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https://www.readbyqxmd.com/read/29168386/carbon-nanotube-doped-adhesive-films-for-detecting-crack-propagation-on-bonded-joints-a-deeper-understanding-of-anomalous-behaviors
#1
Xoan Xosé Fernández Sánchez-Romate, Javier Molinero, Alberto Jiménez-Suárez, María Sánchez, Alfredo Güemes, Alejandro Ureña
A novel nanoreinforced adhesive film has been developed in order to detect adhesive deformation and crack propagation along the bonding line by means of the electrical response of the material. Adhesive films were doped by spraying an aqueous dispersion of carbon nanotubes (CNTs) over the surface. In order to determine the sensitivity of bonded joints, Single Lap Shear (SLS) and Mode-I fracture energy tests have been carried out while their electrical response has been measured. It has been found that CNT-doped adhesive films are able to detect adhesive deformation and final failure for SLS specimens and crack initiation and propagation along the bonding line for Mode-I specimens with a high sensitivity...
November 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29168105/evaluation-of-four-commercial-natural-products-for-repellency-and-toxicity-against-the-lone-star-tick-amblyomma-americanum-acari-ixodidae
#2
Erika T Machtinger, Andrew Y Li
Lone star ticks are aggressive ectoparasites of domestic and wild animals, as well as humans. These ticks can transmit many pathogens that cause disease including Erhlichia and tularemia. Common compounds used for personal protection and area sprays are N-diethyl-3-methyl benzamide (DEET) and permethrin, but public concern over personal and environmental safety require the development of new, safer products. In the current study, four commercially available products (Wondercide, Essentria IC(3), Vet's Best, and Mosquito Barrier) were tested for both repellent and toxic effects against lone star tick nymphs and adults...
November 22, 2017: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/29167574/structural-basis-of-cholesterol-binding-by-a-novel-clade-of-dendritic-cell-modulators-from-ticks
#3
Pietro Roversi, Steven Johnson, Stephen G Preston, Miles A Nunn, Guido C Paesen, Jonathan M Austyn, Patricia A Nuttall, Susan M Lea
Two crystal structures of Japanin, an 18 kDa immune-modulatory lipocalin from the Brown Ear Tick (Rhipicephalus appendiculatus), have been determined at 2.2 and 2.4 Å resolution. In both crystal forms the protein is in complex with cholesterol, which sits in a closed pocket at the centre of the lipocalin barrel. Both crystal forms are dimers, which are also observed in solution. Molecular modelling suggests that previously-described members of a tick protein family bearing high sequence homology to Japanin are also likely to bind cholesterol or cholesterol derivatives...
November 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29166863/characterization-of-the-inner-membrane-protein-bb0173-from-borrelia-burgdorferi
#4
Christina M Brock, Manuel Bañó-Polo, Maria J Garcia-Murria, Ismael Mingarro, Maria Esteve-Gasent
BACKGROUND: The bacterial spirochete Borrelia burgdorferi is the causative agent of the most commonly reported arthropod-borne illness in the United States, Lyme disease. A family of proteins containing von Willebrand Factor A (VWFA) domains adjacent to a MoxR AAA+ ATPase have been found to be highly conserved in the genus Borrelia. Previously, a VWFA domain containing protein of B. burgdorferi, BB0172, was determined to be an outer membrane protein capable of binding integrin α3β1...
November 22, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/29165231/sequence-optimized-real-time-reverse-transcription-polymerase-chain-reaction-assay-for-detection-of-crimean-congo-hemorrhagic-fever-virus
#5
Jeffrey W Koehler, Korey L Delp, Adrienne T Hall, Scott P Olschner, Brian J Kearney, Aura R Garrison, Louis A Altamura, Cynthia A Rossi, Timothy D Minogue
Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus of the genus Nairovirus within the family Bunyaviridae. Infection can result in general myalgia, fever, and headache with some patients developing hemorrhagic fever with mortality rates ranging from 5% to 30%. CCHFV has a wide geographic range that includes Africa, Asia, the Middle East, and Europe with nucleotide sequence variation approaching 20% across the three negative-sense RNA genome segments. While phylogenetic clustering generally aligns with geographic origin of individual strains, distribution can be wide due to tick/CCHFV dispersion via migrating birds...
