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cytometry part b

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https://www.readbyqxmd.com/read/27913304/effects-of-nongenetic-factors-on-immune-cell-dynamics-in-early-childhood-the-generation-r-study
#1
Diana van den Heuvel, Michelle A E Jansen, Kazem Nasserinejad, Willem A Dik, Ellen G van Lochem, Liesbeth E Bakker-Jonges, Halima Bouallouch-Charif, Vincent W V Jaddoe, Herbert Hooijkaas, Jacques J M van Dongen, Henriëtte A Moll, Menno C van Zelm
BACKGROUND: . Numbers of blood leukocyte subsets are highly dynamic in childhood and differ greatly between individuals. Inter-individual variation is only partly accounted for by genetic factors. OBJECTIVE: Determine which nongenetic factors affect the dynamics of innate leukocytes, and naive and memory lymphocyte subsets. METHODS: . We performed six-color flow cytometry and linear mixed effect modeling to define the dynamics of 62 leukocyte subsets from birth to 6 years of age in 1,182 children with one to five measurements per individual...
November 29, 2016: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27865301/resveratrol-inhibited-non-small-cell-lung-cancer-through-inhibiting-stat-3-signaling
#2
Xin Li, Dan Wang, Qing Chun Zhao, Tao Shi, Jun Chen
BACKGROUND: Resveratrol has demonstrated many beneficial effects against cancers; however, the mechanism remains unclear. Non-small cell lung cancer accounts for 80% of lung cancers. The present study was designed to observe the effects and related mechanisms of resveratrol on non-small cell lung cancer in in vitro A549 cells. MATERIALS AND METHODS: The anticancer effects of resveratrol were analyzed on cell viability, migration and invasion, proliferation and apoptosis...
November 2016: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/27863141/uvb-irradiation-causes-sting-dependent-production-of-protective-type-1-interferon-in-the-skin-by-recruited-inflammatory-monocytes
#3
Clayton Sontheimer, Denny Liggitt, Keith B Elkon
Objective Photosensitivity is common in patients with systemic lupus, though the mechanisms linking ultraviolet light to flares is not well understood. Here, we asked whether repetitive UVB exposure could induce Type I interferon (IFN-I) in normal mouse skin, and investigated the roles of inflammatory monocytes and plasmacytoid dendritic cells (PDCs) in IFN-I production and development of UVB-induced inflammation. Methods Mice were irradiated with UVB at 100 mJ/cm(2) for five days and cutaneous manifestations examined by mRNA expression of inflammatory and IFN-I response genes, histology, and flow cytometry...
November 11, 2016: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/27857021/effects-and-mechanisms-of-metformin-on-the-proliferation-of-esophageal-cancer-cells-in-vitro-and-in-vivo
#4
Jian-Cai Tang, Rui An, Yi-Qing Jiang, Jian Yang
Purpose: To observe the effects of metformin on human esophageal cancer cell, and to investigate its possible mechanisms. Materials and Methods: Cell viability was detected by using a cell counting kit-8(CCK-8), while cell cycle and apoptosis were assessed by flow cytometry and western blot was used to measure the expression of the related proteins. RNAi was used to knockout pyruvate kinase muscle isozyme 2 (PKM2). An Eca109 tumor model was established to evaluate the antitumor effect in vivo...
November 11, 2016: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/27852523/anemia-offers-stronger-protection-than-sickle-cell-trait-against-the-erythrocytic-stage-of-falciparum-malaria-and-this-protection-is-reversed-by-iron-supplementation
#5
M M Goheen, R Wegmüller, A Bah, B Darboe, E Danso, M Affara, D Gardner, J C Patel, A M Prentice, C Cerami
BACKGROUND: Iron deficiency causes long-term adverse consequences for children and is the most common nutritional deficiency worldwide. Observational studies suggest that iron deficiency anemia protects against Plasmodiumfalciparum malaria and several intervention trials have indicated that iron supplementation increases malaria risk through unknown mechanism(s). This poses a major challenge for health policy. We investigated how anemia inhibits blood stage malaria infection and how iron supplementation abrogates this protection...
