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Kwanghun Chung

Young Sup Woo, Won-Myong Bahk, Young-Min Park, Sangkeun Chung, Bo-Hyun Yoon, Seunghee Won, Jeong Goo Lee, Hwang-Bin Lee, Won Kim, Jong-Hyun Jeong, Kwanghun Lee, Moon-Doo Kim
We evaluated the effectiveness of aripiprazole among bipolar patients who had switched to this medication as a result of difficulty maintaining on their prestudy atypical antipsychotics (AAPs) because of subsyndromal mood symptoms or intolerance. This study included 77 bipolar patients who were in syndromal remission with an AAP as monotherapy or with an AAP combined with a mood stabilizer(s) who needed to switch from their present AAP because of subsyndromal symptoms or intolerance. At 24 weeks after switching to aripiprazole, the remission rates on the Montgomery-Åsberg Depression Rating Scale (MADRS) and on both the MADRS and the Young Mania Rating Scale were increased significantly in the full sample and in the inefficacy subgroup...
September 2016: International Clinical Psychopharmacology
Taeyun Ku, Justin Swaney, Jeong-Yoon Park, Alexandre Albanese, Evan Murray, Jae Hun Cho, Young-Gyun Park, Vamsi Mangena, Jiapei Chen, Kwanghun Chung
The biology of multicellular organisms is coordinated across multiple size scales, from the subnanoscale of molecules to the macroscale, tissue-wide interconnectivity of cell populations. Here we introduce a method for super-resolution imaging of the multiscale organization of intact tissues. The method, called magnified analysis of the proteome (MAP), linearly expands entire organs fourfold while preserving their overall architecture and three-dimensional proteome organization. MAP is based on the observation that preventing crosslinking within and between endogenous proteins during hydrogel-tissue hybridization allows for natural expansion upon protein denaturation and dissociation...
September 2016: Nature Biotechnology
Young-Eun Jung, Moon-Doo Kim, Won-Myong Bahk, Young Sup Woo, Jonghun Lee, Sae-Heon Jang, Seunghee Won, Kyung Joon Min, Sangkeun Chung, Young-Joon Kwon, Duk-In Jon, Kwanghun Lee, Bo-Hyun Yoon
BACKGROUND: The Bipolar Depression Rating Scale (BDRS) is a scale for assessment of the clinical characteristics of bipolar depression. The primary aims of this study were to describe the development of the Korean version of the BDRS (K-BDRS) and to establish more firmly its psychometric properties in terms of reliability and validity. METHODS: The study included 141 patients (62 male and 79 female) who had been diagnosed with bipolar disorder, were currently experiencing symptoms of depression, and were interviewed using the K-BDRS...
2016: BMC Psychiatry
Alexandre Albanese, Kwanghun Chung
Advances in microscopy and sample preparation have led to the first ever mapping of individual neurons in the whole mouse brain.
January 20, 2016: ELife
Evan Murray, Jae Hun Cho, Daniel Goodwin, Taeyun Ku, Justin Swaney, Sung-Yon Kim, Heejin Choi, Young-Gyun Park, Jeong-Yoon Park, Austin Hubbert, Margaret McCue, Sara Vassallo, Naveed Bakh, Matthew P Frosch, Van J Wedeen, H Sebastian Seung, Kwanghun Chung
Combined measurement of diverse molecular and anatomical traits that span multiple levels remains a major challenge in biology. Here, we introduce a simple method that enables proteomic imaging for scalable, integrated, high-dimensional phenotyping of both animal tissues and human clinical samples. This method, termed SWITCH, uniformly secures tissue architecture, native biomolecules, and antigenicity across an entire system by synchronizing the tissue preservation reaction. The heat- and chemical-resistant nature of the resulting framework permits multiple rounds (>20) of relabeling...
December 3, 2015: Cell
Sung-Yon Kim, Jae Hun Cho, Evan Murray, Naveed Bakh, Heejin Choi, Kimberly Ohn, Luzdary Ruelas, Austin Hubbert, Meg McCue, Sara L Vassallo, Philipp J Keller, Kwanghun Chung
Nondestructive chemical processing of porous samples such as fixed biological tissues typically relies on molecular diffusion. Diffusion into a porous structure is a slow process that significantly delays completion of chemical processing. Here, we present a novel electrokinetic method termed stochastic electrotransport for rapid nondestructive processing of porous samples. This method uses a rotational electric field to selectively disperse highly electromobile molecules throughout a porous sample without displacing the low-electromobility molecules that constitute the sample...
