keyword
MENU ▼
Read by QxMD icon Read
search

prmt1

keyword
https://www.readbyqxmd.com/read/28057797/prmt2-interacts-with-splicing-factors-and-regulates-the-alternative-splicing-of-bcl-x
#1
Mynol I Vhuiyan, Magnolia L Pak, Margaret A Park, Dylan Thomas, Ted M Lakowski, Charles E Chalfant, Adam Frankel
Protein arginine N-methyltransferase 2 (PRMT2) functions in JAK-STAT and Wnt/β-catenin signaling pathways, serves as a nuclear receptor-dependent transcriptional co-activator, and represses NF-κB and E2F1 transcription factor activities to promote apoptosis. We have previously demonstrated that PRMT2 interacts with PRMT1 and increases its activity. Here, we reveal associations using proteomics between the PRMT2 SH3 domain and splicing factors including Src-associated in mitosis 68 kDa protein (SAM68), a PRMT1 substrate and trans-acting factor that mediates BCL-X alternative splicing...
January 5, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28040436/fam98a-associates-with-ddx1-c14orf166-fam98b-in-a-novel-complex-involved-in-colorectal-cancer-progression
#2
Khondker Ayesha Akter, Mohammed A Mansour, Toshinori Hyodo, Takeshi Senga
Protein Arginine Methyl Transferase 1 (PRMT1) is deemed to be a potential oncogenic protein considering its overexpression in several malignancies including colorectal cancer. However, the molecular pathogenesis regarding PRMT1 overexpression and overall poor patient survival involved in this devastating and life threatening cancer remains obscured. In our previous study, we have identified FAM98A as a novel substrate of PRMT1 and also identified its role in ovarian cancer progression. Here, we showed that the two structural homologs FAM98A and FAM98B included in a novel complex with DDX1 and C14orf166 are required for PRMT1 expression...
December 28, 2016: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28003433/angiodysplasia-in-embryo-lacking-protein-arginine-methyltransferase-1-prmt1-in-vascular-endothelial-cells
#3
Tomohiro Ishimaru, Junji Ishida, Jun-Dal Kim, Hayase Mizukami, Kanako Hara, Misuzu Hashimoto, Ken-Ichi Yagami, Fumihiro Sugiyama, Akiyoshi Fukamizu
Protein arginine methyltransferase 1 (PRMT1) is involved in multiple cellular functions including proliferation and differentiation. Although PRMT1 is expressed in vascular endothelial cells (ECs), which are responsible for angiogenesis during embryonic development, its role has remained elusive. In this study, we generated endothelial-specific prmt1-knockout (Prmt1-ECKO) mice, and found that they died before embryonic day 15. The superficial temporal arteries in these embryos were poorly perfused with blood, and whole-mount 3D imaging revealed dilated and segmentalized luminal structures in Prmt1-ECKO fetuses in comparison with those of controls...
December 21, 2016: Journal of Biochemistry
https://www.readbyqxmd.com/read/27994012/asymmetric-arginine-dimethylation-modulates-mitochondrial-energy-metabolism-and-homeostasis-in-caenorhabditis-elegans
#4
Liang Sha, Hiroaki Daitoku, Sho Araoi, Yuta Kaneko, Yuta Takahashi, Koichiro Kako, Akiyoshi Fukamizu
Protein Arginine Methyltransferase 1 (PRMT1) catalyzes asymmetric arginine dimethylation on cellular proteins and modulates various aspects of biological processes, such as signal transduction, DNA repair, and transcriptional regulation. We have previously reported that the null mutant of prmt-1 in C. elegans exhibits a slightly short lifespan, but the physiological significances of PRMT-1 remains largely unclear. Here we explored the role of PRMT-1 on mitochondrial function as hinted by a two-dimensional Western blot-based proteomic study...
December 19, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27957828/protective-effects-of-low-dose-rosuvastatin-on-isoproterenol-induced-chronic-heart-failure-in-rats-by-regulation-of-ddah-adma-no-pathway
#5
Ru Zhou, Ping Ma, Aiqin Xiong, Yehua Xu, Yang Wang, Qingbin Xu
Cardiovascular disease is the leading cause of death with high morbidity and mortality and chronic heart failure is the terminal phase of it. This study aims to investigate the protective effects of the low dose rosuvastatin on isoproterenol-induced chronic heart failure and to explore the possible related mechanisms METHODS: Male Sprague-Dawley rats were given isoproterenol 5 mg/kg once a day for 7 days to establish heart failure model by subcutaneous injection. Simultaneously, low dose rosuvastatin (5 mg/kg) was oral administrated from day 1 to day 14...
