keyword
MENU ▼
Read by QxMD icon Read
search

protein balance

keyword
https://www.readbyqxmd.com/read/27914707/adiponectin-alters-renal-calcium-and-phosphate-excretion-through-regulation-of-klotho-expression
#1
Joseph M Rutkowski, Johanne Pastor, Kai Sun, Sun K Park, I Alexandru Bobulescu, Christopher T Chen, Orson W Moe, Philipp E Scherer
The kidney controls systemic calcium and phosphate levels and disturbances of its control mechanisms can lead to a variety of diseases. The insulin-sensitizing adipokine adiponectin is renoprotective and accelerates functional recovery following renal injury. However, unlike other adipokines, adiponectin is reduced in obesity. High adiponectin levels are also correlated with bone loss, suggestive of an additional action in mineral metabolism. Using knockout, wild-type, and adiponectin-overexpressing transgenic mice, we sought to identify the mechanistic basis for adiponectin's ability to regulate calcium and phosphate balance at the level of the kidney...
November 30, 2016: Kidney International
https://www.readbyqxmd.com/read/27913573/thyroid-hormones-induce-browning-of-white-fat
#2
Noelia Martinez, José María Moreno-Navarrete, Cristina Contreras, Eva Rial-Pensado, Johan Fernø, Rubén Nogueiras, Carlos Diéguez, José M Fernández-Real, Miguel López
The canonical view about the effect of thyroid hormones (THs) on thermogenesis assumes that the hypothalamus acts merely as a modulator of the sympathetic outflow on brown adipose tissue (BAT). Recent data have challenged that vision by demonstrating that THs act on the ventromedial nucleus of the hypothalamus (VMH) to inhibit AMP-activated protein kinase (AMPK), which regulates the thermogenic program in BAT, leading to increased thermogenesis and weight loss. Current data have shown that in addition to activation of brown fat, the browning of white adipose tissue (WAT) might be also an important thermogenic mechanism...
December 2, 2016: Journal of Endocrinology
https://www.readbyqxmd.com/read/27913296/regulation-of-skeletal-muscle-insulin-stimulated-signaling-through-the-mek-redd1-mtor-axis
#3
Cory M Dungan, David L Williamson
Recent findings in adipocytes suggest that mitogen-activated protein kinase (MAPK)/extracellular-regulated signaling kinase (ERK) kinase 1/2 (MEK1/2) signaling regulates regulated in development and DNA damage 1 (REDD1) protein expression. Similarly, our previous work show that a lack of REDD1 protein expression, and associated hyperactive basal mechanistic target of rapamycin (mTOR) signaling, limits skeletal muscle's response to insulin. Therefore, we sought to determine: 1) if MEK1/2 inhibition is sufficient to reduce REDD1 protein expression and subsequently insulin receptor substrate-1 (IRS-1) tyrosine phosphorylation via negative feedback of hyperactive mTOR in REDD1 wild-type (WT) mice and 2) if rapamycin-mediated mTOR inhibition is sufficient to improve IRS-1 tyrosine phosphorylation in REDD1 knockout (KO) mice...
November 29, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27912029/how-to-establish-a-bioregenerative-life-support-system-for-long-term-crewed-missions-to-the-moon-or-mars
#4
Yuming Fu, Leyuan Li, Beizhen Xie, Chen Dong, Mingjuan Wang, Boyang Jia, Lingzhi Shao, Yingying Dong, Shengda Deng, Hui Liu, Guanghui Liu, Bojie Liu, Dawei Hu, Hong Liu
To conduct crewed simulation experiments of bioregenerative life support systems on the ground is a critical step for human life support in deep-space exploration. An artificial closed ecosystem named Lunar Palace 1 was built through integrating efficient higher plant cultivation, animal protein production, urine nitrogen recycling, and bioconversion of solid waste. Subsequently, a 105-day, multicrew, closed integrative bioregenerative life support systems experiment in Lunar Palace 1 was carried out from February through May 2014...
December 2, 2016: Astrobiology
https://www.readbyqxmd.com/read/27911717/extracellular-matrix-endocytosis-in-controlling-matrix-turnover-and-beyond-emerging-roles-in-cancer
#5
REVIEW
Elena Rainero
The extracellular matrix (ECM) is a network of secreted proteins that, beyond providing support for tissues and organs, is involved in the regulation of a variety of cell functions, including cell proliferation, polarity, migration and oncogenic transformation. ECM homeostasis is maintained through a tightly controlled balance between synthesis, deposition and degradation. While the role of metalloproteases in ECM degradation is widely recognised, the contribution of ECM internalisation and intracellular degradation to ECM maintenance has been mostly overlooked...
