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https://www.readbyqxmd.com/read/29735563/natamycin-and-azithromycin-are-synergistic-in-vitro-against-ocular-pathogenic-aspergillus-flavus-species-complex-and-fusarium-solani-species-complex-isolates
#1
Haoyi Guo, Lutan Zhou, Yi He, Chuanwen Gao, Lei Han, Yan Xu
The interaction of natamycin-azithromycin combination against 60 ocular fungal isolates was tested in vitro The combination produced 100% synergistic interactions when natamycin added azithromycin at 20, 40, 50 μg/ml against Aspergillus flavus species complex (AFSC) isolates and added azithromycin at 50 μg/ml against Fusarium solani species complex isolates. The combination with 50 μg/ml azithromycin enhanced natamycin's effect against AFSC isolates by reducing natamycin MICs from MIC90 64μg/ml to MIC90 0...
May 7, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29689547/sirt3-facilitates-amniotic-fluid-stem-cells-to-repair-diabetic-nephropathy-through-protecting-mitochondrial-homeostasis-by-modulation-of-mitophagy
#2
Jianxun Feng, Chang Lu, Qin Dai, Junqin Sheng, Min Xu
BACKGROUND/AIMS: Amniotic fluid stem cells (AFSCs) transplantation is a promising therapeutic strategy for diabetic nephropathy. Sirtuin3 (SIRT3) is a novel mitochondrial protective factor. In the present study, we aimed to investigate whether SIRT3 protects against hyperglycemia-induced AFSCs damage and enhances the therapeutic efficiency of AFSCs in diabetic nephropathy. METHODS: To establish the diabetic nephropathy model, db/ db mice were used. AFSCs were obtained and transplanted into the kidney tissue of db/ db mice...
April 19, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29649411/the-in-vitro-differentiation-of-gdnf-gene-engineered-amniotic-fluid-derived-stem-cells-into-renal-tubular-epithelial-like-cells
#3
Ying Lu, Zhuojun Wang, Lu Chen, Jia Wang, Shulin Li, Caixia Liu, Dong Sun
Amniotic fluid is an alternative source of stem cells, and human amniotic fluid-derived stem cells (AFSCs) obtained from a small amount of amniotic fluid collected during the second trimester represent a novel source for use in regenerative medicine. These AFSCs are characterized by lower diversity, a higher proliferation rate and a wider differentiation capability than adult mesenchymal stem cells (MSCs). AFSCs are selected based on the cell surface marker c-kit receptor (CD117) using immunomagnetic sorting...
March 15, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29502286/biological-characterization-and-pluripotent-identification-of-ovine-amniotic-fluid-stem-cells
#4
Wenhua Pei, Tengfei Lu, Kunfu Wang, Meng Ji, Shuang Zhang, Fenghao Chen, Lu Li, Xiangchen Li, Weijun Guan
Mesenchymal stem cells derived from amniotic fluid have become one of the most potential stem cell source for cell-based therapy for the reason they can be harvested at low cost and without ethical problems. Here, we obtained amniotic fluid stem cells (AFSCs) from ovine amniotic fluid and studied the expansion capacity, cell markers expression, karyotype, and multilineage differentiation ability. In our work, AFSCs were subcultured to passage 62. The cell markers, CD29, CD44, CD73 and OCT4 which analyzed by RT-PCR were positive; CD44, CD73, CD90, CD105, NANOG, OCT4 analyzed by immunofluorescence and flow cytometry were also positive...
March 3, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29482456/long-term-hematopoietic-engraftment-of-congenic-amniotic-fluid-stem-cells-after-in-utero-intraperitoneal-transplantation-to-immune-competent-mice
#5
Panicos Shangaris, Stavros P Loukogeorgakis, Michael P Blundell, Eleni Petra, Steven W Shaw, Durrgah L Ramachandra, Panagiotis Maghsoudlou, Luca Urbani, Adrian J Thrasher, Paolo De Coppi, Anna L David
Clinical success of in utero transplantation (IUT) using allogeneic hematopoietic stem cells (HSCs) has been limited to fetuses that lack an immune response to allogeneic cells due to severe immunological defects, and where transplanted genetically normal cells have a proliferative or survival advantage. Amniotic fluid (AF) is an autologous source of stem cells with hematopoietic potential that could be used to treat congenital blood disorders. We compared the ability of congenic and allogeneic mouse AF stem cells (AFSC) to engraft the hematopoietic system of time-mated C57BL/6J mice (E13...
