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https://www.readbyqxmd.com/read/29433298/chemical-composition-and-inhibitory-activities-on-dipeptidyl-peptidase-iv-and-pancreatic-lipase-of-two-underutilized-species-from-the-brazilian-savannah-oxalis-cordata-a-st-hil-and-xylopia-aromatica-lam-mart
#1
Verena B Oliveira, Raquel L B Araújo, Thomas Eidenberger, Maria G L Brandão
Brazil has the greatest vegetal biodiversity in the world, but products derived from native species are not optimally utilized. Oxalis cordata and Xylopia aromatica are two underutilized species whose leaves and fruits, respectively, have been used as food in the 19th century. In this study, we used chemical and in vitro assays to evaluate the potential of these species as functional foods. The inhibitory activity on pancreatic lipase and DPP-IV were evaluated using the crude extracts and fractions ethyl acetate, butanol and water of these two species...
March 2018: Food Research International
https://www.readbyqxmd.com/read/29433189/changes-in-polyphenol-composition-and-bioactivity-of-the-native-chilean-white-strawberry-fragaria-chiloensis-spp-chiloensis-f-chiloensis-after-in-vitro-gastrointestinal-digestion
#2
Samanta Thomas-Valdés, Cristina Theoduloz, Felipe Jiménez-Aspee, Alberto Burgos-Edwards, Guillermo Schmeda-Hirschmann
The Chilean white strawberry (Fragaria chiloensis spp. chiloensis f. chiloensis) is a semi-domesticated strawberry with high polyphenol content and antioxidant activity occurring in southern Chile. The aim of this work was to compare the composition and bioactivity of the polyphenol-enriched fruit extract (PEE) before and after simulated gastrointestinal digestion (GID). Results show a decrease by >50% in the total phenolic (TP) content at the end of the GID, compared to the non-digested PEE. A reduction in the antioxidant capacity of the PEEs was observed after GID by means of DPPH, FRAP, TEAC and anion superoxide assays...
March 2018: Food Research International
https://www.readbyqxmd.com/read/29430365/bioactive-compounds-from-mangrove-derived-rare-actinobacterium-saccharomonospora-oceani-vjds-3
#3
Manideepa Indupalli, Vijayalakshmi Muvva, Ushakiranmayi Mangamuri, Rajesh Kumar Munaganti, Krishna Naragani
A rare actinobacterium was isolated from Nizampatnam mangrove ecosystem of Andhra Pradesh, India, and was screened for its ability to produce bioactive compounds. The potential strain was identified as Saccharomonospora oceani VJDS-3 by polyphasic taxonomy. Purification of the biologically active compounds by column chromatography led to the isolation of three compounds, namely methoxy ethyl cinnamate (ethyl(E)-3-(4-methoxyphenyl)acrylate) (R1), 4-hydroxy methyl cinnamate (methyl(E)-3-(4-hydroxyphenyl)acrylate) (R2) and 4-methylbenzoic acid (R3)...
February 2018: 3 Biotech
https://www.readbyqxmd.com/read/29425747/annona-muricata-linn-leaf-as-a-source-of-antioxidant-compounds-with-in-vitro-antidiabetic-and-inhibitory-potential-against-%C3%AE-amylase-%C3%AE-glucosidase-lipase-non-enzymatic-glycation-and-lipid-peroxidation
#4
Allisson Benatti Justino, Natália Carnevalli Miranda, Rodrigo Rodrigues Franco, Mário Machado Martins, Neide Maria da Silva, Foued Salmen Espindola
Annona muricata leaves are used in traditional medicine to manage diabetes mellitus and its complications. The aim of this study was to evaluate the potential in vitro antidiabetic properties of Annona muricata leaf by identifying its main phytochemical constituents and characterizing the phenolic-enriched fractions for their in vitro antioxidant capacity and inhibitory activities against glycoside and lipid hydrolases, advanced glycation end-product formation and lipid peroxidation. Ethanol extract of A. muricata leaf was subjected to a liquid-liquid partitioning and its fractions were used in enzymatic assays to evaluate their inhibitory potential against α-amylase, α-glucosidase and lipase, as well as their antioxidant (DPPH, ORAC, FRAP and Fe2+-ascorbate-induced lipid peroxidation assays) and anti-glycation (BSA-fructose, BSA-methylglyoxal and arginine-methylglyoxal models) capacities...
