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https://www.readbyqxmd.com/read/29344334/collective-adhesion-and-displacement-of-retinal-progenitor-cells-upon-extracellular-matrix-substrates-of-transplantable-biomaterials
#1
Ankush Thakur, Shawn Mishra, Juan Pena, Jing Zhou, Stephen Redenti, Robert Majeska, Maribel Vazquez
Strategies to replace retinal photoreceptors lost to damage or disease rely upon the migration of replacement cells transplanted into sub-retinal spaces. A significant obstacle to the advancement of cell transplantation for retinal repair is the limited migration of transplanted cells into host retina. In this work, we examine the adhesion and displacement responses of retinal progenitor cells on extracellular matrix substrates found in retina as well as widely used in the design and preparation of transplantable scaffolds...
January 2018: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29344204/silibinin-inhibits-the-migration-and-invasion-of-human-gastric-cancer-sgc7901-cells-by-downregulating-mmp-2-and-mmp-9-expression-via-the-p38mapk-signaling-pathway
#2
Shuming Lu, Zhuqing Zhang, Meiru Chen, Chunyan Li, Lina Liu, Yan Li
The objective of the present study was to observe the effects of silibinin and the p38 mitogen-activated protein kinase (MAPK) signaling pathway inhibitor SB203580 on the migration and invasion capabilities of SGC7901 cells, and to explore the underlying associated mechanisms. Scratch, Transwell and Matrigel invasion assays were performed to study the effects of silibinin on cell migration and invasion. Western blot analysis was used to determine the expression levels of p38MAPK, phosphorylated (p-)p38MAPK, matrix metalloproteinase (MMP)-2 and MMP-9...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29344126/microrna-34a-overexpression-inhibits-cell-migration-and-invasion-via-regulating-sirt1-in-hepatocellular-carcinoma
#3
Jianhui Zhou, Wenying Zhou, Fangen Kong, Xiaoyu Xiao, Haoyu Kuang, Yingxian Zhu
Hepatocellular carcinoma (HCC) remains one of the most common types of malignancy with high mortality and morbidity rates. Previous studies have suggested that microRNAs (miRs) serve pivotal functions in various types of tumor. The aim of the present study was to assess the association between miR-34a expression and HCC cell migration and invasion, and the potential underlying mechanisms. The miR-34a overexpression vector or scramble control was transfected into human Hep3B and Huh7 cell lines. Transwell assays, and Matrigel and wound healing assays were used to detect the effects of miR-34a expression on HCC cell invasion and migration, respectively...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29344123/ex-vivo-model-of-non-small-cell-lung-cancer-using-mouse-lung-epithelial-cells
#4
Taku Sato, Mami Morita, Ryota Tanaka, Yui Inoue, Miyuki Nomura, Yoshimi Sakamoto, Koh Miura, Shigemi Ito, Ikuro Sato, Nobuyuki Tanaka, Jiro Abe, Satomi Takahashi, Masaaki Kawai, Masami Sato, Yoshitaka Hippo, Hiroshi Shima, Yoshinori Okada, Nobuhiro Tanuma
Lung cancer is the most common cause of cancer mortality, however, efficient methods to culture, expand and transform lung epithelial (LE) cells have not been established. In the present study, an efficient ex vivo method was applied to recapitulate lung carcinogenesis using mouse LE cells. A Matrigel-assisted three-dimensional culture was used to isolate and selectively expand LE cells from mouse lungs. Purified LE cells were passaged and expanded for at least 2 to 3 months while maintaining epidermal growth factor-dependence...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29339729/ecotropic-viral-integration-site-1-promotes-metastasis-independent-of-epithelial-mesenchymal-transition-in-colon-cancer-cells
#5
Kasturi Bala Nayak, I S Sajitha, T R Santhosh Kumar, Soumen Chakraborty
The most indecipherable component of solid cancer is the development of metastasis which accounts for more than 90% of cancer-related mortalities. A developmental program termed epithelial-mesenchymal transition (EMT) has also been shown to play a critical role in promoting metastasis in epithelium-derived solid tumors. By analyzing publicly available microarray datasets, we observed that ecotropic viral integration site 1 (EVI1) correlates negatively with SLUG, a master regulator of EMT. This correlation was found to be relevant as we demonstrated that EVI1 binds to SLUG promoter element directly through the distal set of zinc fingers and downregulates its expression...