December 13, 2016: Cardiovascular Therapeutics
https://www.readbyqxmd.com/read/27876471/cobalamin-and-folate-protect-mitochondrial-and-contractile-functions-in-a-murine-model-of-cardiac-pressure-overload
#6
Jérôme Piquereau, Maryline Moulin, Giada Zurlo, Philippe Mateo, Mélanie Gressette, Jean-Louis Paul, Christophe Lemaire, Renée Ventura-Clapier, Vladimir Veksler, Anne Garnier
PGC-1α, a key regulator of energy metabolism, seems to be a relevant therapeutic target to rectify the energy deficit observed in heart failure (HF). Since our previous work has shown positive effects of cobalamin (Cb) on PGC-1α cascade, we investigate the protective role of Cb in pressure overload-induced myocardial dysfunction. Mice were fed with normal diet (ND) or with Cb and folate supplemented diet (SD) 3weeks before and 4weeks after transverse aortic constriction (TAC). At the end, left ventricle hypertrophy and drop of ejection fraction were significantly lower in SD mice than in ND mice...
November 20, 2016: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/27849571/arginine-methylation-by-prmt1-regulates-muscle-stem-cell-fate
#7
Roméo Sébastien Blanc, Gillian Vogel, Xing Li, Zhenbao Yu, Shawn Li, Stéphane Richard
Quiescent muscle stem cells (MSC) become activated in response to skeletal muscle injury to initiate regeneration. Activated MSCs proliferate and differentiate to repair damaged fibers or self-renew to maintain the pool and insure future regeneration. The balance between self-renewal, proliferation and differentiation is a tightly regulated process controlled by a genetic cascade involving determinant transcription factors such as Pax7, Myf5, MyoD, and MyoG. Recently there have been several reports about the role of arginine methylation as a requirement for epigenetic-mediated control of muscle regeneration...
November 14, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27834681/transient-kinetics-define-a-complete-kinetic-model-for-protein-arginine-methyltransferase-1
#8
Hao Hu, Cheng Luo, Y George Zheng
Protein arginine methyltransferases (PRMTs) are the enzymes responsible for posttranslational methylation of protein arginine residues in eukaryotic cells, particularly within the histone tails. A detailed mechanistic model of PRMT-catalyzed methylation is currently lacking, but it is essential for understanding the functions of PRMTs in various cellular pathways and for efficient design of PRMT inhibitors as potential treatments for a range of human diseases. In this work, we used stopped-flow fluorescence in combination with global kinetic simulation to dissect the transient kinetics of PRMT1, the predominant type I arginine methyltransferase...
December 23, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27793800/gene-expression-profiling-of-epigenetic-chromatin-modification-enzymes-and-histone-marks-by-cigarette-smoke-implications-for-copd-and-lung-cancer
#9
Isaac K Sundar, Irfan Rahman
Chromatin-modifying enzymes mediate DNA methylation and histone modifications on recruitment to specific target gene loci in response to various stimuli. The key enzymes that regulate chromatin accessibility for maintenance of modifications in DNA and histones, and for modulation of gene expression patterns in response to cigarette smoke (CS), are not known. We hypothesize that CS exposure alters the gene expression patterns of chromatin-modifying enzymes, which then affects multiple downstream pathways involved in the response to CS...
December 1, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27758883/oncogenic-functions-of-gli1-in-pancreatic-adenocarcinoma-are-supported-by-its-prmt1-mediated-methylation
#10
Yan Wang, Jung-Mao Hsu, Ya'an Kang, Yongkun Wei, Pei-Chih Lee, Shing-Jyh Chang, Yi-Hsin Hsu, Jennifer L Hsu, Hung-Ling Wang, Wei-Chao Chang, Chia-Wei Li, Hsin-Wei Liao, Shih-Shin Chang, Weiya Xia, How-Wen Ko, Chao-Kai Chou, Jason B Fleming, Huamin Wang, Rosa F Hwang, Yue Chen, Jun Qin, Mien-Chie Hung
The oncogenic transcription factor Gli1 is a critical effector in the Hedgehog (Hh) pathway, which is necessary for the development and progression of pancreatic ductal adenocarcinoma (PDAC). Although TGFβ and K-Ras are known regulators of Gli1 gene transcription in this setting, it is not understood how Gli1 functional activity is regulated. Here, we report the identification of Gli1 as a substrate for the protein arginine N-methyltransferase PRMT1 in PDAC. We found that PRMT1 methylates Gli1 at R597, promoting its transcriptional activity by enhancing the binding of Gli1 to its target gene promoters...
