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https://www.readbyqxmd.com/read/27933682/high-efficient-intramolecular-electrochemiluminescence-energy-transfer-for-ultrasensitive-bioanalysis-of-aflatoxin-m1
#1
Jiali Liu, Min Zhao, Ying Zhuo, Yaqin Chai, Ruo Yuan
The intermolecular electrochemiluminescence resonance energy transfer (ECL-RET) between luminol and Ru(bpy)32+ was studied extensively to achieve the sensitive bio-analysis owing to the perfect spectral overlap of the donor and acceptor, but it still suffers from the challenging issue of low energy transfer efficiency. Here, the intramolecular ECL-RET towards the novel ECL compound containing the donor of luminol and the acceptor of Ru(bpy)2(mcbpy)2+ was designed and investigated for the first time (The compound was abbreviated as Lum-Ru)...
December 8, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27933645/aflatoxin-biomarkers-in-hair-may-facilitate-long-term-exposure-studies
#2
Innocent Mupunga, Christo D Izaaks, Leshweni J Shai, David R Katerere
Aflatoxins are highly toxic fungal metabolites produced by some members of the Aspergillus species. They are low molecular weight lipophilic compounds that are easily absorbed from the gastrointestinal tract. They contaminate most staple foods, including maize, peanuts, peanut butter and sorghum mainly in the tropics where hot and humid conditions promote fungal growth. Absorbed aflatoxins are metabolized by the cytochrome P450 enzyme system in the liver into toxic metabolites. Aflatoxin B (AFB)1 is the most toxic, carcinogenic and mutagenic naturally occurring toxin...
December 9, 2016: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/27933586/preparation-and-optimization-of-meropenem-loaded-solid-lipid-nanoparticles-in-vitro-evaluation-and-molecular-modeling
#3
Ellen K G Mhango, Rahul S Kalhapure, Mahantesh Jadhav, Sandeep J Sonawane, Chunderika Mocktar, Suresh Vepuri, Mahmoud Soliman, Thirumala Govender
Encapsulation of antibiotics into nanocarriers has the potential to overcome resistance and disadvantages associated with conventional dosage forms as well as increase half-life of an antibiotic. Encapsulation of meropenem (MRPN) into solid lipid nanoparticles (SLNs) remains unexplored among the limited work reported on nanoformulation incorporating MRPN. The study aimed to use an experimental design, to optimize MRPN-loaded SLNs, and to undertake in vitro and in silico evaluations. A Box-Behnken design (BBD) was used to optimize manufacturing conditions of glycerol monostearate (GMS) SLNs loaded with MRPN...
December 8, 2016: AAPS PharmSciTech
https://www.readbyqxmd.com/read/27933097/molecular-mechanisms-of-hpv-mediated-neoplastic-progression
#4
REVIEW
Rashmirani Senapati, Nihar Nalini Senapati, Bhagirathi Dwibedi
Human Papillomavirus is the major etiological agent in the development of cervical cancer but not a sufficient cause. Despite significant research, the underlying mechanisms of progression from a low-grade squamous intraepithelial lesion to high grade squamous intraepithelial lesion are yet to be understood. Deregulation of viral gene expression and host genomic instability play a central role in virus-mediated carcinogenesis. Key events such as viral integration and epigenetic modifications may lead to the deregulation of viral and host gene expression...
2016: Infectious Agents and Cancer
https://www.readbyqxmd.com/read/27932949/muscle-type-nicotinic-receptor-modulation-by-2-6-dimethylaniline-a-molecule-resembling-the-hydrophobic-moiety-of-lidocaine
#5
Armando Alberola-Die, Gregorio Fernández-Ballester, José M González-Ros, Isabel Ivorra, Andrés Morales
To identify the molecular determinants responsible for lidocaine blockade of muscle-type nAChRs, we have studied the effects on this receptor of 2,6-dimethylaniline (DMA), which resembles lidocaine's hydrophobic moiety. Torpedo marmorata nAChRs were microtransplanted to Xenopus oocytes and currents elicited by ACh (IACh), either alone or co-applied with DMA, were recorded. DMA reversibly blocked IACh and, similarly to lidocaine, exerted a closed-channel blockade, as evidenced by the enhancement of IACh blockade when DMA was pre-applied before its co-application with ACh, and hastened IACh decay...
