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cytometry part b

Pooja Sadana, Rebecca Geyer, Joern Pezoldt, Saskia Helmsing, Jochen Huehn, Michael Hust, Petra Dersch, Andrea Scrima
Yersinia pseudotuberculosis is a Gram-negative bacterium and zoonotic pathogen responsible for a wide range of diseases, ranging from mild diarrhea, enterocolitis, lymphatic adenitis to persistent local inflammation. The Y. pseudotuberculosis invasin D (InvD) molecule belongs to the invasin (InvA)-type autotransporter proteins, but its structure and function remain unknown. In this study, we present the first crystal structure of InvD, analyzed its expression and function in a murine infection model, and identified its target molecule in the host...
March 13, 2018: Journal of Biological Chemistry
Jacopo Agagliate, Ina Lefering, David McKee
A Mie-based forward modeling procedure was developed to reconstruct bulk inherent optical properties (IOPs) from particle size distributions (PSDs) and real refractive index distributions (PRIDs) obtained using a previously developed flow cytometric (FC) method [Appl. Opt.57, 1705 (2018)APOPAI0003-693510.1364/AO.57.001705]. Given the available PSDs, extrapolations for the particle fraction outside the detection limits of the method and a complex refractive index input (with real part nr directly estimated and imaginary part ni adapted from the literature separately for organic and inorganic components), the model produces volume scattering functions that are integrated to produce scattering and backscattering coefficients, and absorption efficiencies that are used to calculate absorption coefficients...
March 10, 2018: Applied Optics
Martin Müller, Ruth Seidenberg, Sabine K Schuh, Aristomenis K Exadaktylos, Clyde B Schechter, Alexander B Leichtle, Wolf E Hautz
OBJECTIVE: Patients presenting with suspected urinary tract infection are common in every day emergency practice. Urine flow cytometry has replaced microscopic urine evaluation in many emergency departments, but interpretation of the results remains challenging. The aim of this study was to develop and validate tools that predict urine culture growth out of urine flow cytometry parameter. METHODS: This retrospective study included all adult patients that presented in a large emergency department between January and July 2017 with a suspected urinary tract infection and had a urine flow cytometry as well as a urine culture obtained...
2018: PloS One
Dionysios C Watson, Eirini Moysi, Antonio Valentin, Cristina Bergamaschi, Santhi Devasundaram, Sotirios P Fortis, Jenifer Bear, Elena Chertova, Julian Bess, Ray Sowder, David J Venzon, Claire Deleage, Jacob D Estes, Jeffrey D Lifson, Constantinos Petrovas, Barbara K Felber, George N Pavlakis
B cell follicles in secondary lymphoid tissues represent an immune privileged sanctuary for AIDS viruses, in part because cytotoxic CD8+ T cells are mostly excluded from entering the follicles that harbor infected T follicular helper (TFH) cells. We studied the effects of native heterodimeric IL-15 (hetIL-15) treatment on uninfected rhesus macaques and on macaques that had spontaneously controlled SHIV infection to low levels of chronic viremia. hetIL-15 increased effector CD8+ T lymphocytes with high granzyme B content in blood, mucosal sites and lymph nodes, including virus-specific MHC-peptide tetramer+ CD8+ cells in LN...
February 2018: PLoS Pathogens
Andreas Ziebart, Ulrich Huber, Sandra Jeske, Simon Laban, Johannes Doescher, Thomas K Hoffmann, Cornelia Brunner, Edwin K Jackson, Patrick J Schuler
Introduction: Head and neck squamous cell carcinoma (HNSCC) strongly suppresses the immune system, resulting in increased metastasis and recurrent disease. Chemotherapy is part of the multimodal treatment but may further immunosuppression. Recently, we demonstrated that regulatory B cells (Breg), defined as CD19+ CD39+ CD73+ B cells, play a significant role in the production of immunosuppressive, extracellular adenosine (ADO). Here, we tested the influence of chemotherapy on Breg function...
January 19, 2018: Oncotarget
Darya Y Orlova, Stephen Meehan, David Parks, Wayne A Moore, Connor Meehan, Qian Zhao, Eliver E B Ghosn, Leonore A Herzenberg, Guenther Walther
Part of the flow/mass cytometry data analysis process is aligning (matching) cell subsets between relevant samples. Current methods address this cluster-matching problem in ways that are either computationally expensive, affected by the curse of dimensionality, or fail when population patterns significantly vary between samples. Here, we introduce a quadratic form (QF)-based cluster matching algorithm (QFMatch) that is computationally efficient and accommodates cases where population locations differ significantly (or even disappear or appear) from sample to sample...
February 19, 2018: Scientific Reports
Young Kim, William A Abplanalp, Andrew D Jung, Rebecca M Schuster, Alex B Lentsch, Erich Gulbins, Charles C Caldwell, Timothy A Pritts
Microparticles are submicron vesicles shed from aging erythrocytes as a characteristic feature of the red blood cell (RBC) storage lesion. Exposure of pulmonary endothelial cells to RBC-derived microparticles promotes an inflammatory response, but the mechanisms underlying microparticle-induced endothelial cell activation are poorly understood. In the present study, cultured murine lung endothelial cells (MLECs) were treated with microparticles isolated from aged murine packed RBCs or vehicle. Microparticle-treated cells demonstrated increased expression of the adhesion molecules ICAM and E-selectin, as well as the cytokine, IL-6...
