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https://www.readbyqxmd.com/read/29158549/hypoxia-inducible-factor-1%C3%AE-mediates-the-profibrotic-effect-of-albumin-in-renal-tubular-cells
#1
Junping Hu, Weili Wang, Fan Zhang, Pin-Lan Li, Krishna M Boini, Fan Yi, Ningjun Li
Proteinuria is closely associated with the progression of chronic kidney diseases (CKD) by producing renal tubulointerstitial fibrosis. Over-activation of hypoxia inducible factor (HIF)-1α has been implicated in the progression of CKD. The present study tested the hypothesis that HIF-1α mediates albumin-induced profibrotic effect in cultured renal proximal tubular cells. Incubation of the cells with albumin (40 μg/ml) for 72 hrs significantly increased the protein levels of HIF-1α, tissue inhibitor of metalloproteinase (TIMP)-1 and collagen-I, which were blocked by HIF-1α shRNA...
November 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29154223/mitochondrial-f1-atpase-extends-glycolysis-and-ph-decline-in-an-in-vitro-model
#2
Sulaiman K Matarneh, Mariane Beline, Saulo de Luz E Silva, Hao Shi, David E Gerrard
The experiment was conducted to identify the mitochondrial protein responsible for enhancing glycolytic flux. We hypothesized that mitochondrial F1-ATPase promotes ATP hydrolysis and thereby the flux through glycolysis. Porcine longissimus muscle mitochondria were incorporated into an in vitro system designed to recapitulate postmortem glycolysis with or without Na-azide to specifically inhibit the β-subunit of mitochondrial F1-ATPase that catalyzes ATP hydrolysis. Addition of mitochondria enhanced ATP hydrolysis, glycogen degradation, lactate accumulation, and pH decline in the in vitro system...
November 7, 2017: Meat Science
https://www.readbyqxmd.com/read/29138805/extracellular-polysaccharides-purified-from-aureobasidium-pullulans-sm%C3%A2-2001-polycan-inhibit-dexamethasone%C3%A2-induced-muscle-atrophy-in-mice
#3
Jong-Min Lim, Young Joon Lee, Hyung-Rae Cho, Dong-Chan Park, Go-Woon Jung, Sae Kwang Ku, Jae-Suk Choi
The present study assessed the beneficial skeletal muscle‑preserving effects of extracellular polysaccharides from Aureobasidium pullulans SM‑2001 (Polycan) (EAP) on dexamethasone (DEXA)‑induced catabolic muscle atrophy in mice. To investigate whether EAP prevented catabolic DEXA‑induced muscle atrophy, and to examine its mechanisms of action, EAP (100, 200 and 400 mg/kg) was administered orally, once a day for 24 days. EAP treatment was initiated 2 weeks prior to DEXA treatment (1 mg/kg, once a day for 10 days) in mice...
November 10, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29130633/the-mitochondrial-metabolic-reprogramming-agent-trimetazidine-as-an-exercise-mimetic-in-cachectic-c26-bearing-mice
#4
Francesca Molinari, Fabrizio Pin, Stefania Gorini, Sergio Chiandotto, Laura Pontecorvo, Fabio Penna, Emanuele Rizzuto, Simona Pisu, Antonio Musarò, Paola Costelli, Giuseppe Rosano, Elisabetta Ferraro
BACKGROUND: Cancer cachexia is characterized by muscle depletion and exercise intolerance caused by an imbalance between protein synthesis and degradation and by impaired myogenesis. Myofibre metabolic efficiency is crucial so as to assure optimal muscle function. Some drugs are able to reprogram cell metabolism and, in some cases, to enhance metabolic efficiency. Based on these premises, we chose to investigate the ability of the metabolic modulator trimetazidine (TMZ) to counteract skeletal muscle dysfunctions and wasting occurring in cancer cachexia...
November 11, 2017: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/29129636/inflammation-mediated-muscle-metabolic-dysregulation-local-and-remote-to-the-site-of-major-abdominal-surgery
#5
Krishna K Varadhan, Dumitru Constantin-Teodosiu, Despina Constantin, Paul L Greenhaff, Dileep N Lobo
BACKGROUND & AIMS: Postoperative hyperglycaemia is common in patients having major surgery and is associated with adverse outcomes. This study aimed to determine whether bacteraemia contributed to postoperative systemic inflammation, and whether increases in the expression of muscle mRNAs and proteins reflecting increased muscle inflammation, atrophy and impaired carbohydrate oxidation were evident at the time of surgery, and both local and distant to the site of trauma, and could be associated with impaired glucoregulation...
