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https://www.readbyqxmd.com/read/28551741/soil-calcium-significantly-promotes-uptake-of-inorganic-arsenic-by-garland-chrysanthemum-chrysanthemuml-coronarium-fertilized-with-chicken-manure-bearing-roxarsone-and-its-metabolites
#1
Lixian Yao, Lianxi Huang, Cuihua Bai, Zhaohuan He, Changmin Zhou
Roxarsone (ROX), a widely used feed organoarsenic additive, occurs as itself and its metabolites in animal manure that is commonly land used as fertilizer. Soil property impacts arsenic (As) speciation and bioavailability. Fourteen soils across China were used to conduct culture experiments to investigate As uptake by garland chrysanthemum (ChrysanthemumL coronarium), with the soils fertilized with chicken manure bearing ROX and its metabolites. The results show As(III) was the sole As form in garland chrysanthemum shoots, and As(III) and As(V) occurred in roots...
May 27, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28551483/effect-of-growth-hormone-on-steroid-concentrations-and-mrna-expression-of-their-receptor-and-selected-egg-specific-protein-genes-in-the-chicken-oviduct-during-pause-in-laying-induced-by-fasting
#2
J K Socha, A Sechman, M Mika, A Hrabia
This study was undertaken to examine the effect of growth hormone (GH) treatment during pause in laying on (1) the concentration of steroids in blood plasma and oviduct tissues, (2) the expression of mRNA of steroid receptors, and (3) the mRNA expression of selected egg-specific proteins in the chicken oviduct. A pause in egg laying was induced by food deprivation for 5 d, followed by feeding every other day, and then feeding daily from Day 10 onward. Birds were divided into three groups: control (n = 18) fed ad libitum, subjected to pause in laying (n = 18), and subjected to pause in laying and injected every day with 200 μg/kg BW of chicken GH (chGH; n = 18)...
May 8, 2017: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/28551413/applying-the-chicken-embryo-chorioallantoic-membrane-assay-to-study-treatment-approaches-in-urothelial-carcinoma
#3
Margaretha A Skowron, Anuja Sathe, Andrea Romano, Michèle J Hoffmann, Wolfgang A Schulz, Gommert A van Koeveringe, Peter Albers, Roman Nawroth, Günter Niegisch
BACKGROUND: Rapid development of novel treatment options demands valid preclinical screening models for urothelial carcinoma (UC). The translational value of high-throughput drug testing using 2-dimensional (2D) cultures is limited while for xenograft models handling efforts and costs often become prohibitive for larger-scale drug testing. Therefore, we investigated to which extent the chicken chorioallantoic membrane (CAM) assay might provide an alternative model to study antineoplastic treatment approaches for UC...
May 25, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/28551263/identification-of-a-chicken-gallus-gallus-endogenous-reference-gene-actb-and-its-application-in-meat-adulteration
#4
Wenjin Xiang, Ying Shang, Qin Wang, Yuancong Xu, Pengyu Zhu, Kunlun Huang, Wentao Xu
The genes commonly used to determine meat species are mainly mitochondrial, but the copy numbers of such genes are high, meaning they cannot be accurately quantified. In this paper, for the first time, the chromosomal gene Actb was selected as an endogenous reference gene for chicken species. It was assayed in four different chicken varieties and 16 other species using both qualitative and quantitative PCR. No amplification of the Actb gene was found in species other than chicken and no allelic variations were detected in chicken...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28550720/development-and-validation-of-an-uplc-uv-method-for-determination-of-a-novel-triazine-coccidiostat-ethanamizuril-and-its-metabolite-m3-in-chicken-tissues
#5
Xiao Zhao, Youfa Xu, Lifang Zhang, Chunmei Wang, Chunna Guo, Chenzhong Fei, Keyu Zhang, Xiaoyang Wang, Yingchun Liu, Mi Wang, Wenli Zheng, Feiqun Xue
Ethanamizuril (EZL) is a novel anti-coccidial triazine synthesized by the Shanghai Veterinary Research Institute of the Chinese Academy of Agricultural Sciences. As a novel coccidiostat, it is essential to study its residue in food animal tissues. In this study, a reliable UPLC method for the determination of ethanamizuril (EZL) and one of its metabolites (M3) in chicken muscle, skin and fat, liver, and kidney samples was developed. Analytes were extracted using acetonitrile and 10% sodium carbonate solution, defatted by n-hexane, and further purified by using an Oasis MCX cartridge...
