keyword
MENU ▼
Read by QxMD icon Read
search

cytometry part b

keyword
https://www.readbyqxmd.com/read/28668464/low-level-exposure-to-inorganic-mercury-interferes-with-b-cell-receptor-signaling-in-transitional-type-1-b-cells
#1
R Gill, M J McCabe, A J Rosenspire
Mercury (Hg) has been implicated as a factor contributing to autoimmune disease in animal models and humans. However the mechanism by which this occurs has remained elusive. Since the discovery of B cells it has been appreciated by immunologists that during the normal course of B cell development, some immature B cells must be generated that produce immunoglobulin reactive to self-antigens (auto-antibodies). However in the course of normal development, the vast majority of immature auto-reactive B cells are prevented from maturing by processes collectively known as tolerance...
June 28, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28666430/derivation-of-therapeutic-lung-spheroid-cells-from-minimally-invasive-transbronchial-pulmonary-biopsies
#2
Phuong-Uyen C Dinh, Jhon Cores, M Taylor Hensley, Adam C Vandergriff, Junnan Tang, Tyler A Allen, Thomas G Caranasos, Kenneth B Adler, Leonard J Lobo, Ke Cheng
BACKGROUND: Resident stem and progenitor cells have been identified in the lung over the last decade, but isolation and culture of these cells remains a challenge. Thus, although these lung stem and progenitor cells provide an ideal source for stem-cell based therapy, mesenchymal stem cells (MSCs) remain the most popular cell therapy product for the treatment of lung diseases. Surgical lung biopsies can be the tissue source but such procedures carry a high risk of mortality. METHODS: In this study we demonstrate that therapeutic lung cells, termed "lung spheroid cells" (LSCs) can be generated from minimally invasive transbronchial lung biopsies using a three-dimensional culture technique...
June 30, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28660746/pronase-treatment-improves-flow-cytometry-crossmatching-results
#3
M-J Apithy, J Desoutter, A Gicquel, E Guiheneuf, P-F Westeel, A Lesage, V Piot, G Choukroun, N Guillaume
Flow cytometry crossmatching (FC-XM) is the most sensitive cell-based method for detecting donor-specific antibodies in clinical organ transplantation. Unfortunately, background FC-XM reactivity is elevated in assays with B lymphocytes-partly because of nonspecific immunoglobulin binding by Fc receptors and B-cell surface immunoglobulins. To reduce the background reactivity in a B-cell FC-XM assay, we treated lymphocytes with pronase (1 mg/mL for 30 minutes). This treatment drastically reduced the presence of kappa light chains and Fc receptors (CD32b), while the concomitant decrease in CD19, CD20 and major histocompatibility complex (MHC) I and II expression on B-cells was acceptable...
June 28, 2017: HLA
https://www.readbyqxmd.com/read/28620656/oxidative-t-cell-modifications-in-lupus-and-sjogren-s-syndrome
#4
F M Strickland, T Mau, M O'Brien, A Ghosh, B C Richardson, R Yung
OBJECTIVES: Lupus flares are triggered by environmental agents that cause oxidative stress, but the mechanisms involved are unclear. The flares are characterized by oxidative modifications of proteins by 4-hydroxynonenals, malondialdehydes, carbonyls and nitration. These modifications have been proposed to induce and perpetuate lupus flares by "altered self" mechanisms. An epigenetically altered CD4+CD28+ T cell subset, caused at least in part by nitration of T cell signaling molecules, is found in patients with active lupus, and nitrated T cells are sufficient to cause lupus-like autoimmunity in animal models...
April 2017: Lupus (Los Angeles)
https://www.readbyqxmd.com/read/28583764/proteomic-composition-and-immunomodulatory-properties-of-urinary-bladder-matrix-scaffolds-in-homeostasis-and-injury
#5
REVIEW
Kaitlyn Sadtler, Sven D Sommerfeld, Matthew T Wolf, Xiaokun Wang, Shoumyo Majumdar, Liam Chung, Dhanashree S Kelkar, Akhilesh Pandey, Jennifer H Elisseeff
Urinary bladder matrix (UBM) is used clinically for management of wounds and reinforcement of surgical soft tissue repair, among other applications. UBM consists of the lamina propria and basal lamina of the porcine urinary bladder, and is decellularized as part of the process to manufacture the medical device. UBM is composed mainly of Collagen I, but also contains a wide variety of fibrillar and basement membrane collagens, glycoproteins, proteoglycans and ECM-associated factors. Upon application of the biomaterial in a traumatic or non-traumatic setting in a mouse model, there is a cascade of immune cells that respond to the damaged tissue and biomaterial...
