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https://www.readbyqxmd.com/read/27892961/host-derived-extracellular-rna-promotes-adhesion-of-streptococcus-pneumoniae-to-endothelial-and-epithelial-cells
#1
Dariusz Zakrzewicz, Simone Bergmann, Miroslava Didiasova, Benedetto Daniele Giaimo, Tilman Borggrefe, Maren Mieth, Andreas C Hocke, Guenter Lochnit, Liliana Schaefer, Sven Hammerschmidt, Klaus T Preissner, Malgorzata Wygrecka
Streptococcus pneumoniae is the most frequent cause of community-acquired pneumonia. The infection process involves bacterial cell surface receptors, which interact with host extracellular matrix components to facilitate colonization and dissemination of bacteria. Here, we investigated the role of host-derived extracellular RNA (eRNA) in the process of pneumococcal alveolar epithelial cell infection. Our study demonstrates that eRNA dose-dependently increased S. pneumoniae invasion of alveolar epithelial cells...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27884889/palliative-care-in-children-with-cancer-implications-for-general-practice
#2
EDITORIAL
Ivana Mm van der Geest, Patrick Je Bindels, Saskia Mf Pluijm, Erna Mc Michiels, Agnes van der Heide, Rob Pieters, Anne-Sophie E Darlington, Marry M van den Heuvel-Eibrink
No abstract text is available yet for this article.
December 2016: British Journal of General Practice: the Journal of the Royal College of General Practitioners
https://www.readbyqxmd.com/read/27869601/detection-of-vaccinia-virus-in-dairy-cattle-serum-samples-from-2009-uruguay
#3
Ana Paula Moreira Franco-Luiz, Danilo Bretas Oliveira, Alexandre Fagundes Pereira, Mirela Cristina Soares Gasparini, Cláudio Antônio Bonjardim, Paulo César Peregrino Ferreira, Giliane de Souza Trindade, Rodrigo Puentes, Agustin Furtado, Jônatas Santos Abrahão, Erna Geessien Kroon
We detected orthopoxvirus in 28 of 125 serum samples collected during 2009 from cattle in Uruguay. Two samples were PCR-positive for vaccinia virus and had sequences similar to those for vaccinia virus associated with outbreaks in Brazil. Autochthonous circulation of vaccinia virus in Uruguay and other South American countries cannot be ruled out.
December 2016: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/27865922/low-frequency-cortical-oscillations-are-modulated-by-temporal-prediction-and-temporal-error-coding
#4
Louise Catheryne Barne, Peter Maurice Erna Claessens, Marcelo Bussotti Reyes, Marcelo Salvador Caetano, André Mascioli Cravo
Monitoring and updating temporal predictions are critical abilities for adaptive behavior. Here, we investigated whether neural oscillations are related to violation and updating of temporal predictions. Human participants performed an experiment in which they had to generate a target at an expected time point, by pressing a button while taking into account a variable delay between the act and the stimulus occurrence. Our behavioral results showed that participants quickly adapted their temporal predictions in face of an error...
November 16, 2016: NeuroImage
https://www.readbyqxmd.com/read/27865267/occurrence-of-pseudocowpox-virus-associated-to-bovine-viral-diarrhea-virus-1-brazilian-amazon
#5
Pedro A Alves, Poliana O Figueiredo, Cairo H S de Oliveira, José D Barbosa, Danillo H S Lima, Henrique A Bomjardim, Natália S Silva, Karinny F Campos, Carlos Magno C Oliveira, Edel Figueiredo Barbosa-Stancioli, Jônatas S Abrahão, Erna G Kroon, Giliane de Souza Trindade
In 2011, an outbreak of severe vesicular disease occurred in the state of Pará, Amazon region. Besides proliferative or verrucous lesions, cattle showed atypical clinical signs such as diarrhea and leading to death. The animals were submitted to clinical, pathological and molecular diagnosis, and laboratory tests have confirmed the presence of Pseudocowpox virus (PCPV), a Parapoxvirus genus member, and have also found Bovine viral diarrhea virus-1 (BVDV-1), probably causing persistent infection. The results of molecular diagnostics, followed by sequencing data demonstrated the circulation of both viruses (PCPV and BVDV-1) in an area previously affected by another poxvirus, as Vaccinia virus...