January 16, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29330502/targeting-insulin-like-growth-factor-i-and-extracellular-matrix-interactions-in-melanoma-progression
#6
Berline Murekatete, Ali Shokoohmand, Jacqui McGovern, Lipsa Mohanty, Christoph Meinert, Brett G Hollier, Alfred Zippelius, Zee Upton, Abhishek S Kashyap
Insulin-like growth factor (IGF)-I binds to the ECM protein vitronectin (VN) through IGF binding proteins (IGFBPs) to enhance proliferation and migration of skin keratinocytes and fibroblasts. Although evidence exists for the role of individual components of the complex (IGF-I, IGFBP-3 and VN), the cellular functions stimulated by these proteins together as a complex remains un-investigated in melanoma cells. We report here that the IGF-I:IGFBP-3:VN trimeric complex stimulates a dose-dependent increase in the proliferation and migration of WM35 and Sk-MEL28 melanoma cells...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29329397/minar1-is-a-notch2-binding-protein-that-inhibits-angiogenesis-and-breast-cancer-growth
#7
Rachel Xi-Yeen Ho, Rosana D Meyer, Kevin B Chandler, Esma Ersoy, Michael Park, Philip A Bondzie, Nima Rahimi, Huihong Xu, Catherine E Costello, Nader Rahimi
Intrinsically disordered proteins (IDPs)/intrinsically unstructured proteins (IUPs) are characterized by the lack of fixed or stable tertiary structure, and are increasingly recognized as an important class of proteins with major roles in signal transduction and transcriptional regulation. In this study, we report the identification and functional characterization of a previously uncharacterized protein (UPF0258/KIAA1024), major intrinsically disordered Notch2-associated receptor 1 (MINAR1). While MINAR1 carries a single transmembrane domain and a short cytoplasmic domain, it has a large extracellular domain that shares no similarity with known protein sequences...
January 10, 2018: Journal of Molecular Cell Biology
https://www.readbyqxmd.com/read/29328476/effect-of-a-synthetic-inhibitor-of-urokinase-plasminogen-activator-on-the-migration-and-invasion-of-human-cervical-cancer-cells-in-vitro
#8
Xuechun Wang, Zhongqing Jiang, Jian An, Xiaodan Mao, Fen Lin, Pengming Sun
As a notable feature of malignant tumors, invasion and metastasis are important events in the process of tumor progression. Amiloride, a synthetic inhibitor of urokinase plasminogen activator (uPA), is involved in these events. To evaluate the therapeutic value of amiloride in cervical cancer, HeLa cells were used as in vitro cellular models. The migration and invasion abilities of HeLa cells, in addition to the mRNA expression of matriptase, uPA, uPA receptor and 72 kDa type IV collagenase (MMP‑2), were detected using scratch assays, Transwell chamber assays and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR)...
January 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29328471/prostaglandin-e2-receptor-ep4-is-involved-in-the-cell-growth-and-invasion-of-prostate-cancer-via-the-camp%C3%A2-pka-pi3k%C3%A2-akt-signaling-pathway
#9
Song Xu, Wenquan Zhou, Jingping Ge, Zhengyu Zhang
Prostate cancer (PCa) is one of the most prevalent diagnosed malignancies globally. Previous studies have demonstrated that prostaglandin E2 (PGE2) is closely associated with the tumorigenesis and progression of PCa. However, the underlying molecular mechanisms remain unclear and require further investigation. Matrix metalloproteinases (MMPs), receptor activator of nuclear factor‑κB ligand (RANKL) and runt‑related transcription factor 2 (RUNX2), which are involved in cell growth and bone metastasis, are frequently activated or overexpressed in various types of cancer, including PCa...
January 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29328455/role-of-mir-21-in-the-growth-and-metastasis-of-human-salivary-adenoid-cystic-carcinoma
#10
Fei Yan, Chao Wang, Ting Li, Wenyan Cai, Jinhu Sun
Aberrant microRNA (miRNA/miR) expression has been reported in various cancer types. miR‑21, which is considered to be a proto-oncogene and is frequently overexpressed in certain cancer types, has been implicated in tumorigenesis. The aim of the present study was to investigate the effect of miR‑21 degradation on tumor progression and its potential mechanisms in human salivary adenoid cystic carcinoma (SACC) development. Results of reverse transcription‑quantitative polymerase chain reaction analysis indicated that SACC cells with high metastatic potential (SACC‑LM cells) exhibited a significantly higher expression of miR‑21 compared with SACC cells with a lower metastatic potential (SACC‑83 cells)...
