Xiufang Liu, Ning Rong, Zhenhua Tian, Joseph Rich, Lili Niu, Pengqi Li, Laixin Huang, Yankai Dong, Wei Zhou, Pengfei Zhang, Yizhao Chen, Congzhi Wang, Long Meng, Tony Jun Huang, Hairong Zheng
Transfected stem cells and T cells are promising in personalized cell therapy and immunotherapy against various diseases. However, existing transfection techniques face a fundamental trade-off between transfection efficiency and cell viability; achieving both simultaneously remains a substantial challenge. This study presents an acoustothermal transfection method that leverages acoustic and thermal effects on cells to enhance the permeability of both the cell membrane and nuclear envelope to achieve safe, efficient, and high-throughput transfection of primary T cells and stem cells...
April 19, 2024: Science Advances