June 2, 2017: Seminars in Immunology
https://www.readbyqxmd.com/read/28557280/cd8-expression-in-b-chronic-lymphocytic-leukemia-a-case-of-immuno-phenotypic-aberrancy
#6
Mili Jain, Rashmi Kushwaha, Shailendra Prasad Verma, Uma Shankar Singh, Anil Kumar Tripathi, Madhu Mati Goel, Ashutosh Kumar
Immuno-phenotyping forms an integral part of laboratory work up of Chronic lymphocytic leukemia (CLL). Aberrant antigen expressions are a known phenomenon in leukemic blasts, however cross lineage antigen expression is rarely seen in mature B cell leukemia, the reported percentages varying from 1% to 3% in Europe and North America. CASE DETAILS: We report a case of Rai stage 1 CLL showing aberrant expression of CD8 on flow cytometry. The patient had stable disease at a follow up of 9 months with no requirement of initiation of treatment...
May 30, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28542787/understanding-the-reconstitution-of-the-b-cell-compartment-in-bone-marrow-and-blood-after-treatment-for-b-cell-precursor-acute-lymphoblastic-leukaemia
#7
Prisca M J Theunissen, Anouk van den Branden, Alita Van Der Sluijs-Gelling, Valerie De Haas, Auke Beishuizen, Jacques J M van Dongen, Vincent H J Van Der Velden
A better understanding of the reconstitution of the B-cell compartment during and after treatment in B-cell precursor acute lymphoblastic leukaemia (BCP-ALL) will help to assess the immunological status and needs of post-treatment BCP-ALL patients. Using 8-colour flow cytometry and proliferation-assays, we studied the composition and proliferation of both the B-cell precursor (BCP) population in the bone marrow (BM) and mature B-cell population in peripheral blood (PB) during and after BCP-ALL therapy. We found a normal BCP differentiation pattern and a delayed formation of classical CD38(dim) -naive mature B-cells, natural effector B-cells and memory B-cells in patients after chemotherapy...
May 25, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28505514/tlr4-supports-the-expansion-of-fasl-cd5-cd1d-hi-regulatory-b-cells-which-decreases-in-contact-hypersensitivity
#8
Keng Wang, Lei Tao, Jianbing Su, Yueyang Zhang, Binhua Zou, Yiyuan Wang, Min Zou, Nana Chen, Linsheng Lei, Xiaojuan Li
Certain B cells termed as "regulatory B cells" (Bregs) can suppress the ongoing immune responses and a splenic CD5(+)CD1d(hi) Breg subset identified earlier was shown to exert its regulatory functions through secretion of IL-10. Though FasL expression is an alternative mechanism of immune suppression used by B cells, little is known about the FasL expressing CD5(+)CD1d(hi) Bregs. In this study, we isolated splenocytes or splenic CD19(+) B cells and compared the efficiency of toll-like receptor(TLR)4 ligand (lipopolysaccharide) with TLR9 ligand (CpG), anti-CD40 and TLR9 ligand (CpG) plus anti-CD40 on the FasL expression of splenic CD5(+)CD1d(hi) Bregs by flow cytometry...
May 12, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28488961/effect-of-storage-period-of-red-blood-cell-suspensions-on-helper-t-cell-subpopulations
#9
Salih H Bal, Yasemin Heper, Levent T Kumaş, Furkan Guvenc, Ferah Budak, Güher Göral, Haluk B Oral
BACKGROUND: The aim of this study was to investigate the immunological alterations that occur during the storage of erythrocyte suspensions which may lead to transfusion-related immunomodulation following allogeneic blood transfusion. MATERIALS AND METHODS: One part of the erythrocyte suspensions obtained from donors was leucoreduced while the other part was not. The leucoreduced (LR) and non-leucoreduced (NL) erythrocyte suspensions were then further divided into three equal amounts which were stored for 0, 21 or 42 days prior to measurements, by enzyme-linked immunosorbent assays, of cytokine levels in their supernatants...
