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biofilm and burkholderia

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https://www.readbyqxmd.com/read/28733286/the-regulator-ldhr-and-the-d-lactate-dehydrogenase-ldha-of-burkholderia-multivorans-play-a-role-in-carbon-overflow-and-in-planktonic-cellular-aggregates-formation
#1
Inês N Silva, Marcelo J Ramires, Lisa A Azevedo, Ana R Guerreiro, Andreia C Tavares, Jörg D Becker, Leonilde M Moreira
LysR-type transcriptional regulators (LTTR) are the most commonly found regulators in Burkholderia cepacia complex, comprising opportunistic pathogens causing chronic respiratory infections in cystic fibrosis (CF) patients. Despite LTTRs being global regulators of pathogenicity in several bacteria, few have been characterized in Burkholderia Here, we showed that gene ldhR of B. multivorans encoding a LTTR is co-transcribed with ldhA encoding a d-lactate dehydrogenase, and evaluate their implication in virulence traits like exopolysaccharide (EPS) synthesis and biofilm formation...
July 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28658258/genome-scale-analysis-of-the-genes-that-contribute-to-burkholderia-pseudomallei-biofilm-formation-identifies-a-crucial-exopolysaccharide-biosynthesis-gene-cluster
#2
Grace I Borlee, Brooke A Plumley, Kevin H Martin, Nawarat Somprasong, Mihnea R Mangalea, M Nurul Islam, Mary N Burtnick, Paul J Brett, Ivo Steinmetz, David P AuCoin, John T Belisle, Dean C Crick, Herbert P Schweizer, Bradley R Borlee
Burkholderia pseudomallei, the causative agent of melioidosis, is an important public health threat due to limited therapeutic options for treatment. Efforts to improve therapeutics for B. pseudomallei infections are dependent on the need to understand the role of B. pseudomallei biofilm formation and its contribution to antibiotic tolerance and persistence as these are bacterial traits that prevent effective therapy. In order to reveal the genes that regulate and/or contribute to B. pseudomallei 1026b biofilm formation, we screened a sequence defined two-allele transposon library and identified 118 transposon insertion mutants that were deficient in biofilm formation...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28651010/macrophages-but-not-neutrophils-are-critical-for-proliferation-of-burkholderia-cenocepacia-and-ensuing-host-damaging-inflammation
#3
Jennifer Mesureur, Joana R Feliciano, Nelly Wagner, Margarida C Gomes, Lili Zhang, Monica Blanco-Gonzalez, Michiel van der Vaart, David O'Callaghan, Annemarie H Meijer, Annette C Vergunst
Bacteria of the Burkholderia cepacia complex (Bcc) can cause devastating pulmonary infections in cystic fibrosis (CF) patients, yet the precise mechanisms underlying inflammation, recurrent exacerbations and transition from chronic stages to acute infection and septicemia are not known. Bcc bacteria are generally believed to have a predominant extracellular biofilm life style in infected CF lungs, similar to Pseudomonas aeruginosa, but this has been challenged by clinical observations which show Bcc bacteria predominantly in macrophages...
June 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28643413/effects-of-sodium-chloride-on-heat-resistance-oxidative-susceptibility-motility-biofilm-and-plaque-formation-of-burkholderia-pseudomallei
#4
Pornpan Pumirat, Muthita Vanaporn, Usa Boonyuen, Nitaya Indrawattana, Amporn Rungruengkitkun, Narisara Chantratita
Burkholderia pseudomallei is an environmental saprophyte and the causative agent of melioidosis, a severe infectious disease prevalent in tropical areas, including southeast Asia and northern Australia. In Thailand, the highest incidence of melioidosis is in the northeast region, where saline soil and water are abundant. We hypothesized that B. pseudomallei develops an ability to thrive in saline conditions and gains a selective ecological advantage over other soil-dwelling microorganisms. However, little is known about how an elevated NaCl concentration affects survival and adaptive changes in this pathogen...
