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https://www.readbyqxmd.com/read/28811631/development-of-a-primary-human-co-culture-model-of-inflamed-airway-mucosa
#1
Lael M Yonker, Hongmei Mou, Kengyeh K Chu, Michael A Pazos, Huimin Leung, Dongyao Cui, Jinhyeob Ryu, Rhianna M Hibbler, Alexander D Eaton, Tim N Ford, J R Falck, T Bernard Kinane, Guillermo J Tearney, Jayaraj Rajagopal, Bryan P Hurley
Neutrophil breach of the mucosal surface is a common pathological consequence of infection. We present an advanced co-culture model to explore neutrophil transepithelial migration utilizing airway mucosal barriers differentiated from primary human airway basal cells and examined by advanced imaging. Human airway basal cells were differentiated and cultured at air-liquid interface (ALI) on the underside of 3 µm pore-sized transwells, compatible with the study of transmigrating neutrophils. Inverted ALIs exhibit beating cilia and mucus production, consistent with conventional ALIs, as visualized by micro-optical coherence tomography (µOCT)...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28805732/the-mechanistic-links-between-insulin-and-cystic-fibrosis-transmembrane-conductance-regulator-cftr-cl-channel
#2
REVIEW
Yoshinori Marunaka
The cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel belongs to the ATP-binding cassette (ABC) transporter superfamily and regulates Cl(-) secretion in epithelial cells for water secretion. Loss-of-function mutations to the CFTR gene cause dehydrated mucus on the apical side of epithelial cells and increase the susceptibility of bacterial infection, especially in the airway and pulmonary tissues. Therefore, research on the molecular properties of CFTR, such as its gating mechanism and subcellular trafficking, have been intensively pursued...
August 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28801844/brg1-inhibits-e-cadherin-expression-in-lung-epithelial-cells-and-disrupts-epithelial-integrity
#3
Ting Wang, Wenjing Zou, Chao Niu, Fengxia Ding, Yaping Wang, Shuang Cai, Hua Zhu, Daiyin Tian, Jihong Dai, Enmei Liu, Qing Lu, Zhou Fu, Lin Zou
Brahma-related gene-1 (Brg1), a key chromatin remodeling factor, is associated with cell proliferation and migration in kidney and heart cells, but few reports have examined its role in airway epithelial cell. Airway epithelial injury, which is involved in the entire pathological process of asthma, is an important cause of recurrent asthma. Here, we studied the function of Brg1 in an ovalbumin (OVA)-induced asthma model (lung-specific conditional Brg1 (Brg1(-/-)) knockdown mice) and human bronchial epithelial 16HBE cells stably expressing Brg1 shRNA...
August 11, 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/28798252/pollen-induced-oxidative-dna-damage-response-regulates-mirnas-controlling-allergic-inflammation
#4
Leopoldo Aguilera-Aguirre, Wenjing Hao, Lang Pan, Xiaoxue Li, Alfredo Saavedra-Molina, Attila Bacsi, Zsolt Radak, Sanjiv Sur, Allan R Brasier, Xueqing Ba, Istvan Boldogh
A mucosal oxidative burst is a hallmark response to pollen exposure that promotes allergic inflammatory responses. Reactive species constituents of oxidative stress signal via the modification of cellular molecules including nucleic acids. One of the most abundant oxidative genomic base damage is 8-oxo-7,8-dihydroguanine (8-oxoG), which is removed from DNA by 8-oxoguanine DNA glycosylase1 (OGG1). OGG1 in complex with 8-oxoG acts as a GDP-GTP exchange factor and induces acute inflammation; however, the mechanism(s) by which OGG1 signaling regulates allergic airway inflammation is not known...
