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https://www.readbyqxmd.com/read/28717416/feasibility-of-urinary-extracellular-vesicle-proteome-profiling-using-a-robust-and-simple-clinically-applicable-isolation-method
#1
Irene V Bijnsdorp, Olga Maxouri, Aarzo Kardar, Tim Schelfhorst, Sander R Piersma, Thang V Pham, Andre Vis, R Jeroen van Moorselaar, Connie R Jimenez
Extracellular vesicles (EVs) secreted by prostate cancer (PCa) cells contain specific biomarkers and can be isolated from urine. Collection of urine is not invasive, and therefore urinary EVs represent a liquid biopsy for diagnostic and prognostic testing for PCa. In this study, we optimised urinary EV isolation using a method based on heat shock proteins and compared it to gold-standard ultracentrifugation. The urinary EV isolation protocol using the Vn96-peptide is easier, time convenient (≈1.5 h) and no special equipment is needed, in contrast to ultracentrifugation protocol (>3...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28714175/schwann-cells-secrete-extracellular-vesicles-to-promote-and-maintain-the-proliferation-and-multipotency-of-hdpcs
#2
Ziyue Li, Yan Liang, Kuangwu Pan, Hui Li, Mei Yu, Weihua Guo, Guoqing Chen, Weidong Tian
OBJECTIVES: Schwann cells (SCs) are the principal glial cells in peripheral nerve system, involved in neuropathies with great regenerative potential. Dental pulp cells have been reported to maintain neurogenic potential. In contrast, the regulatory role of SCs on human dental pulp cells (hDPCs) development remains undefined. MATERIALS AND METHODS: SC secretion and SC-derived extracellular vesicles (EVs) were collected and used to treat hDPCs; and proliferation and multiple differentiation of hDPCs were detected after EVs treatments...
August 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28687758/directional-exosome-proteomes-reflect-polarity-specific-functions-in-retinal-pigmented-epithelium-monolayers
#3
Mikael Klingeborn, W Michael Dismuke, Nikolai P Skiba, Una Kelly, W Daniel Stamer, Catherine Bowes Rickman
The retinal pigmented epithelium (RPE) forms the outer blood-retinal barrier in the eye and its polarity is responsible for directional secretion and uptake of proteins, lipoprotein particles and extracellular vesicles (EVs). Such a secretional division dictates directed interactions between the systemic circulation (basolateral) and the retina (apical). Our goal is to define the polarized proteomes and physical characteristics of EVs released from the RPE. Primary cultures of porcine RPE cells were differentiated into polarized RPE monolayers on permeable supports...
July 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28679310/protein-and-molecular-characterisation-of-a-clinically-compliant-amniotic-fluid-stem-cell-derived-extracellular-vesicle-fraction-capable-of-accelerating-muscle-regeneration-through-the-enhancement-of-angiogenesis
#4
Ben Mellows, Robert Mitchell, Manuela Antonioli, Oliver Kretz, David Chamber, Marie-Theres Zeuner, Bernd Denecke, Luca Musante, Durrgah Ramachandra, Florence Debacq-Chainiaux, Harry Holthofer, Barbara Joch, Steve Ray, Darius Widera, Anna L David, Tobios B Huber, Joern Dengjel, Paolo De Coppi, Ketan Patel
The secretome of human amniotic fluid stem cells (AFSC) has great potential as a therapeutic agent in regenerative medicine. However it must be produced in a clinically compliant manner before it can be used in humans. Here we developed a means of producing a biologically active secretome from AFSCs that is free of all exogenous molecules. We demonstrate that the full secretome is capable of promoting stem cell proliferation, migration and protection of cells against senescence. Furthermore, it has significant anti-inflammatory properties...
