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https://www.readbyqxmd.com/read/29452366/prevalence-and-incidence-of-anal-and-cervical-high-risk-human-papillomavirus-hpv-types-covered-by-current-hpv-vaccines-among-hiv-infected-women-in-the-study-to-understand-the-natural-history-of-hiv-aids-in-the-era-of-effective-therapy-the-sun-study
#1
Erna Milunka Kojic, Lois Conley, Tim Bush, Susan Cu-Uvin, Elizabeth R Unger, Keith Henry, John Hammer, Gerome Escota, Teresa M Darragh, Joel M Palefsky, John T Brooks, Pragna Patel
Background: Nonavalent (9v) human papilloma virus vaccine targets high-risk (HR)-HPV types 16, 18, 31, 33, 45, 52, 58, and low-risk 6, 11. We examined prevalence, incidence, and clearance of anal and cervical HR-HPV in HIV-infected women. Methods: From 2004-2006, the SUN Study enrolled 167 women from four US cities. Anal and cervical specimens were collected annually for cytology and identification of 37 HPV types; 14 HR include: 9v 16, 18, 31, 33, 45, 52, 58; non-9v 35, 39, 51, 56, 59, 66, 68...
February 14, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29449408/enhancer-rna-profiling-predicts-transcription-factor-activity
#2
Joseph G Azofeifa, Mary A Allen, Josephina R Hendrix, Timothy Read, Jonathan D Rubin, Robin D Dowell
Transcription factors (TFs) exert their regulatory influence through the binding of enhancers, resulting in coordination of gene expression programs. Active enhancers are often characterized by the presence of short, unstable transcripts termed enhancer RNAs (eRNAs). While their function remains unclear, we demonstrate that eRNAs are a powerful readout of TF activity. We infer sites of eRNA origination across hundreds of publicly available nascent transcription data sets and show that eRNAs initiate from sites of TF binding...
February 15, 2018: Genome Research
https://www.readbyqxmd.com/read/29440798/rural-palliative-care-in-north-india-rapid-evaluation-of-a-program-using-a-realist-mixed-method-approach
#3
Daniel F Munday, Erna Haraldsdottir, Manju Manak, Ann Thyle, Cathy M Ratcliff
Context: Palliative care has not developed widely in rural North India. Since 2010, the Emmanuel Hospitals Association (EHA) has been developing a model of palliative care appropriate for this setting, based on teams undertaking home visits with the backup of outpatient and inpatient services. A project to further develop the model operated from 2012 to 2015 supported by funding from the UK. Aims: This study aims to evaluate the EHA palliative care project. Settings and Design: Rapid evaluation method using a mixed method realist approach at the five project hospital sites...
January 2018: Indian Journal of Palliative Care
https://www.readbyqxmd.com/read/29427676/in-vitro-susceptibility-to-st-246-and-cidofovir-corroborates-the-phylogenetic-separation-of-brazilian-vaccinia-virus-into-two-clades
#4
Mariana A Pires, Nathália F S Rodrigues, Danilo B de Oliveira, Felipe L de Assis, Galileu B Costa, Erna G Kroon, Bruno E F Mota
The Orthopoxvirus (OPV) genus of the Poxviridae family contains several human pathogens, including Vaccinia virus (VACV), which have been implicating in outbreaks of a zoonotic disease called Bovine Vaccinia in Brazil. So far, no approved treatment exists for OPV infections, but ST-246 and Cidofovir (CDV) are now in clinical development. Therefore, the objective of this work was to evaluate the susceptibility of five strains of Brazilian VACV (Br-VACV) to ST-246 and Cidofovir. The susceptibility of these strains to both drugs was evaluated by plaque reduction assay, extracellular virus's quantification in the presence of ST-246 and one-step growth curve in cells treated with CDV...
February 7, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29425680/new-coxsackievirus-2apro-and-3cpro-protease-antibodies-for-virus-detection-and-discovery-of-pathogenic-mechanisms
#5
Olli H Laitinen, Emma Svedin, Sebastian Kapell, Minna M Hankaniemi, Pär G Larsson, Erna Domsgen, Virginia M Stone, Juha A E Määttä, Heikki Hyöty, Vesa P Hytönen, Malin Flodström-Tullberg
Enteroviruses (EVs), such as the Coxsackie B-viruses (CVBs), are common human pathogens, which can cause severe disease including meningitis, myocarditis and neonatal sepsis. EVs encode two proteases (2Apro and 3Cpro), which perform the proteolytic cleavage of the CVB polyprotein and also cleave host cell proteins to facilitate viral replication. The 2Apro cause direct damage to the infected heart and tools to investigate 2Apro and 3Cpro expression may contribute new knowledge on virus-induced pathologies. Here, we developed new antibodies to CVB-encoded 2Apro and 3Cpro; Two monoclonal 2Apro antibodies and one 3Cpro antibody were produced...
