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https://www.readbyqxmd.com/read/28748627/genetic-ablation-of-carbonic-anhydrase-ix-disrupts-gastric-barrier-function-via-claudin-18-downregulation-and-acid-backflux
#1
Taolang Li, Xuemei Liu, Brigitte Riederer, Katerina Nikolovska, Anurag Kumar Singh, Kari A Mäkelä, Anna Seidler, Yongjian Liu, Gerolf Gros, Helmut Bartels, Karl Heinz Herzig, Ursula Seidler
AIM: The study aims to explore the molecular mechanisms for the parietal cell loss and fundic hyperplasia observed in gastric mucosa of mice lacking the carbonic anhydrase 9 (CAIX). METHODS: We assessed the ability of CAIX knockout and WT gastric surface epithelial cells to withstand luminal acid load by measuring the pHi of exteriorized gastric mucosa in vivousing two photon confocal laser scanning microscopy. Cytokines and claudin-18A2 expression was analyzed by RT-PCR...
July 27, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28746419/glass-import-and-production-in-hispania-during-the-early-medieval-period-the-glass-from-ciudad-de-vascos-toledo
#2
Jorge de Juan Ares, Nadine Schibille
One hundred and forty-one glass fragments from medieval Ciudad de Vascos (Toledo, Spain) were analysed by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The glasses fall into three types according to the fluxing agents used: mineral natron, soda-rich plant ash, and a combination of soda ash and lead. The natron glasses can be assigned to various established primary production groups of eastern Mediterranean provenance. Different types of plant ash glasses indicate differences in the silica source as well as the plant ash component, reflecting changing supply mechanisms...
2017: PloS One
https://www.readbyqxmd.com/read/28746367/high-resolution-isotopic-evidence-of-specialised-cattle-herding-in-the-european-neolithic
#3
Claudia Gerling, Thomas Doppler, Volker Heyd, Corina Knipper, Thomas Kuhn, Moritz F Lehmann, Alistair W G Pike, Jörg Schibler
Reconstructing stock herding strategies and land use is key to comprehending past human social organization and economy. We present laser-ablation strontium and carbon isotope data from 25 cattle (Bos taurus) to reconstruct mobility and infer herding management at the Swiss lakeside settlement of Arbon Bleiche 3, occupied for only 15 years (3384-3370 BC). Our results reveal three distinct isotopic patterns that likely reflect different herding strategies: 1) localized cattle herding, 2) seasonal movement, and 3) herding away from the site year-round...
2017: PloS One
https://www.readbyqxmd.com/read/28746258/treatment-of-facial-acne-scarring-with-fractional-carbon-dioxide-laser-in-asians-a-retrospective-analysis-of-efficacy-and-complications
#4
Harumi Ochi, Lixian Tan, Wee Ping Tan, Chee Leok Goh
BACKGROUND: Fractional carbon dioxide (CO2) laser resurfacing unites the idea of fractional photothermolysis with an ablative 10,600-nm wavelength. This technology permits effective treatment of acne scarring, with shorter recovery and a decreased side effect profile as compared to traditional CO2 laser resurfacing. OBJECTIVE: The aims of this study were to study the efficacy and safety of fractional CO2 laser for acne scars in Asian patients. METHODS: This was a retrospective study of all patients treated with the fractional CO2 laser for facial acne scarring between January 2, 2008, and December 31, 2010, at the National Skin Centre, Singapore...
July 25, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28745654/-analysis-of-corneal-biomechanical-changes-after-femtosecond-laser-assisted-laser-in-situ-keratomileusis-in-children-with-hyperopic-anisometropia
#5
I L Kulikova, O V Shlenskaya, N V Chapurin
AIM: to analyze corneal hysteresis (CH) and corneal resistance factor (RF) readings obtained with the Reichert Ocular Response Analyzer (ORA) before and after hyperopic femtolaser-assisted laser in situ keratomileusis (FS-LASIK) in children with anisometropia as well as to establish factors that may cause changes in these parameters. MATERIAL AND METHODS: CH and RF were evaluated before and 1.5 years after FS-LASIK performed on amblyopic eyes of 28 patients aged 6-14 years...
