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Wei Wei, Chao Xu, Zhi-Yong Ye, Xiao-Jun Huang, Jia-En Yuan, Tian-Bao Ma, Han-Biao Lin, Xiu-Qiong Chen
The aim of the present study was to obtain the qualified hematopoietic stem/progenitor cells (HSC/HPC) and human umbilical cord-mesenchymal stem cells (MSC) in vitro in the co-culture system. Cord blood mononuclear cells were separated from umbilical cord blood by Ficoll lymphocyte separation medium, and then CD34(+) HSC was collected by MACS immunomagnetic beads. The selected CD34(+) HSC/HPC and MSC were transferred into culture flask. IMDM culture medium with 15% AB-type cord plasma supplemented with interleukin-3 (IL-3), IL-6, thrombopoietin (TPO), stem cell factor (SCF) and FMS-like tyrosine kinase 3 ligand (Flt-3L) factors were used as the co-culture system for the amplification of HSC/HPC and MSC...
October 25, 2016: Sheng Li Xue Bao: [Acta Physiologica Sinica]
Gilad Yahav, Eran Barnoy, Nir Roth, Lior Turgeman, Dror Fixler
Fluorescence lifetime imaging microscopy (FLIM) is an essential tool in many scientific fields such as biology and medicine thanks to the known advantages of the fluorescence lifetime (FLT) over the classical fluorescence intensity (FI). However, the frequency domain (FD) FLIM technique suffers from its strong dependence on the reference and its compliance to the sample. In this paper, we suggest a new way to calculate the FLT by using the crossing point (CRPO) between the modulation and phase FLTs measured over several light emitting diode (LED) DC currents values instead of either method alone...
October 24, 2016: Journal of Biophotonics
Masatoyo Nakajo, Yoriko Kajiya, Megumi Jinguji, Yoshiaki Nakabeppu, Masayuki Nakajo, Tohru Nihara, Takashi Yoshiura
Positron emission tomography (PET) or PET/computed tomography (CT) using (18)F-3'-fluoro-3'-deoxythymidine ((18)F-FLT) offers noninvasive assessment of cell proliferation in human cancers in vivo. The present review discusses the current status on clinical applications of (18)F-FLT-PET (or PET/CT) in digestive and abdominal oncology by comparing with (18)F-fluorodeoxyglucose ((18)F-FDG)-PET (or PET/CT). The results of this review show that although (18)F-FLT uptake is lower in most cases of digestive and abdominal malignancies compared with (18)F-FDG uptake, (18)F-FLT-PET can be used to detect primary tumors...
October 21, 2016: Abdominal Radiology
Vinoth Lakshmanan
Flutamide (FLT) is a non steroidal antiandrogenic drug used to treat prostate cancer. Its poor aqueous solubility and toxicity are the major hindrance for oral drug delivery. The nanoformulation of flutamide increases its aqueous solubility thereby improves the therapeutic efficacy of the chemodrug. Poly (vinyl alcohol) (PVA) coated flutamide nanoparticles (PVA FLT NPs) were formulated by nanoprecipitation method and characterized by DLS, TEM and FTIR. The nanoparticles were about 300nm size and spherical in shape...
October 18, 2016: Current Drug Delivery
Yao-Lung Chang, An-Shine Chao, Shuenn-Dyh Chang, Peter Cc Hsieh, Sheng-Yuan Su, Kuan-Ju Chen, Po-Jen Cheng, Tzu-Hao Wang
OBJECTIVE: To evaluate the outcome of twin-twin transfusion syndrome (TTTS) treated by fetoscopic laser therapy (FLT) stratified by Quintero staging. MATERIALS AND METHODS: A total of 100 TTTS cases treated by FLT, from October 2005 to August 2014, were included in this study. Cases were divided into first and second half periods to evaluate the learning effect-related outcomes, and logistic regression was applied to determine the independent factors in predicting the perinatal outcomes...
October 2016: Taiwanese Journal of Obstetrics & Gynecology
Christopher I McHugh, Jawana M Lawhorn-Crews, Dipenkumar Modi, Kirk A Douglas, Steven K Jones, Thomas J Mangner, Jerry M Collins, Anthony F Shields
BACKGROUND: A principal goal for the use of positron emission tomography (PET) in oncology is for real-time evaluation of tumor response to chemotherapy. Given that many contemporary anti-neoplastic agents function by impairing cellular proliferation, it is of interest to develop imaging modalities to monitor these pathways. Here we examined the effect of capecitabine on the uptake of thymidine analogs used with PET: 3'-deoxy-3'-[(18)F]fluorothymidine ((18)F-FLT), 1-(2'-deoxy-2'-[(18)F]fluoro-β-D-arabinofuranosyl) thymidine ((18)F-FMAU), and 1-(2'-deoxy-2'-[(18)F]fluoro-β-D-arabinofuranosyl) uracil ((18)F-FAU) in patients with advanced cancer...
