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hashimotos and fertility

Kayo Hashimoto, Shunsuke Miyashima, Kumi Sato-Nara, Toshihiro Yamada, Keiji Nakajima
The ovules of flowering plants consist of a central embryo sac and surrounding layers of the inner and outer integument. As these structural units eventually give rise to the embryo/endosperm and seed coat, respectively, a precisely organized ovule structure is essential for successful fertilization and seed production. In Arabidopsis thaliana, correct ovule patterning depends on the restricted expression of the CLASS III HOMEODOMAIN LEUCINE ZIPPER (HD-ZIP III) gene PHABULOSA (PHB) in the apical region of the incipient inner integument, which in turn is regulated via post-transcriptional suppression by miR165 and miR166 (miR165/6) derived from multiple MIR165/6 genes...
February 16, 2018: Plant & Cell Physiology
Yuko Kitano, Shu Hashimoto, Hiroshi Matsumoto, Takayuki Yamochi, Masaya Yamanaka, Yoshiharu Nakaoka, Aisaku Fukuda, Masayasu Inoue, Tomoaki Ikeda, Yoshiharu Morimoto
Age-dependent decline of mitochondrial function has been proposed to be a main cause of decline of embryo quality. Then, l-carnitine plays important roles in reducing the membranous toxicity of free-fatty acids by forming acyl-carnitine and promoting β-oxidation, preventing cell damage. Recent research revealed that l-carnitine played important roles in vitro in oocyte growth, oocyte maturation and embryo development. However, such beneficial effects of l-carnitine in vivo have yet to be verified. The effect of oral l-carnitine supplementation on embryo quality and implantation potential was examined...
January 29, 2018: Gynecological Endocrinology
Noriyuki Okuyama, Ryuichiro Obata, Nao Oka, Yusuke Nakamura, Hiromitsu Hattori, Yukiko Nakajo, Nobuya Aono, Masae Koizumi, Mayumi Toya, Koichi Nagao, Toshihiro Tai, Tomoko Hashimoto, Hideki Igarashi, Koichi Kyono
Purpose: To find the best methods to achieve the highest pregnancy and birth rates for couples needing testicular sperm extraction (TESE)-intracytoplasmic sperm injection (ICSI). Methods: Retrospectively studied were 801 patients with male factor infertility who had undergone TESE-ICSI between April, 1996 and July, 2016 and who had been categorized into four groups: obstructive azoospermia (OA); non-obstructive azoospermia (NOA); Klinefelter syndrome (KS); and cryptozoospermia (Crypt)...
January 2018: Reproductive Medicine and Biology
Masako Kuroda, Keiji Kuroda, Tomoya Segawa, Jaeduk Y Noh, Ai Yoshihara, Koichi Ito, Hisao Osada, Satoru Takeda, Shokichi Teramoto
AIM: Thyroid dysfunction and autoimmunity are associated with an adverse effect on fertility. An aberrant high thyroid stimulating hormone level is associated with diminished ovarian reserve in women of reproductive age; however, the utility of levothyroxine (LT4) replacement for infertile patients with subclinical hypothyroidism is still under discussion. The aim of this study was to investigate whether LT4 supplementation for infertile patients with subclinical hypothyroidism improves impaired ovarian function...
January 3, 2018: Journal of Obstetrics and Gynaecology Research
Tomoko Hashimoto, Yusuke Nakamura, Ryuichiro Obata, Masakazu Doshida, Mayumi Toya, Takumi Takeuchi, Koichi Kyono
Purpose: To assess the efficacy of fertility preservation (FP) and the impact of chemotherapy on the reproductive potential of Japanese patients with breast cancer. Methods: Sixty-two patients with breast cancer visited the authors' centers from October, 2003 to June, 2015. They were divided into two groups according to the treatment: oocyte or embryo vitrification for FP before cancer treatment (group A) or infertility treatment after cancer treatment (group B)...
