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https://www.readbyqxmd.com/read/28098542/detection-of-vaccinia-virus-in-urban-domestic-cats-brazil
#1
Galileu Barbosa Costa, Júlia Bahia Miranda, Gregório Guilherme Almeida, Jaqueline Silva de Oliveira, Mariana Siqueira Pinheiro, Stefanne Aparecida Gonçalves, Jenner Karlisson Pimenta Dos Reis, Ricardo Gonçalves, Paulo César Peregrino Ferreira, Cláudio Antônio Bonjardim, Jônatas Santos Abrahão, Erna Geessien Kroon, Giliane de Souza Trindade
We investigated possible vaccinia virus (VACV) in urban house cats in Brazil. Serum samples from 6 cats were positive for VACV by PCR, indicating likely VACV circulation among house cats in urban areas of Brazil. This finding highlights the importance of epidemiologic surveillance to avoid outbreaks among urban human populations.
February 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28094574/inferring-a-role-for-methylation-of-intergenic-dna-in-the-regulation-of-genes-aberrantly-expressed-in-precursor-b-cell-acute-lymphoblastic-leukemia
#2
Md Almamun, Olha Kholod, Alexei J Stuckel, Benjamin T Levinson, Nathan T Johnson, Gerald L Arthur, J Wade Davis, Kristen H Taylor
A complete understanding of the mechanisms involved in the development of pre-B ALL is lacking. In this study, we integrated DNA methylation data and gene expression data to elucidate the impact of aberrant intergenic DNA methylation on gene expression in pre-B ALL. We found a subset of differentially methylated intergenic loci that were associated with altered gene expression in pre-B ALL patients. Notably, 84% of these regions were also bound by transcription factors (TF) known to play roles in differentiation and B-cell development in a lymphoblastoid cell line...
January 17, 2017: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/28087167/lingering-questions-about-enhancer-rna-and-enhancer-transcription-coupled-genomic-instability
#3
REVIEW
Gerson Rothschild, Uttiya Basu
Intergenic and intragenic enhancers found inside topologically associated regulatory domains (TADs) express noncoding RNAs, known as enhancer RNAs (eRNAs). Recent studies have indicated these eRNAs play a role in gene regulatory networks by controlling promoter and enhancer interactions and topology of higher-order chromatin structure. Misregulation of enhancer and promoter associated noncoding RNAs (ncRNAs) could stabilize deleterious secondary DNA structures, noncoding RNA associated DNA/RNA hybrid formation, and promote collisions of transcription complexes with replisomes...
January 10, 2017: Trends in Genetics: TIG
https://www.readbyqxmd.com/read/28077249/altered-mucosal-expression-of-micrornas-in-pediatric-patients-with-inflammatory-bowel-disease
#4
Nóra Judit Béres, Zoltán Kiss, Zsófia Sztupinszki, Gábor Lendvai, András Arató, Erna Sziksz, Ádám Vannay, Attila J Szabó, Katalin Eszter Müller, Áron Cseh, Kriszta Boros, Gábor Veres
INTRODUCTION: MicroRNAs (miRs) came recently into focus as promising novel research targets offering new insights into the pathogenesis of inflammatory bowel diseases (IBD). AIMS: The aim of our study was to identify a pediatric IBD (pIBD) characteristic miR profile serving as potential Crohn's disease (CD) and ulcerative colitis (UC) specific diagnostic pattern and to further analyze the related target genes. METHODS: Small RNA sequencing was performed on inflamed and intact colonic biopsies of CD, and control patients...