June 23, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28593045/virulence-traits-associated-with-burkholderia-cenocepacia-st856-epidemic-strain-isolated-from-cystic-fibrosis-patients
#5
Milka Malešević, Zorica Vasiljević, Aleksandar Sovtić, Brankica Filipić, Katarina Novović, Milan Kojić, Branko Jovčić
BACKGROUND: Burkholderia cenocepacia is considered one of the most problematic cystic fibrosis (CF) pathogens. Colonization prevalence in the Serbian CF population is high and virtually exclusively limited to a single highly transmissible clone of B. cenocepacia ST856 which is positive for both the B. cepacia epidemic strain marker (BCESM) and cable pilin, and is closely related to the epidemic strain CZ1 (ST32). METHODS: Biofilm formation for 182 isolates, and adhesion to components of the host extracellular matrix, proteolytic activity, mucoidy and motility of selected ST856 representatives, as well as B...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28468829/fluorescence-and-nmr-spectroscopy-together-with-molecular-simulations-reveal-amphiphilic-characteristics-of-a-burkholderia-biofilm-exopolysaccharide
#6
Michelle M Kuttel, Paola Cescutti, Marco Distefano, Roberto Rizzo
Biofilms are a collective mode of bacterial life in which a self-produced matrix confines cells in close proximity to each other. Biofilms confer many advantages, including protection from chemicals (including antibiotics), entrapment of useful extracellular enzymes and nutrients, as well as opportunities for efficient recycling of molecules from dead cells. Biofilm matrices are aqueous gel-like structures composed of polysaccharides, proteins, and DNA stabilized by intermolecular interactions that may include non-polar connections...
June 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28463102/high-intracellular-c-di-gmp-levels-antagonize-quorum-sensing-and-virulence-gene-expression-in-burkholderia-cenocepacia-h111
#7
Nadine Schmid, Angela Suppiger, Elisabeth Steiner, Gabriella Pessi, Volkhard Kaever, Mustafa Fazli, Tim Tolker-Nielsen, Urs Jenal, Leo Eberl
The opportunistic human pathogen Burkholderia cenocepacia H111 uses two chemically distinct signal molecules for controlling gene expression in a cell density-dependent manner: N-acyl-homoserine lactones (AHLs) and cis-2-dodecenoic acid (BDSF). Binding of BDSF to its cognate receptor RpfR lowers the intracellular c-di-GMP level, which in turn leads to differential expression of target genes. In this study we analysed the transcriptional profile of B. cenocepacia H111 upon artificially altering the cellular c-di-GMP level...
May 3, 2017: Microbiology
https://www.readbyqxmd.com/read/28419759/regulation-of-burkholderia%C3%A2-cenocepacia-biofilm-formation-by-rpon-and-the-c-di-gmp-effector-berb
#8
Mustafa Fazli, Morten Rybtke, Elisabeth Steiner, Elisabeth Weidel, Jens Berthelsen, Julie Groizeleau, Wu Bin, Boo Zhao Zhi, Zhang Yaming, Volkhard Kaever, Michael Givskov, Rolf W Hartmann, Leo Eberl, Tim Tolker-Nielsen
Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilizing exopolysaccharide...
April 16, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28407432/diagnosis-of-biofilm-infections-in-cystic-fibrosis-patients
#9
REVIEW
Niels Høiby, Thomas Bjarnsholt, Claus Moser, Peter Østrup Jensen, Mette Kolpen, Tavs Qvist, Kasper Aanaes, Tanja Pressler, Marianne Skov, Oana Ciofu
Chronic Pseudomonas aeruginosa biofilm lung infection in cystic fibrosis patients is the best described biofilm infection in medicine. The initial focus can be the paranasal sinuses and then follows repeated colonization and infection of the lungs by aspiration. The matrix of the biofilms is dominated by alginate and the pathogenesis of tissue damage is immune complex-mediated chronic inflammation dominated by polymorphonuclear leukocytes and their products (DNA, oxygen radicals and proteases). The P. aeruginosa biofilm infection can be diagnosed by microscopy of lung tissue, sputum and mucus from the paranasal sinuses, where aggregates of the bacteria are found surrounded by the abundant alginate matrix...
April 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28406615/redox-sensitive-marr-homologue-bifr-from-burkholderia-thailandensis-regulates-biofilm-formation
#10
Ashish Gupta, Stanley M Fuentes, Anne Grove
Biofilm formation by pathogenic Burkholderia species is a serious complication as it renders the bacteria resistant to antibiotics and host defenses. Using B. thailandensis, we report here a novel redox-sensitive member of the multiple antibiotic resistance regulator (MarR) protein family, BifR, which represses biofilm formation. BifR is encoded as part of the emrB-bifR operon; emrB-bifR is divergent to ecsC, which encodes a putative LasA protease. In Pseudomonas aeruginosa, LasA has been implicated in virulence by contributing to cleavage of elastase...
