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https://www.readbyqxmd.com/read/28093775/how-does-temperature-influences-the-development-of-lactococcosis-transcriptomic-and-immunoproteomic-in%C3%A2-vitro-approaches
#1
R Castro, M Reguera-Brito, G H López-Campos, M M Blanco, M Aguado-Urda, J F Fernández-Garayzábal, A Gibello
Lactococcus garvieae is the aetiological agent of lactococcosis, a haemorrhagic septicaemia that affects marine and freshwater fish, with special incidence and economic relevance in farmed rainbow trout. Water temperature is one of the most important predisposing factors in the development of lactococcosis outbreaks. Lactococcosis in trout usually occur when water temperatures rise to about 18 °C, while fish carriers remain asymptomatic at temperatures below 13 °C. The aim of this work was to analyse the differences in the complete transcriptome response of L...
January 17, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28091657/zinc-transport-and-the-inhibition-of-the-l-type-calcium-channel-are-two-separable-functions-of-znt-1
#2
Eden Shusterman, Ofer Beharier, Shiri Levy, Raz Zarivach, Yoram Etzion, Craig R Campbell, Il-Ha Lee, Anuwat Dinudom, David I Cook, Asher Peretz, Amos Katz, Daniel Gitler, Arie Moran
Traditionally, proteins are considered to perform a single role, be it as an enzyme, a channel, a transporter or as a structural scaffold. However, recent studies have described moonlighting proteins that perform distinct and independent functions; for example, TRPM7 is both an ion channel and a kinase. ZnT-1 is a member of the Carrier Diffusion Facilitator family that is expressed throughout the phylogenetic tree from bacteria to humans. Since its cloning in 1995, ZnT-1 is considered a major extruder of Zn(2+) based on its capability to protect cells against zinc toxicity...
January 16, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28088526/subcutaneous-immunization-with-streptococcus-pneumoniae-gapdh-confers-effective-protection-in-mice-via-tlr2-and-tlr4
#3
Xiaoyu Sun, Jichao Wang, Jie Zhou, Hong Wang, Xiaofang Wang, Jingwen Wu, Yujuan He, Yibing Yin, Xuemei Zhang, Wenchun Xu
The surface localized Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Streptococcus pneumoniae is best known as housekeeping protein. Currently, GAPDH has been recognized as moonlighting protein and virulent factor. Therefore, we investigate whether GAPDH can act as a suitable vaccine candidate protein to prevent pneumococcal infection. In this study, mice received subcutaneous vaccination with recombinant GAPDH followed by challenge with D39 and 19F showing higher survival rate and lower bacterial loads in nasal washes and lung homogenates than control...
January 12, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28079255/moonlighting-at-replication-forks-a-new-life-for-homologous-recombination-proteins-brca1-brca2-and-rad51
#4
REVIEW
Arun Mouli Kolinjivadi, Vincenzo Sannino, Anna de Antoni, Hervé Técher, Giorgio Baldi, Vincenzo Costanzo
Coordination between DNA replication and DNA repair ensures maintenance of genome integrity, which is lost in cancer cells. Emerging evidence has linked Homologous Recombination (HR) proteins RAD51, BRCA1 and BRCA2 to the stability of nascent DNA. This function appears to be distinct from Double Strand Break (DSB) repair and is in part due to the prevention of MRE11-mediated degradation of nascent DNA at stalled forks. The role of RAD51 in fork protection resembles the activity described for its prokaryotic ortholog RecA, which prevents nuclease-mediated degradation of DNA and promotes replication fork restart in cells challenged by DNA damaging agents...
January 12, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28011643/deciphering-key-residues-involved-in-the-virulence-promoting-interactions-between-streptococcus-pneumoniae-and-human-plasminogen
#5
Christophe Moreau, Rémi Terrasse, Nicole M Thielens, Thierry Vernet, Christine Gaboriaud, Anne Marie Di Guilmi
Bacterial pathogens recruit circulating proteins to their own surfaces, coopting the host protein functions as a mechanism of virulence. Particular attention has focused on the binding of plasminogen (Plg) to bacterial surfaces, as it has been shown that this interaction contributes to bacterial adhesion to host cells, invasion of host tissues and evasion of the immune system. Several bacterial proteins are known to serve as receptors for Plg including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a cytoplasmic enzyme that appears on the cell surface in this moonlighting role...
