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alveolar macrophage proteomics

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https://www.readbyqxmd.com/read/27788778/alcohol-and-lung-injury-and-immunity
#1
REVIEW
Samantha M Yeligar, Michael M Chen, Elizabeth J Kovacs, Joseph H Sisson, Ellen L Burnham, Lou Ann S Brown
Annually, excessive alcohol use accounts for more than $220 billion in economic costs and 80,000 deaths, making excessive alcohol use the third leading lifestyle-related cause of death in the US. Patients with an alcohol-use disorder (AUD) also have an increased susceptibility to respiratory pathogens and lung injury, including a 2-4-fold increased risk of acute respiratory distress syndrome (ARDS). This review investigates some of the potential mechanisms by which alcohol causes lung injury and impairs lung immunity...
September 2016: Alcohol
https://www.readbyqxmd.com/read/27760463/mycobacterium-tuberculosis-infection-manipulates-the-glycosylation-machinery-and-the-n-glycoproteome-of-human-macrophages-and-their-microparticles
#2
Nathan J Hare, Ling Y Lee, Ian Loke, Warwick J Britton, Bernadette M Saunders, Morten Thaysen-Andersen
Tuberculosis (TB) remains a prevalent and lethal infectious disease. The glycobiology associated with Mycobacterium tuberculosis infection of front-line alveolar macrophages is still unresolved. Herein, we investigated the regulation of protein N-glycosylation in human macrophages and their secreted microparticles (MPs) used for intercellular communication upon M. tb infection. LC-MS/MS-based proteomics and glycomics were performed to monitor the regulation of glycosylation enzymes and receptors and the N-glycome in in vitro-differentiated macrophages and in isolated MPs upon M...
October 19, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27447723/involvement-of-exosomes-in-lung-inflammation-associated-with-experimental-acute-pancreatitis
#3
Laia Bonjoch, Vanessa Casas, Montserrat Carrascal, Daniel Closa
A frequent complication of acute pancreatitis is the lung damage associated with the systemic inflammatory response. Although various pro-inflammatory mediators generated at both local and systemic levels have been identified, the pathogenic mechanisms of the disease are still poorly understood. In recent years, exosomes have emerged as a new intercellular communication system able to transfer encapsulated proteins and small RNAs and protect them from degradation. Using an experimental model of taurocholate-induced acute pancreatitis in rats, we aimed to evaluate the role of exosomes in the extent of the systemic inflammatory response...
October 2016: Journal of Pathology
https://www.readbyqxmd.com/read/27055832/alterations-in-the-membrane-associated-proteome-fraction-of-alveolar-macrophages-in-sarcoidosis
#4
Hanna Kjellin, Ernesto Silva, Rui Mamede Branca, Anders Eklund, Per-Johan Jakobsson, Johan Grunewald, Janne Lehtiö, Åsa M Wheelock
BACKGROUND: Alveolar macrophages are implicated in the pathogenesis of lung sarcoidosis. Their interaction with T-cells leads to an inflammatory response that may either resolve within 2 years, or become chronic with an increased risk to develop lung fibrosis. OBJECTIVE: To perform quantitative profiling of the membrane-associated proteome of alveolar macrophages in sarcoidosis patients and healthy individuals to identify specific proteins and pathways involved in sarcoidosis pathology...
2016: Sarcoidosis, Vasculitis, and Diffuse Lung Diseases: Official Journal of WASOG
https://www.readbyqxmd.com/read/26998217/single-cell-analysis-reveals-differential-regulation-of-the-alveolar-macrophage-actin-cytoskeleton-by-surfactant-proteins-a1-and-a2-implications-of-sex-and-aging
#5
Nikolaos Tsotakos, David S Phelps, Christopher M Yengo, Vernon M Chinchilli, Joanna Floros
BACKGROUND: Surfactant protein A (SP-A) contributes to lung immunity by regulating inflammation and responses to microorganisms invading the lung. The huge genetic variability of SP-A in humans implies that this protein is highly important in tightly regulating the lung immune response. Proteomic studies have demonstrated that there are differential responses of the macrophages to SP-A1 and SP-A2 and that there are sex differences implicated in these responses. METHODS: Purified SP-A variants were used for administration to alveolar macrophages from SP-A knockout (KO) mice for in vitro studies, and alveolar macrophages from humanized SP-A transgenic mice were isolated for ex vivo studies...
