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https://www.readbyqxmd.com/read/28640813/identification-of-a-sj%C3%A3-gren-s-syndrome-susceptibility-locus-at-oas1-that-influences-isoform-switching-protein-expression-and-responsiveness-to-type-i-interferons
#1
He Li, Tove Ragna Reksten, John A Ice, Jennifer A Kelly, Indra Adrianto, Astrid Rasmussen, Shaofeng Wang, Bo He, Kiely M Grundahl, Stuart B Glenn, Corinne Miceli-Richard, Simon Bowman, Sue Lester, Per Eriksson, Maija-Leena Eloranta, Johan G Brun, Lasse G Gøransson, Erna Harboe, Joel M Guthridge, Kenneth M Kaufman, Marika Kvarnström, Deborah S Cunninghame Graham, Ketan Patel, Adam J Adler, A Darise Farris, Michael T Brennan, James Chodosh, Rajaram Gopalakrishnan, Michael H Weisman, Swamy Venuturupalli, Daniel J Wallace, Kimberly S Hefner, Glen D Houston, Andrew J W Huang, Pamela J Hughes, David M Lewis, Lida Radfar, Evan S Vista, Contessa E Edgar, Michael D Rohrer, Donald U Stone, Timothy J Vyse, John B Harley, Patrick M Gaffney, Judith A James, Sean Turner, Ilias Alevizos, Juan-Manuel Anaya, Nelson L Rhodus, Barbara M Segal, Courtney G Montgomery, R Hal Scofield, Susan Kovats, Xavier Mariette, Lars Rönnblom, Torsten Witte, Maureen Rischmueller, Marie Wahren-Herlenius, Roald Omdal, Roland Jonsson, Wan-Fai Ng, Gunnel Nordmark, Christopher J Lessard, Kathy L Sivils
Sjögren's syndrome (SS) is a common, autoimmune exocrinopathy distinguished by keratoconjunctivitis sicca and xerostomia. Patients frequently develop serious complications including lymphoma, pulmonary dysfunction, neuropathy, vasculitis, and debilitating fatigue. Dysregulation of type I interferon (IFN) pathway is a prominent feature of SS and is correlated with increased autoantibody titers and disease severity. To identify genetic determinants of IFN pathway dysregulation in SS, we performed cis-expression quantitative trait locus (eQTL) analyses focusing on differentially expressed type I IFN-inducible transcripts identified through a transcriptome profiling study...
June 22, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28637776/targeting-of-extracellular-rna-reduces-edema-formation-and-infarct-size-and-improves-survival-after-myocardial-infarction-in-mice
#2
Philipp Stieger, Jan-Marcus Daniel, Christiane Thölen, Jochen Dutzmann, Kai Knöpp, Dursun Gündüz, Muhammad Aslam, Marian Kampschulte, Alexander Langheinrich, Silvia Fischer, Hector Cabrera-Fuentes, Yong Wang, Kai C Wollert, Johann Bauersachs, Rüdiger Braun-Dullaeus, Klaus T Preissner, Daniel G Sedding
BACKGROUND: Following myocardial infarction (MI), peri-infarct myocardial edema formation further impairs cardiac function. Extracellular RNA (eRNA) released from injured cells strongly increases vascular permeability. This study aimed to assess the role of eRNA in MI-induced cardiac edema formation, infarct size, cardiac function, and survival after acute MI and to evaluate the therapeutic potential of ribonuclease 1 (RNase-1) treatment as an eRNA-degrading intervention. METHODS AND RESULTS: C57BL/6J mice were subjected to MI by permanent ligation of the left anterior descending coronary artery...
June 21, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28635496/internet-based-peer-support-for-m%C3%A3-ni%C3%A3-re-s-disease-a-summary-of-web-based-data-collection-impact-evaluation-and-user-evaluation
#3
Ilmari Pyykkő, Vinaya Manchaiah, Hilla Levo, Erna Kentala, Martti Juhola
OBJECTIVE: This paper presents a summary of web-based data collection, impact evaluation, and user evaluations of an Internet-based peer support program for Ménière's disease (MD). DESIGN: The program is written in html-form. The data are stored in a MySQL database and uses machine learning in the diagnosis of MD. The program works interactively with the user and assesses the participant's disorder profile in various dimensions (i.e., symptoms, impact, personal traits, and positive attitude)...
