification-of-caveolar-deformation-after-exposure-to-hypotonic-membrane-tension
#3
Masashi Tachikawa, Nobuhiro Morone, Yosuke Senju, Tadao Sugiura, Kyoko Hanawa-Suetsugu, Atsushi Mochizuki, Shiro Suetsugu
Caveolae are abundant flask-shaped invaginations of plasma membranes that buffer membrane tension through their deformation. Few quantitative studies on the deformation of caveolae have been reported. Each caveola contains approximately 150 caveolin-1 proteins. In this study, we estimated the extent of caveolar deformation by measuring the density of caveolin-1 projected onto a two-dimensional (2D) plane. The caveolin-1 in a flattened caveola is assumed to have approximately one-quarter of the density of the caveolin-1 in a flask-shaped caveola...
August 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28791417/diverse-gene-expression-patterns-in-response-to-anticancer-drugs-between-human-and-mouse-cell-lines-revealed-by-a-comparative-transcriptomic-analysis
#4
Yong Guo, Zhuoran Liang, Xiaoliang Hou, Zhi Zhang
The aim of the present study was to perform comparative genomics using gene expression profile datasets of mice and humans who had been treated with anticancer drugs, to determine the similarities and differences in the antitumor mechanisms in the two mammals. This involved data mining of antitumor gene expression regulation, and screening of genetic loci from experimental mouse models of antitumor targets, to provide a theoretical basis of drug design. Subsequently, 9 overlapping genes with opposite expression patterns were identified across mouse and human cell lines that were treated with a specific cyclin‑dependent kinase 4/6 inhibitor, PD0332991...
August 4, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28789920/the-%C3%AF-carboxyl-group-of-7-ketocholesteryl-9-carboxynonanoate-mediates-the-binding-of-oxldl-to-cd36-receptor-and-enhances-caveolin-1-expression-in-macrophages
#5
Jingda Li, Chengjie Yu, Renjun Wang, Jianrong Xu, Yan Chi, Jianzhong Qin, Qingping Liu
CD36 signal transduction modulates the uptake of oxidized low-density lipoprotein (oxLDL) and foam cell formation. We previously observed that 7-ketocholesteryl-9-carboxynonanoate (oxLig-1), the lipid moiety of oxLDL, activates the CD36-Src-JNK/ERK1/2 signalling pathway. In this study, we assessed the role of the ω-carboxyl group in the binding of oxLig-1 to CD36 and investigated whether the binding of the ω-carboxyl group to CD36 triggers CD36-mediated signalling, thereby resulting in the upregulation of caveolin-1 expression...
August 5, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28778116/caveolin-1-restricts-group-a-streptococcus-invasion-of-non-phagocytic-host-cells
#6
Jin Yan Lim, Timothy C Barnett, Michele Bastiani, Kerrie-Ann McMahon, Charles Ferguson, Richard I Webb, Robert G Parton, Mark J Walker
Caveolae are composed of two major proteins, caveolin-1 (CAV1) and cavin-1/polymerase transcript factor I (CAVIN1). Here we demonstrate that CAV1 levels modulate invasion of group A Streptococcus (GAS) into non-phagocytic mammalian cells. GAS showed enhanced internalization into CAV1 knockout mouse embryonic fibroblasts (MEFs), and CAV1 knockdown human epithelial HEp-2 cells, whereas over-expression of CAV1 in HEp-2 cells reduced GAS invasion. This effect was not dependent on the expression of the GAS fibronectin binding protein SfbI, which had previously been implicated in caveolae-mediated uptake...
August 4, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28766344/cell-shaping-micropatterns-for-quantitative-super-resolution-microscopy-imaging-of-membrane-mechanosensing-proteins
#7
Anthony Fernandez, Markville Bautista, Ramunas Stanciauskas, Taerin Chung, Fabien Pinaud
Patterning cells on microcontact-printed substrates is a powerful approach to control cell morphology and introduce specific mechanical cues on a cell's molecular organization. Although global changes in cellular architectures caused by micropatterns can easily be probed with diffraction-limited optical microscopy, studying molecular reorganizations at the nanoscale demands micropatterned substrates that accommodate the optical requirements of single molecule microscopy techniques. Here, we developed a simple micropatterning strategy that provides control of cellular architectures and is optimized for nanometer accuracy single molecule tracking and three-dimensional super-resolution imaging of plasma and nuclear membrane proteins in cells...
