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https://www.readbyqxmd.com/read/29027626/emerging-roles-for-lpp-in-metastatic-cancer-progression
#1
REVIEW
Elaine Ngan, Alex Kiepas, Claire M Brown, Peter M Siegel
LIM domain containing proteins are important regulators of diverse cellular processes, and play pivotal roles in regulating the actin cytoskeleton. Lipoma Preferred Partner (LPP) is a member of the zyxin family of LIM proteins that has long been characterized as a promoter of mesenchymal/fibroblast cell migration. More recently, LPP has emerged as a critical inducer of tumor cell migration, invasion and metastasis. LPP is thought to contribute to these malignant phenotypes by virtue of its ability to shuttle into the nucleus, localize to adhesions and, most recently, to promote invadopodia formation...
October 13, 2017: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/29026462/murine-hepatocellular-carcinoma-derived-stem-cells-reveal-epithelial-to-mesenchymal-plasticity
#2
Aparna Jayachandran, Ritu Shrestha, Bijay Dhungel, I-Tao Huang, Marianna Yumi Kawashima Vasconcelos, Brian J Morrison, Charmaine A Ramlogan-Steel, Jason C Steel
AIM: To establish a model to enrich and characterize stem-like cells from murine normal liver and hepatocellular carcinoma (HCC) cell lines and to further investigate stem-like cell association with epithelial-to-mesenchymal transition (EMT). METHODS: In this study, we utilized a stem cell conditioned serum-free medium to enrich stem-like cells from mouse HCC and normal liver cell lines, Hepa 1-6 and AML12, respectively. We isolated the 3-dimensional spheres and assessed their stemness characteristics by evaluating the RNA levels of stemness genes and a cell surface stem cell marker by quantitative reverse transcriptase-PCR (qRT-PCR)...
September 26, 2017: World Journal of Stem Cells
https://www.readbyqxmd.com/read/29026005/low-expression-of-mir-30a-5p-induced-the-proliferation-and-invasion-of-oral-cancer-via-promoting-the-expression-of-fap
#3
Peng Ruan, Aili Tan, Zezhang Tao
The study aimed at investigating the effects of miR-30a-5p on the biological functions of oral cancer cells and figuring out the potential mechanism. We firstly verified the low expression of miR-30a-5p and high expression of FAP (Homo sapiens fibroblast activation protein alpha) in oral cancerous tissues and their negative correlation. Then, the target relationship between miR-30a-5p and FAP was validated by dual luciferase reporter assay and biotin-coupled miRNA pulldown assay. After transfection in Tca-8113 cells and SCC-15 cells, MTT, colony formation, Transwell and wound healing assays were performed to investigate how miR-30a-5p and FAP adjusted propagation, invasiveness and migration respectively...
October 12, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29025896/high-throughput-single-molecule-telomere-characterization
#4
Jennifer McCaffrey, Eleanor Young, Katy Lassahn, Justin Sibert, Steven Pastor, Harold Riethman, Ming Xiao
We have developed a novel method that enables global subtelomere and haplotype-resolved analysis of telomere lengths at the single-molecule level. An in vitro CRISPR/Cas9 RNA-directed nickase system directs the specific labeling of human (TTAGGG)n DNA tracts in genomes that have also been barcoded using a separate nickase enzyme that recognizes a 7-bp motif genome-wide. High-throughput imaging and analysis of large DNA single molecules from genomes labeled in this fashion using a nanochannel array system permits mapping through subtelomere repeat element (SRE) regions to unique chromosomal DNA while simultaneously measuring the (TTAGGG)n tract length at the end of each large telomere-terminal DNA segment...
