keyword
MENU ▼
Read by QxMD icon Read
search

ep4

keyword
https://www.readbyqxmd.com/read/29217623/prostaglandins-d2-and-e2-have-opposite-effects-on-alveolar-macrophage-infected-with-histoplasma-capsulatum
#1
Priscilla A T Pereira, Patrícia A Assis, Morgana K B Prado, Simone Gusamão Ramos, David M Aronoff, Francisco W G Paula-Silva, Carlos A Sorgi, Lúcia H Faccioli
PGE2 suppresses macrophage effector mechanisms; however, little is known about the function of PGD2 in infected alveolar macrophages (AMs). Using serum opsonized Histoplasma capsulatum (ops-H. capsulatum) in vitro, we demonstrated that AMs produced PGE2 and PGD2 in a time-dependent manner, with PGE2 levels exceeding those of PGD2 by 48 h post-infection. Comparison of the effects of both exogenous PGs on AMs revealed that PGD2 increased phagocytosis and killing through the DP2 receptor, whereas PGE2 had opposite effects, through EP2/EP4-dependent mechanisms...
December 7, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/29217133/pge2-suppresses-human-group-2-innate-lymphoid-cell-function
#2
Jovana Maric, Avinash Ravindran, Luca Mazzurana, Åsa K Björklund, Aline Van Acker, Anna Rao, Danielle Friberg, Sven-Erik Dahlén, Akos Heinemann, Viktoria Konya, Jenny Mjösberg
BACKGROUND: Group 2 innate lymphoid cells (ILC2) are involved in the initial phase of type 2 inflammation and can amplify allergic immune responses by orchestrating other type 2 immune cells. PGE2 is a bioactive lipid that plays protective roles in the lung, particularly during allergic inflammation. OBJECTIVE: We set out to investigate how PGE2 regulates human ILC2 function. METHODS: The effects of PGE2 on human ILC2 proliferation, intracellular cytokine and transcription factor expression were assessed by flow cytometry...
December 4, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29203142/synthesis-and-evaluation-of-a-potent-well-balanced-ep2-ep3-dual-agonist
#3
Akihiro Kinoshita, Masato Higashino, Koji Yoshida, Yoshiyuki Aratani, Akito Kakuuchi, Keisuke Hanada, Hiroyuki Takeda, Atsushi Naganawa, Hidekazu Matsuya, Kazuyuki Ohmoto
A highly potent and well-balanced dual agonist for the EP2 and EP3 receptors is described. Optimization of the lead compound was accomplished in consideration of the relative agonist activity against each EP subtype receptor and the pharmacokinetic profile. As the result, 2-[(2-{(1R,2R)-2-[(1E,4S)-5-cyclopentyl-4-hydroxy-4-methyl-1-penten-1-yl]-5-oxocyclopentyl}eth-yl)thio]-1,3-thiazole-4-carboxylic acid (10) showed excellent potency (human EC50 EP2 = 1.1 nM, EP3 = 1.0 nM) with acceptable selectivity over the EP1 and EP4 subtypes (>2000-fold)...
November 24, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29194752/the-regulatory-role-of-the-oral-commensal-streptococcus-mitis-on-human-monocytes
#4
Stian André Engen, Olav Schreurs, Fernanda Petersen, Inger Johanne Schytte Blix, Espen S Baekkevold, Karl Schenck
Streptococcus mitis colonizes all niches of the human oral cavity from early infancy and throughout life. Monocytes patrol blood vessels, lymphoid and non-lymphoid tissues, and migrate into infected tissue where they participate in the inflammatory cascade and immune regulation. Here, we studied the effect of S. mitis on monocytes. Transcriptome analysis of monocytes exposed to S. mitis (SmMo) revealed increased transcription of chemotactic factors (CCL2, CCL3, CCL20, CXCL1, CXCL2) and cytokines (IL1A, IL1B, IL6, IL23, IL36G, TNF), indicating that S...
December 1, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/29193208/granular-cell-basal-cell-carcinoma-a-case-report
#5
Xinyi Ma, Guihua Wang, Aditya Kuwadekar, Min Zhao, Weili Hu, Yeqin Wu, Zhongliang Yi, Xianen Xu, Li Xu
Granular cell basal cell carcinoma (BCC) is a rare histopathological variant of BCC, Our review of the literature revealed only 17 previously identified cases. We report the case of a 47-year-old man who presented with an ulceration on his right upper lip which was subsequently removed. Histopathologic examination revealed that the tumor was composed solely of granular cells with numerous cytoplasmic eosinophilic round inclusion bodies. Mitotic figures ranged from 8 to 15 per 10 high-power fields with a Ki-67 proliferative index approximated at 5%...