December 27, 2016: Digestive and Liver Disease
https://www.readbyqxmd.com/read/28064167/potentially-inappropriate-prescribing-to-older-patients-in-primary-care-in-the-netherlands-a-retrospective-longitudinal-study
#5
Linette Bruin-Huisman, Ameen Abu-Hanna, Henk C P M van Weert, Erna Beers
BACKGROUND: potentially inappropriate prescribing (PIP) is associated with adverse health effects in older patients. PIP comprises prescription of potentially inappropriate medications (PIMs) and potential prescribing omissions (PPOs). OBJECTIVE: to estimate the prevalence of PIMs and PPOs among older patients in primary care. DESIGN: retrospective longitudinal study. SETTING: routinely collected data of 182,000 patients of 49 general practitioners (GPs) gathered in the GPs' database of the Academic Medical Center of Amsterdam, the Netherlands...
January 6, 2017: Age and Ageing
https://www.readbyqxmd.com/read/28061024/beyond-the-code-a-guide-to-the-description-and-documentation-of-biodiversity-in-ciliated-protists-alveolata-ciliophora
#6
Alan Warren, David J Patterson, Micah Dunthorn, John C Clamp, Undine E M Achilles-Day, Erna Aescht, Saleh A Al-Farraj, Saleh Al-Quraishy, Khaled Al-Rasheid, Martin Carr, John G Day, Marc Dellinger, Hamed A El-Serehy, Yangbo Fan, Feng Gao, Shan Gao, Jun Gong, Renu Gupta, Xiaozhong Hu, Komal Kamra, Gaytha Langlois, Xiaofeng Lin, Diana Lipscomb, Christopher S Lobban, Pierangelo Luporini, Denis H Lynn, Honggang Ma, Miroslav Macek, Jacqueline Mackenzie-Dodds, Seema Makhija, Robert I Mansergh, Mercedes Martín-Cereceda, Nettie McMiller, David J S Montagnes, Nikolaeva Svetlana, Geoffrey Odhiambo Ong'ondo, Blanca Pérez-Uz, Jasmine Purushothaman, Pablo Quintela-Alonso, Johana Rotterová, Luciana Santoferrara, Chen Shao, Zhuo Shen, Xinlu Shi, Weibo Song, Thorsten Stoeck, Antonietta La Terza, Adriana Vallesi, Mei Wang, Thomas Weisse, Krzysztof Wiackowski, Lei Wu, Kuidong Xu, Zhenzhen Yi, Rebecca Zufall, Sabine Agatha
Recent advances in molecular technology have revolutionized research on all aspects of the biology of organisms, including ciliates, and created unprecedented opportunities for pursuing a more integrative approach to investigation of biodiversity. However, this goal is complicated by large gaps and inconsistencies that still exist in the foundation of basic information about biodiversity of ciliates. The present paper reviews issues relating to the taxonomy of ciliates and presents specific recommendations for best practice in the observation and documentation of their biodiversity...
January 6, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28042078/home-based-palliative-care-for-children-with-incurable-cancer-long-term-perspectives-of-and-impact-on-general-practitioners
#7
Ivana M M van der Geest, Patrick J E Bindels, Saskia M F Pluijm, Erna M C Michiels, Agnes van der Heide, Rob Pieters, Anne-Sophie E Darlington, Marry M van den Heuvel-Eibrink
CONTEXT: Although a large percentage of children with advanced-stage cancer die at home, remarkably little information is available regarding the experience of general practitioners (GPs) with respect to providing home-based palliative care to children with incurable cancer. OBJECTIVES: To explore the perspectives of GPs who care for children with advanced-stage cancer in a home-based setting. METHODS: In this cross-sectional study, 144 GPs who provided home-based palliative care to 150 children with incurable cancer from 2001 through 2010 were invited to complete a questionnaire addressing their perspectives regarding: 1) symptom management, 2) collaboration with other health care professionals, 3) the child's death and care after death and 4) impact of having provided palliative care, scored on distress thermometer (range 0-10)...
