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cytometry part b

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https://www.readbyqxmd.com/read/29136761/galangin-inhibits-the-cell-progression-and-induces-cell-apoptosis-through-activating-pten-and-caspase-3-pathways-in-retinoblastoma
#1
Wen-Wei Zou, Su-Ping Xu
Retinoblastoma is reported as a rare cancer that occurs during childhood. Although several treatments are available for retinoblastoma, there is a need for alternative new treatment modalities for retinoblastoma with better safety and efficacy profile. Galangin (3,5,7-trihydroxyflavone), is a flavonoid compound, which is found in high concentration in lesser galangal. Galangin has been reported to have various bioactivities, including anti-inflammation, anti-oxidative stress and anti-cancer through various pathways...
November 6, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29129227/t-and-b-cell-activation-profiles-from-cows-with-and-without-johne-s-disease-in-response-to-in-vitro-stimulation-with-mycobacterium-avium-subspecies-paratuberculosis
#2
Meredith C Frie, Kelly R B Sporer, Brian W Kirkpatrick, Paul M Coussens
Johne's disease (JD) is a chronic wasting disease of ruminants caused by infection with Mycobacterium avium subspecies paratuberculosis (MAP). JD is particularly problematic on US dairy farms: estimates show that over 50% of farms are MAP-contaminated and as many as 91% of dairy herds could be infected. Although estimates vary widely, JD may cost the dairy industry between $200 million and $1.5 billion every year. One major obstacle to JD management is that JD is difficult to detect in many animals, in part due to the variable immunity against MAP demonstrated by JD+ cattle...
December 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29117258/lung-epithelial-response-to-cigarette-smoke-and-modulation-by-the-nicotinic-alpha-7-receptor
#3
Lorise C Gahring, Elizabeth J Myers, Diane M Dunn, Robert B Weiss, Scott W Rogers
Cigarette smoking (CS) is a principal contributor to a spectrum of devastating lung diseases whose occurrence and severity may vary between individuals and not appear for decades after prolonged use. One explanation for the variability and delay in disease onset is that nicotine, the addictive component of CS, acts through the ionotropic nicotinic acetylcholine receptor (nAChR) alpha7 (α7) to modulate anti-inflammatory protection. In this study we measured the impact α7 signaling has on the mouse distal lung response to side-stream CS exposure for mice of the control genotype (α7G) and those in which the α7-receptor signaling mechanisms are restricted by point mutation (α7E260A:G)...
2017: PloS One
https://www.readbyqxmd.com/read/29115442/golph3-expression-promotes-the-resistance-of-ht29-cells-to-5%C3%A2-fluorouracil-by-activating-multiple-signaling-pathways
#4
Ming-Zhen Wang, Cheng-Zhi Qiu, Wai-Shi Yu, Yan-Ta Guo, Chun-Xiao Wang, Zhi-Xiong Chen
The novel proto‑oncogene Golgi phosphoprotein (GOLPH)3 is overexpressed in a variety of tumor tissues and is associated with poor prognosis. The authors previously demonstrated that GOLPH3 gene is overexpressed in colorectal cancer tissues and promotes the proliferation of colonic cancer cells by activating the phosphatidylinositol‑3‑kinase/protein kinase B/the mammalian target of rapamycin and Wnt/β‑catenin signaling pathways. However, to the best of the authors' knowledge, if and how the GOLPH3 gene is involved in inducing resistance to colonic cancer chemotherapy has not been reported...
