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Hélène Guyon, François Mavré, Marjorie Catala, Serge Turcaud, Franck Brachet, Benoît Limoges, Carine Tisné, Laurent Micouin
In this work, we report an affordable, sensitive, fast and user-friendly electroanalytical method for monitoring the binding between unlabeled RNA and small compounds in microliter-size droplets using a redox-probe and disposable miniaturized screen-printed electrochemical cells.
January 5, 2017: Chemical Communications: Chem Comm
Aggeliki Kolialexi, Danai Mavreli, Nikolas Papantoniou
No abstract text is available yet for this article.
February 2017: Expert Review of Proteomics
Lylian Challier, Rebeca Miranda-Castro, Bertrand Barbe, Claire Fave, Benoît Limoges, Eric Peyrin, Corinne Ravelet, Emmanuelle Fiore, Pierre Labbé, Liliane Coche-Guérente, Eric Ennifar, Guillaume Bec, Philippe Dumas, François Mavré, Vincent Noël
Nucleic acid aptamers are involved in a broad field of applications ranging from therapeutics to analytics. Deciphering the binding mechanisms between aptamers and small ligands is therefore crucial to improve and optimize existing applications and to develop new ones. Particularly interesting is the enantiospecific binding mechanism involving small molecules with nonprestructured aptamers. One archetypal example is the chiral binding between l-tyrosinamide and its 49-mer aptamer for which neither structural nor mechanistic information is available...
December 6, 2016: Analytical Chemistry
R Oliveira, S Groni, C Fave, M Branca, F Mavré, D Lorcy, M Fourmigué, B Schöllhorn
The halogen bond donor properties of iodo-tetrathiafulvalene (I-TTF) can be electrochemically switched and controlled via reversible oxidation in the solution phase. Interestingly the activation of only one single halogen bond yielded already a strong and selective interaction, quantified by cyclic voltammetry. The standard potentials of the redox couples I-TTF(0/1+) and I-TTF(1+/2+) were observed to shift upon the addition of halides. These anions selectively stabilize the cationic I-TTF species through halogen bonding in polar liquid electrolytes...
June 21, 2016: Physical Chemistry Chemical Physics: PCCP
Alexandra Lycoudi, Danai Mavreli, Ariadni Mavrou, Nikolas Papantoniou, Aggeliki Kolialexi
MicroRNAs (miRNAs) constitute a highly conserved class of small non-coding RNAs, involved in post-transcriptional regulation processes by modifying the expression of specific mRNAs. During placental development, cell differentiation, adhesion, migration, apoptosis and angiogenesis are regulated by specific miRNAs and aberrant expression has been associated with the pathogenesis of pregnancy-related complications. Recent studies focusing on placental and maternal peripheral blood miRNA profiling showed different expression between normal and complicated pregnancies, providing valuable information about the pathophysiological role of miRNAs and identifying potential biomarkers for monitoring pregnancy complications...
2015: Expert Review of Molecular Diagnostics
Aggeliki Kolialexi, Danai Mavreli, Georgia Tounta, Ariadni Mavrou, Nikolas Papantoniou
Preeclampsia (PE) is a multisystem disorder of pregnancy that develops after 20 wk of gestation in previously normotensive women and complicates 5-8% of pregnancies. This rapidly progressive syndrome is usually diagnosed when the mother develops hypertension and proteinuria. The only effective treatment is delivery of the baby although early low-dose aspirin has been shown to significantly reduce the risk for PE. Recent advances in proteomic methods of protein separation, identification, and quantitation may allow for the identification of proteins and peptides that could facilitate early detection of disease, improve assessment of prognosis, and allow closer monitoring of women at risk for PE...
June 2015: Proteomics. Clinical Applications
Aggeliki Kolialexi, Dimitrios Gourgiotis, George Daskalakis, Antonis Marmarinos, Alexandra Lykoudi, Danai Mavreli, Ariadni Mavrou, Nikolas Papantoniou
AIM: To examine the potential value of previously identified biomarkers using proteomics in early screening for preeclampsia (PE). METHODS: 24 blood samples from women who subsequently developed PE and 48 from uncomplicated pregnancies were obtained at 11-13 weeks and analysed after delivery. Cystatin-C, sVCAM-1, and Pappalysin-1 were quantified by ELISA. Maternal characteristics and medical history were recorded. RESULTS: Median values of Cystatin-C, sVCAM-1, and Pappalysin-1 in the PE group as compared to controls were 909...
