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cytometry part b

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https://www.readbyqxmd.com/read/29771415/lncrna-snhg7-promotes-the-proliferation-of-esophageal-cancer-cells-and-inhibits-its-apoptosis
#1
L-J Xu, X-J Yu, B Wei, H-X Hui, Y Sun, J Dai, X-F Chen
OBJECTIVE: Our research studied the expression of long noncoding RNA (lncRNA) SNHG7 in esophageal cancer cells and tissues. The effect of lncRNA SNHG7 on proliferation and apoptosis of esophageal cancer cells has been discussed. PATIENTS AND METHODS: Si-SNHG7 was transfected into esophageal cancer cells, and qRT-PCR was performed to detect the expression of lncRNA SNHG7 in esophageal cancer cells and tissues. The effect of SNHG7 on the proliferation of esophageal cancer cells was measured by CCK8 assay and plate cloning assay, respectively...
May 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29754509/-new-and-traditional-directions-in-the-biology-and-management-of-childhood-acute-lymphoblastic-leukemia
#2
Bálint Egyed, Gábor Kovács, Nóra Kutszegi, Andrea Rzepiel, Judit Csányiné Sági, Dániel János Erdélyi, Judit Müller, Ágnes Félné Semsei
Owing to clinical trials and improvement over the past few decades, the majority of children with acute lymphoblastic leukemia (ALL) survive by first-line chemotherapy and combat with the problems of returning to community. However, many patients may have severe acute or late therapeutic side effects, and the survival rate in some groups (e.g., patients with MLL rearrangements, hypodiploidy, IKZF1 mutation or early precursor T cell phenotype) is far behind the average. Innovative strategies in medical attendance provide better clinical outcomes for them: complete gene diagnostics, molecularly targeted anticancer treatment, immuno-oncology and immune cell therapy...
May 2018: Orvosi Hetilap
https://www.readbyqxmd.com/read/29745271/fetal-hemoglobin-in-the-maternal-circulation-contribution-of-fetal-red-blood-cells
#3
Mutaz Dana, Eitan Fibach
The major hemoglobin (Hb) during fetal life is fetal Hb (Hb F). It is mostly replaced by adult Hbs before birth and during the first year of life. In adults, where Hb F comprises <2.0% of the total Hb, it is not homogenously distributed among the red blood cells (RBCs) but is concentrated in a few RBCs, termed F-cells. Interestingly, for reasons that are unclear, Hb F increases in the maternal circulation during pregnancy. This increased Hb F could have two potential origins that are not mutually exclusive: A) maternal origin, due to inducing environment of Hb F in the maternal erythroid precursors; B) fetal origin, due to fetal cells crossing the placenta and entering the maternal circulation...
May 10, 2018: Hemoglobin
https://www.readbyqxmd.com/read/29720833/evidence-for-the-involvement-of-cox-2-vegf-and-pten-pl3k-akt-pathway-the-mechanism-of-oroxin-b-treated-liver-cancer
#4
Nan-Nan Li, Xian-Sheng Meng, Yong-Rui Bao, Shuai Wang, Tian-Jiao Li
Background: Oroxin B (OB) is one of flavonoids isolated from traditional Chinese herbal medicine Oroxylum indicum (L.) Vent. Recent studies suggest that flavonoids have obvious anti-liver tumors effect, but the precise molecular mechanism is still unclear. Objective: The current study was performed to investigate the antitumor effects of OB on human hepatoma cell line SMMC-772 and explore the part of molecular mechanisms in this process. Materials and Methods: MTT method, terminal deoxynucleotidyl transferase dUTP nick end labeling assay and flow cytometry were utilized to detect the inhibition of proliferation and the apoptosis after treating OB in of SMMC-7721 cells...
April 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29593719/human-milk-oligosaccharide-2-fucosyllactose-improves-innate-and-adaptive-immunity-in-an-influenza-specific-murine-vaccination-model
#5
Ling Xiao, Thea Leusink-Muis, Nienke Kettelarij, Ingrid van Ark, Bernadet Blijenberg, Nienke A Hesen, Bernd Stahl, Saskia A Overbeek, Johan Garssen, Gert Folkerts, Belinda Van't Land
Background: Human milk is uniquely suited to provide optimal nutrition and immune protection to infants. Human milk oligosaccharides are structural complex and diverse consisting of short chain and long chain oligosaccharides typically present in a 9:1 ratio. 2'-Fucosyllactose (2'FL) is one of the most prominent short chain oligosaccharides and is associated with anti-infective capacity of human milk. Aim: To determine the effect of 2'FL on vaccination responsiveness (both innate and adaptive) in a murine influenza vaccination model and elucidate mechanisms involved...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29590650/mir-27a-promotes-hemin-induced-erythroid-differentiation-of-k562-cells-by-targeting-cdc25b
#6
Dongsheng Wang, Si Si, Qiang Wang, Guangcheng Luo, Qin Du, Qi Liang, Xiaolan Guo, Guoyuan Zhang, Jiafu Feng, Zhengwei Leng
BACKGROUND/AIMS: MicroRNAs (miRNAs) play a crucial role in erythropoiesis. MiR-23a∼27a∼24-2 clusters have been proven to take part in erythropoiesis via some proteins. CDC25B (cell division control Cdc2 phosphostase B) is also the target of mir-27a; whether it regulates erythropoiesis and its mechanism are unknown. METHODS: To evaluate the potential role of miR-27a during erythroid differentiation, we performed miR-27a gain- and loss-of-function experiments on hemin-induced K562 cells...
