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alveolar macrophage extracellular vesicles

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https://www.readbyqxmd.com/read/28740179/legionella-pneumophila-infection-activates-bystander-cells-differentially-by-bacterial-and-host-cell-vesicles
#1
Anna Lena Jung, Christina Elena Herkt, Christine Schulz, Kathrin Bolte, Kerstin Seidel, Nicoletta Scheller, Alexandra Sittka-Stark, Wilhelm Bertrams, Bernd Schmeck
Extracellular vesicles from eukaryotic cells and outer membrane vesicles (OMVs) released from gram-negative bacteria have been described as mediators of pathogen-host interaction and intercellular communication. Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. The differential effect of bacterial and host cell vesicles in L. pneumophila infection is unknown so far. We infected THP-1-derived or primary human macrophages with L. pneumophila and isolated supernatant vesicles by differential centrifugation...
July 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28598224/mesenchymal-stromal-cells-modulate-macrophages-in-clinically-relevant-lung-injury-models-by-extracellular-vesicle-mitochondrial-transfer
#2
Thomas J Morrison, Megan V Jackson, Erin K Cunningham, Adrien Kissenpfennig, Daniel F McAuley, Cecilia M O'Kane, Anna D Krasnodembskaya
RATIONALE: Acute Respiratory Distress Syndrome (ARDS) remains a major cause of respiratory failure in critically ill patients. Mesenchymal Stromal Cells (MSCs) are a promising candidate for a cell based therapy. However, the mechanisms of MSCs effects in ARDS are not well understood. Here we focused on the paracrine effect of MSCs on macrophage polarization and the role of extracellular vesicle (EV)-mediated mitochondrial transfer. OBJECTIVES: To determine the effects of human MSCs on macrophage function in the ARDS environment and to elucidate the mechanisms of these effects...
June 9, 2017: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/28287548/legionella-pneumophila-outer-membrane-vesicles-isolation-and-analysis-of-their-pro-inflammatory-potential-on-macrophages
#3
Anna Lena Jung, Kerstin Hoffmann, Christina E Herkt, Christine Schulz, Wilhelm Bertrams, Bernd Schmeck
Bacteria are able to secrete a variety of molecules via various secretory systems. Besides the secretion of molecules into the extracellular space or directly into another cell, Gram-negative bacteria can also form outer membrane vesicles (OMVs). These membrane vesicles can deliver their cargo over long distances, and the cargo is protected from degradation by proteases and nucleases. Legionella pneumophila (L. pneumophila) is an intracellular, Gram-negative pathogen that causes a severe form of pneumonia. In humans, it infects alveolar macrophages, where it blocks lysosomal degradation and forms a specialized replication vacuole...
February 22, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28274991/macrophage-derived-apoptotic-bodies-promote-the-proliferation-of-the-recipient-cells-via-shuttling-microrna-221-222
#4
Ziwen Zhu, Duo Zhang, Heedoo Lee, Aravind Ajakumar Menon, Jingxuan Wu, Kebin Hu, Yang Jin
Bacterial pneumonia is a common and serious clinical entity. Alveolar epithelial cells and alveolar macrophages are the first line of defense in the innate immunity against bacterial pathogens. Epithelial cells are known to release chemokines/cytokines that recruit and activate phagocytic cells. However, the signals sent from alveolar macrophages back to the lung epithelial cells remain largely unexplored. We found that LPS, a well-recognized stimulator derived from gram-negative (G(-)) bacteria, rapidly and robustly induces the secretion of macrophage-derived extracellular vesicles (EVs)...
June 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/27731391/epithelial-cell-derived-microvesicles-activate-macrophages-and-promote-inflammation-via-microvesicle-containing-micrornas
#5
Heedoo Lee, Duo Zhang, Ziwen Zhu, Charles S Dela Cruz, Yang Jin
Intercellular communications between lung epithelial cells and alveolar macrophages play an essential role in host defense against acute lung injury. Hyperoxia-induced oxidative stress is an established model to mimic human lung injury. We show that after hyperoxia-associated oxidative stress, a large amount of extracellular vesicles (EVs) are detectable in bronchoalveolar lavage fluid (BALF) and culture medium of lung epithelial cells. Microvesicles (MVs), but not exosomes (Exos) or apoptotic bodies (Abs), are the main type of EVs found in the early stages after hyperoxia...