November 28, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/29190893/effect-of-pge2-eps-pathway-on-primary-cultured-rat-neuron-injury-caused-by-aluminum
#6
Lu Yang, Yuling Wei, Ying Luo, Qunfang Yang, Huan Li, Congli Hu, Yang Yang, Junqing Yang
To observe the characteristic changes of PGE2-EPs pathway and divergent functions of PGE2 receptor subtypes on neuronal injury. The primary cultured rat hippocampus neuron injury model was established via aluminum maltolate (100 μM). The aluminum-overload neurons were treated with the agonists of EP1 (17-phenyl trinor Prostaglandin E2 ethyl amide), EP2 (Butaprost), EP3 (Sulprostone) and EP4 (CAY10598) and antagonists of EP1 (SC-19220), EP2 (AH6809) and EP4 (L-161982) at different concentrations, respectively...
November 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29163789/prostaglandin-e2-and-pd-1-mediated-inhibition-of-antitumor-ctl-responses-in-the-human-tumor-microenvironment
#7
Jie Miao, Xu Lu, Yuefeng Hu, Chunmei Piao, Xuan Wu, Xuesong Liu, Caiting Huang, Yue Wang, Dan Li, Jingwei Liu
Accumulating evidence indicates that inflammation plays a critical role in cancer development; however, mechanisms of immunosuppression hinder productive anti-tumor immunity to limit immunopathology. Tumor-specific cytotoxic T lymphocyte (CTL) dysfunction or exhaustion by upregulating inhibitory receptors such as programmed cell death 1 (PD-1) in tumor-bearing hosts is one such mechanism. Identification and blockade of the pathways that induce CTL dysfunction has been shown to partially restore CTL function in tumor-bearing hosts...
October 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/29146573/prostanoid-ep4-agonist-l-902-688-activates-ppar%C3%AE-and-attenuates-pulmonary-arterial-hypertension
#8
Hsin-Hsien Li, Hsao-Hsun Hsu, Gwo-Jyh Chang, I-Chen Chen, Wan-Jing Ho, Pei-Chen Hsu, Wei-Jan Chen, Jong-Hwei Su Pang, Chung-Chi Huang, Ying-Ju Lai
Prostacyclin agonists that bind the prostacyclin receptor (IP) to stimulate cyclic adenosine monophosphate (cAMP) synthesis are effective vasodilators for the treatment of idiopathic pulmonary arterial hypertension (IPAH) but this signaling may occur through nuclear peroxisome proliferator-activated receptor-gamma (PPARγ). There is evidence of scant IP and PPARγ expression but stable prostanoid EP4 receptor (EP4) expression in IPAH patients. Both IP and EP4 functionally couple with stimulatory G protein (Gs), which activate signal transduction...
November 16, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29145862/l161982-alleviates-collagen-induced-arthritis-in-mice-by-increasing-treg-cells-and-down-regulating-interleukin-17-and-monocyte-chemoattractant-protein-1-levels
#9
Liang Chen, Xianglei Wu, Jun Zhong, Dongqing Li
BACKGROUND: To investigate the effects and potential mechanism of L161982 (a kind of EP4 antagonist) on the collagen-induced arthritis (CIA) mice model. METHODS: The CIA mice model were first established by immunizing with Chicken Type II Collagen on DBA/1 mice. The CIA groups were administered once a day for 2 weeks with either 5 mg/kg L161982 by intraperitoneal injections (IP), 200 U celecoxib by intragastrical injections, or 100 μl PBS (IP). At the end of the study, total arthritis score and histopathologic examination were assessed to determine CIA severity...
November 16, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29133212/quantitative-and-systems-pharmacology-2-in-silico-polypharmacology-of-g-protein-coupled-receptor-ligands-via-network-based-approaches
#10
Zengrui Wu, Weiqiang Lu, Weiwei Yu, Tianduanyi Wang, Weihua Li, Guixia Liu, Hankun Zhang, Xiufeng Pang, Jin Huang, Mingyao Liu, Feixiong Cheng, Yun Tang
G protein-coupled receptors (GPCRs) are the largest super family with more than 800 membrane receptors. Currently, over 30% of the approved drugs target human GPCRs. However, only approximately 30 human GPCRs have been resolved three-dimensional crystal structures, which limits traditional structure-based drug discovery. Recent advances in network-based systems pharmacology approaches have demonstrated powerful strategies for identifying new targets of GPCR ligands. In this study, we proposed a network-based systems pharmacology framework for comprehensive identification of new drug-target interactions on GPCRs...