November 9, 2016: EBioMedicine
https://www.readbyqxmd.com/read/27831501/characterizing-cd137-upregulation-on-nk-cells-in-patients-receiving-monoclonal-antibody-therapy
#6
A Makkouk, V Sundaram, C Chester, S Chang, A D Colevas, J B Sunwoo, H Maecker, M Desai, H E Kohrt
BACKGROUND: In the era of personalized cancer medicine, identifying techniques for effectively matching patients to efficacious treatments is a critical step in the treatment process. The advent of anti-cancer immunotherapies necessitates novel approaches to biomarker identification beyond traditional genomic profiling. One promising approach is incorporation of nomograms into treatment decisions. Nomograms are prediction tools, based on statistical modeling, designed to predict treatment outcomes...
November 9, 2016: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/27823624/antitumor-lignanamides-from-the-aerial-parts-of-corydalis-saxicola
#7
Bin Zhang, Rizhen Huang, Jing Hua, Hong Liang, Yingming Pan, Lumei Dai, Dong Liang, Hengshan Wang
BACKGROUND: Cancer is one of the leading cause of unnatural death globally. There is still a great need for effective anticancer agents from plant sources. Corydalis saxicola Bunting is a medicinal plant that is traditionally used to treat various diseases in southwest China. Previous phytochemical investigations of C. saxicola have focused on isoquinoline alkaloids that have been isolated, which have activity against anti-hepatitis B virus and inhibit DNA topoisomerase I. However, the exploration of other classes of constituents and their bioactivities needs further study...
December 1, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27777974/lymphocyte-activation-gene-3-and-coronary-artery-disease
#8
Diana Golden, Antonina Kolmakova, Sunitha Sura, Anthony T Vella, Ani Manichaikul, Xin-Qun Wang, Suzette J Bielinski, Kent D Taylor, Yii-Der Ida Chen, Stephen S Rich, Annabelle Rodriguez
BACKGROUND: The lipoprotein scavenger receptor BI (SCARB1) rs10846744 noncoding variant is significantly associated with atherosclerotic disease independently of traditional cardiovascular risk factors. We identified a potentially novel connection between rs10846744, the immune checkpoint inhibitor lymphocyte activation gene 3 (LAG3), and atherosclerosis. METHODS: In vitro approaches included flow cytometry, lipid raft isolation, phosphosignaling, cytokine measurements, and overexpressing and silencing LAG3 protein...
October 20, 2016: JCI Insight
https://www.readbyqxmd.com/read/27754580/comparison-and-optimization-of-machine-learning-methods-for-automated-classification-of-circulating-tumor-cells
#9
Timothy B Lannin, Fredrik I Thege, Brian J Kirby
Advances in rare cell capture technology have made possible the interrogation of circulating tumor cells (CTCs) captured from whole patient blood. However, locating captured cells in the device by manual counting bottlenecks data processing by being tedious (hours per sample) and compromises the results by being inconsistent and prone to user bias. Some recent work has been done to automate the cell location and classification process to address these problems, employing image processing and machine learning (ML) algorithms to locate and classify cells in fluorescent microscope images...
October 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27731950/elimination-of-erroneous-results-in-flow-cytometry-caused-by-antibody-binding-to-fc-receptors-on-human-monocytes-and-macrophages
#10
Morten N Andersen, Sinan N H Al-Karradi, Tue W Kragstrup, Marianne Hokland
Nonspecific binding of monoclonal antibodies (mAbs) to Fc-receptors on leukocytes is an important cause of background fluorescence in flow cytometry, and failing to block such nonspecific binding can lead to erroneous results. A major part of previous studies on blocking reagents for flow cytometry have been done in mice, and published results are not completely in agreement. In humans, Fc-receptors are found on most leukocytes, with highest abundance on monocytes/macrophages. Therefore, in the present study our aim was to thoroughly investigate the efficiency of different commonly used blocking reagents regarding inhibition of nonspecific binding of mouse mAbs to human peripheral blood mononuclear cells (MNCs) and monocyte-derived macrophages (MDMs)...
November 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27723268/polymorphisms-in-the-f-pocket-of-hla-b27-subtypes-strongly-impact-on-assembly-chaperone-interactions-and-heavy-chain-misfolding
#11
David B Guiliano, Helen North, Eleni Panayoitou, Elaine C Campbell, Kirsty McHugh, Fiona G M Cooke, Marine Silvestre, Paul Bowness, Simon J Powis, Antony N Antoniou
Objective - HLA-B27 is associated with the inflammatory spondyloarthropathies (SpAs). Of significance, subtypes HLA-B*27:06 and HLA-B*27:09 are not associated with the SpAs. These subtypes primarily differ from the HLA-B*27:05 disease associated allele at residues 114 and 116 of the heavy chain, part of the F pocket of the antigen-binding groove. Dimerisation of HLA-B27 during assembly has been implicated in disease onset. This study investigated the factors influencing differences in dimerisation between disease associated and non-associated HLA-B27 alleles...