March 22, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29576743/bisdemethoxycurcumin-and-its-cyclized-pyrazole-analogue-differentially-disrupt-lipopolysaccharide-signalling-in-human-monocyte-derived-macrophages
#7
Serena Tedesco, Morena Zusso, Laura Facci, Annalisa Trenti, Carlotta Boscaro, Federica Belluti, Gian Paolo Fadini, Stephen D Skaper, Pietro Giusti, Chiara Bolego, Andrea Cignarella
Several studies suggest that curcumin and related compounds possess antioxidant and anti-inflammatory properties including modulation of lipopolysaccharide- (LPS-) mediated signalling in macrophage cell models. We here investigated the effects of curcumin and the two structurally unrelated analogues GG6 and GG9 in primary human blood-derived macrophages as well as the signalling pathways involved. Macrophages differentiated from peripheral blood monocytes for 7 days were activated with LPS or selective Toll-like receptor agonists for 24 h...
2018: Mediators of Inflammation
https://www.readbyqxmd.com/read/29545911/impact-on-early-outcomes-and-immune-reconstitution-of-high-dose-post-transplant-cyclophosphamide-vs-anti-thymocyte-globulin-after-reduced-intensity-conditioning-peripheral-blood-stem-cell-allogeneic-transplantation
#8
Christelle Retière, Catherine Willem, Thierry Guillaume, Henri Vié, Laetitia Gautreau-Rolland, Emmanuel Scotet, Xavier Saulquin, Katia Gagne, Marie C Béné, Berthe-Marie Imbert, Beatrice Clemenceau, Pierre Peterlin, Alice Garnier, Patrice Chevallier
We have compared prospectively the outcome and immune reconstitution of patients receiving either post-transplant cyclophosphamide (PTCY) ( n = 30) or anti-thymocyte globulin ATG ( n = 15) as Graft-versus-host disease (GVHD) prophylaxis after reduced-intensity conditioning (RIC) allogeneic peripheral blood stem cell (PBSC) transplantation (allo-SCT). The outcome and immune reconstitution of patients receiving either of these two regimens were compared prospectively. This study allowed also to investigate the impact of PTCY between haplo-identical vs matched donors and of clofarabine as part of the RIC regimen...
February 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/29545835/expression-and-phosphorylation-of-foxo1-influences-cell-proliferation-and-apoptosis-in-the-gastrointestinal-stromal-tumor-cell-line-gist-t1
#9
Tao Wang, Hui Zhao, Hua Gao, Changming Zhu, Yao Xu, Liping Bai, Junbo Liu, Feng Yan
The mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways are activated during pathogenesis of gastrointestinal stromal tumors (GISTs). Forkhead box protein O1 (FOXO1) is a transcription factor regulated by the MAPK and PI3K pathways and is associated with multiple metabolic reactions. The present study aims to investigate the association of FOXO1 with cell proliferation and apoptosis in the cell line, GIST-T1. Cell counting kit-8 assay revealed that cell growth was inhibited by the PI3K inhibitor, LY294002, and/or MAPK inhibitor, UO126...
April 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29535184/the-invasin-d-protein-from-yersinia-pseudotuberculosis-selectively-binds-the-fab-region-of-host-antibodies-and-affects-colonization-of-the-intestine
#10
Pooja Sadana, Rebecca Geyer, Joern Pezoldt, Saskia Helmsing, Jochen Huehn, Michael Hust, Petra Dersch, Andrea Scrima
Yersinia pseudotuberculosis is a Gram-negative bacterium and zoonotic pathogen responsible for a wide range of diseases, ranging from mild diarrhea, enterocolitis, lymphatic adenitis to persistent local inflammation. The Y. pseudotuberculosis invasin D (InvD) molecule belongs to the invasin (InvA)-type autotransporter proteins, but its structure and function remain unknown. In this study, we present the first crystal structure of InvD, analyzed its expression and function in a murine infection model, and identified its target molecule in the host...