October 12, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27626032/signed-sealed-delivered-microenvironmental-modulation-of-extracellular-vesicle-dependent-immunoregulation-in-the-lung
#6
REVIEW
Daniel J Schneider, Jennifer M Speth, Marc Peters-Golden
Unconventional secretion and subsequent uptake of molecular cargo via extracellular vesicles (EVs) is an important mechanism by which cells can exert paracrine effects. While this phenomenon has been widely characterized in the context of their ability to promote inflammation, less is known about the ability of EVs to transfer immunosuppressive cargo. Maintenance of normal physiology in the lung requires suppression of potentially damaging inflammatory responses to the myriad of insults to which it is continually exposed...
2016: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/26658190/lung-epithelial-cell-derived-extracellular-vesicles-activate-macrophage-mediated-inflammatory-responses-via-rock1-pathway
#7
H-G Moon, Y Cao, J Yang, J H Lee, H S Choi, Y Jin
Despite decades of research, the pathogenesis of acute respiratory distress syndrome (ARDS) remains poorly understood, thus impeding the development of effective treatment. Diffuse alveolar damage (DAD) and lung epithelial cell death are prominent features of ARDS. Lung epithelial cells are the first line of defense after inhaled stimuli, such as in the case of hyperoxia. We hypothesized that lung epithelial cells release 'messenger' or signaling molecules to adjacent or distant macrophages, thereby initiating or propagating inflammatory responses after noxious insult...
December 10, 2015: Cell Death & Disease
https://www.readbyqxmd.com/read/26109643/bacterial-membrane-vesicles-mediate-the-release-of-mycobacterium-tuberculosis-lipoglycans-and-lipoproteins-from-infected-macrophages
#8
Jaffre J Athman, Ying Wang, David J McDonald, W Henry Boom, Clifford V Harding, Pamela A Wearsch
Mycobacterium tuberculosis is an intracellular pathogen that infects lung macrophages and releases microbial factors that regulate host defense. M. tuberculosis lipoproteins and lipoglycans block phagosome maturation, inhibit class II MHC Ag presentation, and modulate TLR2-dependent cytokine production, but the mechanisms for their release during infection are poorly defined. Furthermore, these molecules are thought to be incorporated into host membranes and released from infected macrophages within exosomes, 40-150-nm extracellular vesicles that derive from multivesicular endosomes...
August 1, 2015: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/25716999/extracellular-vesicles-derived-from-gram-negative-bacteria-such-as-escherichia-coli-induce-emphysema-mainly-via-il-17a-mediated-neutrophilic-inflammation
#9
You-Sun Kim, Won-Hee Lee, Eun-Jeong Choi, Jun-Pyo Choi, Young Joo Heo, Yong Song Gho, Young-Koo Jee, Yeon-Mok Oh, Yoon-Keun Kim
Recent evidence indicates that Gram-negative bacteria-derived extracellular vesicles (EVs) in indoor dust can evoke neutrophilic pulmonary inflammation, which is a key pathology of chronic obstructive pulmonary disease (COPD). Escherichia coli is a ubiquitous bacterium present in indoor dust and secretes nanometer-sized vesicles into the extracellular milieu. In the current study, we evaluated the role of E. coli-derived EVs on the development of COPD, such as emphysema. E. coli EVs were prepared by sequential ultrafiltration and ultracentrifugation...
April 1, 2015: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/25063875/adam9-is-a-novel-product-of-polymorphonuclear-neutrophils-regulation-of-expression-and-contributions-to-extracellular-matrix-protein-degradation-during-acute-lung-injury
#10
Robin Roychaudhuri, Anja H Hergrueter, Francesca Polverino, Maria E Laucho-Contreras, Kushagra Gupta, Niels Borregaard, Caroline A Owen
A disintegrin and a metalloproteinase domain (ADAM) 9 is known to be expressed by monocytes and macrophages. In this study, we report that ADAM9 is also a product of human and murine polymorphonuclear neutrophils (PMNs). ADAM9 is not synthesized de novo by circulating PMNs. Rather, ADAM9 protein is stored in the gelatinase and specific granules and the secretory vesicles of human PMNs. Unstimulated PMNs express minimal quantities of surface ADAM9, but activation of PMNs with degranulating agonists rapidly (within 15 min) increases PMN surface ADAM9 levels...
