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Serum Cobalamin

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https://www.readbyqxmd.com/read/27893837/cell-type-specific-modulation-of-cobalamin-uptake-by-bovine-serum
#1
Hua Zhao, Kalani Ruberu, Hongyun Li, Brett Garner
Tracking cellular 57Co-labelled cobalamin (57Co-Cbl) uptake is a well-established method for studying Cbl homeostasis. Previous studies established that bovine serum is not generally permissive for cellular Cbl uptake when used as a supplement in cell culture medium, whereas supplementation with human serum promotes cellular Cbl uptake. The underlying reasons for these differences are not fully defined. In the current study we address this question. We extend earlier observations by showing that fetal calf serum inhibits cellular 57Co-Cbl uptake by HT1080 cells (a fibrosarcoma-derived fibroblast cell line)...
2016: PloS One
https://www.readbyqxmd.com/read/27864850/hyperhomocysteinemia-in-greyhounds-and-its-association-with-hypofolatemia-and-other-clinicopathologic-variables
#2
R M Heilmann, N Grützner, M C Iazbik, R Lopes, C S Bridges, J S Suchodolski, C G Couto, J M Steiner
BACKGROUND: Folate and cobalamin are essential cofactors for homocysteine (HCY) metabolism. Hyperhomocysteinemia, a multifactorial condition, may reflect B vitamin deficiency and is associated with increased risk of cardiovascular disease, thrombosis, and neurodegenerative and chronic gastrointestinal diseases in humans. Hyperhomocysteinemia has been reported in Greyhounds with suspected chronic enteropathy. OBJECTIVES: To evaluate the frequencies of and the association between hypofolatemia and hyperhomocysteinemia in Greyhounds...
November 19, 2016: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/27736023/b12-deficiency-is-common-in-infants-and-is-accompanied-by-serious-neurological-symptoms
#3
T Irevall, I Axelsson, E Naumburg
AIM: Adverse neurological symptoms have been linked to vitamin B12 deficiency in infants. This explorative study described the clinical presentation associated with vitamin B12 deficiency in this age group. METHODS: The study comprised infants who were born between 2004 and 2012 and were tested for vitamin B12 levels after they were admitted to a hospital with neurological symptoms at less than one year of age. Vitamin B12 deficiency was defined as low cobalamin in serum and/or increased homocysteine and/or increased methylmalonate...
October 13, 2016: Acta Paediatrica
https://www.readbyqxmd.com/read/27730072/study-of-vitamin-b12-deficiency-and-peripheral-neuropathy-in-metformin-treated-early-type-2-diabetes-mellitus
#4
Rudra Prasad Roy, Kaushik Ghosh, Manas Ghosh, Amitava Acharyya, Ambarish Bhattacharya, Mrinal Pal, Sisir Chakraborty, Nilanjan Sengupta
BACKGROUND: Long-term therapy with metformin was shown to decrease the Vitamin B12 level and manifested as peripheral neuropathy. AIM: The aim of this study is to define the prevalence of Vitamin B12 deficiency in early Type 2 diabetic patients (duration ≤5 years or drug treatment ≤3 years) and the relationship among metformin exposure and levels of cobalamin (Cbl), folic acid, and homocysteine (Hcy) with severity of peripheral neuropathy. METHODOLOGY: This is a cross-sectional study involving randomly selected ninety patients (male 56, female 34) between age groups of 35 and 70 years, comparing those who had received >6 months of metformin (Group A) (n = 35) with those without metformin (Group B) (n = 35) and patients taking metformin with other oral hypoglycemic agent (Group C) (n = 20)...
September 2016: Indian Journal of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27641602/feline-exocrine-pancreatic-insufficiency-a-retrospective-study-of-150-cases
#5
P G Xenoulis, D L Zoran, G T Fosgate, J S Suchodolski, J M Steiner
BACKGROUND: Little information is available about the clinical presentation and response to treatment of cats with exocrine pancreatic insufficiency (EPI). OBJECTIVES: To describe the signalment, clinical signs, concurrent diseases, and response to treatment of cats with EPI. ANIMALS: One hundred and fifty cats with EPI. METHODS: Retrospective case series. RESULTS: Questionnaires were sent to 261 veterinarians, and 150 (57%) were returned with data suitable for statistical analysis...
