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Walquiria Batista Andrade, Jeferson Lucas Jacinto, Douglas Kratki da Silva, Mirela Casonato Roveratti, José Maria Estoche, Douglas Benedito Oliveira, Mario Carlos Welin Balvedi, Rubens A da Silva, Andreo Fernando Aguiar
The purpose of this study was to investigate the effects of L-arginine (L-arg) supplementation on muscle recovery process after a single session of high-intensity resistance exercise (RE). 20 healthy young adult (22.8 ± 3.4 y) participants were assigned to two groups (N = 10/group): a placebo-supplement group (PLA) or a L-arginine-supplement group (ARG). The groups completed a session of high-intensity RE (0h), and three subsequent fatigue tests sessions (at 24, 48 and 72 h post-exercise) to assess the time course of muscle recovery...
March 15, 2018: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
F A Khan, T S Chenier, R A Foster, J Hewson, E L Scholtz
Emerging research suggests that the nitric oxide system may play a role in persistent breeding-induced endometritis (PBIE) in the mare. Differences in uterine nitric oxide (NO) levels between mares susceptible or resistant to PBIE and a dose-dependent inhibitory effect of NO on uterine contractility have been demonstrated. The objectives of this study were to investigate the difference in total nitric oxide synthase (NOS) activity of the endometrium between susceptible and resistant mares and the effect of a specific inducible nitric oxide synthase (iNOS) inhibitor on the endometrial NOS activity in vitro...
March 14, 2018: Reproduction in Domestic Animals, Zuchthygiene
Nhat Tri Vo, Astam Kumar Patra, Dukjoon Kim
The new class of silica nanoparticles with unprecedented structural morphology is synthesized by hydrolysis of tetraethyl orthosilicate (TEOS) in the presence of cetyltrimethylammonium bromide (CTAB), L-arginine, and ammonium metatungstate (AMT) composite template, all in aqueous ethanol. The morphology of the synthesized mesoporous silica nanoparticles (MSNs) can be tuned from a spherical to a hollow dough-nut shape through a hollow sphere by controlling the concentration of AMT in the composite template. The formation mechanism of the hol-low doughnut shaped MSNs (hd-MSNs) is well explored by means of zeta potential, high resolution transmission electron microscopy (HRTEM) with elemental mapping analysis, and X-ray photoelectron spectroscopy...
March 13, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Naeem Khan, Asghari Bano, Mohammad Atikur Rahman, Bala Rathinasabapathi, Md Ali Babar
Genetic improvement for drought tolerance in chickpea requires a solid understanding of biochemical processes involved with different physiological mechanisms. The objective of this study is to demonstrate genetic variations in altered metabolic levels in chickpea varieties (tolerant and sensitive) grown under contrasting water regimes through UPLC-HRMS based untargeted metabolomic profiling. Chickpea plants were exposed to drought stress at the three-leaf stage for 25 days and the leaves were harvested at 14 days and 25 days after the imposition of drought stress...
March 13, 2018: Plant, Cell & Environment
Nandkishor R Kotagale, Mir Touseef Ali, Chandrabhan T Chopde, Milind J Umekar, Brijesh G Taksande
Nicotine abstinence following chronic exposure is associated with impairments in memory and variety of cognitive functions. Daily nicotine (2 mg/kg, sc, four times daily) administration for 14 days and its abrupt withdrawal significantly impaired avoidance learning in inhibitory avoidance task as indicated by a significant decrease in the step through latency. Animals injected with agmatine (10-40 μg/rat, icv) from day 7 to 14 before the first daily dose of nicotine (2 mg/kg, sc) showed increased step through latencies during retrieval test...
