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https://www.readbyqxmd.com/read/28318118/distinct-expression-of-nk2-homeobox-1-nkx2-1-and-goblet-cell-hyperplasia-in-nasal-polyps-with-different-endotypes
#1
Jintao Du, Luo Ba, Bo Li, Feng Liu, Xianting Hu, Jie Zhang, Yafeng Liu, Junming Xian, Sixi Liu, Huabin Li
BACKGROUND: Decreased expression of airway epithelial-specific transcription factor NK2 homeobox 1 (NKX2-1) was associated with allergic inflammation in asthma patients. However, the expression and role of NKX2-1 in nasal polyps (NPs) with different endotypes were undefined yet. METHODS: We examined the expression of key cytokines (interleukin [IL]-4 IL-5, IL-13, and IL-17A, etc.) and NKX2-1 in NPs with different endotypes and control tissues by immunohistochemistry staining, qualitative polymerase chain reaction (qPCR), enzyme-linked immunosorbent assay (ELISA), and Western blot analysis...
March 20, 2017: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/28315439/interaction-of-chitin-chitosan-with-salivary-and-other-epithelial-cells-an-overview
#2
REVIEW
Sharvari Vijaykumar Patil, Lalitha S Y Nanduri
Chitin and its deacetylated form, chitosan, have been widely used for tissue engineering of both epithelial and mesenchymal tissues. Epithelial cells characterised by their sheet-like tight cellular arrangement and polarised nature, constitute a major component in various organs and play a variety of roles including protection, secretion and maintenance of tissue homeostasis. Regeneration of damaged epithelial tissues has been studied using biomaterials such as chitin, chitosan, hyaluronan, gelatin and alginate...
March 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28291371/activity-of-multidrug-resistance-associated-proteins-1-5-mrp1-5-in-the-rpmi-2650-cell-line-and-explants-of-human-nasal-turbinate
#3
Anne M Dolberg, Stephan Reichl
The profound influence of ATP-binding cassette (ABC) transporters on the disposition of numerous drugs has led to increased interest in characterizing their expression profiles in various epithelial and endothelial barriers. The present work examined the presence and functional activity of five ABC efflux proteins, i.e. MRP 1 - 5, in freshly isolated human nasal epithelial cells and two in vitro models based on the human RPMI 2650 cell line. To evaluate the expression patterns of MRP1, MRP2, MRP3, MRP4 and MRP5 at the mRNA and protein levels in the ex vivo model and the differently cultured RPMI 2650 cells, reverse transcriptase polymerase chain reaction (RT-PCR), Western blot analysis and indirect immunofluorescence staining were used...
March 14, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28289476/dna-methylation-at-modifier-genes-of-lung-disease-severity-is-altered-in-cystic-fibrosis
#4
Milena Magalhães, Isabelle Rivals, Mireille Claustres, Jessica Varilh, Mélodie Thomasset, Anne Bergougnoux, Laurent Mely, Sylvie Leroy, Harriet Corvol, Loïc Guillot, Marlène Murris, Emmanuelle Beyne, Davide Caimmi, Isabelle Vachier, Raphaël Chiron, Albertina De Sario
BACKGROUND: Lung disease progression is variable among cystic fibrosis (CF) patients and depends on DNA mutations in the CFTR gene, polymorphic variations in disease modifier genes, and environmental exposure. The contribution of genetic factors has been extensively investigated, whereas the mechanism whereby environmental factors modulate the lung disease is unknown. In this project, we hypothesized that (i) reiterative stress alters the epigenome in CF-affected tissues and (ii) DNA methylation variations at disease modifier genes modulate the lung function in CF patients...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28278175/pneumococcal-infection-of-respiratory-cells-exposed-to-welding-fumes-role-of-oxidative-stress-and-hif-1-alpha
#5
Jonathan Grigg, Lisa Miyashita, Reetika Suri
Welders are more susceptible to pneumococcal pneumonia. The mechanisms are yet unclear. Pneumococci co-opt the platelet activating factor receptor (PAFR) to infect respiratory epithelial cells. We previously reported that exposure of respiratory cells to welding fumes (WF), upregulates PAFR-dependent pneumococcal infection. The signaling pathway for this response is unknown, however, in intestinal cells, hypoxia-inducible factor-1 α (HIF 1α) is reported to mediate PAFR-dependent infection. We sought to assess whether oxidative stress plays a role in susceptibility to pneumococcal infection via the platelet activating factor receptor...
