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https://www.readbyqxmd.com/read/29031715/angptl8-promotes-the-ability-of-angptl3-to-bind-and-inhibit-lipoprotein-lipase
#1
Xun Chi, Emily C Britt, Hannah W Shows, Alexander J Hjelmaas, Shwetha K Shetty, Emily M Cushing, Wendy Li, Alex Dou, Ren Zhang, Brandon S J Davies
OBJECTIVE: Several members of the angiopoietin-like (ANGPTL) family of proteins, including ANGPTL3 and ANGPTL8, regulate lipoprotein lipase (LPL) activity. Deficiency in either ANGPTL3 or ANGPTL8 reduces plasma triglyceride levels and increases LPL activity, whereas overexpression of either protein does the opposite. Recent studies suggest that ANGPTL8 may functionally interact with ANGPTL3 to alter clearance of plasma triglycerides; however, the nature of this interaction has remained elusive...
October 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/29021760/saponins-and-flavonoids-from-adzuki-bean-vigna-angularis-l-ameliorate-high-fat-diet-induced-obesity-in-icr-mice
#2
Rui Liu, Yinan Zheng, Zongwei Cai, Baojun Xu
Background and purpose: As an herbal medicine, adzuki bean has been practiced since the Tang Dynasty of China to maintain health and control weight; this practice is still very popular in China nowadays. However, it is still lack of sufficient scientific basis to explain scientific principle of this popular civil practice in weight control using adzuki bean. The purpose of this study was to verify and explain the anti-obesity effects of adzuki bean through in vitro enzymatic assays, in vitro lipolysis and in vivo study of obese mice model...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28970555/impact-of-endothelial-lipase-on-cholesterol-efflux-capacity-of-serum-and-high-density-lipoprotein
#3
Irene Schilcher, Sabine Kern, Andelko Hrzenjak, Thomas O Eichmann, Tatjana Stojakovic, Hubert Scharnagl, Madalina Duta-Mare, Dagmar Kratky, Gunther Marsche, Saša Frank
Endothelial lipase (EL) is a potent modulator of the structural and functional properties of HDL. Impact of EL on cholesterol efflux capacity (CEC) of serum and isolated HDL is not well understood and apparently contradictory data were published. Here, we systematically examined the impact of EL on composition and CEC of serum and isolated HDL, in vitro and in vivo, using EL-overexpressing cells and EL-overexpressing mice. CEC was examined in a validated assay using (3)H-cholesterol labelled J774 macrophages...
October 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28966096/evaluation-of-the-digestibility-of-solid-lipid-nanoparticles-of-glyceryl-dibehenate-produced-by-two-techniques-ultrasonication-and-spray-flash-evaporation
#4
Vincent Jannin, Lucia Blas, Stéphanie Chevrier, Cédric Miolane, Frédéric Demarne, Denis Spitzer
OBJECTIVE: To evaluate the digestibility of Solid Lipid Nanoparticles (SLN) of glyceryl dibehenate prepared either with surfactants by ultrasonication or without surfactant by spray-flash evaporation. METHODS: SLN of glyceryl dibehenate (Compritol® 888 ATO) were produced by two processes: (i) high-shear homogenization with a solution of water-soluble surfactants followed by ultrasonication (ii) and Spray-Flash Evaporation (SFE) of the pure lipid. The digestibility of these nanoparticles was then tested by in vitro lipolysis using a pH-stat apparatus and the assay of glycerides by gel phase chromatography...
September 29, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28963949/dacryodes-edulis-enhances-antioxidant-activities-suppresses-dna-fragmentation-in-oxidative-pancreatic-and-hepatic-injuries-and-inhibits-carbohydrate-digestive-enzymes-linked-to-type-2-diabetes
#5
Ochuko L Erukainure, Ramgopal Mopuri, Olajumoke A Oyebode, Neil A Koorbanally, Md Shahidul Islam
The leaves of Dacryodes edulis were investigated for their anti-oxidative and anti-diabetic potentials in vitro. Extracts from sequential extraction with solvents of increasing polarity (n-hexane, ethyl acetate, ethanol and aqueous) of the leaves were subjected to in vitro antioxidant assays using the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) scavenging and Ferric reducing antioxidant power (FRAP) protocols respectively. Their inhibitory effects were investigated on α-glucosidase, pancreatic lipases, pancreatic ATPase and glucose-6-phospatase activities...