March 15, 2017: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/28479724/cytotoxic-metabolites-from-callyspongia-siphonella-display-antiproliferative-activity-by-inducing-apoptosis-in-hct-116-cells
#10
Tariq R A Sobahi, Seif-Eldin N Ayyad, Ahmed Abdel-Lateff, Mardi M Algandaby, Hajer S Alorfi, Ashraf B Abdel-Naim
OBJECTIVES: To evaluate the antiproliferative effect of the isolated metabolites from Callyspongia siphonella. METHODS: Different chromatographic methods have been done on the organic extract of the marine sponge aiming at isolating the bioactive metabolites. The cytotoxicity of the isolated compounds has been evaluated against the human colorectal cancer cell line; HCT-116, employing SRB assay. The flow cytometry assay was applied to measure the cell cycle analysis...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28475275/a-qa-program-for-mrd-testing-demonstrates-that-systematic-education-can-reduce-discordance-among-experienced-interpreters
#11
Michael Keeney, Brent L Wood, Benjamin D Hedley, Joseph A DiGiuseppe, Maryalice Stetler-Stevenson, Elisabeth Paietta, Gerard Lozanski, Adam C Seegmiller, Bruce W Greig, Aaron C Shaver, Lata Mukundan, Howard R Higley, Caroline C Sigman, Gary Kelloff, J Milburn Jessup, Michael J Borowitz
BACKGROUND: Minimal residual disease (MRD) in B lymphoblastic leukemia (B-ALL) by flow cytometry is an established prognostic factor used to adjust treatment in most pediatric therapeutic protocols. MRD in B-ALL has been standardized by the Children's Oncology Group (COG) in North America, but not routine clinical labs. The Foundation for National Institutes of Health sought to harmonize MRD measurement among COG, oncology groups, academic, community and government, laboratories. METHODS: Listmode data from post-induction marrows were distributed from a reference lab to seven different clinical FCM labs with variable experience in B-ALL MRD...
May 5, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28416006/leishmania-major-large-rab-gtpase-is-highly-immunogenic-in-individuals-immune-to-cutaneous-and-visceral-leishmaniasis
#12
Rym Chamakh-Ayari, Mehdi Chenik, Ahmed Sahbi Chakroun, Narges Bahi-Jaber, Karim Aoun, Amel Meddeb-Garnaoui
BACKGROUND: We previously identified a Leishmania (L.) major large RAB GTPase (LmlRAB), a new atypical RAB GTPase protein. It is highly conserved in Leishmania species while displaying low level of homology with mammalian homologues. Leishmania small RAB GTPases proteins have been involved in regulation of exocytic and endocytic pathways whereas the role of large RAB GTPases proteins has not been characterized yet. We report here the immunogenicity of both recombinant rLmlRAB and rLmlRABC, in individuals with immunity against L...
April 17, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28406322/mass-spectrometry-based-proteomic-exploration-of-the-human-immune-system-focus-on-the-inflammasome-global-protein-secretion-and-t-cells
#13
Tuula A Nyman, Martina B Lorey, Wojciech Cypryk, Sampsa Matikainen
The immune system is our defense system against microbial infections and tissue injury, and understanding how it works in detail is essential for developing drugs for different diseases. Mass spectrometry-based proteomics can provide in-depth information on the molecular mechanisms involved in immune responses. Areas covered: Summarized are the key immunology findings obtained with MS-based proteomics in the past five years, with a focus on inflammasome activation, global protein secretion, mucosal immunology, immunopeptidome and T cells...
May 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/28400196/association-of-ribonuclease-t2-gene-polymorphisms-with-decreased-expression-and-clinical-characteristics-of-severity-in%C3%A2-crohn-s-disease
#14
Rivkah Gonsky, Phillip Fleshner, Richard L Deem, Eva Biener-Ramanujan, Dalin Li, Alka A Potdar, Janine Bilsborough, Shaohong Yang, Dermot P B McGovern, Stephan R Targan
BACKGROUND & AIMS: Variants in the tumor necrosis factor superfamily member 15 gene (TNFSF15, also called TL1A) have been associated with risk for inflammatory bowel disease (IBD). TL1A affects expression of multiple cytokines to promote mucosal inflammation. Little is known about the TL1A-response pathways that regulate cytokine expression. We investigated T-cell gene expression patterns to determine the mechanisms by which TL1A regulates cytokine production, and whether these associate with outcomes of patients with Crohn's disease (CD)...
July 2017: Gastroenterology
https://www.readbyqxmd.com/read/28360016/fc%C3%AE-riib-expression-on-b-cells-is-associated-with-treatment-efficacy-for-acute-rejection-after-kidney-transplantation
#15
Juan Jin, Jianguang Gong, Bo Lin, Yiwen Li, Qiang He
BACKGROUND: Fcγ receptors (FcγR) play a role in the acute rejection (AR) of organ transplants. FcγRIIB is an inhibitory FcγR expressed on B cells. Intravenous IgG (IVIG) and CD28 monoclonal antibody (mAb) have been shown to have immunomodulatory properties against AR. AIM: To examine the association between FcγRIIB expression on B cell subpopulations and AR treatment efficacy. METHODS: Male F344 rats were used as kidney donors and Lewis rats as recipients to establish models of renal transplantation...
