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https://www.readbyqxmd.com/read/28512098/a-rare-case-of-thrombotic-microangiopathy-triggered-by-acute-pancreatitis
#1
Kevin Singh, Ahmed Jamal Nadeem, Behzad Doratotaj
Thrombotic microangiopathy (TMA) occurring after acute pancreatitis is rarely described. Without prompt intervention, TMA can be, and often is, lethal, so prompt recognition is important. Here, we present a case of a 61-year-old woman with a history of alcohol misuse who presented with epigastric pain, nausea and vomiting after binge drinking. Elevated serum lipase and imaging were suggestive of acute-on-chronic pancreatitis. Although the patient's symptoms of acute pancreatitis subsided, her anaemia, thrombocytopenia and acute kidney injury worsened...
May 15, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28488138/enantioselective-resolution-of-racemic-flurbiprofen-methyl-ester-by-lipase-encapsulated-mercapto-calix-4-arenes-capped-fe3o4-nanoparticles
#2
Hasan Yildiz, Elif Ozyilmaz, Asif Ali Bhatti, Mustafa Yilmaz
This work presents the synthesis of new mercapto calix[4]arenes derivatives (4 and 5). These derivatives were capped on Fe3O4 magnetic nanoparticles and subsequently encapsulated with Candida rugosa through sol-gel method to furnish enc-4 and enc-5, respectively, to enhance catalytic activity and enantioselectivity of lipase for hydrolysis reaction of racemic flurbiprofen methyl ester. Catalytic activity and enantioselectivity of enc-4 and enc-5 were assayed at different pH and temperature conditions and it was found that the resultant encapsulated enzyme exhibited higher thermal and operational stabilities compared to the free lipase in which enc-5 showed the excellent rate of enantioselectivity (E = 176) for S-flurbiprofen better than free lipase (E = 137) at pH 7 and 35 °C for 48 h...
May 9, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28480147/effect-of-the-molecular-weight-of-water-soluble-chitosan-on-its-fat-cholesterol-binding-capacities-and-inhibitory-activities-to-pancreatic-lipase
#3
Qiu Jin, Huahua Yu, Xueqin Wang, Kecheng Li, Pengcheng Li
BACKGROUND: Obesity has become a worldwide burden to public health in recent decades. Given that obesity is caused by an imbalance between caloric intake and expenditure, and that dietary fat is the most important energy source of all macronutrients (by providing the most calories), a valuable strategy for obesity treatment and prevention is to block fat absorption via the gastrointestinal pathway. In this study, the fat- and cholesterol-binding capacities and the inhibition of pancreatic lipase by water-soluble chitosan (WSC) with different weight-average molecular weight (Mw) were tested and compared in vitro, in order to determine the anti-obesity effects of WSC and the influence of its Mw...
2017: PeerJ
https://www.readbyqxmd.com/read/28479718/potential-lipid-lowering-effects-of-eleusine-indica-l-gaertn-extract-on-high-fat-diet-induced-hyperlipidemic-rats
#4
Siew Ling Ong, Koteswara Rao Nalamolu, How Yee Lai
BACKGROUND: To date, anti-obesity agents based on natural products are tested for their potential using lipase inhibition assay through the interference of hydrolysis of fat by lipase resulting in reduced fat absorption without altering the central mechanisms. Previous screening study had indicated strong anti-obesity potential in Eleusine indica (E. indica), but to date, no pharmacologic studies have been reported so far. OBJECTIVE: This study was performed to investigate the lipid-lowering effects of E...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28465058/establishment-and-application-of-a-modified-membrane-blot-assay-for-rhizomucor-miehei-lipases-aimed-at-improving-their-methanol-tolerance-and-thermostability
#5
Dong He, Wen Luo, Zhiyuan Wang, Pengmei Lv, Zhenhong Yuan, Shaowei Huang, Jingliang Xv
Directed evolution has been proved an effective way to improve the stability of proteins, but high throughput screening assays for directed evolution with simultaneous improvement of two or more properties are still rare. In this study, we aimed to establish a membrane-blot assay for use in the high-throughput screening of Rhizomucor miehei lipases (RMLs). With the assistance of the membrane-blot screening assay, a mutant E47K named G10 that showed improved thermal stability was detected in the first round of error-prone PCR...
