keyword
MENU ▼
Read by QxMD icon Read
search

Robert Langer

keyword
https://www.readbyqxmd.com/read/29152410/ultrasound-triggered-local-anaesthesia
#1
Alina Y Rwei, Juan L Paris, Bruce Wang, Weiping Wang, Christopher D Axon, María Vallet-Regí, Robert Langer, Daniel S Kohane
On-demand relief of local pain would allow patients to control the timing, intensity and duration of nerve block in a safe and non-invasive manner. Ultrasound would be a suitable trigger for such a system, as it is in common clinical use and can penetrate deeply into the body. Here, we demonstrate that ultrasound-triggered delivery of an anaesthetic from liposomes allows the timing, intensity and duration of nerve block to be controlled by ultrasound parameters. On insonation, the encapsulated sonosensitizer protoporphyrin IX produces reactive oxygen species that react with the liposomal membrane, leading to the release of the potent local anaesthetic tetrodotoxin...
2017: Nature biomedical engineering
https://www.readbyqxmd.com/read/29131148/structure-guided-chemical-modification-of-guide-rna-enables-potent-non-viral-in-vivo-genome-editing
#2
Hao Yin, Chun-Qing Song, Sneha Suresh, Qiongqiong Wu, Stephen Walsh, Luke Hyunsik Rhym, Esther Mintzer, Mehmet Fatih Bolukbasi, Lihua Julie Zhu, Kevin Kauffman, Haiwei Mou, Alicia Oberholzer, Junmei Ding, Suet-Yan Kwan, Roman L Bogorad, Timofei Zatsepin, Victor Koteliansky, Scot A Wolfe, Wen Xue, Robert Langer, Daniel G Anderson
Efficient genome editing with Cas9-sgRNA in vivo has required the use of viral delivery systems, which have limitations for clinical applications. Translational efforts to develop other RNA therapeutics have shown that judicious chemical modification of RNAs can improve therapeutic efficacy by reducing susceptibility to nuclease degradation. Guided by the structure of the Cas9-sgRNA complex, we identify regions of sgRNA that can be modified while maintaining or enhancing genome-editing activity, and we develop an optimal set of chemical modifications for in vivo applications...
November 13, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29120819/towards-a-defined-ecm-and-small-molecule-based-monolayer-culture-system-for-the-expansion-of-mouse-and-human-intestinal-stem-cells
#3
Zhixiang Tong, Keir Martyn, Andy Yang, Xiaolei Yin, Benjamin E Mead, Nitin Joshi, Nicholas E Sherman, Robert S Langer, Jeffrey M Karp
Current ISC culture systems face significant challenges such as animal-derived or undefined matrix compositions, batch-to-batch variability (e.g. Matrigel-based organoid culture), and complexity of assaying cell aggregates such as organoids which renders the research and clinical translation of ISCs challenging. Here, through screening for suitable ECM components, we report a defined, collagen based monolayer culture system that supports the growth of mouse and human intestinal epithelial cells (IECs) enriched for an Lgr5(+) population comparable or higher to the levels found in a standard Matrigel-based organoid culture...
October 26, 2017: Biomaterials
https://www.readbyqxmd.com/read/29026204/engineering-and-physical-sciences-in-oncology-challenges-and-opportunities
#4
REVIEW
Michael J Mitchell, Rakesh K Jain, Robert Langer
The principles of engineering and physics have been applied to oncology for nearly 50 years. Engineers and physical scientists have made contributions to all aspects of cancer biology, from quantitative understanding of tumour growth and progression to improved detection and treatment of cancer. Many early efforts focused on experimental and computational modelling of drug distribution, cell cycle kinetics and tumour growth dynamics. In the past decade, we have witnessed exponential growth at the interface of engineering, physics and oncology that has been fuelled by advances in fields including materials science, microfabrication, nanomedicine, microfluidics, imaging, and catalysed by new programmes at the National Institutes of Health (NIH), including the National Institute of Biomedical Imaging and Bioengineering (NIBIB), Physical Sciences in Oncology, and the National Cancer Institute (NCI) Alliance for Nanotechnology...
November 2017: Nature Reviews. Cancer
https://www.readbyqxmd.com/read/28993678/erratum-characterization-of-mechanically-matched-hydrogel-coatings-to-improve-the-biocompatibility-of-neural-implants
#5
Kevin C Spencer, Jay C Sy, Khalil B Ramadi, Ann M Graybiel, Robert Langer, Michael J Cima
A correction to this article has been published and is linked from the HTML version of this paper. The error has not been fixed in the paper.