2017: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/28745365/elemental-bio-imaging-of-pegylated-nayf4-yb-tm-gd-upconversion-nanoparticles-in-mice-by-laser-ablation-inductively-coupled-plasma-mass-spectrometry-to-study-toxic-side-effects-on-the-spleen-liver-and-kidneys
#6
Qing Li, Zheng Wang, Yirui Chen, Guoxia Zhang
Rare-earth upconversion nanoparticles (UCNPs) are considered stable nanoprobes with low toxicity and deep tissue penetration. However, the increasing use of UCNPs has raised concerns about their potential toxicity in living organisms. Very few studies have reported the toxicity of UCNPs; hence, it is not possible to conclude yet that UCNPs are safe. In this study, the distribution of PEGylated NaYF4:Yb/Tm/Gd nanoparticles (PEG-UCNPs) in female Balb/c mice following intravenous administration, and imaging in the spleen, liver and kidney was examined by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)...
July 26, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28742618/changes-in-higher-order-aberrations-after-phototherapeutic-keratectomy-for-subepithelial-corneal-infiltrates-after-epidemic-keratoconjunctivitis
#7
Burcin Kepez Yildiz, Semih Urvasizoglu, Yusuf Yildirim, Alper Agca, Nilay K Besek, Korhan Fazil, Erdem Eris, Ebru Demet Aygit, Muhittin Taskapili, Ahmet Demirok
PURPOSE: To investigate how corneal aberrations change after phototherapeutic keratectomy (PTK) for subepithelial infiltrates after adenoviral keratoconjunctivitis. METHODS: The records of patients who underwent transepithelial PTK for subepithelial infiltrates were retrospectively reviewed. Preoperative best-corrected visual acuity (VA) and the results of slit-lamp biomicroscopy examinations were recorded. The PTK procedure was performed under topical anesthesia with an Amaris excimer laser...
July 24, 2017: Cornea
https://www.readbyqxmd.com/read/28741634/mitochondria-targeting-near-infrared-light-triggered-thermosensitive-liposomes-for-localized-photothermal-and-photodynamic-ablation-of-tumors-combined-with-chemotherapy
#8
Caixia Yue, Yuming Yang, Jie Song, Gabriel Alfranca, Chunlei Zhang, Qian Zhang, Ting Yin, Fei Pan, Jesús M de la Fuente, Daxiang Cui
Lonidamine, an anticancer drug that acts on mitochondria, has poor water solubility. Mitochondria are the primary source of cellular reactive oxygen species (ROS), which are necessary for photodynamic therapy. Hence, a mitochondria-targeting drug delivery system loaded with Lonidamine and a ROS-produced photosensitizer could improve the bioavailability of Lonidamine and maximize photodynamic therapeutic efficiency. Here we report, for the first time, new IR-780 and Lonidamine encapsulated mitochondria-targeting thermosensitive liposomes (IL-TTSL)...
July 25, 2017: Nanoscale
https://www.readbyqxmd.com/read/28739488/nanosilver-pmma-composite-coating-optimized-to-provide-robust-antibacterial-efficacy-while-minimizing-human-bone-marrow-stromal-cell-toxicity
#9
Peter E Petrochenko, Jiwen Zheng, Brendan J Casey, Reza Bayati, Roger J Narayan, Peter L Goering
Porous PMMA is a versatile biomaterial with good biocompatibility but high susceptibility to bacterial colonization, which we mitigated by utilizing immobilized antimicrobial silver nanoparticles (AgNPs). A uniform porous thin film was deposited onto silicon wafers by simultaneously ablating PMMA and silver (Ag) using pulsed laser deposition (PLD) optimized for minimal human cell toxicity and antibacterial efficacy. PMMA without Ag became heavily colonized by E. coli in simulated dynamic conditions, while Ag-containing samples prevented all colonization...