2015: Disease Markers
Ju Y Lim, Salil V Deo, Salah E Altarabsheh, Sung H Jung, Patricia J Erwin, Alan H Markowitz, Soon J Park
BACKGROUND: Minimally invasive aortic valve replacement (mAVR) is increasingly preferred over conventional AVR (cAVR). However, data comparing these procedures present conflicting results. Hence, we conducted a systematic review and meta-analysis comparing clinical results in these cohorts. METHOD: Only randomized controlled trials (RCT) and propensity-matched observational studies (POS) (1998-2013) comparing clinical outcome of patients subjected to mAVR or cAVR were pooled...
February 2015: Journal of Cardiac Surgery
B M Swinkels, B A de Mol, J C Kelder, F E Vermeulen, J M Ten Berg
BACKGROUND: Although younger patients are supposed to be less susceptible to bleeding complications of mechanical aortic valve replacement (mAVR) than older patients, there is a relative paucity of data on this subject. Therefore, it remains uncertain whether younger patients are really at a lower risk of these complications than older patients. METHODS: Incidence rates of bleeding events during 15 years of follow-up after mAVR were compared between 163 patients under 60 (group I), 122 patients between 60 and 65 (group II), and 145 patients over 65 (group III) years of age at operation...
February 2015: Netherlands Heart Journal
Reginald C W Bell, William E Barnes, Bart J Carter, Robert W Sewell, Peter G Mavrelis, Glenn M Ihde, Kevin M Hoddinott, Mark A Fox, Katherine D Freeman, Tanja Gunsberger, Mark G Hausmann, David Dargis, Brian DaCosta Gill, Erik Wilson, Karim S Trad
The aim of this study was to assess prospectively 2-year outcomes of transoral incisionless fundoplication (TIF) in a multicenter setting. A 14-center U.S. registry was designed to evaluate the effects of the TIF 2.0 procedure on chronic gastroesophageal reflux disease (GERD) in over 100 patients. Primary outcome was symptom assessment. Secondary outcomes were proton pump inhibitor (PPI) use, degree of esophagitis, safety, and changes in esophageal acid exposure. One hundred twenty-seven patients underwent TIF between January 2010 and April 2011, 19 (15%) of whom were lost to follow-up...
November 2014: American Surgeon
Karim Sami Trad, Gilbert Simoni, William Edris Barnes, Ahmad Bassel Shughoury, Mamoon Raza, Jeffrey Alan Heise, Daniel Gilles Turgeon, Mark Alan Fox, Peter George Mavrelis
BACKGROUND: The aim of this randomized, crossover study was to determine if transoral fundoplication (TF) could further improve clinical outcomes in partial responders to high-dose (HD) proton-pump inhibitor (PPI) therapy and to evaluate durability of TF. METHODS: In seven United States centers, patients with hiatal hernia ≤ 2 cm and abnormal esophageal acid exposure (EAE) were randomized to TF (n = 40) or HD PPIs (n = 23) group. At 6-month follow-up, PPI patients underwent crossover...
October 6, 2014: BMC Gastroenterology
Reginald C W Bell, Mark A Fox, William E Barnes, Peter G Mavrelis, Robert W Sewell, Bart J Carter, Glenn M Ihde, Karim S Trad, David Dargis, Kevin M Hoddinott, Katherine D Freeman, Tanja Gunsberger, Mark G Hausmann, Brian DaCosta Gill, Erik Wilson
BACKGROUND: Preoperative factors predicting symptomatic improvement after transoral fundoplication (TF) in chronic gastroesophageal reflux disease (GERD) patients with persistent symptoms on proton-pump inhibitors (PPIs) therapy have not been elucidated fully. METHODS: Univariate and multivariate logistic regression analyses were performed on data from 158 consecutive patients who underwent TF with the EsophyX device between January 2010 and June 2012 in 14 community centers...
October 2014: Surgical Endoscopy
Karim S Trad, William E Barnes, Gilbert Simoni, Ahmad B Shughoury, Peter G Mavrelis, Mamoon Raza, Jeffrey A Heise, Daniel G Turgeon, Mark A Fox
BACKGROUND: Incomplete control of troublesome regurgitation and extraesophageal manifestations of chronic gastroesophageal reflux disease (GERD) is a known limitation of proton pump inhibitor (PPI) therapy. This multicenter randomized study compared the efficacy of transoral incisionless fundoplication (TIF) against PPIs in controlling these symptoms in patients with small hiatal hernias. METHODS: Between June and August 2012, 63 patients were randomized at 7 US community hospitals...
February 2015: Surgical Innovation
Joseph S Coselli, Irina V Volguina, Scott A LeMaire, Thoralf M Sundt, Heidi M Connolly, Elizabeth H Stephens, Hartzell V Schaff, Dianna M Milewicz, Luca A Vricella, Harry C Dietz, Charles G Minard, D Craig Miller
OBJECTIVE: To compare the 1-year results after aortic valve-sparing (AVS) or valve-replacing (AVR) aortic root replacement from a prospective, international registry of 316 patients with Marfan syndrome (MFS). METHODS: Patients underwent AVS (n = 239, 76%) or AVR (n = 77, 24%) aortic root replacement at 19 participating centers from 2005 to 2010. One-year follow-up data were complete for 312 patients (99%), with imaging findings available for 293 (94%). The time-to-events were compared between groups using Kaplan-Meier curves and Cox proportional hazards models...