October 12, 2017: Genome Research
https://www.readbyqxmd.com/read/29025661/low-systemic-toxicity-nanocarriers-fabricated-from-heparin-mpeg-and-pamam-dendrimers-for-controlled-drug-release
#5
Vu Minh Thanh, Thi Hiep Nguyen, Tuong Vi Tran, Uyen-Thi Phan Ngoc, Minh Nhat Ho, Thi Thinh Nguyen, Yen Nguyen Tram Chau, Van Thu Le, Ngoc Quyen Tran, Cuu Khoa Nguyen, Dai Hai Nguyen
In this report, poly(amide amine) (PAMAM) dendrimer and Heparin-grafted-monomethoxy polyethylene glycol (HEP-mPEG) were synthesized and characterized. In aqueous solution, the generation 4 PAMAM dendrimers (G4.0-PAMAM) existed as nanoparticles with particle size of 5.63nm. However, after electrostatic complexation with HEP-mPEG to form a core@shell structure G4.0-PAMAM@HEP-mPEG, the size of nanoparticles was significantly increased (73.82nm). The G4.0-PAMAM@HEP-mPEG nanoparticles showed their ability to effectively encapsulate doxorubicin (DOX) for prolonged and controlled release...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29025374/the-prognostic-significance-of-cancer-associated-fibroblasts-in-pancreatic-ductal-adenocarcinoma
#6
Hyunjin Park, Yangkyu Lee, Hyejung Lee, Jin-Won Kim, Jin-Hyeok Hwang, Jaihwan Kim, Yoo-Seok Yoon, Ho-Seong Han, Haeryoung Kim
Cancer-associated fibroblasts are abundant in the desmoplastic stroma of pancreatic ductal adenocarcinomas and are considered to play important roles in tumor progression. In this study, we investigated the expression status of secreted protein acidic and rich in cysteine, periostin, fibroblast-activated protein, and the newly developed proCOL11A1 antibody in the stroma of surgically resected pancreatic ductal adenocarcinomas and their prognostic implications. Tissue microarrays were constructed from 155 surgically resected pancreatic ductal adenocarcinomas and paired non-neoplastic pancreata and from another independent set of 48 normal/benign pancreata, and immunohistochemical stains were performed for proCOL11A1, fibroblast-activated protein, secreted protein acidic and rich in cysteine, and periostin...
October 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/29024500/tumor-suppressive-roles-of-eugenol-in-human-lung-cancer-cells
#7
Li Fangjun, Yang Zhijia
BACKGROUND: Eugenol, a natural compound available in Syzigium aromaticum (cloves), is exploited for various medicinal applications. Eugenol induces apoptosis and functions as an anti-cancer drug in several types of tumors. We investigated the tumor suppressive role and potential mechanisms of eugenol in human lung cancer cells. METHODS: Human embryonic lung fibroblast MRC-5 and lung cancer adenocarcinoma A549 cells were incubated with or without various concentrations of eugenol for 24 hours...
October 12, 2017: Thoracic Cancer
https://www.readbyqxmd.com/read/29023935/exosome-mir-335-as-a-novel-therapeutic-strategy-in-hepatocellular-carcinoma
#8
Fang Wang, Tian Hongwei, Ling Li, Klaus Piontek, Masazumi Sakaguchi, Florin M Selaru
Hepatocellular Cancer (HCC) is a common and deadly cancer. Most cases of HCC arise in a cirrhotic/fibrotic liver, raising the possibility that the environment plays a paramount role in cancer genesis. Previous studies from our group and others have shown that, in desmoplastic cancers, there is a rich intercellular communication between activated, cancer-associated fibroblasts (CAF) and cancer cells. Moreover, Extracellular Vesicles (EVs), or exosomes, were identified asan important arm of this intercellular communication platform...
October 10, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29023465/in-vitro-effects-of-apixaban-on-5-different-cancer-cell-lines
#9
Luigina Guasti, Alessandro Squizzato, Paola Moretto, Davide Vigetti, Walter Ageno, Francesco Dentali, Andrea M Maresca, Leonardo Campiotti, Anna M Grandi, Alberto Passi
BACKGROUND: Cancer is associated with hypercoagulability. However, several data suggest that anticoagulant drugs may have an effect on tumor development and progression mediated by both coagulation dependent processes and non-coagulation dependent processes. Therefore, we investigated the in vitro effects of Apixaban on cell proliferation, mortality, cell migration, gene expression and matrix metalloproteinase in 5 different cancer cell lines. METHODS: The following cancer cell lines, and 2 normal fibroblast cultures (lung and dermal fibroblasts), were studied: OVCAR3 (ovarian cancer), MDA MB 231 (breast cancer), CaCO-2 (colon cancer), LNCaP (prostate cancer) and U937 (histiocytic lymphoma)...
2017: PloS One
https://www.readbyqxmd.com/read/29023408/rational-design-of-nucleoside-bile-acid-conjugates-incorporating-a-triazole-moiety-for-anticancer-evaluation-and-sar-exploration
#10
Maria Luisa Navacchia, Elena Marchesi, Lara Mari, Nicola Chinaglia, Eleonora Gallerani, Riccardo Gavioli, Massimo Luigi Capobianco, Daniela Perrone
Herein we report a study on the synthesis and biological evaluation of a library of nucleoside-bile acid conjugates prepared by combining 2'-deoxyadenosine, 2'-deoxyguanosine, 2'-deoxyuridine as well as adenosine and guanosine derivatives with cheno-, urso-, nor-cheno-, nor-urso- and taurourso-desoxycholic acid derivatives by means of the click reaction. The new nucleoside-bile acid conjugates incorporating a triazole moiety were tested in vitro against leukemic K562 and HCT116 colon carcinoma, as well as on normal fibroblast cells...