December 1, 2016: Cancer Research
https://www.readbyqxmd.com/read/27683223/chtop-chromatin-target-of-prmt1-auto-regulates-its-expression-level-via-intron-retention-and-nonsense-mediated-decay-of-its-own-mrna
#11
Keiichi Izumikawa, Harunori Yoshikawa, Hideaki Ishikawa, Yuko Nobe, Yoshio Yamauchi, Sjaak Philipsen, Richard J Simpson, Toshiaki Isobe, Nobuhiro Takahashi
Chtop (chromatin target of Prmt1) regulates various aspects of gene expression including transcription and mRNA export. Despite these important functions, the regulatory mechanism underlying Chtop expression remains undetermined. Using Chtop-expressing human cell lines, we demonstrate that Chtop expression is controlled via an autoregulatory negative feedback loop whereby Chtop binds its own mRNA to retain intron 2 during splicing; a premature termination codon present at the 5' end of intron 2 leads to nonsense-mediated decay of the mRNA...
November 16, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27642082/prmt1-mediated-methylation-of-micu1-determines-the-ucp2-3-dependency-of-mitochondrial-ca-2-uptake-in-immortalized-cells
#12
Corina T Madreiter-Sokolowski, Christiane Klec, Warisara Parichatikanond, Sarah Stryeck, Benjamin Gottschalk, Sergio Pulido, Rene Rost, Emrah Eroglu, Nicole A Hofmann, Alexander I Bondarenko, Tobias Madl, Markus Waldeck-Weiermair, Roland Malli, Wolfgang F Graier
Recent studies revealed that mitochondrial Ca(2+) channels, which control energy flow, cell signalling and death, are macromolecular complexes that basically consist of the pore-forming mitochondrial Ca(2+) uniporter (MCU) protein, the essential MCU regulator (EMRE), and the mitochondrial Ca(2+) uptake 1 (MICU1). MICU1 is a regulatory subunit that shields mitochondria from Ca(2+) overload. Before the identification of these core elements, the novel uncoupling proteins 2 and 3 (UCP2/3) have been shown to be fundamental for mitochondrial Ca(2+) uptake...
September 19, 2016: Nature Communications
https://www.readbyqxmd.com/read/27577262/proteome-wide-analysis-of-arginine-monomethylation-reveals-widespread-occurrence-in-human-cells
#13
Sara C Larsen, Kathrine B Sylvestersen, Andreas Mund, David Lyon, Meeli Mullari, Maria V Madsen, Jeremy A Daniel, Lars J Jensen, Michael L Nielsen
The posttranslational modification of proteins by arginine methylation is functionally important, yet the breadth of this modification is not well characterized. Using high-resolution mass spectrometry, we identified 8030 arginine methylation sites within 3300 human proteins in human embryonic kidney 293 cells, indicating that the occurrence of this modification is comparable to phosphorylation and ubiquitylation. A site-level conservation analysis revealed that arginine methylation sites are less evolutionarily conserved compared to arginines that were not identified as modified by methylation...
2016: Science Signaling
https://www.readbyqxmd.com/read/27571165/protein-arginine-methyltransferase-1-is-a-novel-regulator-of-mycn-in-neuroblastoma
#14
Allison Eberhardt, Jeanne N Hansen, Jan Koster, Louis T Lotta, Simeng Wang, Emmett Livingstone, Kun Qian, Linda J Valentijn, Yujun George Zheng, Nina F Schor, Xingguo Li
Amplification or overexpression of MYCN is associated with poor prognosis of human neuroblastoma. We have recently defined a MYCN-dependent transcriptional signature, including protein arginine methyltransferase 1 (PRMT1), which identifies a subgroup of patients with high-risk disease. Here we provide several lines of evidence demonstrating PRMT1 as a novel regulator of MYCN and implicating PRMT1 as a potential therapeutic target in neuroblastoma pathogenesis. First, we observed a strong correlation between MYCN and PRMT1 protein levels in primary neuroblastoma tumors...
September 27, 2016: Oncotarget
https://www.readbyqxmd.com/read/27545444/understanding-protein-arginine-methyltransferase-1-prmt1-product-specificity-from-molecular-dynamics
#15
Symon Gathiaka, Brittany Boykin, Tamar Cáceres, Joan M Hevel, Orlando Acevedo
Protein arginine methyltransferases (PRMTs) catalyze the post-translational methylation of specific arginyl groups within targeted proteins to regulate fundamental biological responses in eukaryotic cells. The major Type I PRMT enzyme, PRMT1, strictly generates monomethyl arginine (MMA) and asymmetric dimethylarginine (ADMA), but not symmetric dimethylarginine (SDMA). Multiple diseases can arise from the dysregulation of PRMT1, including heart disease and cancer, which underscores the need to elucidate the origin of product specificity...