July 21, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28738647/development-of-an-analytical-methodology-for-obtaining-quantitative-mass-concentrations-from-laap-tof-ms-measurements
#10
Rachel Gemayel, Brice Temime-Roussel, Nathalie Hayeck, Adrien Gandolfo, Stig Hellebust, Sasho Gligorovski, Henri Wortham
Laser ablation aerosol particle-time of flight mass spectrometer (LAAP-ToF-MS) measures the size number of particles, and chemical composition of individual particles in real-time. LAAP-ToF-MS measurements of chemical composition are difficult to quantify, mostly because the instrument sensitivities to various chemical species in the multicomponent atmospheric aerosol particles are unknown. In this study, we investigate a field-based approach for quantitative measurements of ammonium, nitrate, sulfate, OC, and EC, in size-segregated atmospheric aerosols, by LAAP-ToF-MS using concurrent measurements from high-resolution time-of-flight aerosol mass spectrometer (HR-ToF-AMS), and multi-angle absorption photometer (MAAP)...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28736902/pseudoamniotic-band-syndrome-after-fetoscopic-laser-ablation-of-placental-anastomoses-for-twin-twin-transfusion-syndrome-two-case-reports-and-systematic-review
#11
Anne Sophie Lafitte, Eric Verspyck, Corinne Jeanne Pasquier, Patricia Dolley, Michel Dreyfus, Guillaume Benoist
Pseudoamniotic band syndrome is a rare complication that occurs after invasive procedures for complicated monochorionic twins. We report 2 cases of intrauterine recipient fetal death after laser therapy for twin-twin transfusion syndrome due to umbilical cord constriction by the amniotic band.
July 24, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28736167/an-actomyosin-arf-gef-negative-feedback-loop-for-tissue-elongation-under-stress
#12
Junior J West, Teresa Zulueta-Coarasa, Janna A Maier, Donghoon M Lee, Ashley E E Bruce, Rodrigo Fernandez-Gonzalez, Tony J C Harris
In response to a pulling force, a material can elongate, hold fast, or fracture. During animal development, multi-cellular contraction of one region often stretches neighboring tissue. Such local contraction occurs by induced actomyosin activity, but molecular mechanisms are unknown for regulating the physical properties of connected tissue for elongation under stress. We show that cytohesins, and their Arf small G protein guanine nucleotide exchange activity, are required for tissues to elongate under stress during both Drosophila dorsal closure (DC) and zebrafish epiboly...
July 13, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28735079/synthesis-and-characterization-of-nanoparticles-conjugated-tannase-and-using-it-for-enhancement-of-antibacterial-activity-of-tannase-produced-by-serratia-marcescens
#13
D Sahira Nsayef Muslim, Ziyad Abbas Dham, D Nadheer J Mohammed
Fourteen isolates of Serratia marcescens were collected from patients suffering from septicemia. All theseisolates revealed different levels in tannase production. Tannase was partially purified from Serratia marcescens b9 by precipitation method at 70% saturation of ammonium sulfate. Au, Pt, SnO2 and SiO2 nanoparticles were prepared by laser ablation and examined by transmission electron microscopy (TEM), X-ray diffraction pattern and UV-Visible absorption spectroscopy. Conjugation of SiO2 nanoparticles to tannase by feeding and pulses methods were prepared and characterized by TEM, X-ray diffraction pattern and UV-Visible spectrum...
July 19, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28735009/serotonergic-network-in-the-subesophageal-zone-modulates-the-motor-pattern-for-food-intake-in-drosophila
#14
Andreas Schoofs, Sebastian Hückesfeld, Michael J Pankratz
The functional organization of central motor circuits underlying feeding behaviors is not well understood. We have combined electrophysiological and genetic approaches to investigate the regulatory networks upstream of the motor program underlying food intake in the Drosophila larval central nervous system. We discovered that the serotonergic network of the CNS is able to set the motor rhythm frequency of pharyngeal pumping. Pharmacological experiments verified that modulation of the feeding motor pattern is based on the release of serotonin...