2016: Biology of Sex Differences
https://www.readbyqxmd.com/read/26829557/ifns-modify-the-proteome-of-legionella-containing-vacuoles-and-restrict-infection-via-irg1-derived-itaconic-acid
#6
Jan Naujoks, Christoph Tabeling, Brian D Dill, Christine Hoffmann, Andrew S Brown, Mareike Kunze, Stefan Kempa, Andrea Peter, Hans-Joachim Mollenkopf, Anca Dorhoi, Olivia Kershaw, Achim D Gruber, Leif E Sander, Martin Witzenrath, Susanne Herold, Andreas Nerlich, Andreas C Hocke, Ian van Driel, Norbert Suttorp, Sammy Bedoui, Hubert Hilbi, Matthias Trost, Bastian Opitz
Macrophages can be niches for bacterial pathogens or antibacterial effector cells depending on the pathogen and signals from the immune system. Here we show that type I and II IFNs are master regulators of gene expression during Legionella pneumophila infection, and activators of an alveolar macrophage-intrinsic immune response that restricts bacterial growth during pneumonia. Quantitative mass spectrometry revealed that both IFNs substantially modify Legionella-containing vacuoles, and comparative analyses reveal distinct subsets of transcriptionally and spatially IFN-regulated proteins...
February 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/26389541/proteomic-and-bioinformatics-profile-of-paired-human-alveolar-macrophages-and-peripheral-blood-monocytes
#7
Sara E Tomechko, Kathleen C Lundberg, Jessica Jarvela, Gurkan Bebek, Nicole G Chesnokov, Daniela Schlatzer, Rob M Ewing, W Henry Boom, Mark R Chance, Richard F Silver
Little is known about proteomic differences between pluripotent human peripheral blood monocytes (MN) and their terminally-differentiated pulmonary counterparts, alveolar macrophages (AM). To better characterize these cell populations, we performed a label-free shotgun proteomics assessment of matched AM and MN preparations from eight healthy volunteers. With an FDR of less than 0.45%, we identified 1754 proteins within AM and 1445 from MN. Comparison of the two proteomes revealed that 1239 of the proteins found in AM were shared with MN, whereas 206 proteins were uniquely identified in MN and 515 were unique to AM...
November 2015: Proteomics
https://www.readbyqxmd.com/read/26376364/a-ccl24-dependent-pathway-augments-eosinophilic-airway-inflammation-in-house-dust-mite-challenged-cd163-mice
#8
C Dai, X Yao, E M Gordon, A Barochia, R A Cuento, M Kaler, K S Meyer, K J Keeran, G Z Nugent, K R Jeffries, X Qu, Z-X Yu, A Aponte, M Gucek, P K Dagur, J P McCoy, S J Levine
CD163 is a macrophage scavenger receptor with anti-inflammatory and pro-inflammatory functions. Here, we report that alveolar macrophages (AMΦs) from asthmatic subjects had reduced cell-surface expression of CD163, which suggested that CD163 might modulate the pathogenesis of asthma. Consistent with this, house dust mite (HDM)-challenged Cd163(-/-) mice displayed increases in airway eosinophils and mucous cell metaplasia (MCM). The increased airway eosinophils and MCM in HDM-challenged Cd163(-/-) mice were mediated by augmented CCL24 production and could be reversed by administration of a neutralizing anti-CCL24 antibody...
May 2016: Mucosal Immunology
https://www.readbyqxmd.com/read/25639505/targeted-mass-spectrometry-for-the-analysis-of-nutritive-modulation-of-catalase-and-heme-oxygenase-1-expression
#9
Nina Zaenglein, Joanna Tucher, Monika Pischetsrieder
UNLABELLED: Comprehensive physiological food assessment requires recording of activity profiles. To elucidate the nutritive regulation of antioxidant enzymes, a generally applicable targeted MS method was established for the expression analysis of catalase and then adapted to heme oxygenase-1. Before tryptic digestion, target proteins were prefractionated by off-gel IEF of stimulated and control cell lysate. Targeted proteome analysis was achieved by LC coupled with scheduled selected reaction monitoring MS using 2 proteotypic peptides per protein and 3-4 transitions per peptide...
March 18, 2015: Journal of Proteomics
https://www.readbyqxmd.com/read/25496424/beyond-rab-gtpases-legionella-activates-the-small-gtpase-ran-to-promote-microtubule-polymerization-pathogen-vacuole-motility-and-infection
#10
Hubert Hilbi, Eva Rothmeier, Christine Hoffmann, Christopher F Harrison
Legionella spp. are amoebae-resistant environmental bacteria that replicate in free-living protozoa in a distinct compartment, the Legionella-containing vacuole (LCV). Upon transmission of Legionella pneumophila to the lung, the pathogens employ an evolutionarily conserved mechanism to grow in LCVs within alveolar macrophages, thus triggering a severe pneumonia termed Legionnaires' disease. LCV formation is a complex and robust process, which requires the bacterial Icm/Dot type IV secretion system and involves the amazing number of 300 different translocated effector proteins...
