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https://www.readbyqxmd.com/read/28634334/image-guided-transplantation-of-single-cells-in-the-bone-marrow-of-live-animals
#1
Raphaël Turcotte, Clemens Alt, Judith M Runnels, Kyoko Ito, Juwell W Wu, Walid Zaher, Luke J Mortensen, Lev Silberstein, Daniel C Côté, Andrew L Kung, Keisuke Ito, Charles P Lin
Transplantation of a single hematopoietic stem cell is an important method for its functional characterization, but the standard transplantation protocol relies on cell homing to the bone marrow after intravenous injection. Here, we present a method to transplant single cells directly into the bone marrow of live mice. We developed an optical platform that integrates a multiphoton microscope with a laser ablation unit for microsurgery and an optical tweezer for cell micromanipulation. These tools allow image-guided single cell transplantation with high spatial control...
June 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28633589/laser-induced-thermal-ablation-of-cancerous-cell-organelles
#2
Renat R Letfullin, Scott A Szatkowski
AIM: By exploiting the physical changes experienced by cancerous organelles, we investigate the feasibility of destroying cancerous cells by single and multipulse modes of laser heating. MATERIALS & METHODS: Our procedure consists of two primary steps: determining the normal and cancerous organelles optical properties and simulating the heating of all of the major organelles in the cell to find the treatment modes for the laser ablation of cancerous organelles without harming healthy cells...
July 2017: Therapeutic Delivery
https://www.readbyqxmd.com/read/28633107/effects-of-chemical-and-physical-enhancement-techniques-on-transdermal-delivery-of-3-fluoroamphetamine-hydrochloride
#3
Ashana Puri, Kevin S Murnane, Bruce E Blough, Ajay K Banga
The present study investigated the passive transdermal delivery of 3-fluoroamphetamine hydrochloride (PAL-353) and evaluated the effects of chemical and physical enhancement techniques on its permeation through human skin. In vitro drug permeation studies through dermatomed human skin were performed using Franz diffusion cells. Passive permeation of PAL-353 from propylene glycol and phosphate buffered saline as vehicles was studied. Effect of oleic acid, maltose microneedles, ablative laser, and anodal iontophoresis on its transdermal permeation was investigated...
June 17, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28632988/solid-sampling-with-a-diode-laser-for-portable-ambient-mass-spectrometry
#4
Yeni P Yung, Raveendra Wickramasinghe, Anu Vaikkinen, Tiina Johanna Kauppila, Igor V Veryovkin, Luke Hanley
A handheld diode laser is implemented for solid sampling in portable ambient mass spectrometry (MS). Specifically, a pseudo-continuous wave battery-powered surgical laser diode is employed for portable laser diode thermal desorption (LDTD) at 940 nm and compared with nanosecond pulsed laser ablation at 2940 nm. Postionization is achieved in both cases using atmospheric pressure photoionization (APPI). The laser ablation atmospheric pressure photoionization (LAAPPI) and LDTD-APPI mass spectra of sage leaves (Salvia officinalis) using a field-deployable quadrupole ion trap MS display many similar ion peaks, as do the mass spectra of membrane grown biofilms of Pseudomonas aeruginosa...
June 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28630409/two-dimensional-fluorescence-spectroscopy-of-uranium-isotopes-in-femtosecond-laser-ablation-plumes
#5
Mark C Phillips, Brian E Brumfield, Nicole LaHaye, Sivanandan S Harilal, Kyle C Hartig, Igor Jovanovic
We demonstrate measurement of uranium isotopes in femtosecond laser ablation plumes using two-dimensional fluorescence spectroscopy (2DFS). The high-resolution, tunable CW-laser spectroscopy technique clearly distinguishes atomic absorption from (235)U and (238)U in natural and highly enriched uranium metal samples. We present analysis of spectral resolution and analytical performance of 2DFS as a function of ambient pressure. Simultaneous measurement using time-resolved absorption spectroscopy provides information on temporal dynamics of the laser ablation plume and saturation behavior of fluorescence signals...