January 5, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29328441/ubenimex-an-apn-inhibitor-could-serve-as-an-anti%C3%A2-tumor-drug-in-rt112-and-5637-cells-by-operating-in-an-akt%C3%A2-associated-manner
#11
Xiaoqing Wang, Yang Liu, Wei Liu, Yongfei Zhang, Feng Guo, Lijuan Zhang, Mingyu Cui, Shuai Liu, Rongde Wu
Bladder cancer, a common urinary tract tumor, has high mortality and recurrence rates associated with metastasis. Aminopeptidase N (APN) expression and metastasis have been indicated to be associated with one another. Ubenimex may function as an APN inhibitor to inhibit the degradation of the extracellular matrix during tumorigenesis. Furthermore, APN has been widely used as an adjuvant therapy for the treatment of tumors; however, little information is available regarding the impact of ubenimex on patients...
January 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29316827/downregulation-of-mage-family-member-h1-enhances-hepatocellular-carcinoma-progression-and-serves-as-a-biomarker-for-patient-prognosis
#12
Peng-Cheng Wang, Zhi-Qiang Hu, Shao-Lai Zhou, Hao Zhan, Zheng-Jun Zhou, Chu-Bin Luo, Xiao-Wu Huang
AIM: The MAGE family member H1 (MAGEH1) belongs to melanoma-associated antigen (MAGE) superfamily. The role of MAGEH1 in hepatocellular carcinoma (HCC) is largely undefined. MATERIALS & METHODS: We used quantitative reverse transcription PCR and immunohistochemistry to detect MAGEH1 expression in HCC tissues. CCK-8 assay, wound healing migration assay and Transwell Matrigel invasion assay were used to measure HCC cell proliferation, migration and invasion ability...
January 10, 2018: Future Oncology
https://www.readbyqxmd.com/read/29312602/tanshinone-iia-inhibits-angiogenesis-in-human-endothelial-progenitor-cells-in-vitro-and-in-vivo
#13
Hsiang-Ping Lee, Yueh-Ching Liu, Po-Chun Chen, Huai-Ching Tai, Te-Mao Li, Yi-Chin Fong, Chih-Shiang Chang, Min-Huan Wu, Li-Pin Chiu, Chia-Jung Wang, Yi-Hsuan Chen, Yih-Jer Wu, Chih-Hsin Tang, Shih-Wei Wang
Accumulating evidence reports that bone marrow-derived endothelial progenitor cells (EPCs) regulate angiogenesis, postnatal neovascularization and tumor metastasis. It has been suggested that understanding the molecular targets and pharmacological functions of natural products is important for novel drug discovery. Tanshinone IIA is a major diterpene quinone compound isolated from Danshen (Salvia miltiorrhiza) and is widely used in traditional Chinese medicine (TCM). Evidence indicates that tanshinone IIA modulates angiogenic functions in human umbilical vein endothelial cells...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29304770/doxorubicin-resistance-in-breast-cancer-cells-is-mediated-by-extracellular-matrix-proteins
#14
Carrie J Lovitt, Todd B Shelper, Vicky M Avery
BACKGROUND: Cancer cell resistance to therapeutics can result from acquired or de novo-mediated factors. Here, we have utilised advanced breast cancer cell culture models to elucidate de novo doxorubicin resistance mechanisms. METHODS: The response of breast cancer cell lines (MCF-7 and MDA-MB-231) to doxorubicin was examined in an in vitro three-dimensional (3D) cell culture model. Cells were cultured with Matrigel™ enabling cellular arrangements into a 3D architecture in conjunction with cell-to-extracellular matrix (ECM) contact...
January 6, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29304536/defibrotide-stimulates-angiogenesis-and-protects-endothelial-cells-from-calcineurin-inhibitor-induced-apoptosis-via-upregulation-of-akt-bcl-xl
#15
Xiangmin Wang, Bin Pan, Yuko Hashimoto, Hiroshi Ohkawara, Kailin Xu, Lingyu Zeng, Takayuki Ikezoe
Sinusoidal obstruction syndrome is a life-threatening complication that can occur after haematopoietic stem cell transplantation. Defibrotide (DF) has been approved for the treatment of individuals with severe sinusoidal obstruction syndrome following haematopoietic stem cell transplantation in the European Union and the United States. However, the precise mechanisms by which DF protects endothelial cells remain to be elucidated. In this study, we found that DF stimulated angiogenesis in vitro and in vivo as assessed by vascular tube formation, scratch-wound repair and Matrigel plug assays...