December 29, 2016: Journal of Pain and Symptom Management
https://www.readbyqxmd.com/read/28034900/the-sensory-difference-threshold-of-menthol-odor-in-flavored-tobacco-determined-by-combining-sensory-and-chemical-analysis
#8
Erna J Z Krüsemann, Johannes W J M Cremers, Wouter F Visser, Pieter H Punter, Reinskje Talhout
Cigarettes are an often-used consumer product, and flavor is an important determinant of their product appeal. Cigarettes with strong nontobacco flavors are popular among young people, and may facilitate smoking initiation. Discriminating flavors in tobacco is important for regulation purposes, for instance to set upper limits to the levels of important flavor additives. We provide a simple and fast method to determine the human odor difference threshold for flavor additives in a tobacco matrix, using a combination of chemical and sensory analysis...
December 29, 2016: Chemical Senses
https://www.readbyqxmd.com/read/28004878/the-first-isolation-and-characterisation-of-the-protist-labyrinthula-sp-in-southeastern-australia
#9
Brooke K Sullivan, Katie L Robinson, Stacey M Trevathan-Tackett, Erna Lilje, Frank H Gleason, Osu Lilje
As a result of anthropogenic influences and global climate change, emerging infectious marine diseases are thought to be increasingly more common and more severe than in the past. The aim of our investigation was to confirm the presence of Labyrinthula, the etiological agent of the seagrass wasting disease, in Southeastern Australia and provide the first isolation and characterisation of this protist, in Australia. Colonies and individual cells were positively identified as Labyrinthula using published descriptions, diagrams and photographs...
December 22, 2016: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28000722/an-ifih1-gene-polymorphism-associated-with-risk-for-autoimmunity-regulates-canonical-antiviral-defence-pathways-in-coxsackievirus-infected-human-pancreatic-islets
#10
Erna Domsgen, Katharina Lind, Lingjia Kong, Michael H Hühn, Omid Rasool, Frank van Kuppeveld, Olle Korsgren, Riitta Lahesmaa, Malin Flodström-Tullberg
The IFIH1 gene encodes the pattern recognition receptor MDA5. A common polymorphism in IFIH1 (rs1990760, A946T) confers increased risk for autoimmune disease, including type 1-diabetes (T1D). Coxsackievirus infections are linked to T1D and cause beta-cell damage in vitro. Here we demonstrate that the rs1990760 polymorphism regulates the interferon (IFN) signature expressed by human pancreatic islets following Coxsackievirus infection. A strong IFN signature was associated with high expression of IFNλ1 and IFNλ2, linking rs1990760 to the expression of type III IFNs...
December 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27998218/prevalence-and-risk-factors-of-early-endocrine-disorders-in-childhood-brain-tumor-survivors-a-nationwide-multicenter-study
#11
Sarah C Clement, Antoinette Y N Schouten-van Meeteren, Annemieke M Boot, Hedy L Claahsen-van der Grinten, Bernd Granzen, K Sen Han, Geert O Janssens, Erna M Michiels, A S Paul van Trotsenburg, W Peter Vandertop, Dannis G van Vuurden, Leontien C M Kremer, Hubert N Caron, Hanneke M van Santen
Purpose To evaluate the prevalence of, and risk factors for, early endocrine disorders in childhood brain tumor survivors (CBTS). Patients and Methods This nationwide study cohort consisted of 718 CBTS who were diagnosed between 2002 and 2012, and who survived ≥ 2 years after diagnosis. Patients with craniopharyngeoma or a pituitary gland tumor were excluded. Results of all endocrine investigations, which were performed at diagnosis and during follow-up, were collected from patient charts. Multivariable logistic regression was used to study associations between demographic and tumor- and treatment-related variables and the prevalence of early endocrine disorders...
December 20, 2016: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/27983492/meningitis-associated-with-simultaneous-infection-by-multiple-dengue-virus-serotypes-in-children-brazil
#12
Paula Eillanny Silva Marinho, Danilo Bretas de Oliveira, Talitah Michel Sanchez Candiani, Ana Paula Correia Crispim, Pedro Paulo Martins Alvarenga, Fabrizia Cristina Dos Santos Castro, Jonatas Santos Abrahão, Maria Rios, Roney Santos Coimbra, Erna Geessien Kroon
To determine the causes of viral meningitis, we analyzed 22 cerebrospinal fluid samples collected during the 2014-2015 dengue epidemics in Brazil. We identified 3 serotypes of dengue virus (DENV-1, -2, and -3), as well as co-infection with 2 or 3 serotypes. We also detected the Asian II genotype of DENV-2.