February 6, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29398462/receiver-operating-characteristic-curve-analysis-of-four-aspergillus-specific-igg-assays-for-the-diagnosis-of-chronic-pulmonary-aspergillosis
#6
Iain D Page, Caroline Baxter, Christophe Hennequin, Malcolm D Richardson, Erna van Hoeyveld, Albert W van Toorenenbergen, David W Denning
Measurement of Aspergillus-specific IgG is central to the diagnosis of chronic pulmonary aspergillosis (CPA), but manufacturers' guidance on test interpretation is based on unpublished data. We performed the first receiver operating characteristic (ROC) area under the curve (AUC) analysis to identify optimal cut-offs for this test in relation to European controls. Aspergillus-specific IgG levels were measured in sera from British adults with CPA and European healthy controls by ImmunoCAP, Immulite, Serion and Bio-Rad assays...
January 6, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29396238/lag-3-in-non-small-cell-lung-cancer-expression-in-primary-tumors-and-metastatic-lymph-nodes-is-associated-with-improved-survival
#7
Sigurd M Hald, Mehrdad Rakaee, Inigo Martinez, Elin Richardsen, Samer Al-Saad, Erna-Elise Paulsen, Egil Støre Blix, Thomas Kilvaer, Sigve Andersen, Lill-Tove Busund, Roy M Bremnes, Tom Donnem
BACKGROUND: Lymphocyte activation gene-3 (LAG-3) is an immune checkpoint receptor and a putative therapeutic target in non-small-cell lung cancer (NSCLC). We explored the prognostic effect of LAG-3+ tumor-infiltrating lymphocytes (TILs) in primary tumors and metastatic lymph nodes in NSCLC and its potential for inclusion in an immunoscore, supplementing the TNM classification. MATERIALS AND METHODS: Primary tumor tissue from 553 stage I-IIIB NSCLC patients and 143 corresponding metastatic lymph nodes were collected...
December 11, 2017: Clinical Lung Cancer
https://www.readbyqxmd.com/read/29395696/client-experiences-with-perinatal-healthcare-for-high-risk-and-low-risk-women
#8
Cherelle M V van Stenus, Magda M Boere-Boonekamp, Erna F G M Kerkhof, Ariana Need
PROBLEM: It is unknown if client experiences with perinatal healthcare differ between low-risk and high-risk women. BACKGROUND: In the Netherlands, risk selection divides pregnant women into low- and high-risk groups. Receiving news that a pregnancy or childbirth has an increased likelihood of complications can cause elevated levels of emotional distress. AIM: The purpose of this study is to describe client experiences with perinatal healthcare and to determine which, if any, background characteristics, pregnancy circumstances, childbirth or follow-up care characteristics are explaining variables of differences in client experiences between high-risk and low-risk women...
January 26, 2018: Women and Birth: Journal of the Australian College of Midwives
https://www.readbyqxmd.com/read/29394906/the-spatial-and-temporal-scales-of-local-dengue-virus-transmission-in-natural-settings-a-retrospective-analysis
#9
Luigi Sedda, Ana Paula Pessoa Vilela, Eric Roberto Guimarães Rocha Aguiar, Caio Henrique Pessoa Gaspar, André Nicolau Aquime Gonçalves, Roenick Proveti Olmo, Ana Teresa Saraiva Silva, Lízia de Cássia da Silveira, Álvaro Eduardo Eiras, Betânia Paiva Drumond, Erna Geessien Kroon, João Trindade Marques
BACKGROUND: Dengue is a vector-borne disease caused by the dengue virus (DENV). Despite the crucial role of Aedes mosquitoes in DENV transmission, pure vector indices poorly correlate with human infections. Therefore there is great need for a better understanding of the spatial and temporal scales of DENV transmission between mosquitoes and humans. Here, we have systematically monitored the circulation of DENV in individual Aedes spp. mosquitoes and human patients from Caratinga, a dengue endemic city in the state of Minas Gerais, in Southeast Brazil...
February 2, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29378788/the-degree-of-enhancer-or-promoter-activity-is-reflected-by-the-levels-and-directionality-of-erna-transcription
#10
Olga Mikhaylichenko, Vladyslav Bondarenko, Dermot Harnett, Ignacio E Schor, Matilda Males, Rebecca R Viales, Eileen E M Furlong
Gene expression is regulated by promoters, which initiate transcription, and enhancers, which control their temporal and spatial activity. However, the discovery that mammalian enhancers also initiate transcription questions the inherent differences between enhancers and promoters. Here, we investigate the transcriptional properties of enhancers during Drosophila embryogenesis using characterized developmental enhancers. We show that while the timing of enhancer transcription is generally correlated with enhancer activity, the levels and directionality of transcription are highly varied among active enhancers...