February 9, 2017: International Journal of Audiology
https://www.readbyqxmd.com/read/28634494/antiviral-activity-of-fridericia-formosa-bureau-l-g-lohmann-bignoniaceae-extracts-and-constituents
#4
Geraldo Célio Brandão, Erna G Kroon, José D Souza Filho, Alaíde Braga Oliveira
A phytochemical study of Fridericia formosa (Bignoniaceae) ethanol extracts of leaves, stems, and fruits was guided by in vitro assays against vaccinia virus Western Reserve (VACV-WR), human herpes virus 1 (HSV-1), murine encephalomyocarditis virus (EMCV), and dengue virus type 2 (DENV-2) by the MTT method. All the ethanol extracts were active against DENV-2, HSV-1, and VACV-WR with best results for the fruits extract against DENV-2 (SI > 38.2). For VACV-WR and HSV-1, EC50 values > 200 μg mL(-1) were determined, while no inhibition of the cytopathic effect was observed with EMCV...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28626832/fragment-binding-to-%C3%AE-secretase-1-without-catalytic-aspartate-interactions-identified-via-orthogonal-screening-approaches
#5
Frederik J R Rombouts, Richard Alexander, Erna Cleiren, Alex De Groot, Michel Carpentier, Joyce Dijkmans, Katleen Fierens, Stefan Masure, Diederik Moechars, Martina Palomino-Schätzlein, Antonio Pineda-Lucena, Andrés A Trabanco, Daan Van Glabbeek, Ann Vos, Gary Tresadern
An approach to identify β-secretase 1 (BACE1) fragment binders that do not interact with the catalytic aspartate dyad is presented. A ThermoFluor (thermal shift) and a fluorescence resonance energy transfer enzymatic screen on the soluble domain of BACE1, together with a surface plasmon resonance (SPR) screen on the soluble domain of BACE1 and a mutant of one catalytic Asp (D32N), were run in parallel. Fragments that were active in at least two of these assays were further confirmed using one-dimensional NMR (WaterLOGSY) and SPR binding competition studies with peptidic inhibitor OM99-2...
February 28, 2017: ACS Omega
https://www.readbyqxmd.com/read/28624445/prevalence-and-predictors-of-chronic-pain-in-pregnancy-and-postpartum
#6
Allana Munro, Ronald B George, Jill Chorney, Erna Snelgrove-Clarke, Natalie O Rosen
OBJECTIVE: A clinically relevant number of patients report pain 1 year after vaginal delivery or Caesarean delivery. Study objectives were to identify the incidence of peripartum pain; determine whether pre-existing pain, pregnancy pain, or pain 2 weeks postpartum predicts pain at 3 months; and to identify whether delivery mode, epidural analgesia use, or delivery complications predict non-genito-pelvic pain postpartum. METHODS: Primiparous women at 30 to 36 weeks GA with an uncomplicated singleton pregnancy were recruited from a large perinatal clinic...
June 14, 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/28624225/inhibition-of-mef2a-enhances-neovascularization-via-post-transcriptional-regulation-of-14q32-micrornas-mir-329-and-mir-494
#7
Sabine M J Welten, Margreet R de Vries, Erna A B Peters, Sudhir Agrawal, Paul H A Quax, A Yaël Nossent
Improving the efficacy of neovascularization is a promising strategy to restore perfusion of ischemic tissues in patients with peripheral arterial disease. The 14q32 microRNA cluster is highly involved in neovascularization. The Mef2a transcription factor has been shown to induce transcription of the microRNAs within this cluster. We inhibited expression of Mef2a using gene-silencing oligonucleotides (GSOs) in an in vivo hind limb ischemia model. Treatment with GSO-Mef2a clearly improved blood flow recovery within 3 days (44% recovery versus 25% recovery in control) and persisted until 14 days after ischemia induction (80% recovery versus 60% recovery in control)...
June 16, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28620810/c-jun-integrates-signals-from-both-mek-erk-and-mkk-jnk-pathways-upon-vaccinia-virus-infection
#8
Flávia G G Leite, Alice A Torres, Leonardo C De Oliveira, André F P Da Cruz, Jamária A P Soares-Martins, Anna C T C Pereira, Giliane S Trindade, Jonatas S Abrahão, Erna G Kroon, Paulo C P Ferreira, Cláudio A Bonjardim
Usurpation of the host's signalling pathways is a common strategy employed by viruses to promote their successful replication. Here we show that infection with the orthopoxvirus vaccinia virus (VACV) leads to sustained stimulation of c-Jun activity during the entire infective cycle. This stimulation is temporally regulated through MEK/ERK or MKK/JNK pathways, i.e. during the early/mid phase (1 to 6 hpi) and in the late phase (9 to 24 hpi) of the infective cycle, respectively. As a transcriptional regulator, upon infection with VACV, c-Jun is translocated from the cytoplasm to the nucleus, where it binds to the AP-1 DNA sequence found at the promoter region of its target genes...