August 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28760776/tie2-associates-with-caveolae-and-regulates-caveolin-1-to-promote-their-nuclear-translocation
#8
Mohammad B Hossain, Rehnuma Shifat, Jingyi Li, Xuemei Luo, Kenneth R Hess, Yisel Rivera-Molina, Francisco P Martinez, Hong Jiang, Frederick F Lang, Mien-Chie Hung, Juan Fueyo, Candelaria Gomez-Manzano
DNA repair pathways are aberrant in cancer, enabling tumor cells to survive standard therapies-chemotherapy and radiotherapy. Our group previously reported that, upon irradiation, the membrane-bound tyrosine kinase receptor TIE2 translocates into the nucleus and phosphorylates histone H4 at Tyr51, recruiting ABL1 to the DNA repair complexes that participate in the non-homologous end-joining pathway. However, no specific molecular mechanisms of TIE2 endocytosis have been reported. Here, we show that irradiation or ligand-induced TIE2 trafficking is dependent on caveolin-1, the main component of caveolae...
July 31, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28758625/the-dengue-virus-non-structural-protein-1-ns1-is-secreted-from-infected-mosquito-cells-via-a-non-classical-caveolin-1-dependent-pathway
#9
Ana C Alcalá, Raiza Hernández-Bravo, Fernando Medina, David S Coll, Jose L Zambrano, Rosa M Del Angel, Juan E Ludert
Dengue virus NS1 is a glycoprotein of 46-50 kDa that is conserved among flaviviruses, associates as a dimer to cell membranes and is secreted as a hexamer to the extracellular milieu. Recent evidence showed that NS1 is secreted efficiently from infected mosquito cells. To explore the secretory route of NS1 in mosquito cells, infected cells were treated with brefeldin A (BFA) and methyl-beta-cyclodextrin (MβCD). The results showed that MβCD, but not BFA, significantly reduced the release of NS1. Moreover, silencing the expression of caveolin-1 (CAV1; a key component of the caveolar system that transports cholesterol inside the cell), but not SAR1 (a GTPase that participates in the classical secretory pathway), also results in a significant reduction of the secretion of NS1...
July 31, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28754863/pathological-expression-of-tissue-factor-confers-promising-antitumor-response-to-a-novel-therapeutic-antibody-sc1-in-triple-negative-breast-cancer-and-pancreatic-adenocarcinoma
#10
Xuesai Zhang, Qingrou Li, Hui Zhao, Lanping Ma, Tao Meng, Jianchang Qian, Rui Jin, Jingkang Shen, Ker Yu
The pathological presence of tissue factor (TF) in cancer cells promotes tumor-initiated thrombosis and cancer metastasis. We found that TF is aberrantly present in large percentage of aggressive triple negative breast cancer (TNBC) and pancreatic adenocarcinoma (PaC), two most lethal forms of malignancy that urgently need effective treatment. TF expression in TNBC clustered with higher levels of vimentin, basal-type keratins KRT5/14 and caveolin-1 but lower levels of luminal-type biomarkers. We developed a novel and specific anti-TF therapeutic antibody SC1, which displayed an exceedingly high potency against TF extracellular domain (EC50: 0...
July 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28753840/caveolin-1-expression-in-upper-tract-urothelial-carcinoma
#11
David D'Andrea, Marco Moschini, Beat Foerster, Mohammad Abufaraj, Vitaly Margulis, Jose Karam, Yair Lotan, Jay Raman, Romain Mathieu, Morgan Rouprêt, Pierre I Karakiewicz, Alberto Briganti, Andrea Haitel, Sharhrokh F Shariat
BACKGROUND: Improvement in postoperative risk stratification of upper tract urothelial carcinoma (UTUC) is required to better predict outcomes and counsel patients on adjuvant treatment. OBJECTIVE: To validate the association between caveolin-1 and oncological outcomes in patients treated with radical nephroureterectomy (RNU) for UTUC. DESIGN, SETTING, AND PARTICIPANTS: Caveolin-1 expression was evaluated via immunochemistry on a tissue microarray from 621 patients...