August 10, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28795606/hydrogen-rich-saline-inhibits-tobacco-smoke-induced-chronic-obstructive-pulmonary-disease-by-alleviating-airway-inflammation-and-mucus-hypersecretion-in-rats
#5
Zibing Liu, Wenye Geng, Chuanwei Jiang, Shujun Zhao, Yong Liu, Ying Zhang, Shucun Qin, Chenxu Li, Xinfang Zhang, Yanhong Si
Chronic obstructive pulmonary disease induced by tobacco smoke has been regarded as a great health problem worldwide. The purpose of this study is to evaluate the protective effect of hydrogen-rich saline, a novel antioxidant, on chronic obstructive pulmonary disease and explore the underlying mechanism. Sprague-Dawley rats were made chronic obstructive pulmonary disease models via tobacco smoke exposure for 12 weeks and the rats were treated with 10 ml/kg hydrogen-rich saline intraperitoneally during the last 4 weeks...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28794044/nidovirus-associated-proliferative-pneumonia-in-the-green-tree-python-morelia-viridis
#6
Eva Dervas, Jussi Hepojoki, Andrea Laimbacher, Fernando Romero-Palomo, Christine Jelinek, Saskia Keller, Teemu Smura, Satu Hepojoki, Anja Kipar, Udo Hetzel
In 2014 we observed a noticeable increase in sudden deaths of green tree pythons (Morelia viridis). Pathological examination revealed accumulation of mucoid material within airways and lung, associated with enlargement of the entire lung. We performed full necropsy and histological examination on 12 affected green tree pythons from 7 different breeders to characterise the pathogenesis of this "mucinous" pneumonia. By histology we could show a marked hyperplasia of the airway epithelium and of faveolar type II pneumocytes...
August 9, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28790149/evaluation-of-endotracheal-tube-scraping-on-airway-resistance
#7
J Brady Scott, Meagan N Dubosky, David L Vines, Adewunmi S Sulaiman, Kyle R Jendral, Gagan Singh, Ankeet Patel, Carl A Kaplan, David P Gurka, Robert A Balk
BACKGROUND: Spontaneous breathing trials (SBTs) are used to assess the readiness for discontinuation of mechanical ventilation. When airway resistance (Raw) is elevated, the imposed work of breathing can lead to prolongation of mechanical ventilation. Biofilm and mucus build-up within the endotracheal tube (ETT) can increase Raw. Scraping the ETT can remove the biofilm build-up and decrease mechanical Raw. The primary aim of this study was to evaluate the impact of ETT scraping on Raw...
August 8, 2017: Respiratory Care
https://www.readbyqxmd.com/read/28780505/local-dornase-alfa-treatment-reduces-nets-induced-airway-obstruction-during-severe-rsv-infection
#8
Bart Cortjens, Rineke de Jong, Judith G Bonsing, Job B M van Woensel, Adriaan F G Antonis, Reinout A Bem
Respiratory syncytial virus (RSV) infection is characterised by airway obstruction with mucus plugs, containing DNA networks in the form of neutrophil extracellular traps (NETs). We investigated the effect of dornase alfa on histopathological NETs-induced airway obstruction and viral load in an age-relevant calf model of severe bovine RSV disease. As compared with the control animals, dornase alfa treatment resulted in a strong reduction of NETs-induced airway obstruction. Viral load in the lower respiratory tract was not different between the two groups...
August 5, 2017: Thorax
https://www.readbyqxmd.com/read/28776693/a-comparison-of-tracheal-scaffold-strategies-for-pediatric-transplantation-in-a-rabbit-model
#9
Elizabeth F Maughan, Colin R Butler, Claire Crowley, Gui Zhen Teoh, Margot Den Hondt, Nicholas J Hamilton, Robert E Hynds, Peggy Lange, Tahera Ansari, Luca Urbani, Samuel M Janes, Paolo De Coppi, Martin A Birchall, Martin J Elliott
OBJECTIVES/HYPOTHESIS: Despite surgical advances, childhood tracheal stenosis is associated with high morbidity and mortality. Various tracheal scaffold strategies have been developed as the basis for bioengineered substitutes, but there is no consensus on which may be superior in vivo. We hypothesized that there would be no difference in morbidity and mortality between three competing scaffold strategies in rabbits. STUDY DESIGN: Pilot preclinical study. METHODS: Tracheal scaffolds were prepared by three methods that have been applied clinically and reported: preserved cadaveric ("Herberhold") allografts, detergent-enzymatically decellularized allografts, and synthetic scaffolds (nanocomposite polymer [polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (POSS-PCU)])...