July 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28459005/discovery-and-characterizaton-of-a-novel-lipase-with-transesterification-activity-from-hot-spring-metagenomic-library
#6
Wei Yan, Furong Li, Li Wang, Yaxin Zhu, Zhiyang Dong, Linhan Bai
A new gene encoding a lipase (designated as Lip-1) was identified from a metagenomic bacterial artificial chromosome(BAC) library prepared from a concentrated water sample collected from a hot spring field in Niujie, Eryuan of Yunnan province in China. The open reading frame of this gene encoded 622 amino acid residues. It was cloned, fused with the oleosin gene and over expressed in Escherichia coli to prepare immobilized lipase artificial oil body AOB-sole-lip-1. The monomeric Sole-lip-1 fusion protein presented a molecular mass of 102...
March 2017: Biotechnology Reports
https://www.readbyqxmd.com/read/28448467/chemical-composition-and-biological-activities-of-mono-and-heterofloral-bee-pollen-of-different-geographical-origins
#7
Jucilene Silva Araújo, Emerson Dechechi Chambó, Maria Angélica Pereira de Carvalho Costa, Samira Maria Peixoto Cavalcante da Silva, Carlos Alfredo Lopes de Carvalho, Leticia M Estevinho
Recent research shows variations in pollen chemical constituents and, consequently, in their therapeutic properties. Mono and multifloral bee pollen extracts were investigated for antioxidant and enzyme inhibitory activity properties, phenolic compounds and fatty acid composition. Generally, Eucalyptus spp. and multifloral extracts exhibited potent inhibitory activity against α-amylase, acetylcholinesterase, tyrosinase, lipoxygenase, lipase and hyaluronidase. On the other hand, Miconia spp. demonstrated higher antihemolytic activity...
April 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28447802/potential-lipid-lowering-mechanisms-of-biochanin-a
#8
Zhaohui Xue, Qian Zhang, Wancong Yu, Haichao Wen, Xiaonan Hou, Dan Li, Xiaohong Kou
Extensive studies have demonstrated that biochanin A (BCA) has a significant hypolipidemic effect. However, its mechanism of action is not clear. In this context, the effect of BCA on a high-fat diet (HFD)-induced hyperlipidemia in mice was determined. The results showed that treatment with a medium dose of biochanin A (BM) significantly decreased low-density lipoprotein cholesterol (LDL-C) 85% (from 1.196 ± 0.183 to 0.181 ± 0.0778 mM) and total cholesterol (TC) 39% (from 5.983 ± 0.128 to 3.649 ± 0.374 mM) levels, increased lipoprotein lipase (LPL) 96% (from 1...
May 4, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28444107/apelin-13-inhibits-lipoprotein-lipase-expression-via-the-apj-pkc%C3%AE-mir-361-5p-signaling-pathway-in-thp-1-macrophage-derived-foam-cells
#9
Xin Zhang, Qiong Ye, Duo Gong, Yuan Lv, Haipeng Cheng, Chong Huang, Lingyan Chen, Zhenwang Zhao, Liang Li, Xie Wei, Min Zhang, Xiaodan Xia, Xiaohua Yu, Xilong Zheng, Shuzhi Wang, Zongbao Wang, Chaoke Tang
Atherosclerotic lesions are characterized by the accumulation of abundant lipids and chronic inflammation. Previous researches have indicated that macrophage-derived lipoprotein lipase (LPL) promotes atherosclerosis progression by accelerating lipid accumulation and pro-inflammatory cytokine secretion. Although apelin-13 has been regarded as an atheroprotective factor, it remains unclear whether it can regulate the expression of LPL. The aim of this study was to explore the effects of apelin-13 on the expression of LPL and the underlying mechanism in THP-1 macrophage-derived foam cells...
April 21, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28441610/total-esterase-measurement-in-saliva-of-pigs-validation-of-an-automated-assay-characterization-and-changes-in-stress-and-disease-conditions
#10
Fernando Tecles, María D Contreras-Aguilar, Silvia Martínez-Miró, Asta Tvarijonaviciute, Silvia Martínez-Subiela, Damián Escribano, José J Cerón
An automated spectrophotometric method for total esterase activity (TEA) measurement in porcine saliva has been developed and validated, using 4-nitrophenyl acetate (4-NA) as substrate. The method was precise and accurate, with low limit of detection, and was able to measure samples with TEA activities up to 400IU/L without any dilution. In addition, the different enzymes contributing to TEA were characterized, being identified carbonic anhydrase VI (CA-VI), lipase, cholinesterase (ChE) and cholesterol esterase (CEL)...