July 19, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28732618/predictors-of-myopic-photorefractive-keratectomy-retreatment
#15
Russell Pokroy, Michael Mimouni, Tzahi Sela, Gur Munzer, Igor Kaiserman
PURPOSE: To determine the factors associated with retreatment after photorefractive keratectomy (PRK) in myopic eyes. SETTING: Care-Vision Laser Centers, Tel-Aviv, Israel. DESIGN: Retrospective cohort study. METHODS: A large database on myopic PRK with mitomycin-C (MMC) performed from 2005 to 2012 was studied. Patients were divided into 2 groups according to whether they had retreatment. Multiple preoperative and intraoperative parameters were analyzed for association with retreatment...
June 2017: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/28732417/expanding-spectrum-and-diversifying-role-of-laser-beam-from-intraoperative-cranial-surgery-adjunct-to-magnetic-resonance-guided-minimally-invasive-thermal-laser-ablation-therapy-for-intracranial-tumor
#16
https://www.readbyqxmd.com/read/28731623/concentric-photothermal-coagulation-with-basket-integrated-optical-device-for-treatment-of-tracheal-stenosis
#17
Van Nam Tran, Hyungshin Lee, Van Gia Truong, Yun-Hee Rhee, Hyun Wook Kang
A basket-integrated optical device is developed to consistently treat tubular tissue by centering an optical diffuser in the lumen. Four nitinol arms in conjunction with the optical diffusing applicator are deployed to induce homogeneous circumferential light emission and concentric photothermal coagulation on tracheal tissue. 1470-nm laser light is employed for the tissue testing at various irradiation conditions and evaluated in terms of thermal gradient and temperature evolution. Preliminary experiments on liver tissue demonstrate the concentric development of the radial thermal coagulation in the tissue (eccentric ratio = ~5...
July 21, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28730109/human-eye-ocular-component-analysis-for-refractive-state-and-refractive-surgery
#18
Chao-Kai Chang, Jui-Teng Lin, Yong Zhang
AIM: To analyze the clinical factors influencing the human vision corrections via the changing of ocular components of human eye in various applications; and to analyze refractive state via a new effective axial length. METHODS: An effective eye model was introduced by the ocular components of human eye including refractive indexes, surface radius (r1, r2, R1, R2) and thickness (t, T) of the cornea and lens, the anterior chamber depth (S1) and the vitreous length (S2)...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28729643/superhydrophobic-drag-reduction-spherical-bearing-fabricated-by-laser-ablation-and-pei-regulated-zno-nanowire-growth
#19
Rui Weng, Haifeng Zhang, Yanjing Tuo, Yang Wang, Xiaowei Liu
The resistance of the bearing is a significant factor affecting the performance of the ball-disk rotor gyroscope. The micro and nano combined surface with low surface energy material modifications can be hydrophobic. This can reduce the drag when the bearing is lubricated by deionized water. Laser ablation method is utilized to form micron-scaled structures on the surface of the stainless steel rotor ball. And the nanostructures are formed by PEI (Polyetherimide) regulated ZnO nanowires growth. After low surface energy material modification, the water contact angle of processed surface was 163° and the sliding angle was less than 4°...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729400/vessel-targeted-chemophototherapy-with-cationic-porphyrin-phospholipid-liposomes
#20
Dandan Luo, Jumin Geng, Nasi Li, Kevin A Carter, Shuai Shao, G Ekin Atilla-Gokcumen, Jonathan F Lovell
Cationic liposomes have been used for targeted drug delivery to tumor blood vessels, via mechanisms that are not fully elucidated. Doxorubicin (Dox)-loaded liposomes were prepared that incorporate a cationic lipid; 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), along with a small amount of porphyrin-phospholipid (PoP). Near infrared (NIR) light induced release of entrapped Dox via PoP-mediated DOTAP photo-oxidation. The formulation was optimized to enable extremely rapid NIR light-triggered Dox release (i...
July 20, 2017: Molecular Cancer Therapeutics
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