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https://www.readbyqxmd.com/read/29127055/fractionation-separation-of-human-plasma-proteins-using-hplc-with-a-homemade-iron-porphyrin-based-monolithic-column
#1
Doudou Zhang, Yu Zhao, Dandan Lan, Xiaomin Pang, Ligai Bai, Haiyan Liu, Hongyuan Yan
In this work a polymer monolithic column was fabricated within the confines of a stainless steel column (50×4.6mm i.d.) via radical polymerization by using iron porphyrin and butyl methacrylate as co-monomers, ethylene glycol dimethacrylate as crosslinking agent, ethylene glycol, isopropyl alcohol and N, N-dimethylformamide as tri-porogens, benzoyl peroxide and N,N-dimethylaniline as initiators. The resulting monolithic column was characterized by elemental analysis, scanning electron microscopy, nitrogen adsorption BET surface area, and mercury intrusion porosimetry, respectively...
November 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29114901/allergic-contact-dermatitis-caused-by-calcipotriol
#2
Liesbeth Gilissen, Sara Huygens, An Goossens
BACKGROUND: Calcipotriol, a synthetic vitamin D analogue, is widely used for the topical treatment of psoriasis. It often causes irritant reactions, whereas allergic contact dermatitis has less commonly been reported. OBJECTIVES: To report on 6 patients (among them an 11-year-old child) who presented with eczematous lesions complicating pre-existing dermatitis, observed in our tertiary referral patch test clinic between 2004 and 2016. METHODS: Patch tests were performed with the commercial preparation used by the patients and/or its ingredients, including calcipotriol (2 or 10 µg/ml in isopropyl alcohol), according to ESCD patch test guidelines...
November 8, 2017: Contact Dermatitis
https://www.readbyqxmd.com/read/29109746/comparative-evaluation-of-root-canal-morphology-of-mandibular-premolars-using-clearing-and-cone-beam-computed-tomography
#3
Abbasali Khademi, Mojdeh Mehdizadeh, Maryam Sanei, Hamidreza Sadeqnejad, Saber Khazaei
Background: Different techniques are used to evaluate the anatomy of root canal system. The present study was aimed to evaluate the root canal morphology of mandibular premolars using clearing and cone beam computed tomography (CBCT) techniques. Materials and Methods: A total of 182 mandibular first and second premolars were evaluated in vitro using 100 μm CBCT cross sections. The root canal morphology of the teeth was determined based on Vertucci classification in relation to the prevalence of C-shaped canals, lateral canals, and furcation location...
September 2017: Dental Research Journal
https://www.readbyqxmd.com/read/29096550/optimization-of-nutraceutical-coenzyme-q10-nanoemulsion-with-improved-skin-permeability-and-anti-wrinkle-efficiency
#4
Eman S El-Leithy, Amna M Makky, Abeer M Khattab, Doaa G Hussein
Coenzyme Q10 (CoQ10) is an insoluble, poorly permeable antioxidant with great biological value which acts as anti-aging and anti-wrinkle agent. To improve its permeability through topical application, the current study aimed at formulating oil/water (o/w) nanoemulsion (NE) as an efficient vehicle for delivering (CoQ10) through the skin barriers. The solubility of (CoQ10) was tested for various oils, surfactants (S), and co-surfactants (CoS). The NE region was determined by constructing pseudoternary phase diagrams...
November 2, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/29074717/2-chlorhexidine-70-isopropyl-alcohol-versus-10-povidone-iodine-for-insertion-site-cleaning-before-central-line-insertion-in-preterm-infants-a-randomised-trial
#5
Emily A Kieran, Anne O'Sullivan, Jan Miletin, Anne R Twomey, Susan J Knowles, Colm Patrick Finbarr O'Donnell
OBJECTIVE: To determine whether 2% chlorhexidine gluconate-70% isopropyl alcohol (CHX-IA) is superior to 10% aqueous povidone-iodine (PI) in preventing catheter-related blood stream infection (CR-BSI) when used to clean insertion sites before placing central venous catheters (CVCs) in preterm infants. DESIGN: Randomised controlled trial. SETTING: Two neonatal intensive care units (NICUs). PATIENTS: Infants <31 weeks' gestation who had a CVC inserted...
