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chitin wound healing

Huixin Jiang, Takaomi Kobayashi
Ultrasound (US) stimulated drug release was examined in this study using a chitin hydrogel matrix loaded with gallic acid (GA), a drug used for wound healing and anticancer. Using phase inversion, GA-chitin hydrogels were prepared from chitin-dimethylacetamide (DMAc)/lithium chloride (LiCl) solution in the presence of GA, with 24h exposure of the solution to water vapor. The GA release from the GA-chitin hydrogel was examined under different US powers of 0-30W at 43kHz. The effects of GA loading amounts in the hydrogels (0...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Faez Iqbal Khan, Safikur Rahman, Aarfa Queen, Shahzaib Ahamad, Sher Ali, Jihoe Kim, Md Imtaiyaz Hassan
Chitin is a long unbranched polysaccharide, made up of β-1,4-linked N-acetylglucosamine which forms crystalline fiber-like structure. It is present in the fungal cell walls, insect and crustacean cuticles, nematode eggshells, and protozoa cyst. We provide a critical appraisal on the chemical modifications of chitin and its derivatives in the context of their improved efficacy in medical applications without any side effect. Recent advancement in nanobiotechnology has helped to synthesize several chitin derivatives having significant biological applications...
May 2017: Applied Microbiology and Biotechnology
Thiago B Cahú, Rodrigo A Silva, Raquel P F Silva, Milena M Silva, Isabel R S Arruda, Janilson F Silva, Romero M P B Costa, Suzan D Santos, Helena B Nader, Ranilson S Bezerra
In this work, chitosan-based films containing gelatin and chondroitin-4-sulfate (C4S) with and without ZnO particles were produced and tested in vitro to investigate their potential wound healing properties. Chitosans were produced from shrimp-head processing waste by alkaline deacetylation of chitin to obtain chitosans differing in molecular weight and degree of deacetylation (80 ± 0.5%). The film-forming solutions (chitosan, C4S and gelatin) and ZnO suspension showed no toxicity towards fibroblasts or keratinocytes...
March 27, 2017: Applied Biochemistry and Biotechnology
Jun Kinoda, Masayuki Ishihara, Hidemi Hattori, Shingo Nakamura, Koichi Fukuda, Hidetaka Yokoe
Size-controlled spherical silver nanoparticles (<10 nm) and chitin-nanofiber sheet composites (Ag NPs/CNFS) have previously been reported to have strong antimicrobial activity in vitro. Although Ag NPs/CNFS have strong antimicrobial activity, their cytotoxicity has not been investigated. This study was performed to evaluate the effects of Ag NPs/CNFS on cytotoxicity for fibroblasts in vitro and healing delay of wound repair in vivo, focused on oxidative stress. Cytotoxic activities of Ag NPs/CNFS were investigated using a fibroblast cell proliferation assay, nitric oxide/nitrogen dioxide (NO/NO₂) measurement of the cell lysates in vitro, inhibitory effects of Ag NPs/CNFS on healing-impaired wound repair using diabetic mice in vivo, 8-hydroxy-2'-deoxyguanosine (8-OHdG) immunohistochemical staining of the skin sections, and generation of carbonyl protein in the wound was performed to evaluate cytotoxicity with oxidative stress...
October 20, 2016: Nanomaterials
Jacek Drobnik, Izabella Krucinska, Agnieszka Komisarczyk, Stanislaw Sporny, Alicja Szczepanowska, Joanna Ciosek
BACKGROUND: We sought to determine the usefulness of electrospun dibutyrylchitin (DBC) or poly-(ε-caprolactone [PCL]), in wound treatment. We investigated the mechanisms of action of these polymers on wound healing. METHODS: We synthesized DBC, a newly identified ester derivative of chitin, using a patented method comprising the substitution of butyryl groups at positions C-3 and C-6 in chitin molecules. We confirmed the double substitution by the butyric groups using infrared spectrometry...
June 2017: Canadian Journal of Surgery. Journal Canadien de Chirurgie
Kangquan Shou, Yao Huang, Baiwen Qi, Xiang Hu, Zhanjun Ma, Ang Lu, Chao Jian, Lina Zhang, Aixi Yu
Transplantation of bone marrow mesenchymal stem cells (BMSCs) has been considered to be a promising strategy for wound healing. However, poor viability of engrafted BMSCs and limited capabilities of differentiation into the desired cell types in wounds often hinder its application. Few studies report the induction of BMSC differentiation into the skin regeneration-related cell types using natural biopolymer, e.g. chitin and its derivative. Here we utilized a chitin nanofiber (CNF) hydrogel as a directive cue to induce BMSC differentiation for enhancing cutaneous wound regeneration in the absence of cell-differentiating factors...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
Narguess H Marei, W El-Mazny, Aida El-Shaer, Kareem Dorri Zaki, Zahra S Hussein, Emtithal M Abd-El-Samie
Chitosan (CS) has received great attention in tissue engineering, especially in wound healing acceleration. In this study, chitin was isolated from desert locust (Schistocerca gregaria) and shrimp (Penaeus monodon) then deacetylated to chitosan. Then, chitosan was characterized by degree of deacetylation (DD), molecular weight (M.Wt), swelling index (SI), Fourier transform infrared (FTIR) and X ray diffraction (XRD). The chitosan was then casted into 2D scaffolds and was pictured using scanning electron microscope (SEM)...
