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https://www.readbyqxmd.com/read/29765035/efficacy-of-a-respiratory-syncytial-virus-vaccine-candidate-in-a-maternal-immunization-model
#1
Jorge C G Blanco, Lioubov M Pletneva, Lori McGinnes-Cullen, Raymonde O Otoa, Mira C Patel, Lurds R Fernando, Marina S Boukhvalova, Trudy G Morrison
Respiratory syncytial virus (RSV) is the most common cause of bronchiolitis in infants. Maternal immunization is an option to increase maternal antibody levels and protect infants from infection. Here we assess the efficacy of virus-like particle (VLP) vaccine candidates containing stabilized pre-fusion (pre-F) or post-fusion (post-F) conformations of the RSV F protein and the attachment RSV G protein in a maternal immunization model using cotton rats. VLP vaccines containing RSV F and G proteins strongly boost pre-existing RSV immunity in dams preventing their perinatal drop in immunity...
May 15, 2018: Nature Communications
https://www.readbyqxmd.com/read/29695428/electrostatic-interactions-between-hendra-virus-matrix-proteins-are-required-for-efficient-virus-like-particle-assembly
#2
Yu Chih Liu, Julian Grusovin, Timothy E Adams
Hendra virus (HeV) is a zoonotic paramyxovirus belonging to the genus Henipavirus. HeV is highly pathogenic, and it can cause severe neurological and respiratory illnesses in both humans and animals, with an extremely high mortality rate of up to 70%. Amongst the genes that HeV encodes, the matrix (M) protein forms an integral part of the virion structure and plays critical roles in coordinating viral assembly and budding. Nevertheless, the molecular mechanism of this process is not fully elucidated. Here, we determined the crystal structure of HeV M to 2...
April 25, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29453861/virus-like-particle-engineering-from-rational-design-to-versatile-applications
#3
REVIEW
Xuanwei Ding, Dong Liu, George Booth, Wei Gao, Yuan Lu
As mimicking natural virus structures, virus-like particles (VLPs) have evolved to become a widely accepted technology used for humans which are safe, highly efficacious, and profitable. Several remarkable advantages have been achieved to revolutionize the molecule delivery for diverse applications in nanotechnology, biotechnology, and medicine. Here, the rational structure design, manufacturing process, functionalization strategy, and emerging applications of VLPs is reviewed. The situation and challenges in the VLP engineering, the key development orientation, and future applications have been discussed...
May 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29212932/minor-capsid-protein-l2-polytope-induces-broad-protection-against-oncogenic-and-mucosal-human-papillomaviruses
#4
Somayeh Pouyanfard, Gloria Spagnoli, Lorenzo Bulli, Kathrin Balz, Fan Yang, Caroline Odenwald, Hanna Seitz, Filipe C Mariz, Angelo Bolchi, Simone Ottonello, Martin Müller
The amino terminus of the human papillomavirus (HPV) minor capsid protein L2 contains a major cross-neutralization epitope which provides the basis for the development of a broadly protecting HPV vaccine. A wide range of protection against different HPV types would eliminate one of the major drawbacks of the commercial, L1-based prophylactic vaccines. Previously, we have reported that insertion of the L2 epitope into a scaffold composed of bacterial thioredoxin protein generates a potent antigen inducing comprehensive protection against different animal and human papillomaviruses...
February 15, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29183342/the-ph-stability-of-foot-and-mouth-disease-virus
#5
REVIEW
Hong Yuan, Pinghua Li, Xueqing Ma, Zengjun Lu, Pu Sun, Xingwen Bai, Jing Zhang, Huifang Bao, Yimei Cao, Dong Li, Yuanfang Fu, Yingli Chen, Qifeng Bai, Jie Zhang, Zaixin Liu
ᅟ: This review summarized the molecular determinants of the acid stability of FMDV in order to explore the uncoating mechanism of FMDV and improve the acid stability of vaccines. BACKGROUND: The foot-and-mouth disease virus (FMDV) capsid is highly acid labile and tends to dissociate into pentameric subunits at acidic condition to release viral RNA for initiating virus replication. However, the acid stability of virus capsid is greatly required for the maintenance of intact virion during the process of virus culture and vaccine production...
