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Erna Lenters-Westra, Emma English
BACKGROUND: Point-of-care (POC) testing is becoming increasingly valuable in health care delivery, and it is important that the devices used meet the same quality criteria as main laboratory analyzers. While external quality assessment (EQA) provides a great tool for assessing quality, many POC devices are not enrolled in these schemes and standard laboratory evaluations are needed to assess performance. METHODS: The Clinical and Laboratory Standards Institute (CLSI) protocols EP-5 and EP-9 were applied to investigate imprecision, accuracy and bias...
June 1, 2018: Journal of Diabetes Science and Technology
Shirin Olga Eskeland, Erna Moen, Benjamin Hummelen
Trichotillomania, or hair-pulling disorder, is a mental disorder that typically strikes in adolescence and has a chronic course. The condition is frequently accompanied by significant limitations as life unfolds. Comorbidity with depression and anxiety disorders is common.
June 12, 2018: Tidsskrift for Den Norske Lægeforening: Tidsskrift for Praktisk Medicin, Ny Række
Zsolt Fejes, Zsolt Czimmerer, Tibor Szük, Szilárd Póliska, Attila Horváth, Enikő Balogh, Viktória Jeney, Judit Váradi, Ferenc Fenyvesi, György Balla, István Édes, József Balla, János Kappelmayer, Béla Nagy
We previously found higher level of endothelial cell (EC) activation in patients who suffered from in-stent restenosis after bare-metal stenting compared to subjects who underwent drug-eluting stenting (DES) showing no complications. Here we investigated the potential transcriptional and post-transcriptional regulatory mechanisms by which everolimus attenuated EC activation after DES. We studied the effect of everolimus on E-selectin (SELE) and VCAM1 mRNA levels when human coronary artery (HCAECs) and human umbilical vein ECs were challenged with recombinant TNF-α (100 ng/mL) for 1-24 hours in the presence or absence of everolimus using 0...
2018: PloS One
Mehrdad Rakaee, Thomas K Kilvaer, Stig Manfred Dalen, Elin Richardsen, Erna-Elise Paulsen, Sigurd M Hald, Samer Al-Saad, Sigve Andersen, Tom Donnem, Roy M Bremnes, Lill-Tove Busund
The presence of tumor-infiltrating lymphocytes (TILs) positively impacts the outcome of non-small cell lung cancer (NSCLC) patients. Most previous studies have assessed TILs using different immunohistochemical assays. The purpose of this study was to develop and validate a histopathological scoring model for the assessment of TILs in whole tissue hematoxylin and eosin (H&E)-stained section slides of NSCLC patients and to evaluate the model in an immunoscore setting. Therefore, TIL was evaluated manually on H&E slides from 537 surgical specimens of primary resected stage I-III NSCLC patients...
June 6, 2018: Human Pathology
Katsutoshi Imamura, Akiko Takaya, Yo-Ichi Ishida, Yayoi Fukuoka, Toshiki Taya, Ryo Nakaki, Miho Kakeda, Naoto Imamachi, Aiko Sato, Toshimichi Yamada, Rena Onoguchi-Mizutani, Gen Akizuki, Tanzina Tanu, Kazuyuki Tao, Sotaro Miyao, Yutaka Suzuki, Masami Nagahama, Tomoko Yamamoto, Torben Heick Jensen, Nobuyoshi Akimitsu
Cytoplasmic mRNA degradation controls gene expression to help eliminate pathogens during infection. However, it has remained unclear whether such regulation also extends to nuclear RNA decay. Here, we show that 145 unstable nuclear RNAs, including enhancer RNAs (eRNAs) and long noncoding RNAs (lncRNAs) such as NEAT1v2, are stabilized upon Salmonella infection in HeLa cells. In uninfected cells, the RNA exosome, aided by the Nuclear EXosome Targeting (NEXT) complex, degrades these labile transcripts. Upon infection, the levels of the exosome/NEXT components, RRP6 and MTR4, dramatically decrease, resulting in transcript stabilization...