October 2017: Reproductive Medicine and Biology
Dora Reglodi, Sandor Cseh, Bence Somoskoi, Balazs Fulop, Eszter Szentleleky, Vince Szegeczki, Anna Kovacs, Andrea Varga, Peter Kiss, Hitoshi Hashimoto, Andrea Tamas, Attila Bardosi, Sridharan Manavalan, Eva Bako, Roza Zakany, Tamas Juhasz
PACAP is a neuropeptide with diverse functions in various organs, including reproductive system. It is present in the testis in high concentrations, and in addition to the stage-specific expression within the seminiferous tubules, PACAP affects spermatogenesis and the functions of Leydig and Sertoli cells. Mice lacking endogenous PACAP show reduced fertility, but the possibility of abnormalities in spermatogenic signaling has not yet been investigated. Therefore, we performed a detailed morphological analysis of spermatozoa, sperm motility, and investigated signaling pathways that play a role during spermatogenesis in knockout mice...
November 3, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
Takayuki Yamochi, Shu Hashimoto, Masaya Yamanaka, Yoshiharu Nakaoka, Yoshiharu Morimoto
To determine the optimum culture duration for porcine growing oocytes (GOs) to attain maturation competence, we examined the meiotic competence, chromatin configuration, and fertilization ability of porcine oocytes obtained from early antral follicles and cultured for 10-16 days. The survival rate of oocytes after 10 days of culture (62.8%) was similar to that of oocytes after 12 days of culture (55%) and significantly higher than that of oocytes cultured for 14 and 16 days (52.9% and 24.3%, respectively). No significant difference was observed in the diameter of ooplasm from oocytes cultured for different durations (117...
October 12, 2017: Journal of Reproduction and Development
K Kyono, T Hashimoto, M Toya, M Koizumi, C Sasaki, S Shibasaki, N Aono, Y Nakamura, R Obata, N Okuyama, Y Ogura, H Igarashi
PURPOSE: The purpose of this study was to examine the efficacy of an ovarian tissue transportation network for fertility preservation (FP) for cancer patients in Japan. METHODS: PubMed was searched for papers on transportation of human ovarian tissue for FP. We analyzed population, area, number of cancer patients for ovarian tissue cryopreservation (OTC), quality control/assessment and safety, cost of a cryopreservation center for the building for 30 years, and medical fees of cancer patients (operation, cryopreservation, and storage of ovarian tissue)...
November 2017: Journal of Assisted Reproduction and Genetics
Mika Ishigaki, Kosuke Hashimoto, Hidetoshi Sato, Yukihiro Ozaki
Current research focuses on embryonic development and quality not only by considering fundamental biology, but also by aiming to improve assisted reproduction technologies, such as in vitro fertilization. In this study, we explored the development of mouse embryo and its quality based on molecular information, obtained nondestructively using Raman spectroscopy. The detailed analysis of Raman spectra measured in situ during embryonic development revealed a temporary increase in protein content after fertilization...
March 8, 2017: Scientific Reports
K Kowalczyk, G Franik, D Kowalczyk, D Pluta, Ł Blukacz, P Madej
OBJECTIVE: Thyroid disorders, especially Hashimoto's thyroiditis (HT), are observed significantly more often in patients with polycystic ovary syndrome (PCOS) than in the general population - approximately 27% and 8%, respectively. This is extremely important in young women, because both disorders are connected with fertility problems. As HT and PCOS occur together, fertility problems may become a serious clinical issue in these patients. MATERIALS AND METHODS: A systematic literature review in PubMed of PCOS- and HT-related articles in English, published until December 2015 was conducted...