October 9, 2016: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/27720959/tgf-%C3%AE-1-and-contact-mediated-suppression-by-cd4-cd25-cd127-t-regulatory-cells-of-patients-with-self-limiting-hepatitis-e
#12
Sanjay B Rathod, Anuradha S Tripathy
BACKGROUND AND AIM: Literature on the role of Regulatory T cells (Tregs) in acute viral infections is limited. Having established that the Tregs in self-limiting hepatitis E infection are elevated and functional, this study has focused on characterizing the specificity, phenotypes and identifying the molecules or factors responsible for enhancement of Treg cells and abrogation of Treg-mediated suppression in hepatitis E. METHODS: HEV rORF2p specific (a) Treg frequency, subset analysis and expression of surface and intracellular markers on Tregs and CFSE based functional analysis by flow cytometry (b) key cytokines quantification by multiplex (c) suppressive functional assay in the presence of anti-TGF-β1 or anti-IL-10 or both antibodies or Transwell insert or in combination were performed on samples from 58 acute patients (AVH-E), 45 recovered individuals from hepatitis E and 55 controls...
October 6, 2016: Human Immunology
https://www.readbyqxmd.com/read/27716906/decreased-level-of-il-10-producing-b-cells-in-patients-with-pemphigus-but-not-with-pemphigoid
#13
M Kabuto, N Fujimoto, T Takahashi, T Tanaka
BACKGROUND: hile the frequency of IL-10-producing B cells (B10 cells) is reported to have an inverse correlation with disease activity in some human autoimmune diseases, the association between B10 cells and autoimmune blistering diseases (AIBD) has not been well-evaluated. Although several phenotypes of human regulatory B cells have been proposed, the most appropriate one was not established. OBJECTIVE: This study aimed to evaluate B10 cells in AIBD including their phenotypes...
October 7, 2016: British Journal of Dermatology
https://www.readbyqxmd.com/read/27706765/role-of-il-10-and-tnf-%C3%AE-during-mycobacterium-tuberculosis-infection-in-murine-alveolar-macrophages
#14
J Shi, B H Sun, L R Zhou, X S Wang
Mycobacterium tuberculosis (Mtb) is known to be responsible for tuberculosis (TB), but the pathogenesis of this disease and the host defense mechanisms involved are, for the most part, poorly understood. In this study, we divided 30 male C57BL/6 mice into control and infection groups, and following injection with physiological saline or Mtb, respectively, euthanized five mice from each group on days 1, 3, and 7. TNF-α and IL-10 levels were measured by enzyme-linked immunosorbent assay and flow cytometry, with the latter also being performed to assess apoptosis rates...
September 23, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27591738/il28b-genetic-variation-and-cytomegalovirus-specific-t-cell-immunity-in-allogeneic-stem-cell-transplant-recipients
#15
Isabel Corrales, Carlos Solano, Paula Amat, Estela Giménez, Rafael de la Cámara, José Nieto, Javier López, Ana García-Noblejas, José Luis Piñana, David Navarro
A single nucleotide polymorphism (SNP), 3 kbp upstream of the IL28B gene (rs12979860; C/T), has been shown to influence the dynamics of cytomegalovirus (CMV) replication in allogeneic stem cell transplant recipients (Allo-SCT). We investigated whether this SNP had any effect on the dynamics of CMV-specific T-cell immunity in these patients. CMV pp65/IE-1 IFN-γ CD8(+) and CD4(+) T cells were enumerated by flow cytometry in 85 patients with no prior CMV DNAemia (group A) and in 57 after the onset of CMV DNAemia (group B)...
September 3, 2016: Journal of Medical Virology
https://www.readbyqxmd.com/read/27560943/cd52-negative-nk-cells-are-abundant-in-the-liver-and-less-susceptible-to-alemtuzumab-treatment
#16
Ryuichi Hotta, Masahiro Ohira, Toshiharu Matsuura, Izumi Muraoka, Panagiotis Tryphonopoulos, Ji Fan, Akin Tekin, Gennaro Selvaggi, David Levi, Phillip Ruiz, Camillo Ricordi, Rodrigo Vianna, Hideki Ohdan, Herman Waldmann, Andreas G Tzakis, Seigo Nishida
BACKGROUND: T-cell depleting strategies have become an integral part of immunosuppressive regimens in organ transplantation. Alemtuzumab is a humanized monoclonal antibody against CD52, a cell-surface antigen on several immune cells. It has been suggested that lymphocyte depletion increases the risk of serious infections. However, this has not been observed with short-term alemtuzumab treatment in an organ transplant setting. For induction therapy using alemtuzumab following liver transplantation, we found that T- and B-cell numbers declined rapidly after alemtuzumab therapy; however, the natural killer (NK) cell number was sustained...