December 2016: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/27864512/epstein-barr-virus-super-enhancer-ernas-are-essential-for-myc-oncogene-expression-and-lymphoblast-proliferation
#6
Jun Liang, Hufeng Zhou, Catherine Gerdt, Min Tan, Tyler Colson, Kenneth M Kaye, Elliott Kieff, Bo Zhao
Epstein-Barr virus (EBV) super-enhancers (ESEs) are essential for lymphoblastoid cell (LCL) growth and survival. Reanalyses of LCL global run-on sequencing (Gro-seq) data found abundant enhancer RNAs (eRNAs) being transcribed at ESEs. Inactivation of ESE components, EBV nuclear antigen 2 (EBNA2) and bromodomain-containing protein 4 (BRD4), significantly decreased eRNAs at ESEs -428 and -525 kb upstream of the MYC oncogene transcription start site (TSS). shRNA knockdown of the MYC -428 and -525 ESE eRNA caused LCL growth arrest and reduced cell growth...
November 18, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27816392/assessing-pdl-1-and-pd-1-in-non-small-cell-lung-cancer-a-novel-immunoscore-approach
#7
Erna-Elise Paulsen, Thomas K Kilvaer, Mehrdad Rakaee Khanehkenari, Samer Al-Saad, Sigurd M Hald, Sigve Andersen, Elin Richardsen, Nora Ness, Lill-Tove Busund, Roy M Bremnes, Tom Donnem
INTRODUCTION: Immune checkpoint inhibitors targeting programmed cell death protein 1 (PD-1) or its ligand, PD-L1, have gained momentum in the treatment of non-small cell lung cancer (NSCLC). However, their prognostic significance remains controversial. The present study evaluated the expression of PD-L1 and PD-1 and their potential role in an Immunoscore, supplementing the TNM classification of NSCLC. MATERIALS AND METHODS: Tissue microarrays constructed from tumor tissue samples from 2 cohorts of a total of 536 patients (University Hospital of North Norway, n = 285; Nordland Hospital, n = 251) with primary resected stage I to IIIA NSCLC...
October 5, 2016: Clinical Lung Cancer
https://www.readbyqxmd.com/read/27810192/self-reported-exposure-to-traffic-pollution-in-relation-to-daytime-sleepiness-and-habitual-snoring-a-questionnaire-study-in-seven-north-european-cities
#8
Thorarinn Gislason, Randi J Bertelsen, Francisco Gomez Real, Torben Sigsgaard, Karl A Franklin, Eva Lindberg, Christer Janson, Erna Sif Arnardottir, Johan Hellgren, Bryndis Benediktsdottir, Bertil Forsberg, Ane Johannessen
OBJECTIVE/BACKGROUND: Little is known about associations between traffic exposure and sleep disturbances. We examined if self-reported exposure to traffic is associated with habitual snoring and daytime sleepiness in a general population. METHODS: In the RHINE III study, 12184 adults answered questions on sleep disturbances and traffic exposure. We analysed bedrooms near roads with traffic, bedrooms with traffic noise, and travelling regularly along busy roads as proxies for traffic exposures, using logistic regression...
August 2016: Sleep Medicine
https://www.readbyqxmd.com/read/27798016/supporting-the-provision-of-palliative-care-in-the-home-environment-a-proof-of-concept-single-arm-trial-of-a-palliative-carers-education-package-precept
#9
Liz Forbat, Erna Haraldsdottir, Marsha Lewis, Ken Hepburn
INTRODUCTION: Practical educational interventions for palliative carers are needed. Current supports frequently rely on carers travelling to a central venue to receive education. A substantial gap therefore exists around determining how high-quality relevant information can be delivered nationally, with limited cost implications, using educational methods that are acceptable to carers in palliative care. This study seeks to design and assess feasibility and acceptability of a distance-learning approach to educating carers...
October 25, 2016: BMJ Open
https://www.readbyqxmd.com/read/27787634/-the-country-has-new-men-men-reconcile-caregiving-with-profession
#10
Erna C Dosch
BACKGROUND: Due to demographic changes and the associated consequences, men are increasingly being confronted with the challenge to reconcile caregiving and profession. Statistical data confirm that the proportion of male caregivers is continuously increasing. These men operate in a field of specifically female connotation, with which they are not too familiar. The investigation of action strategies of male caregivers has long been neglected in scientific research. OBJECTIVE: This study aimed to provide a differentiated approach to a male caregiving behavior...
