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echinacea in rat

Yutang Wang, Guo Xie, Qian Liu, Xiang Duan, Zhigang Liu, Xuebo Liu
Chicoric acid is a major active constituent of Echinacea purpurea and has a variety of biological functions. In this study, a liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) approach was developed and validated for the determination of chicoric acid in rat plasma and various tissues using ferulic acid as an internal standard (IS). This method was successfully applied to pharmacokinetics, tissue distribution, and plasma protein binding (PPB) study of chicoric acid in Sprague-Dawley (SD) rats dosed with 50mg/kg by gastric gavage...
September 15, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Chunli Gan, Lu Liu, Yan Du, Liqian Wang, Mingjie Gao, Lijun Wu, Chunjuan Yang
A rapid and sensitive assay based on ultra-high performance liquid chromatography with electrospray ionization tandem mass spectrometry was established and validated for the simultaneous determination of cichoric acid, chlorogenic acid, quinic acid, and caffeic acid in rat plasma after oral administration of Echinacea purpurea extract using butylparaben as the internal standard. Samples were pretreated by liquid-liquid extraction with ethyl acetate. The separations for analytes were performed on an ACQUITY UPLC HSS C18 column (1...
May 2016: Journal of Separation Science
Maha Abdelmonem, Samar H Kassem, Hala Gabr, Amira A Shaheen, Tarek Aboushousha
INTRODUCTION: Activation of endogenous stem cell mobilization can contribute to myocardial regeneration after ischemic injury. This study aimed to evaluate the possible role of Avemar or Echinacea extracts in inducing mobilization and homing of CD34(+) stem cells in relation to the inflammatory and hematopoietic cytokines in rats suffering from acute myocardial infarction (AMI). METHODS: AMI was developed by two consecutive subcutaneous injections of isoprenaline (85 mg/kg)...
2015: Stem Cell Research & Therapy
Zeynal Dogan, Bilal Ergul, Murat Sarikaya, Levent Filik, Mehmet Alparslan Gonultaş, Sema Hucumenoglu, Murat Can
Ulcerative colitis (UC) is a chronic disease that causes an inflammatory condition in the colon. Several cytokines, including tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β) and transforming growth factor beta (TGF-β) are crucial components of these inflammatory pathways. New therapeutic strategies are needed for improved clinical outcomes in UC and with less adverse effects. That is why alternative therapies such as herbal remedies are increasingly being used with favorable effects in the treatment of UC...
November 2014: Pakistan Journal of Pharmaceutical Sciences
Nikoletta Jedlinszki, Dóra Rédei, József Haller, Tamás F Freund, Judit Hohmann, István Zupkó
Alkamides are one of the most important constituents of lipophilic extracts of Echinacea angustifolia roots. These compounds play an important role in the versatile pharmacological actions of this plant. The present study aimed to compare the concentrations of isomeric dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamides (DTAI) in brain and periepididymal fat tissues and blood plasma of rats. Thirty, 60, 240 and 720 min after the oral administration of E. angustifolia root extract, tissue and plasma concentrations were determined by reversed-phase HPLC with ESI-MS/MS detection...
June 2014: Natural Product Communications
Z Dogan, B Ergul, M Sarikaya, L Filik, M Alparslan Gonultas, S Hucumenoglu, M Can
BACKGROUND: Ulcerative colitis is a chronic inflammatory condition of the colon, and reactive oxidative metabolites (ROMs) play an important role in its pathogenesis. Alternative therapies such as herbal remedies are increasingly being used in the treatment of ulcerative colitis for better clinical outcome of ulcerative colitis and less adverse effects. Echinacea has many features including antioxidant and wound-healing properties. Hence, the present study was undertaken to evaluate the protective effect of Echinacea spp...
2014: Bratislavské Lekárske Listy
Ling Jiang, Weizu Li, Yuchan Wang, Xiaosu Zhang, Deqiang Yu, Yanyan Yin, Zhongwen Xie, Yi Yuan
Cichoric acid extract (CAE) from Echinacea purpurea L. was used to investigate the anti-arthritic effect by using collagen-induced arthritis (CIA) rat model. The hind paw swelling volume and the body weight were measured and recorded. All the drug solutions were administered orally to rats for a total of 28 days. On day 28, the rats were anaesthetized and decapitated. The thymus and spleen were weighed for the determination of the organ index. The concentration of tumor necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β) and prostaglandin E2 (PGE-2) in the serum was measured using commercially available ELISA kits...
