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macrophage extracellular vesicles proteomics

Majid Alhomrani, Jeanne Correia, Marcus Zavou, Bryan Leaw, Nathan Kuk, Rong Xu, Mohamed I Saad, Alexander Hodge, David W Greening, Rebecca Lim, William Sievert
Background: Hepatic stellate cells (HSCs) are the primary collagen-secreting cells in the liver. While HSCs are the major cell type involved in the pathogenesis of liver fibrosis, hepatic macrophages also play an important role in mediating fibrogenesis and fibrosis resolution. Previously, we observed a reduction in HSC activation, proliferation, and collagen synthesis following exposure to human amnion epithelial cells (hAEC) and hAEC-conditioned media (hAEC-CM). This suggested that specific factors secreted by hAEC might be effective in ameliorating liver fibrosis...
2017: Frontiers in Pharmacology
Yvonne Couch, Naveed Akbar, Simon Davis, Roman Fischer, Alex M Dickens, Ain A Neuhaus, Annette I Burgess, Peter M Rothwell, Alastair M Buchan
BACKGROUND AND PURPOSE: Extracellular vesicles (EVs) are protein-lipid complexes released from cells, as well as actively exocytosed, as part of normal physiology, but also during pathological processes such as those occurring during a stroke. Our aim was to determine the inflammatory potential of stroke EVs. METHODS: EVs were quantified and analyzed in the sera of patients after an acute stroke (<24 hours; OXVASC [Oxford Vascular Study]). Isolated EV fractions were subjected to untargeted proteomic analysis by liquid chromatography mass-spectrometry/mass-spectrometry and then applied to macrophages in culture to investigate inflammatory gene expression...
August 2017: Stroke; a Journal of Cerebral Circulation
Martina B Lorey, Katriina Rossi, Kari K Eklund, Tuula A Nyman, Sampsa Matikainen
Gram-negative bacteria are associated with a wide spectrum of infectious diseases in humans. Inflammasomes are cytosolic protein complexes that are assembled when the cell encounters pathogens or other harmful agents. The non-canonical caspase-4/5 inflammasome is activated by Gram-negative bacteria-derived lipopolysaccharide (LPS) and by endogenous oxidized phospholipids. Protein secretion is a critical component of the innate immune response. Here, we have used label-free quantitative proteomics to characterize global protein secretion in response to non-canonical inflammasome activation upon intracellular LPS recognition in human primary macrophages...
April 2017: Molecular & Cellular Proteomics: MCP
Chenguang Niu, Xu Wang, Mingming Zhao, Tanxi Cai, Peibin Liu, Jizhao Li, Belinda Willard, Lingyun Zu, Enchen Zhou, Yufeng Li, Bing Pan, Fuquan Yang, Lemin Zheng
BACKGROUND: A new mechanism for intercellular communication has recently emerged that involves intercellular transfer of extracellular vesicles (EVs). Several studies have indicated that EVs may play a potential role in cell-to-cell communication between macrophage foam cells and vascular smooth muscle cells (VSMCs) in atherosclerotic lesion. METHODS AND RESULTS: This study involved the comparison of circulating EVs from atherosclerotic patients and control participants...
October 17, 2016: Journal of the American Heart Association
Thiago Aparecido da Silva, Maria Cristina Roque-Barreira, Arturo Casadevall, Fausto Almeida
Extracellular vesicles (EVs) released by eukaryotes, archaea, and bacteria contain proteins, lipids, polysaccharides, and other molecules. The cargo analysis of EVs shows that they contain virulence factors suggesting a role in the pathogenesis of infection. The proteome, lipidome, RNA content, and carbohydrate composition of EVs from Paracoccidioides brasiliensis and Paracoccidioides lutzii were characterized. However, the effects of P. brasiliensis EVs on the host immune system have not yet been investigated...
October 24, 2016: Scientific Reports
Jose Antonio Reales-Calderón, Catarina Vaz, Lucía Monteoliva, Gloria Molero, Concha Gil
The effectiveness of macrophages in the response to systemic candidiasis is crucial to an effective clearance of the pathogen. The secretion of proteins, mRNAs, noncoding RNAs and lipids through extracellular vesicles (EVs) is one of the mechanisms of communication between immune cells. EVs change their cargo to mediate different responses, and may play a role in the response against infections. Thus we have undertaken the first quantitative proteomic analysis on the protein composition of THP-1 macrophage-derived EVs during the interaction with Candida albicans...
January 6, 2017: Journal of Proteome Research
Wojciech Cypryk, Martina Lorey, Anne Puustinen, Tuula A Nyman, Sampsa Matikainen
Influenza A viruses (IAVs) are aggressive pathogens that cause acute respiratory diseases and annual epidemics in humans. Host defense against IAV infection is initiated by macrophages, which are the principal effector cells of the innate immune system. We have previously shown that IAV infection of human macrophages is associated with robust secretion of proteins via conventional and unconventional protein release pathways. Here we have characterized unconventional, extracellular vesicle (EV)-mediated protein secretion in human macrophages during IAV infection using proteomics, bioinformatics, and functional studies...