February 6, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29424454/evaluation-of-snap-cpl-spec-cpl-vetscan-cpl-rapid-test-and-precision-psl-assays-for-the-diagnosis-of-clinical-pancreatitis-in-dogs
#5
H Cridge, A G MacLeod, G E Pachtinger, A J Mackin, A M Sullivant, J M Thomason, T M Archer, K V Lunsford, K Rosenthal, R W Wills
BACKGROUND: The sensitivity, specificity, and agreement of 4 diagnostic assays (SNAP canine pancreatic lipase (cPL), specific cPL (Spec cPL), VetScan cPL Rapid Test, and Precision PSL) for pancreatitis in dogs have not been directly compared. HYPOTHESIS/OBJECTIVES: To determine the level of agreement among each of the 4 assays and a clinical suspicion score, level of agreement among the assays, and sensitivity and specificity of each assay in a clinically relevant patient group...
February 9, 2018: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29417328/identification-of-small-proteins-and-peptides-in-the-differentiation-of-patients-with-intraductal-mucinous-neoplasms-of-the-pancreas-chronic-pancreatitis-and-pancreatic-adenocarcinoma
#6
Chiara Fania, Raffaele Pezzilli, Gianvico Melzi d'Eril, Cecilia Gelfi, Alessandra Barassi
BACKGROUND: There are a limited number of studies investigating the type of serum proteins capable of differentiating intraductal papillary mucinous neoplasms from benign or malignant diseases of the pancreas. AIMS: To select proteins able to differentiate intraductal papillary mucinous neoplasms from benign and malignant pancreatic disease using semiquantitative proteomics. METHODS: Serum samples were obtained from 74 patients (19 with type II intraductal papillary mucinous neoplasms, 8 with type I/III intraductal papillary mucinous neoplasms, 24 with chronic pancreatitis, 23 with pancreatic ductal adenocarcinomas) and 21 healthy subjects...
February 8, 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29414121/sacred-maya-incense-copal-protium-copal-burseraceae-has-antianxiety-effects-in-animal-models
#7
Zul Merali, Christian Cayer, Pamela Kent, Rui Liu, Victor Cal, Cory S Harris, John T Arnason
ETHNOPHARMACOLOGICAL RELEVANCE: The Maya have traditionally used copal, Protium copal, as incense during ceremonies since pre-Columbian times. Anecdotally, copal (when burned as incense), is thought to elicit mentally uplifting and calming effects. The main objective of this study was to determine whether the incense elicits anxiolytic-like behavior in animal models using rats. A second objective was to characterize active constituents and discern potential mechanism(s) of action, specifically the involvement of the GABAergic and endocannabinoid (eCB) systems...
January 29, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29410257/plasticizers-used-in-food-contact-materials-affect-adipogenesis-in-3t3-l1-cells
#8
Valentina Pomatto, Erika Cottone, Paolo Cocci, Matteo Mozzicafreddo, Gilberto Mosconi, Erik Russel Nelson, Francesco Alessandro Palermo, Patrizia Bovolin
Recent studies suggest that exposure to some plasticizers, such as Bisphenol A (BPA), play a role in endocrine/metabolic dispruption and can affect lipid accumulation in adipocytes. Here, we investigated the adipogenic activity and nuclear receptor interactions of four plasticizers approved for the manufacturing of food-contact materials (FCMs) and currently considered safer alternatives. Differentiating 3T3-L1 mouse preadipocytes were exposed to scalar concentrations (0.01-25 μM) of DiNP (Di-iso-nonyl-phthalate), DiDP (Di-iso-decyl-phthalate), DEGDB (Diethylene glycol dibenzoate), or TMCP (Tri-m-cresyl phosphate)...