November 10, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29129801/activating-prostaglandin-e2-receptor-subtype-ep4-increases-secreted-mucin-from-airway-goblet-cells
#11
Tomohiro Akaba, Kosaku Komiya, Isao Suzaki, Yuji Kozaki, Jun Tamaoki, Bruce K Rubin
Prostaglandin E2 (PGE2) is a ligand of the E-type prostanoid receptors, EP1-4. PGE2 secretion is increased in the airways of patients with asthma by secretory phospholipases A2, which also increases MUC5AC mucin in goblet cells. We hypothesized that PGE2 would also increase MUC5AC mRNA and secreted protein through specific EP receptor activation. We sought to assess the effect of specific EP receptor activation on MUC5AC secretion from ciliated-enriched cells or goblet-enriched cells induced by IL-13. We develop an enriched goblet cell epithelium by growing normal human bronchial epithelial cells at air liquid interface for 14 days in the presence of IL-13...
November 9, 2017: Pulmonary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/29123257/prostaglandin-receptors-induce-urothelial-tumourigenesis-as-well-as-bladder-cancer-progression-and-cisplatin-resistance-presumably-via-modulating-pten-expression
#12
Eiji Kashiwagi, Satoshi Inoue, Taichi Mizushima, Jinbo Chen, Hiroki Ide, Takashi Kawahara, Leonardo O Reis, Alexander S Baras, George J Netto, Hiroshi Miyamoto
BACKGROUND: We investigated the role of prostaglandin receptors (e.g. prostaglandin E2 receptor 2 (EP2), EP4) and the efficacy of celecoxib in urothelial tumourigenesis and cancer progression. METHODS: We performed immunohistochemistry in bladder cancer (BC) tissue microarrays, in vitro transformation assay in a normal urothelial SVHUC line, and western blot/reverse transcription-polymerase chain reaction/cell growth assays in BC lines. RESULTS: EP2/EP4 expression was elevated in BCs compared with non-neoplastic urothelial tissues and in BCs from those who were resistant to cisplatin-based neoadjuvant chemotherapy...
November 9, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/29100274/nicotine-induces-ep4-receptor-expression-in-lung-carcinoma-cells-by-acting-on-ap-2%C3%AE-the-intersection-between-cholinergic-and-prostanoid-signaling
#13
Yu Fan, Ke Wang
It was demonstrated that nicotine increased non-small cell lung cancer cell proliferation through nicotinic acetylcholine receptor -mediated signals. However, the detailed mechanism remains incompletely understood. We evaluated whether nicotine increased EP4 receptor expression in lung carcinoma cells by activating on AP-2α. METHODS: The non-small cell lung cancer cells of A549 and H1838 were cultured and treated with EP4 inhibitor AH23848, also with EP4 and control siRNAs. The extracellular signal-regulated kinases inhibitor PD98059, the p38 mitogen-activated protein kinase inhibitor SB239063, the α7 nicotinic acetylcholine receptor inhibitor α-bungarotoxin, the α4 nicotinic acetylcholine receptor inhibitor dihydro-β-erythroidine, the PI3K inhibitor wortmannin, the PKC inhibitor calphostin C, and the PKA inhibitor H89 have been used to evaluate the effects on proliferations...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29073599/combination-of-solamargine-and-metformin-strengthens-igfbp1-gene-expression-through-inactivation-of-stat3-and-reciprocal-interaction-between-foxo3a-and-sp1
#14
Qing Tang, Fang Zheng, JingJing Wu, Qian Xiao, Liuning Li, Swei Sunny Hann
BACKGROUND/AIMS: Solamargine, one natural photochemical component from traditional plants, has been shown to have anti-cancers properties. We previously showed that solamargine inhibited the growth of non-small-cell lung cancer (NSCLC) cells through suppression of prostaglandin E2 (PGE2) receptor EP4 gene and regulation of downstream signaling pathways. However, the detailed mechanism underlying this, especially in combination of metformin, a known AMPK activator, still remained to be determined...
October 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29073216/prolonged-immune-alteration-following-resolution-of-acute-inflammation-in-humans
#15
Madhur P Motwani, Justine Newson, Simon Kwong, Angela Richard-Loendt, Romain Colas, Jesmond Dalli, Derek W Gilroy
Acute inflammation is an immediate response to infection and injury characterised by the influx of granulocytes followed by phagocytosing mononuclear phagocytes. Provided the antigen is cleared and the immune system of the host is fully functional, the acute inflammatory response will resolve. Until now it is considered that resolution then leads back to homeostasis, the physiological state tissues experienced before inflammation occurred. Using a human model of acute inflammation driven by intradermal UV killed Escherichia coli, we found that bacteria and granulocyte clearance as well as pro-inflammatory cytokine catabolism occurred by 72h...
