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https://www.readbyqxmd.com/read/28819056/effects-of-sublethal-doses-of-thiacloprid-and-its-formulation-calypso-%C3%A2-on-the-learning-and-memory-performance-of-honey-bees
#1
Léa Tison, Sophie Holtz, Amy Adeoye, Önder Kalkan, Nina S Irmisch, Randolf Menzel
Learning and memory play a central role in behavior and communication of foraging bees. We already showed that chronic uptake of the neonicotinoid thiacloprid affects the behavior of honey bees in the field. Foraging behavior, homing success, navigation performance, and social communication were impaired. Thiacloprid collected at a feeding site at low doses accumulates in foragers over time. Here we applied a laboratory standard procedure, the proboscis extension response (PER) conditioning, in order to assess which processes, acquisition, memory consolidation and/or memory retrieval were compromised after bees were fed either with thiacloprid or the formulation of thiacloprid named Calypso(®) at 3 different sublethal doses...
August 17, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28817941/-a-case-of-severe-legionella-longbeachae-pneumonia-and-usefulness-of-lamp-assay
#2
Kumiko Matsushita, Kohei Hijikuro, Shohei Arita, Yu Kaneko, Masahiro Isozaki
Urinary antigen test is frequently used as a routine laboratory test for early diagnosis of Legionella infection, which is especially suitable for ordinary Legionella pneumophila serogroup 1, but not for other types of Legionella. We report a case of severe pneumonia caused by Legionella longbeachae, where a method of loop-mediated isothermal amplification (LAMP) assay contributed an important role for the early detection. This case involved an 83-year-old man who developed fever, dyspnea, and productive cough...
August 15, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28808447/predictors-for-mortality-among-multidrug-resistant-tuberculosis-patients-in-tanzania
#3
Edson W Mollel, Jaffu O Chilongola
PROBLEM: Factors related to MDRTB mortality in Tanzania have not been adequately explored and reported. OBJECTIVES: To determine demographic, clinical, radiographic, and laboratory factors associated with MDRTB mortality in a Tanzanian TB Referral Hospital. METHODOLOGY: This was a cross-sectional study with 193 participants. Demographic, clinical, laboratory, and radiological data were collected, and their associations with mortality among MDRTB patients were determined...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28805472/network-experiences-from-a-cross-sector-biosafety-level-3-laboratory-collaboration-a-swedish-forum-for-biopreparedness-diagnostics
#4
Johanna Thelaus, Anna Lindberg, Susanne Thisted Lambertz, Mona Byström, Mats Forsman, Hans Lindmark, Rickard Knutsson, Viveca Båverud, Andreas Bråve, Pontus Jureen, Annelie Lundin Zumpe, Öjar Melefors
The Swedish Forum for Biopreparedness Diagnostics (FBD) is a network that fosters collaboration among the 4 agencies with responsibility for the laboratory diagnostics of high-consequence pathogens, covering animal health and feed safety, food safety, public health and biodefense, and security. The aim of the network is to strengthen capabilities and capacities for diagnostics at the national biosafety level-3 (BSL-3) laboratories to improve Sweden's biopreparedness, in line with recommendations from the EU and WHO...
August 14, 2017: Health Security
https://www.readbyqxmd.com/read/28802836/neocortical-prodynorphin-expression-is-transiently-increased-with-learning-implications-for-time-and-learning-dependent-neocortical-kappa-opioid-receptor-activation
#5
Ryan Loh, Sean Collins, Roberto Galvez
There are several lines of evidence that indicate a prominent role for the opioid system in the acquisition and consolidation of learned associations. Specifically, kappa opioid receptor (KOR) modulation has been demonstrated to alter various behavioral tasks including whisker trace eyeblink conditioning (WTEB). WTEB is an associative conditioning paradigm in which a neutral conditioned stimulus (CS; Whisker stimulation) is paired following a short stimulus free trace interval with a salient unconditioned stimulus that elicits a blink response (US; Eye shock)...