2014: American Journal of Chinese Medicine
Annahita Rezaie, Ali Fazlara, Mojtaba Haghi Karamolah, Ali Shahriari, Hossein Najaf Zadeh, Marzieh Pashmforosh
BACKGROUND: Nitrites are mainly used in food preservation. These materials could change to nitrosamine due to the effect of heat and gastric acid. Nitrosamine is absorbed in intestine and enters the liver and hepatocytes by portal venous system, and hampers the detoxification system of liver by interfering in cytochrome P450 enzymes, so, the liver gently proceeds to cirrhosis and cancer. OBJECTIVES: The current study aimed to investigate the hepatic and renal protective effects of aerial parts of Echinacea purpurea extract (EPE) on injury induced by diethylnitrosamine (DEN)...
2013: Jundishapur Journal of Natural Pharmaceutical Products
Deqiang Yu, Yi Yuan, Ling Jiang, Yuling Tai, Xiumei Yang, Fang Hu, Zhongwen Xie
Echinacea purpurea L. is a medicinal plant originally from North America. It has become a commonly used herbal medicine worldwide because it contains various biologically active compounds. This study was designed to investigate the anti-inflammatory effects of essential oils from E. purpurea in both mice and rats. The extract was obtained from flower of E. purpurea by steam distillation. The anti-inflammatory potential was evaluated in vivo by using different animal models such as xylene-induced mouse ear edema, egg-white-induced rat paw edema, and cotton-induced granuloma tissue proliferating inflammation in mice...
March 2013: Pakistan Journal of Pharmaceutical Sciences
Deniz Uluışık, Ercan Keskin
The aim of the study was to determine the effect of ginseng and echinacea on the mRNA expression of IL-10, TNF-α, and TGF-β1 in healthy rats. Six-week-old male Fischer 344 rats (n = 48) were used. The animals were divided into three equal groups, as follows: control (C); ginseng (G); echinacea (E). While the C group was fed a standard rat diet (Purina) ad libitum for a period of 40 days, the G and E groups animals received the same diet containing 0.5 g/kg of Panax ginseng root powder and 0.75 g/kg of Echinacea purpurea root powder, respectively...
2012: TheScientificWorldJournal
József Haller, Tamás F Freund, Katalin Gyimesi Pelczer, János Füredi, Laszlo Krecsak, János Zámbori
We investigated the toxicity, psychotropic side effects and anxiolytic potential of an Echinacea angustifolia extract that produced promising effects in laboratory tests performed earlier. Rats were studied in the elevated plus-maze, conditioned fear, open-field, object recognition and conditioned place preference tests. Toxicity was studied in rats after intragastric administration. The preparation decreased anxiety in the elevated plus-maze and ameliorated contextual conditioned fear. No lethality or behavioural signs of discomfort were noticed in rats treated with 1000 and 3000 mg/kg Echinacea angustifolia...
January 2013: Phytotherapy Research: PTR
Nadia Ms Arafa, Hayam M Hamuda, Samuel T Melek, Sahar K Darwish
The study aimed to investigate the effect of the oral administration for 15 days of either Echinacea (E) or genuphil (a composite of chondroitin sulphate, glucosamine and methyl sulfonyl methane [GCM]) nutraceutical supplements on female rat model of acute or chronic arthritis induced by bacterial outer membrane protein (OMP) from faecal flora of healthy and rheumatic humans. Anti-cyclic citrullinated peptide (anti-CCP2), C-reactive protein (CRP) and rheumatoid factor (RF) values increased (p < 0.05) in both arthritic groups as compared to normal values...
March 2013: Toxicology and Industrial Health
Sindhura Ramasahayam, Hany N Baraka, Fatma M Abdel Bar, Bilal S Abuasal, Mark P Widrlechner, Khalid A El Sayed, Sharon A Meyer
Echinacea species are used for beneficial effects on immune function, and various prevalent phytochemicals have immunomodulatory effects. Using a commercial E. purpurea (L.) Moench product, we have evaluated the myelopoietic effect on bone marrow of rats treated with various extracts and correlated this with their chemical class composition. Granulocyte/macrophage-colony forming cells (GM-CFCs) from femurs of female Sprague-Dawley rats were assessed at 24 h after 7 daily oral treatments. A 75% ethanolic extract at 50 mg dried weight (derived from 227 mg aerial parts) per kg body weight increased GM-CFCs by 70% but at 100 mg/kg was without effect...