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28628822/spatial-distribution-of-trace-element-ca-normalized-ratios-in-primary-and-permanent-human-tooth-enamel
#6
Théo Tacail, Lenka Kovačiková, Jaroslav Brůžek, Vincent Balter
The trace elements distribution embedded in tooth enamel offers a means to study exposure of toxic metals and allows the reconstruction of dietary behaviors. The quantification of most of the elements with a spatial high resolution (~50μm) is routinely achieved using laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS). However, the lack of a comprehensive framework of trace elements distribution in enamel jeopardizes any endorsed sampling strategy using LA-ICPMS. The present work is an effort to improve our knowledge on this issue...
June 16, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28628747/laser-ablation-of-silver-in-liquid-organic-monomer-influence-of-experimental-parameters-on-the-synthesized-silver-nanoparticles-graphite-colloids
#7
Maxime Delmée, Gregory Mertz, Julien Bardon, Adeline Marguier, Lydie Ploux, Vincent Roucoules, David Ruch
During the last decade, synthesizing silver nanoparticles (Ag NPs) by Liquid Phase-Pulsed Laser Ablation (LP-PLA) has attracted a lot of attention. Basically, this technique allows producing various metallic nanoparticles with controlled size, shape, composition or surroundings in several liquids (i.e.: water, ethanol, acetone, toluene,…). Recently, such processes have been studied in liquid organic monomer such as methyl methacrylate (MMA). However, the influence of the laser parameters on the materials synthesised in such reactive liquid and their features were not fully investigated so far...
June 19, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28628688/effectiveness-of-a-nutraceutical-during-non-ablative-1927-nm-fractional-laser-on-patients-with-facial-hyperpigmentation-and-photoaging
#8
Joely Kaufman-Janette, Alex Cazzaniga, Annelyse Ballin, Rachel Swanson-Garcell
<p>Background: Fractional lasers have been proven to treat hyperpigmentation and photoaging. Little research has been done on the effects of supplements on healing post-laser resurfacing. A nutraceutical could offer the benefit of faster healing of the skin and fewer side effects.</p> <p>Objective: Evaluate the effectiveness of a nutraceutical associated with fractional 1927 nm laser in healing time and effectiveness on hyperpigmentation and photoaging.</p> <p>Methods & Materials: A prospective, randomized, evaluator-blinded, pilot study included Fitzpatrick skin types I-III patients with hyperpigmentation and photoaging randomly assigned to two groups...
May 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28626285/selective-laser-ablation-of-carious-lesions-using-simultaneous-scanned-near-ir-diode-and-co2-lasers
#9
Kenneth H Chan, Daniel Fried
Previous studies have established that carious lesions can be imaged with high contrast using near-IR wavelengths coincident with high water absorption, namely 1450-nm, without the interference of stains. It has been demonstrated that computer-controlled laser scanning systems utilizing IR lasers operating at high pulse repetition rates can be used for serial imaging and selective removal of caries lesions. In this study, a point-to-point scanning system was developed integrating a 1450-nm diode laser with the CO2 ablation laser...
January 28, 2017: Proceedings of SPIE
https://www.readbyqxmd.com/read/28624755/stereo-vision-based-tracking-of-soft-tissue-motion-with-application-to-online-ablation-control-in-laser-microsurgery
#10
Andreas Schoob, Dennis Kundrat, Lüder A Kahrs, Tobias Ortmaier
Recent research has revealed that image-based methods can enhance accuracy and safety in laser microsurgery. In this study, non-rigid tracking using surgical stereo imaging and its application to laser ablation is discussed. A recently developed motion estimation framework based on piecewise affine deformation modeling is extended by a mesh refinement step and considering texture information. This compensates for tracking inaccuracies potentially caused by inconsistent feature matches or drift. To facilitate online application of the method, computational load is reduced by concurrent processing and affine-invariant fusion of tracking and refinement results...