July 1, 2017: European Urology Focus
https://www.readbyqxmd.com/read/28753584/characterization-of-physiochemical-properties-of-caveolin-1-from-normal-and-prion-infected-human-brains
#12
Xiangzhu Xiao, Pingping Shen, Zerui Wang, Johnny Dang, Alise Adornato, Lewis S Zou, Zhiqian Dong, Jue Yuan, Jiachun Feng, Li Cui, Wen-Quan Zou
Caveolin-1 is a major component protein of the caveolae-a type of flask shaped, 50-100 nm, nonclathrin-coated, microdomain present in the plasma membrane of most mammalian cells. Caveolin-1 functions as a scaffolding protein to organize and concentrate signaling molecules within the caveolae, which may be associated with its unique physicochemical properties including oligomerization, acquisition of detergent insolubility, and association with cholesterol. Here we demonstrate that caveolin-1 is detected in all brain areas examined and recovered in both detergent-soluble and -insoluble fractions...
July 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/28751541/downregulation-of-cavin-1-expression-via-increasing-caveolin-1-degradation-prompts-the-proliferation-and-migration-of-vascular-smooth-muscle-cells-in-balloon-injury-induced-neointimal-hyperplasia
#13
Li-Jun Zhou, Xue-Ying Chen, Shui-Ping Liu, Lin-Lin Zhang, Ya-Nan Xu, Pan-Wei Mu, Deng-Feng Geng, Zhi Tan
BACKGROUND: Percutaneous coronary intervention has been widely used in the treatment of ischemic heart disease, but vascular restenosis is a main limitation of percutaneous coronary intervention. Our previous work reported that caveolin-1 had a key functional role in intimal hyperplasia, whereas whether Cavin-1 (another important caveolae-related protein) was involved is still unknown. Therefore, we will investigate the effect of Cavin-1 on neointimal formation. METHODS AND RESULTS: Balloon injury markedly reduced Cavin-1 protein and enhanced ubiquitin protein expression accompanied with neointimal hyperplasia in injured carotid arteries, whereas Cavin-1 mRNA had no change...
July 27, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28747549/inhibitory-effect-of-caveolin-1-in-vascular-endothelial-cells-pericytes-and-smooth-muscle-cells
#14
Hongping Xu, Liwei Zhang, Wei Chen, Jiazhou Xu, Ruting Zhang, Ran Liu, Lan Zhou, Wenjie Hu, Rong Ju, Chunsik Lee, Weisi Lu, Anil Kumar, Xuri Li, Zhongshu Tang
Caveolin-1 (Cav1) is the principle structural protein of caveolae. It plays important roles in the vascular system under both physiological and pathological conditions. Although Cav1 has been shown to inhibit microvascular permeability and has been considered as a tumor-suppressor for years, the underlying cellular mechanism has yet to be discovered. Here, we systematically investigated Cav1 functions in the main types of vascular cells, including endothelial cells (ECs), pericytes (PCs) and smooth muscle cells (SMCs)...
July 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/28747506/hcv-induces-the-localization-of-lipid-rafts-to-autophagosomes-for-its-rna-replication
#15
Ja Yeon Kim, Linya Wang, Jiyoung Lee, Jing-Hsiung James Ou
Autophagy plays important roles in maintaining cellular homeostasis. It uses double- or multiple- membrane vesicles termed autophagosomes to remove protein aggregates and damaged organelles from the cytoplasm for recycling. Hepatitis C virus (HCV) has been shown to induce autophagy to enhance its own replication. Here we describe a procedure that combines membrane flotation and affinity chromatography for the purification of autophagosomes from cells that harbor a HCV subgenomic RNA replicon. The purified autophagosomes had double- or multiple- membrane structures with a diameter ranging from 200 nm to 600 nm...
July 26, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28741517/filling-the-gap-on-caveolin-1-in-liver-carcinogenesis
#16
Manuel A Fernandez-Rojo, Grant A Ramm
Caveolin-1 (CAV1) has emerged as a promoter of proliferation, metastasis, and chemoresistance in hepatoma cells, as well as a marker of poor prognosis in liver cancer. We discuss here current knowledge and future approaches to elucidating the molecular mechanisms underlying CAV1 action during hepatocarcinogenesis and evaluate its potential use in clinical therapies.
