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https://www.readbyqxmd.com/read/28545271/-effect-of-microrna-106b-on-the-invasion-and-proliferation-of-trophoblasts-through-targeting-mmp-2
#1
J Li, J M Wang, Y H Liu, Z Zhang, N Han, J Y Wang, S H Xue, P Wang
Objective: To investigate the expression of microRNA-106b (miR-106b) in the placentas of patients with pre-eclampsia and its relationship with matrix metallopeptidase (MMP) -2, and its effect on the invasion and proliferation of trophoblasts. Methods: (1) Placental tissues were collected from patients with mild pre-eclampsia (mPE, n=30), severe pre-eclampsia (sPE, n=30) and normal pregnant women (n=40). Human choriocarcinoma cell lines JAR and JEG3 were assigned to the miR-106b mimics group, the mimics negative control group, the miR-106b inhibitor group and the inhibitor negative control group, respectively...
May 25, 2017: Zhonghua Fu Chan Ke za Zhi
https://www.readbyqxmd.com/read/28544929/rem-sleep-and-memory
#2
REVIEW
Richard Boyce, Sylvain Williams, Antoine Adamantidis
Memory consolidation, a process which stabilizes recently acquired information into long-term storage, is thought to be optimized during sleep. Although recent evidence indicates that non-rapid-eye movement sleep (NREMs) is directly involved in memory consolidation, the role of rapid-eye movement sleep (REMs) in this process has remained controversial due to the extreme difficulty in experimentally isolating neural activity during REMs. Using a combination of electrophysiological recording and optogenetic techniques, recent work demonstrated for the first time that neural activity occurring specifically during REMs is required for spatial and contextual memory consolidation...
May 22, 2017: Current Opinion in Neurobiology
https://www.readbyqxmd.com/read/28544786/mir-122-modification-enhances-the-therapeutic-efficacy-of-adipose-tissue-derived-mesenchymal-stem-cells-against-liver-fibrosis
#3
Guohua Lou, Ying Yang, Feifei Liu, Bingjue Ye, Zhi Chen, Min Zheng, Yanning Liu
Mesenchymal stem cell (MSC) transplantation alone may be insufficient for treatment of liver fibrosis because of complicated histopathological changes in the liver. Given that miR-122 plays an essential role in liver fibrosis by negatively regulating the proliferation and transactivation of hepatic stellate cells (HSCs), this study investigated whether miR-122 modification can improve the therapeutic efficacy of adipose tissue-derived MSCs in treating liver fibrosis. MiR-122-modified AMSCs (AMSC-122) were constructed through lentivirus-mediated transfer of pre-miR-122...
May 24, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28544111/kallistatin-reduces-vascular-senescence-and-aging-by-regulating-microrna-34a-sirt1-pathway
#4
Youming Guo, Pengfei Li, Lin Gao, Jingmei Zhang, Zhirong Yang, Grant Bledsoe, Eugene Chang, Lee Chao, Julie Chao
Kallistatin, an endogenous protein, protects against vascular injury by inhibiting oxidative stress and inflammation in hypertensive rats and enhancing the mobility and function of endothelial progenitor cells (EPCs). We aimed to determine the role and mechanism of kallistatin in vascular senescence and aging using cultured EPCs, streptozotocin (STZ)-induced diabetic mice, and Caenorhabditis elegans (C. elegans). Human kallistatin significantly decreased TNF-α-induced cellular senescence in EPCs, as indicated by reduced senescence-associated β-galactosidase activity and plasminogen activator inhibitor-1 expression, and elevated telomerase activity...
May 24, 2017: Aging Cell
https://www.readbyqxmd.com/read/28543398/interdigital-tissue-remodelling-in-the-embryonic-limb-involves-dynamic-regulation-of-the-mirna-profiles
#5
Beatriz Garcia-Riart, Carlos I Lorda-Diez, Jessica C Marin-Llera, Juan A Garcia-Porrero, Juan M Hurle, Juan A Montero
Next-generation sequencing in combination with quantitative polymerase chain reaction analysis revealed a dynamic miRNA signature in the interdigital mesoderm of the chick embryonic hinlimb in the course of interdigit remodelling. During this period, 612 previously known chicken miRNAs (gga-miRNAs) and 401 non-identified sequences were expressed in the interdigital mesoderm. Thirty-six microRNAs, represented by more than 750 reads per million, displayed differential expression between stages HH29 (6 id) and HH32 (7...
