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Veit Rothhammer, Davis M Borucki, Emily C Tjon, Maisa C Takenaka, Chun-Cheih Chao, Alberto Ardura-Fabregat, Kalil Alves de Lima, Cristina Gutiérrez-Vázquez, Patrick Hewson, Ori Staszewski, Manon Blain, Luke Healy, Tradite Neziraj, Matilde Borio, Michael Wheeler, Loic Lionel Dragin, David A Laplaud, Jack Antel, Jorge Ivan Alvarez, Marco Prinz, Francisco J Quintana
Microglia and astrocytes modulate inflammation and neurodegeneration in the central nervous system (CNS)1-3 . Microglia modulate pro-inflammatory and neurotoxic activities in astrocytes, but the mechanisms involved are not completely understood4,5 . Here we report that TGFα and VEGF-B produced by microglia regulate the pathogenic activities of astrocytes in the experimental autoimmune encephalomyelitis (EAE) mouse model of multiple sclerosis. Microglia-derived TGFα acts via the ErbB1 receptor in astrocytes to limit their pathogenic activities and EAE development...
May 16, 2018: Nature
Xiuan Xu, Yuling Yan, Fang Liu, Lina Wu, Mengping Shao, Kai Wang, Xilin Sun, Yingbo Li, Ernest Sanyare Warmann Beinpuo, Baozhong Shen
BACKGROUND: Folate receptors (FRs) hold great potential as important diagnostic and prognostic biological marker for cancer. PURPOSE: To assess the targeted capability of the FR-targeted perfluorocarbon (PFC) nanoparticles and to assess in vivo the relationship between FR expression and tumor proliferation with fluorine-19 (19 F) MR molecular imaging. STUDY TYPE: Prospective animal cancer model. ANIMAL MODEL: H460 (n = 14) and A549 (n = 14) nude mice subcutaneous tumor models...
May 13, 2018: Journal of Magnetic Resonance Imaging: JMRI
Michiko Yamashita, Keiichi Kumasawa, Hitomi Nakamura, Tadashi Kimura
No abstract text is available yet for this article.
May 9, 2018: Biochemical and Biophysical Research Communications
Yan Xing, Tiantian Wang, Lilei Guo, Na Sun, Jinhua Zhao
Non-small cell lung cancer (NSCLC) accounts for more than 80% of lung cancer. Nowadays, gemcitabine and cisplatin in combination have been adopted as the first-line chemotherapy for patients with NSCLC. This study aimed to monitor early response to combined chemotherapy of gemcitabine plus cisplatin in a mouse model of NSCLC by using 18 F-fluorodeoxyglucose and 18 F-fluorothymidine small animal positron emission tomography (PET). Lewis lung carcinoma-bearing C57BL/6 mice were treated with gemcitabine-cisplatin or saline...
June 1, 2017: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
Fabio Cattaneo, Martina Castaldo, Melania Parisi, Raffaella Faraonio, Gabriella Esposito, Rosario Ammendola
In the vasculature, NADPH oxidase is the main contributor of reactive oxygen species (ROS) which play a key role in endothelial signalling and functions. We demonstrate that ECV304 cells express p47phox , p67phox , and p22phox subunits of NADPH oxidase, as well as formyl peptide receptors 1 and 3 (FPR1/3), which are members of the GPCR family. By RT-PCR, we also detected Flt-1 and Flk-1/KDR in these cells. Stimulation of FPR1 by N-fMLP induces p47phox phosphorylation, which is the crucial event for NADPH oxidase-dependent superoxide production...
2018: Oxidative Medicine and Cellular Longevity
Takayasu Ohtake, Shuzo Kobayashi, Shimon Slavin, Yasuhiro Mochida, Kunihiro Ishioka, Hidekazu Moriya, Sumi Hidaka, Ryo Matsuura, Maki Sumida, Daisuke Katagiri, Eisei Noiri, Kayoko Okada, Hiroshi Mizuno, Rica Tanaka
Acute kidney injury (AKI) is a major clinical problem that still has no established treatment. We investigated the efficacy of cultured human peripheral blood mononuclear cells (PBMNCs) for AKI. Ischemia/reperfusion injury (IRI) was used to induce AKI in male nonobese diabetic (NOD/severe combined immunodeficiency) mice aged 7 to 8 wk. PBMNCs were isolated from healthy volunteers and were subjected to quality and quantity controlled (QQc) culture for 7 d in medium containing stem cell factor, thrombopoietin, Flt-3 ligand, vascular endothelial growth factor, and interleukin 6...