October 12, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29022485/prevalence-of-%C3%AE-iii-tubulin-tubb3-expression-in-human-normal-tissues-and-cancers
#11
Fermín Person, Waldemar Wilczak, Claudia Hube-Magg, Christoph Burdelski, Christina Möller-Koop, Ronald Simon, Mercedes Noriega, Guido Sauter, Stefan Steurer, Susanne Burdak-Rothkamm, Frank Jacobsen
Microtubules are multifunctional cytoskeletal proteins that are involved in crucial cellular roles including maintenance of cell shape, intracellular transport, meiosis, and mitosis. Class III beta-tubulin (βIII-tubulin, also known as TUBB3) is a microtubule protein, normally expressed in cells of neuronal origin. Its expression was also reported in various other tumor types, such as several types of lung cancer, ovarian cancer, and esophageal cancer. TUBB3 is of clinical relevance as overexpression has been linked to poor response to microtubule-targeting anti-cancer drugs such as taxanes...
October 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/29022097/prevalence-of-fibroblast-growth-factor-receptor-1-fgfr1-amplification-in-squamous-cell-carcinomas-of-the-head-and-neck
#12
Till Sebastian Clauditz, Arne Böttcher, Henning Hanken, Kerstin Borgmann, Guido Sauter, Waldemar Wilczak, Tobias Grob, Adrian Münscher
BACKGROUND: FGFR1 is a receptor tyrosine kinases involved in tumor growth signaling, survival, and differentiation in many solid cancer types. There is growing evidence that FGFR1 amplification might predict therapy response to FGFR1 inhibitors in squamous cell lung cancers. To estimate the potential applicability of anti FGFR1 therapies in squamous cell carcinomas of the head and neck, we studied patterns of FGFR1 amplification using fluorescence in situ hybridization (FISH). MATERIALS AND METHODS: A tissue microarray was constructed from 453 primary treatment-naive squamous cell carcinomas of the head and neck regions with histopathological and clinical follow-up data [including oral cavity (n = 222), oropharynx (n = 126), and larynx (n = 105)]...
October 11, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/29021814/sex-specific-association-between-fibroblast-growth-factor-21-and-type-2-diabetes-a-nested-case-control-study-in-singapore-chinese-men-and-women
#13
Yeli Wang, Woon-Puay Koh, Jian-Min Yuan, An Pan
BACKGROUND: Fibroblast growth factor 21 (FGF-21) is mainly secreted by liver and has been reported to be involved in the pathogenesis of type 2 diabetes. Some prospective studies have shown a positive association between FGF-21 and diabetes risk. However, no study has examined whether the association differed by sex, which has been reported between FGF-21 and atherosclerosis. Therefore, we prospectively evaluated the sex-specific association between FGF-21 and diabetes in a Chinese population...
2017: Nutrition & Metabolism
https://www.readbyqxmd.com/read/29021221/cancer-associated-fibroblasts-promote-directional-cancer-cell-migration-by-aligning-fibronectin
#14
Begum Erdogan, Mingfang Ao, Lauren M White, Anna L Means, Bryson M Brewer, Lijie Yang, M Kay Washington, Chanjuan Shi, Omar E Franco, Alissa M Weaver, Simon W Hayward, Deyu Li, Donna J Webb
Cancer-associated fibroblasts (CAFs) are major components of the carcinoma microenvironment that promote tumor progression. However, the mechanisms by which CAFs regulate cancer cell migration are poorly understood. In this study, we show that fibronectin (Fn) assembled by CAFs mediates CAF-cancer cell association and directional migration. Compared with normal fibroblasts, CAFs produce an Fn-rich extracellular matrix with anisotropic fiber orientation, which guides the cancer cells to migrate directionally...
October 11, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28991639/haemanthamine-alters-sodium-butyrate-induced-histone-acetylation-p21-waf1-cip1-expression-chk1-and-chk2-activation-and-leads-to-increased-growth-inhibition-and-death-in-a2780-ovarian-cancer-cells
#15
Martina Seifrtová, Radim Havelek, Lucie Cahlíková, Daniela Hulcová, Naděžda Mazánková, Martina Řezáčová
BACKGROUND: Haemanthamine (HA) and sodium butyrate (NaB) are promising candidates for chemotherapy as a treatment for cancer. PURPOSE: We aimed to determine the anticancer potential of HA and NaB, alone and in combination, in A2780 ovarian cancer cells and concurrently investigated anticancer potential in contrast to non-cancer human MRC-5 fibroblasts. METHODS: Antiproliferative effects were determined by WST-1 assay and by Trypan blue exclusion staining...