May 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28550677/mineral-profile-of-children-s-fast-food-menu-samples
#6
Maria Luisa Cervera, Miguel de la Guardia, Manuela Ruiz-de-Cenzano, Arancha Rochina-Marco, Óscar López-Salazar
Children's fast food menus, including hamburgers served with french fries, dessert, and a soft drink, were analyzed to obtain the mineral profile of trace elements. The developed analytical methodology involved sample digestion under pressure inside a microwave oven with a mixture of HNO₃ and H₂O₂ and inductively coupled plasma-optical emission spectrometry. The method was validated by carrying out the analysis of certified reference materials (NIST 1570a spinach leaves, NCS ZC73016 chicken, and NIST 1568a rice flour) and using recovery experiments...
May 26, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28550391/gut-liver-axis-cirrhosis-and-portal-hypertension-the-chicken-and-the-egg
#7
Juan P Arab, Rosa M Martin-Mateos, Vijay H Shah
The term gut-liver axis is used to highlight the close anatomical and functional relationship between the intestine and the liver. The intestine has a highly specialized epithelial membrane which regulates transport across the mucosa. Due to dysbiosis, impairment of the intestinal barrier and altered immunity status, bacterial products can reach the liver through the portal vein, where they are recognized by specific receptors, activate the immune system and lead to a proinflammatory response. Gut microbiota and bacterial translocation play an important role in the pathogenesis of chronic liver diseases, including alcoholic and non-alcoholic fatty liver disease, cirrhosis, and its complications, such as portal hypertension, spontaneous bacterial peritonitis and hepatic encephalopaty...
May 26, 2017: Hepatology International
https://www.readbyqxmd.com/read/28549257/human-somatic-cells-deficient-for-rad52-are-impaired-for-viral-integration-and-compromised-for-most-aspects-of-homology-directed-repair
#8
Yinan Kan, Nizar N Batada, Eric A Hendrickson
Homology-directed repair (HDR) maintains genomic integrity by eliminating lesions such as DNA double-strand breaks (DSBs), interstrand crosslinks (ICLs) and stalled replication forks and thus a deficiency in HDR is associated with genomic instability and cancer predisposition. The mechanism of HDR is best understood and most rigorously characterized in yeast. The inactivation of the fungal radiation sensitive 52 (RAD52) gene, which has both recombination mediator and single-strand annealing (SSA) activities in vitro, leads to severe HDR defects in vivo...
May 10, 2017: DNA Repair
https://www.readbyqxmd.com/read/28548864/oral-administration-of-a-medium-containing-both-d-aspartate-producing-live-bacteria-and-d-aspartate-reduces-rectal-temperature-in-chickens
#9
P H Do, P V Tran, M A Bahry, H Yang, G Han, A Tsuchiya, Y Asami, M Furuse, V S Chowdhury
1. The aim of this study was to investigate the effects on the rectal temperature of young chickens of the oral administration of a medium that contained both live bacteria that produce D-aspartate (D-Asp) and D-Asp. 2. In Experiment 1, chickens were subjected to chronic oral administration of either the medium (containing live bacteria and 2.46 μmol D-Asp) or water from 7 to 14 d of age. Plasma-free amino acids as well as mitochondrial biogenic gene expression in the breast muscle were analysed. In Experiment 2, 7-d-old chickens were subjected to acute oral administration of the above medium or of an equimolar amount of D-Asp to examine their effect on changes in rectal temperature...
May 26, 2017: British Poultry Science
https://www.readbyqxmd.com/read/28548568/genetic-antigenic-and-pathogenic-characteristics-of-newcastle-disease-viruses-isolated-from-geese-in-china
#10
Qianqian Xu, Junfeng Sun, Mengying Gao, Shasha Zhao, Huairan Liu, TingTing Zhang, Zongxi Han, Xiangang Kong, Shengwang Liu
Four Newcastle disease virus (NDV) strains were isolated from domestic, commercial geese that showed clinical signs that were believed to be the result of NDV infections. The genetic, antigenic, and pathogenic characteristics of the 4 NDVs were compared with those of NDV strains that were isolated from chickens. The complete genomes of 2 of the NDV strains contained 15,186 nucleotides (nt); the other 2 contained 15,192 nt, and exhibited the typical genomic organization of genotype II NDV and molecular characteristics of VIId NDVs...