October 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28991392/ambireactive-r%C3%A2-p-%C3%A2-bh%C3%A2-groups-facilitating-temperature-switchable-bond-activation-by-an-iron-complex
#6
Lisa Vondung, Lars Erik Sattler, Robert Langer
An iron pincer complex containing a hemi-labile (R₃P)₂BH₂-group exhibits temperature-switchable reactivity patterns: a reversible B-H-activation concomitant with a P-B-bond cleavage is observed at room temperature. Below 4 °C intra- and inter-molecular C-H-activation pathways are becoming faster and dominating. Mechanistic investigations reveal that the lability of the (R₃P)₂BH₂-group in combination with the exothermic formation of σ-bonded complexes are responsible for the switchable bond activation...
October 9, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28974673/mechanistic-understanding-of-in-vivo-protein-corona-formation-on-polymeric-nanoparticles-and-impact-on-pharmacokinetics
#7
Nicolas Bertrand, Philippe Grenier, Morteza Mahmoudi, Eliana M Lima, Eric A Appel, Flavio Dormont, Jong-Min Lim, Rohit Karnik, Robert Langer, Omid C Farokhzad
In vitro incubation of nanomaterials with plasma offer insights on biological interactions, but cannot fully explain the in vivo fate of nanomaterials. Here, we use a library of polymer nanoparticles to show how physicochemical characteristics influence blood circulation and early distribution. For particles with different diameters, surface hydrophilicity appears to mediate early clearance. Densities above a critical value of approximately 20 poly(ethylene glycol) chains (MW 5 kDa) per 100 nm(2) prolong circulation times, irrespective of size...
October 3, 2017: Nature Communications
https://www.readbyqxmd.com/read/28963481/synthetic-microparticles-conjugated-with-vegf165-improve-the-survival-of-endothelial-progenitor-cells-via-microrna-17-inhibition
#8
Sezin Aday, Janet Zoldan, Marie Besnier, Laura Carreto, Jaimy Saif, Rui Fernandes, Tiago Santos, Liliana Bernardino, Robert Langer, Costanza Emanueli, Lino Ferreira
Several cell-based therapies are under pre-clinical and clinical evaluation for the treatment of ischemic diseases. Poor survival and vascular engraftment rates of transplanted cells force them to work mainly via time-limited paracrine actions. Although several approaches, including the use of soluble vascular endothelial growth factor (sVEGF)-VEGF165, have been developed in the last 10 years to enhance cell survival, they showed limited efficacy. Here, we report a pro-survival approach based on VEGF-immobilized microparticles (VEGF-MPs)...
September 29, 2017: Nature Communications
https://www.readbyqxmd.com/read/28945422/living-biomaterials
#9
Mark W Tibbitt, Robert Langer
Convergent advances in the fields of synthetic chemistry, soft matter, molecular self-assembly, and the -omics era point to a new generation of synthetic biomaterials that are indistinguishable in form and function from biological matter. Such living biomaterials comprise a "Holy Grail" of the chemical sciences that will transform both modern medicine and materials design.
March 21, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28937662/glucose-responsive-insulin-by-molecular-and-physical-design
#10
Naveed A Bakh, Abel B Cortinas, Michael A Weiss, Robert S Langer, Daniel G Anderson, Zhen Gu, Sanjoy Dutta, Michael S Strano
The concept of a glucose-responsive insulin (GRI) has been a recent objective of diabetes technology. The idea behind the GRI is to create a therapeutic that modulates its potency, concentration or dosing relative to a patient's dynamic glucose concentration, thereby approximating aspects of a normally functioning pancreas. From the perspective of the medicinal chemist, the GRI is also important as a generalized model of a potentially new generation of therapeutics that adjust potency in response to a critical therapeutic marker...
September 22, 2017: Nature Chemistry
https://www.readbyqxmd.com/read/28932622/high-throughput-nuclear-delivery-and-rapid-expression-of-dna-via-mechanical-and-electrical-cell-membrane-disruption
#11
Xiaoyun Ding, Martin Stewart, Armon Sharei, James C Weaver, Robert S Langer, Klavs F Jensen
Nuclear transfection of DNA into mammalian cells is challenging yet critical for many biological and medical studies. Here, by combining cell squeezing and electric-field-driven transport in a device that integrates microfluidic channels with constrictions and microelectrodes, we demonstrate nuclear delivery of plasmid DNA within 1 hour after treatment, the most rapid DNA expression in a high-throughput setting (up to millions of cells per minute per device). Passing cells at high speed through microfluidic constrictions smaller than the cell diameter mechanically disrupts the cell membrane, allowing a subsequent electric field to further disrupt the nuclear envelope and drive DNA molecules into the cytoplasm and nucleus...