2017: PloS One
https://www.readbyqxmd.com/read/28259510/comparison-of-inflammatory-cytokine-release-from-nasal-epithelial-cells-of-non-atopic-non-rhinitic-allergic-rhinitic-and-polyp-subjects-and-effects-of-diesel-exhaust-particles-in-vitro
#6
A B Ozturk, R Bayraktar, B Gogebakan, S Mumbuc, H Bayram
BACKGROUND: Although studies have reported an association between air pollutants and increased allergic airway diseases, such as allergic rhinitis and nasal polyposis, the underlying mechanisms are not fully understood. A limited number of studies have suggested that diesel exhaust particles (DEP) play a role in atopy and the pathogenesis of allergic upper airway diseases. The aim of this study was to investigate the effect of DEP on inflammatory cytokine release, and mRNA expression of transcription factors such as JNK and NF-β in primary nasal epithelial cells (NECs), in vitro...
March 2, 2017: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/28258963/interferon-%C3%AE-induced-insufficient-autophagy-contributes-to-p62-dependent-apoptosis-of-epithelial-cells-in-chronic-rhinosinusitis-with-nasal-polyps
#7
Bao-Feng Wang, Ping-Ping Cao, Zhi-Chao Wang, Zhi-Yong Li, Zhe-Zheng Wang, Jin Ma, Bo Liao, Yi-Ke Deng, Xiao-Bo Long, Kai Xu, Heng Wang, Hai Wang, Ming Zeng, Xiang Lu, Zheng Liu
BACKGROUND: Autophagy is a lysosomal degradation pathway that is essential for cell survival, differentiation, and homeostasis. This study aimed to investigate the contribution of autophagy to the pathogenesis of CRS with nasal polyps (CRSwNP). METHODS: The expression of autophagic proteins [microtubule-associated protein 1 light chain 3B (LC3B)-II, autophagy-related proteins (Atg), and Beclin 1], substrate proteins (p62 and ubiquitinated proteins), and apoptotic signaling molecules [cysteine-aspartic protease-3 and 8, and poly-ADP-ribose polymerase] in the sinonasal mucosa and nasal epithelial cells (NECs) was detected by immunohistochemistry and Western blotting...
March 4, 2017: Allergy
https://www.readbyqxmd.com/read/28251016/assessment-of-pasteurella-multocida-a-lipopolysaccharide-as-an-adhesin-in-an-in-vitro-model-of-rabbit-respiratory-epithelium
#8
Carolina Gallego, Stefany Romero, Paula Esquinas, Pilar Patiño, Nhora Martínez, Carlos Iregui
The role of the P. multocida lipopolysaccharide (LPS) as a putative adhesin during the early stages of infection with this bacterium in the respiratory epithelium of rabbits was investigated. By light microscopy and double enzyme labeling of nasal septa tissues, the amount of bacteria attached to the respiratory epithelium and the amount of LPS present in goblet cells at different experimental times were estimated. Transmission electron microscopy (TEM) and LPS labeling with colloidal gold particles were also used to determine the exact location of LPS in the cells...
2017: Veterinary Medicine International
https://www.readbyqxmd.com/read/28245149/airborne-particulate-matter-induces-non-allergic-eosinophilic-sinonasal-inflammation-in-mice
#9
Murugappan Ramanathan, Nyall R London, Anuj Tharakan, Nitya Surya, Thomas E Sussan, Xiaoquan Rao, Sandra Y Lin, Elina Toskala, Sanjay Rajagopalan, Shyam Biswal
RATIONALE: Exposure to airborne particulate matter has been linked to aggravation of respiratory symptoms, increased risk of cardiovascular disease, and all-cause mortality. While the health effects of particulate matter on the lower pulmonary airway have been extensively studied, little is known regarding the impact of chronic particulate matter exposure on the upper sinonasal airway. OBJECTIVES: To test the impact of chronic airborne particulate matter exposure on the upper respiratory system in vivo...
February 28, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28241864/ccl2-and-ccl5-driven-attraction-of-cd172a-monocytic-cells-during-an-equine-herpesvirus-type-1-ehv-1-infection-in-equine-nasal-mucosa-and-the-impact-of-two-migration-inhibitors-rosiglitazone-rsg-and-quinacrine-qc
#10
Jing Zhao, Katrien C K Poelaert, Jolien Van Cleemput, Hans J Nauwynck
Equine herpesvirus type 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. Besides epithelial cells, CD172a(+) monocytic cells become infected with EHV-1 in the respiratory mucosa and transport the virus from the apical side of the epithelium to the lamina propria en route to the lymph and blood circulation. Whether CD172a(+) monocytic cells are specifically recruited to the infection sites in order to pick up virus is unknown. In our study, equine nasal mucosa explants were inoculated with EHV-1 neurological strains 03P37 and 95P105 or the non-neurological strains 97P70 and 94P247 and the migration of monocytic cells was examined by immunofluorescence...