August 4, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28775098/influenza-virus-infection-alters-ion-channel-function-of-airway-and-alveolar-cells-mechanisms-and-physiological-sequelae
#10
James D Londino, Ahmed Lazrak, James F Collawn, Zsuzsanna Bebok, Kevin S Harrod, Sadis Matalon
The cystic fibrosis transmembrane conductance regulator (CFTR) and the amiloride-sensitive epithelial sodium channels (ENaC) are located in the apical membranes of airway and alveolar epithelial cells. These transporters play an important role in the regulation of lung fluid balance across airway and alveolar epithelia by being the conduits for chloride (Cl(-)) and bicarbonate (HCO3(-)) secretion and sodium (Na(+)) ion absorption, respectively. The functional role of these channels in the respiratory tract is to maintain the optimum volume and ionic composition of the bronchial pericilary fluid (PCL) and alveolar lining fluid (ALF) layers...
August 3, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28765819/a-review-of-42-asthmatic-children-with-allergic-bronchopulmonary-aspergillosis
#11
Ashok Shah, Shekhar Kunal
BACKGROUND: Allergic bronchopulmonary aspergillosis (ABPA) in children with asthma, not associated with cystic fibrosis, is yet to receive the recognition it deserves. OBJECTIVE: To highlight the presentation of ABPA in children with asthma. METHODS: This retrospective review documents the occurrence of pediatric ABPA over a period of 31 years in one unit. Children with asthma, eosinophilia and infiltrates on chest radiograph were screened for ABPA...
July 2017: Asia Pacific Allergy
https://www.readbyqxmd.com/read/28758330/thermo-physical-properties-of-synthetic-mucus-for-the-study-of-airway-clearance
#12
O Lafforgue, N Bouguerra, S Poncet, I Seyssiecq, J Favier, S Elkoun
In this paper, dynamic viscosity, surface tension, density, heat capacity and thermal conductivity, of a bronchial mucus simulant proposed by Zahm et al. [1] were experiementally determined. This simulant is mainly composed of a galactomannan gum and a scleroglucan. It was shown that thermophysical properties of synthetic mucus are dependant of scleroglucan concentrations. More importantly and for some scleroglucan concentrations, the syntetic mucus, exhibits, somehow, comparable thermophysical propoerties to real bronchial mucus...
July 30, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28756367/the-participation-of-oxidative-stress-in-the-pathogenesis-of-bronchial-asthma
#13
REVIEW
Paulina Kleniewska, Rafał Pawliczak
Reactive oxygen species are produced during oxygen reduction and are characterized by high reactivity. They participate in many important physiological processes, but if produced in high concentrations they lead to oxidative stress development and disturb pro-oxidative/anti-oxidative balance towards the oxidation reaction - leading to damage of lipids, proteins, carbohydrates or nucleic acids. Asthma is a chronic inflammatory disease of the airways of various pathogenesis and clinical symptoms, prevalence in recent years has increased significantly...
July 27, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28746467/tangeretin-has-anti-asthmatic-effects-via-regulating-pi3k-and-notch-signaling-and-modulating-th1-th2-th17-cytokine-balance-in-neonatal-asthmatic-mice
#14
L-L Liu, F-H Li, Y Zhang, X-F Zhang, J Yang
Asthma is a chronic allergic disease characterized by airway inflammation, airway hyper-responsiveness (AHR), and mucus hypersecretion. T-lymphocytes are involved in the pathogenesis of asthma, mediating airway inflammatory reactions by secreting cytokines. The phosphoinositide 3-kinase (PI3K) and Notch signaling pathways are associated with T cell signaling, proliferation, and differentiation, and are important in the progression of asthma. Thus, compounds that can modulate T cell proliferation and function may be of clinical value...
July 20, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28746416/temporal-dynamics-of-ovine-airway-epithelial-cell-differentiation-at-an-air-liquid-interface
#15
Nicky O'Boyle, Erin Sutherland, Catherine C Berry, Robert L Davies
The respiratory tract and lungs are subject to diverse pathologies with wide-ranging implications for both human and animal welfare. The development and detailed characterization of cell culture models for studying such forms of disease is of critical importance. In recent years the use of air-liquid interface (ALI)-cultured airway epithelial cells has increased markedly, as this method of culture results in the formation of a highly representative, organotypic in vitro model system. In this study we have expanded on previous knowledge of differentiated ovine tracheal epithelial cells by analysing the progression of differentiation over an extensive time course at an ALI...