November 2, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/29123982/decreased-grip-strength-muscle-pain-and-atrophy-occur-in-rats-following-long-term-exposure-to-excessive-repetitive-motion
#6
Mitsuhiro Fujiwara, Masahiro Iwata, Takayuki Inoue, Yosuke Aizawa, Natsumi Yoshito, Kazuhiro Hayashi, Shigeyuki Suzuki
Work-related musculoskeletal disorders (WMSD) are caused by the overuse of muscles in the workplace. Performing repetitive tasks is a primary risk factor for the development of WMSD. Many workers in highly repetitive jobs exhibit muscle pain and decline in handgrip strength, yet the mechanisms underlying these dysfunctions are poorly understood. In our study, rats performed voluntary repetitive reaching and grasping tasks (Task group), while Control group rats did not perform these activities. In the Task group, grip strength and forearm flexor withdrawal threshold declined significantly from week 2 to week 6, compared with these values at week 0 (P < 0...
November 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/29121865/temporally-distinct-transcriptional-regulation-of-myocyte-dedifferentiation-and-myofiber-growth-during-muscle-regeneration
#7
Ke'ale W Louie, Alfonso Saera-Vila, Phillip E Kish, Justin A Colacino, Alon Kahana
BACKGROUND: Tissue regeneration requires a series of steps, beginning with generation of the necessary cell mass, followed by cell migration into damaged area, and ending with differentiation and integration with surrounding tissues. Temporal regulation of these steps lies at the heart of the regenerative process, yet its basis is not well understood. The ability of zebrafish to dedifferentiate mature "post-mitotic" myocytes into proliferating myoblasts that in turn regenerate lost muscle tissue provides an opportunity to probe the molecular mechanisms of regeneration...
November 9, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29121270/the-effect-of-atp-marination-on-the-depolymerization-of-actin-filament-in-goose-muscles-during-postmortem-conditioning
#8
Chang-Yu Zhou, Ying Wang, Dao-Dong Pan, Yang-Ying Sun, Jin-Xuan Cao
In order to study the tenderization mechanism of ATP treatments by depolymerizing actin filaments, breast muscles of Eastern Zhejiang White Geese were randomly divided into 3 groups: control, 10 and 20 mM groups. Shear force (SF), sarcomere length (SL) and myofibrillar fraction index (MFI), the content of F-actin and G-actin, the expression of actin associated proteins (cofilins and tropomodulins) were investigated during conditioning. In 20 mM group, cofilins content increased from 48 to 168 h, while tropomodulins decreased; the content of F-actin decreased from 24 to 168 h, while the increased G-actin was observed upto 48 h...
November 7, 2017: Poultry Science
https://www.readbyqxmd.com/read/29120776/comparative-proteomic-profiling-of-myofibrillar-proteins-in-dry-cured-ham-with-different-proteolysis-indices-and-adhesiveness
#9
M López-Pedrouso, C Pérez-Santaescolástica, D Franco, E Fulladosa, J Carballo, C Zapata, J M Lorenzo
Excessive proteolysis during dry-cured ham processing may lead to high adhesiveness and consumer dissatisfaction. The aim of this research is to identify biomarkers for proteolysis and adhesiveness. Two hundred biceps femoris porcine muscle samples from Spanish dry-cured ham were firstly evaluated for various physicochemical parameters, including their proteolysis indices and instrumental adhesiveness. Proteins of samples with extreme proteolysis indices were separated by two-dimensional electrophoresis and identified by tandem mass spectrometry (MALDI-TOF/TOF)...
April 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29112633/2017-military-supplement-current-challenges-in-the-development-of-acellular-hemoglobin-oxygen-carriers-by-protein-engineering
#10
Andres S Benitez Cardenas, Premila P Samuel, John S Olson
This article reviews the key biochemical mechanisms that govern O2 transport, NO scavenging, and oxidative degradation of acellular hemoglobin and how these ideas have been used to try to develop strategies to engineer safer and more effective hemoglobin-based oxygen carriers (HBOCs). Significant toxicities due to acellular hemoglobin (Hb) have been observed after the administration of HBOCs or after the lysis of red cells, and include: (a) rapid clearance and kidney damage due to dissociation into dimers, haptoglobin binding, and macrophage activation; (b) early O2 release leading to decreased tissue perfusion in capillary beds; (c) interference with endothelial and smooth muscle signaling due to nitric oxide (NO) scavenging; (d) autooxidization of heme iron followed by production of reactive oxygen species; and (e) iron overload symptoms due to hemin loss, globin denaturation, iron accumulation, and further inflammation...