November 20, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29164729/identification-and-characterization-of-a-histamine-binding-lipocalin-like-molecule-from-the-relapsing-fever-tick-ornithodoros-turicata
#6
G Neelakanta, H Sultana, D E Sonenshine, J F Andersen
Lipocalins are low molecular weight membrane transporters that are abundantly expressed in the salivary glands and other tissues of ticks. In this study, we identified a lipocalin-like molecule, designated as otlip, from the soft ticks Ornithodoros turicata, the vector for the relapsing fever causing spirochete Borrelia turicatae. We noted that the expression of otlip was developmentally regulated, with adult ticks expressing significantly higher levels in comparison to the larvae or nymphal ticks. Expression of otlip was evident in both fed and unfed O...
November 22, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29164067/tick-borne-transmission-of-murine-gammaherpesvirus-68
#7
Valeria Hajnická, Marcela Kúdelová, Iveta Štibrániová, Mirko Slovák, Pavlína Bartíková, Zuzana Halásová, Peter Pančík, Petra Belvončíková, Michaela Vrbová, Viera Holíková, Rosemary S Hails, Patricia A Nuttall
Herpesviruses are a large group of DNA viruses infecting mainly vertebrates. Murine gammaherpesvirus 68 (MHV68) is often used as a model in studies of the pathogenesis of clinically important human gammaherpesviruses such as Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus. This rodent virus appears to be geographically widespread; however, its natural transmission cycle is unknown. Following detection of MHV68 in field-collected ticks, including isolation of the virus from tick salivary glands and ovaries, we investigated whether MHV68 is a tick-borne virus...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29162709/ehrlichia-chaffeensis-and-its-invasin-etpe-block-reactive-oxygen-species-generation-by-macrophages-in-a-dnase-x-dependent-manner
#8
Omid Teymournejad, Mingqun Lin, Yasuko Rikihisa
The obligatory intracellular pathogen Ehrlichia chaffeensis lacks most genes that confer resistance to oxidative stress but can block reactive oxygen species (ROS) generation by host monocytes-macrophages. Bacterial and host molecules responsible for this inhibition have not been identified. To infect host cells, Ehrlichia uses the C terminus of its surface invasin, entry-triggering protein of Ehrlichia (EtpE; EtpE-C), which directly binds the mammalian cell surface receptor glycosylphosphatidylinositol-anchored protein DNase X...
November 21, 2017: MBio
https://www.readbyqxmd.com/read/29161862/modeling-co-infection-of-ixodes-tick-borne-pathogens
#9
Yijun Lou, Li Liu, Daozhou Gao
Ticks, including the Ixodes ricinus and Ixodes scapularis hard tick species, are regarded as the most common arthropod vectors of both human and animal diseases in Europe and the United States capable of transmitting a large number of bacteria, viruses and parasites. Since ticks in larval and nymphal stages share the same host community which can harbor multiple pathogens, they may be co-infected with two or more pathogens, with a subsequent high likelihood of co-transmission to humans or animals. This paper is devoted to the modeling of co-infection of tick-borne pathogens, with special focus on the co-infection of Borrelia burgdorferi (agent of Lyme disease) and Babesia microti (agent of human babesiosis)...
October 2017: Mathematical Biosciences and Engineering: MBE
https://www.readbyqxmd.com/read/29161493/detection-of-leishmania-v-guyanensis-in-rhipicephalus-boophilus-microplus-acari-ixodidae-collected-from-pecari-tajacu
#10
Jesús E Rojas-Jaimes, Germán H Correa-Nuñez, Nyshon Rojas, Omar Cáceres-Rey
INTRODUCTION: Previous studies identified the presence of Leishmania infantum in Rhipicephalus sanguineus and indicated the possibility that it could transmit leishmaniasis to a variety of hosts. OBJECTIVE: To identify parasites of Leishmania (Viannia) spp. in ticks collected from wild animals in an endemic area for leishmaniasis. MATERIALS AND METHODS: We performed 81 individual DNA extractions from ticks collected from three Tapirus terrestris and three Pecari tajacu in Madre de Dios, Perú...
March 29, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29159591/evaluation-of-the-associations-between-endothelial-dysfunction-inflammation-and-coagulation-in-crimean-congo-hemorrhagic-fever-patients
#11
Halef Okan Doğan, Seyit Ali Büyüktuna, Sercan Kapancik, Sevtap Bakir
Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne zoonotic viral disease. The aim of this study was to evaluate the association between inflammation, coagulation and endothelial dysfunction in CCHF. The study population consisted of 40 patients and 50 healthy controls. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), endocan, high sensitive C-reactive protein (hsCRP), international normalized ratio (INR), prothrombin time (PT), activated partial thromboplastin time (aPTT) and platelets values were determined in blood samples...