October 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29113227/low-dose-valproic-acid-with-low-dose-gemcitabine-augments-mhc-class-i-related-chain-a-b-expression-without-inducing-the-release-of-soluble-mhc-class-i-related-chain-a-b
#5
Tomoharu Miyashita, Kenji Miki, Takashi Kamigaki, Isamu Makino, Hidehiro Tajima, Shinichi Nakanuma, Hironori Hayashi, Hiroyuki Takamura, Sachio Fushida, Ali K Ahmed, John W Harmon, Tetsuo Ohta
To improve natural killer group 2 member D (NKG2D)-dependent cytotoxicity, the inhibition of cleavage and release of major histocompatibility complex class 1-related chain (MIC) molecules from the tumor surface are required. Valproic acid (VPA), a histone deacetylase (HDAC) inhibitor, is able to induce cell-surface MICA/B on tumor cells. In the present study, the ability of VPA and gemcitabine (GEM) to upregulate MICA/B in pancreatic cancer cells was investigated, resulting in the inhibition of cleavage and release of MIC molecules from the tumor surface...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29108912/autophagy-activation-is-required-for-influenza-a-virus-induced-apoptosis-and-replication
#6
B Yeganeh, S Ghavami, Md N Rahim, T Klonisch, A J Halayko, K M Coombs
Autophagy and apoptosis are two major interconnected host cell responses to viral infection, including influenza A virus (IAV). Thus, delineating these events could facilitate the development of better treatment options and provide an effective anti-viral strategy for controlling IAV infection. We used A549 cells and mouse embryonic fibroblasts (MEF) to study the role of virus-induced autophagy and apoptosis, the cross-talk between both pathways, and their relation to IAV infection [ATCC strain A/Puerto Rico/8/34(H1N1) (hereafter; PR8)]...
November 3, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29098021/pyrrolidine-dithiocarbamate-sensitizes-u251-brain-glioma-cells-to-temozolomide-via-downregulation-of-mgmt-and-bcl-xl
#7
Jun-Hai Tang, Guo-Hao Huang, Ke-Jie Mou, Eric Erquan Zhang, Ningning Li, Lei Du, Xiao-Peng Zhu, Ling Chen, Hui Yang, Ke-Bin Zhang, Sheng-Qing Lv
The current study investigated the effect of pyrrolidine dithiocarbamate (PDTC) on the proliferation, apoptosis, cell cycle and sensitivity to temozolomide (TMZ) of the U251 glioma cell line. Proliferation, apoptosis and cell cycle analysis of U251 cells following treatment with PDTC and TMZ was determined by an MTT assay and flow cytometry, respectively. The mRNA and protein expression levels of O-6-methylguanine-DNA methyltransferase (MGMT), B-cell lymphoma extra-large (BCL-XL) and survivin were further determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting analysis...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29050153/-study-on-the-differentiation-of-rat-bone-marrow-mesenchymal-stem-cells-into-chondrocytes-induced-by-tgf-beta-3
#8
D B Liu, X S Han, W L Huang, J Deng, R F She
Objective: To explore the methods for the differentiation of rat bone marrow mesenchymal stem cells (BMMSCs) into chondrocytes induced by transforming growth factor (TGF)-beta 3 in vitro, and search for a reliable seed cell for the regeneration and repair of articular cartilage in osteoarthritis. Methods: SD rat femoral and tibial BMMSCs were cultured by the whole bone marrow adherent method, and then the purity was identified by flow cytometry. P3 generation cells were induced and differentiated into chondrocytes by the induction of differentiation medium containing TGF-beta 3, and cell chondrogenic differentiation ability at different induction time points was detected...
September 26, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29027719/glioplasticity-in-irritable-bowel-syndrome
#9
N L Lilli, L Quénéhervé, S Haddara, C Brochard, P Aubert, M Rolli-Derkinderen, T Durand, P Naveilhan, J-B Hardouin, R De Giorgio, G Barbara, S Bruley des Varannes, E Coron, M Neunlist
BACKGROUND: Growing evidence indicates a wide array of cellular remodeling in the mucosal microenvironment during irritable bowel syndrome (IBS), which possibly contributes to pathophysiology and symptom generation. Here, we investigated whether enteric glial cells (EGC) may be altered, and which factors/mechanisms lead to these changes. METHODS: Colonic mucosal biopsies of IBS patients (13 IBS-Constipation [IBS-C]; 10 IBS-Diarrhea [IBS-D]; 11 IBS-Mixed [IBS-M]) and 24 healthy controls (HC) were analyzed...
