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https://www.readbyqxmd.com/read/28604155/the-importance-of-rsv-f-protein-conformation-in-vlps-in-stimulation-of-neutralizing-antibody-titers-in-mice-previously-infected-with-rsv
#1
Lori M Cullen, Madelyn R Schmidt, Trudy G Morrison
Respiratory syncytial virus (RSV) is a significant respiratory pathogen but no vaccine is available. RSV infections present 2 major, unique problems. First, humans can experience repeated infections caused by the same virus sero-group indicating that protective memory responses to RSV infection are defective. Second, most people have been infected with RSV by age 5. Immune responses to these infections, while poorly protective, could impact the effectiveness of a vaccine. The goal of this study was to assess the generation of protective immune responses in mice previously infected with RSV by virus-like particle (VLP) vaccine candidates containing a stabilized pre-fusion form of the RSV F protein or a stabilized post-fusion F protein...
June 12, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28598590/impact-of-denture-cleansing-solution-immersion-on-some-properties-of-different-denture-base-materials-an-in-vitro-study
#2
Ahmad M Al-Thobity, Mohammed Gad, Aws ArRejaie, Talal Alnassar, Khalifa S Al-Khalifa
PURPOSE: To evaluate the effect of several denture cleansing solutions on the color stability, surface roughness, and flexural strength of three denture base materials. MATERIALS AND METHODS: Twenty-seven specimens were prepared using heat-polymerized (HP) denture base material, 27 using autopolymerized (AP) denture base material, and 27 using visible-light-polymerized (VLP) denture base, creating a total of 81 specimens. The specimens were randomly divided into three groups (n = 27): the distilled water group (DWG), Corega group (CG), and Renew group (RG)...
June 9, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/28592541/dimerization-efficiency-of-canine-distemper-virus-matrix-protein-regulates-membrane-budding-activity
#3
Fanny Bringolf, Michael Herren, Marianne Wyss, Beatriz Vidondo, Johannes P Langedijk, Andreas Zurbriggen, Philippe Plattet
Paramyxoviruses rely on the matrix (M) protein to orchestrate viral assembly and budding at the plasma membrane. Although mechanistic details remain largely unknown, structural data suggested that M dimers and/or higher oligomers may facilitate membrane budding. To gain functional insights, we here employed a structure-guided mutagenesis approach to investigate the role of canine distemper virus (CDV) M protein self-assembly in membrane budding activity. Three six-alanine-scan (6A) block mutants, locating at strategic oligomeric positions, were initially designed...
June 7, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28578190/correlation-between-dynamic-light-scattering-and-size-exclusion-high-performance-liquid-chromatography-for-monitoring-the-effect-of-ph-on-stability-of-biopharmaceuticals
#4
Medhat A Al-Ghobashy, Moushira M Mostafa, Heba S Abed, Faten A Fathalla, Maissa Y Salem
Aggregate formation is a major problem affecting both safety and efficacy of biopharmaceuticals and is associated with protein immunogenicity. Size exclusion high performance liquid chromatography (SE-HPLC) has always been the gold standard technique for detection and determination of protein aggregates. However, large protein aggregates may be filtered off and build up on top of the column leading to deterioration in column performance. Moreover, low-affinity protein aggregates may dissociate during analysis and thus not detected...