May 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28548038/marek-s-disease-virus-infection-of-phagocytes-a-de-novo-in-vitro-infection-model
#11
Pankaj Chakraborty, Lonneke Vervelde, Robert G Dalziel, Peter S Wasson, Venugopal Nair, Bernadette M Dutia, Pete Kaiser
Marek's disease virus (MDV) is an alphaherpesvirus that induces T-cell lymphomas in chickens. Natural infections in vivo are caused by the inhalation of infected poultry house dust and it is presumed that MDV infection is initiated in the macrophages from where the infection is passed to B cells and activated T cells. Virus can be detected in B and T cells and macrophages in vivo, and both B and T cells can be infected in vitro. However, attempts to infect macrophages in vitro have not been successful. The aim of this study was to develop a model for infecting phagocytes [macrophages and dendritic cells (DCs)] with MDV in vitro and to characterize the infected cells...
May 26, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28547997/effect-of-purine-nucleosides-on-growth-performance-gut-morphology-digestive-enzymes-serum-profile-and-immune-response-in-broiler-chickens
#12
A Daneshmand, H Kermanshahi, M Danesh Mesgaran, A J King, S A Ibrahim
1. This study was conducted to evaluate the effects of purine nucleosides on performance, gut morphology, intestinal enzymes and immunity functions in broiler chickens from 0 to 21 d of age. 2. A total of 360 1-d-old male chickens (Cobb 500) were randomly assigned to 4 treatments with 6 replications. Experimental diets consisted of a control without any additives and diets containing 0.1% pure adenosine, 0.1% pure guanosine and 0.1% equal aliquots of pure adenosine and guanosine. Two birds per cage (12 birds per treatment) were killed on d 11 and 21 in order to obtain serum samples for lipid profile, jejunal samples for morphology and mucosal immunity, digestive enzymes for epithelial maturation, and bursa and spleen samples for relative weight of immune organs to live body weight...
May 26, 2017: British Poultry Science
https://www.readbyqxmd.com/read/28546882/in-situ-synthesis-of-a-magnetic-graphene-platform-for-the-extraction-of-benzimidazoles-from-food-samples-and-analysis-by-high-performance-liquid-chromatography
#13
Qianchun Zhang, Yulan Liu, Xingyi Wang, Huimin Li, Junyu Chen
A novel method was proposed for the determination of five benzimidazoles (oxfendazole, mebendazole, flubendazole, albendazole, and fenbendazole) using magnetic graphene (G-Fe3O4). G-Fe3O4 was synthesized via in situ chemical coprecipitation. The properties of G-Fe3O4 were characterized by various instrumental methods. G-Fe3O4 exhibited a great adsorption ability and good stability towards analytes. Various experimental parameters that might affect the extraction efficiency such as the amount of G-Fe3O4, extraction solvent, extraction time, and desorption conditions were evaluated...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28545903/potential-of-fecal-waste-for-the-production-of-biomethane-bioethanol-and-biodiesel
#14
Mohamed A Gomaa, Raeid M M Abed
Fecal waste is an environmental burden that requires proper disposal, which ultimately becomes also an economic burden. Because fecal waste is nutrient-rich and contains a diverse methanogenic community, it has been utilized to produce biomethane via anaerobic digestion. Carbohydrates and lipids in fecal waste could reach up to 50% of the dry weight, which also suggests a potential as a feedstock for bioethanol and biodiesel production. We measured biomethane production from fecal waste of cows, chickens, goats and humans and compared the microbial community composition before and after anaerobic digestion...
May 22, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28543398/interdigital-tissue-remodelling-in-the-embryonic-limb-involves-dynamic-regulation-of-the-mirna-profiles
#15
Beatriz Garcia-Riart, Carlos I Lorda-Diez, Jessica C Marin-Llera, Juan A Garcia-Porrero, Juan M Hurle, Juan A Montero
Next-generation sequencing in combination with quantitative polymerase chain reaction analysis revealed a dynamic miRNA signature in the interdigital mesoderm of the chick embryonic hinlimb in the course of interdigit remodelling. During this period, 612 previously known chicken miRNAs (gga-miRNAs) and 401 non-identified sequences were expressed in the interdigital mesoderm. Thirty-six microRNAs, represented by more than 750 reads per million, displayed differential expression between stages HH29 (6 id) and HH32 (7...