February 2, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29402262/effects-of-panax-notoginseng-saponins-on-severe-acute-pancreatitis-through-the-regulation-of-mtor-akt-and-caspase-3-signaling-pathway-by-upregulating-mir-181b-expression-in-rats
#9
Ming-Wei Liu, Rui Wei, Mei-Xian Su, Hui Li, Tian-Wen Fang, Wei Zhang
BACKGROUND: In China, Panax notoginseng has been used to treat oxidative stress-related diseases for a long time. Panax notoginseng saponins is an extract from Panax notoginseng Ledeb. Its therapeutic potential is related to antioxidant activity, but related mechanisms are still unclear. The study aims to assess the protection effects of Panax notoginseng saponins in the taurocholate-induced rat model of acute pancreatitis (AP) and explore underlying mechanisms. METHODS: A rat model of severe acute pancreatitis (SAP) was established in rats induced with taurocholate...
February 5, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29393354/effects-of-silencing-the-dusp1-gene-using-lentiviral-vector-mediated-sirna-on-the-release-of-proinflammatory-cytokines-through-regulation-of-the-mapk-signaling-pathway-in-mice-with-acute-pancreatitis
#10
Bo Zhang, Shu-Liang Li, Hua-Lei Xie, Jia-Wei Fan, Chuan-Wei Gu, Chao Kang, Mu-Jian Teng
The present study investigated the effects of dual specificity phosphatase 1 (DUSP1) gene silencing using lentiviral vector-mediated small interfering (si)RNA on the release of proinflammatory cytokines through the regulation of the mitogen‑activated protein kinase (MAPK) signaling pathway in mice with acute pancreatitis (AP). Two siRNA‑DUSP1 sequences and one scramble siRNA sequence were designed, and the expression of DUSP1 was detected using western blot analysis to screen for the one with a higher interference rate...
January 25, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29383887/-effect-of-eletroacupuncture-intervention-on-insulin-resistance-lipid-metabolic-disorder-and-expression-of-hepatic-srebp-1-c-and-fatty-acid-synthase-proteins-in-rats-with-hyperlipidemia
#11
Zhi-Xing Li, Hai-Hua Zhang, Dan-Chun Lan, Hong-Tao Zhang, Xiao-Zhuan Chen, Jian Sun
OBJECTIVE: To observe the effect of electroacupuncture(EA) of "Fenglong" (ST 40) and "Sanyinjiao" (SP 6) on lipid metabolic disorder, insulin resistance (IR) and expression of sterol regulatory element blinding protein-1 (SREBP-1) c and fatty acid synthase (FAS) proteins in the liver tissue in hyperlipidemia rats with IR, so as to reveal its mechanisms underlying improvement of IR. METHODS: Forty male SD rats were randomly divided into blank control, model, medication and EA groups (n=8 in each group)...
January 25, 2018: Zhen Ci Yan Jiu, Acupuncture Research
https://www.readbyqxmd.com/read/29360737/lasiodiplodia-theobromae-as-a-producer-of-biotechnologically-relevant-enzymes
#12
Carina Félix, Sofia Libório, Mariana Nunes, Rafael Félix, Ana S Duarte, Artur Alves, Ana C Esteves
Phytopathogenic fungi are known to produce several types of enzymes usually involved in plant cell wall degradation and pathogenesis. The increasing of global temperature may induce fungi, such as Lasiodiplodia theobromae (L. theobromae), to alter its behavior. Nonetheless, there is only limited information regarding the effect of temperature on L. theobromae production of enzymes. The need for new, thermostable enzymes, that are biotechnologically relevant, led us to investigate the effect of temperature on the production of several extracellular enzymatic activities by different L...