January 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29300391/use-of-a-three-layer-gradient-system-of-cells-for-rat-testicular-organoid-generation
#16
João Pedro Alves-Lopes, Olle Söder, Jan-Bernd Stukenborg
We have recently developed a 3D culture system that allows the reorganization of rat primary testicular cells into organoids with a functional blood-testis barrier, as well as the establishment and maintenance of germ cells. The innovative aspect of our model, the three-layer gradient system (3-LGS), comprises cells combined with Matrigel placed between two layers of Matrigel without cells, which creates a gradient of cells and allows the reorganization of testicular cells into organized structures after 5-7 d in culture...
February 2018: Nature Protocols
https://www.readbyqxmd.com/read/29299781/slit2-robo1-signaling-is-involved-in-angiogenesis-of-glomerular-endothelial-cells-exposed-to-a-diabetic-like-environment
#17
Junhui Liu, Weiping Hou, Tao Guan, Luyao Tang, Xufei Zhu, Yi Li, Shihui Hou, Jun Zhang, Hua Chen, Yunjian Huang
Abnormal angiogenesis plays a pathological role in diabetic nephropathy (DN), contributing to glomerular hypertrophy and microalbuminuria. Slit2/Robo1 signaling participates in angiogenesis in some pathological contexts, but whether it is involved in glomerular abnormal angiogenesis of early DN is unclear. The present study evaluated the effects of Slit2/Robo1 signaling pathway on angiogenesis of human renal glomerular endothelial cells (HRGECs) exposed to a diabetic-like environment or recombinant Slit2-N...
January 3, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29299145/inhibition-of-yap-function-overcomes-braf-inhibitor-resistance-in-melanoma-cancer-stem-cells
#18
Matthew L Fisher, Daniel Grun, Gautam Adhikary, Wen Xu, Richard L Eckert
Treating BRAF inhibitor-resistant melanoma is an important therapeutic goal. Thus, it is important to identify and target mechanisms of resistance to improve therapy. The YAP1 and TAZ proteins of the Hippo signaling pathway are important drivers of cancer cell survival, and are BRAF inhibitor resistant factors in melanoma. We examine the role of YAP1/TAZ in melanoma cancer stem cells (MCS cells). We demonstrate that YAP1, TAZ and TEAD (TEA domain transcription factor) levels are elevated in BRAF inhibitor resistant MCS cells and enhance cell survival, spheroid formation, matrigel invasion and tumor formation...
December 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/29299143/structural-determinant-of-bst-2-mediated-regulation-of-breast-cancer-cell-motility-a-role-for-cytoplasmic-tail-tyrosine-residues
#19
Wasifa Naushad, Wadie D Mahauad-Fernandez, Chioma M Okeoma
There is now irrefutable evidence that overexpression of the innate immunity protein-BST-2, in breast cancer cells is implicated in tumor growth and progression. The cellular mechanisms that control BST-2-mediated effect in tumor progression involve enhancement of cancer cell motility-migration/invasion. However, the distinct structural elements of BST-2 that mediate breast cancer cell motility remain unknown. Here, we used various motility assays and different variants of BST-2 to examine the cellular and structural mechanisms controlling BST-2-mediated cell motility...
December 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/29296725/replenishing-exosomes-from-older-bone-marrow-stromal-cells-with-mir-340-inhibits-myeloma-related-angiogenesis
#20
Tomohiro Umezu, Satoshi Imanishi, Kenko Azuma, Chiaki Kobayashi, Seiichiro Yoshizawa, Kazuma Ohyashiki, Junko H Ohyashiki
The study of bone marrow stromal cells (BMSCs) and the exosomes they secrete is considered promising for cancer therapy. However, little is known about the effect of donor age on BMSCs. In the present study, we investigated the therapeutic potential of BMSC exosomes derived from donors of different ages using an in vivo model of hypoxic bone marrow in multiple myeloma (MM). We found that donor age was strongly related to senescent changes in BMSCs. Exosomes derived from young BMSCs significantly inhibited MM-induced angiogenesis in Matrigel plugs...
May 23, 2017: Blood Advances
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