January 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/27980063/brd4-promotes-p63-and-grhl3-expression-downstream-of-foxo-in-mammary-epithelial-cells
#13
Sankari Nagarajan, Upasana Bedi, Anusha Budida, Feda H Hamdan, Vivek Kumar Mishra, Zeynab Najafova, Wanhua Xie, Malik Alawi, Daniela Indenbirken, Stefan Knapp, Cheng-Ming Chiang, Adam Grundhoff, Vijayalakshmi Kari, Christina H Scheel, Florian Wegwitz, Steven A Johnsen
Bromodomain-containing protein 4 (BRD4) is a member of the bromo- and extraterminal (BET) domain-containing family of epigenetic readers which is under intensive investigation as a target for anti-tumor therapy. BRD4 plays a central role in promoting the expression of select subsets of genes including many driven by oncogenic transcription factors and signaling pathways. However, the role of BRD4 and the effects of BET inhibitors in non-transformed cells remain mostly unclear. We demonstrate that BRD4 is required for the maintenance of a basal epithelial phenotype by regulating the expression of epithelial-specific genes including TP63 and Grainy Head-like transcription factor-3 (GRHL3) in non-transformed basal-like mammary epithelial cells...
December 15, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27976438/insomnia-complaints-in-lean-patients-with-obstructive-sleep-apnea-negatively-affect-positive-airway-pressure-treatment-adherence
#14
Bjorg Eysteinsdottir, Thorarinn Gislason, Allan I Pack, Bryndís Benediktsdottir, Erna S Arnardottir, Samuel T Kuna, Erla Björnsdottir
The objective of this study was to evaluate the determinants of long-term adherence to positive airway pressure treatment among patients with obstructive sleep apnea, with special emphasis on patients who stop positive airway pressure treatment within 1 year. This is a prospective long-term follow-up of subjects in the Icelandic Sleep Apnea Cohort who were diagnosed with obstructive sleep apnea between 2005 and 2009, and started on positive airway pressure treatment. In October 2014, positive airway pressure adherence was obtained by systematically evaluating available clinical files (n = 796; 644 males, 152 females) with moderate to severe obstructive sleep apnea (apnea-hypopnea index ≥15 events per h)...
December 15, 2016: Journal of Sleep Research
https://www.readbyqxmd.com/read/27973399/serro-2-virus-highlights-the-fundamental-genomic-and-biological-features-of-a-natural-vaccinia-virus-infecting-humans
#15
Giliane de Souza Trindade, Ginny L Emerson, Scott Sammons, Michael Frace, Dhwani Govil, Bruno Eduardo Fernandes Mota, Jônatas Santos Abrahão, Felipe Lopes de Assis, Melissa Olsen-Rasmussen, Cynthia S Goldsmith, Yu Li, Darin Carroll, Flavio Guimarães da Fonseca, Erna Kroon, Inger K Damon
Vaccinia virus (VACV) has been implicated in infections of dairy cattle and humans, and outbreaks have substantially impacted local economies and public health in Brazil. During a 2005 outbreak, a VACV strain designated Serro 2 virus (S2V) was collected from a 30-year old male milker. Our aim was to phenotypically and genetically characterize this VACV Brazilian isolate. S2V produced small round plaques without associated comets when grown in BSC40 cells. Furthermore, S2V was less virulent than the prototype strain VACV-Western Reserve (WR) in a murine model of intradermal infection, producing a tiny lesion with virtually no surrounding inflammation...