June 8, 2017: Medical Image Analysis
https://www.readbyqxmd.com/read/28623990/incidence-of-venous-leg-ulcer-healing-and-recurrence-after-treatment-with-endovenous-laser-ablation
#11
William A Marston, Jason Crowner, Ana Kouri, Corey A Kalbaugh
BACKGROUND: The Effect of Surgery and Compression on Healing and Recurrence (ESCHAR) trial previously reported that patients with venous leg ulcers treated with saphenous stripping experienced a significantly reduced incidence of ulcer recurrence compared with patients treated with compression therapy. Most patients with leg ulcers and saphenous insufficiency are currently treated with endovenous thermal ablation (EVTA), but little information is available on the long-term results after EVTA in Clinical, Etiology, Anatomy, and Pathophysiology (CEAP) clinical class 5 (C5) and class 6 (C6) patients...
July 2017: Journal of Vascular Surgery. Venous and Lymphatic Disorders
https://www.readbyqxmd.com/read/28623988/rivaroxaban-for-thrombosis-prophylaxis-in-endovenous-laser-ablation-with-and-without-phlebectomy
#12
Heiko Uthoff, Daniel Holtz, Pavel Broz, Daniel Staub, Luca Spinedi
OBJECTIVE: Endovenous heat-induced thrombosis (EHIT) is a well-described complication of endovenous laser ablation (EVLA). We report our centers' experience on the efficacy (EHIT level ≥2 according to the Kabnick classification) and safety (observed major and minor bleeding events) of rivaroxaban for EHIT prophylaxis in EVLA with and without concomitant phlebectomy. METHODS: Demographic, procedural, and outcome data of all patients with EVLA of the great, accessory, or small saphenous vein and EHIT prophylaxis with 10 mg/d rivaroxaban between 2012 and 2014 were reviewed and analyzed in this investigator-initiated multicenter retrospective observational single-arm study...
July 2017: Journal of Vascular Surgery. Venous and Lymphatic Disorders
https://www.readbyqxmd.com/read/28622607/mammalian-fibroblast-cells-avoid-residual-stress-zone-caused-by-nanosecond-laser-pulses
#13
Candace Colpitts, Amin M Ektesabi, Rachael A Wyatt, Bryan D Crawford, Amirkianoosh Kiani
This study investigates the effects of laser irradiation on crystalline silicon and its application in biomaterials. We used an analytical model to predict the ablation depth and pit size resulting from laser exposure of silicon samples. The temperatures generated are predicted correlate with laser power, and to result in the formation of a residual stress zone bordering the ablated groove. Different crystal orientations found in the substrate confirm that there was crystal distortion, which consequently induces these residual stress zones...
June 9, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28621306/targeted-two-photon-chemical-apoptotic-ablation-of-defined-cell-types-in-vivo
#14
Robert A Hill, Eyiyemisi C Damisah, Fuyi Chen, Alex C Kwan, Jaime Grutzendler
A major bottleneck limiting understanding of mechanisms and consequences of cell death in complex organisms is the inability to induce and visualize this process with spatial and temporal precision in living animals. Here we report a technique termed two-photon chemical apoptotic targeted ablation (2Phatal) that uses focal illumination with a femtosecond-pulsed laser to bleach a nucleic acid-binding dye causing dose-dependent apoptosis of individual cells without collateral damage. Using 2Phatal, we achieve precise ablation of distinct populations of neurons, glia and pericytes in the mouse brain and in zebrafish...
June 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/28618498/magnetohydrodynamics-of-laser-produced-high-energy-density-plasma-in-a-strong-external-magnetic-field
#15
Kazuki Matsuo, Hideo Nagatomo, Zhe Zhang, Philippe Nicolai, Takayoshi Sano, Shohei Sakata, Sadaoki Kojima, Seung Ho Lee, King Fai Farley Law, Yasunobu Arikawa, Youichi Sakawa, Taichi Morita, Yasuhiro Kuramitsu, Shinsuke Fujioka, Hiroshi Azechi
Recent progress in the generation in the laboratory of a strong (>100-T) magnetic field enables us to investigate experimentally unexplored magnetohydrodynamics phenomena of a high-energy-density plasma, which an external magnetic field of 200-300 T notably affects due to anisotropic thermal conduction, even when the magnetic field pressure is much lower than the plasma pressure. The external magnetic field reduces electron thermal conduction across the external magnetic field lines because the Larmor radius of the thermal electrons in the external magnetic field is much shorter than the mean free path of the thermal electrons...