March 13, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29521959/forward-modeling-of-inherent-optical-properties-from-flow-cytometry-estimates-of-particle-size-and-refractive-index
#11
Jacopo Agagliate, Ina Lefering, David McKee
A Mie-based forward modeling procedure was developed to reconstruct bulk inherent optical properties (IOPs) from particle size distributions (PSDs) and real refractive index distributions (PRIDs) obtained using a previously developed flow cytometric (FC) method [Appl. Opt.57, 1705 (2018)APOPAI0003-693510.1364/AO.57.001705]. Given the available PSDs, extrapolations for the particle fraction outside the detection limits of the method and a complex refractive index input (with real part nr directly estimated and imaginary part ni adapted from the literature separately for organic and inorganic components), the model produces volume scattering functions that are integrated to produce scattering and backscattering coefficients, and absorption efficiencies that are used to calculate absorption coefficients...
March 10, 2018: Applied Optics
https://www.readbyqxmd.com/read/29474463/the-development-and-validation-of-different-decision-making-tools-to-predict-urine-culture-growth-out-of-urine-flow-cytometry-parameter
#12
Martin Müller, Ruth Seidenberg, Sabine K Schuh, Aristomenis K Exadaktylos, Clyde B Schechter, Alexander B Leichtle, Wolf E Hautz
OBJECTIVE: Patients presenting with suspected urinary tract infection are common in every day emergency practice. Urine flow cytometry has replaced microscopic urine evaluation in many emergency departments, but interpretation of the results remains challenging. The aim of this study was to develop and validate tools that predict urine culture growth out of urine flow cytometry parameter. METHODS: This retrospective study included all adult patients that presented in a large emergency department between January and July 2017 with a suspected urinary tract infection and had a urine flow cytometry as well as a urine culture obtained...
2018: PloS One
https://www.readbyqxmd.com/read/29474450/treatment-with-native-heterodimeric-il-15-increases-cytotoxic-lymphocytes-and-reduces-shiv-rna-in-lymph-nodes
#13
Dionysios C Watson, Eirini Moysi, Antonio Valentin, Cristina Bergamaschi, Santhi Devasundaram, Sotirios P Fortis, Jenifer Bear, Elena Chertova, Julian Bess, Ray Sowder, David J Venzon, Claire Deleage, Jacob D Estes, Jeffrey D Lifson, Constantinos Petrovas, Barbara K Felber, George N Pavlakis
B cell follicles in secondary lymphoid tissues represent an immune privileged sanctuary for AIDS viruses, in part because cytotoxic CD8+ T cells are mostly excluded from entering the follicles that harbor infected T follicular helper (TFH) cells. We studied the effects of native heterodimeric IL-15 (hetIL-15) treatment on uninfected rhesus macaques and on macaques that had spontaneously controlled SHIV infection to low levels of chronic viremia. hetIL-15 increased effector CD8+ T lymphocytes with high granzyme B content in blood, mucosal sites and lymph nodes, including virus-specific MHC-peptide tetramer+ CD8+ cells in LN...
February 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29464038/the-influence-of-chemotherapy-on-adenosine-producing-b-cells-in-patients-with-head-and-neck-squamous-cell-carcinoma
#14
Andreas Ziebart, Ulrich Huber, Sandra Jeske, Simon Laban, Johannes Doescher, Thomas K Hoffmann, Cornelia Brunner, Edwin K Jackson, Patrick J Schuler
Introduction: Head and neck squamous cell carcinoma (HNSCC) strongly suppresses the immune system, resulting in increased metastasis and recurrent disease. Chemotherapy is part of the multimodal treatment but may further immunosuppression. Recently, we demonstrated that regulatory B cells (Breg), defined as CD19+ CD39+ CD73+ B cells, play a significant role in the production of immunosuppressive, extracellular adenosine (ADO). Here, we tested the influence of chemotherapy on Breg function...
January 19, 2018: Oncotarget
https://www.readbyqxmd.com/read/29459702/qfmatch-multidimensional-flow-and-mass-cytometry-samples-alignment
#15
Darya Y Orlova, Stephen Meehan, David Parks, Wayne A Moore, Connor Meehan, Qian Zhao, Eliver E B Ghosn, Leonore A Herzenberg, Guenther Walther
Part of the flow/mass cytometry data analysis process is aligning (matching) cell subsets between relevant samples. Current methods address this cluster-matching problem in ways that are either computationally expensive, affected by the curse of dimensionality, or fail when population patterns significantly vary between samples. Here, we introduce a quadratic form (QF)-based cluster matching algorithm (QFMatch) that is computationally efficient and accommodates cases where population locations differ significantly (or even disappear or appear) from sample to sample...