November 28, 2017: Virology Journal
https://www.readbyqxmd.com/read/29174141/stability-of-virus-like-particles-of-red-spotted-grouper-nervous-necrosis-virus-in-the-aqueous-state-and-the-vaccine-potential-of-lyophilized-particles
#6
Nguyen Thi Lan, Hyoung Jin Kim, Hyun-Ja Han, Deok-Chan Lee, Bo Kyu Kang, Sang Yoon Han, Hyoungjoon Moon, Hong-Jin Kim
Virus-like particles (VLPs) are multi protein complexes mimicking the structural properties of the native virus. The development of freeze-dried formulations of such complex protein structures remains a challenge. Red-spotted grouper nervous necrosis virus (RGNNV) causes mass mortality in fish culture, and RGNNV VLPs have been suggested to be promising vaccine candidates. In the present study, the stability of RGNNV VLPs in the liquid state was investigated over a 4-week period, along with the influence of freeze-drying on VLP stability...
January 2018: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28935566/construction-of-protein-functionalized-virus-like-particles-of-parvovirus-b19
#7
Ismael Bustos-Jaimes, Ricardo Arturo Soto-Román, Isabel Alejandra Gutiérrez-Landa, Josefina Valadez-García, Carla Lizbeth Segovia-Trinidad
Decoration of virus-like particles (VLPs) expands the repertory of functions these particles can display. In the last years, VLPs have successfully been used as scaffolds to present different molecules, frequently through the specific reaction of chemical groups on the surface of the particles, or by protein engineering when the presentation of peptides or proteins is the primary goal. VLPs of parvovirus B19 (B19V), have been previously produced in vitro and its stability and ability to assemble into hybrid particles composed of wild-type and chimeric proteins evidenced their potential as research tools...
September 19, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28916391/zika-virus-structural-biology-and-progress-in-vaccine-development
#8
REVIEW
Hsiao-Han Lin, Bak-Sau Yip, Li-Min Huang, Suh-Chin Wu
The growing number of zika virus (ZIKV) infections plus a 20-fold increase in neonatal microcephaly in newborns in Brazil have raised alarms in many countries regarding the threat to pregnant women. Instances of microcephaly and central nervous system malformations continue to increase in ZIKV outbreak regions. ZIKV is a small enveloped positive-strand RNA virus belonging to the Flavivirus genus of the Flaviviridae family. High-resolution ZIKV structures recently identified by cryo-electron microscopy indicate that the overall ZIKV structure is similar to those of other flaviviruses...
January 2018: Biotechnology Advances
https://www.readbyqxmd.com/read/28876928/md-simulations-of-viruslike-particles-with-supra-cg-solvation-affordable-to-desktop-computers
#9
Matı As R Machado, Humberto C González, Sergio Pantano
Viruses are tremendously efficient molecular devices that optimize the packing of genetic material using a minimalistic number of proteins to form a capsid or envelope that protects them from external threats, being also part of cell recognition, fusion, and budding machineries. Progress in experimental techniques has provided a large number of high-resolution structures of viruses and viruslike particles (VLP), while molecular dynamics simulations may furnish lively and complementary insights on the fundamental forces ruling viral assembly, stability, and dynamics...
October 10, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28720444/characterization-of-a-spray-dried-candidate-hpv-l2-vlp-vaccine-stored-for-multiple-years-at-room-temperature
#10
Julianne Peabody, Pavan Muttil, Bryce Chackerian, Ebenezer Tumban
HPV infections are associated with human cancers. Although three prophylactic vaccines have been approved to protect against HPV infections, the vaccines require cold-chain storage and may not be suitable for third world countries with less developed refrigeration facilities. We previously developed a bacteriophage L2 virus-like particle (VLP)-based candidate vaccine, which elicited broadly protective antibodies against diverse HPV types. Spray-drying of MS2-16L2 VLPs into a dry powder enhanced the stability of these VLPs...