April 18, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28431976/the-impact-of-exposure-to-environmental-contaminant-on-hepatocellular-lipid-metabolism
#11
Zongguang Hui, Rujiang Li, Li Chen
Increasing evidences show that ubiquitous perfluorooctanoic acid (PFOA), a representative environmental pollutant, is found to be linked to lipid dysmetabolism. However, the biological mechanism behind this outcome remains uninvestigated. In the present study, we established the PFOA-injured liver in mice to explore the underlying mechanism associated with PFOA-induced lipid disturbance in the liver via a group of biochemical and molecular assays. As results, PFOA-exposed mice showed increased transaminase (ALT), reduced triglyceride and free fatty acid contents in serum, as well as elevated level of hepatic triglyceride...
April 18, 2017: Gene
https://www.readbyqxmd.com/read/28428356/biological-variation-estimates-obtained-from-91-healthy-study-participants-for-9-enzymes-in-serum
#12
Anna Carobene, Thomas Røraas, Una Ørvim Sølvik, Marit Sverresdotter Sylte, Sverre Sandberg, Elena Guerra, Irene Marino, Niels Jonker, Gerhard Barla, William A Bartlett, Pilar Fernandez-Calle, Jorge Díaz-Garzón, Francesca Tosato, Mario Plebani, Abdurrahman Coŝkun, Mustafa Serteser, Ibrahim Unsal, Ferruccio Ceriotti
BACKGROUND: We sought to develop estimates of biological variation (BV) for 9 enzymes in blood serum as part of the European Biological Variation Study. METHODS: Ninety-one healthy study participants (38 male and 53 female, 21-69 years old) were phlebotomized in each of 10 consecutive weeks at 6 European laboratories. The same preanalytical sample-handling protocol was followed at each center before transport to San Raffaele Hospital, Milan, Italy, for analysis...
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28425950/diversity-analysis-and-bioresource-characterization-of-halophilic-bacteria-isolated-from-a-south-african-saltpan
#13
Ramganesh Selvarajan, Timothy Sibanda, Memory Tekere, Hlengilizwe Nyoni, Stephen Meddows-Taylor
Though intensive research has been channeled towards the biotechnological applications of halophiles and other extremophilic microbes, these studies have not been, by any means, exhaustive. Saline environments still offer a vast diversity of microbes with potential to produce an array of natural products which can only be unlocked by concerted research efforts. In this study, a combination of culture and molecular approaches were employed to characterize halophilic bacteria from saltpan water samples and profile their potential biotechnological applications...
April 20, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28415797/rab7-gtpase-controls-lipid-metabolic-signaling-in-myeloid-derived-suppressor-cells
#14
Xinchun Ding, Wenjing Zhang, Ting Zhao, Cong Yan, Hong Du
Lysosomal acid lipase (LAL) is a critical neutral lipid metabolic enzyme that regulates metabolic reprogramming in myeloid-derived suppressor cells (MDSCs) through over-activation of mammalian target of rapamycin (mTOR). Affymetrix GeneChip microarray analysis of MDSCs from LAL deficient mouse (lal-/-) revealed upregulation of Rab7 GTPase protein, which belongs to a superfamily of small-molecular-weight GTPase known to regulate intracellular membrane trafficking from early to late endosomes and lysosomes. Here, the physical protein-protein interaction between Rab7 GTPase and mTOR has been detected by co-immunoprecipitation in the cell extract of wild type HD1A and lal-/- MDSC-like HD1B myeloid cell lines...
May 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28386657/study-on-the-morphology-histology-and-enzymatic-activity-of-the-digestive-tract-of-gymnocypris-eckloni-herzenstein
#15
Lizhu Yang, Jing Fang, Xi Peng, Hengmin Cui, Min He, Zhicai Zuo, Yi Zhou, Zhuangzhi Yang
The present research was conducted to study the morphology, histology and enzymatic activities of the digestive tract of Gymnocypris eckloni by light and transmission electron microscopes as well as by enzyme assays. The digestive tract of G. eckloni consisted of the oropharyngeal cavity, oesophagus and intestine. The wall of the digestive tract was composed of mucosa, submucosa, muscularis and serosa but lacked muscularis mucosa and glands. The stratified epithelium of the oropharyngeal cavity and oesophagus contained numerous mucous cells...