January 2017: European Review for Medical and Pharmacological Sciences
Sonoko Shimoji, Daigo Hashimoto, Hidetsugu Tsujigiwa, Kohta Miyawaki, Koji Kato, Shuichiro Takahashi, Reiki Ogasawara, Takashi Jiromaru, Hiromi Iwasaki, Toshihiro Miyamoto, Koichi Akashi, Takanori Teshima
Infertility associated with ovarian failure is a serious late complication for female survivors of allogeneic hematopoietic stem cell transplantation (SCT). Although pretransplant conditioning regimen has been appreciated as a cause of ovarian failure, increased application of reduced-intensity conditioning allowed us to revisit other factors possibly affecting ovarian function after allogeneic SCT. We have addressed whether donor T-cell-mediated graft-versus-host disease (GVHD) could be causally related to female infertility in mice...
March 2, 2017: Blood
Fuminori Tanihara, Tatsuya Takemoto, Eri Kitagawa, Shengbin Rao, Lanh Thi Kim Do, Akira Onishi, Yukiko Yamashita, Chisato Kosugi, Hitomi Suzuki, Shoichiro Sembon, Shunichi Suzuki, Michiko Nakai, Masakazu Hashimoto, Akihiro Yasue, Munehide Matsuhisa, Sumihare Noji, Tatsuya Fujimura, Dai-Ichiro Fuchimoto, Takeshige Otoi
Genetically modified pigs for biomedical applications have been mainly generated using the somatic cell nuclear transfer technique; however, this approach requires complex micromanipulation techniques and sometimes increases the risks of both prenatal and postnatal death by faulty epigenetic reprogramming of a donor somatic cell nucleus. As a result, the production of genetically modified pigs has not been widely applied. We provide a simple method for CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 gene editing in pigs that involves the introduction of Cas9 protein and single-guide RNA into in vitro fertilized zygotes by electroporation...
September 2016: Science Advances
Masaya Yamanaka, Kazuhisa Tomita, Shu Hashimoto, Hiroshi Matsumoto, Manabu Satoh, Hiromi Kato, Yoshihiko Hosoi, Masayasu Inoue, Yoshiharu Nakaoka, Yoshiharu Morimoto
Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive techniques in humans. We investigated whether semen preparation using a combination of DGC and swim-up techniques could effectively decrease morphologically abnormal human sperms at the ultrastructural level. Semen samples were obtained from 16 infertile males and fractionated by swim-up following DGC. Ultrastructural abnormalities of sperms obtained from original semen, lower layer of swim-up following DGC, and upper layer of swim-up following DGC were analyzed by transmission electron microscopy...
December 20, 2016: Journal of Reproduction and Development
Masakazu Hashimoto, Yukiko Yamashita, Tatsuya Takemoto
The CRISPR/Cas9 system is a powerful tool for elucidating the roles of genes in a wide variety of organisms including mice. To obtain genetically modified embryos or mice by this method, Cas9 mRNA and sgRNA are usually introduced into zygotes by microinjection or electroporation. However, most mutants generated with this method are genetically mosaic, composed of several types of cells carrying different mutations, which complicates phenotype analysis in founder embryos or mice. To simplify the analysis and to elucidate the roles of genes involved in developmental processes, a method for producing non-mosaic mutants is needed...
October 1, 2016: Developmental Biology
Koji Koizumi, Toru Fujioka, Toshiaki Yasuoka, Aya Inoue, Yuka Uchikura, Hiroki Tanaka, Katsuko Takagi, Miki Mori, Masae Koizumi, Hisashi Hashimoto, Takashi Matsumoto, Yuko Matsubara, Keiichi Matsubara, Akihiro Nawa
Multiple techniques have been used for the conservative treatment of high-grade cervical intraepithelial neoplasia (HG-CIN) in women of fertile age. Conization has been associated with stenosis of the cervix and a decrease in cervical mucus secretion, in addition to the increase in the risk of cervical canal shortening and problems during the perinatal period, including premature birth and premature rupture of membranes. Although the laser transpiration technique does not cause shortening of the cervical canal, it is associated with the recurrent risk of deep residual disease...
