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https://www.readbyqxmd.com/read/29235576/the-unfolded-protein-response-impacts-melanoma-progression-by-enhancing-fgf-expression-and-can-be-antagonized-by-a-chemical-chaperone
#1
Karin Eigner, Yüksel Filik, Florian Mark, Birgit Schütz, Günter Klambauer, Richard Moriggl, Markus Hengstschläger, Herbert Stangl, Mario Mikula, Clemens Röhrl
The mechanisms hallmarking melanoma progression are insufficiently understood. Here we studied the impact of the unfolded protein response (UPR) - a signalling cascade playing ambiguous roles in carcinogenesis - in melanoma malignancy. We identified isogenic patient-derived melanoma cell lines harboring BRAFV600E-mutations as a model system to study the role of intrinsic UPR in melanoma progression. We show that the activity of the three effector pathways of the UPR (ATF6, PERK and IRE1) was increased in metastatic compared to non-metastatic cells...
December 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29133075/spinal-cord-self-repair-during-tail-regeneration-in-polypedates-maculatus-and-putative-role-of-fgf1-as-a-neurotrophic-factor
#2
Jutshina Hota, Sushri Sangita Pati, Pravati Kumari Mahapatra
Spinal cord injury could be fatal in man and often results in irreversible medical conditions affecting mobility. However, anuran amphibians win over such pathological condition by the virtue of regeneration abilities. The tail of anuran tadpoles therefore allures researchers to study spinal cord injury and self- repair process. In the present study, we inflicted injury to the spinal cord by means of surgical transection of the tail and investigated the self-repair activity in the tadpoles of the Indian tree frog Polypedates maculatus...
November 10, 2017: Journal of Chemical Neuroanatomy
https://www.readbyqxmd.com/read/29128220/identification-of-potential-embryokines-in-the-bovine-reproductive-tract
#3
P Tríbulo, L G B Siqueira, L J Oliveira, T Scheffler, P J Hansen
Knowledge of the molecules used by the maternal reproductive tract to regulate development of the preimplantation embryo is largely incomplete. The goal of the present experiment was to identify candidates for this function. The approach was to assess expression patterns in the endometrium and oviduct of 93 genes encoding for hormones, growth factors, chemokines, cytokines, and WNT-related molecules. Results show that all of the genes were expressed in the reproductive tract. Expression in oviduct was affected by day of the estrous cycle for 21 genes with 11 genes having highest expression at estrus (CCL21, CTGF, CXCL10, CXCL16, DKK3, FGF10, IL18, IL33, IL34, PGF, and SFRP2), one gene at d 3 (WNT4), 8 at d 5 (BMP7, HGF, IL6, SFRP1, TGFB1, WIF1, WNT2, and WNT5A), and one at d 7 (IK)...
November 8, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29117903/histopathological-and-immunohistochemical-analysis-of-the-use-of-collagen-dressing-as-a-reinforcement-of-esophagic-anastomosis-in-a-rat-experimental-model
#4
Rocio Garcia-Perez, Vicente Munitiz, Carlos Manuel Martinez-Caceres, David Ruiz de Angulo, Angeles Ortiz, Luisa F Martinez de Haro, Diana Navas, Pascual Parrilla
INTRODUCTION: One of the most severe complications after esophaguectomy is anastomotic dehiscence. The use of collagen sponges could be an effective way to resolve this complication. Our objective was to perform an experimental model of esophageal anastomosis in rats to study these mechanisms. METHODS: A total of 50 Sprague-Dawley rats were used divided into 2 groups, Tachosil(®) group (n=25) and control group (n=25). After the section of the abdominal esophagus a single-layer esophago-gastric anastomosis was performed reinforced with 1cm of Tachosil(®) wrapping the anastomosis in group 1...
November 5, 2017: Cirugía Española
https://www.readbyqxmd.com/read/29091276/simple-and-efficient-purification-of-recombinant-proteins-using-the-heparin-binding-affinity-tag
#5
Srinivas Jayanthi, Ravi Kumar Gundampati, Thallapuranam Krishnaswamy Suresh Kumar
Heparin, a member of the glycosaminoglycan family, is known to interact with more than 400 different types of proteins. For the past few decades, significant progress has been made to understand the molecular details involved in heparin-protein interactions. Based on the structural knowledge available from the FGF1-heparin interaction studies, we have designed a novel heparin-binding peptide (HBP) affinity tag that can be used for the simple, efficient, and cost-effective purification of recombinant proteins of interest...
