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https://www.readbyqxmd.com/read/29349926/extracellular-vesicles-from-early-stage-p-falciparum-infected-red-blood-cells-contain-pfemp1-and-induce-transcriptional-changes-in-human-monocytes
#1
Natália G Sampaio, Samantha Emery, Alexandra Garnham, Qiao Y Tan, Xavier Sisquella, Matthew A Pimentel, Neta Regev-Rudzki, Louis Schofield, Emily M Eriksson
Pathogens can release extracellular vesicles (EVs) for cell-cell communication and host modulation. EVs from Plasmodium falciparum, the deadliest malaria parasite species, can transfer drug resistance genes between parasites. EVs from late-stage parasite-infected RBC (iRBC-EVs) are immunostimulatory and affect endothelial cell permeability, but little is known about EVs from early-stage iRBC. We detected the parasite virulence factor PfEMP1, which is responsible for iRBC adherence and a major contributor to disease severity, in EVs only up to 12 hours-post RBC invasion...
January 18, 2018: Cellular Microbiology
https://www.readbyqxmd.com/read/29320704/extracellular-vesicles-provide-a-means-for-tissue-crosstalk-during-exercise
#2
Martin Whitham, Benjamin L Parker, Martin Friedrichsen, Janne R Hingst, Marit Hjorth, William E Hughes, Casey L Egan, Lena Cron, Kevin I Watt, Rhiannon P Kuchel, Navind Jayasooriah, Emma Estevez, Tim Petzold, Catherine M Suter, Paul Gregorevic, Bente Kiens, Erik A Richter, David E James, Jørgen F P Wojtaszewski, Mark A Febbraio
Exercise stimulates the release of molecules into the circulation, supporting the concept that inter-tissue signaling proteins are important mediators of adaptations to exercise. Recognizing that many circulating proteins are packaged in extracellular vesicles (EVs), we employed quantitative proteomic techniques to characterize the exercise-induced secretion of EV-contained proteins. Following a 1-hr bout of cycling exercise in healthy humans, we observed an increase in the circulation of over 300 proteins, with a notable enrichment of several classes of proteins that compose exosomes and small vesicles...
January 9, 2018: Cell Metabolism
https://www.readbyqxmd.com/read/29286309/lowering-low-density-lipoprotein-particles-in-plasma-using-dextran-sulphate-co-precipitates-procoagulant-extracellular-vesicles
#3
Jiong-Wei Wang, Ya-Nan Zhang, Siu Kwan Sze, Sander M van de Weg, Flora Vernooij, Arjan H Schoneveld, Sock-Hwee Tan, Henri H Versteeg, Leo Timmers, Carolyn S P Lam, Dominique P V de Kleijn
Plasma extracellular vesicles (EVs) are lipid membrane vesicles involved in several biological processes including coagulation. Both coagulation and lipid metabolism are strongly associated with cardiovascular events. Lowering very-low- and low-density lipoprotein ((V)LDL) particles via dextran sulphate LDL apheresis also removes coagulation proteins. It remains unknown, however, how coagulation proteins are removed in apheresis. We hypothesize that plasma EVs that contain high levels of coagulation proteins are concomitantly removed with (V)LDL particles by dextran sulphate apheresis...
December 29, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29280568/cancer-associated-fibroblasts-the-architects-of-stroma-remodelling
#4
REVIEW
Alice Santi, Fernanda G Kugeratski, Sara Zanivan
Fibroblasts have exceptional phenotypic plasticity and capability to secrete vast amount of soluble factors, ECM components and extracellular vesicles. While in physiological conditions this makes fibroblasts master regulators of tissue homeostasis and healing of injured tissues, in solid tumours cancer-associated fibroblasts (CAFs) co-evolve with the disease, and alter the biochemical and physical structure of the tumour microenvironment, as well as the behaviour of the surrounding stromal and cancer cells...