April 15, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29348392/rapamycin-prevents-the-intervertebral-disc-degeneration-via-inhibiting-differentiation-and-senescence-of-annulus-fibrosus-cells
#6
Changhong Gao, Bin Ning, Chenglin Sang, Ying Zhang
The effects of bleomycin and rapamycin on cellular senescence and differentiation of rabbit annulus fibrosus stem cells (AFSCs) were investigated using a cell culture model. The results showed that bleomycin induced cellular senescence in AFSCs as evidenced by senescence-associated secretory phenotype. The morphology of AFSCs was changed from cobblestone-like cells to pancake-like cells. The senescence-associated β-galactosidase activity, the protein expression of P16 and P21, and inflammatory-related marker gene levels IL-1β, IL-6, and TNF-α were increased in bleomycin-treated AFSCs in a dose-dependent manner...
January 18, 2018: Aging
https://www.readbyqxmd.com/read/29341292/amniotic-fluid-stem-cell-exosomes-therapeutic-perspective
#7
Francesca Beretti, Manuela Zavatti, Francesca Casciaro, Giuseppina Comitini, Fabrizia Franchi, Veronica Barbieri, Giovanni B La Sala, Tullia Maraldi
It is widely accepted that the therapeutic potential of stem cells can be largely mediated by paracrine factors, also included into exosomes. Thus, stem cell-derived exosomes represent a major therapeutic option in regenerative medicine avoiding, if compared to stem cells graft, abnormal differentiation and tumor formation. Exosomes derived from mesenchymal stem cells (MSC) induce damaged tissue repair, and can also exert immunomodulatory effects on the differentiation, activation and function of different lymphocytes...
January 16, 2018: BioFactors
https://www.readbyqxmd.com/read/29239050/therapeutic-potential-of-amniotic-fluid-stem-cells-to-treat-bilateral-ovarian-dystrophy-in-dairy-cows-in-a-subtropical-region
#8
L-B Chang, S-Y Peng, C-J Chou, Y-J Chen, J-S Shiu, P-A Tu, S-X Gao, Y-C Chen, T-K Lin, S-C Wu
Amniotic fluid is a rich source of multipotent mesenchymal stem cells (MSCs). Amniotic fluid stem cells (AFSCs) have become a new source of stem cells; they have low immunogenicity and are easily harvested. For this reason, they may be useful in clinical tissue engineering. Moreover, AFSCs have anti-inflammatory properties and can repair tissues. This study evaluated the utility of AFSC injection to treat bilateral ovarian dystrophy in Holstein-Friesian cows. Bovine AFSCs (BAFSCs) were collected at slaughter from Holstein-Friesian cows during the third or fourth month of pregnancy and cultured in vitro...
April 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29233244/modeling-predictors-of-duties-not-including-flying-status
#9
Anthony P Tvaryanas, Converse Griffith
INTRODUCTION: The purpose of this study was to reuse available datasets to conduct an analysis of potential predictors of U.S. Air Force aircrew nonavailability in terms of being in "duties not to include flying" (DNIF) status. METHODS: This study was a retrospective cohort analysis of U.S. Air Force aircrew on active duty during the period from 2003-2012. Predictor variables included age, Air Force Specialty Code (AFSC), clinic location, diagnosis, gender, pay grade, and service component...
January 1, 2018: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/29203902/amniotic-fluid-stem-cell-derived-vesicles-protect-from-vegf-induced-endothelial-damage
#10
S Sedrakyan, V Villani, S Da Sacco, N Tripuraneni, S Porta, A Achena, M Lavarreda-Pearce, A Petrosyan, H Soloyan, R E De Filippo, B Bussolati, L Perin
Injection of amniotic fluid stem cells (AFSC) delays the course of progression of renal fibrosis in animals with Alport Syndrome, enhancing kidney function and improving survival. The mechanisms responsible for these protective outcomes are still largely unknown. Here, we showed that vascular endothelial growth factor (VEGF) signaling within the glomeruli of Alport mice is strongly elevated early on in the disease, causing glomerular endothelial cell damage. Intraventricular injected AFSC that homed within the glomeruli showed strong modulation of the VEGF activity, particularly in glomerular endothelial cells...