June 15, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28604233/distance-education-methods-are-useful-for-delivering-education-to-palliative-caregivers-a-single-arm-trial-of-an-education-package-palliative-caregivers-education-package
#9
Liz Forbat, Rowena Robinson, Rachel Bilton-Simek, Karemah Francois, Marsha Lewis, Erna Haraldsdottir
BACKGROUND: Face-to-face/group education for palliative caregivers is successful, but relies on caregivers travelling, being absent from the patient, and rigid timings. This presents inequities for those in rural locations. AIM: To design and test an innovative distance-learning educational package (PrECEPt: PalliativE Caregivers Education Package). DESIGN: Single-arm mixed-method feasibility proof-of-concept trial (ACTRN12616000601437). The primary outcome was carer self-efficacy, with secondary outcomes focused on caregiver preparedness and carer tasks/needs...
June 1, 2017: Palliative Medicine
https://www.readbyqxmd.com/read/28599852/non-coding-transcripts-from-enhancers-new-insights-into-enhancer-activity-and-gene-expression-regulation
#10
REVIEW
Hongjun Chen, Guangshi Du, Xu Song, Ling Li
Long non-coding RNAs (lncRNAs) have gained widespread interest in the past decade owing to their enormous amount and surprising functions implicated in a variety of biological processes. Some lncRNAs exert function as enhancers, i.e., activating gene transcription by serving as the cis-regulatory molecules. Furthermore, recent studies have demonstrated that many enhancer elements can be transcribed and produce RNA molecules, which are termed as enhancer RNAs (eRNAs). The eRNAs are not merely the by-product of the enhancer transcription...
June 6, 2017: Genomics, Proteomics & Bioinformatics
https://www.readbyqxmd.com/read/28590231/evaluation-of-phenolic-profile-and-antibacterial-bioactivities-of-nigella-sativa-l-seed-extracts
#11
Anela Topcagic, Sanja Cavar Zeljkovic, Erna Karalija, Semira Galijasevic, Emin Sofic
Black cumin (Nigella sativa L. [N. sativa]) seed extracts demonstrated numerous beneficial biological effects including, among others, antidiabetic, anticancer, immunomodulatory, antimicrobial, anti-inflammatory, antihypertensive, and antioxidant activity. To better understand the phytochemical composition of N. sativa seeds, methanol seed extracts were analyzed for phenolic acid and flavonoid content. Furthermore, we tested N. sativa methanol, n-hexane, and aqueous seed extracts for their inhibitory activity against anti-butyrylcholinesterase (BChE) and catalase (CAT) as well as for antimicrobial activity against several bacteria...
June 7, 2017: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28588641/the-significant-pathways-and-genes-underlying-the-colon-cancer-treatment-by-the-traditional-chinese-medicine-phy906
#12
Ziyuan Su, Changyu Zhou, Shaoyou Qin, Erna Jia, Zhenting Wu
BACKGROUND: We attempted to explore the molecular mechanism underlying PHY906 intervention of colon cancer. METHODS: The microarray data of tumors treated by PHY906 and PBS alone were downloaded from the public Gene Expression Omnibus database. The dataset was further analyzed for the differentially expressed genes (DEGs) and their related biological functions were analyzed, followed by function and pathways. Protein-protein interaction (PPI) network was constructed and the significant nodes were screened by network centralities and then the significant modules analysis...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28581208/pigmentation-more-than-one-master
#13
Erna Magnúsdóttir
Vertebrate pigmentation is a process directed by various transcriptional regulators, that drive the specification of melanoblasts from neural crest cells, their proliferation and migration, and terminal differentiation into melanocytes, culminating in the generation of pigment and it's distribution to surrounding keratinocytes. The transcription factor MITF has been coined the "master regulator" of melanocyte development (reviewed in (Steingrímsson, Copeland and Jenkins, 2004)), as it drives both the specification and differentiation of melanocytes...
June 5, 2017: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/28561630/-nurse-style-of-central-vein-our-experience-in-the-peripherally-inserted-central-venous-catheter
#14
Gábor Kollár, Hussain Alizadeh, Erna Gulyás
What is PICC line insertion? The PICC is a soft, flexible catheter which is made of polyurethane or silicone, and is inserted via an upper or lower extremity peripheral vein into superior or inferior vena cava. The origin of PICC line dates back to the early 1950s. Since the introduction of the PICC catheter, this method of venous catheterization has gone through many changes as regards the technique of insertion or the type of catheter used. Despite the routine use of PICC line worldwide, little progress has been made in its use in Hungary...