January 1, 2018: Cell Transplantation
Mahmoud Ali Abdelaziz Mahmoud, Rafat Milad Mohareb, Mashari Ahmed Alshereef
BACKGROUND: Among wide range of heterocyclic steroidal derivatives acquired a special attention due to their wide range of pharmacological activities especially the therapeutic activities. Many pharmacological drugs containing the steroid nucleus were known in the market. OBJECTIVE: Our main aim of this work was to synthesis a seies of heterocyclic compounds especially thiophene and thienopyridine derivatives containing the estrone nucleuous. The synthesized compounds possising antitumor and kinases inhibitions...
May 7, 2018: Anti-cancer Agents in Medicinal Chemistry
Hakimeh Mohammadi, Eszter Papp, Lindsay Cahill, Monique Rennie, Nicole Banko, Lakmini Pinnaduwage, Janice Lee, Mark Kibschull, Caroline Dunk, John G Sled, Lena Serghides
Adverse birth outcomes are common in HIV-positive pregnant women receiving combination antiretroviral therapy (cART), especially when cART is initiated in early pregnancy. The mechanisms remain poorly understood. Using a mouse model we demonstrate that protease inhibitor based-cART exposure beginning on day 1 of pregnancy was associated with a pro-angiogenic/pro-branching shift in the placenta driven by lower Flt-1 levels and higher Gcm-1 expression. Micro-CT imaging revealed an increase in the number of arterioles in cART-treated placentas, which correlated with fetal growth restriction...
April 26, 2018: Scientific Reports
B M Mendes, A V Ferreira, L T C Nascimento, S M Z M D Ferreira, M B Silveira, J B Silva
3'-Deoxy-3-[18 F]fluorothymidine, or [18 F]FLT, is a positron emission tomography (PET) tracer used in clinical studies for noninvasive assessment of proliferation activity in several types of cancer. Although the use of this PET tracer is expanding, to date, few studies concerning its dosimetry have been published. In this work, new [18 F]FLT dosimetry estimates are determined for human and mice using Monte Carlo simulations. Modern voxelized male and female phantoms and [18 F]FLT biokinetic data, both published by the ICRP, were used for simulations of human cases...
April 25, 2018: Radiation Research
Lindsey B Crawford, Jung Heon Kim, Donna Collins-McMillen, Byeong-Jae Lee, Igor Landais, Christine Held, Jay A Nelson, Andrew D Yurochko, Patrizia Caposio
The ability of human cytomegalovirus (HCMV) to reactivate from latent infection of hematopoietic progenitor cells (HPCs) is intimately linked to cellular differentiation. HCMV encodes UL7 that our group has shown is secreted from infected cells and induces angiogenesis. In this study, we show that UL7 is a ligand for Fms-like tyrosine kinase 3 receptor (Flt-3R), a well-known critical factor in HPC differentiation. We observed that UL7 directly binds Flt-3R and induces downstream signaling cascades, including phosphatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathways...
April 24, 2018: MBio
S Sentürklü, D G Landblom, R Maddock, T Petry, C J Wachenheim, S I Paisley
In a 2-yr study, spring-born yearling steers (n = 144), previously grown to gain < 0.454 kg·steer-1·d-1, following weaning in the fall, were stratified by BW and randomly assigned to three retained ownership rearing systems (3-replications) in early May. Systems were: 1) feedlot (FLT), 2) steers that grazed perennial crested wheatgrass (CWG) and native range (NR) before feedlot entry (PST), and 3) steers that grazed perennial CWG and NR, and then field pea-barley mix (PBLY) and unharvested corn (UC) before feedlot entry (ANN)...
April 24, 2018: Journal of Animal Science
Christopher McHugh, Monica R Thipparthi, Jawana M Lawhorn-Crews, Lisa Polin, Shirish Gadgeel, Janice Akoury, Thomas J Mangner, Kirk A Douglas, Jing Li, Manohar Ratnam, Anthony F Shields
Non-small cell lung cancer (NSCLC) is a leading cause of cancer mortality in the United States and pemetrexed-based therapies are regularly used to treat non-squamous NSCLC. Despite widespread use, pemetrexed has a modest effect on progression-free survival, with varying efficacy between individuals. Recent work has indicated that dexamethasone, given to prevent pemetrexed toxicity, is able to protect a subset of NSCLC cells from pemetrexed cytotoxicity by temporarily suppressing of the S-phase of the cell cycle...
April 19, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Yun Zhang, Yong-Gang Zhao, Wei-Sheng Chen, He-Li Cheng, Xiu-Qiong Zeng, Yan Zhu
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitously found in the environment and have been proved to be prospectively associated with the risk of cancer. In this study, a simple method based on pipette-tip solid phase extraction (PT-SPE) and gas chromatography-mass spectrometry (GC-MS) has been firstly developed for the determination of 16 PAHs in human whole blood. Three-dimensional ionic liquid-ferrite functionalized graphene oxide nanocomposite (3D-IL-Fe3 O4 -GO) was used as sorbent in PT-SPE. Compared with conventional SPE method, the PT-SPE method was solvent-saving (1...