June 7, 2018: EMBO Journal
Noni Novisari Soeroso, Rozaimah Zain-Hamid, Bintang Y M Sinaga, Ahmad Hamim Sadewa, Tamsil Syafiuddin, Elisna Syahruddin, Gino Tann, Erna Mutiara
AIM: This research aimed to analyse the relationship between CYP2A6 gene polymorphism with nicotine dependence and its relation to the number of cigarette consumption among Bataknese smokers. METHOD: This study was a cross-sectional study involving 140 research subjects in Medan, Indonesia. RESULTS: Nicotine dependence rates were found to be significantly associated with the number of cigarette consumption expressed in the Brinkman Index. CONCLUSION: The *1A wild-type alleles have a greater risk of high-very high dependence rate compared to the other variants...
May 20, 2018: Open Access Macedonian Journal of Medical Sciences
Kaja Skjefstad, Charles Johannessen, Thea Grindstad, Thomas Kilvaer, Erna-Elise Paulsen, Mona Pedersen, Tom Donnem, Sigve Andersen, Roy Bremnes, Elin Richardsen, Samer Al-Saad, Lill-Tove Busund
Micro RNAs (miRNA) are small non-coding RNAs that post-transcriptionally regulate gene expression. Dysregulation of miRNA cluster 143/145 has been reported in several malignancies, but their role in non-small cell lung cancer (NSCLC) remains elusive. This study investigates the prognostic impact of miR-143 and miR-145 in primary tumors and metastatic lymph nodes in NSCLC tissue. Tissue from 553 primary tumors and 143 matched metastatic lymph nodes were collected and tissue microarrays were constructed. In situ hybridization was used to evaluate miR-143 and miR-145 expression in tumor epithelial cells and stromal cells in the primary tumors and lymph nodes...
June 4, 2018: Scientific Reports
Flávia Viana Ferreira, Eric Roberto Guimarães Rocha Aguiar, Roenick Proveti Olmo, Karla Pollyanna Vieira de Oliveira, Emanuele Guimarães Silva, Maurício Roberto Viana Sant'Anna, Nelder de Figueiredo Gontijo, Erna Geessien Kroon, Jean Luc Imler, João Trindade Marques
Sandflies are well known vectors for Leishmania but also transmit a number of arthropod-borne viruses (arboviruses). Few studies have addressed the interaction between sandflies and arboviruses. RNA interference (RNAi) mechanisms utilize small non-coding RNAs to regulate different aspects of host-pathogen interactions. The small interfering RNA (siRNA) pathway is a broad antiviral mechanism in insects. In addition, at least in mosquitoes, another RNAi mechanism mediated by PIWI interacting RNAs (piRNAs) is activated by viral infection...
June 4, 2018: PLoS Neglected Tropical Diseases
Izabela Maurício de Rezende, Lívia Sacchetto, Érica Munhoz de Mello, Pedro Augusto Alves, Felipe Campos de Melo Iani, Talita Émile Ribeiro Adelino, Myrian Morato Duarte, Ana Luísa Furtado Cury, André Felipe Leal Bernardes, Tayrine Araujo Santos, Leonardo Soares Pereira, Maria Rita Teixeira Dutra, Dario Brock Ramalho, Benoit de Thoisy, Erna Geessien Kroon, Giliane de Souza Trindade, Betânia Paiva Drumond
BACKGROUND: Yellow fever (YF) is endemic in the Brazilian Amazon Basin, and sporadic outbreaks take place outside the endemic area in Brazil. Since 2016, YF epidemics have been occurring in Southeast Brazil, with more than 1,900 human cases and more than 1,600 epizooties of non-human primates (NHPs) reported until April 2018. Previous studies have demonstrated that Yellow fever virus (YFV) causing outbreaks in 2017 formed a monophyletic group. METHODOLOGY/PRINCIPAL FINDINGS: Aiming to decipher the origin of the YFV responsible for the recent epidemics, we obtained nucleotide sequences of YFV detected in humans (n = 6) and NHPs (n = 10) from Minas Gerais state during 2017-2018...