2016: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/27932562/intraoperative-assessment-of-tumor-resection-margins-in-breast-conserving-surgery-using-18f-fdg-cerenkov-luminescence-imaging-a-first-in-human-feasibility-study
#6
Maarten Ruben Grootendorst, Massimiliano Cariati, Sarah Pinder, Ashutosh Kothari, Michael Douek, Tibor Kovacs, Hisham Hamed, Amit Pawa, Fiona Nimmo, Julie Owen, Vernie Ramalingam, Sweta Sethi, Sanjay Mistry, Kunal Vyas, David Tuch, Alan Britten, Mieke Van Hemelrijck, Gary Cook, Chris Sibley-Allen, Sarah Allen, Arnie Purushotham
RATIONALE: In early-stage breast cancer, the primary treatment option for the majority of women is breast-conserving surgery (BCS). There is a clear need for more accurate techniques to assess resection margins intraoperatively, as on average 20% of patients require further surgery to achieve clear margins. Cerenkov luminescence imaging (CLI) combines optical and molecular imaging by detecting light emitted by (18)F-fluorodeoxyglucose ((18)F-FDG). Its high-resolution and small size imaging equipment makes CLI a promising technology for intraoperative margin assessment...
December 8, 2016: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/27932355/familial-hypercholesterolemia-phenotype-in-chinese-patients-undergoing-coronary-angiography
#7
Jian-Jun Li, Sha Li, Cheng-Gang Zhu, Na-Qiong Wu, Yan Zhang, Yuan-Lin Guo, Ying Gao, Xiao-Lin Li, Ping Qing, Chuan-Jue Cui, Rui-Xia Xu, Zheng-Wen Jiang, Jing Sun, Geng Liu, Qian Dong
OBJECTIVE: Familial hypercholesterolemia (FH) is characterized by an elevated low-density lipoprotein cholesterol and increased risk of premature coronary artery disease. However, the general picture and mutational spectrum of FH in China are far from recognized, representing a missed opportunity for the investigation. APPROACH AND RESULTS: A total of 8050 patients undergoing coronary angiography were enrolled. The diagnosis of clinical FH was made using Dutch Lipid Clinic Network criteria, and the information of relatives was obtained by inquiring for the probands or from their own medical records of certain clinics/hospitals...
December 8, 2016: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/27932253/regulation-of-cyclin-d1-by-arsenic-and-microrna-inhibits-adipogenesis
#8
Kevin Beezhold, Linda R Klei, Aaron Barchowsky
Low-dose chronic exposure to arsenic in drinking water represents a global public health concern with established risks for metabolic and cardiovascular disease, as well as cancer. While the linkage between arsenic and disease is strong, further understanding of the molecular mechanisms of its pathogenicity is required. Previous reports demonstrated the ability of arsenic to interfere with adipogenesis, which may mediate its effects in promoting metabolic disease. We hypothesized that microRNA are important regulators of most if not all mesenchymal stem cell processes that are dysregulated by arsenic exposure to impair lipogenesis...
December 5, 2016: Toxicology Letters
https://www.readbyqxmd.com/read/27932040/embryonic-exposure-to-model-naphthenic-acids-delays-growth-and-hatching-in-the-pond-snail-lymnaea-stagnalis
#9
Christina U Johnston, Lindsay N Clothier, Dean M Quesnel, Lisa M Gieg, Gordon Chua, Petra M Hermann, Willem C Wildering
Naphthenic acids (NAs), a class of structurally diverse carboxylic acids with often complex ring structures and large aliphatic tail groups, are important by-products of many petrochemical processes including the oil sands mining activity of Northern Alberta. While it is evident that NAs have both acute and chronic harmful effects on many organisms, many aspects of their toxicity remain to be clarified. Particularly, while substantive data sets have been collected on NA toxicity in aquatic prokaryote and vertebrate model systems, to date, nothing is known about the toxic effects of these compounds on the embryonic development of aquatic invertebrate taxa, including freshwater mollusks...
December 5, 2016: Chemosphere
https://www.readbyqxmd.com/read/27931018/primary-carnitine-deficiency-and-newborn-screening-for-disorders-of-the-carnitine-cycle
#10
Nicola Longo
Carnitine is needed for transfer of long-chain fatty acids across the inner mitochondrial membrane for subsequent β-oxidation. Carnitine can be synthesized by the body and is also obtained in the diet through consumption of meat and dairy products. Defects in carnitine transport such as those caused by defective activity of the OCTN2 transporter encoded by the SLC22A5 gene result in primary carnitine deficiency, and newborn screening programmes can identify patients at risk for this condition before irreversible damage...