May 2, 2017: Biochemistry
https://www.readbyqxmd.com/read/28325850/phenotypic-diversity-and-genotypic-flexibility-of-burkholderia-cenocepacia-during-long-term-chronic-infection-of-cystic-fibrosis-lungs
#11
Amy Huei-Yi Lee, Stephane Flibotte, Sunita Sinha, Adrianna Paiero, Rachel L Ehrlich, Sergey Balashov, Garth D Ehrlich, James E A Zlosnik, Joshua Chang Mell, Corey Nislow
Chronic bacterial infections of the lung are the leading cause of morbidity and mortality in cystic fibrosis patients. Tracking bacterial evolution during chronic infections can provide insights into how host selection pressures-including immune responses and therapeutic interventions-shape bacterial genomes. We carried out genomic and phenotypic analyses of 215 serially collected Burkholderia cenocepacia isolates from 16 cystic fibrosis patients, spanning a period of 2-20 yr and a broad range of epidemic lineages...
April 2017: Genome Research
https://www.readbyqxmd.com/read/28320949/discovery-of-scmr-as-a-global-regulator-of-secondary-metabolism-and-virulence-in-burkholderia-thailandensis-e264
#12
Dainan Mao, Leah B Bushin, Kyuho Moon, Yihan Wu, Mohammad R Seyedsayamdost
Bacteria produce a diverse array of secondary metabolites that have been invaluable in the clinic and in research. These metabolites are synthesized by dedicated biosynthetic gene clusters (BGCs), which assemble architecturally complex molecules from simple building blocks. The majority of BGCs in a given bacterium are not expressed under normal laboratory growth conditions, and our understanding of how they are silenced is in its infancy. Here, we have addressed this question in the Gram-negative model bacterium Burkholderia thailandensis E264 using genetic, transcriptomic, metabolomic, and chemical approaches...
April 4, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28301920/burkholderia-tropica-as-a-potential-microalgal-growth-promoting-bacterium-in-the-biosorption-of-mercury-from-aqueous-solutions
#13
Ana ZÁrate, July Florez, Edgardo Angulo, Lourdes Varela-Prieto, Cherlys Infante, Fredy Barrios, Beatriz Barraza, D I Gallardo, Jorge Valdés
The use of microalgal biomass is an interesting technology for the removal of heavy metals from aqueous solutions owing to its high metal-binding capacity, but the interactions with bacteria as a strategy for the removal of toxic metals have been poorly studied. The goal of the current research was to investigate the potential of Burkholderia tropica co-immobilized with Chlorella sp. in polyurethane discs for the biosorption of Hg(II) from aqueous solutions and to evaluate the influence of different Hg(II) concentrations (0...
June 28, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28296935/peptide-modification-results-in-the-formation-of-a-dimer-with-a-60-fold-enhanced-antimicrobial-activity
#14
Amal Thamri, Myriam Létourneau, Alex Djoboulian, David Chatenet, Eric Déziel, Annie Castonguay, Jonathan Perreault
Cationic antimicrobial peptides (CAMPs) occur naturally in numerous organisms and are considered as a class of antibiotics with promising potential against multi-resistant bacteria. Herein, we report a strategy that can lead to the discovery of novel small CAMPs with greatly enhanced antimicrobial activity and retained antibiofilm potential. We geared our efforts towards i) the N-terminal cysteine functionalization of a previously reported small synthetic cationic peptide (peptide 1037, KRFRIRVRV-NH2), ii) its dimerization through a disulfide bond, and iii) a preliminary antimicrobial activity assessment of the newly prepared dimer against Pseudomonas aeruginosa and Burkholderia cenocepacia, pathogens responsible for the formation of biofilms in lungs of individuals with cystic fibrosis...
2017: PloS One
https://www.readbyqxmd.com/read/28264910/expanding-role-of-type-ii-secretion-in-bacterial-pathogenesis-and-beyond
#15
REVIEW
Nicholas P Cianciotto, Richard C White
Type II secretion (T2S) is one means by which Gram-negative pathogens secrete proteins into the extracellular milieu and/or host organisms. Based upon recent genome sequencing, it is clear that T2S is largely restricted to the Proteobacteria, occurring in many, but not all, genera in the Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, and Deltaproteobacteria classes. Prominent human and/or animal pathogens that express a T2S system(s) include Acinetobacter baumannii, Burkholderia pseudomallei, Chlamydia trachomatis, Escherichia coli, Klebsiella pneumoniae, Legionella pneumophila, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Vibrio cholerae, and Yersinia enterocolitica T2S-expressing plant pathogens include Dickeya dadantii, Erwinia amylovora, Pectobacterium carotovorum, Ralstonia solanacearum, Xanthomonas campestris, Xanthomonas oryzae, and Xylella fastidiosa T2S also occurs in nonpathogenic bacteria, facilitating symbioses, among other things...
