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https://www.readbyqxmd.com/read/28747756/combinatorial-extracellular-matrix-microenvironments-for-probing-endothelial-differentiation-of-human-pluripotent-stem-cells
#1
Luqia Hou, Joseph J Kim, Maureen Wanjare, Bhagat Patlolla, John Coller, Vanita Natu, Trevor J Hastie, Ngan F Huang
Endothelial cells derived from human pluripotent stem cells are a promising cell type for enhancing angiogenesis in ischemic cardiovascular tissues. However, our understanding of microenvironmental factors that modulate the process of endothelial differentiation is limited. We examined the role of combinatorial extracellular matrix (ECM) proteins on endothelial differentiation systematically using an arrayed microscale platform. Human pluripotent stem cells were differentiated on the arrayed ECM microenvironments for 5 days...
July 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28747214/culturing-and-transcriptome-profiling-of-progenitor-like-colonies-derived-from-adult-mouse-pancreas
#2
Dongshen Ma, Shanshan Tang, Jing Song, Qiong Wu, Fangfang Zhang, Yun Xing, Yi Pan, Yanfeng Zhang, Jingwei Jiang, Yubin Zhang, Liang Jin
BACKGROUND: Transplantation of insulin-producing cells is considered an important diabetes therapy. Many research studies have shown that insulin-producing cells can be derived from the in-vitro cultured pancreatic colonies with self-renewal ability and multilineage potential. Even though these progenitor-like colonies have been prepared from adult pancreas cells, the efficient culture method is hardly established and regulation of the colonies is rarely known. We confirmed previously that single cells acquired from adult mouse pancreas could form cyst-like colonies in a 3D semi-solid system containing Matrigel and methylcellulose...
July 26, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28743828/tm4sf5-promotes-metastatic-behavior-of-cells-in-3d-extracellular-matrix-gels-by-reducing-dependency-on-environmental-cues
#3
Dae-Geun Song, Gyu-Ho Lee, Seo Hee Nam, Jin-Gyu Cheong, Doyoung Jeong, Seo-Jin Lee, Cheol-Ho Pan, Jae Woo Jung, Hye-Jin Kim, Jihye Ryu, Ji Eon Kim, Somi Kim, Chang Yun Cho, Min-Kyung Kang, Kyung-Min Lee, Jung Weon Lee
Transmembrane 4 L six family member 5 (TM4SF5) is highly expressed in hepatocellular carcinoma tissues and enhances migration in two-dimensional environments. Here, we investigated how TM4SF5 is involved in diverse pro-metastatic phenotypes in in vivo-like three-dimensional (3D) extracellular matrix gels. TM4SF5-positive cells aggressively formed invasive foci in 3D Matrigel, depending on TM4SF5-mediated signaling activity, cytoskeletal organization, and matrix metallopeptidase (MMP) 2-mediated extracellular remodeling, whereas TM4SF5-null cells did not...
May 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28741790/diallyl-trisulfide-inhibits-cell-migration-and-invasion-of-human-melanoma-a375-cells-via-inhibiting-integrin-facal-adhesion-kinase-pathway
#4
Hsiao-Chi Wang, Yung-Lin Chu, Shu-Chen Hsieh, Lee-Yan Sheen
Melanoma is the leading cause of death from skin disease due to its propensity for metastasis. Studies have shown that integrin-mediated focal adhesion kinase (FAK) signal pathway is implicated in cell proliferation, survival and metastasis of tumor cells. Our previous results indicated that diallyl trisulfide (DATS) provided its antimelanoma activity via inducing cell cycle arrest and apoptosis. The aim of this study was to explore DATS mediated antimetastatic effect and the corresponding mechanism in human melanoma A375 cells...
July 25, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28740980/micro-engineered-perfusable-3d-vasculatures-for-cardiovascular-diseases
#5
Nishanth Venugopal Menon, Hui Min Tay, Soon Nan Wee, King Ho Holden Li, Han Wei Hou
Vessel geometries in microengineered in vitro vascular models are important to recapitulate a pathophysiological microenvironment for the study of flow-induced endothelial dysfunction and inflammation in cardiovascular diseases. Herein, we present a simple and novel extracellular matrix (ECM) hydrogel patterning method to create perfusable vascularized microchannels of different geometries based on the concept of capillary burst valve (CBV). No surface modification is necessary and the method is suitable for different ECM types including collagen, matrigel and fibrin...