October 15, 2016: Biochemical Society Transactions
https://www.readbyqxmd.com/read/27910861/paper-based-crp-monitoring-devices
#6
Shang-Chi Lin, Chung-Yuh Tseng, Po-Liang Lai, Min-Yen Hsu, Shueh-Yao Chu, Fan-Gang Tseng, Chao-Min Cheng
Here, we discuss the development of a paper-based diagnostic device that is inexpensive, portable, easy-to-use, robust, and capable of running simultaneous tests to monitor a relevant inflammatory protein for clinical diagnoses i.e. C-reactive protein (CRP). In this study, we first attempted to make a paper-based diagnostic device via the wax printing method, a process that was used in previous studies. This device has two distinct advantages: 1) reduced manufacturing and assay costs and operation duration via using wax printing method to define hydrophobic boundaries (for fluidic devices or general POC devices); and, 2) the hydrophilicity of filter paper, which is used to purify and chromatographically correct interference caused by whole blood components with a tiny amount of blood sample (only 5 μL)...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910786/bioavailability-and-digestibility-of-nutrients-from-the-dried-oyster-culinary-medicinal-mushroom-pleurotus-ostreatus-agaricomycetes-in-vivo-experiments
#7
Julita Regula, Joanna Suliburska, Marek Siwulski
There is a limited number of publications on the bioavailability and digestibility of nutrients contained in macrofungi. The aim of this study was to assess the bioavailability and digestibility of macronutrients using in vivo experiments on laboratory animals. The experiments were conducted with the commercial oyster mushroom Pleurotus ostreatus. Semisynthetic diets were prepared based on the modified AIN-93M diet and were supplemented with 4% and 8% mushroom powder. Between days 4 and 13, apparent digestibility indexes were determined for all animals using the conventional balance method...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27909292/crystal-structures-of-the-tric-trimeric-intracellular-cation-channel-orthologues
#8
Go Kasuya, Masahiro Hiraizumi, Andrés D Maturana, Kaoru Kumazaki, Yuichiro Fujiwara, Keihong Liu, Yoshiko Nakada-Nakura, So Iwata, Keisuke Tsukada, Tomotaka Komori, Sotaro Uemura, Yuhei Goto, Takanori Nakane, Mizuki Takemoto, Hideaki E Kato, Keitaro Yamashita, Miki Wada, Koichi Ito, Ryuichiro Ishitani, Motoyuki Hattori, Osamu Nureki
Ca(2+) release from the sarcoplasmic reticulum (SR) and endoplasmic reticulum (ER) is crucial for muscle contraction, cell growth, apoptosis, learning and memory. The trimeric intracellular cation (TRIC) channels were recently identified as cation channels balancing the SR and ER membrane potentials, and are implicated in Ca(2+) signaling and homeostasis. Here we present the crystal structures of prokaryotic TRIC channels in the closed state and structure-based functional analyses of prokaryotic and eukaryotic TRIC channels...
December 2016: Cell Research
https://www.readbyqxmd.com/read/27909227/castration-alters-protein-balance-following-high-frequency-muscle-contraction
#9
Jennifer L Steiner, David H Fukuda, Michael L Rossetti, Jay R Hoffman, Bradley S Gordon
Resistance exercise increases muscle mass by shifting protein balance in favor of protein accretion. Androgens independently alter protein balance, but it is unknown whether androgens alter this measure following resistance exercise. To answer this, male mice were subjected to sham or castration surgery 7-8 weeks prior to undergoing a bout of unilateral, high frequency, electrically induced muscle contractions in the fasted or refed state. Puromycin was injected 30 min prior to sacrifice to measure protein synthesis...
December 1, 2016: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27907077/transcriptome-and-multivariable-data-analysis-of-corynebacterium-glutamicum-under-different-dissolved-oxygen-conditions-in-bioreactors
#10
Yang Sun, Wenwen Guo, Fen Wang, Feng Peng, Yankun Yang, Xiaofeng Dai, Xiuxia Liu, Zhonghu Bai
Dissolved oxygen (DO) is an important factor in the fermentation process of Corynebacterium glutamicum, which is a widely used aerobic microbe in bio-industry. Herein, we described RNA-seq for C. glutamicum under different DO levels (50%, 30% and 0%) in 5 L bioreactors. Multivariate data analysis (MVDA) models were used to analyze the RNA-seq and metabolism data to investigate the global effect of DO on the transcriptional distinction of the substance and energy metabolism of C. glutamicum. The results showed that there were 39 and 236 differentially expressed genes (DEGs) under the 50% and 0% DO conditions, respectively, compared to the 30% DO condition...