December 27, 2017: Proteomics
https://www.readbyqxmd.com/read/29245944/extracellular-vesicles-from-kshv-infected-endothelial-cells-activate-the-complement-system
#5
Hyungtaek Jeon, Seung-Min Yoo, Hyo Sun Choi, Ji Young Mun, Hee-Gyoo Kang, Jiyeong Lee, Jinsung Park, Shou-Jiang Gao, Myung-Shin Lee
Extracellular vesicles (EVs), released by cells, are associated with cell-to-cell communication and regulate various cellular processes. EVs draw parallels with viruses for their similar structures and functions. Increasing evidences from recent studies indicate that cells infected with viruses release a variety of EVs. Delineating the functions and mechanisms of EVs released during virus infection is essential for understanding the molecular basis of viral infection and replication as well as associated pathogenesis...
November 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/29228589/comparison-of-echinococcus-multilocularis-and-echinococcus-granulosus-hydatid-fluid-proteome-provides-molecular-strategies-for-specialized-host-parasite-interactions
#6
Chun-Seob Ahn, Jeong-Geun Kim, Xiumin Han, Insug Kang, Yoon Kong
Alveolar and cystic echinococcoses, caused by the metacestodes of Echinococcus multilocularis and E. granulosus, are prevalent in several regions and invoke deleterious zoonotic helminthiases. Hydatid fluid (HF), which contains proteinaceous and non-proteinaceous secretions of the parasite- and host-derived components, critically affects the host-parasite interplay and disease progression. We conducted HF proteome profiling of fully mature E. multilocularis vesicle (nine months postinfection) and E. granulosus cyst (stage 2)...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29228011/characterization-of-the-giardia-intestinalis-secretome-during-interaction-with-human-intestinal-epithelial-cells-the-impact-on-host-cells
#7
Showgy Y Ma'ayeh, Jingyi Liu, Dimitra Peirasmaki, Katarina Hörnaeus, Sara Bergström Lind, Manfred Grabherr, Jonas Bergquist, Staffan G Svärd
BACKGROUND: Giardia intestinalis is a non-invasive protozoan parasite that causes giardiasis in humans, the most common form of parasite-induced diarrhea. Disease mechanisms are not completely defined and very few virulence factors are known. METHODOLOGY: To identify putative virulence factors and elucidate mechanistic pathways leading to disease, we have used proteomics to identify the major excretory-secretory products (ESPs) when Giardia trophozoites of WB and GS isolates (assemblages A and B, respectively) interact with intestinal epithelial cells (IECs) in vitro...
December 11, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29223801/the-external-face-of-candida-albicans-a-proteomic-view-of-the-cell-surface-and-the-extracellular-environment
#8
Ana Gil-Bona, Ahinara Amador-García, Concha Gil, Lucia Monteoliva
The cell surface and secreted proteins are the initial points of contact between Candida albicans and the host. Improvements in protein extraction approaches and mass spectrometers have allowed researchers to obtain a comprehensive knowledge of these external subproteomes. In this paper, we review the published proteomic studies that have examined C. albicans extracellular proteins, including the cell surface proteins or surfome and the secreted proteins or secretome. The use of different approaches to isolate cell wall and cell surface proteins, such as fractionation approaches or cell shaving, have resulted in different outcomes...
December 6, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29222644/membrane-shed-vesicles-from-the-parasite-trichomonas-vaginalis-characterization-and-their-association-with-cell-interaction
#9
Yesica R Nievas, Veronica M Coceres, Victor Midlej, Wanderley de Souza, Marlene Benchimol, Antonio Pereira-Neves, Ajay A Vashisht, James A Wohlschlegel, Patricia J Johnson, Natalia de Miguel
Trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogenital tract, where it remains extracellular and adheres to epithelial cells. Infections range from asymptomatic to highly inflammatory, depending on the host and the parasite strain. Despite the serious consequences associated with trichomoniasis disease, little is known about parasite or host factors involved in attachment of the parasite-to-host epithelial cells. Here, we report the identification of microvesicle-like structures (MVs) released by T...