October 13, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/29023707/guidelines-for-the-use-of-flow-cytometry-and-cell-sorting-in-immunological-studies
#10
Andrea Cossarizza, Hyun-Dong Chang, Andreas Radbruch, Mübeccel Akdis, Immanuel Andrä, Francesco Annunziato, Petra Bacher, Vincenzo Barnaba, Luca Battistini, Wolfgang M Bauer, Sabine Baumgart, Burkhard Becher, Wolfgang Beisker, Claudia Berek, Alfonso Blanco, Giovanna Borsellino, Philip E Boulais, Ryan R Brinkman, Martin Büscher, Dirk H Busch, Timothy P Bushnell, Xuetao Cao, Andrea Cavani, Pratip K Chattopadhyay, Qingyu Cheng, Sue Chow, Mario Clerici, Anne Cooke, Antonio Cosma, Lorenzo Cosmi, Ana Cumano, Van Duc Dang, Derek Davies, Sara De Biasi, Genny Del Zotto, Silvia Della Bella, Paolo Dellabona, Günnur Deniz, Mark Dessing, Andreas Diefenbach, James Di Santo, Francesco Dieli, Andreas Dolf, Vera S Donnenberg, Thomas Dörner, Götz R A Ehrhardt, Elmar Endl, Pablo Engel, Britta Engelhardt, Charlotte Esser, Bart Everts, Anita Dreher, Christine S Falk, Todd A Fehniger, Andrew Filby, Simon Fillatreau, Marie Follo, Irmgard Förster, John Foster, Gemma A Foulds, Paul S Frenette, David Galbraith, Natalio Garbi, Maria Dolores García-Godoy, Jens Geginat, Kamran Ghoreschi, Lara Gibellini, Christoph Goettlinger, Carl S Goodyear, Andrea Gori, Jane Grogan, Mor Gross, Andreas Grützkau, Daryl Grummitt, Jonas Hahn, Quirin Hammer, Anja E Hauser, David L Haviland, David Hedley, Guadalupe Herrera, Martin Herrmann, Falk Hiepe, Tristan Holland, Pleun Hombrink, Jessica P Houston, Bimba F Hoyer, Bo Huang, Christopher A Hunter, Anna Iannone, Hans-Martin Jäck, Beatriz Jávega, Stipan Jonjic, Kerstin Juelke, Steffen Jung, Toralf Kaiser, Tomas Kalina, Baerbel Keller, Srijit Khan, Deborah Kienhöfer, Thomas Kroneis, Désirée Kunkel, Christian Kurts, Pia Kvistborg, Joanne Lannigan, Olivier Lantz, Anis Larbi, Salome LeibundGut-Landmann, Michael D Leipold, Megan K Levings, Virginia Litwin, Yanling Liu, Michael Lohoff, Giovanna Lombardi, Lilly Lopez, Amy Lovett-Racke, Erik Lubberts, Burkhard Ludewig, Enrico Lugli, Holden T Maecker, Glòria Martrus, Giuseppe Matarese, Christian Maueröder, Mairi McGrath, Iain McInnes, Henrik E Mei, Fritz Melchers, Susanne Melzer, Dirk Mielenz, Kingston Mills, David Mirrer, Jenny Mjösberg, Jonni Moore, Barry Moran, Alessandro Moretta, Lorenzo Moretta, Tim R Mosmann, Susann Müller, Werner Müller, Christian Münz, Gabriele Multhoff, Luis Enrique Munoz, Kenneth M Murphy, Toshinori Nakayama, Milena Nasi, Christine Neudörfl, John Nolan, Sussan Nourshargh, José-Enrique O'Connor, Wenjun Ouyang, Annette Oxenius, Raghav Palankar, Isabel Panse, Pärt Peterson, Christian Peth, Jordi Petriz, Daisy Philips, Winfried Pickl, Silvia Piconese, Marcello Pinti, A Graham Pockley, Malgorzata Justyna Podolska, Carlo Pucillo, Sally A Quataert, Timothy R D J Radstake, Bartek Rajwa, Jonathan A Rebhahn, Diether Recktenwald, Ester B M Remmerswaal, Katy Rezvani, Laura G Rico, J Paul Robinson, Chiara Romagnani, Anna Rubartelli, Beate Ruckert, Jürgen Ruland, Shimon Sakaguchi, Francisco Sala-de-Oyanguren, Yvonne