January 23, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29358478/identification-of-rare-diseases-by-screening-a-population-selected-on-the-basis-of-routine-pathology-results-the-pathfinder-project-lysosomal-acid-lipase-cholesteryl-ester-storage-disease-substudy
#13
Timothy M Reynolds, Clare Mewies, John Hamilton, Anthony S Wierzbicki
AIMS: Lysosomal acid lipase deficiency (LALD) is an autosomal recessive disorder of cholesterol ester storage associated with hepatic disease, cirrhosis and accelerated atherosclerosis. Its prevalence in the general population, patients with dyslipidaemia and raised transaminases is unclear. This study attempted to identify the prevalence of LALD from patients with abnormal results in laboratory databases. METHODS: Electronic laboratory databases were interrogated to identify from clinical biochemistry records patients with a phenotype of low high-density lipoprotein-cholesterol (≤0...
January 22, 2018: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29352424/cannabinoid-cb1-and-cb2%C3%A2-receptors-and-monoacylglycerol-lipase-gene-expression-alterations-in-the-basal-ganglia-of-patients-with-parkinson-s-disease
#14
Francisco Navarrete, M Salud García-Gutiérrez, Auxiliadora Aracil-Fernández, José L Lanciego, Jorge Manzanares
Previous studies suggest that the endocannabinoid system plays an important role in the neuropathological basis of Parkinson's disease (PD). This study was designed to detect potential alterations in the cannabinoid receptors CB1 (CB1r) and CB2 (A isoform, CB2Ar), and in monoacylglycerol lipase (MAGL) gene expression in the substantia nigra (SN) and putamen (PUT) of patients with PD. Immunohistochemical studies were performed to identify precise CB2r cellular localization in the SN of control and PD patients...
January 19, 2018: Neurotherapeutics: the Journal of the American Society for Experimental NeuroTherapeutics
https://www.readbyqxmd.com/read/29342975/gallic-acid-alleviates-hypertriglyceridemia-and-fat-accumulation-via-modulating-glycolysis-and-lipolysis-pathways-in-perirenal-adipose-tissues-of-rats-fed-a-high-fructose-diet
#15
Da-Wei Huang, Wen-Chang Chang, Heng-Jui Yang, James Swi-Bea Wu, Szu-Chuan Shen
This study investigated the ameliorative effect of gallic acid (GA) on hypertriglyceridemia and fat accumulation in perirenal adipose tissues of high-fructose diet (HFD)-induced diabetic rats. The previous results showed that orally administered GA (30 mg/kg body weight) for four weeks significantly reduced the levels of plasma glucose and triglyceride (TG) in HFD rats. GA also markedly decreased the perirenal adipose tissues weight of HFD rats in present study (p < 0.05). Western blot assay indicated that GA restored expression of insulin signaling-related proteins, such as insulin receptor (IR), protein kinase C-zeta (PKC-ζ), and glucose transporter-4 (GLUT4) in the perirenal adipose tissues of HFD rats...
January 15, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29339442/specific-substrate-for-the-assay-of-lysosomal-acid-lipase
#16
Sophia Masi, Naveen Chennamaneni, Frantisek Turecek, C Ronald Scott, Michael H Gelb
BACKGROUND: Deficiency of lysosomal acid lipase (LAL) causes Wolman disease and cholesterol ester storage disease. With the recent introduction of enzyme replacement therapy to manage LAL deficiency comes the need for a reliable assay of LAL enzymatic activity that can be applied to dried blood spots (DBS). METHODS: We prepared and tested a library of analogs of palmitoyl 4-methylumbelifferyl esters to find a highly active and specific substrate for LAL in DBS. The LAL assay was optimized leading to both LC-MS/MS and fluorometric assay of LAL...