November 17, 2015: Proceedings of the National Academy of Sciences of the United States of America
Hoo Rim Song, Young-Joon Kwon, Won-Myong Bahk, Young Sup Woo, Hwang-Bin Lee, Jonghun Lee, Dae-Bo Lee, Sang-Yeol Lee, Moon-Doo Kim, Seunghee Won, Kwanghun Lee, Inki Sohn, Jung Goo Lee, Young-Chul Shin, Sangkeun Chung, Saeheon Jang, Young Myo Jae, Bo-Hyun Yoon
AIMS: We examined prescription patterns in maintenance treatment for recovered bipolar patients and compared these with acute treatments. METHODS: Using retrospective methods, the bipolar patients in clinical recovery (Clinical Global Impression Bipolar Version score ≤ 2 for 6 months) after acute episode were selected. We reviewed differences between prescription patterns at remission and after a maintenance period of at least 6 months. RESULTS: A total of 340 bipolar disorder patients were selected...
January 2016: Psychiatry and Clinical Neurosciences
Randal Burns, William Gray Roncal, Dean Kleissas, Kunal Lillaney, Priya Manavalan, Eric Perlman, Daniel R Berger, Davi D Bock, Kwanghun Chung, Logan Grosenick, Narayanan Kasthuri, Nicholas C Weiler, Karl Deisseroth, Michael Kazhdan, Jeff Lichtman, R Clay Reid, Stephen J Smith, Alexander S Szalay, Joshua T Vogelstein, R Jacob Vogelstein
We describe a scalable database cluster for the spatial analysis and annotation of high-throughput brain imaging data, initially for 3-d electron microscopy image stacks, but for time-series and multi-channel data as well. The system was designed primarily for workloads that build connectomes- neural connectivity maps of the brain-using the parallel execution of computer vision algorithms on high-performance compute clusters. These services and open-science data sets are publicly available at
2013: Scientific and Statistical Database Management: International Conference, SSDBM ...: Proceedings
Sung-Yon Kim, Kwanghun Chung, Karl Deisseroth
Mapping of neural connectivity across the mammalian brain is a daunting and exciting prospect. Current approaches can be divided into three classes: macroscale, focusing on coarse inter-regional connectivity; mesoscale, involving a finer focus on neurons and projections; and microscale, reconstructing full details of all synaptic contacts. It remains to be determined how to bridge the datasets or insights from the different levels of study. Here we review recent light-microscopy-based approaches that may help in integration across scales...
December 2013: Trends in Cognitive Sciences
Kwanghun Chung, Karl Deisseroth
With potential relevance for brain-mapping work, hydrogel-based structures can now be built from within biological tissue to allow subsequent removal of lipids without mechanical disassembly of the tissue. This process creates a tissue-hydrogel hybrid that is physically stable, that preserves fine structure, proteins and nucleic acids, and that is permeable to both visible-spectrum photons and exogenous macromolecules. Here we highlight relevant challenges and opportunities of this approach, especially with regard to integration with complementary methodologies for brain-mapping studies...
June 2013: Nature Methods
Kwanghun Chung, Jenelle Wallace, Sung-Yon Kim, Sandhiya Kalyanasundaram, Aaron S Andalman, Thomas J Davidson, Julie J Mirzabekov, Kelly A Zalocusky, Joanna Mattis, Aleksandra K Denisin, Sally Pak, Hannah Bernstein, Charu Ramakrishnan, Logan Grosenick, Viviana Gradinaru, Karl Deisseroth
Obtaining high-resolution information from a complex system, while maintaining the global perspective needed to understand system function, represents a key challenge in biology. Here we address this challenge with a method (termed CLARITY) for the transformation of intact tissue into a nanoporous hydrogel-hybridized form (crosslinked to a three-dimensional network of hydrophilic polymers) that is fully assembled but optically transparent and macromolecule-permeable. Using mouse brains, we show intact-tissue imaging of long-range projections, local circuit wiring, cellular relationships, subcellular structures, protein complexes, nucleic acids and neurotransmitters...
May 16, 2013: Nature
Jitendra S Kanodia, Yoosik Kim, Raju Tomer, Zia Khan, Kwanghun Chung, John D Storey, Hang Lu, Philipp J Keller, Stanislav Y Shvartsman
A crucial issue in studies of morphogen gradients relates to their range: the distance over which they can act as direct regulators of cell signaling, gene expression and cell differentiation. To address this, we present a straightforward statistical framework that can be used in multiple developmental systems. We illustrate the developed approach by providing a point estimate and confidence interval for the spatial range of the graded distribution of nuclear Dorsal, a transcription factor that controls the dorsoventral pattern of the Drosophila embryo...