November 6, 2017: Shock
https://www.readbyqxmd.com/read/29109401/smad3-initiates-oxidative-stress-and-proteolysis-that-underlies-diaphragm-dysfunction-during-mechanical-ventilation
#11
Huibin Tang, Catherine L Kennedy, Myung Lee, Yang Gao, Hui Xia, Francesca Olguin, Danielle A Fraga, Kelsey Ayers, Sehoon Choi, Michael Kim, Amir Tehrani, Yasser A Sowb, Thomas A Rando, Joseph B Shrager
Prolonged use of mechanical ventilation (MV) leads to atrophy and dysfunction of the major inspiratory muscle, the diaphragm, contributing to ventilator dependence. Numerous studies have shown that proteolysis and oxidative stress are among the major effectors of ventilator-induced diaphragm muscle dysfunction (VIDD), but the upstream initiator(s) of this process remain to be elucidated. We report here that periodic diaphragm contraction via phrenic nerve stimulation (PNS) substantially reduces MV-induced proteolytic activity and oxidative stress in the diaphragm...
November 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29107647/muscle-protective-effects-of-schisandrae-fructus-extracts-in-old-mice-after-chronic-forced-exercise
#12
Ki-Young Kim, Sae-Kwang Ku, Ki-Won Lee, Chang-Hyun Song, Won G An
ETHNOPHARMACOLOGICAL RELEVANCE: Schisandrae Fructus (SF), the dried fruit of Schisandra chinensis (Turcz.) Baill., is a well-known traditional herb used in Asia for enhancing physical work capacity as well as providing anti-stress and anti-inflammatory effects. Extracts of SF (SFe) have also been reported to increase skeletal muscle mass and inhibit muscle atrophy. AIM OF THE STUDY: We examined whether SFe had muscle-protective effects in old mice after chronic forced exercises, and, if so, relevant mechanisms...
October 28, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29107178/1-25-dihydroxycholecalciferol-calcitriol-modifies-uptake-and-release-of-25-hydroxycholecalciferol-in-skeletal-muscle-cells-in-culture
#13
M Abboud, M S Rybchyn, Y J Ning, T C Brennan-Speranza, C M Girgis, J E Gunton, D R Fraser, R S Mason
The major circulating metabolite of vitamin D3, 25-hydroxycholecalciferol [25(OH)D], has a remarkably long half-life in blood for a (seco)steroid. Data from our studies and others are consistent with the hypothesis that there is a role for skeletal muscle in the maintenance of vitamin D status. Muscle cells internalise vitamin D-binding protein (DBP) from the circulation by means of a megalin/cubilin plasma membrane transport mechanism. The internalised DBP molecules then bind to actin and thus provide an intracellular array of high affinity binding sites for its specific ligand, 25(OH)D...
October 26, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29104500/clenbuterol-induces-cell-cycle-arrest-in-c2c12-myoblasts-by-delaying-p27-degradation-through-%C3%AE-arrestin-2-signaling
#14
Min Chen, Chuncheng Liu, Meng Wang, Hong Wang, Kuo Zhang, Yu Zheng, Zhengquan Yu, Xiangdong Li, Wei Guo, Ning Li, Qingyong Meng
β2-Adrenoceptor (β2-AR) agonists promote muscle growth. The aim of this study was to elucidate some effects of the selective β2-adrenoceptor agonist clenbuterol (CLB) on myoblast proliferation. We found that CLB induces cell cycle arrest in C2C12 myoblasts. This effect is partly due to the enhanced stability of p27, rather than the increased gene transcription via cAMP response element-binding protein (CREB). Specifically, CLB treatment enhanced the accumulation of p27 in the nucleus while depleting it from the cytosol via a mechanism that requires β2-AR...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/29090065/cloning-expression-and-purification-of-pseudomonas-putida-atcc12633-creatinase
#15
Elnaz Afshari, Zahra Amini-Bayat, Saman Hosseinkhani, Nahid Bakhtiari
BACKGROUND: Pseudomonas putida (P. putida) ATCC12633 can produce creatinase. It is a microbial enzyme which degrades creatinine in bacteria and provides source of carbon and nitrogen. Also, this enzyme is used in the enzymatic measurement of creatinine concentration for diagnosis of renal and muscles functions and diseases. Our purpose was recombinant production of creatinase for using in clinical measurement of serum or urine creatinine. METHODS: A 1209bp of open reading frame of creatinase was amplified by PCR from P...