April 7, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28359787/tips-on-the-analysis-of-phosphatidic-acid-by-the-fluorometric-coupled-enzyme-assay
#16
Azam Hassaninasab, Gil-Soo Han, George M Carman
The fluorometric coupled enzyme assay to measure phosphatidic acid (PA) involves the solubilization of extracted lipids in Triton X-100, deacylation, and the oxidation of PA-derived glycerol-3-phosphate to produce hydrogen peroxide for conversion of Amplex Red to resorufin. The enzyme assay is sensitive, but plagued by high background fluorescence from the peroxide-containing detergent and incomplete heat inactivation of lipoprotein lipase. These problems affecting the assay reproducibility were obviated by the use of highly pure Triton X-100 and by sufficient heat inactivation of the lipase enzyme...
June 1, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28317614/snap-tests-for-pancreatitis-in-dogs-and-cats-snap-canine-pancreatic-lipase-and-snap-feline-pancreatic-lipase
#17
REVIEW
Panagiotis G Xenoulis, Jörg M Steiner
A clinical diagnosis of pancreatitis in dogs and cats can be challenging. Several diagnostic modalities have been evaluated over the years for the diagnosis of canine and feline pancreatitis, but most of these modalities have been shown to be of limited clinical use because of poor performance, limited availability, or because they are invasive, or all of these. Assays for the measurement of pancreatic lipase (PL) immunoreactivity [Specific canine PL (Spec cPL) in dogs and Specific feline PL (Spec fPL) in cats] were first developed approximately 15 years ago, and studies have shown that they are currently the serum tests of choice for the evaluation of canine and feline patients, respectively, suspected of having pancreatitis...
December 2016: Topics in Companion Animal Medicine
https://www.readbyqxmd.com/read/28315735/very-rapid-amyloid-fibril-formation-by-a-bacterial-lipase-in-the-absence-of-a-detectable-lag-phase
#18
Fatemeh Rashno, Khosro Khajeh, Claudia Capitini, Reza H Sajedi, Maryam Monsef Shokri, Fabrizio Chiti
The conversion of proteins from their soluble states into well-organized amyloid fibrils has received abundant attention. This process typically consists of three stages: lag, growth and plateau phases. In this study, the process of amyloid fibril formation by lipase from Pseudomonas sp. after diluting out urea was examined by Thioflavin T (ThT) fluorescence, Congo red (CR) binding, 8-anilinonaphthalene-1-sulfonic acid (ANS) binding, dynamic light scattering (DLS), circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopies, X-ray diffraction (XRD) and transmission electron microscopy (TEM)...
June 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28315508/tumor-markers-in-pancreatic-cystic-fluids-for-diagnosis-of-malignant-cysts
#19
Alexandre Levy, Theodora Popovici, Phuong-Nhi Bories
BACKGROUND AND AIM: Early diagnosis of premalignant or malignant pancreatic cysts is essential to improve prognosis. Sampling of pancreatic cyst fluid by fine-needle aspiration during endoscopic ultrasonography (EUS) enables cytopathological examination combined with biochemical analysis. This study aimed to provide an aid based on biological markers for the preoperative management of patients with pancreatic cysts. METHODS: Pancreatic fluids obtained by EUS-guided fine-needle aspiration from 115 patients with cystic lesions were assayed for amylase, lipase, carcinoembryonic antigen (CEA), CA 19-9 and CA 72-4...
March 3, 2017: International Journal of Biological Markers
https://www.readbyqxmd.com/read/28287485/chemical-constituents-of-smilax-china-l-stems-and-their-inhibitory-activities-against-glycation-aldose-reductase-%C3%AE-glucosidase-and-lipase
#20
Hee Eun Lee, Jin Ah Kim, Wan Kyunn Whang
The search for natural inhibitors with anti-diabetes properties has gained increasing attention. Among four selected Smilacaceae family plants, Smilax china L. stems (SCS) showed significant in vitro anti-glycation and rat lens aldose reductase inhibitory activities. Bioactivity-guided isolation was performed with SCS and four solvent fractions were obtained, which in turn yielded 10 compounds, including one phenolic acid, three chlorogenic acids, four flavonoids, one stilbene, and one phenylpropanoid glycoside; their structures were elucidated using nuclear magnetic resonance and mass spectrometry...
March 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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