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https://www.readbyqxmd.com/read/28524833/promoting-vasa-vasorum-neovascularization-of-vein-grafts-extenuates-hypoxia-of-the-wall-and-its-subsequent-influence-on-intimal-hyperplasia
#1
Rong-Jiang Zou, Zheng-Hua Wang, Chen-Xi Wang, Song Xue
BACKGROUND: The autologous saphenous vein is the most common conduit for coronary artery bypass grafting, but the vein graft disease will occur. This study used Matrigel basement membrane matrix with many different growth factors to promote vasa vasorum neovascularization and extenuate the hypoxia to improve remodeling. METHODS: This study observed the hypoxia and thickness of the vein grafts at different times. Normal veins and vein grafts with 15 min of ischemia one day postoperatively were harvested in the neck of rabbits...
June 5, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28524367/transplantation-of-human-villous-trophoblasts-preserves-cardiac-function-in-mice-with-acute-myocardial-infarction
#2
Zegen Wang, Ningzheng Dong, Yayan Niu, Zhiwei Zhang, Ce Zhang, Meng Liu, Tiantian Zhou, Qingyu Wu, Ke Cheng
Over the past decade, cell therapies have provided promising strategies for the treatment of ischaemic cardiomyopathy. Particularly, the beneficial effects of stem cells, including bone marrow stem cells (BMSCs), endothelial progenitor cells (EPCs), mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), and induced pluripotent stem cells (iPSCs), have been demonstrated by substantial preclinical and clinical studies. Nevertheless stem cell therapy is not always safe and effective. Hence, there is an urgent need for alternative sources of cells to promote cardiac regeneration...
May 19, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28522946/parthenolide-promotes-apoptotic-cell-death-and-inhibits-the-migration-and-invasion-of-sw620-cells
#3
Yu Chuan Liu, Se Lim Kim, Young Ran Park, Soo-Teik Lee, Sang Wook Kim
BACKGROUND/AIMS: Parthenolide (PT), a principle component derived from feverfew (Tanacetum parthenium), is a promising anticancer agent and has been shown to promote apoptotic cell death in various cancer cells. In this study, we focused on its functional role in apoptosis, migration, and invasion of human colorectal cancer (CRC) cells. METHODS: SW620 cells were employed as representative human CRC cells. We performed the MTT assay and cell cycle analysis to measure apoptotic cell death...
April 2017: Intestinal Research
https://www.readbyqxmd.com/read/28521261/novel-anti-inflammatory-agents-targeting-cxcr4-design-synthesis-biological-evaluation-and-preliminary-pharmacokinetic-study
#4
Renren Bai, Zhongxing Liang, Younghyoun Yoon, Eric Salgado, Amber Feng, Saumya Gurbani, Hyunsuk Shim
CXCR4 plays a crucial role in the inflammatory disease process, providing an attractive means for drug targeting. A series of novel amide-sulfamide derivatives were designed, synthesized and comprehensively evaluated. This new scaffold exhibited much more potent CXCR4 inhibitory activity, with more than 70% of the compounds showed notably better binding affinity than the reference drug AMD3100 in the binding assay. Additionally, in the Matrigel invasion assay, most of our compounds significantly blocked the tumor cell invasion, demonstrating superior efficacy compared to AMD3100...
May 10, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28516350/evaluation-of-vascular-effect-of-arsenic-using-in-vivo-assays
#5
Bharat Patel, Rajat Das, Anil Gautam, Mugdha Tiwari, Sukhdev Acharya, Sunil Kumar
Arsenic (As) is an abundant toxicant present in groundwater and soil in various parts of the world including eastern part of India. The epidemiological studies have shown that arsenic exposure is linked to developmental defects and miscarriage. Placenta is known to utilize vasculogenesis to develop its vasculature circulation. The effects of four different doses of sodium meta-arsenite (0, 10, 20, 75, and 150 ppm) were assessed on the vascular structure using two different in vivo models, i.e., Matrigel and chorioallantoic membrane (CAM) assay...
May 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28516164/engineered-ligand-based-vegfr-antagonists-with-increased-receptor-binding-affinity-more-effectively-inhibit-angiogenesis
#6
Shiven Kapur, Adam P Silverman, Anne Z Ye, Niv Papo, Darren Jindal, Mark S Blumenkranz, Jennifer R Cochran
Pathologic angiogenesis is mediated by the coordinated action of the vascular endothelial growth factor (VEGF)/vascular endothelial growth factor receptor 2 (VEGFR2) signaling axis, along with crosstalk contributed by other receptors, notably αvβ3 integrin. We build on earlier work demonstrating that point mutations can be introduced into the homodimeric VEGF ligand to convert it into an antagonist through disruption of binding to one copy of VEGFR2. This inhibitor has limited potency, however, due to loss of avidity effects from bivalent VEGFR2 binding...