May 26, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28540352/dataset-on-galanin-receptor-3-mutants-that-improve-recombinant-receptor-expression-and-stability-in-an-agonist-and-antagonist-bound-form
#5
Thao T Ho, Jasmine T Nguyen, Juping Liu, Pawel Stanczak, Aaron A Thompson, Yingzhuo G Yan, Jasmine Chen, Charles K Allerston, Charles L Dillard, Hao Xu, Nicholas J Shoger, Jill S Cameron, Mark E Massari, Kathleen Aertgeerts
Galanin Receptor 3 (GALR3) is a G-protein-coupled receptor with a widespread distribution in the brain and plays a role in a variety of physiologic processes including cognition/memory, sensory/pain processing, hormone secretion, and feeding behavior. Therefore, GALR3 is considered an attractive CNS drug target (Freimann et al., 2015) [1]. This dataset contains GALR3 point mutants that improve recombinant protein expression and thermal stability of the receptor contained in virus-like particles (VLPs) or obtained by detergent-purification of baculovirus-infected insect cells...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28387161/biomechanical-assessment-of-the-dorsal-spanning-bridge-plate-in-distal-radius-fracture-fixation-implications-for-immediate-weight-bearing
#6
Jerry I Huang, Bret Peterson, Kate Bellevue, Nicolas Lee, Sean Smith, Safa Herfat
BACKGROUND: The goal of this study was to compare the biomechanical stability of a 2.4-mm dorsal spanning bridge plate with a volar locking plate (VLP) in a distal radius fracture model, during simulated crutch weight-bearing. METHODS: Five paired cadaveric forearms were tested. A 1-cm dorsal wedge osteotomy was created to simulate an unstable distal radius fracture with dorsal comminution. Fractures were fixed with a VLP or a dorsal bridge plate (DBP). Specimens were mounted to a crutch handle, and optical motion-tracking sensors were attached to the proximal and distal segments...
April 1, 2017: Hand: Official Journal of the American Association for Hand Surgery
https://www.readbyqxmd.com/read/28328521/human-papillomavirus-first-and-second-generation-vaccines-current-status-and-future-directions
#7
Somayeh Pouyanfard, Martin Müller
It has been more than 10 years that the first prophylactic papillomavirus vaccine became available, although distribution has been mainly limited to the more affluent countries. The first two vaccines have been a great success, hundreds of millions of women and a much smaller number of men have been vaccinated ever since. In a few countries with high vaccination coverage, in particular Australia but also parts of Great Britain and others, clinical impact of vaccination programs is already visible and there are indications for herd immunity as well...
March 22, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/28263854/method-for-rapid-optimization-of-recombinant-gpcr-protein-expression-and-stability-using-virus-like-particles
#8
Thao T Ho, Jasmine T Nguyen, Juping Liu, Pawel Stanczak, Aaron A Thompson, Yingzhuo G Yan, Jasmine Chen, Charles K Allerston, Charles L Dillard, Hao Xu, Nicholas J Shoger, Jill S Cameron, Mark E Massari, Kathleen Aertgeerts
Recent innovative approaches to stabilize and crystallize GPCRs have resulted in an unprecedented breakthrough in GPCR crystal structures as well as application of the purified receptor protein in biophysical and biochemical ligand binding assays. However, the protein optimization process to enable these technologies is lengthy and requires iterative overexpression, solubilization, purification and functional analysis of tens to hundreds of protein variants. Here, we report a new and versatile method to screen in parallel hundreds of GPCR variants in HEK293 produced virus-like particles (VLPs) for protein yield, stability, functionality and ligand binding...
March 3, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28222997/integrated-analysis-of-recombinant-bpv-1-l1-protein-for-the-production-of-a-bovine-papillomavirus-vlp-vaccine
#9
Diego Grando Módolo, Rodrigo Pinheiro Araldi, Jacqueline Mazzuchelli-de-Souza, Alexandre Pereira, Daniel Carvalho Pimenta, Letícia Maria Zanphorlin, Willy Beçak, Marcelo Menossi, Rita de Cassia Stocco, Rodrigo Franco de Carvalho
Bovine papillomatosis is an infectious disease that is caused by bovine papillomavirus (BPV), which results in important economic losses. However, no BPV vaccines or effective treatment methods are commercially available to date. Moreover, the absence of papillomavirus replication in vitro makes the use of recombinant protein a promising candidate for vaccine formulations. Hence, we developed an integrated study on the L1 capsid protein of BPV-1, obtained from a bacterial expression system, regarding its purification, biosafety, thermostability and immunogenicity...
February 17, 2017: Vaccine
https://www.readbyqxmd.com/read/27928008/increasing-type-1-poliovirus-capsid-stability-by-thermal-selection
#10
Oluwapelumi O Adeyemi, Clare Nicol, Nicola J Stonehouse, David J Rowlands
Poliomyelitis is a highly infectious disease caused by poliovirus (PV). It can result in paralysis and may be fatal. Integrated global immunization programs using live-attenuated oral (OPV) and/or inactivated (IPV) PV vaccines have systematically reduced its spread and paved the way for eradication. Immunization will continue posteradication to ensure against reintroduction of the disease, but there are biosafety concerns for both OPV and IPV. They could be addressed by the production and use of virus-free virus-like particle (VLP) vaccines that mimic the "empty" capsids (ECs) normally produced in viral infection...