Samstag, Sharon Sanderson, Birgit Sawitzki, Alexander Scheffold, Matthias Schiemann, Frank Schildberg, Esther Schimisky, Stephan A Schmid, Steffen Schmitt, Kilian Schober, Thomas Schüler, Axel Ronald Schulz, Ton Schumacher, Cristiano Scotta, T Vincent Shankey, Anat Shemer, Anna-Katharina Simon, Josef Spidlen, Alan M Stall, Regina Stark, Christina Stehle, Merle Stein, Tobit Steinmetz, Hannes Stockinger, Yousuke Takahama, Attila Tarnok, ZhiGang Tian, Gergely Toldi, Julia Tornack, Elisabetta Traggiai, Joe Trotter, Henning Ulrich, Marlous van der Braber, René A W van Lier, Marc Veldhoen, Salvador Vento-Asturias, Paulo Vieira, David Voehringer, Hans-Dieter Volk, Konrad von Volkmann, Ari Waisman, Rachael Walker, Michael D Ward, Klaus Warnatz, Sarah Warth, James V Watson, Carsten Watzl, Leonie Wegener, Annika Wiedemann, Jürgen Wienands, Gerald Willimsky, James Wing, Peter Wurst, Liping Yu, Alice Yue, Qianjun Zhang, Yi Zhao, Susanne Ziegler, Jakob Zimmermann
No abstract text is available yet for this article.
October 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/28974500/sex-differences-in-circulating-progenitor-cells
#11
Matthew L Topel, Salim S Hayek, Yi-An Ko, Pratik B Sandesara, Ayman Samman Tahhan, Iraj Hesaroieh, Ernestine Mahar, Greg S Martin, Edmund K Waller, Arshed A Quyyumi
BACKGROUND: Lower levels of circulating progenitor cells (PCs) reflect impaired endogenous regenerative capacity and are associated with aging, vascular disease, and poor outcomes. Whether biologic sex and sex hormones influence PC numbers remains a subject of controversy. We sought to determine sex differences in circulating PCs in both healthy persons and patients with coronary artery disease, and to determine their association with sex hormone levels. METHODS AND RESULTS: In 642 participants (mean age 48 years, 69% women, 23% black) free from cardiovascular disease, we measured circulating PC counts as CD45(med+) mononuclear cells coexpressing CD34 and its subsets expressing CD133, chemokine (C-X-C motif) receptor 4, and vascular endothelial growth factor receptor 2 epitopes using flow cytometry...
October 3, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28936778/preferential-reduction-of-circulating-innate-lymphoid-cells-type-2-in-patients-with-common-variable-immunodeficiency-with-secondary-complications-is-part-of-a-broader-immune-dysregulation
#12
David Friedmann, Baerbel Keller, Ina Harder, Jonas Schupp, Yakup Tanriver, Susanne Unger, Klaus Warnatz
PURPOSE: Over a third of patients with common variable immunodeficiency (CVID) suffer from secondary complications like inflammatory organ disease, autoimmune manifestations, or lymphoproliferation contributing to increased morbidity and mortality in affected patients. Innate lymphoid cells (ILCs) have emerging roles in setting the milieu for physiological, but also pathological, immune responses and inflammation. We therefore sought to correlate the recently identified disturbed homeostasis of ILCs with alterations of the adaptive immune system in complex CVID patients (CVIDc)...