June 4, 2018: PLoS Neglected Tropical Diseases
Ilmari Pyykkö, Vinaya Manchaiah, Jing Zou, Hilla Levo, Erna Kentala
The purpose of the present study was to characterize the occurrence of Tumarkin attacks in patients with Ménière'sdisease (MD), and to study its association with reported complaints, severity of consequences, and also self-reported work ability. The study used a cross-sectional design. Data obtained from the Finnish Ménière Federation using an electronic survey was analyzed. 602 people with MD responded to the survey (62% response rate) with a mean age of 56.7 years and a mean duration of the disease 12...
May 22, 2018: Journal of Vestibular Research: Equilibrium & Orientation
Erna J Z Krüsemann, Marlou P Lasschuijt, C de Graaf, René A de Wijk, Pieter H Punter, Loes van Tiel, Johannes W J M Cremers, Suzanne van de Nobelen, Sanne Boesveldt, Reinskje Talhout
OBJECTIVES: Tobacco flavours are an important regulatory concept in several jurisdictions, for example in the USA, Canada and Europe. The European Tobacco Products Directive 2014/40/EU prohibits cigarettes and roll-your-own tobacco having a characterising flavour. This directive defines characterising flavour as 'a clearly noticeable smell or taste other than one of tobacco […]'. To distinguish between products with and without a characterising flavour, we trained an expert panel to identify characterising flavours by smelling...
May 23, 2018: Tobacco Control
Erna Johanna Zegerina Krüsemann, Sanne Boesveldt, Kees de Graaf, Reinskje Talhout
Introduction: E-liquids are available in a high variety of flavors. A systematic classification of e-liquid flavors is necessary to increase comparability of research results. In the food, alcohol and fragrance industry, flavors are classified using flavor wheels. We systematically reviewed literature on flavors related to e-cigarette use, to investigate how e-liquid flavors have been classified in research, and propose an e-liquid flavor wheel to classify e-liquids based on marketing descriptions...
May 18, 2018: Nicotine & Tobacco Research: Official Journal of the Society for Research on Nicotine and Tobacco
Yukako Sakaguchi, Keizo Nishikawa, Shigeto Seno, Hideo Matsuda, Hiroshi Takayanagi, Masaru Ishii
Bidirectional transcription has been proposed to play a role associated with enhancer activity. Transcripts called enhancer RNAs (eRNAs) play important roles in gene regulation; however, their roles in osteoclasts are unknown. To analyse eRNAs in osteoclasts comprehensively, we used cap-analysis of gene expression (CAGE) to detect adjacent transcription start sites (TSSs) that were distant from promoters for protein-coding gene expression. When comparing bidirectional TSSs between osteoclast precursors and osteoclasts, we found that bidirectional TSSs were located in the 5'-flanking regions of the Nrp2 and Dcstamp genes...
May 14, 2018: Scientific Reports
Andreia Elisa Cursino, Ana Paula Pessoa Vilela, Ana Paula Moreira Franco-Luiz, Jaquelline Germano de Oliveira, Márcia Furlan Nogueira, João Pessoa Araújo Júnior, Daniel Moura de Aguiar, Erna Geessien Kroon
Equine infectious anemia (EIA) has a worldwide distribution, and is widespread in Brazil. The Brazilian Pantanal presents with high prevalence comprising equine performance and indirectly the livestock industry, since the horses are used for cattle management. Although EIA is routinely diagnosed by the agar gel immunodiffusion test (AGID), this serological assay has some limitations, so PCR-based detection methods have the potential to overcome these limitations and act as complementary tests to those currently used...
May 11, 2018: Archives of Virology
Mads E Hauberg, John F Fullard, Lingxue Zhu, Ariella T Cohain, Claudia Giambartolomei, Ruth Misir, Sarah Reach, Jessica S Johnson, Minghui Wang, Manuel Mattheisen, Anders Dupont Børglum, Bin Zhang, Solveig K Sieberts, Mette A Peters, Enrico Domenici, Eric E Schadt, Bernie Devlin, Pamela Sklar, Kathryn Roeder, Panos Roussos
Transcription at enhancers is a widespread phenomenon which produces so-called enhancer RNA (eRNA) and occurs in an activity-dependent manner. However, the role of eRNA and its utility in exploring disease-associated changes in enhancer function, and the downstream coding transcripts that they regulate, is not well established. We used transcriptomic and epigenomic data to interrogate the relationship of eRNA transcription to disease status and how genetic variants alter enhancer transcriptional activity in the human brain...
