Miaomiao Li, Huaizhi Zhang, Huixin Xiao, Keyu Zhu, Wenqi Shi, Dong Zhang, Yong Wang, Lijun Yang, Qiuhong Wu, Jingzhong Xie, Yongxing Chen, Dan Qiu, Guanghao Guo, Ping Lu, Beibei Li, Lei Dong, Wenling Li, Xuejia Cui, Lingchuan Li, Xiubin Tian, Chengguo Yuan, Yiwen Li, Dazhao Yu, Eviatar Nevo, Tzion Fahima, Hongjie Li, Lingli Dong, Yusheng Zhao, Zhiyong Liu
Crop wild relatives offer natural variations of disease resistance for crop improvement. Here, we report the isolation of broad-spectrum powdery mildew resistance gene Pm36, originated from wild emmer wheat, that encodes a tandem kinase with a transmembrane domain (WTK7-TM) through the combination of map-based cloning, PacBio SMRT long-read genome sequencing, mutagenesis, and transformation. Mutagenesis assay reveals that the two kinase domains and the transmembrane domain of WTK7-TM are critical for the powdery mildew resistance function...
April 10, 2024: Nature Communications