July 5, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28674889/proteomics-analysis-of-circulating-serum-exosomes
#5
Antonius Koller, Purvi Patel, Jenny Kim Kim, Emily I Chen
Proteomics characterization of biofluids, such as urine and plasma, has been explored for the discovery of predictive, prognostic, and mechanistic biomarkers of diseases and tissue injury. Here we describe comprehensive characterization of protein cargos from cell-derived secreted vesicles (extracellular vesicles or exosome) for biomarker discovery using the mass spectrometry-based technology.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28674887/simultaneous-enrichment-of-plasma-extracellular-vesicles-and-glycoproteome-for-studying-disease-biomarkers
#6
Sunil S Adav, Siu Kwan Sze
To detect disease at an early stage and to develop effective disease treatment therapies, reliable biomarkers of diagnosis, disease progression, and its status remain a research priority. A majority of disease pathologies are primarily associated with different subsets of cells of different tissues, discrete compartments, and areas. These subsets of cells release glycoproteins and specific extracellular vesicles (EVs) including microvesicles and exosomes that carry bioactive cargoes of proteins, nucleic acids, and metabolites...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28674884/targeted-approach-for-proteomic-analysis-of-a-hidden-membrane-protein
#7
Tania Martins-Marques, Sandra I Anjo, Teresa Ribeiro-Rodrigues, Bruno Manadas, Henrique Girao
Given the properties of plasma membrane proteins, namely, their hydrophobicity, low solubility, and high resistance to digestion and extraction, their identification by traditional mass spectrometry (MS) has been a challenging task. Hence, proteomic studies involving the transmembrane protein connexin43 (Cx43) are scarce. Additionally, studies demonstrating the presence of proteins embedded in the lipid bilayer of extracellular vesicles (EVs) are difficult to perform and require specific changes and fine adjustments in the experimental and technical procedure to allow their detection by MS...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28674883/proteomic-analysis-of-blood-extracellular-vesicles-in-cardiovascular-disease-by-lc-ms-ms-analysis
#8
Montserrat Baldan-Martin, Fernando de la Cuesta, Gloria Alvarez-Llamas, Gema Ruiz-Hurtado, Luis M Ruilope, Maria G Barderas
Extracellular vesicles are membrane vesicles related to cell communication. These vesicles consist of proteins, RNA, and microRNA and are an interesting and important tool to understand the processes taking place in the secreting cell, especially in diseases in which its release is often enhanced. The used of blood extracellular vesicles in cardiovascular disease as a low invasive, easily accessible source of circulating markers could give us important information related to pathological process even more with the use of proteomic analysis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28669749/extracellular-vesicles-in-gastrointestinal-cancer-in-conjunction-with-microbiota-on-the-border-of-kingdoms
#9
REVIEW
Natasha S Barteneva, Yeldar Baiken, Elizaveta Fasler-Kan, Kenneth Alibek, Sheng Wang, Natalia Maltsev, Eugeny D Ponomarev, Zarina Sautbayeva, Sholpan Kauanova, Anna Moore, Christoph Beglinger, Ivan A Vorobjev
Extracellular vesicle (EV) production is a universal feature of metazoan cells as well as prokaryotes (bMVs - bacterial microvesicls). They are small vesicles with phospholipid membrane carrying proteins, DNA and different classes of RNAs and are heavily involved in intercellular communication acting as vectors of information to target cells. For the last decade, the interest in EV research has exponentially increased though thorough studies of their roles in various pathologies that was not previously possible due to technical limitations...
June 29, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28660234/isolation-of-exosomes-from-semen-for-in-vitro-uptake-and-hiv-1-infection-assays
#10
Marisa N Madison, Jennifer L Welch, Chioma M Okeoma
Exosomes are membranous extracellular nanovesicles of endocytic origin. Exosomes are known to carry host and pathogen-derived genomic, proteomic, lipidomic cargos and other extraneous molecules. Exosomes are secreted by diverse cell types into the extracellular milieu and are subsequently internalized by recipient neighboring or distal cells. Upon internalization, exosomes condition recipient cells by donating their cargos and/or activating various signal transduction pathways, consequently regulating physiological and pathophysiological processes...
April 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28643940/proteomic-profiling-of-extracellular-vesicles-secreted-from-toxoplasma-gondii
#11
Pryscilla Fanini Wowk, Maria Luisa Zardo, Halisson Tesseroli Miot, Samuel Goldenberg, Paulo Costa Carvalho, Patricia Alves Mörking
Toxoplasma gondii infects a wide range of hosts worldwide, including humans and domesticated animals causing toxoplasmosis disease. Recently, exosomes, small extracellular vesicles (EV) that contain nucleic acids, proteins, and lipids derived from their original cells were linked with disease protection. The effect of EVs derived from T. gondii on the immune response and its relevance in a physiological context is unknown. Here we disclose the first proteomic profiling of T. gondii EVs compared to EVs isolated from a human foreskin fibroblast infected cell line cultured in a vesicle- free medium...