December 2016: Trends in Cancer
https://www.readbyqxmd.com/read/28735300/caveolin-1-expression-as-a-prognostic-marker-in-triple-negative-breast-cancers-of-asian-women
#17
Joe Yeong, Aye Aye Thike, Murasaki Ikeda, Jeffrey Chun Tatt Lim, Bernett Lee, Seigo Nakamura, Jabed Iqbal, Puay Hoon Tan
BACKGROUND: Triple-negative breast cancers (TNBCs) are defined by their lack of oestrogen receptor, progesterone receptor and epidermal growth factor receptor 2. Although heterogeneous, the majority are aggressive and treatment options are limited. Caveolin acts as tumour suppressor or promoter depending on the cancer type. AIM: In this study, we aimed to determine if the expression levels of the candidate biomarker caveolin-1 on stromal or tumour cells were associated with clinicopathological parameters and disease outcomes in TNBCs from an ethnically diverse cohort of Asian women...
July 22, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28734243/caveolin-1-related-autophagy-initiated-by-aldosterone-induced-oxidation-promotes-liver-sinusoidal-endothelial-cells-defenestration
#18
Xiaoying Luo, Dan Wang, Xuan Luo, Xintao Zhu, Guozhen Wang, Zuowei Ning, Yang Li, Xiaoxin Ma, Renqiang Yang, Siyi Jin, Yun Huang, Ying Meng, Xu Li
Aldosterone, with pro-oxidation and pro-autophagy capabilities, plays a key role in liver fibrosis. However, the mechanisms underlying aldosterone-promoted liver sinusoidal endothelial cells (LSECs) defenestration remain unknown. Caveolin 1 (Cav1) displays close links with autophagy and fenestration. Hence, we aim to investigate the role of Cav1-related autophagy in LSECs defenestration. We found the increase of aldosterone/MR (mineralocorticoid receptor) level, oxidation, autophagy, and defenestration in LSECs in the human fibrotic liver, BDL or hyperaldosteronism models; while antagonizing aldosterone or inhibiting autophagy relieved LSECs defenestration in BDL-induced fibrosis or hyperaldosteronism models...
July 13, 2017: Redox Biology
https://www.readbyqxmd.com/read/28729092/annexin-a2-modulated-proliferation-of-pulmonary-arterial-smooth-muscle-cells-depends-on-caveolae-and-caveolin-1-in-hepatopulmonary-syndrome
#19
Lin Liao, Binwu Zheng, Bin Yi, Chang Liu, Lin Chen, Ziyang Zeng, Jing Gao
We have established that annexin A2 (ANXA2) is an important factor in the experimental hepatopulmonary syndrome (HPS) serum-induced proliferation of pulmonary arterial smooth muscle cells (PASMCs). However, the detailed mechanism remains unclear. ANXA2 translocated to the caveolin-enriched microdomains (caveolae) in PASMCs upon HPS serum stimulation. The disruption of caveolae by Methyl-β-cyclodextrin (MβCD) alleviated the caveolae recruitment of ANXA2 and the ANXA2-mediated activation of ERK1/2 and NF-κB, so that ANXA2-modulated PASMC proliferation was suppressed...
July 17, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28723669/multicomponent-analysis-of-the-tumour-microenvironment-reveals-low-cd8-t-cell-number-low-stromal-caveolin-1-and-high-tenascin-c-and-their-combination-as-significant-prognostic-markers-in-non-small-cell-lung-cancer
#20
David Onion, Mark Isherwood, Naveen Shridhar, Mikalena Xenophontos, Madeleine L Craze, Laura J Day, María A García-Márquez, Robert G Pineda, Alexander M Reece-Smith, John H Saunders, John P Duffy, Richard H Argent
The complex interplay of the tumour microenvironment (TME) and its role in disease progression and response to therapy is poorly understood. The majority of studies to date focus on individual components or molecules within the TME and so lack the power correlative analysis. Here we have performed a multi-parameter analysis of the TME in 62 resectable non-small cell lung cancer (NSCLC) specimens detailing number and location of immune infiltrate, assessing markers of cancer-associated fibroblasts, caveolin-1 and tenascin-C, and correlating with clinicopathological details, as well as markers of disease progression such as epithelial-to-mesenchymal transition (EMT)...
June 29, 2017: Oncotarget
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