November 2011: Development
Kwanghun Chung, Mei Zhan, Jagan Srinivasan, Paul W Sternberg, Emily Gong, Frank C Schroeder, Hang Lu
The nematode Caenorhabditis elegans is an important model organism in genetic research and drug screening because of its relative simplicity, ease of maintenance, amenability to simple genetic manipulation, and relevance to human biology. However, their small size and mobility make nematodes difficult to physically manipulate, particularly with spatial and temporal precision. We have developed a microfluidic device to overcome these challenges and enable fast behavior-based chemical screening in C. elegans...
November 7, 2011: Lab on a Chip
Kwanghun Chung, Catherine A Rivet, Melissa L Kemp, Hang Lu
Stochasticity in gene expression, protein or metabolite levels contributes to cell-cell variations, the analysis of which could lead to a better understanding of cellular processes and drug responses. Current technologies are limited in their throughput, resolution (in space, time, and tracking individual cells instead of population average) and the ability to control cellular environment. A few microfluidic tools have been developed to trap and image cells; however, in most designs available to date, there is a compromise among loading efficiency, speed, the ability to trap single cells, and density or number of trapped cells...
September 15, 2011: Analytical Chemistry
Yoosik Kim, María José Andreu, Bomyi Lim, Kwanghun Chung, Mark Terayama, Gerardo Jiménez, Celeste A Berg, Hang Lu, Stanislav Y Shvartsman
Developing tissues are patterned by coordinated activities of signaling systems, which can be integrated by a regulatory region of a gene that binds multiple transcription factors or by a transcription factor that is modified by multiple enzymes. Based on a combination of genetic and imaging experiments in the early Drosophila embryo, we describe a signal integration mechanism that cannot be reduced to a single gene regulatory element or a single transcription factor. This mechanism relies on an enzymatic network formed by mitogen-activated protein kinase (MAPK) and its substrates...
June 14, 2011: Developmental Cell
Kwanghun Chung, Yoosik Kim, Jitendra S Kanodia, Emily Gong, Stanislav Y Shvartsman, Hang Lu
Quantitative studies of embryogenesis require the ability to monitor pattern formation and morphogenesis in large numbers of embryos, at multiple time points and in diverse genetic backgrounds. We describe a simple approach that greatly facilitates these tasks for Drosophila melanogaster embryos, one of the most advanced models of developmental genetics. Based on passive hydrodynamics, we developed a microfluidic embryo-trap array that can be used to rapidly order and vertically orient hundreds of embryos. We describe the physical principles of the design and used this platform to quantitatively analyze multiple morphogen gradients in the dorsoventral patterning system...
February 2011: Nature Methods
Matthew M Crane, Kwanghun Chung, Jeffrey Stirman, Hang Lu
This paper reviews the technologies that have been invented in the last few years on high-throughput phenotyping, imaging, screening, and related techniques using microfluidics. The review focuses on the technical challenges and how microfluidics can help to solve these existing problems, specifically discussing the applications of microfluidics to multicellular model organisms. The challenges facing this field include handling multicellular organisms in an efficient manner, controlling the microenvironment and precise manipulation of the local conditions to allow the phenotyping, screening, and imaging of the small animals...
June 21, 2010: Lab on a Chip
Kwanghun Chung, Hang Lu
Laser cell kill is an established tool for studying cells' roles during development and behavior, but its use has been limited due to the manual and low-throughput nature. We demonstrate here a technique combining multiplexing microfluidic manipulation of Caenorhabditis elegans and software for image processing and automation, allowing for high-throughput cell ablations.
October 7, 2009: Lab on a Chip
Kwanghun Chung, Hyewon Lee, Hang Lu
We demonstrate a simple image-based method to measure pressure in microsystems using volume displacement of fluorescent particle suspensions. These micro pressure-sensors are composed of two layers with a poly(dimethylsiloxane) (PDMS) membrane in between: the flow layer includes a flow channel and the sensor layer contains a detection channel filled with suspensions of fluorescent particles. The pressure increase in the flow channel deflects the membrane, and this membrane deformation can be quantified by measuring the cross-sectional areas at specific focal planes...
December 7, 2009: Lab on a Chip
Matthew M Crane, Kwanghun Chung, Hang Lu
Most visual screens based on fluorescent markers are currently limited in throughput and accuracy. Here we present the first genetic screen of a mutagenized population of C. elegans in a microfluidic device. Animal handling streamlined by a microfluidic device and intuitive control software enabled the identification of novel mutants and a large screening speed.
January 7, 2009: Lab on a Chip
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