November 20, 2017: Archives of Virology
https://www.readbyqxmd.com/read/29159319/co-circulation-of-crimean-congo-hemorrhagic-fever-virus-strains-asia-1-and-2-between-the-border-of-iran-and-pakistan
#12
Nariman Shahhosseini, Ahmad Jafarbekloo, Zakkyeh Telmadarraiy, Sadegh Chinikar, Ali Haeri, Norbert Nowotny, Martin H Groschup, Anthony R Fooks, Faezeh Faghihi
Crimean-Congo Hemorrhagic Fever (CCHF) is a tick-borne viral disease that is transmitted by numerous species of ticks, which serve both as a reservoir and vector of CCHF virus (CCHFV). Molecular and serological tests were undertaken on hard ticks (Ixodidae spp.) and samples from livestock were collected in 2015 from Chabahar County in Southeast Iran. Using RT-PCR, the ticks were tested for the presence of CCHFV. In addition, seven livestock were serologically tested for the presence of IgG antibodies using an ELISA test...
November 2017: Heliyon
https://www.readbyqxmd.com/read/29158428/borrelia-burgdorferi-glycosaminoglycan-binding-protein-bgp-in-the-b31-strain-is-not-essential-for-infectivity-despite-facilitating-adherence-and-tissue-colonization
#13
Samantha Schlachter, Janakiram Seshu, Tao Lin, Steven Norris, Nikhat Parveen
Lyme disease causing Borrelia burgdorferi is transmitted into the mammalian host by an infected tick bite. Successful infection relies on the ability of this extracellular pathogen to persist and colonize different tissues. B. burgdorferi encodes a large number of adhesins that are able to interact with host ligands to facilitate adherence, and tissue colonization. Multiple glycosaminoglycan binding proteins presence in B. burgdorferi offer a degree of redundancy of function during infection, and highlights the importance for glycosaminoglycans as host cell receptors for spirochete adherence...
November 20, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/29157278/ticks-and-the-city-are-there-any-differences-between-city-parks-and-natural-forests-in-terms-of-tick-abundance-and-prevalence-of-spirochaetes
#14
Maciej Kowalec, Tomasz Szewczyk, Renata Welc-Falęciak, Edward Siński, Grzegorz Karbowiak, Anna Bajer
BACKGROUND: Ixodes ricinus ticks are commonly encountered in either natural or urban areas, contributing to Lyme disease agents Borreliella [(Borrelia burgdorferi (sensu lato)] spp. and Borrelia miyamotoi enzootic cycles in cities. It is an actual problem whether urbanization affects pathogen circulation and therefore risk of infection. The aim of the study was to evaluate main tick-borne disease risk factors in natural, endemic areas of north-east (NE) Poland (Białowieża) and urban areas of central Poland (Warsaw), measuring tick abundance/density, prevalence of infection with spirochaetes and diversity of these pathogens in spring-early summer and late summer-autumn periods between 2012 and 2015...
November 21, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29156399/saliva-of-ixodes-ricinus-enhances-tbe-virus-replication-in-dendritic-cells-by-modulation-of-pro-survival-akt-pathway
#15
Jaroslava Lieskovská, Jana Páleníková, Helena Langhansová, Jindřich Chmelař, Jan Kopecký
It has been suggested that tick saliva facilitates transmission of tick-borne encephalitis virus (TBEV) to vertebrates. The mechanism of this facilitation has not been elucidated yet. Since dendritic cells (DCs) are among first cells attacked by the virus, we examined the amount of virus and changes induced by saliva in TBEV-infected DCs. We found that virus replication was significantly increased by saliva of Ixodes ricinus tick. Next, saliva-induced enhancement of Akt pathway activation was observed in TBEV-infected DCs...
November 17, 2017: Virology
https://www.readbyqxmd.com/read/29155863/molecular-detection-of-tick-borne-pathogens-in-cattle-from-southwestern-ethiopia
#16
Zerihun Hailemariam, Jürgen Krücken, Maximilian Baumann, Jabbar S Ahmed, Peter-Henning Clausen, Ard M Nijhof
Tick-borne diseases (TBDs) cause significant losses among livestock and impact the livelihoods of resource-poor farming communities worldwide. In Ethiopia, detailed studies on the epidemiology of tick-borne pathogens (TBPs) in cattle using sensitive molecular detection methods are scarce. The objective of this study was to determine the prevalence and species composition of bovine TBPs of veterinary significance in local cattle populations. A comprehensive cross-sectional epidemiological study was conducted in cattle populations of Illubabor zone in Southwestern Ethiopia from June to August 2013...