January 29, 2018: Genes & Development
https://www.readbyqxmd.com/read/29378668/genome-wide-analysis-reveals-a-role-for-tdg-in-estrogen-receptor-mediated-enhancer-rna-transcription-and-3-dimensional-reorganization
#11
Bart Kolendowski, Haider Hassan, Milica Krstic, Majdina Isovic, Gobi Thillainadesan, Ann F Chambers, Alan B Tuck, Joseph Torchia
BACKGROUND: The estrogen receptor (ER) is a ligand-dependant transcription factor expressed in many breast cancers and is the target of many endocrine-based cancer therapies. Genome-wide studies have shown that the ER binds to gene-specific enhancer regions in response to β-estradiol (E2) which undergo transcription producing noncoding enhancer RNA (eRNA). While eRNAs are important for transcriptional activation of neighboring genes, the mechanism remains poorly understood. RESULTS: Using ChIP-Seq we generate a global profile of thymine DNA glycosylase (TDG), an ER coactivator that plays an essential role in DNA demethylation, in response to E2 in the MCF7 breast cancer cell line...
January 29, 2018: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/29368617/ubiquitous-giants-a-plethora-of-giant-viruses-found-in-brazil-and-antarctica
#12
Ana Cláudia Dos S P Andrade, Thalita S Arantes, Rodrigo A L Rodrigues, Talita B Machado, Fábio P Dornas, Melissa F Landell, Cinthia Furst, Luiz G A Borges, Lara A L Dutra, Gabriel Almeida, Giliane de S Trindade, Ivan Bergier, Walter Abrahão, Iara A Borges, Juliana R Cortines, Danilo B de Oliveira, Erna G Kroon, Jônatas S Abrahão
BACKGROUND: Since the discovery of giant viruses infecting amoebae in 2003, many dogmas of virology have been revised and the search for these viruses has been intensified. Over the last few years, several new groups of these viruses have been discovered in various types of samples and environments.In this work, we describe the isolation of 68 giant viruses of amoeba obtained from environmental samples from Brazil and Antarctica. METHODS: Isolated viruses were identified by hemacolor staining, PCR assays and electron microscopy (scanning and/or transmission)...
January 24, 2018: Virology Journal
https://www.readbyqxmd.com/read/29360132/abc-om-f%C3%A3-rmaksflimmer-p%C3%A3-akuten
#13
Ardavan Khoshnood, Erna Gudlaugsdottir, Gisela Lundberg
The ABC of atrial fibrillation at the emergency care department Atrial fibrillation (AF) is the most common tachyarrhythmia. When handling patients with AF at the emergency care department it is of utmost importance to take a structural approach, make the right diagnosis, take care of the risk and/or trigger factors, treat and make sure there is an adequate follow-up.
January 18, 2018: Läkartidningen
https://www.readbyqxmd.com/read/29344345/when-the-drugs-don-t-work-treatment-resistant-schizophrenia-serotonin-and-serendipity
#14
Penelope Lowe, Amir Krivoy, Lilla Porffy, Erna Henriksdottir, Whiskey Eromona, Sukhwinder S Shergill
Treatment-resistant schizophrenia is a serious clinical problem. We adopt a systems-level approach positing a greater role for cognitive control mechanisms in the development of psychotic symptoms and illustrate the clinical application of this via a case report of treatment-resistant patients treated successfully with adjunct pro-cognitive serotonergic medication.
January 2018: Therapeutic Advances in Psychopharmacology
https://www.readbyqxmd.com/read/29339538/e6-protein-expressed-by-high-risk-hpv-activates-super-enhancers-of-the-egfr-and-c-met-oncogenes-by-destabilizing-the-histone-demethylase-kdm5c
#15
Xiaohua Chen, Jun Xian Loo, Xin Shi, Wenjun Xiong, Yong Guo, Haiqiang Ke, Mingkun Yang, Yanping Jiang, Siyu Xia, Min Zhao, Shan Zhong, ChunJiang He, Li Fu, Feng Li
The high-risk (HR) human papillomaviruses (HPV) are causative agents of anogenital tract dysplasia and cancers and a fraction of head and neck cancers. The HR HPV E6 oncoprotein possesses canonical oncogenic functions, such as p53 degradation and telomerase activation. It is also capable of stimulating expression of several oncogenes, but the molecular mechanism underlying these events are poorly understood. Here we provide evidence that HPV16 E6 physically interacts with histone H3K4 demethylase KDM5C, resulting in its degradation in an E3 ligase E6AP- and proteasome-dependent manner...