November 2016: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/27606644/urinary-levels-of-early-kidney-injury-molecules-in-children-with-vitamin-b12-deficiency
#6
Ali Güneş, Fesih Aktar, İlhan Tan, Murat Söker, Ünal Uluca, Hasan Balık, Nuriye Mete
The aim of this study was to investigate urine early kidney injury molecules, including human kidney injury molecule-1 (KIM-1), liver-type fatty-acid binding protein (L-FABP), N-acetyl-b-D-glucosaminidase A (NAG), and neutrophil gelatinase-associated lipocalin (NGAL) in children with vitamin B12 (cobalamin) deficiency (CD). Twelve children with vitamin B12 deficiency and 20 healthy matched controls were included. Hematologic parameters, serum urea, creatinine (Cr), electrolytes, B12 and folate levels were recorded...
October 1, 2016: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/27567143/kinetic-studies-on-the-reaction-of-cob-ii-alamin-with-hypochlorous-acid-evidence-for-one-electron-oxidation-of-the-metal-center-and-corrin-ring-destruction
#7
Rohan S Dassanayake, Mohamed M Farhath, Jacob T Shelley, Soumitra Basu, Nicola E Brasch
Kinetic and mechanistic studies on the reaction of a major intracellular vitamin B12 form, cob(II)alamin (Cbl(II)), with hypochlorous acid/hypochlorite (HOCl/OCl(-)) have been carried out. Cbl(II) (Co(II)) is rapidly oxidized by HOCl to predominately aquacobalamin/hydroxycobalamin (Cbl(III), Co(III)) with a second-order rate constant of 2.4×10(7)M(-1)s(-1) (25.0°C). The stoichiometry of the reaction is 1:1. UHPLC/HRMS analysis of the product mixture of the reaction of Cbl(II) with 0.9mol equiv. HOCl provides support for HOCl being initially reduced to Cl and subsequent H atom abstraction from the corrin macrocycle occurring, resulting in small amounts of corrinoid species with two or four H atoms fewer than the parent cobalamin...
July 15, 2016: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/27538863/serum-cobalamin-vitamin-b12-concentrations-in-rhesus-macaques-macaca-mulatta-and-pigtailed-macaques-macaca-nemestrina-with-chronic-idiopathic-diarrhea
#8
Jessica M Izzi, Sarah E Beck, Robert J Adams, Kelly A Metcalf Pate, Eric K Hutchinson
Chronic diarrhea poses a significant threat to the health of NHP research colonies, and its primary etiology remains unclear. In macaques, the clinical presentation of intractable diarrhea and weight loss that are accompanied by inflammatory infiltrates within the gastrointestinal tract closely resembles inflammatory bowel disease of humans, dogs, and cats, in which low serum and tissue cobalamin (vitamin B12) levels are due to intestinal malabsorption. We therefore hypothesized that macaques with chronic idiopathic diarrhea (CID) have lower serum cobalamin concentrations than do healthy macaques...
2016: Comparative Medicine
https://www.readbyqxmd.com/read/27446930/biomarkers-and-algorithms-for-the-diagnosis-of-vitamin-b12-deficiency
#9
REVIEW
Luciana Hannibal, Vegard Lysne, Anne-Lise Bjørke-Monsen, Sidney Behringer, Sarah C Grünert, Ute Spiekerkoetter, Donald W Jacobsen, Henk J Blom
Vitamin B12 (cobalamin, Cbl, B12) is an indispensable water-soluble micronutrient that serves as a coenzyme for cytosolic methionine synthase (MS) and mitochondrial methylmalonyl-CoA mutase (MCM). Deficiency of Cbl, whether nutritional or due to inborn errors of Cbl metabolism, inactivate MS and MCM leading to the accumulation of homocysteine (Hcy) and methylmalonic acid (MMA), respectively. In conjunction with total B12 and its bioactive protein-bound form, holo-transcobalamin (holo-TC), Hcy, and MMA are the preferred serum biomarkers utilized to determine B12 status...