December 9, 2016: Annals of Nutrition & Metabolism
https://www.readbyqxmd.com/read/27930960/loss-of-rearranged-l-myc-fusion-rlf-results-in-defects-in-heart-development-in-the-mouse
#11
L M Bourke, G Del Monte-Nieto, J E Outhwaite, V Bharti, P M Pollock, D G Simmons, A Adam, S S J Hur, G J Maghzal, E Whitelaw, R Stocker, C M Suter, R P Harvey, S K Harten
Recently we reported that Rearranged L-Myc Fusion, RLF, acts as an epigenetic modifier maintaining low levels of DNA methylation at CpG island shores and enhancers across the genome. Here we focus on the phenotype of Rlf null mutant mice generated via an ENU mutagenesis screen, to identify genes required for epigenetic regulation. RLF is expressed in a range of fetal mouse tissues, including the fetal heart. Comprehensive timed-mating studies are consistent with our previously reported findings that Rlf homozygous mutant mice rarely survive to adulthood, with the majority dying shortly after birth...
December 5, 2016: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/27930729/strain-level-discrimination-of-shiga-toxin-producing-escherichia-coli-in-spinach-using-metagenomic-sequencing
#12
Susan R Leonard, Mark K Mammel, David W Lacher, Christopher A Elkins
Consumption of fresh bagged spinach contaminated with Shiga toxin-producing Escherichia coli (STEC) has led to severe illness and death; however current culture-based methods to detect foodborne STEC are time consuming. Since not all STEC strains are considered pathogenic to humans, it is crucial to incorporate virulence characterization of STEC in the detection method. In this study, we assess the comprehensiveness of utilizing a shotgun metagenomics approach for detection and strain-level identification by spiking spinach with a variety of genomically disparate STEC strains at a low contamination level of 0...
2016: PloS One
https://www.readbyqxmd.com/read/27930681/transcriptomic-changes-in-liver-of-young-bulls-caused-by-diets-low-in-mineral-and-protein-contents-and-supplemented-with-n-3-fatty-acids-and-conjugated-linoleic-acid
#13
Sara Pegolo, Alessio Cecchinato, Núria Mach, Massimiliano Babbucci, Marianna Pauletto, Luca Bargelloni, Stefano Schiavon, Giovanni Bittante
The aim of the present study was to identify transcriptional modifications and regulatory networks accounting for physiological and metabolic responses to specific nutrients in the liver of young Belgian Blue × Holstein bulls using RNA-sequencing. A larger trial has been carried out in which animals were fed with different diets: 1] a conventional diet; 2] a low-protein/low-mineral diet (low-impact diet) and 3] a diet enriched in n-3 fatty acids (FAs), conjugated linoleic acid (CLA) and vitamin E (nutraceutical diet)...
2016: PloS One
https://www.readbyqxmd.com/read/27930558/urinary-%C3%AE-trace-protein-a-unique-biomarker-to-screen-early-glomerular-filtration-rate-impairment
#14
Carlo Donadio, Laura Bozzoli
The screening for chronic kidney disease (CKD) patients needs the measurement of serum markers like creatinine. Our previous results indicated that urinary excretion of β-trace protein (BTP), a low-molecular-weight protein (23-29 kDa), is increased in CKD patients from stage 2. The aim of this study was to assess the major determinants of urinary excretion of BTP and to evaluate its feasibility as noninvasive marker of glomerular filtration rate (GFR) impairment.We studied 355 CKD patients (198 males), aged 15 to 83 years, in stable clinical conditions, classified in the different stages of CKD on the basis of GFR (renal clearance of Tc-diethylenetriamine penta-acetic acid)...
December 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27930359/the-role-of-whey-acidic-protein-four-disulfide-core-proteins-in-respiratory-health-and-disease
#15
Donna M Small, Declan F Doherty, Caoifa M Dougan, Sinéad Weldon, Clifford C Taggart
Members of the whey acidic protein (WAP) or WAP four-disulfide-core (WFDC) family of proteins are a relatively under-explored family of low molecular weight proteins. The two most prominent WFDC proteins, secretory leukocyte protease inhibitor (SLPI) and elafin (or the precursor, trappin-2), have been shown to possess multiple functions including anti-protease, anti-bacterial, anti-viral and anti-inflammatory properties. It is therefore of no surprise that both SLPI and elafin/trappin-2 have been developed as potential therapeutics...