October 26, 2017: Archives of Disease in Childhood. Fetal and Neonatal Edition
https://www.readbyqxmd.com/read/29071228/assay-of-desmopressin-acetate-in-nasal-spray-development-of-validated-pre-column-hplc-fluorescence-method
#6
Neeraj Upmanyu, Pawan Kumar Porwal
Purpose: Desmopressin acetate (DDAPV), a synthetic analogue of vasopressin, has been recommended to be used in diabetes insipidus, mild forms of hemophilia and Von Willebrand disease. The DDAPV is available for adminstration via different routes viz. oral, parenteral and nasal, however its dose is very less in case of nasal sprays (20 µg) and parenteral route (4 µg) compared to oral route (0.1 to 0.3 mg in tablet). A sensitive and selective method is needed to be developed and validated for assay of low concentrations of DDAPV in its pharmaceutical dosage form i...
September 2017: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28966560/crystal-nucleation-using-surface-energy-modified-glass-substrates
#7
Kyle A Nordquist, Kevin M Schaab, Jierui Sha, Andrew H Bond
Systematic surface energy modifications to glass substrates can induce nucleation and improve crystallization outcomes for small molecule active pharmaceutical ingredients (APIs) and proteins. A comparatively broad probe for function is presented in which various APIs, proteins, organic solvents, aqueous media, surface energy motifs, crystallization methods, form factors, and flat and convex surface energy modifications were examined. Replicate studies (n ≥ 6) have demonstrated an average reduction in crystallization onset times of 52(4)% (alternatively 52 ± 4%) for acetylsalicylic acid from 91% isopropyl alcohol using two very different techniques: bulk cooling to 0 °C using flat surface energy modifications or microdomain cooling to 4 °C from the interior of a glass capillary having convex surface energy modifications that were immersed in the solution...
August 2, 2017: Crystal Growth & Design
https://www.readbyqxmd.com/read/28963158/antisepsis-of-the-skin-before-spinal-surgery-with-povidone-iodine-alcohol-followed-by-chlorhexidine-gluconate-alcohol-versus-povidone-iodine-alcohol-applied-twice-for-the-prevention-of-contamination-of-the-wound-by-bacteria-a-randomised-controlled-trial
#8
RANDOMIZED CONTROLLED TRIAL
S Patrick, A McDowell, A Lee, A Frau, U Martin, E Gardner, G McLorinan, N Eames
AIMS: The aim of this study was to determine whether the sequential application of povidone iodine-alcohol (PVI) followed by chlorhexidine gluconate-alcohol (CHG) would reduce surgical wound contamination to a greater extent than PVI applied twice in patients undergoing spinal surgery. PATIENTS AND METHODS: A single-centre, interventional, two arm, parallel group randomised controlled trial was undertaken, involving 407 patients who underwent elective spinal surgery...
October 2017: Bone & Joint Journal
https://www.readbyqxmd.com/read/28952727/cyanido-bridged-ln-iii-w-v-heterobinuclear-complexes-synthesis-and-magneto-structural-study
#9
Maria-Gabriela Alexandru, Diana Visinescu, Sergiu Shova, Francesc Lloret, Miguel Julve
A new series of cyanido-bridged {Ln(III)W(V)} heterobinuclear complexes of formula [Ln(III)(pyim)2(i-PrOH)(H2O)2(μ-CN)W(V)(CN)7]·2H2O [Ln = Gd (1), Tb (2), Dy (3), Ho (4), and Er (5); pyim = 2-(1H-imidazol-2-yl)-pyridine) and i-PrOH = isopropyl alcohol] were synthesized by one-pot reaction between (NH3Bu)3[W(CN)8] and [Ln(pyim)2](2+) complexes (generated in situ by mixing the corresponding Ln(III) ions and the pyim ligand). Compounds 1-5 are isomorphous and crystallize in the monoclinic system P21/n space group...
September 27, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28946506/the-effect-of-ultrasonic-vibration-and-surfactant-additive-on-fabrication-of-53-5gr-mm-silicon-echelle-grating-with-low-surface-roughness-in-alkaline-koh-solution
#10
Qingbin Jiao, Chunlin Zhu, Xin Tan, Xiangdong Qi, Bayanheshig
In the silicon echelle grating fabrication process, the "pseudo-mask" formed by the hydrogen bubbles generated during the etching process is the reason causing high surface roughness and poor surface quality of blazed plane. Based upon the ultrasonic mechanical effect and contact angle reduced by surfactant additive, ultrasonic vibration, isopropyl alcohol (IPA) and 2,4,7,9-Tetramethyl-5-decyne-4,7-diol (TMDD) were used to improve surface quality of 53.5gr/mm echelle grating. The surface roughness Rq is smaller than 18nm, 7nm and 2nm when using ultrasonic vibration, IPA and TMDD respectively...