June 2017: Papillomavirus Research
https://www.readbyqxmd.com/read/28604155/the-importance-of-rsv-f-protein-conformation-in-vlps-in-stimulation-of-neutralizing-antibody-titers-in-mice-previously-infected-with-rsv
#11
Lori M Cullen, Madelyn R Schmidt, Trudy G Morrison
Respiratory syncytial virus (RSV) is a significant respiratory pathogen but no vaccine is available. RSV infections present 2 major, unique problems. First, humans can experience repeated infections caused by the same virus sero-group indicating that protective memory responses to RSV infection are defective. Second, most people have been infected with RSV by age 5. Immune responses to these infections, while poorly protective, could impact the effectiveness of a vaccine. The goal of this study was to assess the generation of protective immune responses in mice previously infected with RSV by virus-like particle (VLP) vaccine candidates containing a stabilized pre-fusion form of the RSV F protein or a stabilized post-fusion F protein...
December 2, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28598590/impact-of-denture-cleansing-solution-immersion-on-some-properties-of-different-denture-base-materials-an-in-vitro-study
#12
Ahmad M Al-Thobity, Mohammed Gad, Aws ArRejaie, Talal Alnassar, Khalifa S Al-Khalifa
PURPOSE: To evaluate the effect of several denture cleansing solutions on the color stability, surface roughness, and flexural strength of three denture base materials. MATERIALS AND METHODS: Twenty-seven specimens were prepared using heat-polymerized (HP) denture base material, 27 using autopolymerized (AP) denture base material, and 27 using visible-light-polymerized (VLP) denture base, creating a total of 81 specimens. The specimens were randomly divided into three groups (n = 27): the distilled water group (DWG), Corega group (CG), and Renew group (RG)...
June 9, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/28592541/dimerization-efficiency-of-canine-distemper-virus-matrix-protein-regulates-membrane-budding-activity
#13
Fanny Bringolf, Michael Herren, Marianne Wyss, Beatriz Vidondo, Johannes P Langedijk, Andreas Zurbriggen, Philippe Plattet
Paramyxoviruses rely on the matrix (M) protein to orchestrate viral assembly and budding at the plasma membrane. Although the mechanistic details remain largely unknown, structural data suggested that M dimers and/or higher-order oligomers may facilitate membrane budding. To gain functional insights, we employed a structure-guided mutagenesis approach to investigate the role of canine distemper virus (CDV) M protein self-assembly in membrane-budding activity. Three six-alanine-block (6A-block) mutants with mutations located at strategic oligomeric positions were initially designed...
August 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28578190/correlation-between-dynamic-light-scattering-and-size-exclusion-high-performance-liquid-chromatography-for-monitoring-the-effect-of-ph-on-stability-of-biopharmaceuticals
#14
Medhat A Al-Ghobashy, Moushira M Mostafa, Heba S Abed, Faten A Fathalla, Maissa Y Salem
Aggregate formation is a major problem affecting both safety and efficacy of biopharmaceuticals and is associated with protein immunogenicity. Size exclusion high performance liquid chromatography (SE-HPLC) has always been the gold standard technique for detection and determination of protein aggregates. However, large protein aggregates may be filtered off and build up on top of the column leading to deterioration in column performance. Moreover, low-affinity protein aggregates may dissociate during analysis and thus not detected...
August 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28540352/dataset-on-galanin-receptor-3-mutants-that-improve-recombinant-receptor-expression-and-stability-in-an-agonist-and-antagonist-bound-form
#15
Thao T Ho, Jasmine T Nguyen, Juping Liu, Pawel Stanczak, Aaron A Thompson, Yingzhuo G Yan, Jasmine Chen, Charles K Allerston, Charles L Dillard, Hao Xu, Nicholas J Shoger, Jill S Cameron, Mark E Massari, Kathleen Aertgeerts
Galanin Receptor 3 (GALR3) is a G-protein-coupled receptor with a widespread distribution in the brain and plays a role in a variety of physiologic processes including cognition/memory, sensory/pain processing, hormone secretion, and feeding behavior. Therefore, GALR3 is considered an attractive CNS drug target (Freimann et al., 2015) [1]. This dataset contains GALR3 point mutants that improve recombinant protein expression and thermal stability of the receptor contained in virus-like particles (VLPs) or obtained by detergent-purification of baculovirus-infected insect cells...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28387161/biomechanical-assessment-of-the-dorsal-spanning-bridge-plate-in-distal-radius-fracture-fixation-implications-for-immediate-weight-bearing
#16
Jerry I Huang, Bret Peterson, Kate Bellevue, Nicolas Lee, Sean Smith, Safa Herfat
BACKGROUND: The goal of this study was to compare the biomechanical stability of a 2.4-mm dorsal spanning bridge plate with a volar locking plate (VLP) in a distal radius fracture model, during simulated crutch weight-bearing. METHODS: Five paired cadaveric forearms were tested. A 1-cm dorsal wedge osteotomy was created to simulate an unstable distal radius fracture with dorsal comminution. Fractures were fixed with a VLP or a dorsal bridge plate (DBP). Specimens were mounted to a crutch handle, and optical motion-tracking sensors were attached to the proximal and distal segments...