August 9, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28793145/role-of-genetic-testing-in-inherited-cardiovascular-disease-a-review
#6
Allison L Cirino, Stephanie Harris, Neal K Lakdawala, Michelle Michels, Iacopo Olivotto, Sharlene M Day, Dominic J Abrams, Philippe Charron, Colleen Caleshu, Christopher Semsarian, Jodie Ingles, Harry Rakowski, Daniel P Judge, Carolyn Y Ho
Importance: Genetic testing is a valuable tool for managing inherited cardiovascular disease in patients and families, including hypertrophic, dilated, and arrhythmogenic cardiomyopathies and inherited arrhythmias. By identifying the molecular etiology of disease, genetic testing can improve diagnostic accuracy and refine family management. However, unique features associated with genetic testing affect the interpretation and application of results and differentiate it from traditional laboratory-based diagnostics...
August 9, 2017: JAMA Cardiology
https://www.readbyqxmd.com/read/28772938/a-constitutive-model-for-soft-clays-incorporating-elastic-and-plastic-cross-anisotropy
#7
Jorge Castro, Nallathamby Sivasithamparam
Natural clays exhibit a significant degree of anisotropy in their fabric, which initially is derived from the shape of the clay platelets, deposition process and one-dimensional consolidation. Various authors have proposed anisotropic elastoplastic models involving an inclined yield surface to reproduce anisotropic behavior of plastic nature. This paper presents a novel constitutive model for soft structured clays that includes anisotropic behavior both of elastic and plastic nature. The new model incorporates stress-dependent cross-anisotropic elastic behavior within the yield surface using three independent elastic parameters because natural clays exhibit cross-anisotropic (or transversely isotropic) behavior after deposition and consolidation...
May 25, 2017: Materials
https://www.readbyqxmd.com/read/28770421/evolution-of-copper-arsenate-resistance-for-enhanced-enargite-bioleaching-using-the-extreme-thermoacidophile-metallosphaera-sedula
#8
Chenbing Ai, Samuel McCarthy, Yuting Liang, Deepak Rudrappa, Guanzhou Qiu, Paul Blum
Adaptive laboratory evolution (ALE) was employed to isolate arsenate and copper cross-resistant strains, from the copper-resistant M. sedula CuR1. The evolved strains, M. sedula ARS50-1 and M. sedula ARS50-2, contained 12 and 13 additional mutations, respectively, relative to M. sedula CuR1. Bioleaching capacity of a defined consortium (consisting of a naturally occurring strain and a genetically engineered copper sensitive strain) was increased by introduction of M. sedula ARS50-2, with 5.31 and 26.29% more copper recovered from enargite at a pulp density (PD) of 1 and 3% (w/v), respectively...
August 2, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28747168/the-biobank-for-the-molecular-classification-of-kidney-disease-research-translation-and-precision-medicine-in-nephrology
#9
Daniel A Muruve, Michelle C Mann, Kevin Chapman, Josee F Wong, Pietro Ravani, Stacey A Page, Hallgrimur Benediktsson
BACKGROUND: Advances in technology and the ability to interrogate disease pathogenesis using systems biology approaches are exploding. As exemplified by the substantial progress in the personalized diagnosis and treatment of cancer, the application of systems biology to enable precision medicine in other disciplines such as Nephrology is well underway. Infrastructure that permits the integration of clinical data, patient biospecimens and advanced technologies is required for institutions to contribute to, and benefit from research in molecular disease classification and to devise specific and patient-oriented treatments...
July 26, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28745138/fluid-mud-consolidation-delayed-by-extracellular-polymer-substances
#10
Pang Qixiu, Zhang Ruibo, Wen Chunpeng, Han Peipei
A strain of bacterium identified as Burkholderia vietnamiensis was newly isolated and screened from mud deposited in a harbour basin. The effects of B. vietnamiensis on the fluid mud consolidation, and the extracellular polymer substances (EPS) secreted by the microorganisms, were examined in laboratory experiments. The experiments revealed the important characteristics of the EPS and their critical contents for nautical-depth applications. The self-weight consolidations in the settling and permeability regimes were best predicated by an exponential equation and a logarithmic equation, respectively...