May 8, 2018: Molecular Psychiatry
Karen J Parker, Joseph P Garner, Ozge Oztan, Erna R Tarara, Jiang Li, Valentina Sclafani, Laura A Del Rosso, Katie Chun, Sean W Berquist, Michael G Chez, Sonia Partap, Antonio Y Hardan, Elliott H Sherr, John P Capitanio
Autism spectrum disorder (ASD) is a neurodevelopmental condition characterized by core social impairments. ASD remains poorly understood because of the difficulty in studying disease biology directly in patients and the reliance on mouse models that lack clinically relevant, complex social cognition abilities. We use ethological observations in rhesus macaques to identify male monkeys with naturally occurring low sociality. These monkeys showed differences in specific neuropeptide and kinase signaling pathways compared to socially competent male monkeys...
May 2, 2018: Science Translational Medicine
Ma Wan, Brian D Bennett, Gary S Pittman, Michelle R Campbell, Lindsay M Reynolds, Devin K Porter, Christopher L Crowl, Xuting Wang, Dan Su, Neal A Englert, Isabel J Thompson, Yongmei Liu, Douglas A Bell
BACKGROUND: Cigarette smoke is a causal factor in cancers and cardiovascular disease. Smoking-associated differentially methylated regions (SM-DMRs) have been observed in disease studies, but the causal link between altered DNA methylation and transcriptional change is obscure. OBJECTIVE: Our objectives were to finely resolve SM-DMRs and to interrogate the mechanistic link between SM-DMRs and altered transcription of enhancer noncoding RNA (eRNA) and mRNA in human circulating monocytes...
April 27, 2018: Environmental Health Perspectives
Kristin Lara Ólafsdóttir, Helga Jónsdóttir, Nanna Fridriksdóttir, Valgerdur Sigurdardóttir, Erna Haraldsdóttir
BACKGROUND: Advance care planning (ACP) is well recognised as an important component of palliative care. However, there is still a need to explore ways in which it can become a part of routine practice, ensuring a timely and person-centred discussion. OBJECTIVES: To explore patients newly diagnosed with advanced lung cancer and their family members' experiences of engaging in a person-centred and structured ACP discussion facilitated by palliative care nurses in an outpatient oncology clinic at the University Hospital of Iceland...
April 2, 2018: International Journal of Palliative Nursing
Eva Grill, Gülden Akdal, Sandra Becker-Bense, Steffen Hübinger, Doreen Huppert, Erna Kentala, Ralf Strobl, Andreas Zwergal, Nese Celebisoy
PURPOSE: Comprehensive phenotypical data across countries is needed to understand the determinants, prognosis and consequences of vestibular disease. The registry is a data repository for the members of the European DizzyNet. We report results from a pilot study using data from Turkey and Germany. METHODS: The pilot study included a convenience sample of patients aged 18 or above referred to Ege University Medical School Hospital, Dokuz Eylül University Hospital, Izmir, Turkey, and the German Center for German Center for Vertigo and Balance Disorders, University on Munich, Germany, with symptoms of vertigo or dizziness...
April 16, 2018: Journal of Neurology
Sally Boa, Edward Duncan, Erna Haraldsdottir, Sally Wyke
BACKGROUND: Goal setting is recognised as an important way of supporting people to live as actively as possible until death. However, there is little agreement about how goal setting should be handled or delivered by health professionals in everyday practice. AIM: To investigate health-care practitioners' understanding and practice of patient-centred goal setting in a hospice. METHODS: A comparative case study of 10 healthcare practitioners in one hospice...
March 2, 2018: International Journal of Palliative Nursing
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