keyword
MENU ▼
Read by QxMD icon Read
search

Defect

keyword
https://www.readbyqxmd.com/read/29149699/tetrahymena-cilia-cap-is-built-in-a-multi-step-process-a-study-by-atomic-force-microscopy
#1
Cecília Seixas, João Gonçalves, Luís Viseu Melo, Helena Soares
Cilia are complex and dynamic organelles that have motility and sensory functions. Defects in cilia biogenesis and function are at the origin of human ciliopathies. In motile cilia, a basal body organizes the axoneme composed of nine microtubule doublets surrounding a central pair of singlet microtubules. The distal ends of axonemal microtubules are attached to the membrane by microtubule-capping structures. Little is known about the early steps of cilium assembly. Although cilia grow and resorb from their distal tips, it remains poorly understood where and when the components of the caps are first assembled...
October 20, 2017: Protist
https://www.readbyqxmd.com/read/29149676/high-salt-diet-decreases-reproductive-performance-in-rams-and-down-regulates-gene-expression-of-some-components-of-the-renin-angiotensin-system-in-the-testis
#2
Yi Fang, Rongzhen Zhong, Xuezhao Sun, Daowei Zhou
Although salt-tolerant plants can be used to combat dryland salinity, these plants contain high concentrations of salt (NaCl), which may have deleterious effects if fed to livestock. Twenty-four Merino rams (9 mo of age) with similar body weight were equally allocated to two groups and fed a normal- or high-salt diet (0.5 and 12% NaCl, respectively) for 3 mo. Rams fed the high-salt diet had lower live weight gains, higher water intake, smaller testes (466 ± 48.4 vs 604 ± 51.1 g) and reductions (P <0...
November 10, 2017: Theriogenology
https://www.readbyqxmd.com/read/29149600/repair-of-uv-induced-dna-damage-independent-of-nucleotide-excision-repair-is-masked-by-mutyh
#3
Abdelghani Mazouzi, Federica Battistini, Sarah C Moser, Joana Ferreira da Silva, Marc Wiedner, Michel Owusu, Charles-Hugues Lardeau, Anna Ringler, Beatrix Weil, Jürgen Neesen, Modesto Orozco, Stefan Kubicek, Joanna I Loizou
DNA lesions caused by UV damage are thought to be repaired solely by the nucleotide excision repair (NER) pathway in human cells. Patients carrying mutations within genes functioning in this pathway display a range of pathologies, including an increased susceptibility to cancer, premature aging, and neurological defects. There are currently no curative therapies available. Here we performed a high-throughput chemical screen for agents that could alleviate the cellular sensitivity of NER-deficient cells to UV-induced DNA damage...
November 16, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29149593/notch2-hajdu-cheney-mutations-escape-scf-fbw7-dependent-proteolysis-to-promote-osteoporosis
#4
Hidefumi Fukushima, Kouhei Shimizu, Asami Watahiki, Seira Hoshikawa, Tomoki Kosho, Daiju Oba, Seiji Sakano, Makiko Arakaki, Aya Yamada, Katsuyuki Nagashima, Koji Okabe, Satoshi Fukumoto, Eijiro Jimi, Anna Bigas, Keiichi I Nakayama, Keiko Nakayama, Yoko Aoki, Wenyi Wei, Hiroyuki Inuzuka
Hajdu-Cheney syndrome (HCS), a rare autosomal disorder caused by heterozygous mutations in NOTCH2, is clinically characterized by acro-osteolysis, severe osteoporosis, short stature, neurological symptoms, cardiovascular defects, and polycystic kidneys. Recent studies identified that aberrant NOTCH2 signaling and consequent osteoclast hyperactivity are closely associated with the bone-related disorder pathogenesis, but the exact molecular mechanisms remain unclear. Here, we demonstrate that sustained osteoclast activity is largely due to accumulation of NOTCH2 carrying a truncated C terminus that escapes FBW7-mediated ubiquitination and degradation...
November 16, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29149545/mechanical-properties-in-metal-organic-frameworks-emerging-opportunities-and-challenges-for-device-functionality-and-technological-applications
#5
REVIEW
Nicholas C Burtch, Jurn Heinen, Thomas D Bennett, David Dubbeldam, Mark D Allendorf
Some of the most remarkable recent developments in metal-organic framework (MOF) performance properties can only be rationalized by the mechanical properties endowed by their hybrid inorganic-organic nanoporous structures. While these characteristics create intriguing application prospects, the same attributes also present challenges that will need to be overcome to enable the integration of MOFs with technologies where these promising traits can be exploited. In this review, emerging opportunities and challenges are identified for MOF-enabled device functionality and technological applications that arise from their fascinating mechanical properties...