2014: Small GTPases
https://www.readbyqxmd.com/read/25404120/interference-of-aspergillus-fumigatus-with-the-immune-response
#11
REVIEW
Thorsten Heinekamp, Hella Schmidt, Katrin Lapp, Vera Pähtz, Iordana Shopova, Nora Köster-Eiserfunke, Thomas Krüger, Olaf Kniemeyer, Axel A Brakhage
Aspergillus fumigatus is a saprotrophic filamentous fungus and also the most prevalent airborne fungal pathogen of humans. Depending on the host's immune status, the variety of diseases caused by A. fumigatus ranges from allergies in immunocompetent hosts to life-threatening invasive infections in patients with impaired immunity. In contrast to the majority of other Aspergillus species, which are in most cases nonpathogenic, A. fumigatus features an armory of virulence determinants to establish an infection...
March 2015: Seminars in Immunopathology
https://www.readbyqxmd.com/read/25250244/metabolism-of-the-vacuolar-pathogen-legionella-and-implications-for-virulence
#12
Christian Manske, Hubert Hilbi
Legionella pneumophila is a ubiquitous environmental bacterium that thrives in fresh water habitats, either as planktonic form or as part of biofilms. The bacteria also grow intracellularly in free-living protozoa as well as in mammalian alveolar macrophages, thus triggering a potentially fatal pneumonia called "Legionnaires' disease." To establish its intracellular niche termed the "Legionella-containing vacuole" (LCV), L. pneumophila employs a type IV secretion system and translocates ~300 different "effector" proteins into host cells...
2014: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/24954098/sex-differences-in-the-acute-in-vivo-effects-of-different-human-sp-a-variants-on-the-mouse-alveolar-macrophage-proteome
#13
David S Phelps, Todd M Umstead, Joanna Floros
UNLABELLED: Surfactant protein A (SP-A) is involved in lung innate immunity. Humans have two SP-A genes, SFTPA1 and SFTPA2, each with several variants. We examined the in vivo effects of treatment with specific SP-A variants on the alveolar macrophage (AM) proteome from SP-A knockout (KO) mice. KO mice received either SP-A1, SP-A2, or both. AM were collected and their proteomes examined with 2D-DIGE. We identified 90 proteins and categorized them as related to actin/cytoskeleton, oxidative stress, protease balance/chaperones, regulation of inflammation, and regulatory/developmental processes...
August 28, 2014: Journal of Proteomics
https://www.readbyqxmd.com/read/24729982/differences-in-the-alveolar-macrophage-proteome-in-transgenic-mice-expressing-human-sp-a1-and-sp-a2
#14
David S Phelps, Todd M Umstead, Patricia Silveyra, Sanmei Hu, Guirong Wang, Joanna Floros
Surfactant protein A (SP-A) plays a number of roles in lung host defense and innate immunity. There are two human genes, SFTPA1 and SFTPA2, and evidence indicates that the function of SP-A1 and SP-A2 proteins differ in several respects. To investigate the impact of SP-A1 and SP-A2 on the alveolar macrophage (AM) phenotype, we generated humanized transgenic (hTG) mice on the SP-A knockout (KO) background, each expressing human SP-A1 or SP-A2. Using two-dimensional difference gel electrophoresis (2D-DIGE) we studied the AM cellular proteome...
April 1, 2013: Journal of Proteomics and Genomics Research
https://www.readbyqxmd.com/read/24729918/gender-differences-in-pulmonary-function-respiratory-symptoms-and-macrophage-proteomics-among-hiv-infected-smokers
#15
Shiva D Rahmanian, Karen L Wood, Shili Lin, Mark A King, April Horne, Shangbin Yang, Haifeng M Wu, Philip T Diaz
Background. HIV-infected subjects have an increased incidence of pulmonary emphysema. There are known gender differences in COPD phenotypic expression and diagnosis, but this is not well characterized in lung disease related to HIV. We analyzed a group at risk for the development of COPD (HIV-infected smokers) to determine gender differences in pulmonary symptoms, pulmonary function tests, and HRCT appearances. Methods. This was a cross-sectional, baseline analysis of a prospective study performed between 2006 and 2010...