May 24, 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/28542740/plasma-micrornas-as-biomarkers-of-pancreatic-cancer-risk-in-a-prospective-cohort-study
#6
Eric J Duell, Leila Lujan-Barroso, Núria Sala, Samantha Deitz McElyea, Kim Overvad, Anne Tjonneland, Anja Olsen, Elisabete Weiderpass, Lill-Tove Busund, Line Moi, David Muller, Paolo Vineis, Dagfinn Aune, Giuseppe Matullo, Alessio Naccarati, Salvatore Panico, Giovanna Tagliabue, Rosario Tumino, Domenico Palli, Rudolf Kaaks, Verena A Katzke, Heiner Boeing, H B As Bueno-de-Mesquita, Petra H Peeters, Antonia Trichopoulou, Pagona Lagiou, Anastasia Kotanidou, Ruth C Travis, Nick Wareham, Kay-Tee Khaw, Jose Ramon Quiros, Miguel Rodríguez-Barranco, Miren Dorronsoro, María-Dolores Chirlaque, Eva Ardanaz, Gianluca Severi, Marie-Christine Boutron-Ruault, Vinciane Rebours, Paul Brennan, Marc Gunter, Ghislaine Scelo, Greg Cote, Stuart Sherman, Murray Korc
Non-invasive biomarkers for early pancreatic ductal adenocarcinoma (PDAC) diagnosis and disease risk stratification are greatly needed. We conducted a nested case-control study within the Prospective Investigation into Cancer and Nutrition (EPIC) cohort to evaluate pre-diagnostic microRNAs (miRs) as biomarkers of subsequent PDAC risk. A panel of eight miRs (miR-10a, -10b, -21-3p, -21-5p, -30c, -106b, -155, and -212) based on previous evidence from our group was evaluated in 225 microscopically confirmed PDAC cases and 225 controls matched on center, sex, fasting status, and age/date/time of blood collection...
May 19, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28542195/differential-excitatory-control-of-2-parallel-basket-cell-networks-in-amygdala-microcircuits
#7
Tibor Andrási, Judit M Veres, Laura Rovira-Esteban, Richárd Kozma, Attila Vikór, Erzsébet Gregori, Norbert Hájos
Information processing in neural networks depends on the connectivity among excitatory and inhibitory neurons. The presence of parallel, distinctly controlled local circuits within a cortical network may ensure an effective and dynamic regulation of microcircuit function. By applying a combination of optogenetics, electrophysiological recordings, and high resolution microscopic techniques, we uncovered the organizing principles of synaptic communication between principal neurons and basket cells in the basal nucleus of the amygdala...
May 2017: PLoS Biology
https://www.readbyqxmd.com/read/28542140/mir-21-ablation-and-obeticholic-acid-ameliorate-nonalcoholic-steatohepatitis-in-mice
#8
Pedro M Rodrigues, Marta B Afonso, André L Simão, Catarina C Carvalho, Alexandre Trindade, António Duarte, Pedro M Borralho, Mariana V Machado, Helena Cortez-Pinto, Cecília Mp Rodrigues, Rui E Castro
This corrects the article DOI: 10.1038/cddis.2017.172.
May 25, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28541889/distinct-responses-of-purkinje-neurons-and-roles-of-simple-spikes-during-associative-motor-learning-in-larval-zebrafish
#9
Thomas C Harmon, Uri Magaram, David L McLean, Indira M Raman
To study cerebellar activity during learning, we made whole-cell recordings from larval zebrafish Purkinje cells while monitoring fictive swimming during associative conditioning. Fish learned to swim in response to visual stimulation preceding tactile stimulation of the tail. Learning was abolished by cerebellar ablation. All Purkinje cells showed task-related activity. Based on how many complex spikes emerged during learned swimming, they were classified as multiple, single, or zero complex spike (MCS, SCS, ZCS) cells...
May 25, 2017: ELife
https://www.readbyqxmd.com/read/28541153/evaluation-of-the-effects-of-a-4-7-mg-deslorelin-acetate-implant-on-egg-laying-in-cockatiels-nymphicus-hollandicus
#10
Noémie M Summa, David Sanchez-Migallon Guzman, Emma L Wils-Plotz, Nerisa E Riedl, Philip H Kass, Michelle G Hawkins
OBJECTIVE To evaluate effects of administration of a 4.7-mg deslorelin acetate implant on egg laying in healthy cockatiels (Nymphicus hollandicus). ANIMALS 52 cockatiels. PROCEDURES 26 breeding pairs (a female and its respective male in each pair) were selected on the basis of their history of egg laying. Female birds were sedated and received a 4.7-mg deslorelin acetate implant (n = 13) or placebo implant (13) in the subcutaneous tissues between the scapulae. Male and female birds of each breeding pair were placed in separate but adjacent cages...