September 27, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28963856/dopamine-d2-receptor-signaling-controls-inflammation-in-acute-pancreatitis-via-pp2a-dependent-akt-nf%C3%AE%C2%BAb-signaling-pathway
#6
Xiao Han, Bin Li, Xin Ye, Tunike Mulatibieke, Jianghong Wu, Juanjuan Dai, Deqing Wu, Jianbo Ni, Ruling Zhang, Jing Xue, Rong Wan, Xingpeng Wang, Guoyong Hu
BACKGROUND AND PURPOSE: Dopamine (DA) has multiple anti-inflammatory effects, but its role and molecular mechanism in acute pancreatitis (AP) is unclear. We investigated the role of DA signaling on inflammatory response in AP. EXPERIMENTAL APPROACH: We analyzed the changes of pancreatic dopaminergic system and effects of DA, antagonists and agonists of DA receptor (DRD)1 and 2 in wild-type and pancreas-specific Drd2(-/-) mice with AP (induced by caerulein and lipopolysaccharide or L-Arginine) or in pancreatic acinar cells with or without CCK stimulation...
September 30, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28958971/study-of-two-comparison-procedures-applied-to-biochemical-results-from-twin-analyzers
#7
Lise Larcher, Laurence Pacot, Sophie Bailleul, Guillaume Lefèvre
Two procedures for checking agreement between twin analyzers (Abbott Architect ci 8200) were tested in 23 blood and 7 urine parameters (10,160 paired results from 7,882 blood and 2,278 urine tests). Two protocols were compared. In protocol 1, acceptance criterion is based on standard-deviation originated either from French recommendations (Société française de biologie clinique) or from within subject biological variation. In protocol 2, acceptance criterion is based on values of expanded uncertainty of measurements calculated according to SH GTA 04...
October 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/28946872/suppression-of-adipocyte-differentiation-and-lipid-accumulation-by-stearidonic-acid-sda-in-3t3-l1-cells
#8
Yueru Li, Yinghui Rong, Lisui Bao, Ben Nie, Guang Ren, Chen Zheng, Rajesh Amin, Robert D Arnold, Ramesh B Jeganathan, Kevin W Huggins
BACKGROUND: Increased consumption of omega-3 (ω-3) fatty acids found in cold-water fish and fish oil has been reported to protect against obesity. A potential mechanism may be through reduction in adipocyte differentiation. Stearidonic acid (SDA), a plant-based ω-3 fatty acid, has been targeted as a potential surrogate for fish-based fatty acids; however, its role in adipocyte differentiation is unknown. This study was designed to evaluate the effects of SDA on adipocyte differentiation in 3T3-L1 cells...
September 25, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28941907/genotyping-identification-and-multifunctional-features-of-yeasts-associated-to-bosana-naturally-black-table-olive-fermentations
#9
Cinzia Porru, Francisco Rodríguez-Gómez, Antonio Benítez-Cabello, Rufino Jiménez-Díaz, Giacomo Zara, Marilena Budroni, Ilaria Mannazzu, Francisco Noé Arroyo-López
Directly brined black table olives of Bosana variety are a traditional food product of Sardinia island (Italy), spontaneously fermented by yeasts among other microorganisms. However, as far as we know, the identification, biotechnological and probiotic potential of this yeast community has not been investigated yet. In this work, a total of 72 yeast isolates previously obtained from Bosana olive brines were first genotyped by Random Amplified Polymorphic DNA (RAPD-PCR) analysis with primer M13, and then identified by sequencing of D1/D2 domains of rDNA 26S gene...
February 2018: Food Microbiology
https://www.readbyqxmd.com/read/28923075/lipase-and-biosurfactant-from-ochrobactrum-intermedium-strain-mzv101-isolated-by-washing-powder-for-detergent-application
#10
Mina Zarinviarsagh, Gholamhossein Ebrahimipour, Hossein Sadeghi
BACKGROUND: Alkaline thermostable lipase and biosurfactant producing bacteria are very interested at detergent applications, not only because of their eco-friendly characterize, but alsoproduction lipase and biosurfactant by using cheap materials. Ochrobactrum intermedium strain MZV101 was isolated as washing powder resistant, alkaline thermostable lipase and biosurfactant producing bacterium in order to use at detergent applications. METHODS: O. intermedium strain MZV101 produces was lipase and biosurfactant in the same media with pH 10 and temperature of 60 °C...