November 1, 2017: Current Protocols in Protein Science
https://www.readbyqxmd.com/read/29048426/fgf1-protects-neuroblastoma-sh-sy5y-cells-from-p53-dependent-apoptosis-through-an-intracrine-pathway-regulated-by-fgf1-phosphorylation
#6
Caroline Pirou, Fatemeh Montazer-Torbati, Nadège Jah, Elisabeth Delmas, Christelle Lasbleiz, Bernard Mignotte, Flore Renaud
Neuroblastoma, a sympathetic nervous system tumor, accounts for 15% of cancer deaths in children. In contrast to most human tumors, p53 is rarely mutated in human primary neuroblastoma, suggesting impaired p53 activation in neuroblastoma. Various studies have shown correlations between fgf1 expression levels and both prognosis severity and tumor chemoresistance. As we previously showed that fibroblast growth factor 1 (FGF1) inhibited p53-dependent apoptosis in neuron-like PC12 cells, we initiated the study of the interaction between the FGF1 and p53 pathways in neuroblastoma...
August 31, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29017078/fgf1-and-igf1-conditioned-3d-culture-system-promoted-the-amplification-and-cancer-stemness-of-lung-cancer-cells
#7
Pengpeng Liu, Rui Zhang, Wenwen Yu, Yingnan Ye, Yanan Cheng, Lei Han, Li Dong, Yongzi Chen, Xiyin Wei, Jinpu Yu
Lung cancer stem cells (LCSCs) are considered as the cellular origins of metastasis and relapse of lung cancer. However, routine two-dimensional culture system (2D-culture) hardly mimics the growth and functions of LCSCs in vivo and therefore significantly decreases the stemness activity of LCSCs. In this study, we constructed a special BME-based three-dimensional culture system (3D-culture) to amplify LCSCs in human lung adenocarcinoma cell line A549 cells and found 3D-culture promoted the enrichment and amplification of LCSCs in A549 cells displaying higher proliferation potential and invasion activity, but lower apoptosis...
September 28, 2017: Biomaterials
https://www.readbyqxmd.com/read/29016740/a-novel-fibroblast-growth-factor-1-ligand-with-reduced-heparin-binding-protects-the-heart-against-ischemia-reperfusion-injury-in-the-presence-of-heparin-co-administration
#8
Chahua Huang, Yang Liu, Andrew Beenken, Lin Jiang, Xiang Gao, Zhifeng Huang, Anna Hsu, Garrett J Gross, Yi-Gang Wang, Moosa Mohammadi, Jo El J Schultz
Aims: Fibroblast growth factor 1 (FGF1), a heparin/heparan sulfate-binding growth factor, is a potent cardioprotective agent against myocardial infarction (MI). The impact of heparin, the standard of care for MI patients entering the emergency room, on cardioprotective effects of FGF1 is unknown, however. Methods and results: To address this, a rat model of MI was employed to compare cardioprotective potentials (lower infarct size and improve post-ischemic function) of native FGF1 and an engineered FGF1 (FGF1ΔHBS) with reduced heparin-binding affinity when given at the onset of reperfusion in the absence or presence of heparin...
November 1, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28949091/new-approach-for-the-treatment-of-neuropathic-pain-fibroblast-growth-factor-1-gene-transfected-adipose-derived-mesenchymal-stem-cells
#9
F Forouzanfar, B Amin, A Ghorbani, H Ghazavi, F Ghasemi, K Sadri, S Mehri, H R Sadeghnia, H Hosseinzadeh
BACKGROUND: Neuropathic pain triggered by peripheral nerve lesion is extremely difficult to manage with current approaches, hence the importance of exploring therapeutic alternatives. METHODS: We have analysed adipose-derived mesenchymal stem cells (AD-MSCs) and fibroblast growth factor 1 gene-transfected adipose-derived mesenchymal stem cells (AD-MSCs FGF1 ) on chronic constriction injury (CCI). The mechanical and thermal hypersensitivity were assessed using the von Frey filament, radiant heat and acetone drop tests...
September 26, 2017: European Journal of Pain: EJP
https://www.readbyqxmd.com/read/28947720/identification-of-genes-involved-in-cold-shock-response-in-rainbow-trout-oncorhynchus-mykiss
#10
Andreas Borchel, Marieke Verleih, Alexander Rebl, Tom Goldammer
A rapid decline in temperature poses a major challenge for poikilothermic fish, as their entire metabolism depends on ambient temperature. The gene expression of rainbow trout Oncorhynchus mykiss having undergone such a cold shock (0◦C) was compared to a control (5◦C) in a microarray and quantitative real-time PCR based study. The tissues of gill, kidney and liver were examined. The most differently expressed genes were found in liver, many of them contributing to the network 'cellular compromise, cellular growth and proliferation'...