January 6, 2017: Journal of Proteome Research
Dionysios C Watson, Defne Bayik, Avinash Srivatsan, Cristina Bergamaschi, Antonio Valentin, Gang Niu, Jenifer Bear, Mitchell Monninger, Mei Sun, Aizea Morales-Kastresana, Jennifer C Jones, Barbara K Felber, Xiaoyuan Chen, Ihsan Gursel, George N Pavlakis
Extracellular vesicles (EV), including exosomes and microvesicles, are nano-sized intercellular communication vehicles that participate in a multitude of physiological processes. Due to their biological properties, they are also promising candidates for the systemic delivery of therapeutic compounds, such as cytokines, chemotherapeutic drugs, siRNAs and viral vectors. However, low EV production yield and rapid clearance of administered EV by liver macrophages limit their potential use as therapeutic vehicles...
October 2016: Biomaterials
Yinghui Zhu, Xianwei Chen, Qingfei Pan, Yang Wang, Siyuan Su, Cuicui Jiang, Yang Li, Ningzhi Xu, Lin Wu, Xiaomin Lou, Siqi Liu
Exosomes are 30-120 nm-sized membrane vesicles of endocytic origin that are released into the extracellular environment and play roles in cell-cell communication. Tumor-associated macrophages (TAMs) are important constituents of the tumor microenvironment; thus, it is critical to study the features and complex biological functions of TAM-derived exosomes. Here, we constructed a TAM cell model from a mouse macrophage cell line, Ana-1, and performed comparative proteomics on exosomes, exosome-free media, and cells between TAMs and Ana-1...
October 2, 2015: Journal of Proteome Research
Radu Albulescu, Cristiana Tanase, Elena Codrici, Daniela I Popescu, Sanda M Cretoiu, Laurentiu M Popescu
Telocytes (TCs) are interstitial cells that are present in numerous organs, including the heart interstitial space and cardiac stem cell niche. TCs are completely different from fibroblasts. TCs release extracellular vesicles that may interact with cardiac stem cells (CSCs) via paracrine effects. Data on the secretory profile of TCs and the bidirectional shuttle vesicular signalling mechanism between TCs and CSCs are scarce. We aimed to characterize and understand the in vitro effect of the TC secretome on CSC fate...
August 2015: Journal of Cellular and Molecular Medicine
Bruno Haas, Daniel Grenier
Streptococcus suis, more particularly serotype 2, is a major swine pathogen and an emerging zoonotic agent worldwide that mainly causes meningitis, septicemia, endocarditis, and pneumonia. Although several potential virulence factors produced by S. suis have been identified in the last decade, the pathogenesis of S. suis infections is still not fully understood. In the present study, we showed that S. suis produces membrane vesicles (MVs) that range in diameter from 13 to 130 nm and that appear to be coated by capsular material...
2015: PloS One
Julie M Wolf, Javier Espadas, Jose Luque-Garcia, Todd Reynolds, Arturo Casadevall
Microbial secretion is integral for regulating cell homeostasis as well as releasing virulence factors during infection. The genes encoding phosphatidylserine synthase (CHO1) and phosphatidylserine decarboxylase (PSD1 and PSD2) are Candida albicans genes involved in phospholipid biosynthesis, and mutations in these genes affect mitochondrial function, cell wall thickness, and virulence in mice. We tested the roles of these genes in several agar-based secretion assays and observed that the cho1Δ/Δ and psd1Δ/Δ psd2Δ/Δ strains manifested less protease and phospholipase activity...
August 2015: Eukaryotic Cell
Jeroen de Vrij, S L Niek Maas, Kitty M C Kwappenberg, Rosalie Schnoor, Anne Kleijn, Lennard Dekker, Theo M Luider, Lot D de Witte, Manja Litjens, Miriam E van Strien, Elly M Hol, Jerome Kroonen, Pierre A Robe, Martine L Lamfers, Marco W Schilham, Marike L D Broekman
Glioblastoma multiforme (GBM) is the most common primary brain tumor and is without exception lethal. GBMs modify the immune system, which contributes to the aggressive nature of the disease. Particularly, cells of the monocytic lineage, including monocytes, macrophages and microglia, are affected. We investigated the influence of GBM-derived extracellular vesicles (EVs) on the phenotype of monocytic cells. Proteomic profiling showed GBM EVs to be enriched with proteins functioning in extracellular matrix interaction and leukocyte migration...