March 9, 2018: Pharmacology, Biochemistry, and Behavior
Youmei Wang, Minghua Lu, Dianping Tang
A new photoluminescence (PL) enzyme immunoassay was designed for sensitive detection of aflatoxin B1 (AFB1 ) via an innovative enzyme substrate, 6-aza-2-thiothymine-stabilized gold nanocluster (AAT-AuNC) with L-arginine. The enzyme substrate with strong PL intensity was formed through supramolecular host-guest assembly between guanidine group of L-arginine and AAT capped on the surface of AuNC. Upon arginase introduction, the captured L-arginine was hydrolyzed into ornithine and urea, thus resulting in the decreasing PL intensity...
March 5, 2018: Biosensors & Bioelectronics
M Tavares-Silva, D Ferreira, S Cardoso, A R Raimundo, J Barbosa-Breda, A Leite-Moreira, A Rocha-Sousa
Urocortin 2 (UCN2) is a peptide related to corticotropin-releasing factor, capable of activating CRF-R2. Among its multisystemic effects, it has actions in all 3 muscle subtypes. This study's aim was to determine its potential role in two of the intrinsic eye muscle kinetics. Strips of iris sphincter (rabbit) and ciliary (bovine) muscles were dissected and mounted in isometric force-transducer systems filled with aerated-solutions. Contraction was elicited using carbachol (10(-6) M for iris sphincter, 10(-5) M for ciliary muscle), prior to all testing substances...
March 12, 2018: Physiological Research
Scott K Ferguson, Julie W Harral, David I Pak, Katherine M Redinius, Kurt R Stenmark, Dominik J Schaer, Paul W Buehler, David C Irwin
Free hemoglobin (Hb) associated with hemolysis extravasates into vascular tissue and depletes nitric oxide (NO), which leads to impaired vascular function and could impair skeletal muscle metabolic control during exercise. We tested the hypothesis that: 1) free Hb would extravasate into skeletal muscle tissue, reducing the contracting skeletal muscle O2 delivery/O2 utilization ratio (microvascular PO2 , PO2 mv) to a similar extent as that observed following NO synthase (NOS) blockade, and 2) that the Hb scavenging protein haptoglobin (Hp) would prevent Hb extravasation and inhibit these skeletal muscle tissue effects...
March 8, 2018: Nitric Oxide: Biology and Chemistry
Zhongjian Cheng, Xinggui Shen, Xiaohua Jiang, Huimin Shan, Maria Cimini, Pu Fang, Yong Ji, Joon Young Park, Konstantinos Drosatos, Xiaofeng Yang, Christopher G Kevil, Raj Kishore, Hong Wang
Insufficient hydrogen sulfide (H2 S) has been implicated in Type 2 diabetic mellitus (T2DM) and hyperhomocysteinemia (HHcy)-related cardiovascular complications. We investigated the role of H2 S in T2DM and HHcy-induced endothelial dysfunction in small mesenteric artery (SMA) of db/db mice fed a high methionine (HM) diet. HM diet (8 weeks) induced HHcy in both T2DM db/db mice and non-diabetic db/+ mice (total plasma Hcy: 48.4 and 31.3 µM, respectively), and aggravated the impaired endothelium-derived hyperpolarization factor (EDHF)-induced endothelium-dependent relaxation to acetylcholine (ACh), determined by the presence of eNOS inhibitor N(ω)-nitro-L-arginine methyl ester (L-NAME) and prostacyclin (PGI2 ) inhibitor indomethacin (INDO), in SMA from db/db mice but not that from db/+ mice...
February 14, 2018: Redox Biology
Harikesh Dubey, Kavita Gulati, Arunabha Ray
The present study evaluated the effects of s-nitrosoglutathione (GSNO), a nitrosothiol and sustained NO releaser, on experimental model of sporadic Alzheimer`s disease (sAD) in rats. Levels of Aβ40, Aβ42 and BDNF were assessed in brain hippocampal homogenates for correlative purposes. Intracerebroventricular-Streptozotocin (icv-STZ) induced increased escape latencies (acquisition) and reduced time in target quadrant (probe trial) in Morris Water Maze (MWM) test at 3 months post icv-STZ administration. These behavioural changes were associated with increased Aβ depositions and lowered BDNF levels in brain hippocampal homogenates...