November 17, 2017: Advanced Materials
https://www.readbyqxmd.com/read/29149540/controllable-synthesis-of-organic-microcrystals-with-tunable-emission-color-and-morphology-based-on-molecular-packing-mode
#6
Zhi-Zhou Li, Liang-Sheng Liao, Xue-Dong Wang
Organic microcrystals are of essential importance for high fluorescence efficiency, ordered molecular packing mode, minimized defects, and smooth shapes, which are extensively applied in organic optoelectronics. The molecular packing mode significantly influences the optical/electrical properties of organic microcrystals, which makes the controllable preparation of organic microcrystals with desired molecular packing mode extremely important. In the study, yellow-emissive α phase organic microcrystals with rectangular morphology and green-emissive β phase perylene microcrystals with rhombic morphology are separately prepared by simply controlling the solution concentration...
November 17, 2017: Small
https://www.readbyqxmd.com/read/29149533/monocyte-specific-knockout-of-c-ebp%C3%AE-results-in-osteopetrosis-phenotype-blocks-bone-loss-in-ovariectomized-mice-and-reveals-an-important-function-of-c-ebp%C3%AE-in-osteoclast-differentiation-and-function
#7
Wei Chen, Guochun Zhu, Joel Jules, Diep Nguyen, Yi-Ping Li
CCAAT/enhancer-binding protein α (C/ebpα) is critical for osteoclastogenesis by regulating osteoclast (OC) lineage commitment and is also important for OC differentiation and function in vitro. However, the role of C/ebpα in postnatal skeletal development has not been reported owing to lethality in C/ebpα(-/-) mice from hypoglycemia within 8 hours after birth. Herein, we generated conditional knockout mice by deleting the C/ebpα gene in monocyte via LysM-Cre to examine its role in OC differentiation and function...
November 17, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29149484/comparative-analysis-of-proteomes-between-diabetic-and-normal-human-sperm-insights-into-the-effects-of-diabetes-on-male-reproduction-based-on-the-regulation-of-mitochondria-related-proteins
#8
Tian An, Yue-Fen Wang, Jia-Xian Liu, Yan-Yun Pan, Yu-Fei Liu, Zhong-Chen He, Fang-Fang Mo, Jun Li, Li-Hua Kang, Yu-Jie Gu, Bo-Han Lv, Si-Huan Gao, Guang-Jian Jiang
This study sought to identify sources of the reduced fertility of men with type 2 diabetes mellitus. Significant reductions in semen volume, sperm concentration, and total sperm count were observed in diabetic individuals, while transmission electron microscopy revealed that the structure of mitochondria in the tail of sperm from diabetic patients was damaged. Proteins potentially associated with these sperm defects were identified using proteomics. Isobaric tagging for relative and absolute quantitation labeling and high-performance liquid chromatography-tandem mass spectrometry allowed us to identify 357 proteins significantly differentially expressed in diabetic versus control semen (>1...
November 17, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29149408/function-of-translationally-controlled-tumor-protein-in-organ-growth-lessons-from-drosophila-studies
#9
Kwang-Wook Choi, Sung-Tae Hong, Thao Phuong Le
Regulation of cell growth and proliferation is crucial for development and function of organs in all animals. Genetic defects in growth control can lead to developmental disorders and cancers. Translationally controlled tumor protein (TCTP) is a family of evolutionarily conserved proteins implicated in cancer. Recent studies have revealed multiple roles of TCTP in diverse cellular events, but TCTP functions in vivo are poorly understood in vertebrate systems. We have used Drosophila melanogaster, the fruit fly, as a model organism for genetic dissection of Tctp function...
2017: Results and Problems in Cell Differentiation
https://www.readbyqxmd.com/read/29149299/twj-screen-an-isothermal-screening-assay-to-assess-ligand-dna-junction-interactions-in-vitro
#10
Ludivine Guyon, Marc Pirrotta, Katerina Duskova, Anton Granzhan, Marie-Paule Teulade-Fichou, David Monchaud
The quest for chemicals able to operate at selected genomic loci in a spatiotemporally controlled manner is desirable to create manageable DNA damages. Mounting evidence now shows that alternative DNA structures, including G-quadruplexes and branched DNA (or DNA junctions), might hamper proper progression of replication fork, thus triggering DNA damages and genomic instability. Therefore, small molecules that stabilize these DNA structures are currently scrutinized as a promising way to create genomic defects that cannot be dealt with properly by cancer cells...