September 18, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28894700/targeting-the-type-ii-secretion-system-development-optimization-and-validation-of-a-high-throughput-screen-for-the-identification-of-small-molecule-inhibitors
#11
Ursula Waack, Tanya L Johnson, Khalil Chedid, Chuanwu Xi, Lyle A Simmons, Harry L T Mobley, Maria Sandkvist
Nosocomial pathogens that develop multidrug resistance present an increasing problem for healthcare facilities. Due to its rapid rise in antibiotic resistance, Acinetobacter baumannii is one of the most concerning gram-negative species. A. baumannii typically infects immune compromised individuals resulting in a variety of outcomes, including pneumonia and bacteremia. Using a murine model for bacteremia, we have previously shown that the type II secretion system (T2SS) contributes to in vivo fitness of A. baumannii...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28884505/alternative-oils-tested-as-feedstocks-for-enzymatic-fames-synthesis-toward-a-more-sustainable-process
#12
Belén Infanzón, Silvia Cesarini, Josefina Martínez, F I Javier Pastor, Pilar Diaz
Previously isolated and characterized Pseudomonas lipases were immobilized in a low-cost MP-1000 support by a re-loading procedure that allowed a high activity per weight of support. Immobilized LipA, LipC, and LipCmut lipases, and commercial Novozym® 435 were tested for fatty acid methyl ester (FAMEs) synthesis using conventional and alternative feedstocks. Triolein and degummed soybean oils were used as model substrates, whereas waste cooking oil and M. circinelloides oil were assayed as alternative, low cost feedstocks, whose free fatty acid (FFA), and acylglyceride profile was characterized...
September 7, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28881270/molecular-and-clinical-characterization-of-a-series-of-patients-with-childhood-onset-lysosomal-acid-lipase-deficiency-retrospective-investigations-follow-up-and-detection-of-two-novel-lipa-pathogenic-variants
#13
Livia Pisciotta, Giulia Tozzi, Lorena Travaglini, Roberta Taurisano, Tiziano Lucchi, Giuseppe Indolfi, Francesco Papadia, Maja Di Rocco, Lorenzo D'Antiga, Patricia Crock, Komal Vora, Scott Nightingale, Helen Michelakakis, Anastasia Garoufi, Lilia Lykopoulou, Stefano Bertolini, Sebastiano Calandra
BACKGROUND AND AIMS: Childhood/Adult-onset Lysosomal Acid Lipase Deficiency (LAL-D) is a recessive disorder due to loss of function variants of LAL, the enzyme which hydrolyses cholesteryl esters, derived from internalized apoB containing lipoproteins. The disease is characterized by multi-organ involvement including the liver, spleen, intestine and cardiovascular system. The aim of this study was the clinical and molecular characterization of 14 (13 unrelated) previously unreported patients with childhood-onset LAL-D...
October 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28857479/crystal-structure-of-a-lipase-from-streptomyces-sp-strain-w007-implications-for-thermostability-and-regiospecificity
#14
Zexin Zhao, Shulin Hou, Dongming Lan, Xiumei Wang, Jinsong Liu, Faez Iqbal Khan, Yonghua Wang
MAS1 from marine Streptomyces sp. strain W007 belongs to the bacterial lipase I.7 subfamily and is characterized as a thermostable and nonregiospecific lipase. To shed light on the catalytic mechanism of MAS1, we determined its crystal structure with closed conformation in two crystal forms at 2.3 Å resolution. MAS1 adopts the canonical α/β hydrolase core fold with its catalytic triad being formed by S109, D200 and H232. Structural analysis and biochemical assays revealed that disulfide bonds and salt bridges play a vital role in the thermostability of MAS1...
August 31, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28855441/microrna-182-promotes-lipoprotein-lipase-expression-and-atherogenesisby-targeting-histone-deacetylase-9-in-apolipoprotein-e-knockout-mice
#15
Hai-Peng Cheng, Duo Gong, Zhen-Wang Zhao, Ping-Ping He, Xiao-Hua Yu, Qiong Ye, Chong Huang, Xin Zhang, Ling-Yan Chen, Wei Xie, Min Zhang, Liang Li, Xiao-Dan Xia, Xin-Ping Ouyang, Yu-Lin Tan, Zong-Bao Wang, Guo-Ping Tian, Xi-Long Zheng, Wei-Dong Yin, Chao-Ke Tang
BACKGROUND: Lipoprotein lipase (LPL) expressed in macrophages plays an important role in promoting the development of atherosclerosis or atherogenesis. MicroRNA-182 (miR-182) is involved in the regulation of lipid metabolism and inflammation. However, it remains unclear how miR-182 regulates LPL and atherogenesis.Methods and Results:Using bioinformatics analyses and a dual-luciferase reporter assay, we identified histone deacetylase 9 (HDAC9) as a target gene of miR-182. Moreover, miR-182 upregulated LPL expression by directly targetingHDAC9in THP-1 macrophages...
