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proteomics extracellular vesicle

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https://www.readbyqxmd.com/read/28811546/serum-derived-extracellular-vesicles-evs-impact-on-vascular-remodeling-and-prevent-muscle-damage-in-acute-hind-limb-ischemia
#1
Claudia Cavallari, Andrea Ranghino, Marta Tapparo, Massimo Cedrino, Federico Figliolini, Cristina Grange, Valentina Giannachi, Paolo Garneri, Maria Chiara Deregibus, Federica Collino, Pietro Rispoli, Giovanni Camussi, Maria Felice Brizzi
Serum is an abundant and accessible source of circulating extracellular vesicles (EVs). Serum-EV (sEV) pro-angiogenic capability and mechanisms are herein analyzed using an in vitro assay which predicts sEV angiogenic potential in vivo. Effective sEVs (e-sEVs) also improved vascular remodeling and prevented muscle damage in a mouse model of acute hind limb ischemia. e-sEV angiogenic proteomic and transcriptomic analyses show a positive correlation with matrix-metalloproteinase activation and extracellular matrix organization, cytokine and chemokine signaling pathways, Insulin-like Growth Factor and platelet pathways, and Vascular Endothelial Growth Factor signaling...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28804598/a-rigorous-method-to-enrich-for-exosomes-from-brain-tissue
#2
Laura J Vella, Benjamin J Scicluna, Lesley Cheng, Emma G Bawden, Colin L Masters, Ching-Seng Ang, Nicholas Willamson, Catriona McLean, Kevin J Barnham, Andrew F Hill
Extracellular vesicles, including exosomes, are released by all cells, including those of the nervous system. Capable of delivering lipid, protein and nucleic acids to both nearby and distal cells, exosomes have been hypothesized to play a role in progression of many diseases of the nervous system. To date, most analyses on the role of these vesicles in the healthy and diseased state have relied on studying vesicles from in vitro sources, such as conditioned cell culture media, or body fluids. Here we have taken a critical approach to the enrichment and characterization of exosomes from human frontal cortex...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28780902/emerging-ms-based-platforms-for-the-characterization-of-tumor-derived-exosomes-isolated-from-human-biofluids-challenges-and-promises-of-mudpit
#3
Emanuele Ferrari, Antonella De Palma, Pierluigi Mauri
Exosomes are small extracellular vesicles of endosomal origin that are produced and released by several type of cells. These vesicles contain different macromolecules: proteins, mRNA, miRNA, mitochondrial DNA, and lipids. Exosomes play an important role in cell-to-cell communication, also promoting cancer progression. Areas covered: Various proteomic approaches have been applied to study exosomes isolated from different human biofluids in search of possible cancer biomarkers. The results of these studies are reported, and pros and cons of each employed technique are described...
August 7, 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/28762517/immunoaffinity-based-methods-are-superior-to-kits-for-purification-of-prostate-derived-extracellular-vesicles-from-plasma-samples
#4
Sabine I Brett, Fabrice Lucien, Charles Guo, Karla C Williams, Yohan Kim, Paul N Durfee, C J Brinker, Joseph I Chin, Jun Yang, Hon S Leong
BACKGROUND: The ability to isolate extracellular vesicles (EVs) such as exosomes or microparticles is an important method that is currently not standardized. While commercially available kits offer purification of EVs from biofluids, such purified EV samples will also contain non-EV entities such as soluble protein and nucleic acids that could confound subsequent experimentation. Ideally, only EVs would be isolated and no soluble protein would be present in the final EV preparation. METHODS: We compared commercially available EV isolation kits with immunoaffinity purification techniques and evaluated our final EV preparations using atomic force microscopy (AFM) and nanoscale flow cytometry (NFC)...