May 2017: Physical Review. E
https://www.readbyqxmd.com/read/28617724/intracorneal-inlays-for-the-correction-of-presbyopia
#16
Perry S Binder
The current nonsurgical approaches for the correction of presbyopia are spectacles or contact lenses or the use of pharmaceuticals to create an artificial pupil and/or to stimulate residual accommodation. Refractive surgical procedures use a monovision approach and/or a multifocal corneal laser ablation with or without the induction of negative spherical aberration to improve near vision. More recently, new surgical approaches include intracorneal inlays. Inlay approaches include increasing corneal curvature alone, implanting a multifocal inlay, or by implanting a small aperture device that functions as a pinhole to restore unaided near and intermediate visual acuity...
June 14, 2017: Eye & Contact Lens
https://www.readbyqxmd.com/read/28616953/universal-liquid-phase-laser-fabrication-of-various-nano-metals-encapsulated-by-ultrathin-carbon-shells-for-deep-uv-plasmonics
#17
Miao Yu, Chao Yang, Xiao-Ming Li, Tian-Yu Lei, Hao-Xuan Sun, Li-Ping Dai, Yu Gu, Xue Ning, Ting Zhou, Chao Wang, Hai-Bo Zeng, Jie Xiong
The exploration of localized surface plasmon resonance (LSPR) beyond the usual visible waveband, for example within the ultraviolet (UV) or deep-ultraviolet (D-UV) regions, is of great significance due to its unique applications in secret communications and optics. However, it is still challenging to universally synthesize the corresponding metal nanostructures due to their high activity. Herein, we report a universal, eco-friendly, facile and rapid synthesis of various nano-metals encapsulated by ultrathin carbon shells, significantly with a remarkable deep-UV LSPR characteristic, via a liquid-phase laser fabrication method...
June 15, 2017: Nanoscale
https://www.readbyqxmd.com/read/28616724/nd-yag-laser-therapy-for-rectal-and-vaginal-venous-malformations
#18
Lori A Gurien, Richard J Jackson, Michelle M Kiser, Gresham T Richter
BACKGROUND: Limited therapeutic options exist for rectal and vaginal venous malformations (VM). We describe our center's experience using Nd:YAG laser for targeted ablation of abnormal veins to treat mucosally involved pelvic VM. METHODS: Records of patients undergoing non-contact Nd:YAG laser therapy of pelvic VM at a tertiary children's hospital were reviewed. Symptoms, operative findings and details, complications, and outcomes were evaluated. RESULTS: Nine patients (age 0-24) underwent Nd:YAG laser therapy of rectal and/or vaginal VM...
June 14, 2017: Pediatric Surgery International
https://www.readbyqxmd.com/read/28616235/exacerbation-of-alopecia-areata-a-possible-complication-of-sodium-tetradecyl-sulphate-foam-sclerotherapy-treatment-for-varicose-veins
#19
Mark S Whiteley, Victoria C Smith
A 40-year-old woman with a history of alopecia areata related to stress or hormonal changes was treated for bilateral primary symptomatic varicose veins (CEAP clinical score C2S) of pelvic origin, using a staged procedure. Her first procedure entailed pelvic vein embolisation of three pelvic veins using 14 coils and including foam sclerotherapy of the tributaries, using 3% sodium tetradecyl sulphate. Following this procedure, she had an exacerbation of alopecia areata with some moderate shedding of hair. Subsequently, she underwent endovenous laser ablation under local anaesthetic without incident...
2017: SAGE open medical case reports
https://www.readbyqxmd.com/read/28616118/stress-urinary-incontinence-in-women-current-and-emerging-therapeutic-options
#20
REVIEW
Samer Shamout, Lysanne Campeau
Surgical management of stress urinary incontinence (SUI) is most commonly achieved by midurethral synthetic sling (MUS) insertion as a first-line surgical option. A great deal of research continues to evolve new management strategies to reach an optimal balance of high efficacy and minimal adverse events. This expert opinion review provides a brief and comprehensive discussion of recent advances and ongoing research in the management of SUI, with an emphasis on single-incision mini-slings, vaginal laser treatment, and cell-based therapy...
June 2017: Canadian Urological Association Journal, Journal de L'Association des Urologues du Canada
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