June 23, 2017: Proteomics
https://www.readbyqxmd.com/read/28618037/hydrogen-peroxide-stimulates-exosomal-cathepsin-b-regulation-of-the-receptor-for-advanced-glycation-end-products-rage
#12
Charles A Downs, Viet D Dang, Nicholle M Johnson, Nancy Denslow, Abdel A Alli
Exosomes are nano-sized vesicles that are secreted into the extracellular environment. These vesicles contain various biological effector molecules that can regulate intracellular signaling pathways in recipient cells. The aim of this study was to examine a correlation between exosomal cathepsin B activity and the receptor for advanced glycation end-products (RAGE). Type 1 alveolar epithelial (R3/1) cells were treated with or without hydrogen peroxide and exosomes isolated from the cell conditioned media were characterized by NanoSight analysis...
June 15, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28606449/transfusion-related-immunomodulation-and-cancer
#13
REVIEW
Hadi Goubran, David Sheridan, Julia Radosevic, Thierry Burnouf, Jerard Seghatchian
Blood and blood-component therapy triggers immunological reactions in recipients. Transfusion-related immunomodulation [TRIM] is an important complex biological immune reaction to transfusion culminating in immunosuppression. The mechanisms underlying TRIM include the presence of residual leukocytes and apoptotic cells, the transfusion of immunosuppressive cytokines either present in donor components or generated during blood processing, the transfer of metabolically active growth factor-loaded microparticles and extracellular vesicles and the presence of free hemoglobin or extracellular vesicle-bound hemoglobin...
May 27, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28596424/s100-a9-protein-in-exosomes-from-chronic-lymphocytic-leukemia-cells-promotes-nf-%C3%AE%C2%BAb-activity-during-disease-progression
#14
Daniel Prieto, Natalia Sotelo, Noé Seija, Sandra Sernbo, Cecilia Abreu, Rosario Durán, Magdalena Gil, Estefanía Sicco, Victoria Irigoin, Carolina Oliver, Ana Inés Landoni, Raúl Gabus, Guillermo Dighiero, Pablo Oppezzo
Chronic Lymphocytic Leukemia (CLL) is an incurable disease characterized by accumulation of clonal B lymphocytes, resulting from a complex balance between cell proliferation and apoptotic death. A continuous crosstalk between cancer cells and local/distant host environment is required for effective tumor growth. Among the main actors of this dynamic interplay between tumoral cells and their microenvironment are the nano-sized vesicles called exosomes. Emerging evidence indicates that secretion, composition, and functional capacity of the exosomes are altered as tumors progress to an aggressive phenotype...
June 8, 2017: Blood
https://www.readbyqxmd.com/read/28594924/multiple-extracellular-vesicle-types-in-peritoneal-dialysis-effluent-are-prominent-and-contain-known-biomarkers
#15
Lachlan James Pearson, I-Yanut Klaharn, Bussakorn Thongsawang, Wasin Manuprasert, Thunvarat Saejew, Poorichaya Somparn, Piyatida Chuengsaman, Talerngsak Kanjanabuch, Trairak Pisitkun
Peritoneal dialysis inevitability results in activation of inflammatory processes and its efficiency is highly variable between patients. An improved method to isolate biomarkers and study pathophysiological mechanisms in peritoneal dialysis effluent (PDE) is expected to be of much benefit for the development of this treatment approach and help with patient management. Extracellular vesicles (EVs) are released as part of normal cellular processes. Their proteome is expected to reflect both type and health of their cell of origin...