November 15, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29149261/glut10-maintains-the-integrity-of-major-arteries-through-regulation-of-redox-homeostasis-and-mitochondrial-function
#11
Yu-Wei Syu, Hao-Wen Lai, Chung-Lin Jiang, Hong-Yuan Tsai, Chung-Chih Lin, Yi-Ching Lee
Glucose transporter 10 (GLUT10) is a member of the GLUT family of membrane transporters, and mutations in this gene cause arterial tortuosity syndrome (ATS). However, the physiological role and regulation of GLUT10 in arteries remains unclear. To further understand its physiological roles in major arteries, we examined the regulatory mechanisms of GLUT10 in ASMCs and aortic tissues. Interestingly, we find that targeting of GLUT10 to mitochondria is increased in ASMCs under both stress and aging conditions, which enhances dehydroascorbic acid (DHA) uptake and maintains intracellular ascorbic acid (AA) levels...
November 15, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/29149195/age-of-heart-disease-presentation-and-dysmorphic-nuclei-in-patients-with-lmna-mutations
#12
Jason Q Core, Mehrsa Mehrabi, Zachery R Robinson, Alexander R Ochs, Linda A McCarthy, Michael V Zaragoza, Anna Grosberg
Nuclear shape defects are a distinguishing characteristic in laminopathies, cancers, and other pathologies. Correlating these defects to the symptoms, mechanisms, and progression of disease requires unbiased, quantitative, and high-throughput means of quantifying nuclear morphology. To accomplish this, we developed a method of automatically segmenting fluorescently stained nuclei in 2D microscopy images and then classifying them as normal or dysmorphic based on three geometric features of the nucleus using a package of Matlab codes...
2017: PloS One
https://www.readbyqxmd.com/read/29149049/preparation-of-solution-blown-polyamic-acid-nanofibers-and-their-imidization-into-polyimide-nanofiber-mats
#13
Jing Li, Guocheng Song, Junrong Yu, Yan Wang, Jing Zhu, Zuming Hu
Solution blow spinning (SBS) is an innovative process for spinning micro/nanofibers. In this paper, polyamic acid (PAA) nanofibers were fabricated via a SBS apparatus and then imidized into polyimide (PI) nanofibers via thermal process. The morphology and diameter distributions of PAA nanofibers were determined by scanning electron microscope (SEM) and Image Tool software, the processing parameters, including PAA concentration, solution feeding rate, gas pressure, nozzle size, and receiving distance were investigated in details...
November 17, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29149017/the-ms6-mycolyl-arabinogalactan-esterase-lysb-is-essential-for-an-efficient-mycobacteriophage-induced-lysis
#14
Adriano M Gigante, Cheri M Hampton, Rebecca S Dillard, Filipa Gil, Maria João Catalão, José Moniz-Pereira, Elizabeth R Wright, Madalena Pimentel
All dsDNA phages encode two proteins involved in host lysis, an endolysin and a holin that target the peptidoglycan and cytoplasmic membrane, respectively. Bacteriophages that infect Gram-negative bacteria encode additional proteins, the spanins, involved in disruption of the outer membrane. Recently, a gene located in the lytic cassette was identified in the genomes of mycobacteriophages, which encodes a protein (LysB) with mycolyl-arabinogalactan esterase activity. Taking in consideration the complex mycobacterial cell envelope that mycobacteriophages encounter during their life cycle, it is valuable to evaluate the role of these proteins in lysis...
November 17, 2017: Viruses
https://www.readbyqxmd.com/read/29148814/high-yield-production-of-mos2-and-ws2-quantum-sheets-from-their-bulk-materials
#15
Chunchun Han, Yong Zhang, Peng Gao, Shulin Chen, Xinfeng Liu, Yang Mi, Jianqi Zhang, Yanhong Ma, Wenyu Jiang, Jinquan Chang
Mass production of two-dimensional quantum sheets (2D QSs) is highly desired to fully exploit their properties. Herein, we present a general strategy for the high-yield production of molybdenum disulfide (MoS2) and tungsten disulfide (WS2) QSs by a sequential combination of salt-assisted ball-milling and sonication-assisted solvent exfoliation of their bulk materials. Such strategy enables reproducible production of intrinsic and defect-free MoS2 and WS2 QSs with exceedingly high yield of 25.5 wt% and 20.1 wt% respectively...