November 2011: Planta Medica
Judit Hohmann, Dóra Rédei, Peter Forgo, Pál Szabó, Tamás F Freund, József Haller, Engin Bojnik, Sándor Benyhe
Multiple chromatographic separations of the CHCl₃-soluble extract of the roots of Echinacea purpurea led to the isolation of 19 compounds. Four natural products, three alkamides and nitidanin diisovalerianate, were identified, and five further compounds were detected for the first time in this species. Additionally, 10 known E. purpurea metabolites were isolated. The structures were determined by mass spectrometry and advanced 1D and 2D NMR techniques. The bioactivity of the isolated compounds was studied in [³⁵S]GTPγS-binding experiments performed on rat brain membrane preparations...
October 2011: Phytochemistry
Norbert Hájos, Noémi Holderith, Beáta Németh, Orsolya I Papp, Gergely G Szabó, Rita Zemankovics, Tamás F Freund, József Haller
Traditionally, Echinacea preparations are used as antiinflammatory agents and immune-enhancers. In addition to these effects, their anxiolytic potency has been recognized recently in laboratory tests. Our aim in this study was to uncover the potential effects of an Echinacea preparation on neuronal operations in the hippocampus, a brain region that is involved in anxiety and anxiety-related behaviors. Using in vitro electrophysiological techniques, we observed that excitatory synaptic transmission in hippocampal slices was significantly suppressed by an Echinacea extract found to be effective in anxiety tests...
March 2012: Phytotherapy Research: PTR
Koji Yamada, Pham Hung, Tae Kyu Park, Pyo Jam Park, Beong Ou Lim
AIM OF THE STUDY: Herbs, as food or medicine, can strengthen the body and increase its resistance to illnesses by acting on various components of the immune system. For example, Echinacea is noted for its ability to enhance immune function, primarily through activation of the innate immune responses. Here, we investigated the potential for two herbs commonly found in India, Ashwagandha (Withania somnifera) and Brahmi (Bacopa monnieri), to enhance immune function and compared their effects to that of Echinacea...
September 1, 2011: Journal of Ethnopharmacology
Karin Ardjomand-Woelkart, Manfred Kollroser, Christoph Magnes, Frank Sinner, Reginald F Frye, Hartmut Derendorf, Rudolf Bauer, Veronika Butterweck
The present study assessed the absolute and relative bioavailabilities of dodeca-2 E,4 E,8 Z,10 E/ Z-tetraenoic acid isobutylamides (tetraenes), the main bioactive constituents in Echinacea, administered as pure compounds or in the form of an Echinacea purpurea root extract preparation. Tetraenes were administered orally by gavage or intravenously in a dose of 0.75 mg/kg. The extract was administered orally in a dose of 158.6 mg/kg which corresponds to the same amount of tetraenes. Pharmacokinetic parameters of tetraenes were calculated by non-compartmental analysis using WinNonlin® 5...
November 2011: Planta Medica
Juliana Mozer Sciani, Bianca Cestari Zychar, Luis Roberto de Camargo Gonçalves, Thiago de Oliveira Nogueira, Renata Giorgi, Daniel Carvalho Pimenta
The sea urchin, Echinometra lucunter, can be found along the Western Central Atlantic shores. In Brazil, it is responsible by circa 50% of the accidents caused by marine animals. The symptoms usually surpass trauma and may be pathologically varied and last differently, ranging from spontaneous healing in a few days, to painful consequences lasting for weeks. In this work, we have mimicked the sea urchin accident by administering an aqueous extract of the spine into mice and rats and evaluated the pathophysiological developments...
March 2011: Experimental Biology and Medicine
Nadia M S Arafa
The study aimed to evaluate the effect Echinacea extract (E) on the testicular antioxidants function in normal rats or that subjected to anti-androgenic compound, cyproterone acetate (CA). Rats were divided into 5 groups treated daily via an oral tube for two intervals 2 and 4 weeks, 1st control, 2nd E (Echinacea treated group in dose 63 mg kg(-1)), 3rd CA (cyproterone acetate treated group in dose 25 mg kg(-1)), 4th E+CA and 5th E as prophylactic one week before E+CA treatment with the same aforementioned E or CA doses...
October 15, 2010: Pakistan Journal of Biological Sciences: PJBS
J Haller, J Hohmann, T F Freund
Echinacea preparations are traditionally used to treat upper respiratory infections and inflammations. No psychotropic effects of Echinacea have been reported so far, although some recently reported active constituents are behaviorally active. Prompted by these findings, the anxiolytic potential of five different Echinacea preparations was evaluated. Three of these decreased anxiety but two of them had a very narrow effective dose range. Only one extract decreased anxiety within a wide dose-range (3-8 mg/kg)...
November 2010: Phytotherapy Research: PTR
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