December 8, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29221141/plasma-derived-extracellular-vesicle-proteins-as-a-source-of-biomarkers-for-lung-adenocarcinoma
#10
Jody Vykoukal, Nan Sun, Clemente Aguilar-Bonavides, Hiroyuki Katayama, Ichidai Tanaka, Johannes F Fahrmann, Michela Capello, Junya Fujimoto, Mitzi Aguilar, Ignacio I Wistuba, Ayumu Taguchi, Edwin J Ostrin, Samir M Hanash
Exosomes and other extracellular vesicles (EVs) have been implicated as mediators of intercellular communication. Their release into the circulation has the potential to inform about tumor status. In-depth proteomic characterization of plasma-derived EVs has been limited by challenges in isolating EVs from protein-abundant biological fluids. We implemented a novel single-step density gradient flotation workflow for efficient and rapid isolation of highly enriched circulating EVs from plasma. Mass-spectrometry analysis of plasma EVs from subjects with lung adenocarcinoma and matched controls resulted in the identification of 640 proteins...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29209607/a-perspective-on-extracellular-vesicles-proteomics
#11
REVIEW
Livia Rosa-Fernandes, Victória Bombarda Rocha, Victor Corasolla Carregari, Andrea Urbani, Giuseppe Palmisano
Increasing attention has been given to secreted extracellular vesicles (EVs) in the past decades, especially in the portrayal of their molecular cargo and role as messengers in both homeostasis and pathophysiological conditions. This review presents the state-of-the-art proteomic technologies to identify and quantify EVs proteins along with their PTMs, interacting partners and structural details. The rapid growth of mass spectrometry-based analytical strategies for protein sequencing, PTMs and structural characterization has improved the level of molecular details that can be achieved from limited amount of EVs isolated from different biological sources...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/29209155/optimization-of-quantitative-proteomic-analysis-of-clots-generated-from-plasma-of-patients-with-venous-thromboembolism
#12
Aneta Stachowicz, Jakub Siudut, Maciej Suski, Rafał Olszanecki, Ryszard Korbut, Anetta Undas, Jacek R Wiśniewski
Background: It is well known that fibrin network binds a large variety of proteins, including inhibitors and activators of fibrinolysis, which may affect clot properties, such as stability and susceptibility to fibrinolysis. Specific plasma clot composition differs between individuals and may change in disease states. However, the plasma clot proteome has not yet been in-depth analyzed, mainly due to technical difficulty related to the presence of a highly abundant protein-fibrinogen and fibrin that forms a plasma clot...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/29207636/characterization-of-urinary-extracellular-vesicle-proteins-in-muscle-invasive-bladder-cancer
#13
Christopher R Silvers, Hiroshi Miyamoto, Edward M Messing, George J Netto, Yi-Fen Lee
The mechanisms of bladder cancer progression are unknown, and new treatments and biomarkers are needed. Patient urinary extracellular vesicles (EVs) derive in part from bladder cancer cells and contain a specific protein cargo which may provide information about the disease. We conducted a proteomics study comparing EVs from the muscle-invasive bladder cancer (MIBC) cell line TCCSUP to EVs from normal urothelial line SVHUC. GO term analysis showed that TCCSUP EVs are enriched in proteins associated with the cell membrane, extracellular matrix, and inflammation and angiogenesis signaling pathways...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29172549/proteomic-characterization-of-dermal-interstitial-fluid-extracted-using-a-novel-microneedle-assisted-technique
#14
Bao Quoc Tran, Philip R Miller, Robert M Taylor, Gabrielle Boyd, Phillip M Mach, C Nicole Rosenzweig, Justin T Baca, Ronen Polsky, Trevor Glaros
As commercial wearable fitness devices have gained commercial acceptance, interest in-real time monitoring of an individual's physiological status using non-invasive techniques has grown. Microneedles have been proposed as a minimally invasive technique for sampling the dermal interstitial fluid (ISF) for clinical monitoring and diagnosis, but little is known about its composition. In this study, dermal ISF was collected using novel microneedle array from three healthy human donors and compared to matching serum and plasma samples...
November 24, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29145622/plant-extracellular-vesicles-are-incorporated-by-a-fungal-pathogen-and-inhibit-its-growth
#15
Mariana Regente, Marcela Pinedo, Hélène San Clemente, Thierry Balliau, Elisabeth Jamet, Laura de la Canal
Extracellular vesicles (EV) are membrane particles released by cells into their environment and are considered to be key players in intercellular communication. EV are produced by all domains of life but limited knowledge about EV in plants is available, although their implication in plant defense has been suggested. We have characterized sunflower EV and tested whether they could interact with fungal cells. EV were isolated from extracellular fluids of seedlings and characterized by transmission electron microscopy and proteomic analysis...