July 25, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28739737/synergistic-effects-of-sam-and-selenium-compounds-on-proliferation-migration-and-adhesion-of-hela-cells
#6
Licui Sun, Jianxin Zhang, Qiu Yang, Yang Si, Yiqun Liu, Qin Wang, Feng Han, Zhenwu Huang
BACKGROUND/AIM: To determine the antitumor activities and molecular mechanism of selenium compounds in HeLa cells. MATERIALS AND METHODS: Western blotting was used to detect ERK and AKT activation in HeLa cells induced by selenium compounds selenomethionine (SeMet), methylselenocysteine (MeSeCys) and methylseleninic acids (MeSeA). Using MTT, wound-healing and Matrigel adhesion assays, the antitumor effects of SAM and selenium compounds were evaluated in HeLa cells...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28733640/interleukin-37-promotes-angiogenesis-through-tgf-%C3%AE-signaling
#7
Mengmeng Zhao, Yongguang Hu, Jiayi Jin, Ying Yu, Shanshan Zhang, Jingjing Cao, Yuanfen Zhai, Rongbin Wei, Juanjuan Shou, Wenping Cai, Shangfeng Liu, Xiaoping Yang, Guo-Tong Xu, Jianhua Yang, David B Corry, Shao Bo Su, Xialin Liu, Tianshu Yang
IL-37 is a novel pro-angiogenic cytokine that potently promotes endothelial cell activation and pathological angiogenesis in our previous study, but the mechanisms behind the pro-angiogenic effect of IL-37 are less well understood. Extending our observations, we found that TGF-β interacts with IL-37, and potently enhances the binding affinity of IL-37 to the ALK1 receptor complex, thus allowing IL-37 to signal through ALK1 to activate pro-angiogenic responses. We further show that TGF-β and ALK1 are required in IL-37 induced pro-angiogenic response in ECs and in the mouse model of Matrigel plug and oxygen-induced retinopathy...
July 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28733141/gastrin-releasing-peptide-and-its-receptor-increase-arthritis-fibroblast-like-synoviocytes-invasiveness-through-activating-the-pi3k-akt-pathway
#8
Vanessa Schuck Clarimundo, Mirian Farinon, Renata Ternus Pedó, Vivian Oliveira Nunes Teixeira, Carolina Nör, Percio S Gulko, Ricardo Machado Xavier, Patricia Gnieslaw de Oliveira
Rheumatoid arthritis (RA) is an autoimmune disease that leads to joint destruction. The fibroblast-like synoviocytes (FLS) has a central role on the disease pathophysiology. The present study aimed to examine the role of gastrin-releasing peptide (GRP) and its receptor (GRPR) on invasive behavior of mice fibroblast-like synoviocytes (FLS), as well as to evaluate GRP-induced signaling on PI3K/AKT pathway. The expression of GRPR in FLS was investigated by immunocytochemistry, western blot (WB) and qRT-PCR. The proliferation and invasion were assessed by SRB and matrigel-transwell assay after treatment with GRP and/or RC-3095 (GRPR antagonist), and/or Ly294002 (inhibitor of PI3K/AKT pathway)...
July 18, 2017: Peptides
https://www.readbyqxmd.com/read/28732179/kgf-enhances-oral-epithelial-adhesion-and-rete-peg-elongation-via-integrins
#9
G L Sa, X P Xiong, J G Ren, J Y Wang, H F Xia, Z K Liu, S G He, Y F Zhao
Oral epithelial adhesion to the lamina propria underlies the physiologic function of the oral mucosa and contributes to resisting bacterial invasion, preventing body fluid loss, and maintaining routine chewing; thus, understanding the factors that positively influence oral epithelial adhesion is a research topic of great interest. Rete pegs contribute to oral epithelial adhesion by enlarging the contact areas, whereas integrins are the major molecules that mediate epithelial cell adhesion to the basement membrane...
July 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28727579/enhanced-anticancer-efficacy-of-histone-deacetyl-inhibitor-suberoylanilide-hydroxamic-acid-in-combination-with-a-phosphodiesterase-inhibitor-pentoxifylline-in-human-cancer-cell-lines-and-in-vivo-tumor-xenografts
#10
Saranya Nidhyanandan, Boreddy S Thippeswamy, Kottapalli B Chandrasekhar, Neetinkumar D Reddy, Nagaraj M Kulkarni, Kandasamy Karthikeyan, Farhin R Khan, Jayaprakash Raghul, Govindharajan Vijaykanth, Shridhar Narayanan
Vorinostat [suberoylanilide hydroxamic acid (SAHA)], a histone deacetylase inhibitor, shows limited clinical activity against solid tumors when used alone. The methyl xanthine drug, pentoxifylline (PENT), has been described to have antitumor properties. The aim of this study was to look for the enhanced anticancer activities of both agents when used in combination at doses lower than their respective efficacy dose when used alone. We investigated the antitumor potential of this novel combination in vitro and in vivo...