March 2017: Bioengineering & Translational Medicine
https://www.readbyqxmd.com/read/28512172/in-vivo-hemin-conditioning-targets-the-vascular-and-immunological-compartments-and-restrains-prostate-tumor-development
#7
Felipe M Jaworski, Lucas Gentilini, Geraldine Gueron, Roberto P Meiss, Emiliano G Ortiz, Paula M Berguer, Asif Ahmed, Nora M Navone, Gabriel A Rabinovich, Daniel Compagno, Diego J Laderach, Elba S Vazquez
Purpose: Conditioning strategies constitute a relatively unexplored and exciting opportunity to shape tumor fate by targeting the tumor microenvironment. In this study we assessed how hemin, a pharmacological inducer of Heme Oxygenase-1 (HO-1), impacts upon prostate cancer (PCa) development in an in vivo conditioning model. <p>Experimental Design: The stroma of C57BL/6 mice was conditioned by subcutaneous administration of hemin prior to TRAMP-C1 tumor challenge. Complementary in vitro and in vivo assays were performed to evaluate hemin effect on both angiogenesis and the immune response...
May 16, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28510488/distinct-effects-of-growth-hormone-and-glutamine-on-activation-of-intestinal-stem-cells
#8
Yun Chen, Sheng-Hong Tseng, Chao-Ling Yao, Chuan Li, Ya-Hui Tsai
BACKGROUND: For patients with short bowel syndrome under parenteral nutrition support, growth hormone (GH) and glutamine (GLN) have been found to help the growth of intestinal mucosa. In this research, we studied the effects of GH and GLN on intestinal stem cells (ISCs). METHODS: The in vitro and in vivo effects of GH and/or GLN on ISCs were evaluated by observing the ability of ISCs to form organoids in a Matrigel culture system. The expression levels of stemness and differentiation markers in ISCs and organoids were assessed using quantitative real-time polymerase chain reaction, immunofluorescence assay, and immunohistochemistry staining...
May 1, 2017: JPEN. Journal of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28507177/evidence-for-a-derangement-of-the-microvascular-system-in-patients-with-a-very-early-diagnosis-of-systemic-sclerosis
#9
Inês Chora, Eloisa Romano, Mirko Manetti, Celestina Mazzotta, Raquel Costa, Vera Machado, Alice Cortez, Cosimo Bruni, Gemma Lepri, Serena Guiducci, Amato De Paulis, Raquel Soares, Marco Matucci-Cerinic
OBJECTIVE: To investigate whether patients with a very early diagnosis of systemic sclerosis (VEDOSS) may already present circulating markers and in vitro signs of microvascular dysfunction. METHODS: Serum samples were obtained from 55 patients with systemic sclerosis (SSc), 25 patients with VEDOSS, and 55 matched healthy controls (HC). Serum levels of pan-vascular endothelial growth factor (VEGF) and soluble neuropilin-1 (sNRP-1) were measured by ELISA. Human dermal microvascular endothelial cells (H-MVEC) were cultured and stimulated with SSc, VEDOSS, and HC sera...
May 15, 2017: Journal of Rheumatology
https://www.readbyqxmd.com/read/28499803/establishment-and-characterization-of-primary-astrocyte-culture-from-adult-mouse-brain
#10
Xiu Sun, Xin Hu, Dan Wang, Yimin Yuan, Shangyao Qin, Zijian Tan, Yakun Gu, Xiao Huang, Cheng He, Zhida Su
As a major class of glial cells, astrocytes have been indicated to play multi-roles in physiological and pathological brain. Astrocyte cultures derived from postnatal mouse brains have been extensively used to characterize their biological properties. However, the inability to culture adult mouse primary astrocytes has long stymied studies of function in adult brain. Here, we developed a protocol to successfully establish highly enriched astrocyte cultures from the brains of adult mouse. Cortical tissues were collected to prepare cell suspension by enzymatic digestion and mechanical dissociation, and then plated onto vessels pre-coated with gelatin and matrigel and cultured in DMEM medium containing 20% fetal bovine serum (FBS)...