March 2018: Shock
Yin Jia, Qin Qin, Chao-Ping Fang, Wei Shen, Ting-Ting Sun, Yuan-Lan Huang, Wen-Jie Li, An-Mei Deng
The aim of the current study was to understand the mechanisms of apoptosis occurring in cultured human lens epithelial cells (HLECs) following ultraviolet B (UVB) irradiation. The investigations intended to confirm the presence of apoptosis and to reveal the roles of oxidative stress, calcium (Ca2+), c‑Jun NH2‑terminal kinase (JNK)1/2, and extracellular signal‑regulated kinase (ERK)1/2 signaling pathway in these progresses. Cell apoptosis, ROS generation and intracellular Ca2+ concentration was measured by flow cytometry...
February 9, 2018: International Journal of Molecular Medicine
Yan Cai, Jiajing Cai, Qiang Ma, Yuan Xu, Jiang Zou, Lei Xu, Dongsheng Wang, Xiaolan Guo
Esophageal carcinoma is a malignancy that severely threatens human health, with a high incidence rate and a low 5-year survival rate. Resistance to chemotherapy frequently emerges during its treatment, partly due to the induction of autophagy. Therefore, targeting autophagy may be a promising therapeutic approach for the treatment of esophageal carcinoma. In the present study, it was investigated how chloroquine (CQ) can influence the growth ability and biological behaviors of EC109 esophageal squamous carcinoma cells in vitro, as well as the potential molecular mechanisms behind its activity...
January 2018: Oncology Letters
Aya Miyagawa-Hayashino, Hajime Yoshifuji, Koji Kitagori, Shinji Ito, Takuma Oku, Yoshitaka Hirayama, Adeeb Salah, Toshiki Nakajima, Kaori Kiso, Norishige Yamada, Hironori Haga, Tatsuaki Tsuruyama
BACKGROUND: Systemic lupus erythematosus (SLE) is a prototypical autoimmune disease in which dysregulation of B cells has been recognized. Here, we searched for potential biomarkers of SLE using liquid chromatography-tandem mass spectrometry (LC-MS). METHODS: Lymph nodes from SLE patients and controls were analyzed by LC-MS. To validate the identified molecules, immunoblotting and immunohistochemistry were performed and B cells from SLE patients were analyzed by quantitative RT-PCR...
January 30, 2018: Arthritis Research & Therapy
I Lajdova, A Oksa, M Horvathova, V Spustova
Chronic kidney disease (CKD) is known as a state of chronic low-grade inflammation, enhancing cardiovascular risk and immunodeficiency. Purinergic signaling has been accepted as a crucial component in the pathogenesis of various diseases, mediating a vast array of biological processes. The P2X7 receptor is one of the important cell surface regulators of several key inflammatory molecules. The aim of the study was to examine the expression of surface P2X7 receptors in subpopulations of peripheral blood mononuclear cells (PBMCs), and to evaluate the promising prognostic markers of inflammation (neutrophil/lymphocyte, Ne/Ly ratio) and cardiovascular risk (monocyte/high density lipoprotein cholesterol, Mo/HDL ratio) in early-stage CKD...
October 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
Min Qiu, Huanli Sun, Fenghua Meng, Ru Cheng, Jian Zhang, Chao Deng, Zhiyuan Zhong
Doxil® is the first FDA-approved anti-cancer nano-drug. Notably, no targeted liposomal formulation has advanced to clinical stage despite tremendous work undertaken, partly due to a low stability of liposomes. Here, we report on novel lipopepsomes self-assembled from poly(ethylene glycol)-b-poly(α-aminopalmitic acid) as a stable and versatile alternative to liposomes for highly efficient encapsulation and tumor-targeted delivery of doxorubicin hydrochloride (Dox·HCl). Interestingly, lipopepsomes could be easily decorated with 20mol% cRGD peptide and loaded with 17...
January 17, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
Raquel A Pinna, Adriana C Dos Santos, Daiana S Perce-da-Silva, Luciene A da Silva, Rodrigo N R da Silva, Marcelo R Alves, Fátima Santos, Joseli de Oliveira Ferreira, Josué C Lima-Junior, Déa M Villa-Verde, Paula M De Luca, Carla E Carvalho-Pinto, Dalma M Banic
INTRODUCTION: A proliferation-inducing ligand (APRIL) and B cell activation factor (BAFF) are known to play a significant role in the pathogenesis of several diseases, including BAFF in malaria. The aim of this study was to investigate whether APRIL and BAFF plasma concentrations could be part of inflammatory responses associated with P. vivax and P. falciparum malaria in patients from the Brazilian Amazon. METHODS: Blood samples were obtained from P. vivax and P...