July 19, 2017: Anti-cancer Drugs
https://www.readbyqxmd.com/read/28720062/hes1-overexpression-promotes-cell-proliferation-and-invasion-in-breast-cancer-and-predicts-poor-survival
#11
Xiaoying Li, Yu Cao, Mu Li, Feng Jin
HES1 is a transcriptional repressor involved in cell differentiation and proliferation, but the expression pattern and biological roles of HES1 in breast cancer have not been examined. In this study, we examined HES1 expression in breast cancer tissues using immunohistochemistry and western blot analyses and investigated HES1 function using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Matrigel invasion assays. Significant relationships were observed between HES1 upregulation and advanced tumour-node-metastasis stage (p = 0...
July 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28718964/antiangiogenic-effects-of-ziyuglycoside-ii-a-major-active-compound-of-sanguisorba-officinalis-l
#12
Sang-Hyeon Nam, Khaliunaa Lkhagvasuren, Hee Won Seo, Jin-Kyung Kim
Ziyuglycoside II, a major bioactive compound of Sanguisorba officinalis L., displays anticancer potential against several human cancer cells. However, little information concerning its antiangiogenic properties and possible mechanisms is available. The aim of this study was to investigate the inhibitory effects of ziyuglycoside II on angiogenesis. Ziyuglycoside II inhibited the proliferation, migration, and tubule formation of human umbilical vein endothelial cells, as well as the number of microvessels growing from the aortic rings...
July 18, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28717426/extracellular-vesicles-released-following-heat-stress-induce-bystander-effect-in-unstressed-populations
#13
Findlay Bewicke-Copley, Laura Ann Mulcahy, Laura Ann Jacobs, Priya Samuel, Naveed Akbar, Ryan Charles Pink, David Raul Francisco Carter
Cells naïve to stress can display the effects of stress, such as DNA damage and apoptosis, when they are exposed to signals from stressed cells; this phenomenon is known as the bystander effect. We previously showed that bystander effect induced by ionising radiation are mediated by extracellular vesicles (EVs). Bystander effect can also be induced by other types of stress, including heat shock, but it is unclear whether EVs are involved. Here we show that EVs released from heat shocked cells are also able to induce bystander damage in unstressed populations...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28717225/a-mammalian-mirtron-mir-1224-promotes-tube-formation-of-human-primary-endothelial-cells-by-targeting-anti-angiogenic-factor-epsin2
#14
Eiko Sakai, Yusuke Miura, Emi Suzuki-Kouyama, Kengo Oka, Masashi Tachibana, Kenji Kawabata, Fuminori Sakurai, Hiroyuki Mizuguchi
Angiogenesis, new vessel formation from pre-existing vessels, is a highly conserved event through vertebrates. However, the system for tuning angiogenesis by species-intrinsic factors is totally unknown. miR-1224 is a member of mammal-specific mirtrons, which were identified as non-canonical microRNAs. We found that the expression of miR-1224 was upregulated in capillary-like tube-forming human umbilical vein endothelial cells on Matrigel. Enforced expression of miR-1224 stimulated tube formation, whereas repression of endogenous miR-1224 inhibited formation...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28717185/clinical-significance-and-effect-of-lncrna-hoxa11-as-in-nsclc-a-study-based-on-bioinformatics-in-vitro-and-in-vivo-verification
#15
Yu Zhang, Wen-Jie Chen, Ting-Qing Gan, Xiu-Ling Zhang, Zu-Cheng Xie, Zhi-Hua Ye, Yun Deng, Ze-Feng Wang, Kai-Teng Cai, Shi-Kang Li, Dian-Zhong Luo, Gang Chen
HOXA11 antisense RNA (HOXA11-AS) has been shown to be involved in tumorigenesis and development of different cancers. However, the role of HOXA11-AS in non-small cell lung cancer (NSCLC) remains unclear. In this study, we firstly explored and confirmed the expression of HOXA11-AS in NSCLC tissues and cells. Cytometry, CCK-8, cell scratch, migration, Matrigel invasion and flow cytometry assays were performed to determine the biological impact of HOXA11-AS in vitro. Furthermore, a chick embryo chorioallantoic membrane (CAM) model of NSCLC was constructed to explore the effect of HOXA11-AS on tumorigenicity and angiogenesis in vivo...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28717129/3d-printed-scaffolds-of-calcium-silicate-doped-%C3%AE-tcp-synergize-with-co-cultured-endothelial-and-stromal-cells-to-promote-vascularization-and-bone-formation