October 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/29082572/influence-of-stewing-time-on-the-texture-ultrastructure-and-in%C3%A2-vitro-digestibility-of-meat-from-the-yellow-feathered-chicken-breed
#16
Jun Qi, Xiao Li, Wenwen Zhang, Huhu Wang, Guanghong Zhou, Xinglian Xu
This study assessed the influence of stewing (1, 2 or 3 h) on the texture, ultrastructure and in vitro digestibility of meat from the yellow-feathered chicken, which is a popular broiler chicken in Asia. Results indicated that longer stewing considerably increased cooking loss of the chicken carcass and tenderness of thigh meat. After 3 h of stewing, protein digestibility decreased from 90.5% to 80.3% and fiber diameter decreased by 8.63 μm. The shear force value of breast meat decreased from 32.34 N at 1 h to 10...
October 30, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/29074597/s-nitrosoglutathione-reductase-dysfunction-contributes-to-obesity-associated-hepatic-insulin-resistance-via-regulating-autophagy
#17
Qingwen Qian, Zeyuan Zhang, Allyson Orwig, Songhai Chen, Wen-Xing Ding, Yanji Xu, Ryan C Kunz, Nicholas R L Lind, Jonathan S Stamler, Ling Yang
Obesity is associated with elevated intracellular nitric oxide (NO) production, which promotes nitrosative stress in metabolic tissues such as liver and skeletal muscle, contributing to insulin resistance. The onset of obesity-associated insulin resistance is due, in part, to the compromise of hepatic autophagy, a process that leads to lysosomal degradation of cellular components. However, it is not known how NO bioactivity might impact autophagy in obesity. Here, we establish that S-nitrosoglutathione reductase (GSNOR), a major protein denitrosylase, provides a key regulatory link between inflammation and autophagy, which is disrupted in obesity and diabetes...
October 26, 2017: Diabetes
https://www.readbyqxmd.com/read/29069832/acylated-and-unacylated-ghrelin-inhibit-atrophy-in-myotubes-co-cultured-with-colon-carcinoma-cells
#18
Xianliang Zeng, Sizeng Chen, Yang Yang, Zhao Ke
Cancer cachexia is a result of increased protein degradation and decreased protein synthesis. The multifunctional circulating hormone ghrelin promotes synthesis and inhibits degradation of muscle protein, but its mechanism of action is not fully understood. Here, we investigated whether co-culturing C2C12 myotubes with CT26 colon carcinoma cells induces myotube atrophy, and whether acylated ghrelin (AG) and unacylated ghrelin (UnAG) had anti-atrophic effects. We found that co-culture induced myotube atrophy and increased tumor necrosis factor-alpha (TNF-α) and myostatin concentrations in the culture medium...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29069356/glucocorticoid-receptor-signaling-impairs-protein-turnover-regulation-in-hypoxia-induced-muscle-atrophy-in-male-mice
#19
Chiel C de Theije, Annemie M W J Schols, Wouter H Lamers, Judith J M Ceelen, Rick H van Gorp, J J Rob Hermans, S Elonore Köhler, Ramon C J Langen
Hypoxemia may contribute to muscle wasting in conditions such as Chronic Obstructive Pulmonary Disease. Muscle wasting develops when muscle proteolysis exceeds protein synthesis. Hypoxia induces skeletal muscle atrophy in mice, which can in part be attributed to reduced food intake. We hypothesized that hypoxia elevates circulating corticosterone concentrations by reduced food intake and enhances GR signaling in muscle, which causes elevated protein degradation signaling and dysregulates protein synthesis signaling during hypoxia-induced muscle atrophy...
October 23, 2017: Endocrinology
https://www.readbyqxmd.com/read/29069231/myostatin-promotes-distinct-responses-on-protein-metabolism-of-skeletal-and-cardiac-muscle-fibers-of-rodents
#20
L H Manfredi, S Paula-Gomes, N M Zanon, I C Kettelhut
Myostatin is a novel negative regulator of skeletal muscle mass. Myostatin expression is also found in heart in a much less extent, but it can be upregulated in pathological conditions, such as heart failure. Myostatin may be involved in inhibiting protein synthesis and/or increasing protein degradation in skeletal and cardiac muscles. Herein, we used cell cultures and isolated muscles from rats to determine protein degradation and synthesis. Muscles incubated with myostatin exhibited an increase in proteolysis with an increase of Atrogin-1, MuRF1 and LC3 genes...
October 19, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
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