April 1, 2017: Hand: Official Journal of the American Association for Hand Surgery
https://www.readbyqxmd.com/read/28328521/human-papillomavirus-first-and-second-generation-vaccines-current-status-and-future-directions
#17
REVIEW
Somayeh Pouyanfard, Martin Müller
It has been more than 10 years that the first prophylactic papillomavirus vaccine became available, although distribution has been mainly limited to the more affluent countries. The first two vaccines have been a great success, hundreds of millions of women and a much smaller number of men have been vaccinated ever since. In a few countries with high vaccination coverage, in particular Australia but also parts of Great Britain and others, clinical impact of vaccination programs is already visible and there are indications for herd immunity as well...
July 26, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/28263854/method-for-rapid-optimization-of-recombinant-gpcr-protein-expression-and-stability-using-virus-like-particles
#18
Thao T Ho, Jasmine T Nguyen, Juping Liu, Pawel Stanczak, Aaron A Thompson, Yingzhuo G Yan, Jasmine Chen, Charles K Allerston, Charles L Dillard, Hao Xu, Nicholas J Shoger, Jill S Cameron, Mark E Massari, Kathleen Aertgeerts
Recent innovative approaches to stabilize and crystallize GPCRs have resulted in an unprecedented breakthrough in GPCR crystal structures as well as application of the purified receptor protein in biophysical and biochemical ligand binding assays. However, the protein optimization process to enable these technologies is lengthy and requires iterative overexpression, solubilization, purification and functional analysis of tens to hundreds of protein variants. Here, we report a new and versatile method to screen in parallel hundreds of GPCR variants in HEK293 produced virus-like particles (VLPs) for protein yield, stability, functionality and ligand binding...
May 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28222997/integrated-analysis-of-recombinant-bpv-1-l1-protein-for-the-production-of-a-bovine-papillomavirus-vlp-vaccine
#19
Diego Grando Módolo, Rodrigo Pinheiro Araldi, Jacqueline Mazzuchelli-de-Souza, Alexandre Pereira, Daniel Carvalho Pimenta, Letícia Maria Zanphorlin, Willy Beçak, Marcelo Menossi, Rita de Cassia Stocco, Rodrigo Franco de Carvalho
Bovine papillomatosis is an infectious disease that is caused by bovine papillomavirus (BPV), which results in important economic losses. However, no BPV vaccines or effective treatment methods are commercially available to date. Moreover, the absence of papillomavirus replication in vitro makes the use of recombinant protein a promising candidate for vaccine formulations. Hence, we developed an integrated study on the L1 capsid protein of BPV-1, obtained from a bacterial expression system, regarding its purification, biosafety, thermostability and immunogenicity...
March 14, 2017: Vaccine
https://www.readbyqxmd.com/read/27928008/increasing-type-1-poliovirus-capsid-stability-by-thermal-selection
#20
Oluwapelumi O Adeyemi, Clare Nicol, Nicola J Stonehouse, David J Rowlands
Poliomyelitis is a highly infectious disease caused by poliovirus (PV). It can result in paralysis and may be fatal. Integrated global immunization programs using live-attenuated oral (OPV) and/or inactivated (IPV) PV vaccines have systematically reduced its spread and paved the way for eradication. Immunization will continue posteradication to ensure against reintroduction of the disease, but there are biosafety concerns for both OPV and IPV. They could be addressed by the production and use of virus-free virus-like particle (VLP) vaccines that mimic the "empty" capsids (ECs) normally produced in viral infection...
February 15, 2017: Journal of Virology
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