#16
Yuan Deng, Chuan Jiang, Cuidi Li, Tao Li, Mingzheng Peng, Jinwu Wang, Kerong Dai
Synthetic bone scaffolds have potential application in repairing large bone defects, however, inefficient vascularization after implantation remains the major issue of graft failure. Herein, porous β-tricalcium phosphate (β-TCP) scaffolds with calcium silicate (CS) were 3D printed, and pre-seeded with co-cultured human umbilical cord vein endothelial cells (HUVECs) and human bone marrow stromal cells (hBMSCs) to construct tissue engineering scaffolds with accelerated vascularization and better bone formation...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28710377/antiangiogenic-activity-of-phthalides-enriched-angelica-sinensis-extract-by-suppressing-wsb-1-pvhl-hif-1%C3%AE-vegf-signaling-in-bladder-cancer
#17
Meng-Chuan Chen, Wen-Lin Hsu, Wen-Liang Chang, Tz-Chong Chou
The hypoxia-inducible factor-1α (HIF-1α) plays a critical role in tumor angiogenesis. It has been reported that the acetone extract of Angelica sinensis (AE-AS) rich in phthalides is able to inhibit cancer cell proliferation. However, whether AE-AS reduces cancer angiogenesis remains unknown. In this study, we demonstrated that AE-AS significantly inhibited the angiogenesis in vitro and in vivo evidenced by attenuation of the tube formation in hypoxic human umbilical vascular endothelial cells (HUVECs), and the vasculature generation in Matrigel plug, the chicken chorioallantoic membrane, and tumors...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28705238/interleukin-3-enhances-the-migration-of-human-mesenchymal-stem-cells-by-regulating-expression-of-cxcr4
#18
Amruta Barhanpurkar-Naik, Suhas T Mhaske, Satish T Pote, Kanupriya Singh, Mohan R Wani
BACKGROUND: Mesenchymal stem cells (MSCs) represent an important source for cell therapy in regenerative medicine. MSCs have shown promising results for repair of damaged tissues in various degenerative diseases in animal models and also in human clinical trials. However, little is known about the factors that could enhance the migration and tissue-specific engraftment of exogenously infused MSCs for successful regenerative cell therapy. Previously, we have reported that interleukin-3 (IL-3) prevents bone and cartilage damage in animal models of rheumatoid arthritis and osteoarthritis...
July 14, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28701863/inhibition-of-hypoxia-induced-cyclooxygenase-2-by-korean-red-ginseng-is-dependent-on-peroxisome-proliferator-activated-receptor%C3%A2-gamma
#19
Heewon Song, Young Joo Lee
BACKGROUND: Korean Red Ginseng (KRG) is a traditional herbal medicine made by steaming and drying fresh ginseng. It strengthens the endocrine and immune systems to ameliorate various inflammatory responses. The cyclooxygenase-2 (COX-2)/prostaglandin E2 pathway has important implications for inflammation responses and tumorigenesis. Peroxisome proliferator-activated receptor gamma (PPARγ) is a transcription factor that regulates not only adipogenesis and lipid homeostasis, but also angiogenesis and inflammatory responses...
July 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28699179/colorectal-cancer-stem-cells-acquire-chemoresistance-through-the-upregulation-of-fbxw7-and-the-consequent-degradation-of-c-myc
#20
Daisuke Izumi, Takatsugu Ishimoto, Keisuke Miyake, Tsugio Eto, Kota Arima, Yuki Kiyozumi, Tomoyuki Uchihara, Junji Kurashige, Masaaki Iwatsuki, Yoshifumi Baba, Yasuo Sakamoto, Yuji Miyamoto, Naoya Yoshida, Masayuki Watanabe, Ajay Goel, Patrick Tan, Hideo Baba
The cancer stem cell (CSC) paradigm suggests that tumors are organized hierarchically. We previously established an LGR5(+) human colorectal cancer stem-cell-enriched cell line (colorectal CSCs) that expresses well-accepted colorectal CSC markers and that can dynamically switch between proliferative and drug-resistant non-cycling states. We performed this study to elucidate the molecular mechanisms responsible for evading cell death in colorectal CSCs mediated by anticancer agents. During the cell cycle arrest caused by anticancer agents, we found that c-Myc expression was substantially decreased in colorectal CSCs...
July 11, 2017: Stem Cells
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