February 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29438268/endocytosis-of-red-blood-cell-microparticles-by-pulmonary-endothelial-cells-is-mediated-by-rab5
#16
Young Kim, William A Abplanalp, Andrew D Jung, Rebecca M Schuster, Alex B Lentsch, Erich Gulbins, Charles C Caldwell, Timothy A Pritts
Microparticles are submicron vesicles shed from aging erythrocytes as a characteristic feature of the red blood cell (RBC) storage lesion. Exposure of pulmonary endothelial cells to RBC-derived microparticles promotes an inflammatory response, but the mechanisms underlying microparticle-induced endothelial cell activation are poorly understood. In the present study, cultured murine lung endothelial cells (MLECs) were treated with microparticles isolated from aged murine packed RBCs or vehicle. Microparticle-treated cells demonstrated increased expression of the adhesion molecules ICAM and E-selectin, as well as the cytokine, IL-6...
March 2018: Shock
https://www.readbyqxmd.com/read/29436594/uvb-induces-apoptosis-via-downregulation-of-calml3-dependent-jnk1-2-and-erk1-2-pathways-in-cataract
#17
Yin Jia, Qin Qin, Chao-Ping Fang, Wei Shen, Ting-Ting Sun, Yuan-Lan Huang, Wen-Jie Li, An-Mei Deng
The aim of the current study was to understand the mechanisms of apoptosis occurring in cultured human lens epithelial cells (HLECs) following ultraviolet B (UVB) irradiation. The investigations intended to confirm the presence of apoptosis and to reveal the roles of oxidative stress, calcium (Ca2+), c‑Jun NH2‑terminal kinase (JNK)1/2, and extracellular signal‑regulated kinase (ERK)1/2 signaling pathway in these progresses. Cell apoptosis, ROS generation and intracellular Ca2+ concentration was measured by flow cytometry...
May 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29422973/chloroquine-affects-autophagy-to-achieve-an-anticancer-effect-in-ec109-esophageal-carcinoma-cells-in-vitro
#18
Yan Cai, Jiajing Cai, Qiang Ma, Yuan Xu, Jiang Zou, Lei Xu, Dongsheng Wang, Xiaolan Guo
Esophageal carcinoma is a malignancy that severely threatens human health, with a high incidence rate and a low 5-year survival rate. Resistance to chemotherapy frequently emerges during its treatment, partly due to the induction of autophagy. Therefore, targeting autophagy may be a promising therapeutic approach for the treatment of esophageal carcinoma. In the present study, it was investigated how chloroquine (CQ) can influence the growth ability and biological behaviors of EC109 esophageal squamous carcinoma cells in vitro , as well as the potential molecular mechanisms behind its activity...
January 2018: Oncology Letters
https://www.readbyqxmd.com/read/29382365/increase-of-mzb1-in-b-cells-in-systemic-lupus-erythematosus-proteomic-analysis-of-biopsied-lymph-nodes
#19
Aya Miyagawa-Hayashino, Hajime Yoshifuji, Koji Kitagori, Shinji Ito, Takuma Oku, Yoshitaka Hirayama, Adeeb Salah, Toshiki Nakajima, Kaori Kiso, Norishige Yamada, Hironori Haga, Tatsuaki Tsuruyama
BACKGROUND: Systemic lupus erythematosus (SLE) is a prototypical autoimmune disease in which dysregulation of B cells has been recognized. Here, we searched for potential biomarkers of SLE using liquid chromatography-tandem mass spectrometry (LC-MS). METHODS: Lymph nodes from SLE patients and controls were analyzed by LC-MS. To validate the identified molecules, immunoblotting and immunohistochemistry were performed and B cells from SLE patients were analyzed by quantitative RT-PCR...
January 30, 2018: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/29375053/expression-of-purinergic-p2x-7-receptors-in-subpopulations-of-peripheral-blood-mononuclear-cells-in-early-stage-of-chronic-kidney-disease
#20
I Lajdova, A Oksa, M Horvathova, V Spustova
Chronic kidney disease (CKD) is known as a state of chronic low-grade inflammation, enhancing cardiovascular risk and immunodeficiency. Purinergic signaling has been accepted as a crucial component in the pathogenesis of various diseases, mediating a vast array of biological processes. The P2X7 receptor is one of the important cell surface regulators of several key inflammatory molecules. The aim of the study was to examine the expression of surface P2X7 receptors in subpopulations of peripheral blood mononuclear cells (PBMCs), and to evaluate the promising prognostic markers of inflammation (neutrophil/lymphocyte, Ne/Ly ratio) and cardiovascular risk (monocyte/high density lipoprotein cholesterol, Mo/HDL ratio) in early-stage CKD...
October 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
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