May 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28248999/evaluation-of-combination-therapy-for-burkholderia-cenocepacia-lung-infection-in-different-in-vitro-and-in-vivo-models
#16
Freija Van den Driessche, Bieke Vanhoutte, Gilles Brackman, Aurélie Crabbé, Petra Rigole, Jurgen Vercruysse, Glenn Verstraete, Davie Cappoen, Chris Vervaet, Paul Cos, Tom Coenye
Burkholderia cenocepacia is an opportunistic pathogen responsible for life-threatening infections in cystic fibrosis patients. B. cenocepacia is extremely resistant towards antibiotics and therapy is complicated by its ability to form biofilms. We investigated the efficacy of an alternative antimicrobial strategy for B. cenocepacia lung infections using in vitro and in vivo models. A screening of the NIH Clinical Collection 1&2 was performed against B. cenocepacia biofilms formed in 96-well microtiter plates in the presence of tobramycin to identify repurposing candidates with potentiator activity...
2017: PloS One
https://www.readbyqxmd.com/read/28124968/-accurate-diagnosis-of-pseudomonas-luteola-in-routine-microbiology-laboratory-on-the-occasion-of-two-isolates
#17
Muharrem Çiçek, Gülşen Hasçelik, H Kaan Müştak, K Serdar Diker, Burçin Şener
Pseudomonas luteola which was previously known as Chryseomonas luteola; is a gram-negative, non-fermentative, aerobic, motile, non-spore-forming bacillus. It is frequently found as a saprophyte in soil, water and other damp environments and is an opportunistic pathogen in patients with underlying medical disorders or with indwelling catheters. It has been reported as an uncommon cause of bacteremia, sepsis, septic arthritis, meningitis, endocarditis, and peritonitis. Thus, early and accurate identification of this rare species is important for the treatment and also to provide information about the epidemiology of P...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28120710/the-role-of-universal-stress-proteins-in-bacterial-infections
#18
Andrew O Connor, Siobhán McClean
Universal stress proteins are ubiquitously expressed in bacteria, archaea and plants and other eukaryotes. A general property of USPs is their role in adaptation of bacteria to oxidative stress, high temperature, low pH and/or hypoxia. There is increasing evidence that these proteins facilitate the adaption of bacterial pathogens to the human host environment, thereby facilitating colonisation and pathogenicity. USPs in Mycobacterium tuberculosis are well studied and may play a role in latency of tuberculosis...
January 24, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28072820/p-cymene-promotes-its-catabolism-through-the-p-cymene-and-the-p-cumate-pathways-activates-a-stress-response-and-reduces-the-biofilm-formation-in-burkholderia-xenovorans-lb400
#19
Loreine Agulló, María José Romero-Silva, Mirian Domenech, Michael Seeger
p-Cymene is an aromatic terpene that is present in diverse plant species. The aims of this study were to study the p-cymene metabolism in the model aromatic-degrading bacterium Burkholderia xenovorans LB400, and its response to p-cymene. The catabolic p-cymene (cym) and p-cumate (cmt) genes are clustered on the LB400 major chromosome. B. xenovorans LB400 was able to grow on p-cymene as well as on p-cumate as a sole carbon and energy sources. LB400 growth attained higher cell concentration at stationary phase on p-cumate than on p-cymene...
2017: PloS One
https://www.readbyqxmd.com/read/28065676/rhamnolipids-from-non-pathogenic-burkholderia-thailandensis-e264-physicochemical-characterization-antimicrobial-and-antibiofilm-efficacy-against-oral-hygiene-related-pathogens
#20
Mohamed Elshikh, Scott Funston, Alif Chebbi, Syed Ahmed, Roger Marchant, Ibrahim M Banat
Biosurfactants are naturally occurring surface active compounds that have mainly been exploited for environmental applications and consumer products, with their biomedical efficacy an emerging area of research. Rhamnolipids area major group of biosurfactants that have been reported for their antimicrobial and antibiofilm efficacy. One of the main limiting factors for scaled up production and downstream applications of rhamnolipids is the fact that they are predominantly produced from the opportunistic pathogen Pseudomonas aeruginosa...
May 25, 2017: New Biotechnology
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