May 10, 2017: Brain Research Bulletin
https://www.readbyqxmd.com/read/28494923/effects-of-serum-amyloid-a-on-matrix-metalloproteinase-9-production-in-feline-lymphoma-derived-cell-lines
#11
Takashi Tamamoto, Koichi Ohno, Yuko Goto-Koshino, Hajime Tsujimoto
Serum amyloid A (SAA) concentration and plasma matrix metalloproteinase-9 (MMP-9) levels are increased in cats with lymphoma. In the present study, the association between SAA and MMP-9 production was evaluated using recombinant feline SAA (rfSAA) and three feline lymphoma-derived cell lines: 3201, MS4, and MCC. MMP-9 mRNA expression was significantly increased by rfSAA stimulation only in MCC cells. Secreted MMP-9 protein in culture media was confirmed by gelatin zymography, with clear bands of MMP-9 detected in MCC cells following rfSAA stimulation...
May 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28488588/leaf-inspired-microcontact-printing-vascular-patterns
#12
Lian Wong, John Pegan, Basia Gabela-Zuniga, Michelle Khine, Kara E McCloskey
The vascularization of tissue grafts is critical for maintaining viability of the cells within a transplanted graft. A number of strategies are currently being investigated including very promising microfluidics systems. Here, we explored the potential for generating a vasculature-patterned endothelial cells (EC) that could be integrated into distinct layers between sheets of primary cells. Bioinspired from the leaf veins, we generated a reverse mold with a fractal vascular-branching pattern that models the unique spatial arrangement over multiple length scales that precisely mimic branching vasculature...
May 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28486399/assessment-of-heparanase-mediated-angiogenesis-using-microvascular-endothelial-cells-identification-of-%C3%AE-carrageenan-derivative-as-a-potent-anti-angiogenic-agent
#13
Nicolas Poupard, Pamela Badarou, Fabienne Fasani, Hugo Groult, Nicolas Bridiau, Frédéric Sannier, Stéphanie Bordenave-Juchereau, Claudine Kieda, Jean-Marie Piot, Catherine Grillon, Ingrid Fruitier-Arnaudin, Thierry Maugard
Heparanase is overexpressed by tumor cells and degrades the extracellular matrix proteoglycans through cleavage of heparan sulfates (HS), allowing pro-angiogenic factor release and thus playing a key role in tumor angiogenesis and metastasis. Here we propose new HS analogs as potent heparanase inhibitors: Heparin as a positive control, Dextran Sulfate, λ-Carrageenan, and modified forms of them obtained by depolymerization associated to glycol splitting (RD-GS). After heparanase activity assessment, 11 kDa RD-GS-λ-Carrageenan emerged as the most effective heparanase inhibitor with an IC50 of 7...
May 9, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28484743/effects-of-cell-cycle-regulators-on-the-cell-cycle-synchronization-of-porcine-induced-pluripotent-stem-cells
#14
Dae-Jin Kwon, In-Sul Hwang, Tae-Uk Kwak, Hyeon Yang, Mi-Ryung Park, Sun-A Ock, Keon Bong Oh, Jae-Seok Woo, Gi-Sun Im, Seongsoo Hwang
Unlike mouse results, cloning efficiency of nuclear transfer from porcine induced pluripotent stem cells (piPSCs) is very low. The present study was performed to investigate the effect of cell cycle inhibitors on the cell cycle synchronization of piPSCs. piPSCs were generated using combination of six human transcriptional factors under stem cell culture condition. To examine the efficiency of cell cycle synchronization, piPSCs were cultured on a matrigel coated plate with stem cell media and they were treated with staurosporine (STA, 20 nM), daidzein (DAI, 100 μM), roscovitine (ROSC, 10 μM), or olomoucine (OLO, 200 μM) for 12 h...
March 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28484097/fabrication-of-a-matrigel-collagen-semi-interpenetrating-scaffold-for-use-in-dynamic-valve-interstitial-cell-culture
#15
Ngoc Lam, Hanh Lam, Nasya Sturdivant, Kartik Balachandran
The study of heart valve homeostatic and disease mechanisms are often limited by the challenges in simulating the in vivo milieu, where valve cells are surrounded by the extracellular matrix in a three dimensional environment and experience multiple dynamic mechanical forces. Type I collagen is typically the most common three dimensional matrix used to culture valve cells in vitro. Unfortunately, this material has poor mechanical behavior due to an inherent propensity to compact significantly, unlike native valve leaflets...
May 9, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28483438/construction-of-vascularized-pacemaker-tissues-by-seeding-cardiac-progenitor-cells-and-endothelial-progenitor-cells-into-matrigel
#16
Luping Zhang, Xiaotong Li, Xiaolong Yu, Yuquan Li, Aijun Sun, Chao Huang, Feng Xu, Jinping Guo, Yao Sun, Xi Zhang, Xiangqun Yang, Chuansen Zhang
AIMS: Transplantation of a tissue engineered cardiac pacemaker (TECP) may represent a novel therapy for cardiac sinus node dysfunction. We previously reported that cardiac progenitor cells (CPCs) derived from embryonic heart tubes could differentiate into cardiac pacemaking cells after endothelin-1 treatment. We aimed to examine the feasibility of TECP fabricated from CPCs-derived pacemaking cells and vascularization of TECP fabricated from CPCs-derived pacemaking cells and endothelial progenitor cells (EPCs) in vitro and in vivo implantation...