December 8, 2016: Biological Chemistry
https://www.readbyqxmd.com/read/27929458/in-vivo-biosensor-tracks-non-apoptotic-caspase-activity-in-drosophila
#16
Ho Lam Tang, Ho Man Tang, Ming Chiu Fung, J Marie Hardwick
Caspases are the key mediators of apoptotic cell death via their proteolytic activity. When caspases are activated in cells to levels detectable by available technologies, apoptosis is generally assumed to occur shortly thereafter. Caspases can cleave many functional and structural components to cause rapid and complete cell destruction within a few minutes. However, accumulating evidence indicates that in normal healthy cells the same caspases have other functions, presumably at lower enzymatic levels. Studies of non-apoptotic caspase activity have been hampered by difficulties with detecting low levels of caspase activity and with tracking ultimate cell fate in vivo...
November 27, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27929144/intermolecular-interactions-in-the-tmem16a-dimer-controlling-channel-activity
#17
Paolo Scudieri, Ilaria Musante, Ambra Gianotti, Oscar Moran, Luis J V Galietta
TMEM16A and TMEM16B are plasma membrane proteins with Ca(2+)-dependent Cl(-) channel function. By replacing the carboxy-terminus of TMEM16A with the equivalent region of TMEM16B, we obtained channels with potentiation of channel activity. Progressive shortening of the chimeric region restricted the "activating domain" to a short sequence close to the last transmembrane domain and led to TMEM16A channels with high activity at very low intracellular Ca(2+) concentrations. To elucidate the molecular mechanism underlying this effect, we carried out experiments based on double chimeras, Forster resonance energy transfer, and intermolecular cross-linking...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27929140/acfs-accurate-circrna-identification-and-quantification-from-rna-seq-data
#18
Xintian You, Tim Of Conrad
Circular RNAs (circRNAs) are a group of single-stranded RNAs in closed circular form. They are splicing-generated, widely expressed in various tissues and have functional implications in development and diseases. To facilitate genome-wide characterization of circRNAs using RNA-Seq data, we present a freely available software package named acfs. Acfs allows de novo, accurate and fast identification and abundance quantification of circRNAs from single- and paired-ended RNA-Seq data. On simulated datasets, acfs achieved the highest F1 accuracy and lowest false discovery rate among current state-of-the-art tools...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27928969/new-candidate-genes-for-lack-of-sensitivity-to-therapy-in-pediatric-leukemias
#19
Weronika Bereza, Joanna Szczepanek, Joanna Laskowska, Andrzej Tretyn
In recent years, significant development of molecular genetics has contributed to the better understanding of leukemogenesis and classification of the different leukemia subtypes. Patients diagnosed with acute leukemia usually undergo chemotherapy, which involves the induction of remission, consolidation of remission and maintenance therapy. Patients are still vulnerable to relapse due to differences in the sensitivity of leukemia cells to the chemotherapeutic agent, because of the active drugs removal from the cells, improving the repair of DNA damage or abnormalities in the apoptotic pathway...
December 8, 2016: Current Cancer Drug Targets
https://www.readbyqxmd.com/read/27928940/epidemiological-and-pathological-investigation-of-fowl-aviadenovirus-serotypes-8b-and-11-isolated-from-chickens-with-inclusion-body-hepatitis-in-spain-2011-2013
#20
S Oliver-Ferrando, R Dolz, C Calderón, R Valle, R Rivas, M Pérez, M Biarnés, A Blanco, K Bertran, A Ramis, N Busquets, N Majó
Inclusion body hepatitis caused by different fowl aviadenovirus (FAdV) serotypes has been described in several countries in recent years. In Spain, from the spring of 2011 to 2013, an increased number of outbreaks in broiler and broiler breeder flocks from different regions occurred. The objectives of the present work were to carry out the molecular characterization of FAdV strains from Spanish inclusion body hepatitis cases and to study the pathogenicity and viral dynamics of these strains in specific pathogen-free (SPF) chickens...
December 8, 2016: Avian Pathology: Journal of the W.V.P.A
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