2017: PloS One
https://www.readbyqxmd.com/read/28735728/artemisia-argyi-attenuates-airway-inflammation-in-ovalbumin-induced-asthmatic-animals
#16
Na-Rae Shin, Hyung-Won Ryu, Je-Won Ko, Sung-Hyeuk Park, Heung-Joo Yuk, Ha-Jung Kim, Jong-Choon Kim, Seong-Hun Jeong, In-Sik Shin
ETHNOPHARMACOLOGICAL RELEVANCE: Artemisia argyi is a traditional herbal medicine in Korea and commonly called as mugwort. It is traditionally used as food source and tea to control abdominal pain, dysmenorrhea, uterine hemorrhage, and inflammation. AIM OF THE STUDY: We investigated the effects of A. argyi (TOTAL) and dehydromatricarin A (DA), its active component on ovalbumin (OVA)-induced allergic asthma. MATERIALS AND METHODS: The animals were sensitized on day 0 and 14 by intraperitoneal injection of OVA with aluminum hydroxide...
July 20, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28733314/effects-of-an-airway-clearance-device-on-inflammation-bacteriology-and-mucus-transport-in-bronchiectasis
#17
Joana Tambascio, Hugo Celso Dutra de Souza, Roberto Martinez, José Antônio Baddini-Martinez, Peter John Barnes, Ada Clarice Gastaldi
BACKGROUND: Bronchiectasis is characterized by abnormal and permanent dilation of the bronchi, caused by the perpetuation of inflammation and impairment of mucociliary clearance. Physiotherapy techniques can help to decrease the retention of respiratory secretions. The flutter valve combines high-frequency oscillation and positive expiratory pressure to facilitate the removal of secretions. We evaluated the effects of the flutter valve on sputum inflammation, microbiology, and transport of respiratory secretions in patients with bronchiectasis...
August 2017: Respiratory Care
https://www.readbyqxmd.com/read/28732801/bicarbonate-in-cystic-fibrosis
#18
REVIEW
Karl Kunzelmann, Rainer Schreiber, Hans Beat Hadorn
BACKGROUND: Cystic fibrosis (CF, mucoviscidosis) is caused by mutations in the gene encoding CF transmembrane conductance regulator (CFTR), which is a chloride and bicarbonate channel necessary for fluid secretion and extracellular alkalization. For a long time, research concentrated on abnormal Cl(-) and Na(+) transport, but neglected bicarbonate as a crucial factor in CF. METHODS: The present short review reports early findings as well as recent insights into the role of CFTR for bicarbonate transport and its defects in CF...
July 18, 2017: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/28731127/pidotimod-exacerbates-allergic-pulmonary-infection-in-an-ova-mouse-model-of-asthma
#19
Luo-Qin Fu, Ya-Li Li, Ai-Kun Fu, Yan-Ping Wu, Yuan-Yuan Wang, Sheng-Lan Hu, Wei-Fen Li
Pidotimod is a synthetic dipeptide with biological and immuno‑modulatory properties. It has been widely used for treatment and prevention of recurrent respiratory infections. However, its impact on the regulation of allergic pulmonary inflammation is still not clear. In the current study, an ovalbumin (OVA)‑induced allergic asthma model was used to investigate the immune‑modulating effects of pidotimod on airway eosinophilia, mucus metaplasia and inflammatory factor expression compared with dexamethasone (positive control)...
July 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28730380/acute-and-chronic-effects-of-oral-erdosteine-on-ciliary-beat-frequency-cough-sensitivity-and-airway-reactivity
#20
L Pappová, I Kazimierová, M Jošková, M Šutovská, S Fraňová
Erdosteine as a mucolytic agent that decreases mucus viscosity and facilitates mucus expulsion from the airways by cough or ciliary movement. Our objective was to determine whether erdosteine can directly contribute to mucus clearance. We addressed the issue by monitoring acute and chronic effects of erdosteine on ciliary beat frequency (CBF), cough sensitivity, and airway smooth muscle reactivity. The experiments were performed in healthy guinea pigs. Erdosteine (10 mg/kg) was administrated orally in a single dose or daily through 7 days...
July 21, 2017: Advances in Experimental Medicine and Biology
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