March 6, 2018: Neurotoxicology
Rezeda A Akhmadishina, Ruslan Garifullin, Natalia V Petrova, Marat I Kamalov, Timur I Abdullin
Although delocalized lipophilic cations have been identified as effective cellular and mitochondrial carriers for a range of natural and synthetic drug molecules, little is known about their effects on pharmacological properties of peptides. The effect of triphenylphosphonium (TPP) cation on bioactivity of antioxidant tetrapeptides based on the model opioid YRFK motif was studied. Two tetrapeptide variants with L-arginine (YRFK) and D-arginine (YrFK) were synthesized and coupled with carboxyethyl-TPP (TPP-3) and carboxypentyl-TPP (TPP-6) units...
2018: Frontiers in Pharmacology
Rebecca S Hesterberg, John L Cleveland, Pearlie K Epling-Burnette
The immune system is remarkably responsive to a myriad of invading microorganisms and provides continuous surveillance against tissue damage and developing tumor cells. To achieve these diverse functions, multiple soluble and cellular components must react in an orchestrated cascade of events to control the specificity, magnitude and persistence of the immune response. Numerous catabolic and anabolic processes are involved in this process, and prominent roles for l-arginine and l-glutamine catabolism have been described, as these amino acids serve as precursors of nitric oxide, creatine, agmatine, tricarboxylic acid cycle intermediates, nucleotides and other amino acids, as well as for ornithine, which is used to synthesize putrescine and the polyamines spermidine and spermine...
March 8, 2018: Medical Sciences: Open Access Journal
Chloe R McDonald, Lindsay S Cahill, Joel R Gamble, Robyn Elphinstone, Lisa M Gazdzinski, Kathleen J Y Zhong, Adrienne C Philson, Mwayiwawo Madanitsa, Linda Kalilani-Phiri, Victor Mwapasa, Feiko O Ter Kuile, John G Sled, Andrea L Conroy, Kevin C Kain
Reducing adverse birth outcomes due to malaria in pregnancy (MIP) is a global health priority. However, there are few safe and effective interventions. l-Arginine is an essential amino acid in pregnancy and an immediate precursor in the biosynthesis of nitric oxide (NO), but there are limited data on the impact of MIP on NO biogenesis. We hypothesized that hypoarginemia contributes to the pathophysiology of MIP and that l-arginine supplementation would improve birth outcomes. In a prospective study of pregnant Malawian women, we show that MIP was associated with lower concentrations of l-arginine and higher concentrations of endogenous inhibitors of NO biosynthesis, asymmetric and symmetric dimethylarginine, which were associated with adverse birth outcomes...
March 7, 2018: Science Translational Medicine
James G Beeson, Michelle J L Scoullar, Philippe Boeuf
Decreased l-arginine and nitric oxide bioavailability in pregnant women with malaria contributes to low birth weight, suggesting that l-arginine supplementation could be a potential treatment (McDonald et al ).
March 7, 2018: Science Translational Medicine
Carla Blum-Johnston, Richard B Thorpe, Chelsea Wee, Raechel Opsahl, Monica Romero, Samuel Murray, Alexander Brunelle, Quintin Blood, Rachael Wilson, Arlin B Blood, Lubo Zhang, Lawrence D Longo, William J Pearce, Sean M Wilson
Bradykinin-induced activation of the pulmonary endothelium triggers a rise in intracellular Ca2+ , which activates nitric oxide (NO)-dependent vasorelaxation. Chronic hypoxia is commonly associated with increased pulmonary vascular tone, which can cause pulmonary hypertension in responsive individuals. In the present study, we tested the hypothesis that long-term high altitude hypoxia (LTH) diminishes bradykinin-induced Ca2+ signals and inhibits eNOS, PGI2 and BKCa channels in sheep, which are moderately responsive to LTH, resulting in decreased pulmonary arterial vasorelaxation...