July 26, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28744265/bacterial-community-dynamics-and-biocement-formation-during-stimulation-and-augmentation-implications-for-soil-consolidation
#11
Navdeep K Dhami, Walaa R Alsubhi, Elizabeth Watkin, Abhijit Mukherjee
Microbially-induced CaCO3 precipitation (MICP) is a naturally occurring process wherein durable carbonates are formed as a result of microbial metabolic activities. In recent years, MICP technology has been widely harnessed for applications in civil engineering wherein synthesis of calcium carbonate crystals occurs at ambient temperature paving way for low energy biocement. MICP using pure urease (UA) and carbonic anhydrase (CA) producing bacteria has been promising in laboratory conditions. In the current study we enriched ureolytic and carbonic anhydrase communities in calcareous soil under biostimulation and bioaugmentation conditions and investigated the effect of microbial dynamics on carbonate precipitation, calcium carbonate polymorph selection and consolidation of biological sand column under nutrient limited and rich conditions...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28690430/neurogaming-technology-meets-neuroscience-education-a-cost-effective-scalable-and-highly-portable-undergraduate-teaching-laboratory-for-neuroscience
#12
Bianca de Wit, Nicholas A Badcock, Tijl Grootswagers, Katherine Hardwick, Lina Teichmann, Jordan Wehrman, Mark Williams, David Michael Kaplan
Active research-driven approaches that successfully incorporate new technology are known to catalyze student learning. Yet achieving these objectives in neuroscience education is especially challenging due to the prohibitive costs and technical demands of research-grade equipment. Here we describe a method that circumvents these factors by leveraging consumer EEG-based neurogaming technology to create an affordable, scalable, and highly portable teaching laboratory for undergraduate courses in neuroscience...
2017: Journal of Undergraduate Neuroscience Education: JUNE: a Publication of FUN, Faculty for Undergraduate Neuroscience
https://www.readbyqxmd.com/read/28686584/assessing-recent-and-remote-associative-olfactory-memory-in-rats-using-the-social-transmission-of-food-preference-paradigm
#13
Benjamin Bessières, Olivier Nicole, Bruno Bontempi
Rats have the ability to learn about potential food sources by sampling their odors on the breath of conspecifics. Although this ethologically based social behavior has been transposed to the laboratory to probe nonspatial associative olfactory memory, only a few studies have taken full advantage of its unique features to examine the organization of recently and remotely acquired information. We provide a set of standardized procedures and technical refinements that are particularly useful in achieving this goal while minimizing confounding factors...
July 2017: Nature Protocols
https://www.readbyqxmd.com/read/28678592/orbital-tuberculosis-clinicopathological-correlation-and-diagnosis-using-pcr-in-formalin-fixed-tissues
#14
Ruchi Mittal, Savitri Sharma, Suryasnata Rath, Manas Ranjan Barik, Devjyoti Tripathy
This article describes the clinicopathological correlation and challenges encountered in the diagnosis of orbital tuberculosis (TB). Retrospective, interventional case series. A chart review of six patients who underwent biopsy for an orbital mass between January 2012 and December 2013 was performed. Institutional review board approval was obtained for the study. Clinical, radiological, and laboratory findings were documented, and response to antitubercular therapy (ATT) was noted. Age at presentation ranged from 18 to 64 years and duration of complaints varied from 2 weeks to 2 months...
July 5, 2017: Orbit
https://www.readbyqxmd.com/read/28665185/the-interplay-of-energy-balance-and-daily-timing-of-activity-in-a-subterranean-rodent-a-laboratory-and-field-approach
#15
Patricia Tachinardi, Verónica S Valentinuzzi, Gisele A Oda, C Loren Buck
The tuco-tuco (Ctenomys aff. knighti) is among the rodent species known to be nocturnal under standard laboratory conditions and diurnal under natural conditions. The circadian thermoenergetics (CTE) hypothesis postulates that switches in activity timing are a response to energetic challenges; daytime activity reduces thermoregulatory costs by consolidating activity to the warmest part of the day. Studying wild animals under both captive and natural conditions can increase understanding of how temporal activity patterns are shaped by the environment and could serve as a test of the CTE hypothesis...