September 1, 2014: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/24166955/human-lung-tissue-explants-reveal-novel-interactions-during-legionella-pneumophila-infections
#11
Jens Jäger, Sebastian Marwitz, Jana Tiefenau, Janine Rasch, Olga Shevchuk, Christian Kugler, Torsten Goldmann, Michael Steinert
Histological and clinical investigations describe late stages of Legionnaires' disease but cannot characterize early events of human infection. Cellular or rodent infection models lack the complexity of tissue or have nonhuman backgrounds. Therefore, we developed and applied a novel model for Legionella pneumophila infection comprising living human lung tissue. We stimulated lung explants with L. pneumophila strains and outer membrane vesicles (OMVs) to analyze tissue damage, bacterial replication, and localization as well as the transcriptional response of infected tissue...
January 2014: Infection and Immunity
https://www.readbyqxmd.com/read/23517040/extracellular-vesicles-especially-derived-from-gram-negative-bacteria-in-indoor-dust-induce-neutrophilic-pulmonary-inflammation-associated-with-both-th1-and-th17-cell-responses
#12
Y-S Kim, E-J Choi, W-H Lee, S-J Choi, T-Y Roh, J Park, Y-K Jee, Z Zhu, Y-Y Koh, Y S Gho, Y-K Kim
BACKGROUND: Many bacterial components in indoor dust can evoke inflammatory pulmonary diseases. Bacteria secrete nanometre-sized vesicles into the extracellular milieu, but it remains to be determined whether bacteria-derived extracellular vesicles in indoor dust are pathophysiologically related to inflammatory pulmonary diseases. OBJECTIVE: To evaluate whether extracellular vesicles (EV) in indoor air are related to the pathogenesis of pulmonary inflammation and/or asthma...
April 2013: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/23436998/intracellular-bacillary-burden-reflects-a-burst-size-for-mycobacterium-tuberculosis-in-vivo
#13
Teresa Repasy, Jinhee Lee, Simeone Marino, Nuria Martinez, Denise E Kirschner, Gregory Hendricks, Stephen Baker, Andrew A Wilson, Darrell N Kotton, Hardy Kornfeld
We previously reported that Mycobacterium tuberculosis triggers macrophage necrosis in vitro at a threshold intracellular load of ~25 bacilli. This suggests a model for tuberculosis where bacilli invading lung macrophages at low multiplicity of infection proliferate to burst size and spread to naïve phagocytes for repeated cycles of replication and cytolysis. The current study evaluated that model in vivo, an environment significantly more complex than in vitro culture. In the lungs of mice infected with M...
February 2013: PLoS Pathogens
https://www.readbyqxmd.com/read/22913540/staphylococcus-aureus-derived-extracellular-vesicles-induce-neutrophilic-pulmonary-inflammation-via-both-th1-and-th17-cell-responses
#14
M-R Kim, S-W Hong, E-B Choi, W-H Lee, Y-S Kim, S G Jeon, M H Jang, Y S Gho, Y-K Kim
BACKGROUND: Recent evidence indicates that Staphylococcus aureus, one of the most important human pathogens, secretes vesicles into the extracellular milieu. OBJECTIVE: To evaluate whether inhalation of S. aureus-derived extracellular vesicles (EV) is causally related to the pathogenesis of inflammatory pulmonary diseases. METHODS: Staphylococcus aureus EV were prepared by sequential ultrafiltration and ultracentrifugation. The innate immune response was evaluated in vitro after the application of EV to airway epithelial cells and alveolar macrophages...
October 2012: Allergy
https://www.readbyqxmd.com/read/22674628/nanoparticle-induced-exosomes-target-antigen-presenting-cells-to-initiate-th1-type-immune-activation
#15
Motao Zhu, Xin Tian, Xiao Song, Yiye Li, Yanhua Tian, Yuliang Zhao, Guangjun Nie
The mechanisms associated with the induction of systemic immune responses by nanoparticles are not fully understood, but their elucidation is critical to address safety issues associated with the broader medical application of nanotechnology. In this study, a key role of nanoparticle-induced exosomes (extracellularly secreted membrane vesicles) as signaling mediators in the induction of T helper cell type 1 (Th1) immune activation is demonstrated. In vivo exposure to magnetic iron oxide nanoparticles (MIONs) results in significant exosome generation in the alveolar region of Balb/c mice...