November 2017: Journal of Clinical Immunology
https://www.readbyqxmd.com/read/28919582/rapamycin-inhibits-expansion-of-cord-blood-derived-nk-and-t-cell
#13
Monireh Zare, Behnaz Valipour, Seyede Momeneh Mohammadi, Mohammad Nouri, Aliakbar Movassaghpour, Hojjatollah Nozad Charoudeh
BACKGROUND: The mammalian target of rapamycin (mTOR) is important in hematopoiesis. Despite the central role of mTOR in regulating the differentiation of immune cells, the effect of mTOR function on cord blood mononuclear cells is yet to be defined. OBJECTIVES: To evaluate the effect of mTOR inhibition, using rapamycin on the proliferation and apoptosis of cord blood mononuclear cells, as well as on the B and T cell expansion. METHODS: Cord blood mononuclear cells were cultured in the presence of IL-2, IL-7 and IL-15 cytokines and inhibited by rapamycin for 14 days...
September 2017: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/28901513/antitumor-activity-of-2%C3%A2-2e-%C3%A2-3-7%C3%A2-dimethyl%C3%A2-2-6%C3%A2-octadienyl-%C3%A2-6%C3%A2-methyl%C3%A2-2-5%C3%A2-cyclohexadiene%C3%A2-1-4%C3%A2-dione-isolated-from-the-aerial-part-of-atractylodes%C3%A2-macrocephala-in-hepatocellular-carcinoma
#14
Lei Li, Rui Zhao, Ying Li, Wen-Hui Wang
2‑[(2E)‑3,7‑dimethyl‑2,6‑octadienyl]‑6‑methy l‑2,5‑cyclohexadiene‑1,4‑dione (DMD) is a compound isolated from Atractylodes macrocephala; however, its antitumor activity has not yet been investigated. Therefore, the present study aimed to investigate the antitumor activity of DMD in the H22 mouse hepatocellular carcinoma (HCC) cell line in vitro and in vivo. In the present study, the antiproliferative effects of DMD against H22 cells were evaluated using the MTT assay in vitro. Furthermore, xenograft nude mice were established to evaluate the antitumor effects of DMD on H22 cells in vivo...
November 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28899983/regulatory-nk-cells-mediated-between-immunosuppressive-monocytes-and-dysfunctional-t-cells-in-chronic-hbv-infection
#15
Haijun Li, Naicui Zhai, Zhongfeng Wang, Hongxiao Song, Yang Yang, An Cui, Tianyang Li, Guangyi Wang, Junqi Niu, Ian Nicholas Crispe, Lishan Su, Zhengkun Tu
BACKGROUND AND AIMS: HBV infection represents a major health problem worldwide, but the immunological mechanisms by which HBV causes chronic persistent infection remain only partly understood. Recently, cell subsets with suppressive features have been recognised among monocytes and natural killer (NK) cells. Here we examine the effects of HBV on monocytes and NK cells. METHODS: Monocytes and NK cells derived from chronic HBV-infected patients and healthy controls were purified and characterised for phenotype, gene expression and cytokines secretion by flow cytometry, quantitative real-time (qRT)-PCR, ELISA and western blotting...
September 12, 2017: Gut
https://www.readbyqxmd.com/read/28881492/a-novel-elane-mutation-associated-with-inflammatory-arthritis-defective-netosis-and-recurrent-parvovirus-infection
#16
Uma Thanarajasingam, Mark A Jensen, Jessica M Dorschner, Theresa Wampler Muskardin, Yogita Ghodke-Puranik, Monica Purmalek, Elias Eliopoulos, Maria I Zervou, George N Goulielmos, Matthew Howard, Mariana J Kaplan, Timothy B Niewold
OBJECTIVE: We describe a 38-year-old woman who presented with a history of inflammatory arthritis, rash, and daily fevers. She was noted to have chronic parvovirus infection with persistently detectable viral titers and a novel mutation in the ELANE gene. ELANE encodes neutrophil elastase, a neutrophil serine protease with important antimicrobial effects, and is found as part of neutrophil extracellular trap (NET) complexes. Pathogenic ELANE mutations have been identified in forms of hereditary neutropenia...