October 17, 2016: Cancer Imaging: the Official Publication of the International Cancer Imaging Society
Prabhu Thirusangu, V Vigneshwaran, T Prashanth, B R Vijay Avin, Vikas H Malojirao, H Rakesh, Shaukath Ara Khanum, Riaz Mahmood, B T Prabhakar
Hypoxia is a feature of all solid tumours, contributing to tumour progression. Activation of HIF-1α plays a critical role in promoting tumour angiogenesis and metastasis. Since its expression is positively correlated with poor prognosis for cancer patients, HIF-1α is one of the most convincing anticancer targets. BP-1T is a novel antiproliferative agent with promising antiangiogenic effects. In the present study, the molecular mechanism underlying cytotoxic/antiangiogenic effects of BP-1T on tumour/non-tumour angiogenesis was evaluated...
October 14, 2016: Angiogenesis
Shang-You Yang, Nora Strong, Xuan Gong, Michael H Heggeness
BACKGROUND: Stem cell-involved tissue engineering has gained dramatic attention as a therapeutic strategy for tissue regeneration including bone repair. However, the currently available possibilities to use embryonic stem cells and induced pluripotent stem cells (iPCs) face potential ethical issues, as well as risks of malignant transformation and immune rejection. Recently identified peripheral nerve-derived adult pluripotent cells (NEDAPS) that quickly proliferate after exposure to BMP-2 or nerve trauma and exhibit many embryonic stem cell characteristics may provide an attractive source cells for a variety of regenerative therapies...
October 10, 2016: Spine Journal: Official Journal of the North American Spine Society
Chun-Yi Wu, Jo-Hsin Tang, Pei-Chia Chan, Jia-Je Li, Ming-Hsien Lin, Chih-Chieh Shen, Ren-Shyan Liu, Hsin-Ell Wang
PURPOSE: Surgical resection is the standard treatment for localized colorectal cancer, which is the most common type of gastrointestinal cancer. However, over 40 % cases are diagnosed metastasized and apparently inoperable. Systemic chemotherapy provides an alternative to these patients. This study aims to evaluate the therapeutic potential of liposomal doxorubicin (lipoDox) in combination with liposomal vinorelbine (lipoVNB) in a CT-26 colon carcinoma-bearing mouse model. PROCEDURES: The in vitro cytotoxicity of Dox and VNB on CT-26 cancer cells was determined by MTT and colony formation assays...
October 11, 2016: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Andrea Maria Mess, Ana Claudia Oliveira Carreira, Cláudia Marinovic de Oliveira, Paula Fratini, Phelipe Oliveira Favaron, Rodrigo da Silva Nunes Barreto, Christiane Pfarrer, Flávio Vieira Meirelles, Maria Angelica Miglino
Reproductive technologies are widely used in cattle, although many are associated with high-embryonic mortality, especially during early gestation, when the yolk sac undergoes macroscopic changes in structure. We hypothesized that vasculogenesis and angiogenesis are affected, thereby affecting embryonic and placental differentiation. To test this, we studied yolk sac development and gene expression of the vascular endothelial growth factor system (VEGF-A, VEGFR-1/Flt-1, VEGFR-2/KDR). Samples from Days 25 to 40/41 of pregnancy from control cattle (n = 8) and from pregnancies established with IVF, (n = 7) or somatic cell nuclear transfer/clones (n = 5) were examined by histology, immunohistochemistry, and quantitative reverse transcriptase PCR...
September 13, 2016: Theriogenology
Tamar Zahavi, Gilad Yahav, Yael Shimshon, Sivan Gershanov, Luna Kaduri, Amir Sonnenblick, Dror Fixler, Asher Y Salmon, Mali Salmon-Divon
Worldwide, more than one million women are diagnosed with breast cancer every year, making it the most common female malignancy in the developed world. Germline mutations in BRCA1 and BRCA2 genes are estimated to increase the risk for developing breast cancer by up to 87%. From a clinical point of view, identification of BRCA1 and BRCA2 mutation carriers offers an opportunity to early identify or prevent the development of malignancy; therefore the ability to determine which women are more likely to carry BRCA1 or BRCA2 mutations is of great importance...
November 4, 2016: Biochemical and Biophysical Research Communications
Stefan Wiehr, Anna-Maria Rolle, Philipp Warnke, Ursula Kohlhofer, Leticia Quintanilla-Martinez, Gerald Reischl, Ingo B Autenrieth, Bernd J Pichler, Stella E Autenrieth
Most frequently, gram-negative bacterial infections in humans are caused by Enterobacteriaceae and remain a major challenge in medical diagnostics. We non-invasively imaged moderate and severe systemic Yersinia enterocolitica infections in mice using the positron emission tomography (PET) tracer 3'-deoxy-3'-[18F]fluorothymidine ([18F]FLT), which is a marker of proliferation, and compared the in vivo results to the ex vivo biodistributions, bacterial loads, and histologies of the corresponding organs. Y. enterocolitica infection is detectable with histology using H&E staining and immunohistochemistry for Ki 67...