December 23, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27989763/the-multifaceted-roles-of-leptospira-enolase
#6
Natália Salazar, Matilde Costa Lima de Souza, Amanda Gameiro Biasioli, Ludmila Bezerra da Silva, Angela Silva Barbosa
A previous study had demonstrated that Leptospira enolase is secreted extracellularly by a yet unknown mechanism and reassociates with the bacterial membrane. Surface-anchored leptospiral enolase displays plasminogen binding activity. In this work, we explored the consequences of this interaction and also assessed whether Leptospira enolase might display additional moonlighting functions by interacting with other host effector proteins. We first demonstrated that enolase-bound plasminogen is converted to its active form, plasmin...
October 28, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27983623/multiple-forms-of-glutamate-dehydrogenase-in-animals-structural-determinants-and-physiological-implications
#7
REVIEW
Victoria Bunik, Artem Artiukhov, Vasily Aleshin, Garik Mkrtchyan
Glutamate dehydrogenase (GDH) of animal cells is usually considered to be a mitochondrial enzyme. However, this enzyme has recently been reported to be also present in nucleus, endoplasmic reticulum and lysosomes. These extramitochondrial localizations are associated with moonlighting functions of GDH, which include acting as a serine protease or an ATP-dependent tubulin-binding protein. Here, we review the published data on kinetics and localization of multiple forms of animal GDH taking into account the splice variants, post-translational modifications and GDH isoenzymes, found in humans and apes...
December 14, 2016: Biology
https://www.readbyqxmd.com/read/27983481/the-intestinal-proteome-of-diabetic-and-control-children-is-enriched-with-different-microbial-and-host-proteins
#8
Elsa Pinto, Marisol Anselmo, Manuela Calha, Andrew Bottrill, Isabel Duarte, Peter Andrew, Maria Leonor Faleiro
In this study the intestinal microbial proteome of children with established type 1 diabetes (T1D) was compared with the proteome of healthy children (Control) with the aim to identify differences in the activity of the intestinal microbiota that will not only contribute to a deeper knowledge of the functionality of the gut in these children but may also provide new approaches to improve the Control of the disease. Fecal protein extracts collected from three T1D children (aged of 9.3 ± 0.6) and three Control children (aged of 9...
December 15, 2016: Microbiology
https://www.readbyqxmd.com/read/27983472/characterization-of-two-new-putative-adhesins-of-leptospira-interrogans
#9
Jupciana Figueredo, Gabriela Siqueira, Gisele Souza, Marcos Heinemann, Silvio Vasconcellos, Erica Chapola, Ana Lucia T O Nascimento
We here report the characterization of two novel proteins encoded by the genes LIC11122 and LIC12287, identified in the genome sequences of Leptospira interrogans, annotated, respectively, as a putative sigma factor and hypothetical. . The coding sequences (CDSs) LIC11122 and LIC12287 have signal peptide SPII and SPI and are predicted to be located mainly at the cytoplasm membrane of the bacteria. The genes were cloned and the proteins expressed using Escherichia coli. Proteinase K digestion showed that both proteins are surface exposed...
December 15, 2016: Microbiology
https://www.readbyqxmd.com/read/27973707/moonlighting-chaperone-like-activity-of-the-universal-regulatory-14-3-3-proteins
#10
REVIEW
Nikolai N Sluchanko, Nikolai B Gusev
The ubiquitous eukaryotic 14-3-3 proteins coordinate multiple cellular processes due to their well-known regulatory function that is based on specific recognition of phosphorylated motifs in their partners. In this context, 14-3-3 proteins have, in reports, been called 'chaperones'. Although in the classical meaning this is not fully correct, recent studies have revealed that 14-3-3 can indeed be an integral part of the protein quality control system, as they: (i) display ATP-independent anti-aggregation ('holdase') activity, similar to that of the unrelated small heat shock proteins; (ii) assist in clearing misfolded proteins by directing them to proteasomes or aggresomes; (iii) cooperate with classical chaperones for substrate refolding; and also (iv) are associated with neurodegenerative disorders by affecting aggregation of tau, prion protein, α-synuclein, and huntingtin, etc...
