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mtDNA content oxidative stress

Xianxiu Wan, Jian-Jun Wen, Sue-Jie Koo, Lisa Yi Liang, Nisha Jain Garg
Chronic chagasic cardiomyopathy (CCM) is presented by increased oxidative/inflammatory stress and decreased mitochondrial bioenergetics. SIRT1 senses the redox changes and integrates mitochondrial metabolism and inflammation; and SIRT1 deficiency may be a major determinant in CCM. To test this, C57BL/6 mice were infected with Trypanosoma cruzi (Tc), treated with SIRT1 agonists (resveratrol or SRT1720), and monitored during chronic phase (~150 days post-infection). Resveratrol treatment was partially beneficial in controlling the pathologic processes in Chagas disease...
October 2016: PLoS Pathogens
Lauren M Sparks, Leanne M Redman, Kevin E Conley, Mary-Ellen Harper, Andrew Hodges, Alexey Eroshkin, Sheila R Costford, Meghan E Gabriel, Fanchao Yi, Cherie Shook, Heather H Cornnell, Eric Ravussin, Steven R Smith
CONTEXT: Reduced mitochondrial coupling (P/O) is associated with sedentariness and insulin resistance. Interpreting the physiological relevance of P/O measured in vitro is challenging. OBJECTIVE: To evaluate muscle mitochondrial function and associated transcriptional profiles in non-obese healthy individuals distinguished by their in vivo P/O. DESIGN: Individuals from an ancillary study of CALERIE Phase 2 were assessed at baseline. SETTING: The study was performed at Pennington Biomedical Research Center in Baton Rouge, LA, USA...
October 6, 2016: Journal of Clinical Endocrinology and Metabolism
Rajagopalan Geetha, Chandrasekaran Sathiya Priya, Carani Venkatraman Anuradha
Mitochondrial abnormality is thought to play a key role in cardiac disease originating from the metabolic syndrome (MS). We evaluated the effect of troxerutin (TX), a semi-synthetic derivative of the natural bioflavanoid rutin, on the respiratory chain complex activity, oxidative stress, mitochondrial biogenesis and dynamics in heart of high fat, high fructose diet (HFFD) -induced mouse model of MS. Adult male Mus musculus mice of body weight 25-30 g were fed either control diet or HFFD for 60 days. Mice from each dietary regimen were divided into two groups on the 16(th) day and were treated or untreated with TX (150 mg/Kg bw, p...
September 14, 2016: Clinical and Experimental Pharmacology & Physiology
Jorge L Gamboa, Frederic T Billings, Matthew T Bojanowski, Laura A Gilliam, Chang Yu, Baback Roshanravan, L Jackson Roberts, Jonathan Himmelfarb, T Alp Ikizler, Nancy J Brown
Mitochondria abnormalities in skeletal muscle may contribute to frailty and sarcopenia, commonly present in patients with chronic kidney disease (CKD). Dysfunctional mitochondria are also a major source of oxidative stress and may contribute to cardiovascular disease in CKD We tested the hypothesis that mitochondrial structure and function worsens with the severity of CKD Mitochondrial volume density, mitochondrial DNA (mtDNA) copy number, BNIP3, and PGC1α protein expression were evaluated in skeletal muscle biopsies obtained from 27 subjects (17 controls and 10 with CKD stage 5 on hemodialysis)...
May 2016: Physiological Reports
Zhou Meicen, Zhu Lixin, Cui Xiangli, Feng Linbo, Zhao Xuefeng, He Shuli, Ping Fan, Li Wei, Li Yuxiu
AIMS: To explore the associations between mitochondrial DNA (mtDNA) content and basal plasma glucose, plasma glucose after oral glucose administration, and oxidative stress in a Chinese population with different levels of glucose tolerance. We also aimed to investigate the effect of mtDNA content on basal and oral glucose-stimulated insulin secretion. METHODS: Five hundred and fifty-six Chinese subjects underwent a 75 g, 2 hr Oral Glucose Tolerance Test (OGTT)...
April 22, 2016: Diabetes/metabolism Research and Reviews
G E Carpagnano, D Lacedonia, M Carone, P Soccio, G Cotugno, G A Palmiotti, G Scioscia, M P Foschino Barbaro
Mitochondrial DNA (MtDNA) has been studied as an expression of oxidative stress in asthma, COPD, lung cancer and obstructive sleep apnea, but it has been mainly investigated systemically, although the pathogenetic mechanisms begin in the airways and only later progress to systemic circulation. The aim of this study was to investigate the MtDNA alterations in the exhaled breath condensate (EBC) of patients with asthma, COPD and asthma-COPD overlap syndrome (ACOS). In order to analyze better what happens to mitochondria, both locally and systemically, we compared MtDNA/nDNA in blood and EBC of paired patients...