September 2017: Journal of Genetics
https://www.readbyqxmd.com/read/28941572/effects-of-neural-stem-cell-media-on-hypoxic-injury-in-rat-hippocampal-slice-cultures
#11
Na Mi Lee, Soo Ahn Chae, Hong Jun Lee
Neonatal hypoxic-ischemic brain injuries cause serious neurological sequelae, yet there is currently no effective treatment for them. We hypothesized that neurotrophic factors released into the medium by stem cells could supply hypoxia-damaged organotypic hippocampal slice cultures with regenerative abilities. We prepared organotypic slice cultures of the hippocampus of 7-day-old Sprague-Dawley rats based on the modified Stoppini method; slices were cultured for 14days in vitro using either Gahwiler's medium (G-medium) or stem cell-conditioned medium (S-medium) as culture medium...
December 15, 2017: Brain Research
https://www.readbyqxmd.com/read/28922558/biomechanical-properties-and-healing-effects-of-chitin-patch-in-a-rat-full-thickness-abdominal-wall-defect-model
#12
Jing Qiao, Zhiwen Jiang, Xuyue Liang, Yan Yang, Wanshun Liu, Baoqin Han
Hernia repair is usually accompanied with the implantation of a synthetic mesh, which frequently results in a foreign body response and serious complications. In the present study, a novel biodegradable chitin-based hernia patch was prepared and characterized. Biomechanical properties and biodegradability of the chitin patch were quantified in vitro and in vivo. In repair of the rat abdominal wall full-thickness defect model, the chitin patch induced more abundant new blood vessels with milder tissue inflammation and fibrosis compared with polypropylene mesh...
September 18, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28881626/relevance-of-ccl3-ccr5-axis-in-oral-carcinogenesis
#13
Janine Mayra da Silva, Tálita Pollyanna Moreira Dos Santos, Lays Martin Sobral, Celso Martins Queiroz-Junior, Milene Alvarenga Rachid, Amanda E I Proudfoot, Gustavo Pompermaier Garlet, Aline Carvalho Batista, Mauro Martins Teixeira, Andréia Machado Leopoldino, Remo Castro Russo, Tarcília Aparecida Silva
The chemokine CCL3 is a chemotactic cytokine crucial for inflammatory cell recruitment in homeostatic and pathological conditions. CCL3 might stimulate cancer progression by promoting leukocyte accumulation, angiogenesis and tumour growth. The expression of CCL3 and its receptors CCR1 and CCR5 was demonstrated in oral squamous cell carcinoma (OSCC), but their role was not defined. Here, the functions of CCL3 were assessed using a model of chemically induced tongue carcinogenesis with 4-nitroquinoline-1-oxide (4NQO)...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28813681/uncoupling-the-mitogenic-and-metabolic-functions-of-fgf1-by-tuning-fgf1-fgf-receptor-dimer-stability
#14
Zhifeng Huang, Yi Tan, Junlian Gu, Yang Liu, Lintao Song, Jianlou Niu, Longwei Zhao, Lakshmi Srinivasan, Qian Lin, Jingjing Deng, Yang Li, Daniel J Conklin, Thomas A Neubert, Lu Cai, Xiaokun Li, Moosa Mohammadi
The recent discovery of metabolic roles for fibroblast growth factor 1 (FGF1) in glucose homeostasis has expanded the functions of this classically known mitogen. To dissect the molecular basis for this functional pleiotropy, we engineered an FGF1 partial agonist carrying triple mutations (FGF1(ΔHBS)) that diminished its ability to induce heparan sulfate (HS)-assisted FGF receptor (FGFR) dimerization and activation. FGF1(ΔHBS) exhibited a severely reduced proliferative potential, while preserving the full metabolic activity of wild-type FGF1 in vitro and in vivo...
August 15, 2017: Cell Reports
https://www.readbyqxmd.com/read/28795363/fibroblast-growth-factor-type-1-fgf1-overexpressed-adipose-derived-mesenchaymal-stem-cells-ad-msc-fgf1-induce-neuroprotection-and-functional-recovery-in-a-rat-stroke-model
#15
Hamed Ghazavi, Seyed Javad Hoseini, Alireza Ebrahimzadeh-Bideskan, Baratali Mashkani, Soghra Mehri, Ahmad Ghorbani, Kayvan Sadri, Elahe Mahdipour, Faezeh Ghasemi, Fatemeh Forouzanfar, Azar Hoseini, Ali Reza Pasdar, Hamid Reza Sadeghnia, Majid Ghayour-Mobarhan
Stroke, as the second most common cause of death, imposes a great financial burden on both the individual and society. Mesenchymal stem cells from rodents have demonstrated efficacy in experimental animal models of stroke due to enhanced neurological recovery. Since FGF1 (fibroblast growth factor 1) displays neuroprotective properties, for the first time, we investigated the effect of acute intravenous administration of FGF1 gene transfected adipose-derived mesenchymal stem cell (AD-MSC(FGF1)) on transient experimental ischemic stroke in rats...