December 14, 2016: FEBS Journal
https://www.readbyqxmd.com/read/27932461/structural-characterization-and-ligand-inhibitor-identification-provide-functional-insights-into-the-mycobacterium-tuberculosis-cytochrome-p450-cyp126a1
#11
Jude T Chenge, Duyet Van Le, Shalini Swami, Kirsty J McLean, Madeline E Kavanagh, Anthony G Coyne, Stephen E J Rigby, Myles R Cheesman, Hazel M Girvan, Colin W Levy, Bernd Rupp, Jens P von Kries, Chris Abell, David Leys, Andrew W Munro
The Mycobacterium tuberculosis (Mtb) H37Rv genome encodes 20 cytochromes P450, including P450s crucial to infection and bacterial viability. Many Mtb P450s remain uncharacterized, suggesting their further analysis may provide new insights into Mtb metabolic processes and new targets for drug discovery. CYP126A1 is representative of a P450 family widely distributed in mycobacteria and other bacteria. Here we explore the biochemical and structural properties of CYP126A1, including its interactions with new chemical ligands...
December 8, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27912006/cascading-effects-of-predation-risk-determine-how-marine-predators-become-terrestrial-prey-on-an-oceanic-island
#12
Sarah K Thomsen, David J Green
Apex predators can suppress the foraging activity of mesopredators, which may then result in cascading benefits for the prey of those mesopredators. We studied the interactions between a top predator, the Barn Owl (Tyto alba), and their primary prey, an island endemic deer mouse (Peromyscus maniculatus elusus), which in turn consumes the eggs of seabirds nesting on Santa Barbara Island in California. Scripps's Murrelets (Synthliboramphus scrippsi), a threatened nocturnal seabird, arrive annually to breed on this island, and whose first egg is particularly vulnerable to predation by mice...
December 2016: Ecology
https://www.readbyqxmd.com/read/27899560/not5-dependent-co-translational-assembly-of-ada2-and-spt20-is-essential-for-functional-integrity-of-saga
#13
Sari Kassem, Zoltan Villanyi, Martine A Collart
Acetylation of histones regulates gene expression in eukaryotes. In the yeast Saccharomyces cerevisiae it depends mainly upon the ADA and SAGA histone acetyltransferase complexes for which Gcn5 is the catalytic subunit. Previous screens have determined that global acetylation is reduced in cells lacking subunits of the Ccr4-Not complex, a global regulator of eukaryotic gene expression. In this study we have characterized the functional connection between the Ccr4-Not complex and SAGA. We show that SAGA mRNAs encoding a core set of SAGA subunits are tethered together for co-translational assembly of the encoded proteins...
November 28, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27885629/glutaminases
#14
Javier Márquez, José M Matés, José A Campos-Sandoval
Mammalian glutaminases catalyze the stoichiometric conversion of L-glutamine to L-glutamate and ammonium ions. In brain, glutaminase is considered the prevailing pathway for synthesis of the neurotransmitter pool of glutamate. Besides neurotransmission, the products of glutaminase reaction also fulfill crucial roles in energy and metabolic homeostasis in mammalian brain. In the last years, new functional roles for brain glutaminases are being uncovered by using functional genomic and proteomic approaches. Glutaminases may act as multifunctional proteins able to perform different tasks: the discovery of multiple transcript variants in neurons and glial cells, novel extramitochondrial localizations, and isoform-specific proteininteracting partners strongly support possible moonlighting functions for these proteins...
2016: Advances in Neurobiology
https://www.readbyqxmd.com/read/27882614/%C3%AE-ketoglutarate-dehydrogenase-complex-moonlighting-ros-signalling-added-to-the-list-an-editorial-highlight-for-reductions-in-the-mitochondrial-enzyme-%C3%AE-ketoglutarate-dehydrogenase-complex-in-neurodegenerative-disease-beneficial-or-detrimental
#15
EDITORIAL
Emilio Fernandez, Juan P Bolaños
Read the highlighted article 'Reductions in the mitochondrial enzyme α-ketoglutarate dehydrogenase complex in neurodegenerative disease - beneficial or detrimental?' on page 823.