June 2014: Journal of Thoracic and Cardiovascular Surgery
Julie Moreau, Lylian Challier, Noémie Lalaoui, François Mavré, Vincent Noël, Benoît Limoges, Bernd Schöllhorn, Claire Fave
A series of redox-labeled L-tyrosinamide (L-Tym) derivatives was prepared and the nature of the functional group and the chain length of the spacer were systematically varied in a step-by-step affinity optimization process of the tracer for the L-Tym aptamer. The choice of the labeling position on L-Tym proved to be crucial for the molecular recognition event, which could be monitored by cyclic voltammetry and is based on the different diffusion rates of free and bound targets in solution. From this screening approach an efficient electroactive tracer emerged...
March 3, 2014: Chemistry: a European Journal
Erik B Wilson, William E Barnes, Peter G Mavrelis, Bart J Carter, Reginald C W Bell, Robert W Sewell, Glenn M Ihde, David Dargis, Kevin M Hoddinott, Ahmad B Shughoury, Brian D Gill, Mark A Fox, Daniel G Turgeon, Katherine D Freeman, Tanja Gunsberger, Mark G Hausmann, Karl A Leblanc, Emir Deljkich, Karim S Trad
PURPOSE: This study aimed to assess the impact of transoral incisionless fundoplication (TIF) on patients with chronic gastroesophageal reflux disease (GERD) at 12-month follow-up. METHODS: Clinical outcomes of 100 consecutive patients with chronic GERD who underwent TIF between January 2010 and February 2011 were analyzed. RESULTS: There were no major complications reported. Esophageal acid exposure was normalized in 14/27 (52%) of patients who underwent 12-month pH testing...
February 2014: Surgical Laparoscopy, Endoscopy & Percutaneous Techniques
Ling Zhang, Rebeca Miranda-Castro, Claire Stines-Chaumeil, Nicolas Mano, Guobao Xu, François Mavré, Benoît Limoges
A highly sensitive electroanalytical method for determination of PQQ in solution down to subpicomolar concentrations is proposed. It is based on the heterogeneous reconstitution of the PQQ-dependent glucose dehydrogenase (PQQ-GDH) through the specific binding of its pyrroloquinoline quinone (PQQ) cofactor to the apoenzyme anchored on an electrode surface. It is shown from kinetics analysis of both the enzyme catalytic responses and enzyme surface-reconstitution process (achieved by cyclic voltammetry under redox-mediated catalysis) that the selected immobilization strategy (i...
February 18, 2014: Analytical Chemistry
Lylian Challier, Rebeca Miranda-Castro, Damien Marchal, Vincent Noël, François Mavré, Benoît Limoges
Here, we demonstrate a new generic, affordable, simple, versatile, sensitive, and easy-to-implement electrochemical kinetic method for monitoring, in real time, the progress of a chemical or biological reaction in a microdrop of a few tens of microliters, with a kinetic time resolution of ca. 1 s. The methodology is based on a fast injection and mixing of a reactant solution (1-10 μL) in a reaction droplet (15-50 μL) rapidly rotated over the surface of a nonmoving working electrode and on the recording of the ensuing transient faradaic current associated with the transformation of one of the components...
September 25, 2013: Journal of the American Chemical Society
Reginald C W Bell, Peter G Mavrelis, William E Barnes, David Dargis, Bart J Carter, Kevin M Hoddinott, Robert W Sewell, Karim S Trad, Brian DaCosta Gill, Glenn M Ihde
BACKGROUND: This study was undertaken to validate previously reported safety and symptomatic outcomes of transoral incisionless fundoplication (TIF), evaluate the relative benefit of TIF within different gastroesophageal reflux disease (GERD) subgroups, and to determine predictors of success in community settings. STUDY DESIGN: Between January 2010 and February 2011, 100 consecutive patients who underwent TIF procedures at 10 centers were enrolled in this prospective, open-label, multicenter, single-arm study...
December 2012: Journal of the American College of Surgeons
Rebeca Miranda-Castro, Damien Marchal, Benoît Limoges, François Mavré
A simple and fast electrochemical nucleic acid assay based on a target recycling strategy and the release of a double-stranded DNA intercalating redox probe upon digestion of a specific duplex by exonuclease III is demonstrated.
September 11, 2012: Chemical Communications: Chem Comm
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