2017: PloS One
https://www.readbyqxmd.com/read/28555885/serum-extracellular-vesicles-contain-protein-biomarkers-for-primary-sclerosing-cholangitis-and-cholangiocarcinoma
#16
Ander Arbelaiz, Mikel Azkargorta, Marcin Krawczyk, Alvaro Santos-Laso, Ainhoa Lapitz, Maria J Perugorria, Oihane Erice, Esperanza Gonzalez, Raul Jimenez-Agüero, Adelaida Lacasta, Cesar Ibarra, Alberto Sanchez-Campos, Juan P Jimeno, Frank Lammert, Piotr Milkiewicz, Marco Marzioni, Rocio I R Macias, Jose J G Marin, Tushar Patel, Gregory J Gores, Ibon Martinez, Félix Elortza, Juan M Falcon-Perez, Luis Bujanda, Jesus M Banales
Cholangiocarcinoma (CCA) includes a heterogeneous group of biliary cancers with poor prognosis. Several conditions such as primary sclerosing cholangitis (PSC) are risk factors. Non-invasive differential diagnosis between intrahepatic CCA (iCCA) and hepatocellular carcinoma (HCC) is sometimes difficult. Accurate non-invasive biomarkers for PSC, CCA or HCC are not available. In the search of novel biomarkers, serum extracellular vesicles (EV) were isolated from CCA (n=43), PSC (n=30) or HCC (n=29) patients, and healthy individuals (control, n=32), and their protein content was characterized...
May 26, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28536169/inflammatory-stroke-extracellular-vesicles-induce-macrophage-activation
#17
Yvonne Couch, Naveed Akbar, Simon Davis, Roman Fischer, Alex M Dickens, Ain A Neuhaus, Annette I Burgess, Peter M Rothwell, Alastair M Buchan
BACKGROUND AND PURPOSE: Extracellular vesicles (EVs) are protein-lipid complexes released from cells, as well as actively exocytosed, as part of normal physiology, but also during pathological processes such as those occurring during a stroke. Our aim was to determine the inflammatory potential of stroke EVs. METHODS: EVs were quantified and analyzed in the sera of patients after an acute stroke (<24 hours; OXVASC [Oxford Vascular Study]). Isolated EV fractions were subjected to untargeted proteomic analysis by liquid chromatography mass-spectrometry/mass-spectrometry and then applied to macrophages in culture to investigate inflammatory gene expression...
May 23, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28508366/isolation-and-proteomic-analysis-of-microvesicles-and-exosomes-from-ht22-cells-and-primary-neurons
#18
Richard Witas, Dale Chaput, Hirah Khan, Stanley M Stevens, David Kang
Exosomes and microvesicles are extracellular vesicles (EVs) released by most cell types. The role of EVs as a method of intercellular communication has led to these vesicles becoming a major area of interest in a variety of scientific fields including neuroscience. Emerging evidence is now demonstrating that the biomolecular composition of EVs, especially exosomes, can play a role in the progression of disease including various neurodegenerative diseases and cancer. In addition to the miRNA profiles of EVs, these vesicles also show interesting changes in protein expression profiles under different physiological and pathological conditions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28490497/protein-composition-of-the-hepatitis-a-virus-quasi-envelope
#19
Kevin L McKnight, Ling Xie, Olga González-López, Efraín E Rivera-Serrano, Xian Chen, Stanley M Lemon
The Picornaviridae are a diverse family of RNA viruses including many pathogens of medical and veterinary importance. Classically considered "nonenveloped," recent studies show that some picornaviruses, notably hepatitis A virus (HAV; genus Hepatovirus) and some members of the Enterovirus genus, are released from cells nonlytically in membranous vesicles. To better understand the biogenesis of quasi-enveloped HAV (eHAV) virions, we conducted a quantitative proteomics analysis of eHAV purified from cell-culture supernatant fluids by isopycnic ultracentrifugation...
June 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28489901/characterization-and-proteomic-profile-of-extracellular-vesicles-from-peritoneal-dialysis-efflux
#20
Laura Carreras-Planella, Jordi Soler-Majoral, Cristina Rubio-Esteve, Sara Inés Lozano-Ramos, Marcella Franquesa, Josep Bonet, Maria Isabel Troya-Saborido, Francesc Enric Borràs
Peritoneal Dialysis (PD) is considered the best option for a cost-effective mid-term dialysis in patients with Chronic Renal Failure. However, functional failure of the peritoneal membrane (PM) force many patients to stop PD treatment and start haemodialysis. Currently, PM functionality is monitored by the peritoneal equilibration test, a tedious technique that often show changes when the membrane damage is advanced. As in other pathologies, the identification and characterization of extracellular vesicles (EVs) in the peritoneal dialysis efflux (PDE) may represent a non-invasive alternative to identify biomarkers of membrane failure...
2017: PloS One
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