December 2016: Journal of Neurochemistry
https://www.readbyqxmd.com/read/27867805/isolation-of-lactic-acid-bacteria-bound-to-the-porcine-intestinal-mucosa-and-an-analysis-of-their-moonlighting-adhesins
#16
Hideki Kinoshita, Satoko Ohuchi, Kensuke Arakawa, Masamichi Watanabe, Haruki Kitazawa, Tadao Saito
The adhesion of lactic acid bacteria (LAB) to the intestinal mucosa is one of the criteria in selecting for probiotics. Eighteen LAB were isolated from porcine intestinal mucin (PIM): ten strains of Lactobacillus, six strains of Weissella, and two strains of Streptococcus. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for phosphate-buffered saline (PBS) extracts from the LAB, many bands were detected in half of the samples, while a few and/or no clear bands were detected in the other half...
2016: Bioscience of Microbiota, Food and Health
https://www.readbyqxmd.com/read/27867042/poly-adp-ribose-polymerase-1-hyperactivation-in-neurodegenerative-diseases-the-death-knell-tolls-for-neurons
#17
REVIEW
Parimala Narne, Vimal Pandey, Praveen Kumar Simhadri, Prakash Babu Phanithi
Neurodegeneration is a salient feature of chronic refractory brain disorders like Alzheimer's, Parkinson's, Huntington's, amyotropic lateral sclerosis and acute conditions like cerebral ischemia/reperfusion etc. The pathological protein aggregates, mitochondrial mutations or ischemic insults typifying these disease conditions collude with and intensify existing oxidative stress and attendant mitochondrial dysfunction. Interlocking these mechanisms is poly(ADP-ribose) polymerase (PARP-1) hyperactivation that invokes a distinct form of neuronal cell death viz...
November 17, 2016: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/27865402/some-proteins-of-m-%C3%A2-tuberculosis-that-localise-to-the-nucleus-of-thp-1-derived-macrophages
#18
Atul Kumar Agrawal, Rajeev Ranjan, Sharat Chandra, Tofan Kumar Rout, Amit Misra, Thota Jagadeshwar Reddy
Host-pathogen dialectics in tuberculosis (TB) via DNA-protein interactions are emerging. We investigated whether proteins produced by Mycobacterium tuberculosis (Mtb) could translocate to the host nucleus. Using lysates of nuclei purified from Mtb-infected THP-1-derived macrophages, we identified at least 15 proteins of Mtb-origin by electrophoretic and chromatographic separation and mass spectrometry. Western blotting confirmed time-dependent accumulation of Mtb EF-Tu, GroEL, GroES and MtrA in the host nucleus...
December 2016: Tuberculosis
https://www.readbyqxmd.com/read/27853572/anthropogenic-disruption-of-the-night-sky-darkness-in-urban-and-rural-areas
#19
Salvador Bará
The growing emissions of artificial light to the atmosphere are producing, among other effects, a significant increase of the night sky brightness (NSB) above its expected natural values. A permanent sensor network has been deployed in Galicia (northwest of Iberian peninsula) to monitor the anthropogenic disruption of the night sky darkness in a countrywide area. The network is composed of 14 detectors integrated in automated weather stations of MeteoGalicia, the Galician public meteorological agency. Zenithal NSB readings are taken every minute and the results are openly available in real time for researchers, interested stakeholders and the public at large through a dedicated website...
October 2016: Royal Society Open Science
https://www.readbyqxmd.com/read/27833947/erythropoietin-and-co-intrinsic-structure-and-functional-disorder
#20
REVIEW
Vladimir N Uversky, Elrashdy M Redwan
Erythropoietin (Epo) is a heavily glycosylated protein, with its main function being related to erythropoiesis, where it controls red blood cell production via interaction with the Epo receptor (EpoR). It also plays a number of important roles in various hormonal, growth factor, and cytokine pathways. These roles are defined by Epo partners, such as the homodimeric (EpoR)2 receptor, the heterodimeric EpoR/βCR receptor and hypoxia inducing factor (HIF). Although the main structural features of both Epo and EpoR are conserved in vertebrates, the secretion sites of Epo in mammals are different from those in other vertebrates...
December 20, 2016: Molecular BioSystems
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