November 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28990218/a-polysaccharide-from-dictyophora-indusiata-inhibits-the-immunosuppressive-function-of-cancer-associated-fibroblasts
#16
Shuyu Han, Chaoxiong Ma, Minghua Hu, Yuanyuan Wang, Fangli Ma, Ning Tao, Zhihai Qin
Reversing the function of cancer-associated fibroblasts (CAFs) may improve the efficacy of cancer therapy. Here, we isolated a novel polysaccharide from Dictyophora indusiata (ZSP4) and examined its effects on the function of prostate CAFs. The supernatant of prostate CAFs can stimulate the proliferation of immune cells and inhibit the growth of CD4+/CD8+ T cells. However, after ZSP4 stimulation, the functions of prostate CAFs were inhibited. The mechanism experiment shows that ZSP4 can stimulate prostate CAFs by down-regulating the expression of α-smooth muscle actin...
October 8, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28988933/cancer-stemness-in-bone-marrow-micrometastases-of-human-breast-cancer
#17
Marissa C Kuo, Anai N Kothari, Paul C Kuo, Zhiyong Mi
BACKGROUND: Cancer cells metastasize to the bone marrow to create the premetastatic niche. Cancer stemness (expression of stem cell characteristics) is regulated by the tumor microenvironment and associated with self-renewal and poor clinical outcomes. Osteopontin induces mesenchymal stem cells in the tumor microenvironment to adopt a cancer-associated fibroblast phenotype to potentiate cancer growth and metastasis. The mechanisms by which cancer cells and tumor microenvironment regulate stemness in the bone marrow premetastatic niche is unknown...
October 5, 2017: Surgery
https://www.readbyqxmd.com/read/28988750/design-synthesis-and-biological-evaluation-of-2-phenylquinoline-4-carboxamide-derivatives-as-a-new-class-of-tubulin-polymerization-inhibitors
#18
Li Zhu, Kaixiu Luo, Ke Li, Yi Jin, Jun Lin
A novel series of 2-phenylquinoline-4-carboxamide derivatives was synthesized, characterized and evaluated for its antiproliferative activity against five cancer cell lines, Hela, SK-OV-3, HCT116, A549 and MDA-MB-468, and a normal human fetal lung fibroblastic cell line, MRC-5. Among them, compound 7b displayed potent cytotoxic activity in vitro against SK-OV-3 and HCT116 cell lines with IC50 values of 0.5 and 0.2μM, respectively. In general, the antiproliferative activity was correlated with the binding property of the colchicine binding site and inhibitory effect on tubulin polymerization...
September 18, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28987608/spirotriazoline-oxindoles-a-novel-chemical-scaffold-with-in%C3%A2-vitro-anticancer-properties
#19
Carlos J A Ribeiro, Rute C Nunes, Joana D Amaral, Lídia M Gonçalves, Cecília M P Rodrigues, Rui Moreira, Maria M M Santos
The design and synthesis of a library of twenty-six spirotriazoline oxindoles and their in vitro evaluation as potential anticancer agents is reported. The antiproliferative activity of the synthesized compounds was assessed against four different cancer cell lines (HCT-116 p53((+/+)), HCT-116 p53((-/-)), MCF-7, and MDA-MB-231). Four spirotriazoline oxindoles showed selectivity against the four cancer cell lines tested over the non-cancer derived HEK 293T cell line. To characterize the molecular mechanisms involved in compound antitumoral activity, two spirotriazoline oxindoles were selected for further studies...
September 21, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28987603/synthesis-and-antiproliferative-properties-of-new-hydrophilic-esters-of-triterpenic-acids
#20
Barbara Eignerova, Michal Tichy, Jana Krasulova, Miroslav Kvasnica, Lucie Rarova, Romana Christova, Milan Urban, Barbara Bednarczyk-Cwynar, Marian Hajduch, Jan Sarek
To improve the properties of cytotoxic triterpenoid acids 1-5, a large set of hydrophilic esters was synthesized. We choose betulinic acid (1), dihydrobetulinic acid (2), 21-oxoacid 3 along with highly active des-E lupane acids 4 and 5 as a model set of compounds for esterification of which the properties needed to be improved. As ester moieties were used - methoxyethanol and 2-(2-methoxyethoxy)ethanol and glycolic unit (type a-d), pyrrolidinoethanol, piperidinoethanol and morpholinoethanol (type f-h), and monosaccharide groups (type i-l)...
September 21, 2017: European Journal of Medicinal Chemistry
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