January 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28927272/nonaqueous-sol-gel-synthesis-of-anatase-nanoparticles-and-their-electrophoretic-deposition-in-porous-alumina
#11
Cédric Frantz, Alessandro Lauria, Cristina V Manzano, Carlos Guerra-Nuñez, Markus Niederberger, Cédric Storrer, Johann Michler, Laetitia Philippe
Titanium dioxide (TiO2) nanoparticles were synthesized by nonaqueous sol-gel route using titanium tetrachloride and benzyl alcohol as the solvent. The obtained 4 nm-sized anatase nanocrystals were readily dispersible in various polar solvents allowing for simple preparation of colloidal dispersions in water, isopropyl alcohol, dimethyl sulfoxide, and ethanol. Results showed that dispersed nanoparticles have acidic properties and exhibit positive zeta-potential which is suitable for their deposition by cathodic electrophoresis...
October 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28913006/uhplc-pda-assay-for-simultaneous-determination-of-vitamin-d3-and-menaquinone-7-in-pharmaceutical-solid-dosage-formulation
#12
Muhammad Jehangir, Mahmood Ahmed, Muhammad Imtiaz Shafiq, Abdul Samad, Iftikhar-Ul-Haq
A newly developed method based on ultrahigh performance liquid chromatography (UHPLC) was optimized for the simultaneous determination of vitamin D3 and menaquinone-7 (MK-7) in tablet formulation in the present study. UHPLC separation of vitamin D3 and MK-7 was performed with ACE Excel 2 C18-PFP column (2 μm, 2.1 × 100 mm) at 0.6 mL min(-1) flow rate, whereas the mobile phase consisted of methanol/water (19 : 1, v/v, phase A) and isopropyl alcohol (99.9%, phase B) containing 0.5% triethylamine. Isocratic separation of both the analytes was performed at 40°C by pumping the mobile phases A and B in the ratio of 50 : 50 (v/v, pH, 6...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28905380/enhancing-blood-donor-skin-disinfection-using-natural-oils
#13
Meshari Alabdullatif, Imen Boujezza, Mohamed Mekni, Mariam Taha, Dilini Kumaran, Qi-Long Yi, Ahmed Landoulsi, Sandra Ramirez-Arcos
BACKGROUND: Effective donor skin disinfection is essential in preventing bacterial contamination of blood components with skin flora bacteria like Staphylococcus epidermidis. Cell aggregates of S. epidermidis (biofilms) are found on the skin and are resistant to the commonly used donor skin disinfectants chlorhexidine-gluconate and isopropyl alcohol. It has been demonstrated that essential oils synergistically enhance the antibacterial activity of chlorhexidine-gluconate. The objective of this study was to test plant-extracted essential oils in combination with chlorhexidine-gluconate or chlorhexidine-gluconate plus isopropyl alcohol for their ability to eliminate S...
September 14, 2017: Transfusion
https://www.readbyqxmd.com/read/28903829/quantification-of-induced-hypothermia-from-aseptic-scrub-applications-during-rodent-surgery-preparation
#14
Anna M Skorupski, Jingyi Zhang, Danielle Ferguson, Frank Lawrence, F Claire Hankenson
Laboratory mice (Mus musculus) are prone to develop hypothermia during anesthesia for surgery, thus potentially impeding anesthetic recovery, wound healing, and future health. The core body temperatures of isoflurane-anesthetized mice are influenced by the choice of supplemental heat sources; however, the contribution of various surgical scrubs on the body temperatures of mice under gas anesthesia has not been assessed. We sought to quantify the effect of using alcohol (70% isopropyl alcohol [IPA]) compared with saline to rinse away surgical scrub on the progression of hypothermia in anesthetized mice (n = 47)...
September 1, 2017: Journal of the American Association for Laboratory Animal Science: JAALAS
https://www.readbyqxmd.com/read/28876907/one-step-production-of-amphiphilic-nanofibrillated-cellulose-using-a-cellulose-producing-bacterium
#15
Kenji Tajima, Ryo Kusumoto, Ryota Kose, Hiroyuki Kono, Tokuo Matsushima, Takuya Isono, Takuya Yamamoto, Toshifumi Satoh
Nanofibrillated bacterial cellulose (NFBC) is produced by culturing a cellulose-producing bacterium (Gluconacetobacter intermedius NEDO-01) with rotation or agitation in medium supplemented with carboxymethylcellulose (CMC). Despite a high yield and dispersibility in water, the product immediately aggregates in organic solvents. To broaden its applicability, we prepared amphiphilic NFBC by culturing strain NEDO-01 in medium supplemented with hydroxyethylcellulose or hydroxypropylcellulose instead of CMC. Transmission electron microscopy analysis revealed that the resultant materials (HE-NFBC and HP-NFBC, respectively) comprised relatively uniform fibers with diameters of 33 ± 7 and 42 ± 8 nm, respectively...