November 17, 2017: Nano Letters
https://www.readbyqxmd.com/read/29148792/proteomic-markers-of-the-functional-sperm-population-in-bovines-1-comparison-of-low-and-high-density-spermatozoa-following-cryopreservation
#16
Olivier D'Amours, Gilles Frenette, Sylvie Bourassa, Ezequiel Calvo, Patrick Blondin, Robert Sullivan
Mammalian semen contains a heterogeneous population of sperm cells. This heterogeneity results from variability in the complex processes of cell differentiation in the testis, biochemical modifications undergone by spermatozoa during transit along the male reproductive tract, interactions with secretions from accessory sex glands at ejaculation, and, in the context of reproductive technologies, in the ability of ejaculated spermatozoa to resist damage associated with freeze-thaw procedures. When submitted to density gradient centrifugation, ejaculated spermatozoa distribute themselves into two distinct populations: a low-density population characterized by low motility parameters, and a high-density population with high motility characteristics...
November 17, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29148784/multistage-transformation-and-lattice-fluctuation-at-agcl-ag-interface
#17
Jingshan S Du, Jungwon Park, QHwan Kim, Wonho Jhe, Vinayak P Dravid, Deren Yang, David A Weitz
Solid-state transformation between different materials is often accompanied by mechanical expansion and compression due to their volume change and structural evolution at interfaces. However, these two types of dynamics are usually difficult to monitor in the same time. In this work, we use in situ transmission electron microscopy to directly study the reduction transformation at the AgCl-Ag interface. Three stages of lattice fluctuations were identified and correlated to the structural evolution. During the steady state, a quasi-layered growth mode of Ag in both vertical and lateral directions were observed due to the confinement of AgCl lattices...
November 17, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29148718/atomic-layer-deposition-of-single-crystalline-beo-epitaxially-grown-on-gan-substrates
#18
Seung Min Lee, Jung Hwan Yum, Seonno Yoon, Eric S Larsen, Woo Chul Lee, Seong Keun Kim, Shahab Shervin, Weijie Wang, Jae-Hyun Ryou, Christopher W Bielawski, Jungwoo Oh
We have developed a beryllium oxide (BeO) thin film process by atomic layer deposition (ALD) and grown a single-crystal BeO on a gallium nitride (GaN) substrate for the first time. BeO has a higher thermal conductivity than all other metals except Cu, Ag, and Au, and it has a higher bandgap energy and dielectric constant than SiO2 and Al2O3. As an electrical insulator, diamond is the only material on Earth whose thermal conductivity exceeds that of BeO. Despite these advantages, there is no chemical vapor deposition technique for BeO thin film deposition, and thus, it is not used in nanoscale semiconductor device processing...
November 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29148704/electrospun-poly-3-hydroxybutyrate-co-4-hydroxybutyrate-graphene-oxide-scaffold-enhanced-properties-and-promoted-in-vivo-bone-repair-in-rats
#19
Tengfei Zhou, Guo Li, Shiyu Lin, Taoran Tian, Quanquan Ma, Qi Zhang, Sirong Shi, Changyue Xue, Wenjuan Ma, Xiaoxiao Cai, Yunfeng Lin
Bone tissue engineering emerges as an advantageous technique to achieve tissue regeneration. Scaffolds for it must present excellent biomechanical properties where bare polymers poorly perform. Development for new biomaterials with high osteogenic capacity is urgently pursued. In this study, an electrospun poly(3-hydroxybutyrate-co-4-hydroxybutyrate/graphene oxide (P34HB/GO) nanofibrous scaffold is successfully fabricated and characterized. The effects of GO amount on scaffold morphology, biomechanical properties, and cellular behaviors are investigated...
November 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29148594/metabolic-study-of-paeoniflorin-and-total-paeony-glucosides-from-paeoniae-radix-rubra-in-rats-by-high-performance-liquid-chromatography-coupled-with-sequential-mass-spectrometry-uplc-esi-ms-n
#20
Lijun Zhu, Shanshan Sun, Yanxi Hu, Yufeng Liu
A clear understanding of the metabolism of Traditional Chinese Medicine (TCM) is extremely important in their rational clinical application and effective material foundation research. A novel and reliable strategy was performed to find more metabolites of paeoniflorin, determine the metabolites of total paeony glucosides (TPG) by means of determining those metabolites of paeoniflorin, and compare the metabolism differences between paeoniflorin and TPG by intragastric administration. This strategy was characterized as follows: firstly, the rats were divided into two groups (the paeoniflorin group and the TPG group) to find different metabolism mechanism between paeoniflorin and TPG; secondly, UPLC-FT-ICR MS and UPLC-Q-TOF MS(2) were applied to obtain accurate molecular weight and structural information respectively; thirdly, the metabolites were tentatively identified by a combination of data-processing methods including mass defect screening, characteristic neutral loss screening and product ion screening; finally, a comparative study was employed in the metabolism of paeoniflorin and TPG...
November 17, 2017: Biomedical Chromatography: BMC
keyword
keyword
1156
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"