December 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29114949/protective-effect-of-gdnf-engineered-amniotic-fluid-derived-stem-cells-on-the-renal-ischaemia-reperfusion-injury-in-vitro
#11
Jia Wang, Fengzhen Wang, Zhuojun Wang, Shulin Li, Lu Chen, Caixia Liu, Dong Sun
OBJECTIVES: Amniotic fluid-derived stem cells (AFSCs) possessing multilineage differentiation potential are proposed as a novel and accessible source for cell-based therapy and tissue regeneration. Glial-derived neurotrophic factor (GDNF) has been hypothesized to promote the therapeutic effect of AFSCs on markedly ameliorating renal dysfunction. The aim of this study was to investigate whether AFSCs equipped with GDNF (GDNF-AFSCs) had capabilities of attenuating mouse renal tubular epithelial cells (mRTECs) apoptosis and evaluate its potential mechanisms...
November 7, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29056972/methotrexate-and-valproic-acid-affect-early-neurogenesis-of-human-amniotic-fluid-stem-cells-from-myelomeningocele
#12
Vardine Sahakyan, Enrico Pozzo, Robin Duelen, Jan Deprest, Maurilio Sampaolesi
Myelomeningocele (MMC) is a severe type of neural tube defect (NTD), in which the backbone and spinal canal do not close completely during early embryonic development. This condition results in serious morbidity and increased mortality after birth. Folic acid significantly reduces, and conversely, folate antagonist methotrexate (MTX) and valproic acid (VPA) increase the occurrence of NTDs, including MMC. How these pharmacological agents exactly influence the early neurulation process is still largely unclear...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28679310/protein-and-molecular-characterization-of-a-clinically-compliant-amniotic-fluid-stem-cell-derived-extracellular-vesicle-fraction-capable-of-accelerating-muscle-regeneration-through-enhancement-of-angiogenesis
#13
Ben Mellows, Robert Mitchell, Manuela Antonioli, Oliver Kretz, David Chambers, Marie-Theres Zeuner, Bernd Denecke, Luca Musante, Durrgah L Ramachandra, Florence Debacq-Chainiaux, Harry Holthofer, Barbara Joch, Steve Ray, Darius Widera, Anna L David, Tobias B Huber, Joern Dengjel, Paolo De Coppi, Ketan Patel
The secretome of human amniotic fluid stem cells (AFSCs) has great potential as a therapeutic agent in regenerative medicine. However, it must be produced in a clinically compliant manner before it can be used in humans. In this study, we developed a means of producing a biologically active secretome from AFSCs that is free of all exogenous molecules. We demonstrate that the full secretome is capable of promoting stem cell proliferation, migration, and protection of cells against senescence. Furthermore, it has significant anti-inflammatory properties...
September 15, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28640098/the-characteristics-of-stem-cells-in-human-degenerative-intervertebral-disc
#14
Lin Liang, Xuefeng Li, Dapeng Li, Weimin Jiang, Heng Wang, Jie Chen, Zhiyong Sun, Niannian Zhang, Yangyi Zhu
BACKGROUND: The aim of this study is to identify which possessed the best stem-cell-like characteristics in 3 kinds of cell in human degenerative intervertebral disc: NPSCs (nucleus pulposus-derived stem cells), AFSCs (annulus fibrosus-derived stem cells), or CESCs (cartilage endplate-derived stem cells). METHODS: We separated the disc samples obtained from 15 surgically treated patients with disc degenerative diseases into nucleus pulposus, annulus fibrosus, and cartilage endplate...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28545044/differentiation-of-spontaneously-contracting-cardiomyocytes-from-non-virally-reprogrammed-human-amniotic-fluid-stem-cells
#15
Aaron J Velasquez-Mao, Christopher J M Tsao, Madeline N Monroe, Xavier Legras, Beatrice Bissig-Choisat, Karl-Dimiter Bissig, Rodrigo Ruano, Jeffrey G Jacot
Congenital heart defects are the most common birth defect. The limiting factor in tissue engineering repair strategies is an autologous source of functional cardiomyocytes. Amniotic fluid contains an ideal cell source for prenatal harvest and use in correction of congenital heart defects. This study aims to investigate the potential of amniotic fluid-derived stem cells (AFSC) to undergo non-viral reprogramming into induced pluripotent stem cells (iPSC) followed by growth-factor-free differentiation into functional cardiomyocytes...