September 19, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28865738/yeasticidal-activity-of-chemical-disinfectants-and-antiseptics-against-candida-auris
#16
G Moore, S Schelenz, A M Borman, E M Johnson, C S Brown
Candida auris appears to be transmitted readily between patients, yet information regarding the efficacy of environmental disinfection and skin decolonization is lacking. A quantitative suspension test (EN 13624:2013) was used to evaluate the yeasticidal activity of different chemical disinfectants and antiseptics against C. auris and Candida albicans. When tested in suspension, both a chlorine-based disinfectant and iodine-based skin antiseptic were effective against C. auris, suggesting that their use could reduce environmental contamination and skin colonization, respectively, if applied appropriately...
September 1, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28856894/a-low-energy-route-to-dna-wrapped-carbon-nanotubes-via-replacement-of-bile-salt-surfactants
#17
Jason K Streit, Jeffrey A Fagan, Ming Zheng
DNA-wrapped carbon nanotubes are a class of bionano hybrid molecules that have enabled carbon nanotube sorting, controlled assembly, and biosensing and bioimaging applications. The current method of synthesizing these hybrids via direct sonication of DNA/nanotube mixtures is time-consuming and not suitable for high-throughput synthesis and combinatorial sequence screening. Additionally, the direct sonication method does not make use of nanotubes presorted by extensively developed surfactant-based methods, is not effective for large diameter (>1 nm) tubes, and cannot maintain secondary and tertiary structural and functional domains present in certain DNA sequences...
September 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28831284/effect-of-isopropyl-alcohol-concentration-and-etching-time-on-wet-chemical-anisotropic-etching-of-low-resistivity-crystalline-silicon-wafer
#18
Eyad Abdur-Rahman, Ibrahim Alghoraibi, Hassan Alkurdi
A micropyramid structure was formed on the surface of a monocrystalline silicon wafer (100) using a wet chemical anisotropic etching technique. The main objective was to evaluate the performance of the etchant based on the silicon surface reflectance. Different isopropyl alcohol (IPA) volume concentrations (2, 4, 6, 8, and 10%) and different etching times (10, 20, 30, 40, and 50 min) were selected to study the total reflectance of silicon wafers. The other parameters such as NaOH concentration (12% wt.), the temperature of the solution (81...
2017: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/28796919/evaluation-of-a-needle-disinfectant-technique-to-reduce-infection-related-hospitalisation-after-transrectal-prostate-biopsy
#19
Gregory B Auffenberg, Ji Qi, Yuqing Gao, David C Miller, Zaojun Ye, Andrew Brachulis, Susan Linsell, Tejal N Gandhi, David Kraklau, James E Montie, Khurshid R Ghani
OBJECTIVES: To determine whether a needle disinfectant step during transrectal ultrasonography (TRUS)-guided prostate biopsy is associated with lower rates of infection-related hospitalisation. PATIENTS AND METHODS: We conducted a retrospective analysis of all TRUS-guided prostate biopsies taken across the Michigan Urological Surgery Improvement Collaborative (MUSIC) from January 2012 to March 2015. Natural variation in technique allowed us to evaluate for differences in infection-related hospitalisations based on whether or not a needle disinfectant technique was used...
August 10, 2017: BJU International
https://www.readbyqxmd.com/read/28795792/lu2-c82-nanorods-with-enhanced-photoluminescence-and-photoelectrochemical-properties
#20
Wangqiang Shen, Li Zhang, Shushu Zheng, Yunpeng Xie, Xing Lu
One-dimensional (1D) single-crystalline hexagonal nanorods of Lu2@C3v(8)-C82 were prepared for the first time using the liquid-liquid interface precipitation (LLIP) method from the interfaces between carbon disulfide (CS2) and isopropyl alcohol (IPA). The length of the nanorods can be readily controlled by varying the concentration of the Lu2@C82 solution in addition to the volume ratio of CS2 to IPA. The latter factor also exhibits a significant influence on the morphology of the crystals. The crystalline structure of the nanorods has been investigated by XRD and selected area electron diffraction (SAED), suggesting a face-centered cubic structure...
August 30, 2017: ACS Applied Materials & Interfaces
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