August 1, 2017: Prostate
https://www.readbyqxmd.com/read/28761153/pla2r-binds-to-the-annexin-a2-s100a10-complex-in-human-podocytes
#5
Maryline Fresquet, Thomas A Jowitt, Edward A McKenzie, Matthew D Ball, Michael J Randles, Rachel Lennon, Paul E Brenchley
Phospholipase A2 receptor (PLA2R) is a member of the mannose receptor family found in podocytes in human kidney. PLA2R is the target of the autoimmune disease, membranous nephropathy, characterised by production of anti-PLA2R autoantibodies which bind to the podocyte. However the function of PLA2R in health and in disease remains unclear. To gain insight into the molecular mechanisms of PLA2R function, we searched for its endogenous binding partners. Proteomic analysis identified annexinA2 as a potential interactor with the extracellular domains of PLA2R...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28725021/bovine-milk-derived-exosomes-from-colostrum-are-enriched-with-proteins-implicated-in-immune-response-and-growth
#6
Monisha Samuel, David Chisanga, Michael Liem, Shivakumar Keerthikumar, Sushma Anand, Ching-Seng Ang, Christopher G Adda, Ellen Versteegen, Markandeya Jois, Suresh Mathivanan
Exosomes are extracellular vesicles secreted by multiple cell types into the extracellular space. They contain cell-state specific cargos which often reflects the (patho)physiological condition of the cells/organism. Milk contains high amounts of exosomes and it is unclear whether their cargo is altered based on the lactation stage of the organism. Here, we isolated exosomes from bovine milk that were obtained at various stages of lactation and examined the content by quantitative proteomics. Exosomes were isolated by OptiPrep density gradient centrifugation from milk obtained from cow after 24, 48 and 72 h post calving...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28717416/feasibility-of-urinary-extracellular-vesicle-proteome-profiling-using-a-robust-and-simple-clinically-applicable-isolation-method
#7
Irene V Bijnsdorp, Olga Maxouri, Aarzo Kardar, Tim Schelfhorst, Sander R Piersma, Thang V Pham, Andre Vis, R Jeroen van Moorselaar, Connie R Jimenez
Extracellular vesicles (EVs) secreted by prostate cancer (PCa) cells contain specific biomarkers and can be isolated from urine. Collection of urine is not invasive, and therefore urinary EVs represent a liquid biopsy for diagnostic and prognostic testing for PCa. In this study, we optimised urinary EV isolation using a method based on heat shock proteins and compared it to gold-standard ultracentrifugation. The urinary EV isolation protocol using the Vn96-peptide is easier, time convenient (≈1.5 h) and no special equipment is needed, in contrast to ultracentrifugation protocol (>3...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28714175/schwann-cells-secrete-extracellular-vesicles-to-promote-and-maintain-the-proliferation-and-multipotency-of-hdpcs
#8
Ziyue Li, Yan Liang, Kuangwu Pan, Hui Li, Mei Yu, Weihua Guo, Guoqing Chen, Weidong Tian
OBJECTIVES: Schwann cells (SCs) are the principal glial cells in peripheral nerve system, involved in neuropathies with great regenerative potential. Dental pulp cells have been reported to maintain neurogenic potential. In contrast, the regulatory role of SCs on human dental pulp cells (hDPCs) development remains undefined. MATERIALS AND METHODS: SC secretion and SC-derived extracellular vesicles (EVs) were collected and used to treat hDPCs; and proliferation and multiple differentiation of hDPCs were detected after EVs treatments...
August 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28687758/directional-exosome-proteomes-reflect-polarity-specific-functions-in-retinal-pigmented-epithelium-monolayers
#9
Mikael Klingeborn, W Michael Dismuke, Nikolai P Skiba, Una Kelly, W Daniel Stamer, Catherine Bowes Rickman
The retinal pigmented epithelium (RPE) forms the outer blood-retinal barrier in the eye and its polarity is responsible for directional secretion and uptake of proteins, lipoprotein particles and extracellular vesicles (EVs). Such a secretional division dictates directed interactions between the systemic circulation (basolateral) and the retina (apical). Our goal is to define the polarized proteomes and physical characteristics of EVs released from the RPE. Primary cultures of porcine RPE cells were differentiated into polarized RPE monolayers on permeable supports...