2014: Scientifica
https://www.readbyqxmd.com/read/24690554/beyond-rab-gtpases-legionella-activates-the-small-gtpase-ran-to-promote-microtubule-polymerization-pathogen-vacuole-motility-and-infection
#16
Hubert Hilbi, Eva Rothmeier, Christine Hoffmann, Christopher F Harrison
Legionella spp. are amoebae-resistant environmental bacteria that replicate in free-living protozoa in a distinct compartment, the Legionella-containing vacuole (LCV). Upon transmission of Legionella pneumophila to the lung, the pathogens employ an evolutionarily conserved mechanism to grow in LCVs within alveolar macrophages, thus triggering a severe pneumonia termed Legionnaires' disease. LCV formation is a complex and robust process, which requires the bacterial Icm/Dot type IV secretion system and involves the amazing number of 300 different translocated effector proteins...
March 31, 2014: Small GTPases
https://www.readbyqxmd.com/read/24533505/label-free-quantitative-phosphoproteomic-analysis-reveals-differentially-regulated-proteins-and-pathway-in-prrsv-infected-pulmonary-alveolar-macrophages
#17
Rui Luo, Liurong Fang, Hui Jin, Dang Wang, Kang An, Ningzhi Xu, Huanchun Chen, Shaobo Xiao
Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen of swine worldwide and causes significant economic losses. Through regulating the host proteins phosphorylation, PRRSV was found to manipulate the activities of several signaling molecules to regulate innate immune responses. However, the role of protein phosphorylation during PRRSV infection and the signal pathways responsible for it are relatively unknown. Here liquid chromatography-tandem mass spectrometry for label-free quantitative phosphoproteomics was applied to systematically investigate the global phosphorylation events in PRRSV-infected pulmonary alveolar macrophages...
March 7, 2014: Journal of Proteome Research
https://www.readbyqxmd.com/read/24465692/identification-of-differentially-expressed-proteins-in-porcine-alveolar-macrophages-infected-with-virulent-attenuated-strains-of-porcine-reproductive-and-respiratory-syndrome-virus
#18
Yan-Jun Zhou, Jian-Ping Zhu, Tao Zhou, Qun Cheng, Ling-Xue Yu, Ya-Xin Wang, Shen Yang, Yi-Feng Jiang, Wu Tong, Fei Gao, Hai Yu, Guo-Xin Li, Guang-Zhi Tong
The highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) is still a serious threat to the swine industry. However, the pathogenic mechanism of HP-PRRSV remains unclear. We infected host porcine alveolar macrophages (PAMs) with the virulent HuN4 strain and the attenuated HuN4-F112 strain and then utilized fluorescent two-dimensional difference gel electrophoresis (2D-DIGE) to screen for intracellular proteins that were differentially expressed in host cells infected with the two strains...
2014: PloS One
https://www.readbyqxmd.com/read/24111704/analysis-of-the-plasma-proteome-in-copd-novel-low-abundance-proteins-reflect-the-severity-of-lung-remodeling
#19
MULTICENTER STUDY
Salim Merali, Carlos A Barrero, Russell P Bowler, Diane Er Chen, Gerard Criner, Alan Braverman, Samuel Litwin, Anthony Yeung, Steven G Kelsen
The search for COPD biomarkers has largely employed a targeted approach that focuses on plasma proteins involved in the systemic inflammatory response and in lung injury and repair. This proof of concept study was designed to test the idea that an open, unbiased, in-depth proteomics approach could identify novel, low abundance plasma proteins i.e., ng/mL concentration, which could serve as potential biomarkers. Differentially expressed proteins were identified in a discovery group with severe COPD (FEV1 <45% predicted; n = 10)...
April 2014: COPD
https://www.readbyqxmd.com/read/23773529/comparative-analysis-of-the-alveolar-macrophage-proteome-in-ali-ards-patients-between-the-exudative-phase-and-recovery-phase
#20
COMPARATIVE STUDY
Haiyun Dong, Jinxiu Li, Youdi Lv, Yanyan Zhou, Guyi Wang, Shuang Hu, Xiaoyu He, Ping Yang, Zhiguang Zhou, Xudong Xiang, Cong-Yi Wang
BACKGROUND: Despite decades of extensive studies, the morbidity and mortality for acute lung injury/acute respiratory distress syndrome (ALI/ARDS) remained high. Particularly, biomarkers essential for its early diagnosis and prognosis are lacking. METHODS: Recent studies suggest that alveolar macrophages (AMs) at the exudative phase of ALI/ARDS initiate, amplify and perpetuate inflammatory responses, while they resolve inflammation in the recovery phase to prevent further tissue injury and perpetuated inflammation in the lung...
2013: BMC Immunology
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