2016: PloS One
https://www.readbyqxmd.com/read/27906624/influence-of-4-pyridone-3-carboxamide-1%C3%AE-d-ribonucleoside-4pyr-on-activities-of-extracellular-enzymes-in-endothelial-human-cells
#11
I Pelikant-Małecka, A Sielicka, E Kaniewska, R T Smoleński, E M Słomińska
Previous studies demonstrated that human endothelial cells were capable to phosphorylate 4-pyridone-3-carboxamide-1β-D-ribonucleoside (4PYR) to monophosphate (4PYMP) and formed another metabolite-an analog of NAD (4PYRAD). Elevated levels of 4PYMP and 4PYRAD had an adverse effect on energy balance-depressed adenosine triphosphate (ATP) and nicotinamide adenine dinucleotide (NAD) concentration in human endothelial cells. Ecto-enzymes such as ecto-nucleoside triphosphate diphosphohydrolase (eNTPD); ecto-5'-nucleotidase (e5'NT); and ecto-adenosine deaminase (eADA) are involved in controlling of inflammation and platelet aggregation...
December 2016: Nucleosides, Nucleotides & Nucleic Acids
https://www.readbyqxmd.com/read/27906092/alterations-to-mtorc1-signaling-in-the-skeletal-muscle-differentially-affect-whole-body-metabolism
#12
Maitea Guridi, Barbara Kupr, Klaas Romanino, Shuo Lin, Denis Falcetta, Lionel Tintignac, Markus A Rüegg
BACKGROUND: The mammalian target of rapamycin complex 1 (mTORC1) is a central node in a network of signaling pathways controlling cell growth and survival. This multiprotein complex integrates external signals and affects different nutrient pathways in various organs. However, it is not clear how alterations of mTORC1 signaling in skeletal muscle affect whole-body metabolism. RESULTS: We characterized the metabolic phenotype of young and old raptor muscle knock-out (RAmKO) and TSC1 muscle knock-out (TSCmKO) mice, where mTORC1 activity in skeletal muscle is inhibited or constitutively activated, respectively...
March 21, 2016: Skeletal Muscle
https://www.readbyqxmd.com/read/27904674/bmi1-plays-an-important-role-in-dentin-and-mandible-homeostasis-by-maintaining-redox-balance
#13
Ying Yin, Xian Xue, Qian Wang, Ning Chen, Dengshun Miao
To explore whether polycomb repressor Bmi1 plays an important role in dentin and mandible development homeostasis by maintaining redox balance, 3-week-old Bmi1 gene knockout (Bmi1(-/-)) mice were treated with the antioxidant N-acetylcysteine (NAC) for 2 weeks in their drinking water and phenotypes of the tooth and mandibles were compared with vehicle-treated Bmi1(-/-) mice and wild-type mice by radiograph, histochemistry and immunohistochemistry. Alterations of oxidative stress, DNA damage, cell proliferation and cell cycle-related parameters were also examined in mandibles...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904150/regulating-the-balance-between-necroptosis-apoptosis-and-inflammation-by-inhibitors-of-apoptosis-proteins
#14
REVIEW
Lazaros Vasilikos, Lisanne M Spilgies, Janin Knop, WWei-Lynn Wong
Understanding how Inhibitors of APoptosis proteins (IAPs) regulate apoptosis and necroptosis has been fast-forwarded by the use of Smac mimetics to deplete or inhibit the IAPs, specifically cIAP1& 2 and XIAP. The loss or inhibition of cIAP1, cIAP2 and XIAP causes the majority of cells to be sensitized to death receptor induced cell death, particularly tumour necrosis factor (TNF). Mouse genetics shows that there is some functional redundancy and the use of Smac mimetics has allowed us to understand how changing the composition of proteins recruited to TNF receptor 1 upon TNF ligation can alter protein complex formation and activation of apoptosis or necroptosis, particularly when caspases are inhibited...