July 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28679310/protein-and-molecular-characterisation-of-a-clinically-compliant-amniotic-fluid-stem-cell-derived-extracellular-vesicle-fraction-capable-of-accelerating-muscle-regeneration-through-the-enhancement-of-angiogenesis
#10
Ben Mellows, Robert Mitchell, Manuela Antonioli, Oliver Kretz, David Chamber, Marie-Theres Zeuner, Bernd Denecke, Luca Musante, Durrgah Ramachandra, Florence Debacq-Chainiaux, Harry Holthofer, Barbara Joch, Steve Ray, Darius Widera, Anna L David, Tobios B Huber, Joern Dengjel, Paolo De Coppi, Ketan Patel
The secretome of human amniotic fluid stem cells (AFSC) has great potential as a therapeutic agent in regenerative medicine. However it must be produced in a clinically compliant manner before it can be used in humans. Here we developed a means of producing a biologically active secretome from AFSCs that is free of all exogenous molecules. We demonstrate that the full secretome is capable of promoting stem cell proliferation, migration and protection of cells against senescence. Furthermore, it has significant anti-inflammatory properties...
July 5, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28674889/proteomics-analysis-of-circulating-serum-exosomes
#11
Antonius Koller, Purvi Patel, Jenny Kim Kim, Emily I Chen
Proteomics characterization of biofluids, such as urine and plasma, has been explored for the discovery of predictive, prognostic, and mechanistic biomarkers of diseases and tissue injury. Here we describe comprehensive characterization of protein cargos from cell-derived secreted vesicles (extracellular vesicles or exosome) for biomarker discovery using the mass spectrometry-based technology.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28674887/simultaneous-enrichment-of-plasma-extracellular-vesicles-and-glycoproteome-for-studying-disease-biomarkers
#12
Sunil S Adav, Siu Kwan Sze
To detect disease at an early stage and to develop effective disease treatment therapies, reliable biomarkers of diagnosis, disease progression, and its status remain a research priority. A majority of disease pathologies are primarily associated with different subsets of cells of different tissues, discrete compartments, and areas. These subsets of cells release glycoproteins and specific extracellular vesicles (EVs) including microvesicles and exosomes that carry bioactive cargoes of proteins, nucleic acids, and metabolites...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28674884/targeted-approach-for-proteomic-analysis-of-a-hidden-membrane-protein
#13
Tania Martins-Marques, Sandra I Anjo, Teresa Ribeiro-Rodrigues, Bruno Manadas, Henrique Girao
Given the properties of plasma membrane proteins, namely, their hydrophobicity, low solubility, and high resistance to digestion and extraction, their identification by traditional mass spectrometry (MS) has been a challenging task. Hence, proteomic studies involving the transmembrane protein connexin43 (Cx43) are scarce. Additionally, studies demonstrating the presence of proteins embedded in the lipid bilayer of extracellular vesicles (EVs) are difficult to perform and require specific changes and fine adjustments in the experimental and technical procedure to allow their detection by MS...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28674883/proteomic-analysis-of-blood-extracellular-vesicles-in-cardiovascular-disease-by-lc-ms-ms-analysis
#14
Montserrat Baldan-Martin, Fernando de la Cuesta, Gloria Alvarez-Llamas, Gema Ruiz-Hurtado, Luis M Ruilope, Maria G Barderas
Extracellular vesicles are membrane vesicles related to cell communication. These vesicles consist of proteins, RNA, and microRNA and are an interesting and important tool to understand the processes taking place in the secreting cell, especially in diseases in which its release is often enhanced. The used of blood extracellular vesicles in cardiovascular disease as a low invasive, easily accessible source of circulating markers could give us important information related to pathological process even more with the use of proteomic analysis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28669749/extracellular-vesicles-in-gastrointestinal-cancer-in-conjunction-with-microbiota-on-the-border-of-kingdoms
#15
REVIEW
Natasha S Barteneva, Yeldar Baiken, Elizaveta Fasler-Kan, Kenneth Alibek, Sheng Wang, Natalia Maltsev, Eugene D Ponomarev, Zarina Sautbayeva, Sholpan Kauanova, Anna Moore, Christoph Beglinger, Ivan A Vorobjev
Extracellular vesicle (EV) production is a universal feature of metazoan cells as well as prokaryotes (bMVs - bacterial microvesicles). They are small vesicles with phospholipid membrane carrying proteins, DNA and different classes of RNAs and are heavily involved in intercellular communication acting as vectors of information to target cells. For the last decade, the interest in EV research has exponentially increased though thorough studies of their roles in various pathologies that was not previously possible due to technical limitations...