2017: Nature biomedical engineering
https://www.readbyqxmd.com/read/28912242/fabrication-of-fillable-microparticles-and-other-complex-3d-microstructures
#12
Kevin J McHugh, Thanh D Nguyen, Allison R Linehan, David Yang, Adam M Behrens, Sviatlana Rose, Zachary L Tochka, Stephany Y Tzeng, James J Norman, Aaron C Anselmo, Xian Xu, Stephanie Tomasic, Matthew A Taylor, Jennifer Lu, Rohiverth Guarecuco, Robert Langer, Ana Jaklenec
Three-dimensional (3D) microstructures created by microfabrication and additive manufacturing have demonstrated value across a number of fields, ranging from biomedicine to microelectronics. However, the techniques used to create these devices each have their own characteristic set of advantages and limitations with regards to resolution, material compatibility, and geometrical constraints that determine the types of microstructures that can be formed. We describe a microfabrication method, termed StampEd Assembly of polymer Layers (SEAL), and create injectable pulsatile drug-delivery microparticles, pH sensors, and 3D microfluidic devices that we could not produce using traditional 3D printing...
September 15, 2017: Science
https://www.readbyqxmd.com/read/28903368/expression-analysis-of-lc3b-and-p62-indicates-intact-activated-autophagy-is-associated-with-an-unfavorable-prognosis-in-colon-cancer
#13
Monique Niklaus, Olivia Adams, Sabina Berezowska, Inti Zlobec, Franziska Graber, Julia Slotta-Huspenina, Ulrich Nitsche, Robert Rosenberg, Mario P Tschan, Rupert Langer
Autophagy is a lysosomal degradation and recycling process implicated in cancer progression and therapy resistance. We assessed the impact of basal autophagy in colon cancer (CC) in vitro and ex vivo. Functional autophagy was demonstrated in CC cell lines (LoVo; HT-29) showing a dose-dependent increase of the autophagy markers LC3B, p62 and autophagic vesciles upon increasing concentrations of the autophagy inhibitor chloroquine, which was demonstrated by immunoblotting, immunofluorescence and electron microscopy...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28893673/extracorporeal-membrane-oxygenation-ecmo-as-salvage-treatment-for-pulmonary-echinococcus-granulosus-infection-with-acute-cyst-rupture
#14
Sören L Becker, Sebastian Fähndrich, Franziska C Trudzinski, Barbara Gärtner, Frank Langer, Torben K Becker, Robert Bals, Philipp M Lepper, Christian Lensch
Extracorporeal membrane oxygenation (ECMO) has been used successfully for the treatment of patients with respiratory failure due to severe infections. Although rare, parasites can also cause severe pulmonary disease. Tapeworms of the genus Echinococcus give rise to the development of cystic structures in the liver, lungs, and other organs. Acute cyst rupture leads to potentially life-threatening infection, and affected patients may deteriorate rapidly. The case of a young woman from Bulgaria who was admitted to hospital with severe dyspnoea, progressive chest pain, and haemoptysis is described...
September 8, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28875984/multiscale-technologies-for-treatment-of-ischemic-cardiomyopathy
#15
REVIEW
Morteza Mahmoudi, Mikyung Yu, Vahid Serpooshan, Joseph C Wu, Robert Langer, Richard T Lee, Jeffrey M Karp, Omid C Farokhzad
The adult mammalian heart possesses only limited capacity for innate regeneration and the response to severe injury is dominated by the formation of scar tissue. Current therapy to replace damaged cardiac tissue is limited to cardiac transplantation and thus many patients suffer progressive decay in the heart's pumping capacity to the point of heart failure. Nanostructured systems have the potential to revolutionize both preventive and therapeutic approaches for treating cardiovascular disease. Here, we outline recent advancements in nanotechnology that could be exploited to overcome the major obstacles in the prevention of and therapy for heart disease...