November 14, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29127410/ultrafiltration-combined-with-size-exclusion-chromatography-efficiently-isolates-extracellular-vesicles-from-cell-culture-media-for-compositional-and-functional-studies
#16
Birke J Benedikter, Freek G Bouwman, Tanja Vajen, Alexandra C A Heinzmann, Gert Grauls, Edwin C Mariman, Emiel F M Wouters, Paul H Savelkoul, Carmen Lopez-Iglesias, Rory R Koenen, Gernot G U Rohde, Frank R M Stassen
Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease. We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies. Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29115712/proteomic-profiling-of-exosomes-secreted-by-breast-cancer-cells-with-varying-metastatic-potential
#17
Lahiru Gangoda, Michael Liem, Ching-Seng Ang, Shivakumar Keerthikumar, Christopher G Adda, Belinda S Parker, Suresh Mathivanan
Cancer cells actively release extracellular vesicles, including exosomes, into the surrounding microenvironment. Exosomes play pleiotropic roles in cancer progression and metastasis, including invasion, angiogenesis, and immune modulation. However, the proteome profile of exosomes isolated from cells with different metastatic potential and the role of these exosomes in driving metastasis remains unclear. Here, we conducted a comparative proteomic analysis of exosomes isolated from several genetically related mouse breast tumour lines with different metastatic propensity...
November 8, 2017: Proteomics
https://www.readbyqxmd.com/read/29114223/the-human-amnion-epithelial-cell-secretome-decreases-hepatic-fibrosis-in-mice-with-chronic-liver-fibrosis
#18
Majid Alhomrani, Jeanne Correia, Marcus Zavou, Bryan Leaw, Nathan Kuk, Rong Xu, Mohamed I Saad, Alexander Hodge, David W Greening, Rebecca Lim, William Sievert
Background: Hepatic stellate cells (HSCs) are the primary collagen-secreting cells in the liver. While HSCs are the major cell type involved in the pathogenesis of liver fibrosis, hepatic macrophages also play an important role in mediating fibrogenesis and fibrosis resolution. Previously, we observed a reduction in HSC activation, proliferation, and collagen synthesis following exposure to human amnion epithelial cells (hAEC) and hAEC-conditioned media (hAEC-CM). This suggested that specific factors secreted by hAEC might be effective in ameliorating liver fibrosis...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29108957/matrix-vesicles-from-chondrocytes-and-osteoblasts-their-biogenesis-properties-functions-and-biomimetic-models
#19
REVIEW
Massimo Bottini, Saida Mebarek, Karen L Anderson, Agnieszka Strzelecka-Kiliszek, Lukasz Bozycki, Ana Maria Sper Simão, Maytê Bolean, Pietro Ciancaglini, Joanna Bandorowicz Pikula, Slawomir Pikula, David Magne, Niels Volkmann, Dorit Hanein, José Luis Millán, Rene Buchet
BACKGROUND: Matrix vesicles (MVs) are released from hypertrophic chondrocytes and from mature osteoblasts, the cells responsible for endochondral and membranous ossification. Under pathological conditions, they can also be released from cells of non-skeletal tissues such as vascular smooth muscle cells. MVs are extracellular vesicles of approximately 100-300nm diameter harboring the biochemical machinery needed to induce mineralization. SCOPE OF THE REVIEW: The review comprehensively delineates our current knowledge of MV biology and highlights open questions aiming to stimulate further research...
November 3, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29093555/exosomes-derived-from-hiv-1-infected-dcs-mediate-viral-trans-infection-via-fibronectin-and-galectin-3
#20
Rutuja Kulkarni, Anil Prasad
Exosomes are membrane enclosed nano-sized vesicles actively released into the extracellular milieu that can harbor genomic, proteomic and lipid cargos. Functionally, they are shown to regulate cell-cell communication and transmission of pathogens. Though studies have implicated a role for exosomes in HIV-1 pathogenesis, their mechanisms are not well defined. Here, we characterized exosomes derived from uninfected or HIV-1 infected T-cells and DCs. We demonstrate substantial differences in morphological, molecular and biogenesis machinery between exosomes derived from these two immune cell types...
November 1, 2017: Scientific Reports
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