June 2016: Journal of Breath Research
Yana Konokhova, Sally Spendiff, R Thomas Jagoe, Sudhakar Aare, Sophia Kapchinsky, Norah J MacMillan, Paul Rozakis, Martin Picard, Mylène Aubertin-Leheudre, Charlotte H Pion, Jean Bourbeau, Russell T Hepple, Tanja Taivassalo
BACKGROUND: Low mitochondrial content and oxidative capacity are well-established features of locomotor muscle dysfunction, a prevalent and debilitating systemic occurrence in patients with chronic obstructive pulmonary disease (COPD). Although the exact cause is not firmly established, physical inactivity and oxidative stress are among the proposed underlying mechanisms. Here, we assess the impact of COPD pathophysiology on mitochondrial DNA (mtDNA) integrity, biogenesis, and cellular oxidative capacity in locomotor muscle of COPD patients and healthy controls...
2016: Skeletal Muscle
G E Carpagnano, D Lacedonia, M Malerba, G A Palmiotti, G Cotugno, M Carone, M P Foschino-Barbaro
BACKGROUND: Mitochondria contain their own DNA (MtDNA) that is very sensitive to oxidative stress and as a consequence could be damaged in quantity. Oxidative stress is largely recognized to play a key role in the pathogenesis of asthma and COPD and might have a role in the new intermediate phenotype ACOS (asthma-COPD overlap syndrome). The aim of this study was to investigate MtDNA alterations, as an expression of mitochondrial dysfunction, in ACOS and to verify whether they might help in the identification of this new phenotype and in its differentiation from asthma and COPD...
2016: BMC Pulmonary Medicine
Annalinda Pisano, Bruna Cerbelli, Elena Perli, Maria Pelullo, Valentina Bargelli, Carmela Preziuso, Massimiliano Mancini, Langping He, Matthew G D Bates, Joaquin R Lucena, Paola Lilla Della Monica, Giuseppe Familiari, Vincenzo Petrozza, Chiara Nediani, Robert W Taylor, Giulia d'Amati, Carla Giordano
Mitochondrial (mt) DNA depletion and oxidative mtDNA damage have been implicated in the process of pathological cardiac remodeling. Whether these features are present in the early phase of maladaptive cardiac remodeling, that is, during compensated cardiac hypertrophy, is still unknown. We compared the morphologic and molecular features of mt biogenesis and markers of oxidative stress in human heart from adult subjects with compensated hypertrophic cardiomyopathy and heart failure. We have shown that mtDNA depletion is a constant feature of both conditions...
March 2016: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
A Karimova, Y M Oltulu, H Azaklı, M Kara, D Ustek, B Tutluoglu, I Onaran
In this study we looked at smokers with and without chronic obstructive pulmonary disease (COPD) patients in order to evaluate the incidence of 4977 base pair (bp) mtDNA (mtDNA(4977)) deletion and mtDNA copy number in sputum cells and in peripheral blood leukocytes (PBLs) in relation to mitochondrial function and oxidative stress status. Twenty-five COPD patients who were current smokers, 22 smokers and 23 healthy nonsmokers (for only PBLs studies) participated in this study. The 4977-bp deletion was detected in all examined samples within 40 cyles of PCR amplification, using a quantitative real time PCR...
December 29, 2015: Mitochondrial DNA
Neha Bunkar, Arpit Bhargava, Naveen Kumar Khare, Pradyumna Kumar Mishra
Mitochondria play a fundamental role in regulating a variety of complex metabolic processes to maintain adequate energy balance for cellular existence. To orchestrate these functions, an undisturbed mitochondrial dynamics is imperative through a set of tightly guided mechanisms. Interference in key signature processes by several genetic, epigenetic and age-linked factors triggers mitochondrial dysfunction through decrease in mitochondrial biogenesis, reduced mitochondrial content, aberrant mtDNA mutations, increased oxidative stress, deficient mitophagy, energy dysfunction, decrease in anti oxidant defense and impaired calcium homeostasis...
2016: Frontiers in Bioscience (Landmark Edition)
Judita Knez, Ellen Winckelmans, Michelle Plusquin, Lutgarde Thijs, Nicholas Cauwenberghs, Yumei Gu, Jan A Staessen, Tim S Nawrot, Tatiana Kuznetsova
Accumulation of mitochondrial DNA (mtDNA) mutations leads to alterations of mitochondrial biogenesis and function that might produce a decrease in mtDNA content within cells. This implies that mtDNA content might be a potential biomarker associated with oxidative stress and inflammation. However, data on correlates of mtDNA content in a general population are sparse. Our goal in the present study was to describe in a randomly recruited population sample the distribution and determinants of peripheral blood mtDNA content...
January 15, 2016: American Journal of Epidemiology
Kai Liao, Jing Yan, Kangsen Mai, Qinghui Ai
In response to changes in energy demand and nutrient supply, the organism regulates mitochondrial metabolic status to coordinate ATP production. To survey mitochondrial metabolic adaptation in response to dietary lipid concentration, citrate synthase (EC, CS) activity, the expression of several mitochondrial transcription factors, mitochondrial DNA (mtDNA) copy number, mitochondrial gene expression, mtDNA methylation, and oxidative stress parameters were analyzed in the liver of large yellow croaker fed one of three diets with a low (6%), moderate (12%, the control diet) or high (18%) crude lipid content for 70 d...