May 24, 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/28542335/interventions-that-effectively-target-anopheles-funestus-mosquitoes-could-significantly-improve-control-of-persistent-malaria-transmission-in-south-eastern-tanzania
#16
Emmanuel W Kaindoa, Nancy S Matowo, Halfan S Ngowo, Gustav Mkandawile, Arnold Mmbando, Marcelina Finda, Fredros O Okumu
Malaria is transmitted by many Anopheles species whose proportionate contributions vary across settings. We re-assessed the roles of Anopheles arabiensis and Anopheles funestus, and examined potential benefits of species-specific interventions in an area in south-eastern Tanzania, where malaria transmission persists, four years after mass distribution of long-lasting insecticide-treated nets (LLINs). Monthly mosquito sampling was done in randomly selected households in three villages using CDC light traps and back-pack aspirators, between January-2015 and January-2016, four years after the last mass distribution of LLINs in 2011...
2017: PloS One
https://www.readbyqxmd.com/read/28542327/efficient-up-conversion-in-yb-er-nat-xo4-2-thermal-nanoprobes-imaging-of-their-distribution-in-a-perfused-mouse
#17
Carlos Zaldo, María Dolores Serrano, Xiumei Han, Concepción Cascales, Marta Cantero, Lluís Montoliu, Elvira Arza, Valeria R Caiolfa, Moreno Zamai
Yb and Er codoped NaT(XO4)2 (T = Y, La, Gd, Lu and X = Mo, W) disordered oxides show a green (Er3+ related) up-conversion (UC) efficiency comparable to that of Yb:Er:β-NaYF4 compound and unless 3 times larger UC ratiometric thermal sensitivity. The similar UC efficiency of Yb:Er doped NaT(XO4)2 and β-NaYF4 compounds allowed testing equal subcutaneous depths of ex-vivo chicken tissue in both cases. This extraordinary behavior for NaT(XO4)2 oxides with large cutoff phonon energy (ħω≈ 920 cm-1) is ascribed to 4F9/2 electron population recycling to higher energy 4G11/2 level by a phonon assisted transition...
2017: PloS One
https://www.readbyqxmd.com/read/28541756/genetic-diversity-of-mtdna-d-loop-sequences-in-four-native-chinese-chicken-breeds
#18
H W Guo, C Li, X N Wang, Z J Li, G R Sun, G X Li, X J Liu, X T Kang, R L Han
1. To explore the genetic diversity of Chinese indigenous chicken breeds, a 585 bp fragment of the mitochondrial DNA (mtDNA) region was sequenced in 102 birds from the Xichuan Black-bone chicken, Yunyang Black-bone chicken and Lushi chicken. In addition, 30 mtDNA D-loop sequences of Silkie fowls were downloaded from NCBI. The mtDNA D-loop sequence polymorphism and maternal origin of 4 chicken breeds were analysed in this study. 2. The results showed that a total of 33 mutation sites and 28 haplotypes were detected in the 4 chicken breeds...
May 25, 2017: British Poultry Science
https://www.readbyqxmd.com/read/28540396/-anaphylactic-reactions-to-vaccines-chicken-egg-allergy-and-the-influenza-h1n1-vaccination
#19
L Klimek, S Wicht-Langhammer, L von Bernus, C Thorn, D Cazan, O Pfaar, K Hörmann
BACKGROUND: Allergic reactions to influenza vaccinations are a well-known phenomenon; however, only a few published statistics give information on the risks. Patients with a chicken egg allergy must be particularly carefully handled during an influenza epidemic where vaccines produced using embryonated chicken eggs are primarily employed. METHODS: A selective literature review and analysis of the problem of allergic reactions to vaccinations was performed. RESULTS: The two most common relative contraindications among patients to be vaccinated are the presence of an egg allergy and a previous anaphylactic reaction to vaccination...
May 24, 2017: HNO
https://www.readbyqxmd.com/read/28539940/horizontal-and-vertical-distribution-of-heavy-metals-in-farm-produce-and-livestock-around-lead-contaminated-goldmine-in-dareta-and-abare-zamfara-state-northern-nigeria
#20
O E Orisakwe, O O Oladipo, G C Ajaezi, N A Udowelle
Background. Hitherto studies in response to the June 2010 lead poisoning, Zamfara State, Nigeria, have focused on clinical interventions without information on livestock and other metals. Objective. This study has investigated the distribution of heavy metals in farm produce and livestock around lead-contaminated goldmine in Dareta and Abare, Zamfara State, Nigeria. Methods. Vegetables, soil, water, blood, and different meat samples were harvested from goat, sheep, cattle, and chicken from Dareta, Abare, and Gusau communities...
2017: Journal of Environmental and Public Health
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