2016: PloS One
Sang-Gyu Lee, Kishore Gangangari, Teja Muralidhar Kalidindi, Blesida Punzalan, Steven M Larson, Naga Vara Kishore Pillarsetty
INTRODUCTION: Bone marrow is the soft tissue compartment inside the bones made up of hematopoietic cells, adipocytes, stromal cells, phagocytic cells, stem cells, and sinusoids. While [(18)F]-FLT has been utilized to image proliferative marrow, to date, there are no reports of particle based positron emission tomography (PET) imaging agents for imaging bone marrow. We have developed copper-64 labeled liposomal formulation that selectively targets bone marrow and therefore serves as an efficient PET probe for imaging bone marrow...
August 27, 2016: Nuclear Medicine and Biology
Martin A Lodge, Matthias Holdhoff, Jeffrey P Leal, Asim K Bag, L Burt Nabors, Akiva Mintz, Glenn J Lesser, David A Mankoff, Arati S Desai, James M Mountz, Frank S Lieberman, Joy D Fisher, Serena Desideri, Xiaobu Ye, Stuart A Grossman, David Schiff, Richard L Wahl
: Quantitative (18)F-3'-fluoro-3'-deoxy-L-thymidine ((18)F-FLT) positron emission tomography (PET) has potential as a non-invasive tumor biomarker for the objective assessment of response to treatment. To guide interpretation of these quantitative data, we evaluated the repeatability of (18)F-FLT PET as part of a multi-center trial involving patients with high grade glioma. METHODS: (18)F-FLT PET was performed on 10 patients with recurrent high grade glioma at 5 different institutions within the Adult Brain Tumor Consortium trial ABTC1101...
September 29, 2016: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Katsuya Mitamura, Yuka Yamamoto, Nobuyuki Kudomi, Yukito Maeda, Takashi Norikane, Keisuke Miyake, Yoshihiro Nishiyama
BACKGROUND: The nucleoside analog 3'-deoxy-3'-(18)F-fluorothymidine (FLT) has been investigated for evaluating tumor proliferating activity in brain tumors. We evaluated FLT uptake heterogeneity using textural features from the histogram analysis in patients with newly diagnosed gliomas and examined correlation of the results with proliferative activity and patient prognosis, in comparison with the conventional PET parameters. METHODS: FLT PET was investigated in 37 patients with newly diagnosed gliomas...
September 29, 2016: Annals of Nuclear Medicine
J Scholey, M L Kirk, S M McGuire, B K Teo, L L Lin
No abstract text is available yet for this article.
October 1, 2016: International Journal of Radiation Oncology, Biology, Physics
M Hu, B Fan, J Ma, S Xue, S Zhao, Z Fu, J Yu
No abstract text is available yet for this article.
October 1, 2016: International Journal of Radiation Oncology, Biology, Physics
Christy L Osgood, Mohammed N Tantawy, Nichole Maloney, Zachary B Madaj, Anderson Peck, Elissa Boguslawski, Jennifer Jess, Jason Buck, Mary E Winn, H Charles Manning, Patrick J Grohar
Ewing sarcoma is a bone and soft-tissue tumor that depends on the activity of the EWS-FLI1 transcription factor for cell survival. Although a number of compounds have been shown to inhibit EWS-FLI1 in vitro, a clinical EWS-FLI1-directed therapy has not been achieved. One problem plaguing drug development efforts is the lack of a suitable, non-invasive, pharmacodynamic marker of EWS-FLI1 activity. Here we show that (18)F-FLT PET ((18)F- 3'-deoxy-3'-fluorothymidine positron emission tomography) reflects EWS-FLI1 activity in Ewing sarcoma cells both in vitro and in vivo...
September 27, 2016: Scientific Reports
Xiaoyu An, Jinping Liu, Na Wang, Di Wang, Liang Huang, Likun Zhang, Jie Cai, Jean-Pierre Wery, Demin Zhou, Jianfeng Zhou, Qi-Xiang Li
Engrafting a M5-AML patient bone marrow (BM) cells into immunocompromised mice (AM7577) achieved serially transferrable stable AML and eventual mortality. The disease starts at BM followed by expansion to peripherals, typical of M5 leukemogenesis, where high leukemic burden in blood is coincident with symptoms/mortality. The leukemic cells in mice have similar myeloid morphology, phenotypes and genotypes (including FLT3-ITD) as the original patient. Autocrine mechanisms of human GM-CSF/IL-3 likely support AM7577 growth in mice...
September 23, 2016: Experimental Hematology
Tarun Kumar Jain, Sampanna Jung Rayamajhi, Rajender Kumar Basher, Dheeraj Gupta, Venkata Nagarjuna Maturu, Bhagwant Rai Mittal
Lung cancer is one of the leading cancers all over the world. Positron emission tomography (PET) using 18F fluorodeoxyglucose (18F FDG) is useful for staging of the disease and decide the appropriate management. 3'-deoxy-3'-18 F-fluorothymidine (18F FLT) is a tracer being extensively evaluated currently and is said to represent tumor proliferation. Common sites of metastases from lung cancer include adrenal glands, bone, and brain. Muscle metastasis and cardiac metastasis are uncommon findings. We report a case of squamous cell carcinoma of the lung with metastases to multiple skeletal muscles and myocardium detected with both FDG and FLT PET/computed tomography (CT)...
September 2016: World Journal of Nuclear Medicine
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