February 27, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28238741/microparticles-in-nasal-lavage-fluids-in-chronic-rhinosinusitis-potential-biomarkers-for-diagnosis-of-aspirin-exacerbated-respiratory-disease
#11
Toru Takahashi, Atsushi Kato, Sergejs Berdnikovs, Whitney W Stevens, Lydia A Suh, James Norton, Roderick G Carter, Kathleen E Harris, Anju T Peters, Kathryn E Hulse, Leslie C Grammer, Kevin Welch, Stephanie Shintani-Smith, Bruce K Tan, David B Conley, Robert C Kern, Bruce S Bochner, Robert P Schleimer
BACKGROUND: Microparticles (MPs) are submicron sized shed membrane vesicles released from activated or injured cells and are detectable by flow cytometry. MP levels have been utilized as biomarkers to evaluate cell injury or activation in patients with pathological conditions. OBJECTIVE: To compare MP types and levels in nasal lavage fluids (NLFs) from controls and patients with chronic rhinosinusitis without nasal polyps (CRSsNP), CRS with NP (CRSwNP) and aspirin exacerbated respiratory disease (AERD)...
February 23, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28235419/a-rabbit-model-for-experimental-alveolar-cleft-grafting
#12
Mohammad Kamal, Lars Andersson, Rene Tolba, Alexander Bartella, Felix Gremse, Frank Hölzle, Peter Kessler, Bernd Lethaus
OBJECTIVES: The purpose of the present study was to develop an animal model for creating alveolar cleft defects with properly simulated clinical defect environment for tissue-engineered bone-substitute materials testing without compromising the health of the animal. Cleft creation surgery was aimed at creating a complete alveolar cleft with a wide bone defect with an epithelial lining (oral mucosa) overlying the cleft defect. METHODS: A postmortem skull of a New Zealand White (NZW) rabbit skull (Oryctolagus cuniculus) underwent an osteological and imaging survey...
February 24, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28219705/substance-p-represents-a-novel-first-line-defense-mechanism-in-the-nose
#13
Olivia Larsson, Lotta Tengroth, Yuan Xu, Rolf Uddman, Susanna Kumlien Georén, Lars-Olaf Cardell
BACKGROUND: Neuropeptides, such as substance P (SP), have long been seen as mediators of widespread, continuous airway inflammation, a process known as neurogenic inflammation. However, this has been difficult to demonstrate clinically, suggesting an alternative role for these signaling molecules. OBJECTIVES: To examine the role of SP in nasal infection, by assessing the release of SP in response to viral stimulation and characterizing the effects of SP on innate immunity, the latter reflected in changes in local Toll-like receptor (TLR) expression...
February 17, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28216437/extracellular-eosinophilic-traps-in-association-with-staphylococcus-aureus-at-the-site-of-epithelial-barrier-defects-in-patients-with-severe-airway-inflammation
#14
Elien Gevaert, Nan Zhang, Olga Krysko, Feng Lan, Gabriële Holtappels, Natalie De Ruyck, Hans Nauwynck, Shida Yousefi, Hans-Uwe Simon, Claus Bachert
BACKGROUND: Chronic rhinosinusitis with nasal polyps is characterized by TH2-biased eosinophilic inflammation. Eosinophils have been shown to generate so-called extracellular eosinophilic traps (EETs) under similar pathologic conditions. OBJECTIVE: Our aim was to investigate a possible link between EET formation and the presence of Staphylococcus aureus, an organism frequently colonizing the upper airways, at the human mucosal site of the disease. METHODS: Tissue slides were investigated for the presence of EETs and S aureus by using immunofluorescent staining and the PNA-Fish assay, respectively...
February 17, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28207789/carbapenem-resistance-and-pathogenicity-of-bovine-acinetobacter-indicus-like-isolates
#15
Peter Klotz, Stephan Göttig, Ursula Leidner, Torsten Semmler, Sandra Scheufen, Christa Ewers
The objective of this study was to characterize blaOXA-23 harbouring Acinetobacter indicus-like strains from cattle including genomic and phylogenetic analyses, antimicrobial susceptibility testing and evaluation of pathogenicity in vitro and in vivo. Nasal and rectal swabs (n = 45) from cattle in Germany were screened for carbapenem-non-susceptible Acinetobacter spp. Thereby, two carbapenem resistant Acinetobacter spp. from the nasal cavities of two calves could be isolated. MALDI-TOF mass spectrometry and 16S rDNA sequencing identified these isolates as A...