February 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/27920180/whole-inactivated-and-virus-like-particle-vaccine-strategies-for-chikungunya-virus
#11
Adam D DeZure, Nina M Berkowitz, Barney S Graham, Julie E Ledgerwood
Chikungunya virus (CHIKV) is a global public health threat, having been identified in >60 countries in Asia, Africa, Europe, and the Americas. There is no cure for or licensed vaccine against CHIKV infection. Initial attempts at CHIKV vaccine development began in the early 1960s. Whole-inactivated and virus-like particle (VLP) vaccines are 2 of the current approaches being evaluated. Success of these approaches is dependent on a safe, well-tolerated vaccine that is immunogenic and deployable in regard to manufacturing, stability, and delivery characteristics...
December 15, 2016: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27903913/functional-rnas-combined-assembly-and-packaging-in-vlps
#12
Po-Yu Fang, Lizzette M Gómez Ramos, Stefany Y Holguin, Chiaolong Hsiao, Jessica C Bowman, Hung-Wei Yang, Loren Dean Williams
We describe here a one pot RNA production, packaging and delivery system based on bacteriophage Qβ. We demonstrate a method for production of a novel RNAi scaffold, packaged within Qβ virus-like particles (VLPs). The RNAi scaffold is a general utility chimera that contains a functional RNA duplex with paired silencing and carrier sequences stabilized by a miR-30 stem-loop. The Qβ hairpin on the 5΄ end confers affinity for the Qβ coat protein (CP). Silencing sequences can include mature miRNAs and siRNAs, and can target essentially any desired mRNA...
April 7, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/27793484/real-time-stability-of-a-hepatitis-e-vaccine-hecolin%C3%A2-demonstrated-with-potency-assays-and-multifaceted-physicochemical-methods
#13
Xiao Zhang, Minxi Wei, Guang Sun, Xin Wang, Min Li, Zhijie Lin, Zhongyi Li, Yufang Li, Mujin Fang, Jun Zhang, Shaowei Li, Ningshao Xia, Qinjian Zhao
The first prophylactic vaccine against hepatitis E virus (HEV), Hecolin®, was licensed in China. Recombinant p239 virus-like particle (VLP) is its active component with dimeric protein as the basic building block harboring the immuno dominant and neutralizing epitopes. The real time and real condition stability of the prefilled syringes for the vaccine was demonstrated using both in vivo mouse potency and in vitro antigenicity assays. A total of 12 lots of Hecolin® were assessed with a set of assays after storage at 2-8°C for 24months...
October 25, 2016: Vaccine
https://www.readbyqxmd.com/read/27556288/tuning-viral-capsid-nanoparticle-stability-with-symmetrical-morphogenesis
#14
Aida Llauró, Benjamin Schwarz, Ranjit Koliyatt, Pedro J de Pablo, Trevor Douglas
Virus-like particles (VLPs) provide engineering platforms for the design and implementation of protein-based nanostructures. These capsids are comprised of protein subunits whose precise arrangement and mutual interactions determine their stability, responsiveness to destabilizing environments, and ability to undergo morphological transitions. The precise interplay between subunit contacts and the overall stability of the bulk capsid population remains poorly resolved. Approaching this relationship requires a combination of techniques capable of accessing nanoscale properties, such as the mechanics of individual capsids, and bulk biochemical procedures capable of interrogating the stability of the VLP ensemble...
September 27, 2016: ACS Nano
https://www.readbyqxmd.com/read/27414779/-vlp-vaccines-and-effects-of-hiv-1-env-protein-modifications-on-their-antigenic-properties
#15
REVIEW
A N Vzorov, R W Compans
An ideal protective HIV-1 vaccine can elicit broadly neutralizing antibodies, capable of preventing HIV transmission. The strategies of designing vaccines include generation of soluble recombinant proteins which mimic the native Env complex and are able to enhance the immunogenicity of gp120. Recent data indicate that the cytoplasmic tail (CT) of the Env protein has multiple functions, which can affect the early steps of infection, as well as viral assembly and antigenic properties. Modifications in the CT can be used to induce conformational changes in functional regions of gp120 and to stabilize the trimeric structure, avoiding immune misdirection and induction of non-neutralizing antibody responses...