December 10, 2016: Viruses
https://www.readbyqxmd.com/read/27966000/the-differential-effects-of-fty720-on-functional-recovery-and-infarct-size-following-myocardial-ischaemia-reperfusion
#16
Derick van Vuuren, Erna Marais, Sonia Genade, Amanda Lochner
AIM: The aim of this study was to evaluate the effects of the sphingosine analogue, FTY720 (Fingolimod), on the outcomes of myocardial ischaemia/reperfusion (I/R) injury. METHODS: Two concentrations of FTY720 (1 or 2.5 µM were administered either prior to (PreFTY), or following (PostFTY) 20 minutes' global (GI) or 35 minutes' regional ischaemia (RI) in the isolated, perfused, working rat heart. Functional recovery during reperfusion was assessed following both models of ischaemia, while infarct size (IFS) was determined following RI...
November 2016: Cardiovascular Journal of Africa
https://www.readbyqxmd.com/read/27932455/single-cell-profiling-reveals-that-erna-accumulation-at-enhancer-promoter-loops-is-not-required-to-sustain-transcription
#17
Samir Rahman, Cornelia E Zorca, Tatiana Traboulsi, Emmanuel Noutahi, Matthew R Krause, Sylvie Mader, Daniel Zenklusen
Enhancers are intergenic DNA elements that regulate the transcription of target genes in response to signaling pathways by interacting with promoters over large genomic distances. Recent studies have revealed that enhancers are bi-directionally transcribed into enhancer RNAs (eRNAs). Using single-molecule fluorescence in situ hybridization (smFISH), we investigated the eRNA-mediated regulation of transcription during estrogen induction in MCF-7 cells. We demonstrate that eRNAs are localized exclusively in the nucleus and are induced with similar kinetics as target mRNAs...
December 8, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27892961/host-derived-extracellular-rna-promotes-adhesion-of-streptococcus-pneumoniae-to-endothelial-and-epithelial-cells
#18
Dariusz Zakrzewicz, Simone Bergmann, Miroslava Didiasova, Benedetto Daniele Giaimo, Tilman Borggrefe, Maren Mieth, Andreas C Hocke, Guenter Lochnit, Liliana Schaefer, Sven Hammerschmidt, Klaus T Preissner, Malgorzata Wygrecka
Streptococcus pneumoniae is the most frequent cause of community-acquired pneumonia. The infection process involves bacterial cell surface receptors, which interact with host extracellular matrix components to facilitate colonization and dissemination of bacteria. Here, we investigated the role of host-derived extracellular RNA (eRNA) in the process of pneumococcal alveolar epithelial cell infection. Our study demonstrates that eRNA dose-dependently increased S. pneumoniae invasion of alveolar epithelial cells...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27884889/palliative-care-in-children-with-cancer-implications-for-general-practice
#19
EDITORIAL
Ivana Mm van der Geest, Patrick Je Bindels, Saskia Mf Pluijm, Erna Mc Michiels, Agnes van der Heide, Rob Pieters, Anne-Sophie E Darlington, Marry M van den Heuvel-Eibrink
No abstract text is available yet for this article.
December 2016: British Journal of General Practice: the Journal of the Royal College of General Practitioners
https://www.readbyqxmd.com/read/27869601/detection-of-vaccinia-virus-in-dairy-cattle-serum-samples-from-2009-uruguay
#20
Ana Paula Moreira Franco-Luiz, Danilo Bretas Oliveira, Alexandre Fagundes Pereira, Mirela Cristina Soares Gasparini, Cláudio Antônio Bonjardim, Paulo César Peregrino Ferreira, Giliane de Souza Trindade, Rodrigo Puentes, Agustin Furtado, Jônatas Santos Abrahão, Erna Geessien Kroon
We detected orthopoxvirus in 28 of 125 serum samples collected during 2009 from cattle in Uruguay. Two samples were PCR-positive for vaccinia virus and had sequences similar to those for vaccinia virus associated with outbreaks in Brazil. Autochthonous circulation of vaccinia virus in Uruguay and other South American countries cannot be ruled out.
December 2016: Emerging Infectious Diseases
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