May 5, 2017: Life Sciences
https://www.readbyqxmd.com/read/28479957/4-hydroxynonenal-contributes-to-angiogenesis-through-a-redox-dependent-sphingolipid-pathway-prevention-by-hydralazine-derivatives
#17
Caroline Camaré, Corinne Vanucci-Bacqué, Nathalie Augé, Mélanie Pucelle, Corinne Bernis, Audrey Swiader, Michel Baltas, Florence Bedos-Belval, Robert Salvayre, Anne Nègre-Salvayre
The neovascularization of atherosclerotic lesions is involved in plaque development and may contribute to intraplaque hemorrhage and plaque fragilization and rupture. Among the various proangiogenic agents involved in the neovascularization process, proatherogenic oxidized LDLs (oxLDLs) contribute to the formation of tubes via the generation of sphingosine 1-phosphate (S1P), a major mitogenic and proangiogenic sphingolipid mediator. In this study, we investigated whether 4-hydroxynonenal (4-HNE), an aldehydic lipid oxidation product abundantly present in oxLDLs, contributes to their proangiogenic properties...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28473446/human-plasma-thioredoxin-80-increases-with-age-and-in-apoe-mice-induces-inflammation-angiogenesis-and-atherosclerosis
#18
Dominique Couchie, Boris Vaisman, Amna Abderrazak, Dler Faieeq Darweesh Mahmood, Magda M Hamza, Fanny Canesi, Vimala Diderot, Khadija El Hadri, Anne E Nègre-Salvayre, Aurélie Le Page, Tamas Fulop, Alan T Remaley, Mustapha Rouis
Background -Thioredoxin-1 (TRX1), a ubiquitous 12 kDa protein, exerts anti-oxidant and anti-inflammatory effects. In contrast, the truncated form, called TRX80, produced by macrophages induces up-regulation of pro-inflammatory cytokines. TRX80 also promotes the differentiation of mouse peritoneal and human macrophages toward a pro-inflammatory M1 phenotype. Methods -TRX1 and TRX80 plasma levels were determined using specific ELISA. ADAM-17 and ADAM-10 activities were measured using respectively SensoLyte® 520 ADAM10 Activity Assay Kit *Fluorimetric* and InnoZyme™ TACE Activity Kit...
May 4, 2017: Circulation
https://www.readbyqxmd.com/read/28472059/reactive-oxygen-species-modulator-1-romo1-predicts-unfavorable-prognosis-in-colorectal-cancer-patients
#19
Hong Jun Kim, Min Jee Jo, Bo Ram Kim, Jung Lim Kim, Yoon A Jeong, Yoo Jin Na, Seong Hye Park, Suk-Young Lee, Dae-Hee Lee, Hye Seung Lee, Baek-Hui Kim, Sun Il Lee, Byung Wook Min, Young Do Yoo, Sang Cheul Oh
BACKGROUND: Reactive oxygen species modulator-1 (Romo1) is a novel protein that has been reported to be crucial for cancer cell proliferation and invasion. However, its clinical implications in colorectal cancer patients are not well-known. For the first time, we investigated the association between Romo1 and the clinical outcomes of colorectal cancer patients. STUDY: We examined Romo1 expression in resected tumor tissues immunohistochemically and assessed it with histological scores...
2017: PloS One
https://www.readbyqxmd.com/read/28468945/primary-cilia-control-the-maturation-of-tubular-lumen-in-renal-collecting-duct-epithelium
#20
Thomas Ernandez, Olga Komarynets, Alexandra Chassot, Soushma Sougoumarin, Priscilla Soulie, Yubao Wang, Roberto Montesano, Eric Feraille
The key role of the primary cilium in developmental processes is illustrated by ciliopathies resulting from genetic defects of its components. Ciliopathies include a large variety of dysmorphic syndromes that share in common the presence of multiple kidney cysts. These observations suggest that primary cilia may control morphogenetic processes in the developing kidney. In this study, we assessed the role of primary cilium in branching tubulogenesis and/or lumen development using kidney collecting duct-derived mCCDN21 cells that display spontaneous tubulogenic properties when grown in collagen/matrigel matrix...
May 3, 2017: American Journal of Physiology. Cell Physiology
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