May 2016: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/27377328/quinoxaline-based-inhibitors-of-ebola-and-marburg-vp40-egress
#16
H Marie Loughran, Ziying Han, Jay E Wrobel, Sarah E Decker, Gordon Ruthel, Bruce D Freedman, Ronald N Harty, Allen B Reitz
We prepared a series of quinoxalin-2-mercapto-acetyl-urea analogs and evaluated them for their ability to inhibit viral egress in our Marburg and Ebola VP40 VLP budding assays in HEK293T cells. We also evaluated selected compounds in our bimolecular complementation assay (BiMC) to detect and visualize a Marburg mVP40-Nedd4 interaction in live mammalian cells. Antiviral activity was assessed for selected compounds using a live recombinant vesicular stomatitis virus (VSV) (M40 virus) that expresses the EBOV VP40 PPxY L-domain...
August 1, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27351167/dual-functionalized-bacteriophage-q%C3%AE-as-a-photocaged-drug-carrier
#17
Zhuo Chen, Na Li, Luxi Chen, Jiyong Lee, Jeremiah J Gassensmith
Proteinatious nanoparticles are emerging as promising materials in biomedical research owing to their many unique properties and our interest focuses on integrating environmental responsivity into these systems. In this work, the use of a virus-like particle (VLP) derived from bacteriophage Qβ as a photocaged drug delivery system is investigated. Ideally, a photocaged nanoparticle platform should be harmless and inert without activation by light yet, upon photoirradiation, should cause cell death. Approximately 530 photocleavable doxorubicin complexes are installed initially onto the surface of Qβ by CuAAC reaction for photocaging therapy; however, aggregation and precipitation are found to cause cell death at higher concentrations...
September 2016: Small
https://www.readbyqxmd.com/read/27286040/expression-and-purification-of-virus-like-particles-for-vaccination
#18
Maria T Arevalo, Terianne M Wong, Ted M Ross
Virus-like particles (VLPs) and subviral particles (SVPs) are an alternative approach to viral vaccine design that offers the advantages of increased biosafety and stability over use of live pathogens. Non-infectious and self-assembling, VLPs are used to present structural proteins as immunogens, bypassing the need for live pathogens or recombinant viral vectors for antigen delivery. In this article, we demonstrate the different stages of VLP design and development for future applications in preclinical animal testing...
June 2, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27259072/using-a-visible-light-polymerized-resin-to-fabricate-an-interim-partial-removable-dental-prosthesis
#19
Aaron Segal, Hui Wen Yu, Heba Elkassaby
An interim partial removable dental prosthesis (RDP) is any dental prosthesis that replaces some teeth in a partially dentate arch designed to enhance esthetics, stabilization, and/or function for a limited period of time, after which it is to be replaced by a definitive dental prosthesis. This article describes a technique that uses a visible light-polymerized (VLP) resin as the base material for an interim partial RDP. This technique can be easily accomplished in a dental office or laboratory and results in a predictable dental prosthesis...
June 3, 2016: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/27231578/the-hemifusion-structure-induced-by-influenza-virus-haemagglutinin-is-determined-by-physical-properties-of-the-target-membranes
#20
Petr Chlanda, Elena Mekhedov, Hang Waters, Cindi L Schwartz, Elizabeth R Fischer, Rolf J Ryham, Fredric S Cohen, Paul S Blank, Joshua Zimmerberg
Influenza A virus hemagglutinin (HA) changes conformation and drives membrane fusion of viral and endosomal membrane at low pH. Membrane fusion proceeds through an intermediate called hemifusion(1,2). For viral fusion the hemifusion structures are not determined(3). Here, influenza virus-like particles (VLP)(4) carrying wild-type (WT) HA or HA hemifusion mutant G1S(5) and liposome mixtures were studied at low pH by Volta phase plate (VPP) cryo-electron tomography (cET) which improves signal-to-noise ratio close to focus...
2016: Nature Microbiology
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