April 2017: International Journal of Biological Macromolecules
Kai Zhao, Dan Li, Hong Kang, Yu Guang Rong, Xiaohua Wang, Zheng Jin
Chitosan, a natural polysaccharide derived from chitin, has been widely applied in drug delivery, tissue regeneration, wound healing, blood coagulation and immunostimulation due to its well-known biocompatibility and biodegradability. Chitosan has become a valuable vaccine adjuvant and delivery carrier, which have attracted increasing attention for its applications. Currently chitosan nanoparticles have been developed as adjuvant or immunomodulators and drug carrier. Here we reviewed chitosan nanoparticles, which is a promising biomaterial as vaccine adjuvant and delivery carrier, including characteristics, preparation methods and applications, or even its limitations...
August 4, 2016: Current Drug Delivery
Dan Li, Dongwei Fu, Hong Kang, Guangyu Rong, Zheng Jin, Xiaohua Wang, Kai Zhao
BACKGROUND: Drug research and development has entered into the new epoch of innovation formulation, and the drug delivery system has been in the forefront of pharmaceutical innovation. Chitosan, a natural polysaccharide derived from chitin, due to its well-known biocompatibility and biodegradability, it has been widely used in drug delivery, immunostimulation, tissue regeneration, blood coagulation, wound healing, drug delivery and tissue engineering. Chitosan has become a valuable vaccine adjuvant and delivery carrier, which have attracted increasing attention for its applications...
2017: Current Drug Delivery
Marius Rădulescu, Alina Maria Holban, Laurențiu Mogoantă, Tudor-Adrian Bălşeanu, George Dan Mogoșanu, Diana Savu, Roxana Cristina Popescu, Oana Fufă, Alexandru Mihai Grumezescu, Eugenia Bezirtzoglou, Veronica Lazar, Mariana Carmen Chifiriuc
The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic. Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques...
June 10, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
A M Abdel-Mohsen, J Jancar, D Massoud, Z Fohlerova, H Elhadidy, Z Spotz, A Hebeish
Chitin/chitosan-glucan complex (ChCsGC) was isolated from Schizophyllum commune (S. commune) and dissolved for the first time in precooled (-15°C) 8wt.% urea/6wt.% NaOH aqueous solution. Novel nonwoven microfiber mats were fabricated by wet-dry-spinning technique and evaluated the mechanical of fabrics mats and surface morphology. Isolated and nonwoven mat were characterized employing FTIR-ATR, Optical microscope, TGA, DSC, H/C NMR, SEM and XRD techniques. According to the physical/chemical characterization measurements we can assumed that, the net and the novel dressing mats have the same chemical structure with slightly changes in the thermal stability for the dressing mats...
August 20, 2016: International Journal of Pharmaceutics
Roman S Esipov, Dmitry A Makarov, Vasily N Stepanenko, Anatoly I Miroshnikov
Thymosin β4 is a 43 amino acid long peptide with an acetylated N-terminal serin that has a high potential as a remedy for healing ulcers, wounds and burns. Although protein biosynthesis offers attractive opportunities in terms of a large-scale production, currently thymosin β4 is mainly produced by chemical synthesis. The problems that hinder the successful commercialization of the biotechnological approach are associated with the small peptides expression and N-terminal acetylation. This work presents an innovative biotechnological method for thymosin β4 production that employs the peptide acetylation in vivo...
June 20, 2016: Journal of Biotechnology
Wei Zhang, Yan Wang, Xianxian Sui, Yulin Sun, Daohai Chen
PURPOSE: To detect whether chitin and sepia ink sponge (CS) can promote wound healing and elevate impact of CS on phagocytosis ability of macrophages. METHODS: Forty-eight rats were assigned to four groups: Normal group (Normal), negative control group (Con), chitin and sepia ink sponge group (CS) and positive control Surgicel Gauze(r) group (SG). Deep second-degree burn model was created in rats. Wound area was recorded by digital imaging and determined using Image J software...
February 2016: Acta Cirúrgica Brasileira
Ammara Rafique, Khalid Mahmood Zia, Mohammad Zuber, Shazia Tabasum, Saima Rehman
Chitin and chitosan are amino polysaccharides having multidimensional properties, such as biocompatibility, biodegradability, antibacterial properties and non-toxicity, muco-adhesivity, adsorption properties, etc., and thus they can be widely used in variety of areas. Although human history mainly relies on the biopolymers, however synthetic materials like polyvinyl alcohol (PVA) have good mechanical, chemical and physical properties. Functionalization of PVA with chitin and chitosan is considered very appropriate for the development of well-designed biomaterials such as biodegradable films, for membrane separation, for tissue engineering, for food packaging, for wound healing and dressing, hydro gels formation, gels formation, etc...