2017: PloS One
https://www.readbyqxmd.com/read/28195747/do-aging-factors-influence-the-clinical-presentation-and-management-of-chronic-rhinosinusitis
#16
Axel E Renteria, Leandra Mfuna Endam, Martin Desrosiers
Objective/Hypothesis Chronic rhinosinusitis (CRS) is a complex inflammatory disease of the upper respiratory airways resulting from the dysregulation of immunity and epithelial defenses. More recently, the contribution of an altered nasal microbiome to the development of CRS has also been proposed. However, the impact of aging on the development of CRS has been long overlooked. Here we propose, in a hypothesis piece, that aging can influence the physiopathology of CRS and its subsequent management in an elderly population...
February 1, 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/28193223/malondialdehyde-acetaldehyde-maa-adducted-surfactant-protein-induced-lung-inflammation-is-mediated-through-scavenger-receptor-a-sr-a1
#17
Muna Sapkota, Jane M DeVasure, Kusum K Kharbanda, Todd A Wyatt
BACKGROUND: Co-exposure to cigarette smoke and alcohol leads to the generation of high concentrations of acetaldehyde and malondialdehyde in the lung. These aldehydes being highly electrophilic in nature react with biologically relevant proteins such as surfactant protein D (SPD) through a Schiff base reaction to generate SPD adducted malondialdehyde-acetaldehyde adduct (SPD-MAA) in mouse lung. SPD-MAA results in an increase in lung pro-inflammatory chemokine, keratinocyte chemoattractant (KC), and the recruitment of lung lavage neutrophils...
February 13, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28191699/ndrg1-is-important-to-maintain-the-integrity-of-airway-epithelial-barrier-through-claudin-9-expression
#18
Yasuhiro Gon, Shuichiro Maruoka, Hiroyuki Kishi, Yutaka Kozu, Kuroda Kazumichi, Yasuyuki Nomura, Ikuko Takeshita, Takeshi Oshima, Shu Hashimoto
Impairment of epithelial barrier integrity caused by environmental triggers is associated with the pathogenesis of airway inflammation. Using human airway epithelial cells, we attempted to identify molecule(s) that promote airway epithelial barrier integrity. Microarray analyses were conducted using the Affimetrix human whole genome gene chip, and we identified the N-myc downstream-regulated gene 1 (NDRG1) gene, which was induced during the development of the epithelial cell barrier. Immunohistochemical analysis revealed strong NDRG1 expression in ciliated epithelial cells in nasal tissues sampled from patients with chronic rhinosinusitis (CRS), and the low expression of NDRG1 was observed in goblet cells or damaged epithelial cells...
February 13, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28183201/effect-of-titanium-dioxide-nanoparticles-tio2-nps-on-the-expression-of-mucin-genes-in-human-airway-epithelial-cells
#19
Gui Ok Kim, Yoon Seok Choi, Chang Hoon Bae, Si-Youn Song, Yong-Dae Kim
OBJECTIVE: Titanium dioxide nanoparticles (TiO2 NPs) are utilized with growing frequency for a wide variety of industrial applications. Recently, acute and chronic exposures to TiO2 NPs have been found to induce inflammatory response in the human respiratory tract. However, the effect and mechanism underlying the induction of major airway mucins by TiO2 NPs have not been elucidated. This study was conducted to characterize the effect of TiO2 NPs, and the mechanism involved, on the expressions of airway mucins in human airway epithelial cells...
January 2017: Inhalation Toxicology
https://www.readbyqxmd.com/read/28178421/lactoferrin-interacts-with-splunc1-to-attenuate-lipopolysaccharide-induced-inflammation-of-human-nasal-epithelial-cells-via-down-regulated-mek1-2-mapk-signaling
#20
Yung-An Tsou, Yu-Tong Tung, Tsu-Fang Wu, Gary Ro-Lin Chang, Han-Chien Chen, Chia-Der Lin, Chih-Ho Lai, Hsiao-Ling Chen, Chuan-Mu Chen
The short palate, lung, and nasal epithelium clone 1 (SPLUNC1) protein is an important innate material in the upper airway, and lactoferrin (LF) aids the innate functions in humans. In this study, an nasal epithelial model was used to investigate how LF modulates SPLUNC1 to reduce the inflammatory process mediated by lipopolysaccharide (LPS). The inflammation of human RPMI-2650 cells was induced by LPS to evaluate SPLUNC1 expression after treating the cells with bovine LF (bLF). The interaction pathway between LF and SPLUNC1 in LPS-induced inflammation was further investigated...
February 8, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
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