2017: PloS One
https://www.readbyqxmd.com/read/29155838/the-tick-biocontrol-agent-metarhizium-brunneum-m-anisopliae-strain-f52-does-not-reduce-non-target-arthropods
#17
Ilya R Fischhoff, Felicia Keesing, Richard S Ostfeld
Previous studies have found that Met52®, which contains the entomopathogenic fungus Metarhizium brunneum, is effective in reducing the abundance of Ixodes scapularis, the tick vector for the bacterium causing Lyme disease and for other tick-borne pathogens. Given widespread interest in effective, safe methods for controlling ticks, Met52 has the potential to be used at increasing scales. The non-target impacts of Met52, as applied for tick control, have not yet been assessed. A Before-After-Control-Impact experiment was conducted to assess the effects of Met52 on non-target arthropods in lawn and forest habitats typical of residential yards...
2017: PloS One
https://www.readbyqxmd.com/read/29155280/molecular-analysis-of-tick-borne-protozoan-and-rickettsial-pathogens-in-small-ruminants-from-two-south-african-provinces
#18
Aaron Edmond Ringo, Paul Franck Adjou Moumouni, Moeti Taioe, Charoonluk Jirapattharasate, Mingming Liu, Guanbo Wang, Yang Gao, Huanping Guo, Seung-Hun Lee, Weiqing Zheng, Artemis Efstratiou, Jixu Li, Noboru Inoue, Hiroshi Suzuki, Oriel Thekisoe, Xuenan Xuan
Tick-borne protozoan and rickettsial diseases are a major threat to livestock in tropical and sub-tropical regions of Africa. In this study we investigated the presence and distribution of Theileria spp., Babesia ovis, Anaplasma ovis, Anaplasma phagocytophilum, Ehrlichia ruminantium and SFG Rickettsia in sheep and goats from Free State and KwaZulu-Natal provinces. A total of 91 blood samples were screened in this study, 61 from goats and 30 from sheep. PCR assay was conducted using primers based on Theileria spp...
November 15, 2017: Parasitology International
https://www.readbyqxmd.com/read/29155165/bovine-babesiosis-cattle-protected-in-the-field-with-a-frozen-vaccine-containing-babesia-bovis-and-babesia-bigemina-cultured-in-vitro-with-a-serum-free-medium
#19
Carmen Rojas-Martínez, Roger Iván Rodríguez-Vivas, Julio Vicente Figueroa Millán, Carlos Ramón Bautista-Garfias, Roberto Omar Castañeda-Arriola, José Juan Lira-Amaya, Patricia Vargas Urióstegui, Juan José Ojeda Carrasco, Jesús Antonio Álvarez Martínez
An attenuated live vaccine containing Babesia bovis and B. bigemina cultured in vitro with a serum-free medium was assessed for its clinical protection conferred of naïve cattle, under natural tick-challenge in a high endemicity zone to Babesia spp. Three groups of six animals were treated as follows: group I (GI) received a vaccine derived from parasites cultured with a free-serum medium; group II (GII) were immunized with the standard vaccine, with parasites cultured in a medium supplemented with 40% (v/v) bovine serum; and a control group (GIII) inoculated with non-infected bovine erythrocytes...
November 16, 2017: Parasitology International
https://www.readbyqxmd.com/read/29153461/population-based-borrelia-burgdorferi-sensu-lato-seroprevalence-and-associated-risk-factors-in-finland
#20
Janko van Beek, Eeva Sajanti, Otto Helve, Jukka Ollgren, Mikko J Virtanen, Harri Rissanen, Outi Lyytikäinen, Jukka Hytönen, Jussi Sane
Lyme borreliosis (LB) is caused by Borrelia burgdorferi sensu lato (Bb-sl) and is the most common vector-borne disease in Europe. The objectives of this study were to determine the Bb-sl seroprevalence among the general Finnish adult population and to identify risk factors associated with Bb-sl-seropositive status. Two thousand sera from a nationwide health survey from 2011 were tested by whole-cell sonicate IgG ELISA, C6 peptide ELISA, and recomBead IgG 2.0 and test results were linked to a general health questionnaire...
November 11, 2017: Ticks and Tick-borne Diseases
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