January 16, 2018: Cancer Research
https://www.readbyqxmd.com/read/29336107/centrality-of-spirituality-religion-in-the-culture-of-palliative-care-service-in-indonesia-an-ethnographic-study
#16
Erna Rochmawati, Rick Wiechula, Kate Cameron
Experiencing life-threatening illness could impact on an individual's spirituality or religious beliefs. In this paper, we report on a study which explored cultural elements that influence the provision of palliative care for people with cancer. A contemporary ethnographic approach was adopted. Observations and interviews were undertaken over 3 months with 48 participants, including palliative care staff, patients, and their families. An ethnographic data analysis framework was adopted to assist in the analysis of data at item, pattern, and structural levels...
January 16, 2018: Nursing & Health Sciences
https://www.readbyqxmd.com/read/29325171/maternal-and-child-health-handbook-use-for-maternal-and-child-care-a-cluster-randomized-controlled-study-in-rural-java-indonesia
#17
Keiko Osaki, Tomoko Hattori, Akemi Toda, Erna Mulati, Lukas Hermawan, Kirana Pritasari, Saptawati Bardosono, Soewarta Kosen
Background: Effectiveness of the Maternal and Child Health Handbook (MCHHB), a home-based booklet for pregnancy, delivery and postnatal/child health, was evaluated on care acquisition and home care in rural Java, a low service-coverage area. Methods: We conducted a health centre-based randomized trial, with a 2-year follow-up. Intervention included (i) MCHHB provision at antenatal care visits; (ii) records and guides by health personnel on and with the MCHHB; and (iii) sensitization of care by volunteers using the MCHHB...
January 9, 2018: Journal of Public Health
https://www.readbyqxmd.com/read/29315434/recognizable-clinical-subtypes-of-osa-across-a-worldwide-sleep-center-population-a-cluster-analysis
#18
Brendan T Keenan, Jinyoung Kim, Bhajan Singh, Lia Bittencourt, Ning-Hung Chen, Peter A Cistulli, Ulysses Magalang, Nigel McArdle, Jesse W Mindel, Bryndis Benediktsdottir, Erna Sif Arnardottir, Lisa Kristin Prochnow, Thomas Penzel, Bernd Sanner, Richard Schwab, Chol Shin, Kate Sutherland, Sergio Tufik, Greg Maislin, Thorarinn Gislason, Allan I Pack
Study Objectives: A recent study of moderate-severe obstructive sleep apnea (OSA) patients in Iceland identified 3 clinical clusters based on symptoms and comorbidities. We sought to verify this finding in a new cohort in Iceland and examine the generalizability of OSA clusters in an international ethnically diverse cohort. Methods: Using data on 972 moderate-severe OSA patients [apnea-hypopnea index (AHI) ≥15 events/hour] recruited from the Sleep Apnea Global Interdisciplinary Consortium (SAGIC) we performed a latent class analysis of 18 self-reported symptom variables, hypertension, cardiovascular disease and diabetes...
January 5, 2018: Sleep
https://www.readbyqxmd.com/read/29306915/screening-for-connective-tissue-disease-associated-antibodies-by-automated-immunoassay
#19
Philippe Willems, Ellen De Langhe, Jolien Claessens, René Westhovens, Erna Van Hoeyveld, Koen Poesen, Steven Vanderschueren, Daniel Blockmans, Xavier Bossuyt
BACKGROUND: Antinuclear antibodies (ANAs) are useful for the diagnosis of ANA-associated systemic rheumatic disease (AASRD). The objective of this study was the evaluation of an immunoassay that detects antibodies to a mixture of 17 antigens as an alternative to indirect immunofluorescence (IIF). METHODS: Nine thousand eight hundred and fifty-six consecutive patients tested for ANAs were tested by IIF and EliA connective tissue disease screen (Thermo-Fisher). Medical records were reviewed for 2475 patients, including all patients that tested positive/equivocal by either test and a selection of 500 patients that tested negative...
January 8, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29301533/recurrence-in-unicentric-castleman-s-disease-postoperatively-a-case-report-and-literature-review
#20
Na Ren, Lei Ding, Erna Jia, Jinru Xue
BACKGROUND: Our case describe a rare recurrence case of Unicentric Castleman's disease (UCD) with hyaline vascular type 14 years after surgery. CASE PRESENTATION: A 35-year-old Chinese female was admitted to hospital with one and half months history chest distress and chest pain. Patient reports a history of thoracic operation for mediastinal mass 14 years ago, and it was diagnosed UCD with hyaline vascular type after postoperative pathological examination. At this time, the imaging examination showed a mediastinal mass once again...
January 4, 2018: BMC Surgery
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