September 2017: Physiological and Biochemical Zoology: PBZ
https://www.readbyqxmd.com/read/28621243/retrospective-evaluation-of-patients-with-organizing-pneumonia-is-cryptogenic-organizing-pneumonia-different-from-secondary-organizing-pneumonia
#16
Saliha Yılmaz, Berna Akıncı Özyürek, Yurdanur Erdoğan, Burcu Cirit Koçer, Funda Demirağ, Yeliz Dadalı, Sertaç Büyükyaylacı Özden
Introduction: Organizing pneumonia (OP) is an uncommon clinic opathological situation among lung diseases. If no underlying cause can be detected, it is named as cryptogenic OP (COP). In this study, the etiologic and clinical characteristics of patients diagnosed as OP in our hospital in the last ten years were evaluated retrospectively. It was also aimed to make a comparison between COP and secondary OP patients. Materials and Methods: One hundred sixty-five patients diagnosed as OP pathologically in the 10 year period from August 2003 to August 2013 were included into that study...
March 2017: Tüberküloz Ve Toraks
https://www.readbyqxmd.com/read/28610670/lung-ultrasound-as-a-diagnostic-tool-for-radiographically-confirmed-pneumonia-in-low-resource-settings
#17
Laura E Ellington, Robert H Gilman, Miguel A Chavez, Farhan Pervaiz, Julio Marin-Concha, Patricia Compen-Chang, Stefan Riedel, Shalim J Rodriguez, Charlotte Gaydos, Justin Hardick, James M Tielsch, Mark Steinhoff, Jane Benson, Evelyn A May, Dante Figueroa-Quintanilla, William Checkley
BACKGROUND: Pneumonia is a leading cause of morbidity and mortality in children worldwide; however, its diagnosis can be challenging, especially in settings where skilled clinicians or standard imaging are unavailable. We sought to determine the diagnostic accuracy of lung ultrasound when compared to radiographically-confirmed clinical pediatric pneumonia. METHODS: Between January 2012 and September 2013, we consecutively enrolled children aged 2-59 months with primary respiratory complaints at the outpatient clinics, emergency department, and inpatient wards of the Instituto Nacional de Salud del Niño in Lima, Peru...
July 2017: Respiratory Medicine
https://www.readbyqxmd.com/read/28578207/schizophrenia-a-review-of-potential-biomarkers
#18
REVIEW
Daniela Rodrigues-Amorim, Tania Rivera-Baltanás, Marta López, Carlos Spuch, Jose M Olivares, Roberto C Agís-Balboa
OBJECTIVES: Understanding the biological process and progression of schizophrenia is the first step to developing novel approaches and new interventions. Research on new biomarkers is extremely important when the goal is an early diagnosis (prediction) and precise theranostics. The objective of this review is to understand the research on biomarkers and their effects in schizophrenia to synthesize the role of these new advances. METHODS: In this review, we search and review publications in databases in accordance with established limits and specific objectives...
October 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/28573578/structure-determination-using-x-ray-free-electron-laser-pulses
#19
Henry N Chapman
The intense X-ray pulses from free-electron lasers, of only femtoseconds duration, outrun most of the processes that lead to structural degradation in X-ray exposures of macromolecules. Using these sources it is therefore possible to increase the dose to macromolecular crystals by several orders of magnitude higher than usually tolerable in conventional measurements, allowing crystal size to be decreased dramatically in diffraction measurements and without the need to cool the sample. Such pulses lead to the eventual vaporization of the sample, which has required a measurement approach, called serial crystallography, of consolidating snapshot diffraction patterns of many individual crystals...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28525630/laboratory-integration-and-consolidation-in-a-regional-health-system
#20
Jim Cook
Health systems face intense pressure to decrease costs and improve services as the health care delivery system in the United States undergoes tremendous change due to health care reform. As health systems grow, like any business, they are forced to explore standardization to realize and maintain efficient practices. Clinical services, such as laboratory medicine, are more difficult to integrate due to wider variation in acceptable practice and culture, compared with other services. However, changes to laboratory service are imperative if health care professionals expect to survive and thrive in the new business environment...
May 19, 2017: Laboratory Medicine
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