March 7, 2018: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Tracy Bell, Magali Araujo, Zaiming Luo, James Tomlinson, James Leiper, William J Welch, Christopher S Wilcox
BACKGROUND: Nitric oxide prevents hypertension yet paradoxically reduces proximal tubule Na+ reabsorption. Nitric oxide synthase is inhibited by asymmetric dimethylarginine (ADMA) that is metabolized by dimethylarginine dimethylaminohydrolase (DDAH) whose type 1 isoform is expressed abundantly in the PT. HYPOTHESIS: That ADMA generated by DDAH-1 fluid reabsorbtion (Jv) by the proximal tubule. METHODS: S2 segments of the PT were perfused in vivo to assess Jv...
March 7, 2018: American Journal of Physiology. Renal Physiology
Masakazu Kotoda, Tadahiko Ishiyama, Kazuha Mitsui, Sohei Hishiyama, Takashi Matsukawa
PURPOSE: Nicorandil has dual properties and acts as a nitric oxide donor and an ATP-sensitive potassium (KATP ) channel opener. Considering its pharmacological profile, nicorandil might exert protective effects on the brain as well as on the heart. The purpose of this study was to directly evaluate the effect of nicorandil on cerebral blood flow (CBF) in mice using a transcranial Doppler method. METHODS: Under general anesthesia, the nicorandil groups received a single-bolus intraperitoneal injection of the respective doses of nicorandil (1, 5, or 10 mg/kg), while the control group received vehicle only...
March 5, 2018: Journal of Anesthesia
Young Dong Yoo, Su Ran Mun, Chang Hoon Ji, Ki Woon Sung, Keum Young Kang, Ah Jung Heo, Su Hyun Lee, Jee Young An, Joonsung Hwang, Xiang-Qun Xie, Aaron Ciechanover, Bo Yeon Kim, Yong Tae Kwon
The conjugation of amino acids to the protein N termini is universally observed in eukaryotes and prokaryotes, yet its functions remain poorly understood. In eukaryotes, the amino acid l-arginine (l-Arg) is conjugated to N-terminal Asp (Nt-Asp), Glu, Gln, Asn, and Cys, directly or associated with posttranslational modifications. Following Nt-arginylation, the Nt-Arg is recognized by UBR boxes of N-recognins such as UBR1, UBR2, UBR4/p600, and UBR5/EDD, leading to substrate ubiquitination and proteasomal degradation via the N-end rule pathway...
March 5, 2018: Proceedings of the National Academy of Sciences of the United States of America
Yi-Xuan Guo, Hai-Tao Nie, Chen-Jie Xu, Guo-Min Zhang, Ling-Wei Sun, Ting-Ting Zhang, Zhen Wang, Xu Feng, Pei-Hua You, Feng Wang
The aim of this study was to determine whether nutrient restriction and arginine treatment affect energy metabolism changes and oxidative stress through the mitochondrial pathway in the ovarian tissue of ewes during the luteal phase. On days 6-15 of the estrous cycle, 24 multiparous Hu sheep (BW = 43.56 ± 1.53 kg) were randomly assigned to three groups: control group (CG; n = 6), restriction group (RG; n = 9), and l-arginine group (AG; n = 9) administered Arg treatment (or vehicle) three times per day...
February 22, 2018: Theriogenology
Minlu Han, Mengyu Xie, Jun Han, Daoyi Yuan, Tian Yang, Ying Xie
A validated liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of D- and L-amino acids in human serum. Under the optimum conditions, except for DL-proline, L-glutamine, and D-lysine, the enantioseparation of the other 19 enantiomeric pairs of proteinogenic amino acids and nonchiral glycine was achieved with a CROWNPAK CR-I(+) chiral column within 13 min. The lower limits of quantitation for L-amino acids (including glycine) and D-amino acids were 5-56.25 μM and 0...
March 1, 2018: Analytical and Bioanalytical Chemistry
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