May 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28326965/hematogones-detected-by-flow-cytometry-in-a-child-with-vitamin-b12-deficiency
#16
Lisa Sutton, Nkechi Mba
Vitamin B12 deficiency is a known cause of megaloblastic anemia and bone marrow failure. Bone marrow biopsies are not frequently performed as part of the diagnostic workup and can demonstrate morphologic features that overlap with myelodysplastic syndrome (MDS) and acute leukemia. We describe a case of a dysplastic bone marrow with increased bone marrow hematogones detected by flow cytometry in a child with vitamin B12 deficiency. Hematogones are normal B cell precursors, and hyperplasia has been described in a variety of often reactive conditions and also disease...
March 2017: Pediatric and Developmental Pathology
https://www.readbyqxmd.com/read/28298605/cxcr5-dependent-entry-of-cd8-t-cells-into-rhesus-macaque-b-cell-follicles-achieved-through-t-cell-engineering
#17
Victor I Ayala, Claire Deleage, Matthew T Trivett, Sumiti Jain, Lori V Coren, Matthew W Breed, Joshua A Kramer, James A Thomas, Jacob D Estes, Jeffrey D Lifson, David E Ott
Follicular helper CD4 T cells, TFH, residing in B-cell follicles within secondary lymphoid tissues, are readily infected by AIDS viruses and are a major source of persistent virus despite relative control of viral replication. This persistence is due at least in part to a relative exclusion of effective antiviral CD8 T cells from B-cell follicles. To determine whether CD8 T cells could be engineered to enter B-cell follicles, we genetically modified unselected CD8 T cells to express CXC chemokine receptor 5 (CXCR5), the chemokine receptor implicated in cellular entry into B-cell follicles...
June 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28284328/memory-b-cells-in-guillain-barr%C3%A3-syndrome
#18
Qian Wang, Chunye Xing, Yanlei Hao, Qiguang Shi, Ziyou Qi, Zhanyun Lv, Yan Song, Peng Xu, Xungang Feng, Lili Zhang, Yong Zhang, Yuzhong Wang, Nobuhiro Yuki
IgG autoantibodies against gangliosides show the highest titers at the disease onset of axonal Guillain-Barré syndrome (GBS), in which there are no IgM anti-ganglioside antibodies. We hypothesized that memory B cells take part in the development of producing IgG autoantibodies. In this study, we analyzed the memory B cells in patients with GBS using flow cytometry. There was significantly higher percentage of memory B cells in patients with GBS than the healthy controls. The Spearman correlation analysis demonstrated that increased percentage of memory B cells was positively correlated with the clinical severity of the patients with GBS...
April 15, 2017: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/28257055/a-novel-polysaccharide-conjugate-from-bullacta-exarata-induces-g1-phase-arrest-and-apoptosis-in-human-hepatocellular-carcinoma-hepg2-cells
#19
Ningbo Liao, Liang Sun, Jiang Chen, Jianjun Zhong, Yanjun Zhang, Ronghua Zhang
Bullacta exarata has been consumed in Asia, not only as a part of the normal diet, but also as a traditional Chinese medicine with liver- and kidney-benefitting functions. Several scientific investigations involving extraction of biomolecules from this mollusk and pharmacological studies on their biological activities have been carried out. However, little is known regarding the antitumor properties of polysaccharides from B. exarata, hence the polysaccharides from B. exarata have been investigated here. One polysaccharide conjugate BEPS-IA was isolated and purified from B...
March 1, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28249810/dynamic-changes-of-laboratory-parameters-and-peripheral-blood-lymphocyte-subsets-in-severe-fever-with-thrombocytopenia-syndrome-patients
#20
Jingwen Liu, Li Wang, Zhaolei Feng, Daying Geng, Ye Sun, Guangying Yuan
OBJECTIVES: The aim of this study was to dynamically investigate laboratory parameters and peripheral blood lymphocyte subsets in severe fever with thrombocytopenia syndrome (SFTS) patients at different stages, to evaluate the significance of these changes in the infection process and its influence on prognosis. METHODS: Case-control study was used in the research. Sixty-nine confirmed thrombocytopenia syndrome virus(SFTSV) infected patients were enrolled. They were divided into two groups, recovery group and poor prognosis group, according to the clinical prognosis of the diseases...
May 2017: International Journal of Infectious Diseases: IJID
keyword
keyword
114980
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"