June 2016: International Journal of Biological Macromolecules
Yu Zhu, Xin-Hai Ye, Yang Liu, Zhi-Chao Yan, David Stanley, Gong-Yin Ye, Qi Fang
Chitin-binding proteins (CBPs) are present in many species and they act in a variety of biological processes. We analyzed a Pteromalus puparum venom apparatus proteome and transcriptome and identified a partial gene encoding a possible CBP. Here, we report cloning a full-length cDNA of a sequence encoding a chitin-binding-like protein (PpCBP) from P. puparum, a pupal endoparasitoid of Pieris rapae. The cDNA encoded a 96-amino-acid protein, including a secretory signal peptide and a chitin-binding peritrophin-A domain...
December 2015: Toxins
Shoa Naqvi, Bruno M Moerschbacher
Chitin is one of the most abundant renewable resources, and chitosans, the partially deacetylated derivatives of chitin, are among the most promising functional biopolymers, with superior material properties and versatile biological functionalities. Elucidating molecular structure-function relationships and cellular modes of action of chitosans, however, it is challenging due to the micro-heterogeneity and structural complexity of polysaccharides. Lately, it has become apparent that many of the biological activities of chitosan polymers, such as in agricultural plant disease protection or in mediating scar-free wound healing, may be attributed to oligomeric break-down products generated by the action of chitosanolytic hydrolases present in the target tissues, such as human chitotriosidase...
February 2017: Critical Reviews in Biotechnology
Katrina J Binger, Matthias Gebhardt, Matthias Heinig, Carola Rintisch, Agnes Schroeder, Wolfgang Neuhofer, Karl Hilgers, Arndt Manzel, Christian Schwartz, Markus Kleinewietfeld, Jakob Voelkl, Valentin Schatz, Ralf A Linker, Florian Lang, David Voehringer, Mark D Wright, Norbert Hubner, Ralf Dechend, Jonathan Jantsch, Jens Titze, Dominik N Müller
A high intake of dietary salt (NaCl) has been implicated in the development of hypertension, chronic inflammation, and autoimmune diseases. We have recently shown that salt has a proinflammatory effect and boosts the activation of Th17 cells and the activation of classical, LPS-induced macrophages (M1). Here, we examined how the activation of alternative (M2) macrophages is affected by salt. In stark contrast to Th17 cells and M1 macrophages, high salt blunted the alternative activation of BM-derived mouse macrophages stimulated with IL-4 and IL-13, M(IL-4+IL-13) macrophages...
November 2, 2015: Journal of Clinical Investigation
Sung Cil Lim, Ki-Man Lee, Tae Jin Kang
Our previous report showed that the extract from cuttlebone (CB) had wound healing effect in burned lesion of rat and the extract was identified as chitin by HPLS analysis. We herein investigated the morphology in CB extract using scanning electron microscope (SEM). Chitin was used as a control. There is no difference in morphology between CB extract and chitin. We also assessed the role of CB extract on the production of inflammatory mediators using murine macrophages and the migration of inflammatory cells...
July 2015: Biomolecules & Therapeutics
Kazuo Azuma, Shinsuke Ifuku, Tomohiro Osaki, Yoshiharu Okamoto, Saburo Minami
Chitin (β-(1-4)-poly-N-acetyl-D-glucosamine) is widely distributed in nature and is the second most abundant polysaccharide after cellulose. Chitin occurs in nature as ordered macrofibrils. It is the major structural component in the exoskeleton of crab and shrimp shells and the cell wall of fungi and yeast. As chitin is not readily dissolved in common solvents, it is often converted to its more deacetylated derivative, chitosan. Chitin, chitosan, and its derivatives are widely used in tissue engineering, wound healing, and as functional foods...
October 2014: Journal of Biomedical Nanotechnology
Xiaohui Mo, John Cen, Elaine Gibson, Robin Wang, Steven L Percival
Chitosan, a natural polysaccharide derivate from chitin, offers a promising alternative biomaterial for use in wound dressings. In this work, the safety and efficacy of a next-generation KA01 chitosan wound dressing in facilitating the healing of nonhealing chronic wounds was studied. This open multicenter comparative prospective randomized clinical study was conducted at three medical centers in China. A total of 90 patients (45 in test group and 45 in control group) with unhealed chronic wounds including pressure ulcers, vascular ulcers, diabetic foot ulcers, and wounds with minor infections, or at risk of infection, were treated with the next generation chitosan wound dressing as the test article or traditional vaseline gauze as a control...
July 2015: Wound Repair and Regeneration
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