September 7, 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/28876451/selective-hdac6-inhibition-decreases-early-stage-of-lupus-nephritis-by-downregulating-both-innate-and-adaptive-immune-responses
#17
Jingjing Ren, Xiaofeng Liao, Miranda D Vieson, Miao Chen, Reilly Scott, Jillian Kazmierczak, Xin M Luo, Christopher M Reilly
We have previously demonstrated that HDAC6 expression is increased in animal models of systemic lupus erythematosus (SLE) and that inhibition of HDAC6 decreased disease. In our current studies, we tested if an orally active selective HDAC6 inhibitor would decrease disease pathogenesis in a lupus mouse model with established early disease. Additionally, we sought to delineate the cellular and molecular mechanism(s) of action of a selective HDAC6 inhibitor in SLE. We treated 20-week-old (early-disease) NZB/W F1 female mice with two different doses of the selective HDAC6 inhibitor (ACY-738) for five weeks...
September 6, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28843352/effects-of-rehabilitation-training-on-apoptosis-of-nerve-cells-and-the-recovery-of-neural-and-motor-functions-in-rats-with-ischemic-stroke-through-the-pi3k-akt-and-nrf2-are-signaling-pathways
#18
Xiao-Fei Jin, Shan Wang, Min Shen, Xin Wen, Xin-Rui Han, Jun-Chang Wu, Gao-Zhuo Tang, Dong-Mei Wu, Jun Lu, Yuan-Lin Zheng
This study was designed in order to investigate the effects between rehabilitation training on the apoptosis of nerve cells and the recovery of neural and motor functions of rats with ischemic stroke by way of the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) and nuclear factor E2-related factor 2/antioxidant responsive element (Nrf2/ARE) signaling pathways. In total, 110 healthy adult male Sprague-Dawley (SD) rats were selected in order to take part in this study. Ninety SD rats were used in order to establish the middle cerebral artery occlusion (MCAO), among which 80 rats were randomly assigned as part of the natural recovery, natural recovery+Rp-PI3K (the rats injected with PI3K/Akt inhibitor LY294002), rehabilitation training, and rehabilitation training+Rp-PI3K groups...
August 24, 2017: Brain Research Bulletin
https://www.readbyqxmd.com/read/28814673/human-lung-tumor-foxp3-tregs-upregulate-four-treg-locking-transcription-factors
#19
Tatiana Akimova, Tianyi Zhang, Dmitri Negorev, Sunil Singhal, Jason Stadanlick, Abhishek Rao, Michael Annunziata, Matthew H Levine, Ulf H Beier, Joshua M Diamond, Jason D Christie, Steven M Albelda, Evgeniy B Eruslanov, Wayne W Hancock
Experimental data indicate that FOXP3+ Tregs can markedly curtail host antitumor immune responses, but the properties of human intratumoral Tregs are still largely unknown, in part due to significant methodologic problems. We studied the phenotypic, functional, epigenetic, and transcriptional features of Tregs in 92 patients with non-small-cell lung cancer, comparing the features of Tregs within tumors versus corresponding blood, lung, and lymph node samples. Intratumoral Treg numbers and suppressive function were significantly increased compared with all other sites but did not display a distinctive phenotype by flow cytometry...
August 17, 2017: JCI Insight
https://www.readbyqxmd.com/read/28800964/the-effect-of-metal-ions-released-from-different-dental-implant-abutment-couples-on-osteoblast-function-and-secretion-of-bone-resorbing-mediators
#20
Ghada O Alrabeah, Peter Brett, Jonathan C Knowles, Haralampos Petridis
OBJECTIVES: The etiology of the reduced marginal bone loss observed around platform-switched implant-abutment connections is not clear but could be related to the release of variable amounts of corrosion products. The present study evaluated the effect of different concentrations of metal ions released from different implant abutment couples on osteoblastic cell viability, apoptosis and expression of genes related to bone resorption. METHODS: Osteoblastic cells were exposed to five conditions of culture media prepared containing metal ions (titanium, aluminum, vanadium, cobalt, chromium and molybdenum) in different concentrations representing the amounts released from platform-matched and platform-switched implant-abutment couples as a result of an earlier accelerated corrosion experiment...
November 2017: Journal of Dentistry
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