June 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/28545094/economic-evaluation-of-policy-options-for-dialysis-in-end-stage-renal-disease-patients-under-the-universal-health-coverage-in-indonesia
#15
Afiatin, Levina Chandra Khoe, Erna Kristin, Lusiana Siti Masytoh, Eva Herlinawaty, Pitsaphun Werayingyong, Mardiati Nadjib, Sudigdo Sastroasmoro, Yot Teerawattananon
OBJECTIVES: This study aims to assess the value for money and budget impact of offering hemodialysis (HD) as a first-line treatment, or the HD-first policy, and the peritoneal dialysis (PD) first policy compared to a supportive care option in patients with end-stage renal disease (ESRD) in Indonesia. METHODS: A Markov model-based economic evaluation was performed using local and international data to quantify the potential costs and health-related outcomes in terms of life years (LYs) and quality-adjusted life years (QALYs)...
2017: PloS One
https://www.readbyqxmd.com/read/28533985/metabarcoding-monitoring-analysis-the-pros-and-cons-of-using-co-extracted-environmental-dna-and-rna-data-to-assess-offshore-oil-production-impacts-on-benthic-communities
#16
Olivier Laroche, Susanna A Wood, Louis A Tremblay, Gavin Lear, Joanne I Ellis, Xavier Pochon
Sequencing environmental DNA (eDNA) is increasingly being used as an alternative to traditional morphological-based identification to characterize biological assemblages and monitor anthropogenic impacts in marine environments. Most studies only assess eDNA which, compared to eRNA, can persist longer in the environment after cell death. Therefore, eRNA may provide a more immediate census of the environment due to its relatively weaker stability, leading some researchers to advocate for the use of eRNA as an additional, or perhaps superior proxy for portraying ecological changes...
2017: PeerJ
https://www.readbyqxmd.com/read/28533025/enhancer-derived-rna-a-primer
#17
REVIEW
Feng Liu
Enhancer-derived RNAs (eRNAs) are a group of RNAs transcribed by RNA polymerase II from the domain of transcription enhancers, a major type of cis-regulatory elements in the genome. The correlation between eRNA production and enhancer activity has stimulated studies on the potential role of eRNAs in transcriptional regulation. Additionally, eRNA has also served as a marker for global identification of enhancers. Here I review the brief history and fascinating properties of eRNAs.
May 19, 2017: Genomics, Proteomics & Bioinformatics
https://www.readbyqxmd.com/read/28518030/serologic-and-molecular-evidence-of-vaccinia-virus-circulation-among-small-mammals-from-different-biomes-brazil
#18
Júlia B Miranda, Iara A Borges, Samantha P S Campos, Flávia N Vieira, Tatiana M F de Ázara, Fernanda A Marques, Galileu B Costa, Ana Paula M F Luis, Jaqueline S de Oliveira, Paulo César P Ferreira, Cláudio Antônio Bonjardim, Silvio L M da Silva, Álvaro E Eiras, Jônatas S Abrahão, Erna G Kroon, Betânia P Drumond, Adriano P Paglia, Giliane de S Trindade
Vaccinia virus (VACV) is a zoonotic agent that causes a disease called bovine vaccinia, which is detected mainly in milking cattle and humans in close contact with these animals. Even though many aspects of VACV infection have been described, much is still unknown about its circulation in the environment and its natural hosts/reservoirs. To investigate the presence of Orthopoxvirus antibodies or VACV DNA, we captured small rodents and marsupials in 3 areas of Minas Gerais state, Brazil, and tested their samples in a laboratory...
June 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28516314/harmonic-subtraction-for-evaluating-right-ventricle-ejection-fraction-from-planar-equilibrium-radionuclide-angiography
#19
Dacian V Bonta, John N Aarsvold, Sandra F Grant, Naomi P Alazraki
We report an initial investigation of a subtraction-based method to estimate right ventricle ejection fraction (RVEF) from ECG-gated planar equilibrium radionuclide angiography (ERNA) data. Twenty-six consecutive patients referred for scintigraphic evaluation of cardiac function prior to chemotherapy had ECG-gated first-pass (FP) imaging and ERNA imaging performed following the same radiotracer injection. RVEF was computed from FP images (RVEFFP) and separately from ERNA images (RVEFERNA). Standard methods for computing ejection fractions were used to obtain RVEFFP values...
May 17, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28513985/self-assembled-nanofiber-coatings-for-controlling-cell-responses
#20
Raquel C Barros, Edith Gelens, Erna Bulten, Annemarie Tuin, Menno R de Jong, Roel Kuijer, Theo G van Kooten
Nanofibers are thought to enhance cell adhesion, growth, and function. We demonstrate that the choice of building blocks in self-assembling nanofiber systems can be used to control cell behavior. The use of 2 D-coated, self-assembled nanofibers in controlling lens epithelial cells, fibroblasts, and mesenchymal stem cells was investigated, focusing on gene and protein expression related to the fibrotic response. To this end, three nanofibers with different characteristics (morphology, topography, and wettability) were compared with two standard materials frequently used in culturing cells, TCPS, and a collagen type I coating...
May 17, 2017: Journal of Biomedical Materials Research. Part A
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