2016: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/27384539/analytical-bias-exceeding-desirable-quality-goal-in-4-out-of-5-common-immunoassays-results-of-a-native-single-serum-sample-external-quality-assessment-program-for-cobalamin-folate-ferritin-thyroid-stimulating-hormone-and-free-t4-analyses
#10
Gunn B B Kristensen, Pål Rustad, Jens P Berg, Kristin M Aakre
BACKGROUND: We undertook this study to evaluate method differences for 5 components analyzed by immunoassays, to explore whether the use of method-dependent reference intervals may compensate for method differences, and to investigate commutability of external quality assessment (EQA) materials. METHODS: Twenty fresh native single serum samples, a fresh native serum pool, Nordic Federation of Clinical Chemistry Reference Serum X (serum X) (serum pool), and 2 EQA materials were sent to 38 laboratories for measurement of cobalamin, folate, ferritin, free T4, and thyroid-stimulating hormone (TSH) by 5 different measurement procedures [Roche Cobas (n = 15), Roche Modular (n = 4), Abbott Architect (n = 8), Beckman Coulter Unicel (n = 2), and Siemens ADVIA Centaur (n = 9)]...
September 2016: Clinical Chemistry
https://www.readbyqxmd.com/read/27381013/the-prevalence-of-cobalamin-deficiency-among-vegetarians-assessed-by-serum-vitamin-b12-a-review-of-literature
#11
R Pawlak, S E Lester, T Babatunde
No abstract text is available yet for this article.
July 2016: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/27350907/holotranscobalamin-holotc-active-b12-and-herbert-s-model-for-the-development-of-vitamin-b12-deficiency-a-review-and-alternative-hypothesis
#12
REVIEW
Paul Henry Golding
The concentration of total vitamin B12 in serum is not a sufficiently sensitive or specific indicator for the reliable diagnosis of vitamin B12 deficiency. Victor Herbert proposed a model for the staged development of vitamin B12 deficiency, in which holotranscobalamin (HoloTC) is the first indicator of deficiency. Based on this model, a commercial immunoassay has been controversially promoted as a replacement for the total vitamin B12 test. HoloTC is cobalamin (vitamin B12) attached to the transport protein transcobalamin, in the serum, for delivery to cells for metabolism...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27329747/infection-susceptibility-in-gastric-intrinsic-factor-vitamin-b12-defective-mice-is-subject-to-maternal-influences
#13
Lynda Mottram, Anneliese O Speak, Reza M Selek, Emma L Cambridge, Zoe McIntyre, Leanne Kane, Subhankar Mukhopadhyay, Carolyn Grove, Amy Colin, Cordelia Brandt, Maria A Duque-Correa, Jessica Forbester, Tu Anh Pham Nguyen, Christine Hale, George S Vasilliou, Mark J Arends, Brendan W Wren, Gordon Dougan, Simon Clare
UNLABELLED: Mice harboring a mutation in the gene encoding gastric intrinsic factor (Gif), a protein essential for the absorption of vitamin B12/cobalamin (Cbl), have potential as a model to explore the role of vitamins in infection. The levels of Cbl in the blood of Gif(tm1a/tm1a) mutant mice were influenced by the maternal genotype, with offspring born to heterozygous (high Cbl, F1) mothers exhibiting a significantly higher serum Cbl level than those born to homozygous (low Cbl, F2) equivalents...
2016: MBio
https://www.readbyqxmd.com/read/27306031/does-canine-inflammatory-bowel-disease-influence-gut-microbial-profile-and-host-metabolism
#14
Jia Xu, Adronie Verbrugghe, Marta Lourenço, Geert P J Janssens, Daisy J X Liu, Tom Van de Wiele, Venessa Eeckhaut, Filip Van Immerseel, Isabel Van de Maele, Yufeng Niu, Guido Bosch, Greet Junius, Brigitte Wuyts, Myriam Hesta
BACKGROUND: Inflammatory bowel disease (IBD) refers to a diverse group of chronic gastrointestinal diseases, and gut microbial dysbiosis has been proposed as a modulating factor in its pathogenesis. Several studies have investigated the gut microbial ecology of dogs with IBD but it is yet unclear if this microbial profile can alter the nutrient metabolism of the host. The aim of the present study was to characterize the faecal bacterial profile and functionality as well as to determine host metabolic changes in IBD dogs...
2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27265434/therapeutic-trial-of-cobalamin-in-patients-with-normal-serum-cobalamin-levels-and-predicted-cobalamin-deficiency
#15
S Sami Kartı, Siret Ratıp, Mustafa Yılmaz, Kubilay Ukınç, Asım Örem, Cafer Adıgüzel, Faysal Dane, Nurten Kandemir, Ercüment Ovalı, Mahmut Bayık
Diagnosis of cobalamin deficiency can be difficult due to the variation in clinical presentation and lack of specificity of the laboratory findings. Although hypersegmentation and macrocytosis are important findings observed in cobalamin deficiency they are not sensitive and spesific. Additionally, cobalamin assays in commercial laboratories, being not reliable, makes the diagnosis more difficult. The metabolite assays, such as the total homocysteine and methylmalonic acid are costly, which restricts their widespread routine use...