October 27, 2016: Zeitschrift Für Gerontologie und Geriatrie
https://www.readbyqxmd.com/read/27771792/molecular-evidence-of-orthopoxvirus-dna-in-capybara-hydrochoerus-hydrochaeris-stool-samples
#11
Lara Ambrosio Leal Dutra, Gabriel Magno de Freitas Almeida, Graziele Pereira Oliveira, Jônatas Santos Abrahão, Erna Geessien Kroon, Giliane de Souza Trindade
Vaccinia virus (VACV) is responsible for outbreaks in Brazil and has immense potential as an emerging virus. VACV can be found naturally circulating in India, Pakistan and South America, where it causes infections characterised by exanthematic lesions in buffaloes, cattle and humans. The transmission cycle of Brazilian VACV has still not been fully characterised; one of the most important gaps in knowledge being the role of wild animals. Capybaras, which are restricted to the Americas, are the world's largest rodents and have peculiar characteristics that make them possible candidates for being part of a natural VACV reservoir...
October 22, 2016: Archives of Virology
https://www.readbyqxmd.com/read/27756313/erratum-to-respiratory-symptoms-sleep-disordered-breathing-and-biomarkers-in-nocturnal-gastroesophageal-reflux
#12
Össur Ingi Emilsson, Bryndís Benediktsdóttir, Ísleifur Ólafsson, Elizabeth Cook, Sigurður Júlíusson, Einar Stefán Björnsson, Sunna Guðlaugsdóttir, Anna Soffía Guðmundsdóttir, Ekaterina Mirgorodskaya, Evert Ljungström, Erna Sif Arnardóttir, Þórarinn Gíslason, Christer Janson, Anna-Carin Olin
No abstract text is available yet for this article.
October 18, 2016: Respiratory Research
https://www.readbyqxmd.com/read/27744138/the-significance-of-personal-learning-environments-ples-in-nursing-education-extending-current-conceptualizations
#13
Christopher Patterson, Moira Stephens, Vico Chiang, Ann M Price, Fiona Work, Erna Snelgrove-Clarke
BACKGROUND: Personal learning environments (PLEs) have been shown to be a critical part of how students negotiate and manage their own learning. Understandings of PLEs appear to be constrained by narrow definitions that focus primarily on technological engagement with a range of web tools and associated applications. This paper addresses a gap in the literature around PLEs for students currently enrolled in undergraduate nursing degrees. PURPOSE: To provide in-depth insights into how undergraduate students of nursing manage and experience their learning...
January 2017: Nurse Education Today
https://www.readbyqxmd.com/read/27708229/prognostic-effect-of-intratumoral-neutrophils-across-histological-subtypes-of-non-small-cell-lung-cancer
#14
Mehrdad Rakaee, Lill-Tove Busund, Erna-Elise Paulsen, Elin Richardsen, Samer Al-Saad, Sigve Andersen, Tom Donnem, Roy M Bremnes, Thomas K Kilvaer
Recent data indicate that tumor-associated neutrophils (TANs) serve a dual role in tumor progression and regression. CD66b is a neutrophil marker and has been associated with patient outcome in various cancers. However, its clinical impact in non-small cell lung cancer (NSCLC) remains controversial. 536 NSCLC patients, of which 172 harbored lymph node metastases, were included in this study. Tissue microarrays were constructed and multiplexed immunohistochemistry of CD66b, CD34 and pan-keratin was performed to evaluate the localization and quantity of CD66b+ TANs...
September 30, 2016: Oncotarget
https://www.readbyqxmd.com/read/27707886/exploitation-of-castration-resistant-prostate-cancer-transcription-factor-dependencies-by-the-novel-bet-inhibitor-abbv-075
#15
Emily J Faivre, Denise Wilcox, Xiaoyu Lin, Paul Hessler, Maricel Torrent, Wei He, Tamar Uziel, Daniel H Albert, Keith McDaniel, Warren Kati, Yu Shen
: Competitive inhibitors of acetyl-lysine binding to the bromodomains of the BET (bromodomain and extra terminal) family are being developed for the treatment of solid and hematologic malignancies. The function of BET family member BRD4 at enhancers/super-enhancers has been shown to sustain signal-dependent or pathogenic gene expression programs. Here the hypothesis was tested that the transcription factor drivers of castration-resistant prostate cancer (CRPC) clinical progression, including the Androgen Receptor (AR), are critically dependent on BRD4 and thus represent a sensitive solid tumor indication for the BET inhibitor ABBV-075...