2017: PloS One
https://www.readbyqxmd.com/read/29053493/induction-of-desmoplastic-trichoepithelioma-in-a-dermatofibroma
#16
Mihaela Georgescu, Norbert Blödorn-Schlicht, Almut Böer-Auer
Induction of follicular germinative structures above a dermatofibroma (DF) is a common finding. Rarely, induction of a trichoblastoma in a DF has been observed. Here, we report the case of a desmoplastic trichoepithelioma induced by a DF. The lesion with clinical and histological appearance of a DF situated on the left dorsal foot showed an associated adnexal proliferation that fulfilled histopathological criteria of desmoplastic trichoepithelioma. Immunohistochemistry (Ber-EP4, Bcl-2, CK17, CK20, CK7, EMA, and Ki67) helped to confirm the diagnosis and to exclude possible differential diagnoses...
October 2, 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/29048219/immunohistochemistry-in-peritoneal-mesothelioma-a-single-center-experience-of-244-cases
#17
Radhika Tandon, Yuis Jimenez-Cortez, Robert Taub, Alain C Borczuk
CONTEXT: - Diagnosis of malignant mesothelioma is more common in the chest than it is in the abdomen. Most published immunohistochemistry data are more applicable to pleural than to peritoneal mesothelioma. OBJECTIVE: - To clarify the practical utility of 17 immunohistochemistry markers in the differential diagnosis of peritoneal mesothelioma with an emphasis on stains for which there is either contradictory information or a paucity of literature. DESIGN: - Consultation files of peritoneal mesothelioma diagnoses rendered from 1999 to 2014 were reviewed; 242 cases are identified...
October 19, 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/29024544/squamous-cell-carcinoma-in-serous-effusions-avoiding-pitfalls-in-this-rare-encounter
#18
Christopher LePhong, Elizabeth W Hubbard, Stuart Van Meter, Laurentia Nodit
BACKGROUND: With advent of personalized medicine, precise classification of malignant tumors becomes essential. Squamous cell carcinoma (SCC) is rarely found in serous effusions and has morphologic and immunohistochemical (IHC) overlap with other neoplasms. METHODS: 17-year review identified 49 fluids from 26 patients where SCC was recognized. RESULTS: SCC was more frequent in pleural fluid (84%) and rare in other effusions. Lung SCC was common (65%), followed by head and neck (16%), with other origins less represented...
October 11, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28978522/cigarette-smoke-activates-cftr-through-ros-stimulated-camp-signaling-in-human-bronchial-epithelial-cells
#19
Francis H Wong, Asmahan AbuArish, Elizabeth Matthes, Mark J Turner, Lana E Greene, Alexandre Cloutier, Renaud Robert, David Y Thomas, Gonzalo Cosa, Andre M Cantin, John W Hanrahan
Air pollution stimulates airway epithelial secretion through a cholinergic reflex that is unaffected in cystic fibrosis (CF), yet a strong correlation is observed between passive smoke exposure in the home and impaired lung function in CF children. Our aim was to study the effects of low smoke concentrations on CFTR function in vitro. Cigarette smoke extract stimulated robust anion secretion that was transient, mediated by cystic fibrosis transmembrane conductance regulator (CFTR), and dependent on cAMP-dependent protein kinase activation...
October 4, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28963736/expression-of-gnrh-receptor-in-the-canine-corpus-luteum-and-luteal-function-following-deslorelin-acetate-induced-puberty-delay
#20
D Kaya, A Gram, M P Kowalewski, S Schäfer-Somi, M Kuru, A Boos, S Aslan
The goals of this study were as follows: (Experiment 1) to examine the basic capability of canine corpora lutea (CL) to respond to GnRH by assessing expression of gonadotropin-releasing hormone receptor (GnRH-R) in luteal samples collected throughout the luteal lifespan from non-pregnant dogs, and (Experiment 2) to investigate the effects of pre-pubertal application of the GnRH agonist deslorelin acetate on luteal function following the first oestrus. Mature CL were collected during the mid-luteal phase (days 30-45) from treated and control bitches...
September 30, 2017: Reproduction in Domestic Animals, Zuchthygiene
keyword
keyword
114898
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"