2017: PloS One
https://www.readbyqxmd.com/read/28104582/amniotic-fluid-derived-stem-cells-mixed-with-platelet-rich-plasma-for-restoration-of-rat-alveolar-bone-defect
#16
Minjiao Wang, Hongliang Li, Jiawen Si, Jiewen Dai, Jun Shi, Xudong Wang, Lihe Guo, Guofang Shen
Stem cells isolated from the amniotic fluid have been shown as a promising candidate for cell therapy and tissue engineering. However, the experimental and preclinical applications of amniotic fluid-derived stem cells (AFSCs) in the very field of maxillofacial bone tissue engineering are still limited. In this study, rat AFSCs were successfully harvested and characterized in vitro. The rat AFSCs showed typical fibroblastoid morphology, stable proliferation activity and multi-differentiation potential. Flow-cytometry analysis demonstrated that these cells were positive for CD29, CD44, and CD90, while negative for hematopoietic markers such as CD34 and CD45...
March 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27995994/counteracting-bone-fragility-with-human-amniotic-mesenchymal-stem-cells
#17
Anna M Ranzoni, Michelangelo Corcelli, Kwan-Leong Hau, Jemma G Kerns, Maximilien Vanleene, Sandra Shefelbine, Gemma N Jones, Dafni Moschidou, Benan Dala-Ali, Allen E Goodship, Paolo De Coppi, Timothy R Arnett, Pascale V Guillot
The impaired maturation of bone-forming osteoblasts results in reduced bone formation and subsequent bone weakening, which leads to a number of conditions such as osteogenesis imperfecta (OI). Transplantation of human fetal mesenchymal stem cells has been proposed as skeletal anabolic therapy to enhance bone formation, but the mechanisms underlying the contribution of the donor cells to bone health are poorly understood and require further elucidation. Here, we show that intraperitoneal injection of human amniotic mesenchymal stem cells (AFSCs) into a mouse model of OI (oim mice) reduced fracture susceptibility, increased bone strength, improved bone quality and micro-architecture, normalised bone remodelling and reduced TNFα and TGFβ sigalling...
December 20, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27873362/influence-of-mesenchymal-stem-cells-conditioned-media-on-proliferation-of-urinary-tract-cancer-cell-lines-and-their-sensitivity-to-ciprofloxacin
#18
Malgorzata Maj, Anna Bajek, Ewelina Nalejska, Dorota Porowinska, Tomasz Kloskowski, Lidia Gackowska, Tomasz Drewa
Mesenchymal stem cells (MSCs) are known to interact with cancer cells through direct cell-to-cell contact and secretion of paracrine factors, although their exact influence on tumor progression in vivo remains unclear. To better understand how fetal and adult stem cells affect tumors, we analyzed viability of human renal (786-0) and bladder (T24) carcinoma cell lines cultured in conditioned media harvested from amniotic fluid-derived stem cells (AFSCs) and adipose-derived stem cells (ASCs). Both media reduced metabolic activity of 786-0 cells, however, decreased viability of T24 cells was noted only after incubation with conditioned medium from ASCs...
June 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27703485/differential-characterization-of-two-kinds-of-stem-cells-isolated-from-rabbit-nucleus-pulposus-and-annulus-fibrosus
#19
Chenglin Sang, Xuecheng Cao, Fangjing Chen, Xuekang Yang, Yongxian Zhang
Objective. Nucleus pulposus (NP) and annulus fibrosus (AF) are two main components of intervertebral disc (IVD). We aimed to figure out whether NP and AF also contain stem cells and whether these stem cells share common properties with chondrocytes and/or fibroblasts in their phenotypes or whether they are completely different types of cells with different characteristics. Design. The disk cells were isolated from AF and NP tissues of the same lumbar spine of the rabbits. The properties of these disk cells were characterized by their morphology, population doubling time (PDT), stem cell marker expression, and multidifferentiation potential using tissue culture techniques, immunocytochemistry, and RT-PCR...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27684534/an-update-clinical-application-of-amniotic-fluid-derived-stem-cells-afscs-in-cancer-cell-therapy-and-tissue-engineering
#20
REVIEW
Shiva Gholizadeh-Ghaleh Aziz, Ezzatollah Fathi, Mohammad Rahmati-Yamchi, Abolfazl Akbarzadeh, Zahra Fardyazar, Maryam Pashaiasl
Recent studies have elucidated that cell-based therapies are promising for cancer treatments. The human amniotic fluid stem (AFS) cells are advantageous cells for such therapeutic schemes that can be innately changed to express therapeutic proteins. HAFSCs display a natural tropism to cancer cells in vivo. They can be useful in cancer cells targeting. Moreover, they are easily available from surplus diagnostic samples during pregnancy and less ethical and legal concern are associated with the collection and application than other putative cells are subjected...
June 2017: Artificial Cells, Nanomedicine, and Biotechnology
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