September 6, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28874662/cre-lox-assisted-non-invasive-in-vivo-tracking-of-specific-cell-populations-by-positron-emission-tomography
#16
Martin Thunemann, Barbara F Schörg, Susanne Feil, Yun Lin, Jakob Voelkl, Matthias Golla, Angelos Vachaviolos, Ursula Kohlhofer, Leticia Quintanilla-Martinez, Marcus Olbrich, Walter Ehrlichmann, Gerald Reischl, Christoph M Griessinger, Harald F Langer, Meinrad Gawaz, Florian Lang, Michael Schäfers, Manfred Kneilling, Bernd J Pichler, Robert Feil
Many pathophysiological processes are associated with proliferation, migration or death of distinct cell populations. Monitoring specific cell types and their progeny in a non-invasive, longitudinal and quantitative manner is still challenging. Here we show a novel cell-tracking system that combines Cre/lox-assisted cell fate mapping with a thymidine kinase (sr39tk) reporter gene for cell detection by positron emission tomography (PET). We generate Rosa26-mT/sr39tk PET reporter mice and induce sr39tk expression in platelets, T lymphocytes or cardiomyocytes...
September 5, 2017: Nature Communications
https://www.readbyqxmd.com/read/28872490/menopausal-hormone-therapy-for-primary-prevention-why-the-uspstf-is-wrong
#17
Robert D Langer, James A Simon, Amos Pines, Rogerio A Lobo, Howard N Hodis, James H Pickar, David F Archer, Philip M Sarrel, Wulf H Utian
No abstract text is available yet for this article.
October 2017: Menopause: the Journal of the North American Menopause Society
https://www.readbyqxmd.com/read/28839004/ventilation-and-neurochemical-changes-during-%C3%A2%C2%B5-opioid-receptor-activation-or-blockade-of-excitatory-receptors-in-the-hypoglossal-motor-nucleus-of-goats
#18
Thomas M Langer, Suzanne E Neumueller, Emma Crumley, Nicholas J Burgraff, Sawan Talwar, Matthew Robert Hodges, Lawrence G Pan, Hubert V Forster
Neuromodulator interdependence posits that changes in one or more neuromodulators are compensated by changes in other modulators to maintain stability in the respiratory control network. Herein, we studied compensatory neuromodulation in the hypoglossal motor nucleus (HMN) after chronic implantation of microtubules unilaterally (n=5) or bilaterally (n=5) into the HMN. After recovery, receptor agonists or antagonists in mock cerebrospinal fluid (mCSF) were dialyzed during the awake and NREM sleep state. During day studies, dialysis of the µ-opioid inhibitory receptor agonist DAMGO (100 µM) decreased pulmonary ventilation (V̇I), breathing frequency (f), and genioglossus (GG) muscle activity, but did not alter neuromodulators measured in the effluent mCSF...
August 24, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28834756/regulation-of-peripheral-myelination-through-transcriptional-buffering-of-egr2-by-an-antisense-long-non-coding-rna
#19
Margot Martinez-Moreno, Timothy Mark O'Shea, John P Zepecki, Alexander Olaru, Jennifer K Ness, Robert Langer, Nikos Tapinos
Precise regulation of Egr2 transcription is fundamentally important to the control of peripheral myelination. Here, we describe a long non-coding RNA antisense to the promoter of Egr2 (Egr2-AS-RNA). During peripheral nerve injury, the expression of Egr2-AS-RNA is increased and correlates with decreased Egr2 transcript and protein levels. Ectopic expression of Egr2-AS-RNA in dorsal root ganglion (DRG) cultures inhibits the expression of Egr2 mRNA and induces demyelination. In vivo inhibition of Egr2-AS-RNA using oligonucleotide GapMers released from a biodegradable hydrogel following sciatic nerve injury reverts the EGR2-mediated gene expression profile and significantly delays demyelination...
August 22, 2017: Cell Reports
https://www.readbyqxmd.com/read/28828455/drug-delivery-by-supramolecular-design
#20
REVIEW
Matthew J Webber, Robert Langer
The rational design of drug delivery approaches leveraging supramolecular chemistry (i.e., "chemistry beyond the molecule") has garnered significant interest in recent years toward improving therapeutics. By using specific, dynamic, and tunable non-covalent interactions, engineered approaches to drug delivery can be realized. Certain benefits to this approach are molecular-level control of composition, improved routes for incorporating and targeting drugs, and new strategies to create delivery devices that respond to a variety of physiologic indicators...
October 30, 2017: Chemical Society Reviews
keyword
keyword
11554
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"