2016: PloS One
https://www.readbyqxmd.com/read/27543462/signaling-effects-of-sodium-hydrosulfide-in-healthy-donor-peripheral-blood-mononuclear-cells
#17
André Sulen, Stein-Erik Gullaksen, Lucius Bader, David W McClymont, Jørn Skavland, Sonia Gavasso, Bjørn T Gjertsen
Hydrogen sulfide (H2S) is an endogenous gasotransmitter in human physiology and inflammatory disease, however, with limited knowledge of how signal transduction pathways are involved in immune cells. To examine the effects of sulfide on relevant intracellular signaling in human peripheral blood mononuclear cells (PBMCs), we stimulated healthy donor PBMCs with sodium hydrosulfide (NaHS, 1-1000μM) to mimic H2S stimulation, and analyzed phosphorylation of p38 mitogen activated protein kinase (MAPK) (pT180/pY182), NF-κB p65 (pS529), Akt (pS473) and CREB/ATF1 (pS133/pS63) with flow and mass cytometry...
August 16, 2016: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/27496496/banking-of-hematopoietic-stem-cells-influence-of-storage-time-on-their-quality-parameters
#18
A Kubiak, P Matuszak, E Bembnista, M Kozlowska-Skrzypczak
BACKGROUND: Banking of hematopoietic stem cells (HSCs) is a rapidly growing part of the transplant field. The essence of the banking process is to maintain the optimal quality parameters throughout the storage period, allowing successful transplantation. METHODS: Our laboratory research was carried out on 126 HSC samples that were collected by means of leukapheresis from patients with lymphoproliferative diseases. The samples were frozen in a controlled rate and stored up to 76 months in containers in vapor phase of liquid nitrogen...
June 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27467960/lenalidomide-enhances-myeloma-specific-t-cell-responses-in-vivo-and-in-vitro
#19
Isabelle Krämer, Melanie Engelhardt, Sabrina Fichtner, Brigitte Neuber, Sergej Medenhoff, Uta Bertsch, Jens Hillengass, Marc-Steffen Raab, Dirk Hose, Anthony D Ho, Hartmut Goldschmidt, Michael Hundemer
Immunomodulation is an important part of lenalidomide's mode of action. We analyzed the impact of lenalidomide on T cells from patients with multiple myeloma during lenalidomide therapy in vivo and in patients with lenalidomide-refractory disease in vitro Patients enrolled in the German Speaking Myeloma Multicenter Group (GMMG) MM5 trial received a consolidation therapy with two cycles of lenalidomide after autologous stem cell transplantation (ASCT). Half of the study population continued treatment with lenalidomide maintenance therapy for 2 y, while the other patients received lenalidomide maintenance therapy until complete remission...
May 2016: Oncoimmunology
https://www.readbyqxmd.com/read/27441238/inhibitory-effect-of-isoamericanol-a-from-jatropha-curcas-seeds-on-the-growth-of-mcf-7-human-breast-cancer-cell-line-by-g2-m-cell-cycle-arrest
#20
Ayako Katagi, Li Sui, Kazuyo Kamitori, Toshisada Suzuki, Takeshi Katayama, Akram Hossain, Chisato Noguchi, Youyi Dong, Fuminori Yamaguchi, Masaaki Tokuda
Although various parts of J. curcas (Jatropha curcas L., Euphorbiaceae) have long been used as traditional folk medicines for their antiviral, analgesic, and/or antidotal efficacies, we are the first to investigate the role of anti-carcinogenicity of isoamericanol A (IAA) from the seed extract. Our results showed that IAA is capable of inhibiting cell proliferation in a dose-dependent manner on the human cancer cell lines of MCF-7, MDA-MB231, HuH-7, and HeLa. Flow cytometry analysis showed IAA significantly induces cell cycle arrest at G2/M on MCF-7 cells...
January 2016: Heliyon
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