September 24, 2012: Small
https://www.readbyqxmd.com/read/15376627/phospholipid-metabolism-in-lung-surfactant
#16
REVIEW
Ruud Veldhuizen, Fred Possmayer
Pulmonary surfactant is a mixture of lipids, mostly phospholipids, and proteins that allows for breathing with minimal effort. The current chapter discusses the metabolism of the phospholipids of this material. Surfactant phospholipids are synthesized in the type II epithelial cells of the lung. The lipids and surfactant proteins are assembled in intracellular storage organelles, called lamellar bodies, and are subsequently secreted into the alveolar space. Within this extracellular space surfactant undergoes several transformations...
2004: Sub-cellular Biochemistry
https://www.readbyqxmd.com/read/9272930/alterations-of-lung-structure-in-experimental-diabetes-and-diabetes-associated-with-hyperlipidaemia-in-hamsters
#17
D Popov, M Simionescu
Since hyperglycaemia is known to affect normal pulmonary physiology and biochemistry and few structure-function correlations have been reported, we designed experiments on hamsters subjected to streptozotocin-induced diabetes or diabetes associated with hyperlipidaemia, and investigated the impact of these conditions on the lung structure. At time intervals ranging 2-24 weeks from the inception of disease (without correcting blood glucose with insulin), the animals were sacrificed, and plasma glucose and cholesterol assayed...
August 1997: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/8915456/responses-of-pulmonary-intravascular-macrophages-to-915-mhz-microwave-radiation-ultrastructural-and-cytochemical-study
#18
COMPARATIVE STUDY
B Singh, L A Bate
BACKGROUND: Microwave (MW) radiation is being increasingly used as a source of heat supplementation during early postnatal development of pigs. Although MW radiation does not cause deleterious physiological effects, no specific information exists regarding its impact on immune cells such as macrophages. Pulmonary intravascular macrophages (PIMs) are emerging as important inflammatory cells due to their endocytic and secretory potential. An in vivo study was conducted to evaluate the effects of infrared, and low and high power MW radiation on the PIMs of pigs...
November 1996: Anatomical Record
https://www.readbyqxmd.com/read/8143254/porcine-reproductive-and-respiratory-syndrome-virus-morphological-biochemical-and-serological-characteristics-of-quebec-isolates-associated-with-acute-and-chronic-outbreaks-of-porcine-reproductive-and-respiratory-syndrome
#19
H Mardassi, R Athanassious, S Mounir, S Dea
Cytolytic and noncytolytic strains of the porcine reproductive and respiratory syndrome virus (PRRSV) were isolated in primary cultures of porcine alveolar macrophages (PAM) from lung homogenates of stillborn fetuses or blood samples of dyspneic piglets collected from Quebec pig farms having experienced acute or chronic outbreaks of PRRS. Serological identification of the virus was confirmed by indirect immunofluorescence and indirect protein A-gold immunoelectron microscopy using reference antiserum prepared from experimentally-infected specific pathogen free (SPF) piglets and monoclonal antibodies (MoAbs) directed against the p15 nucleocapsid (N) protein of the reference ATCC-VR2332 isolate...
January 1994: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/7328118/exocytosis-of-pinocytosed-fluid-in-cultured-cells-kinetic-evidence-for-rapid-turnover-and-compartmentation
#20
J M Besterman, J A Airhart, R C Woodworth, R B Low
The uptake and fate of pinocytosed fluid were investigated in monolayers of pulmonary alveolar macrophages and fetal lung fibroblasts using the fluid-phase marker, [14C]sucrose. Initial experiments revealed that cellular accumulation of chromatographically repurified [14C]sucrose was not linear with incubation time. Deviation from linearity was shown to be due to constant exocytosis of accumulating marker. Chromatographic analysis revealed that the cells were unable to metabolize sucrose and were releasing it intact by a process that was temperature-sensitive but not dependent on extracellular calcium and magnesium...
December 1981: Journal of Cell Biology
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