December 1, 2016: Immunology and Cell Biology
https://www.readbyqxmd.com/read/27903833/meal-distribution-of-dietary-protein-and-leucine-influences-long-term-muscle-mass-and-body-composition-in-adult-rats
#15
Layne E Norton, Gabriel J Wilson, Christopher J Moulton, Donald K Layman
BACKGROUND: Protein quantity and quality at a meal affect muscle protein synthesis (MPS); however, long-term effects of protein distribution at individual meals on adult muscle mass remain unknown. OBJECTIVE: We used a precise feeding protocol in adult rats to determine if optimizing postmeal MPS response by modifying the meal distribution of protein, and the amino acid leucine (Leu), would affect muscle mass. METHODS: Two studies were conducted with the use of male Sprague-Dawley rats (∼300 g) trained to consume 3 meals/d, then assigned to diet treatments with identical macronutrient contents (16% of energy from protein, 54% from carbohydrates, and 30% from fat) but differing in protein quality or meal distribution...
November 30, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27903408/correlations-between-ceruloplasmin-lactoferrin-and-myeloperoxidase-in-meconium
#16
Ewa Skarżyńska, Joanna Żytyńska-Daniluk, Barbara Lisowska-Myjak
BACKGROUND AND AIMS: Oxidative stress and the generation of reactive oxygen/nitrogen species has a known significant impact on intrauterine fetal growth and the risk of metabolic diseases in adulthood. Compounds accumulated in fetal meconium may be a source of information about the oxidoreductive status during the intrauterine development. Three metal-containing proteins ceruloplasmin (CP), lactoferrin (LF) and myeloperoxidase (MPO) constitute the complementary panel modulating oxidative stress...
November 23, 2016: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/27902892/microrna-145-influences-the-balance-of-th1-th2-via-regulating-runx3-in-asthma-patients
#17
Linxia Fan, Xiaojun Wang, Linlan Fan, Qizhang Chen, Hong Zhang, Hui Pan, Aixia Xu, Hongjuan Wang, Yang Yu
AIM OF THE STUDY: To delineate the underlying mechanism of microRNA-145 modulate the balance of Th1/Th2 via targeting RUNX3 in asthma patients. MATERIALS AND METHODS: Peripheral blood samples were collected from asthma patients and healthy controls. CD4(+) T cells were isolated and cultured. Using quantitative PCR detect, the level of microRNA-145 and RUNX3 mRNA level in the CD4(+) T cells from asthma patients and healthy controls, meanwhile, western blot was used to detect the RUNX3 protein level...
November 30, 2016: Experimental Lung Research
https://www.readbyqxmd.com/read/27902743/itraq-based-quantitative-proteomic-analysis-of-spirulina-platensis-in-response-to-low-temperature-stress
#18
Qingye Li, Rong Chang, Yijun Sun, Bosheng Li
Low temperature (LT) is one of the most important abiotic stresses that can significantly reduce crop yield. To gain insight into how Spirulina responds to LT stress, comprehensive physiological and proteomic analyses were conducted in this study. Significant decreases in growth and pigment levels as well as excessive accumulation of compatible osmolytes were observed in response to LT stress. An isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomics approach was used to identify changes in protein abundance in Spirulina under LT...
2016: PloS One
https://www.readbyqxmd.com/read/27902388/the-exploration-of-risk-factors-of-concurrent-bacteremia-among-critically-ill-patients-with-severe-dengue
#19
Chin-Ming Chen, Khee-Siang Chan, Kuo-Chen Cheng, Willy Chou, Hui-Chun Chao, Chiu-Yin Yeh, Wen-Liang Yu
We investigated the clinical features of intensive care unit (ICU) patients with a concomitant severe dengue infection and bacteremia to identify risk factors for this comorbidity. The records of all ICU dengue patients admitted during a July 31-November 31, 2015 were reviewed. Patients with "concurrent bacteremia" (positive bacterial blood culture within 72 hours of ICU admission) were identified. ICU admission was required for 142 patients; 22 (15.5%) had concurrent bacteremia. Streptococcus spp. was the most common pathogen, then Escherichia coli, and then Staphylococcus spp...
November 7, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27902385/motor-coordination-and-balance-measurements-reveal-differential-pathogenicity-of-currently-spreading-ev71-strains-in-human-scarb2-transgenic-mice
#20
Mei-Feng Chen, Shin-Ru Shih
Enterovirus 71 (EV71) has caused large-scale epidemics with neurological complications in Asia Pacific region. The C4a and B5 strains are the two major genotypes circulalted in many coutries recently. This study provides a new protocol, a motor coordination task, to assess the differential pathogenicity of C4a and B5 strains in human SCARB2 transgenic mice. We found that the pathogenicity of C4a viruses was more severe than that of B5 viruses. Moreover, we discovered that an increased level of monocyte chemoattractant protein-1 is positively correlated with severely deficient motor function...
October 20, 2016: Journal of General Virology
keyword
keyword
115169
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"