January 3, 2018: Immunity, Inflammation and Disease
Keita Sakaguchi, Akemi Koyanagi, Fumitaka Kamachi, Akiko Harauma, Asako Chiba, Ken Hisata, Toru Moriguchi, Toshiaki Shimizu, Sachiko Miyake
BACKGROUND: Breast milk contains important nutrients and immunoregulatory factors that are essential for newborn infants. Recently, epidemiological studies suggested that breastfeeding prevents a wide range of infectious diseases and lowers the incidence of infant allergic diseases. METHODS: To examine the effects of breast milk in immunological development in infancy, we established an artificial rearing system for hand-feeding mice and compared mouse pups fed with either breast milk or milk substitute...
December 31, 2017: Pediatrics International: Official Journal of the Japan Pediatric Society
Bo-Fang Zhang, Hong Jiang, Jing Chen, Xin Guo, Qi Hu, Shuo Yang
Previous studies have indicated that lysine (K)‑specific demethylase 3A (KDM3A) is associated with diverse diabetes‑associated cardiovascular complications in response to high glucose levels. However, the effects of KDM3A on the pathological progression of cardiovascular injuries in response to high insulin levels remain unknown. The present study aimed to explore whether KDM3A knockdown may attenuate high insulin‑induced vascular smooth muscle cell (VSMC) dysfunction, and to further investigate the underlying mechanisms...
December 29, 2017: International Journal of Molecular Medicine
Ngar-Woon Kam, Dehua Liu, Zhe Cai, Wah-Yan Mak, Chun-Kwok Wong, Kwok-Hing Chiu, Kam-Yiu Wong, Wai-Leuk Tsang, Lai-Shan Tam
OBJECTIVE: Elevated expression of interleukin 35 (IL-35) is associated with autoimmune disease, including rheumatoid arthritis (RA). The present study was undertaken to determine the functional interaction among IL-35, B cells, and stromal cells residing in the synovium of patients with RA and osteoarthritis (OA). METHODS: IL-35 (EBI-3/p35) expression was investigated in RA and OA synovium using quantitative real-time PCR (qRT-PCR) and immunohistochemistry. IL-35 receptor (IL-35R) expression on B cells dissociated from synovium and periphery of patients with RA, OA, and healthy donor controls (HC) was determined by flow cytometry...
December 15, 2017: Journal of Rheumatology
Pranav Tiwari, Sakshi Agarwal, Sachchidanand Srivastava, Shilpee Jain
Thermal therapy combined with chemotherapy is one of the advanced and efficient methods to eradicate cancer. In this work, we fabricated magnetically actuated smart textured (MAST) fibrous systems and studied their candidacy for cancer treatment. The polycaprolactone-Fe3 O4 based MAST fibers were fabricated using electrospinning technique. These MAST fibrous systems contained carbogenic quantum dots as a tracking agent and doxorubicin hydrochloride anticancer drug. Additionally, as fabricated MAST fibrous systems were able to deliver anticancer drug and heat energy simultaneously to kill HeLa cells in a 10 min period in vitro...
January 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Wen-Wei Zou, Su-Ping Xu
Retinoblastoma is reported as a rare cancer that occurs during childhood. Although several treatments are available for retinoblastoma, there is a need for alternative new treatment modalities for retinoblastoma with better safety and efficacy profile. Galangin (3,5,7-trihydroxyflavone), is a flavonoid compound, which is found in high concentration in lesser galangal. Galangin has been reported to have various bioactivities, including anti-inflammation, anti-oxidative stress and anti-cancer through various pathways...
November 6, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Meredith C Frie, Kelly R B Sporer, Brian W Kirkpatrick, Paul M Coussens
Johne's disease (JD) is a chronic wasting disease of ruminants caused by infection with Mycobacterium avium subspecies paratuberculosis (MAP). JD is particularly problematic on US dairy farms: estimates show that over 50% of farms are MAP-contaminated and as many as 91% of dairy herds could be infected. Although estimates vary widely, JD may cost the dairy industry between $200 million and $1.5 billion every year. One major obstacle to JD management is that JD is difficult to detect in many animals, in part due to the variable immunity against MAP demonstrated by JD+ cattle...
December 2017: Veterinary Immunology and Immunopathology
Lorise C Gahring, Elizabeth J Myers, Diane M Dunn, Robert B Weiss, Scott W Rogers
Cigarette smoking (CS) is a principal contributor to a spectrum of devastating lung diseases whose occurrence and severity may vary between individuals and not appear for decades after prolonged use. One explanation for the variability and delay in disease onset is that nicotine, the addictive component of CS, acts through the ionotropic nicotinic acetylcholine receptor (nAChR) alpha7 (α7) to modulate anti-inflammatory protection. In this study we measured the impact α7 signaling has on the mouse distal lung response to side-stream CS exposure for mice of the control genotype (α7G) and those in which the α7-receptor signaling mechanisms are restricted by point mutation (α7E260A:G)...
2017: PloS One
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