June 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28540063/microrna-122-ameliorates-corneal-allograft-rejection-through-the-downregulation-of-its-target-cpeb1
#11
Ting Wang, Fengjie Li, Wenwen Geng, Qingguo Ruan, Weiyun Shi
Transplant rejection is a major cause of corneal transplantation failure. MicroRNAs (miRNAs) are a family of small RNAs that regulates gene expression in a sequence-specific manner. miRNAs have recently been shown to have important roles in human organ transplantation, but reports of miRNAs directly associated with corneal transplantation rejection remain limited. To investigate the role of miRNAs during corneal allograft rejection, we established a mouse penetrating keratoplasty model and used microarrays to screen for differentially expressed miRNAs...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28539838/andrographolide-inhibits-angiogenesis-by-inhibiting-the-mir-21-5p-timp3-signaling-pathway
#12
Jianwei Dai, Yuyin Lin, Youfa Duan, Zixuan Li, Dalei Zhou, Wensheng Chen, Lijing Wang, Qian-Qian Zhang
Angiogenesis provides nutrients and oxygen to promote tumor growth and affords a channel that facilitates tumor cell entry into the circulation. Andrographolide (Andro) possess anti-tumor activity; however, its direct effect on angiogenesis still needs to be clarified. In this study, our experiments revealed that Andro significantly inhibited vascular growth in chick embryo chorioallantoic membrane (CAM) and yolk sac membrane (YSM) models. Meanwhile, tumor angiogenesis was also suppressed by Andro. Additionally, we found that cell proliferation, migration and tube formation of vascular endothelial cells was inhibited by Andro treatment in vitro...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28539613/optogenetic-excitation-of-cholinergic-inputs-to-hippocampus-primes-future-contextual-fear-associations
#13
Sarah Hersman, Jesse Cushman, Noah Lemelson, Kate Wassum, Shahrdad Lotfipour, Michael S Fanselow
Learning about context is essential for appropriate behavioral strategies, but important contingencies may not arise during initial learning. A variant of contextual fear conditioning, context pre-exposure facilitation, allows us to directly test the relationship between novelty-induced acetylcholine release and later contextual associability. We demonstrate that optogenetically-enhanced acetylcholine during initial contextual exploration leads to stronger fear after subsequent pairing with shock, suggesting that novelty-induced acetylcholine release primes future contextual associations...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28539478/a-novel-epigenetic-aml1-eto-thap10-mir-383-mini-circuitry-contributes-to-t-8-21-leukaemogenesis
#14
Yonghui Li, Qiaoyang Ning, Jinlong Shi, Yang Chen, Mengmeng Jiang, Li Gao, Wenrong Huang, Yu Jing, Sai Huang, Anqi Liu, Zhirui Hu, Daihong Liu, Lili Wang, Clara Nervi, Yun Dai, Michael Q Zhang, Li Yu
DNA methylation patterns are frequently deregulated in t(8;21) acute myeloid leukaemia (AML), but little is known of the mechanisms by which specific gene sets become aberrantly methylated. Here, we found that the promoter DNA methylation signature of t(8;21)(+) AML blasts differs from that of t(8;21)(-) AMLs. This study demonstrated that a novel hypermethylated zinc finger-containing protein, THAP10, is a target gene and can be epigenetically suppressed by AML1-ETO at the transcriptional level in t(8;21) AML...
May 24, 2017: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/28539416/the-extinction-of-cocaine-seeking-requires-a-window-of-infralimbic-pyramidal-neuron-activity-after-unreinforced-lever-presses
#15
Andrea L Gutman, Kelle E Nett, Caitlin V Cosme, Wensday R Worth, Subhash C Gupta, John A Wemmie, Ryan T LaLumiere
The infralimbic cortex (IL) mediates the extinction learning and active suppression of cocaine-seeking behavior. However, the precise temporal relationship among IL activity, lever pressing, and extinction learning is unclear. To address this issue, we employed activity-guided optogenetics in male Sprague-Dawley rats to optically silence IL pyramidal neurons for 20 s immediately following unreinforced lever presses during early extinction training after cocaine self-administration. Optical inhibition of the IL increased active lever pressing during shortened extinction sessions but did not alter the retention of the extinction learning as assessed in ensuing extinction sessions with no optical inhibition...