June 5, 2003: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
https://www.readbyqxmd.com/read/27263738/autonomic-nervous-system-dysfunction-and-serum-levels-of-neurotoxic-and-neurotrophic-cytokines-in-patients-with-cobalamin-deficiency
#16
Özcan Çeneli, Şahika Zeynep Akı, Betül Çevik Küçük, Ayşe Bora Tokçaer, Reha Kuruoğlu, Münci Yağcı
OBJECTIVE: The imbalance between neurotoxic cytokine tumor necrosis factor-α (TNF-α) and neurotrophic cytokines epidermal growth factor (EGF) and interleukin-6 (IL-6) plays a role in the pathogenesis of cobalamin (Cbl) deficiency-induced neuropathy. The aim of this study was to evaluate autonomic nervous system dysfunction and to look for any relationship between autonomic nervous system disturbances and serum cytokine levels (TNF-α, EGF, IL-6) in patients with Cbl deficiency. METHODS: Serum levels of TNF-α, EGF and IL-6 were studied in patients with Cbl deficiency (n=41) and a healthy control group (n=17) and after 3 months in patients who underwent Cbl replacement therapy (n=22)...
December 5, 2010: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
https://www.readbyqxmd.com/read/27263524/therapeutic-trial-of-cobalamin-in-patients-with-normal-serum-cobalamin-levels-and-predicted-cobalamin-deficiency
#17
Burhan Ferhanoğlu
No abstract text is available yet for this article.
December 5, 2003: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
https://www.readbyqxmd.com/read/27155006/false-low-holotranscobalamin-levels-in-a-patient-with-a-novel-tcn2-mutation
#18
Peter Keller, Janine Rufener, Christof Schild, Sergey N Fedosov, Peter H Nissen, Ebba Nexo
BACKGROUND: Measurement of holotranscobalamin (holoTC) is increasingly used as a screening test for cobalamin (Cbl) deficiency. A level well below the reference interval strongly supports a deficient state. We examined a 21-year-old woman diagnosed as Cbl deficient because of an extremely low holoTC level as measured by the Abbott Architect Assay. METHODS: The patient was evaluated for Cbl deficiency employing an in-house holoTC method as well as other routine markers of Cbl status...
May 6, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/27153102/analytic-validation-of-commercially-available-immunoassays-for-the-measurement-of-serum-cobalamin-and-folate-concentrations-in-pigs
#19
Niels Grützner, Darrel Knabe, Bruce D Lawhorn, Brandon Dominguez, Johannes Kauffold, Jan S Suchodolski, Jörg M Steiner
BACKGROUND: Cobalamin (vitamin B12 ) and folate (vitamin B9 ) are important for the amino acid metabolism and nucleic acid synthesis. Immunoassays for the measurement of both vitamins in serum are routinely used in people, cats, and dogs, serving as indicators for clinical disorders including cobalamin and/or folate deficiency, small intestinal dysbiosis, or inadequate dietary supply of these vitamins. The analysis of these analytes may also be clinically useful in pigs. OBJECTIVE: The purpose of this study was to analytically validate immunoassays for the measurement of cobalamin and folate concentrations in porcine serum, and to determine serum cobalamin and folate concentrations in healthy newborn pigs pre- and postweaning...
June 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27062346/a-prospective-placebo-controlled-pilot-evaluation-of-the-effect-of-omeprazole-on-serum-calcium-magnesium-cobalamin-gastrin-concentrations-and-bone-in-cats
#20
E Gould, C Clements, A Reed, L Giori, J M Steiner, J A Lidbury, J S Suchodolski, M Brand, T Moyers, L Emery, M K Tolbert
BACKGROUND: Chronic proton pump inhibitor administration has been associated with electrolyte and cobalamin deficiency, disrupted bone homeostasis, hypergastrinemia, and rebound acid hypersecretion in humans. It is unknown if this occurs in cats. OBJECTIVES: Prolonged oral omeprazole results in altered bone mineral density or content, serum calcium, magnesium, cobalamin, and gastrin concentrations in healthy cats. ANIMALS: Six healthy adult DSH cats...
May 2016: Journal of Veterinary Internal Medicine
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