October 5, 2016: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/27691922/using-peer-mediated-instruction-to-support-communication-involving-a-student-with-autism-during-mathematics-activities-a-case-study
#16
Paulo Tan, Erna Alant
This study employed an A-B singled subject design to explore the extent to which a peer-mediated intervention supported a first-grade student with autism's usage both in purpose and frequency of a speech-generating device (SGD) during mathematics activities. The intervention involved teaching a peer without a disability to encourage the student with autism to use the SGD during partnered mathematics activities. Our analysis involved visual and descriptive examination of trends and patterns over time, and comparison of means between and within phases...
October 3, 2016: Assistive Technology: the Official Journal of RESNA
https://www.readbyqxmd.com/read/27681417/super-enhancers-at-the-nanog-locus-differentially-regulate-neighboring-pluripotency-associated-genes
#17
Steven Blinka, Michael H Reimer, Kirthi Pulakanti, Sridhar Rao
Super-enhancers are tissue-specific cis-regulatory elements that drive expression of genes associated with cell identity and malignancy. A cardinal feature of super-enhancers is that they are transcribed to produce enhancer-derived RNAs (eRNAs). It remains unclear whether super-enhancers robustly activate genes in situ and whether their functions are attributable to eRNAs or the DNA element. CRISPR/Cas9 was used to systematically delete three discrete super-enhancers at the Nanog locus in embryonic stem cells, revealing functional differences in Nanog transcriptional regulation...
September 27, 2016: Cell Reports
https://www.readbyqxmd.com/read/27669904/identification-and-molecular-characterization-of-bacteriophage-phiaxp-2-of-achromobacter-xylosoxidans
#18
Erna Li, Zhe Yin, Yanyan Ma, Huan Li, Weishi Lin, Xiao Wei, Ruixiang Zhao, Aimin Jiang, Jing Yuan, Xiangna Zhao
A novel Achromobacter xylosoxidans bacteriophage, phiAxp-2, was isolated from hospital sewage in China. The phage was morphologically and microbiologically characterized, and its one-step growth curve, host range, genomic sequence, and receptor were determined. Its morphology showed that phiAxp-2 belongs to the family Siphoviridae. Microbiological characterization demonstrated that pH 7 is most suitable for phage phiAxp-2; its titer decreased when the temperature exceeded 50 °C; phiAxp-2 is sensitive to ethanol and isopropanol; and the presence of calcium and magnesium ions is necessary to accelerate cell lysis and improve the formation of phiAxp-2 plaques...
September 27, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27662874/computational-approaches-for-mining-gro-seq-data-to-identify-and-characterize-active-enhancers
#19
Anusha Nagari, Shino Murakami, Venkat S Malladi, W Lee Kraus
Transcriptional enhancers are DNA regulatory elements that are bound by transcription factors and act to positively regulate the expression of nearby or distally located target genes. Enhancers have many features that have been discovered using genomic analyses. Recent studies have shown that active enhancers recruit RNA polymerase II (Pol II) and are transcribed, producing enhancer RNAs (eRNAs). GRO-seq, a method for identifying the location and orientation of all actively transcribing RNA polymerases across the genome, is a powerful approach for monitoring nascent enhancer transcription...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27662872/identification-of-transcribed-enhancers-by-genome-wide-chromatin-immunoprecipitation-sequencing
#20
Steven Blinka, Michael H Reimer, Kirthi Pulakanti, Luca Pinello, Guo-Cheng Yuan, Sridhar Rao
Recent work has shown that RNA polymerase II-mediated transcription at distal cis-regulatory elements serves as a mark of highly active enhancers. Production of noncoding RNAs at enhancers, termed eRNAs, correlates with higher expression of genes that the enhancer interacts with; hence, eRNAs provide a new tool to model gene activity in normal and disease tissues. Moreover, this unique class of noncoding RNA has diverse roles in transcriptional regulation. Transcribed enhancers can be identified by a common signature of epigenetic marks by overlaying a series of genome-wide chromatin immunoprecipitation and RNA sequencing datasets...
2017: Methods in Molecular Biology
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