August 29, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/28844762/2-arachidonoylglycerol-metabolism-is-differently-modulated-by-oligomeric-and-fibrillar-conformations-of-amyloid-beta-in-synaptic-terminals
#16
Ana C Pascual, Virginia L Gaveglio, Norma M Giusto, Susana J Pasquaré
Alzheimer's disease (AD) is the most prevalent disorder of senile dementia mainly characterized by amyloid-beta peptide (Aβ) deposits in the brain. Cannabinoids are relevant to AD as they exert several beneficial effects in many models of this disease. Still, whether the endocannabinoid system is either up- or down-regulated in AD has not yet been fully elucidated. Thus, the aim of the present paper was to analyze endocannabinoid 2-arachidonoylglycerol (2-AG) metabolism in cerebral cortex synaptosomes incubated with Aβ oligomers or fibrils...
August 24, 2017: Neuroscience
https://www.readbyqxmd.com/read/28808476/bark-extracts-of-ceylon-cinnamon-possess-antilipidemic-activities-and-bind-bile-acids-in-vitro
#17
Walimuni Prabhashini Kaushalya Mendis Abeysekera, Sirimal Premakumara Galbada Arachchige, Wanigasekera Daya Ratnasooriya
Ethanol (95%) and dichloromethane : methanol (1 : 1) bark extracts of authenticated Ceylon cinnamon were investigated for range of antilipidemic activities (ALA): HMG-CoA reductase, lipase, cholesterol esterase, and cholesterol micellization inhibitory activities and bile acids binding in vitro. Individual compounds in bark extracts were also evaluated. Bark extracts showed ALA in all the assays studied. The IC50 (μg/mL) values ranged within 153.07 ± 8.38-277.13 ± 32.18, 297.57 ± 11.78-301.09 ± 4...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28797651/biological-effects-and-chemical-characterization-of-iris-schachtii-markgr-extracts-a-new-source-of-bioactive-constituents
#18
Andrei Mocan, Gokhan Zengin, Adriano Mollica, Ahmet Uysal, Erdogan Gunes, Gianina Crișan, Abdurrahman Aktumsek
This study gathers information about the effects of different extracts (methanol and water) from rhizomes and aerial parts of Iris schachtii on selected enzymes (cholinesterases, alpha-amylase and alpha-glucosidase, tyrosinase and lipase) as well as on their antioxidant capacities and antimutagenic properties in relation with their phenolic composition. The chemical composition was assessed by determining total phenolic and flavonoid content as well as individual phenolic compounds by HPLC-DAD. Moreover, antioxidant abilities of the investigated extracts were tested by using different assays including free radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating, overall rhizomes being indicated as a superior source of antioxidant compounds...
August 7, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28791824/anti-enterococcal-activities-of-pentacyclic-triterpenes
#19
Dorota Wojnicz, Dorota Tichaczek-Goska, Kamila Korzekwa, Marta Kicia, Andrzej Hendrich
BACKGROUND: Asiatic (AA) and ursolic (UA) acids are widely studied phytochemicals, but their antimicrobial properties are still poorly understood. Therefore our research has focused on their activity against uropathogenic Enterococcus faecalis strains. OBJECTIVES: The aim of this research was to determine the influence of AA and UA on the growth, cell morphology, virulence factors and biofilm formation by E. faecalis strains. MATERIAL AND METHODS: AA and UA were purchased from Sigma-Aldrich...
May 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/28780114/development-of-a-novel-loop-mediated-isothermal-amplification-assay-for-the-detection-of-lipolytic-pseudomonas-fluorescens-in-raw-cow-milk-from-north-china
#20
Liang Xin, Lanwei Zhang, Zhaoxu Meng, Kai Lin, Shuang Zhang, Xue Han, HuaXi Yi, Yanhua Cui
Lipases secreted by psychrotrophic bacteria are known to be heat resistant and can remain active even after the thermal processing of milk products. Such enzymes are able to destabilize the quality of milk products by causing a rancid flavor. Rapid detection of a small amount of heat-resistant lipase-producing psychrotrophic bacteria is crucial for reducing their adverse effects on milk quality. In this study, we established and optimized a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Pseudomonas fluorescens in raw cow milk, as the most frequently reported heat-resistant lipase-producing bacterial species...
August 2, 2017: Journal of Dairy Science
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