October 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28733346/regulation-and-action-of-early-growth-response-1-in-bovine-granulosa-cells
#16
Peng Han, Hilda Guerrero-Netro, Anthony Estienne, Binyun Cao, Christopher Price
Fibroblast growth factors (FGF) modify cell proliferation and differentiation through receptor tyrosine kinases, which stimulate the expression of transcription factors including members of the early growth response (EGR) family. In ovarian granulosa cells, most FGFs activate typical response genes, although the role of EGR proteins has not been described. In the present study, we determined the regulation of EGR mRNA by FGFs and explored the role of EGR1 in the regulation of FGF response genes. Addition of FGF1, FGF2, FGF4 or FGF8b increased EGR1 and EGR3 mRNA levels, whereas FGF18 increased only EGR1 mRNA abundance...
July 21, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28674664/recombinant-expression-in-e-coli-of-human-fgfr2-with-its-transmembrane-and-extracellular-domains
#17
Adam Bajinting, Ho Leung Ng
Fibroblast growth factor receptors (FGFRs) are a family of receptor tyrosine kinases containing three domains: an extracellular receptor domain, a single transmembrane helix, and an intracellular tyrosine kinase domain. FGFRs are activated by fibroblast growth factors (FGFs) as part of complex signal transduction cascades regulating angiogenesis, skeletal formation, cell differentiation, proliferation, cell survival, and cancer. We have developed the first recombinant expression system in E. coli to produce a construct of human FGFR2 containing its transmembrane and extracellular receptor domains...
2017: PeerJ
https://www.readbyqxmd.com/read/28664920/fgf1-a-new-weapon-to-control-type-2-diabetes-mellitus
#18
REVIEW
Emanuel Gasser, Christopher P Moutos, Michael Downes, Ronald M Evans
A hypercaloric diet combined with a sedentary lifestyle is a major risk factor for the development of insulin resistance, type 2 diabetes mellitus (T2DM) and associated comorbidities. Standard treatment for T2DM begins with lifestyle modification, and includes oral medications and insulin therapy to compensate for progressive β-cell failure. However, current pharmaceutical options for T2DM are limited in that they do not maintain stable, durable glucose control without the need for treatment intensification...
October 2017: Nature Reviews. Endocrinology
https://www.readbyqxmd.com/read/28656229/co-expression-of-podoplanin-and-fibroblast-growth-factor-1-predicts-poor-prognosis-in-patients-with-lung-squamous-cell-carcinoma
#19
Juan Li, Han Chen, Xiaoqing Li, Linlin Wang, Aiqin Gao, Pei Zhang, Wenli Lin, Wei Gao, Dong Yang, Xiaosun Guo, Jie Liu, Qi Dang, Yuping Sun
Podoplanin and fibroblast growth factor (FGF) 1 have been detected more frequently in lung squamous cell carcinoma (SQCC) compared with lung adenocarcinoma. Furthermore, it has been previous demonstrated that FGF1 is located on the edge of tumor nests in certain lung SQCC sections, which resembles the characteristic expression pattern of podoplanin. Podoplanin and FGF1 have roles in lymphangiogenesis and angiogenesis. Based on their consistently specific expression in lung SQCC and similar localization patterns, the present study aimed to investigate whether the expression of podoplanin in tumor cells is correlated with FGF1 expression in lung SQCC and whether their co‑expression has clinicopathological significance, particularly for lymphangiogenesis/angiogenesis...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28655615/lncrna-map2k4-sequesters-mir-199a-to-promote-fgf1-expression-and-spinal-cord-neuron-growth
#20
Hao-Ran Lv
Spinal cord injury (SCI) is a common critical illness in clinical practice. SCI prevention, treatment and rehabilitation have become important topics in today's medical profession. Studies have shown that long noncoding RNAs (lncRNAs) also play an important role in the pathology of SCI. The biology software analysis identified miR-199a binding sites in the lncRNA-Map2k4 and FGF1 sequences, which were confirmed by the subsequent dual luciferase reporter assay. When lncRNA-Map2k4 expression was down-regulated by siRNA, miR-199a expression in neurons was up-regulated and FGF1 expression was down-regulated...
August 26, 2017: Biochemical and Biophysical Research Communications
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