August 2016: Molecular and Clinical Oncology
Yuki Takahashi, Shu Hashimoto, Takayuki Yamochi, Hiroya Goto, Masaya Yamanaka, Ami Amo, Hiroshi Matsumoto, Masayasu Inoue, Keijiro Ito, Yoshiharu Nakaoka, Nao Suzuki, Yoshiharu Morimoto
PURPOSE: The change of mitochondrial distribution in human oocytes during meiotic maturation was assessed using 223 human oocytes donated from patients undergoing fertility treatment between June 2013 and February 2016. METHODS: Live cell images of fluorescence-labelled mitochondria in human oocytes were analysed to investigate dynamic changes in mitochondrial distribution during meiotic maturation using a confocal microscope combined with an incubator in the presence or absence of colchicine and cytochalasin B, inhibitors for tubulin and actin filament, respectively...
July 2016: Journal of Assisted Reproduction and Genetics
Shu Hashimoto, Tatsuya Nakano, Kazuo Yamagata, Masayasu Inoue, Yoshiharu Morimoto, Yoshiharu Nakaoka
OBJECTIVE: To assess the developmental competence of human embryos with multinucleation (MN). DESIGN: Experimental study. SETTING: Private fertility center. PATIENT(S): Forty-four couples donating 143 zygotes for confocal imaging study, and 78 couples included in the retrospective clinical study. INTERVENTION(S): Time-lapse imaging study using confocal and light microscopes. MAIN OUTCOME MEASURE(S): Cytokinesis at first mitosis, MN, chromosomal behavior, euploidy, implantation, successful delivery of healthy baby...
July 2016: Fertility and Sterility
Matthew W Pitts, Penny M Kremer, Ann C Hashimoto, Daniel J Torres, China N Byrns, Christopher S Williams, Marla J Berry
UNLABELLED: Selenium (Se) is essential for both brain development and male fertility. Male mice lacking two key genes involved in Se metabolism (Scly(-/-)Sepp1(-/-) mice), selenoprotein P (Sepp1) and Sec lyase (Scly), develop severe neurological dysfunction, neurodegeneration, and audiogenic seizures that manifest beginning in early adulthood. We demonstrate that prepubescent castration of Scly(-/-)Sepp1(-/-) mice prevents behavioral deficits, attenuates neurodegeneration, rescues maturation of GABAergic inhibition, and increases brain selenoprotein levels...
November 18, 2015: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Noriko Ouji-Sageshima, Katsuya Yuui, Mari Nakanishi, Nobuyoshi Takeda, Yasushi Odawara, Masanori Yamashita, Hiroshi Iwayama, Keiko Awai, Hirotsugu Hashimoto, Daniel E Geraghty, Akiko Ishitani, Katsuhiko Hatake, Toshihiro Ito
In the field of in vitro fertilization (IVF), useful markers for the prediction of successful implantation for oocyte or embryo selection are essential. It has been reported that sHLA-G (sHLA-G1/HLA-G5) could be detected in the supernatant of the fertilized embryo and in follicular fluid samples (FFs), and that the presence of sHLA-G was related to successful implantation. If sHLA-G could be used as a marker of oocyte selection from multiple FFs, oocytes could be selected without physical contact, thus reducing the likelihood of damage...
February 2016: Journal of Reproductive Immunology
T Yamochi, S Hashimoto, A Amo, H Goto, M Yamanaka, M Inoue, Y Nakaoka, Y Morimoto
Meiotic maturation of oocytes requires a variety of ATP-dependent reactions, such as germinal vesicle breakdown, spindle formation, and rearrangement of plasma membrane structure, which is required for fertilization. Mitochondria are accordingly expected be localized to subcellular sites of energy utilization. Although microtubule-dependent cellular traffic for mitochondria has been studied extensively in cultured neuronal (and some other somatic) cells, the molecular mechanism of their dynamics in mammalian oocytes at different stages of maturation remains obscure...
August 2016: Zygote: the Biology of Gametes and Early Embryos
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