June 29, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28660234/isolation-of-exosomes-from-semen-for-in-vitro-uptake-and-hiv-1-infection-assays
#16
Marisa N Madison, Jennifer L Welch, Chioma M Okeoma
Exosomes are membranous extracellular nanovesicles of endocytic origin. Exosomes are known to carry host and pathogen-derived genomic, proteomic, lipidomic cargos and other extraneous molecules. Exosomes are secreted by diverse cell types into the extracellular milieu and are subsequently internalized by recipient neighboring or distal cells. Upon internalization, exosomes condition recipient cells by donating their cargos and/or activating various signal transduction pathways, consequently regulating physiological and pathophysiological processes...
April 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28643940/proteomic-profiling-of-extracellular-vesicles-secreted-from-toxoplasma-gondii
#17
Pryscilla Fanini Wowk, Maria Luisa Zardo, Halisson Tesseroli Miot, Samuel Goldenberg, Paulo Costa Carvalho, Patricia Alves Mörking
Toxoplasma gondii infects a wide range of hosts worldwide, including humans and domesticated animals causing toxoplasmosis disease. Recently, exosomes, small extracellular vesicles (EV) that contain nucleic acids, proteins, and lipids derived from their original cells were linked with disease protection. The effect of EVs derived from T. gondii on the immune response and its relevance in a physiological context is unknown. Here we disclose the first proteomic profiling of T. gondii EVs compared to EVs isolated from a human foreskin fibroblast infected cell line cultured in a vesicle- free medium...
June 23, 2017: Proteomics
https://www.readbyqxmd.com/read/28618037/hydrogen-peroxide-stimulates-exosomal-cathepsin-b-regulation-of-the-receptor-for-advanced-glycation-end-products-rage
#18
Charles A Downs, Viet D Dang, Nicholle M Johnson, Nancy Denslow, Abdel A Alli
Exosomes are nano-sized vesicles that are secreted into the extracellular environment. These vesicles contain various biological effector molecules that can regulate intracellular signaling pathways in recipient cells. The aim of this study was to examine a correlation between exosomal cathepsin B activity and the receptor for advanced glycation end-products (RAGE). Type 1 alveolar epithelial (R3/1) cells were treated with or without hydrogen peroxide and exosomes isolated from the cell conditioned media were characterized by NanoSight analysis...
June 15, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28606449/transfusion-related-immunomodulation-and-cancer
#19
REVIEW
Hadi Goubran, David Sheridan, Julia Radosevic, Thierry Burnouf, Jerard Seghatchian
Blood and blood-component therapy triggers immunological reactions in recipients. Transfusion-related immunomodulation [TRIM] is an important complex biological immune reaction to transfusion culminating in immunosuppression. The mechanisms underlying TRIM include the presence of residual leukocytes and apoptotic cells, the transfusion of immunosuppressive cytokines either present in donor components or generated during blood processing, the transfer of metabolically active growth factor-loaded microparticles and extracellular vesicles and the presence of free hemoglobin or extracellular vesicle-bound hemoglobin...
May 27, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28596424/s100-a9-protein-in-exosomes-from-chronic-lymphocytic-leukemia-cells-promotes-nf-%C3%AE%C2%BAb-activity-during-disease-progression
#20
Daniel Prieto, Natalia Sotelo, Noé Seija, Sandra Sernbo, Cecilia Abreu, Rosario Durán, Magdalena Gil, Estefanía Sicco, Victoria Irigoin, Carolina Oliver, Ana Inés Landoni, Raúl Gabus, Guillermo Dighiero, Pablo Oppezzo
Chronic lymphocytic leukemia (CLL) is an incurable disease characterized by accumulation of clonal B lymphocytes, resulting from a complex balance between cell proliferation and apoptotic death. Continuous crosstalk between cancer cells and local/distant host environment is required for effective tumor growth. Among the main actors of this dynamic interplay between tumoral cells and their microenvironment are the nano-sized vesicles called exosomes. Emerging evidence indicates that secretion, composition, and functional capacity of exosomes are altered as tumors progress to an aggressive phenotype...
August 10, 2017: Blood
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