January 16, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29336184/alpha-amylase-%C3%AE-glucosidase-and-lipase-inhibiting-activities-of-polyphenol-rich-extracts-from-six-common-bean-cultivars-of-southern-italy-before-and-after-cooking
#17
Maria Neve Ombra, Antonio d'Acierno, Filomena Nazzaro, Patrizia Spigno, Riccardo Riccardi, Massimo Zaccardelli, Catello Pane, Raffaele Coppola, Florinda Fratianni
Common beans (Phaseolus vulgaris) are a good source of nutrients and phenolic compounds with versatile health benefits. Polyphenol-rich extracts of six ecotypes of P. vulgaris were analysed to determine their phenolic profiles and assayed in vitro for inhibitory effects on digestive enzymes relevant to carbohydrates and lipids metabolism. The extracts inhibited enzyme activities in a dose-dependent manner. IC 50 values ranged from 69 ± 1.9 to 126 ± 3.2 μg/mL and from 107.01 ± 4.5 to 184.20 ± 5...
January 16, 2018: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/29322001/evaluation-of-the-antidiabetic-property-of-aqueous-leaves-extract-of%C3%A2-zanthoxylum-armatum-dc-using-in%C3%A2-vivo-and-in%C3%A2-vitro-approaches
#18
Carey Vana Rynjah, Ningthoujam Neelima Devi, Nasheman Khongthaw, Donkupar Syiem, Suktilang Majaw
The present study was designed to evaluate the antidiabetic potential of the aqueous leaves extract of Zanthoxylum armatum DC. leaves using in vivo and in vitro approaches. For in vivo studies, blood glucose level was monitored at different intervals after administration of varying doses of the extract for its hypoglycemic (100-6000 mg/kg b.w.) and antihyperglycemic (250 mg/kg b.w.) effect in normoglycemic and diabetic mice. In vitro enzymatic inhibition activity was tested against α-amylase, α- and β-glucosidase and lipase...
January 2018: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/29320799/new-phenylethanoid-glycosides-from-cistanche-phelypaea-and-their-activity-as-inhibitors-of-monoacylglycerol-lipase-magl
#19
Khadidja Aya Beladjila, Djemaa Berrehal, Nunziatina De Tommasi, Carlotta Granchi, Giulia Bononi, Alessandra Braca, Marinella De Leo
Four new phenylethanoid glycosides (1: -4: ), 1-β-p-hydroxyphenyl-ethyl-2-O-acetyl-3,6-di-α-l-rhamnopyranosyl-β-d-glucopyranoside (1: ), 1-β-p-hydroxyphenyl-ethyl-3,6-O-di-α-l-rhamnopyranosyl-β-d-glucopyranoside (2: ), 1-β-p-hydroxyphenyl-ethyl-2-O-acetyl-3,6-di-α-l-rhamnopyranosyl-4-p-coumaroyl-β-d-glucopyranoside (3: ), and 1-β-p-hydroxyphenyl-ethyl-3,6-di-α-l-rhamnopyranosyl-4-p-coumaroyl-β-d-glucopyranoside (4: ), together with three known compounds, were isolated from the n-butanol extract of Cistanche phelypaea aerial parts...
January 10, 2018: Planta Medica
https://www.readbyqxmd.com/read/29310667/wwl70-protects-against-chronic-constriction-injury-induced-neuropathic-pain-in-mice-by-cannabinoid-receptor-independent-mechanisms
#20
Jie Wen, Melissa Jones, Mikiei Tanaka, Prabhuanand Selvaraj, Aviva J Symes, Brian Cox, Yumin Zhang
BACKGROUND: Targeting the endocannabinoid system has emerged as an effective strategy for the treatment of inflammatory and neurological diseases. Unlike the inhibition of the principal 2-arachidonyl glycerol (2-AG) hydrolytic enzyme monoacylglycerol lipase (MAGL), which leads to 2-AG overload and cannabinoid receptor desensitization, selective inhibition of the minor 2-AG hydrolytic enzyme alpha, beta-hydrolase domain 6 (ABHD6) can provide therapeutic benefits without producing cannabimimetic side effects...
January 8, 2018: Journal of Neuroinflammation
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