March 2016: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Hezhongrong Nie, Guorong Chen, Jing He, Fengjiao Zhang, Ming Li, Qiufeng Wang, Huaibin Zhou, Jianxin Lyu, Yidong Bai
The 4977 bp common deletion is one of the most frequently observed mitochondrial DNA (mtDNA) mutations in human tissues and has been implicated in various human cancer types. It is generally believed that continuous generation of intracellular reactive oxygen species (ROS) during oxidative phosphorylation (OXPHOS) is a major underlying mechanism for generation of such mtDNA deletions while antioxidant systems, including Manganese superoxide dismutase (MnSOD), mitigating the deleterious effects of ROS. However, the clinical significance of this common deletion remains to be explored...
January 2016: Mitochondrion
Wei Ding, Chengyan Xu, Bin Wang, Minmin Zhang
BACKGROUND: Reactive oxygen species (ROS) and inflammation both contribute to the progression of aldosterone-induced renal injury. To better understand the underlying mechanisms, we examined mitochondrial dysfunction and NLRP3 inflammasome activation in aldosterone-infused rats, and explored the role of rotenone in attenuating these injuries. MATERIAL AND METHODS: Sprague-Dawley rats were divided into 3 groups: vehicle-treated, aldosterone-infused, and aldosterone plus rotenone...
2015: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Tao Li, Gui-Lan Chen, Huan Lan, Liang Mao, Bo Zeng
Mitochondrial DNA (mtDNA) deletions are found in many diseased tissues and lead to impairment of mitochondrial functions. In this study, we found wide presence of the common 4977-bp and a novel 4408-bp deletion in the mtDNA of mesenteric arteries from patients with colorectal cancer. These two deletions were also detected in samples from healthy individuals. The content of mtDNA with the 4977-bp deletion was significantly lower in healthy controls than cancer-associated samples, and there was no significant difference for the 4408-bp deletion between the two groups...
September 2016: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
Tamires Santos, Graziela Cancian, Daniella N R Neodini, Deiviston R S Mano, Cristina Capucho, Fabrícia S Predes, Renata Barbieri, Camila A Oliveira, Acácio A Pigoso, Heidi Dolder, Grasiela D C Severi-Aguiar
São Paulo state, Brazil, is one of the main areas of sugar cane planting in the world. Extensive use of ametryn, a triazine herbicide, in sugar cane agriculture and the properties of this herbicide suggest it could be present in the environment as a potential contaminant of soil, surface water, groundwater, and river sediment. In order to clarify the mechanism through which ametryn could be toxic, an in vivo study with Wistar rats was conducted using hematological, biochemical, molecular, morphological and genotoxic approaches...
October 2015: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
C Wang, Y Niu, D Chi, Y Zeng, H Liu, Y Dai, J Li
This study was designed not only to measure the effect of delipation on the developmental viability of pig parthenogenetically activated (PA) embryos, but also to evaluate the changes of mitochondria DNA (mtDNA), reactive oxygen species (ROS) level, adenosine triphosphate (ATP) content and gene (Acsl3, Acadsb, Acaa2, Glut1) expression level at different stages after delipation. Results showed that no effect was observed on the cleavage ability, but significant lower blastocyst rate was obtained in delipated embryos...
October 2015: Reproduction in Domestic Animals, Zuchthygiene
Alan Diot, Alex Hinks-Roberts, Tiffany Lodge, Chunyan Liao, Eszter Dombi, Karl Morten, Stefen Brady, Carl Fratter, Janet Carver, Rebecca Muir, Ryan Davis, Charlotte J Green, Iain Johnston, David Hilton-Jones, Carolyn Sue, Heather Mortiboys, Joanna Poulton
Mitophagy is a cellular mechanism for the recycling of mitochondrial fragments. This process is able to improve mitochondrial DNA (mtDNA) quality in heteroplasmic mtDNA disease, in which mutant mtDNA co-exists with normal mtDNA. In disorders where the load of mutant mtDNA determines disease severity it is likely to be an important determinant of disease progression. Measuring mitophagy is technically demanding. We used pharmacological modulators of autophagy to validate two techniques for quantifying mitophagy...
October 2015: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
M B Betancor, P F Almaida-Pagán, A Hernández, D R Tocher
Mitochondrial decay is generally associated with impairment in the organelle bioenergetics function and increased oxidative stress, and it appears that deterioration of mitochondrial inner membrane phospholipids (PL) and accumulation of mitochondrial DNA (mtDNA) mutations are among the main mechanisms involved in this process. In the present study, mitochondrial membrane PL compositions, oxidative status (TBARS content and SOD activity) and mtDNA gene expression of muscle and liver were analyzed in zebrafish fed two diets with lipid supplied either by rapeseed oil (RO) or a blend 60:40 of RO and DHA500 TG oil (DHA)...
October 2015: Fish Physiology and Biochemistry
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