May 24, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28539288/-predictive-value-of-mirna-29a-and-mirna-10a-5p-for-28-day-mortality-in-patients-with-sepsis-induced-acute-kidney-injury
#16
Rui Huo, Min Dai, Yi Fan, Jing-Zheng Zhou, Li Li, Jian Zu
OBJECTIVE: To investigate the value of miR-29a and miR-10a-5p in predicting 28-day mortality in patients with sepsis-induced acute kidney injury. METHODS: Seventy-four patients with sepsis-induced acute kidney injury (AKI) and 41 patients with sepsis but without AKI (control) were examined for serum levels of miR-29a and miR-10a-5p using RT-PCR. The patients were followed up for 28 days to record their survival. Pearson correlation analysis was used to test the correlations of miR-29a and miR-10a-5p with serum creatinine (Scr), cystatin C (Cys-C), and KIM-1 in patients with AKI...
May 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28538173/cortico-accumbens-regulation-of-approach-avoidance-behavior-is-modified-by-experience-and-chronic-pain
#17
Neil Schwartz, Catriona Miller, Howard L Fields
Although optimizing decisions between drives to avoid pain and to obtain reward are critical for survival, understanding the neuronal circuit activity that regulates choice during approach-avoidance conflicts is limited. Here, we recorded neuronal activity in the infralimbic (IL) cortex and nucleus accumbens (NAc) during an approach-avoidance task. In this task, disruption of approach by a pain-predictive cue (PPC-avoidance) is extinguished by experience and reinstated in a model of chronic pain. In the IL-NAc circuit, the activity of distinct subpopulations of neurons predicts the extent of PPC-avoidance observed...
May 23, 2017: Cell Reports
https://www.readbyqxmd.com/read/28537917/microrna-210-suppresses-glucocorticoid-receptor-expression-in-response-to-hypoxia-in-fetal-rat-cardiomyocytes
#18
Shannalee R Martinez, Qingyi Ma, Chiranjib Dasgupta, Xianmei Meng, Lubo Zhang
Hypoxia is a common intrauterine stressor, often resulting in intrauterine growth restriction and increased risk for cardiovascular disease later in life. The aim of this work was to test the hypothesis that microRNA-210 (miR-210) mediates the detrimental suppression of glucocorticoid receptor (GR) in response to hypoxia in fetal rat cardiomyocytes. Cardiomyocytes isolated from gestational day 21 Sprague Dawley fetal rats showed increased miR-210 levels and reduced GR abundance after exposure to ex vivo hypoxia (1% O2)...
May 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28536507/dopamine-modulates-delta-gamma-phase-amplitude-coupling-in-the-prefrontal-cortex-of-behaving-rats
#19
Victoria Andino-Pavlovsky, Annie C Souza, Robson Scheffer-Teixeira, Adriano B L Tort, Roberto Etchenique, Sidarta Ribeiro
Dopamine release and phase-amplitude cross-frequency coupling (CFC) have independently been implicated in prefrontal cortex (PFC) functioning. To causally investigate whether dopamine release affects phase-amplitude comodulation between different frequencies in local field potentials (LFP) recorded from the medial PFC (mPFC) of behaving rats, we used RuBiDopa, a light-sensitive caged compound that releases the neurotransmitter dopamine when irradiated with visible light. LFP power did not change in any frequency band after the application of light-uncaged dopamine, but significantly strengthened phase-amplitude comodulation between delta and gamma oscillations...
2017: Frontiers in Neural Circuits
https://www.readbyqxmd.com/read/28536505/new-tools-to-study-astrocyte-ca-2-signal-dynamics-in-brain-networks-in-vivo
#20
REVIEW
Gabriele Losi, Letizia Mariotti, Michele Sessolo, Giorgio Carmignoto
Sensory information processing is a fundamental operation in the brain that is based on dynamic interactions between different neuronal populations. Astrocytes, a type of glial cells, have been proposed to represent active elements of brain microcircuits that, through dynamic interactions with neurons, provide a modulatory control of neuronal network activity. Specifically, astrocytes in different brain regions have been described to respond to neuronal signals with intracellular Ca(2+) elevations that represent a key step in the functional recruitment of astrocytes to specific brain circuits...
2017: Frontiers in Cellular Neuroscience
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