October 15, 2016: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/27521225/lactate-induced-hif-1%C3%AE-prmt1-cross-talk-affects-mhc-i-expression-in-monocytes
#16
Piyushi Gupta, Ankita Singh, Pruthvi Gowda, Sadashib Ghosh, Arpita Chatterjee, Ellora Sen
Tumor infiltrating monocytes play a crucial role in tumor immune surveillance. As lactate is an important component of the tumor milieu, we investigated its role in the transcriptional regulation of MHC I which is crucial for mounting effective immune responses against tumors. Lactate elevated MHC class I expression in monocytes. Increase in HLAB expression was concomitant with increase in HIF-1α and decrease in PRMT1 levels. Interestingly, a reciprocal relationship was observed between PRMT1 and HIF-1α. While HIF-1α inhibition decreased lactate induced MHC I, both pharmacological inhibition and siRNA mediated knockdown of PRMT1 upregulated HLAB levels...
October 1, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27520967/arginine-methylation-enhances-the-rna-chaperone-activity-of-the-west-nile-virus-host-factor-auf1-p45
#17
Susann Friedrich, Tobias Schmidt, Angelika Schierhorn, Hauke Lilie, Grit Szczepankiewicz, Sandra Bergs, Uwe G Liebert, Ralph P Golbik, Sven-Erik Behrens
A prerequisite for the intracellular replication process of the Flavivirus West Nile virus (WNV) is the cyclization of the viral RNA genome, which enables the viral replicase to initiate RNA synthesis. Our earlier studies indicated that the p45 isoform of the cellular AU-rich element binding protein 1 (AUF1) has an RNA chaperone activity, which supports RNA cyclization and viral RNA synthesis by destabilizing a stem structure at the WNV RNA's 3'-end. Here we show that in mammalian cells, AUF1 p45 is consistently modified by arginine methylation of its C terminus...
October 2016: RNA
https://www.readbyqxmd.com/read/27466704/protein-arginine-methylation-facilitates-kcnq-channel-pip2-interaction-leading-to-seizure-suppression
#18
Hyun-Ji Kim, Myong-Ho Jeong, Kyung-Ran Kim, Chang-Yun Jung, Seul-Yi Lee, Hanna Kim, Jewoo Koh, Tuan Anh Vuong, Seungmoon Jung, Hyunwoo Yang, Su-Kyung Park, Dahee Choi, Sung Hun Kim, KyeongJin Kang, Jong-Woo Sohn, Joo Min Park, Daejong Jeon, Seung-Hoi Koo, Won-Kyung Ho, Jong-Sun Kang, Seong-Tae Kim, Hana Cho
KCNQ channels are critical determinants of neuronal excitability, thus emerging as a novel target of anti-epileptic drugs. To date, the mechanisms of KCNQ channel modulation have been mostly characterized to be inhibitory via Gq-coupled receptors, Ca(2+)/CaM, and protein kinase C. Here we demonstrate that methylation of KCNQ by protein arginine methyltransferase 1 (Prmt1) positively regulates KCNQ channel activity, thereby preventing neuronal hyperexcitability. Prmt1+/- mice exhibit epileptic seizures. Methylation of KCNQ2 channels at 4 arginine residues by Prmt1 enhances PIP2 binding, and Prmt1 depletion lowers PIP2 affinity of KCNQ2 channels and thereby the channel activities...
July 28, 2016: ELife
https://www.readbyqxmd.com/read/27338245/using-oriented-peptide-array-libraries-to-evaluate-methylarginine-specific-antibodies-and-arginine-methyltransferase-substrate-motifs
#19
Sitaram Gayatri, Martis W Cowles, Vidyasiri Vemulapalli, Donghang Cheng, Zu-Wen Sun, Mark T Bedford
Signal transduction in response to stimuli relies on the generation of cascades of posttranslational modifications that promote protein-protein interactions and facilitate the assembly of distinct signaling complexes. Arginine methylation is one such modification, which is catalyzed by a family of nine protein arginine methyltransferases, or PRMTs. Elucidating the substrate specificity of each PRMT will promote a better understanding of which signaling networks these enzymes contribute to. Although many PRMT substrates have been identified, and their methylation sites mapped, the optimal target motif for each of the nine PRMTs has not been systematically addressed...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27183873/methylation-of-arginine-by-prmt1-regulates-nrf2-transcriptional-activity-during-the-antioxidative-response
#20
Xin Liu, Hongyuan Li, Lingxia Liu, Yang Lu, Yanyan Gao, Pengyu Geng, Xiaoxue Li, Baiqu Huang, Yu Zhang, Jun Lu
The cap 'n' collar (CNC) family of transcription factors play important roles in resistance of oxidative and electrophilic stresses. Among the CNC family members, NF-E2-related factor 2 (Nrf2) is critical for regulating the antioxidant and phase II enzymes through antioxidant response element (ARE)-mediated transactivation. The activity of Nrf2 is controlled by a variety of post-translational modifications, including phosphorylation, ubiquitination, acetylation and sumoylation. Here we demonstrate that the arginine methyltransferase-1 (PRMT1) methylates Nrf2 protein at a single residue of arginine 437, both in vitro and in vivo...
August 2016: Biochimica et Biophysica Acta
keyword
keyword
115446
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"