March 21, 2018: Journal of Chromatography. A
Karin Zattar Cecyn, Eliza Y S Kimura, Dulce Marta S M Lima, Miyoko Yamamoto, José Orlando Bordin, José Salvador R de Oliveira
Background: Cell adhesion molecules (CAMs) expressed on hematopoietic progenitor cells (HPCs), endothelial cells, and stromal cells play a pivotal role in the mobilization of CD34+ cells. Herein, we conducted a non-randomized peripheral blood stem cell (PBSC) mobilization study aimed to compare the potential differences in the expressions of several CAMs and chemokines on CD34+ cells obtained from bone marrow aspirate before and after HPC mobilization from patients with hematologic malignancies and healthy donors...
March 2018: Blood Research
Juliann G Kiang, Joan T Smith, Sara R Hegge, Natalia I Ossetrova
Exposure to ionizing radiation is a crucial life-threatening factor in nuclear and radiological incidents. It is known that ionizing radiation affects cytokine/chemokine concentrations in the blood of B6D2F1 mice. It is not clear whether radiation dose rates would vary the physiological response. Therefore, in this study we utilized data from two experiments using B6D2F1 female mice exposed to six different dose rates ranging from low to high rates. In one experiment, mice received a total dose of 8 Gy (LD0/30 ) of 60 Co gamma radiation at four dose rates: 0...
April 13, 2018: Radiation Research
Francesca Iommelli, Viviana De Rosa, Cristina Terlizzi, Marcello Monti, Mariarosaria Panico, Rosa Fonti, Silvana Del Vecchio
PURPOSE: Our aim was to test whether imaging with 18 F-fluorothymidine (18 F-FLT) PET/CT was able to detect the combined effects of EGFR and MET inhibitors in oncogene-driven non-small lung cancer (NSCLC) and to elucidate the mechanisms underlying the enhanced efficacy of drug combination. EXPERIMENTAL DESIGN: NSCLC cells bearing MET amplification (H1993 and H820) were treated with EGFR and MET inhibitors either alone or in combination and then tested for cell viability and inhibition of signaling...
April 4, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
R Iqbal, G M Kramer, V Frings, E F Smit, O S Hoekstra, R Boellaard
BACKGROUND: 3'-Deoxy-3'-[18 F]fluorothymidine ([18 F]FLT) was proposed as an imaging biomarker for the assessment of in vivo cellular proliferation with positron emission tomography (PET). The current study aimed to validate [18 F]FLT as a perfusion-independent PET tracer, by gaining insight in the intra-tumoural relationship between [18 F]FLT uptake and perfusion in non-small cell lung cancer (NSCLC) patients undergoing treatment with a tyrosine kinase inhibitor (TKI). Six patients with metastatic NSCLC, having an activating epidermal growth factor receptor (EGFR) mutation, were included in this study...
March 27, 2018: EJNMMI Research
Hai-Long Xu, Man Li, Rong-Jun Zhang, Hui-Jie Jiang, Ming-Yu Zhang, Xin Li, Yi-Qiao Wang, Wen-Bin Pan
BACKGROUND: Positron emission tomography (PET) is a noninvasive method to characterize different metabolic activities of tumors, providing information for staging, prognosis, and therapeutic response of patients with cancer. The aim of this study was to evaluate the feasibility of18 F-fludeoxyglucose (18 F-FDG) and 3'-deoxy-3'-18 F-fluorothymidine (18 F-FLT) PET in predicting tumor biological characteristics of colorectal cancer liver metastasis. METHODS: The uptake rate of18 F-FDG and18 F-FLT in SW480 and SW620 cells was measured via an in vitro cell uptake assay...
March 13, 2018: Hepatobiliary & Pancreatic Diseases International: HBPD INT
HongYing Mei, Wen Xu, Chao Wang, HaiFeng Yuan, Chao Zhang, Lan Ding, Jin Zhang, Chao Deng, YiFan Wang, Francois M Peeters
Magneto-optical (MO) properties of bi- and tri-layer graphene are investigated utilizing terahertz time-domain spectroscopy (THz TDS) in the presence of a strong magnetic fleld at room-temperature. In the Faraday conflguration and applying optical polarization measurements, we measure the real and imaginary parts of the longitudinal and transverse MO conductivities of difierent graphene samples. The obtained experimental data flt very well with the well-known MO Drude formula, from which we obtain key sample and material parameters of bi- and tri-layer graphene, such as the electron efiective mass, the&#13; electronic relaxation time and the electron density...
March 20, 2018: Journal of Physics. Condensed Matter: An Institute of Physics Journal
R M Wang, B X Xu, C B Liu, Z W Guan, L P Fu, J M Zhang, F Li, L Sun, H Y Zhu
No abstract text is available yet for this article.
January 14, 2018: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
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