October 1, 2015: International Journal of Cancer. Journal International du Cancer
Gabriele Vargas, Juliana D B Rocha, Debora Leite Oliveira, Priscila Costa Albuquerque, Susana Frases, Suelen S Santos, Joshua Daniel Nosanchuk, Andre Marco Oliveira Gomes, Lia C A S Medeiros, Kildare Miranda, Tiago J P Sobreira, Ernesto S Nakayasu, Emma A Arigi, Arturo Casadevall, Allan J Guimaraes, Marcio L Rodrigues, Celio Geraldo Freire-de-Lima, Igor C Almeida, Leonardo Nimrichter
The release of extracellular vesicles (EV) by fungal organisms is considered an alternative transport mechanism to trans-cell wall passage of macromolecules. Previous studies have revealed the presence of EV in culture supernatants from fungal pathogens, such as Cryptococcus neoformans, Histoplasma capsulatum, Paracoccidioides brasiliensis, Sporothrix schenckii, Malassezia sympodialis and Candida albicans. Here we investigated the size, composition, kinetics of internalization by bone marrow-derived murine macrophages (MO) and dendritic cells (DC), and the immunomodulatory activity of C...
March 2015: Cellular Microbiology
Rocío García-Rodas, Radames J B Cordero, Nuria Trevijano-Contador, Guilhem Janbon, Frédérique Moyrand, Arturo Casadevall, Oscar Zaragoza
UNLABELLED: The fungal pathogen Cryptococcus neoformans has several virulence factors, among which the most important is a polysaccharide capsule. The size of the capsule is variable and can increase significantly during infection. In this work, we investigated the relationship between capsular enlargement and the cell cycle. Capsule growth occurred primarily during the G1 phase. Real-time visualization of capsule growth demonstrated that this process occurred before the appearance of the bud and that capsule growth arrested during budding...
2014: MBio
Wojciech Cypryk, Tiina Ohman, Eeva-Liisa Eskelinen, Sampsa Matikainen, Tuula A Nyman
Fungal infections (mycoses) are common diseases of varying severity that cause problems, especially to immunologically compromised people. Fungi express a variety of pathogen-associated molecular patterns on their surface including β-glucans, which are important immunostimulatory components of fungal cell walls. During stimulatory conditions of infection and colonization, besides intensive intracellular response, human cells actively communicate on the intercellular level by secreting proteins and other biomolecules with several mechanisms...
May 2, 2014: Journal of Proteome Research
Juho J Miettinen, Sampsa Matikainen, Tuula A Nyman
Herpes simplex virus 1 (HSV-1) is a common pathogen infecting the majority of people worldwide at some stage in their lives. The early host response to viral infection is initiated by the cells of the innate immune response, including macrophages. Here, we have characterized the secretome of HSV-1-infected human primary macrophages using high-throughput quantitative proteomics. We identified and quantified 516 distinct human proteins with high confidence from the macrophage secretome upon HSV-1 infection, and the secretion of 411 proteins was >2-fold increased upon beta interferon (IFN-β) priming and/or HSV-1 infection...
December 2012: Journal of Virology
Jose Antonio Mendez, Nelson C Soares, Jesús Mateos, Carmen Gayoso, Carlos Rumbo, Jesús Aranda, Maria Tomas, Germán Bou
The study of the extracellular proteomes of pathogenic bacteria is essential for gaining insights into the mechanisms of pathogenesis and for the identification of virulence factors. Through the use of different proteomic approaches, namely Nano-LC and 2DE combined with MALDI-TOF/TOF, we have characterized the extracellular proteome of a highly invasive, multidrug-resistant strain of A. baumannii (clone AbH12O-A2). This study focused on two main protein fractions of the extracellular proteome: proteins that are exported by outer membrane vesicles (OMVs) and freely soluble extracellular proteins (FSEPs) present in the culture medium of A...
December 7, 2012: Journal of Proteome Research
Neha Biyani, Rentala Madhubala
Protozoan parasites of the genus Leishmania are important human pathogens that cycle between an extracellular promastigote stage residing in the sandflies and an intracellular amastigote stage colonizing the phagolysosomal compartment of the mammalian macrophages. Here, we used the isobaric tagging method to quantify the global proteomic differences between the promastigotes and the intracellular amastigotes of three different Leishmania donovani clones derived from the THP-1 human macrophage cell line. We identified a substantial number of differentially modulated proteins involved in nutrient acquisition and energy metabolism, cell motility and cytoskeleton, transport, cell signaling and stress response...
December 2012: Biochimica et Biophysica Acta
Tony Pierson, Demetrios Matrakas, Yuka U Taylor, Ganiraju Manyam, Victor N Morozov, Weidong Zhou, Monique L van Hoek
We have isolated and characterized outer membrane vesicles (OMVs) from Francisella. Transport of effector molecules through secretion systems is a major mechanism by which Francisella tularensis alters the extracellular proteome and interacts with the host during infection. Outer membrane vesicles produced by Francisella were examined using TEM and AFM and found to be 43-125 nm in size, representing another potential mechanism for altering the extracellular environment. A proteomic analysis (LC-MS/MS) of OMVs from F...
March 4, 2011: Journal of Proteome Research
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