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nanoparticles uptake

Petra Cvjetko, Mira Zovko, Petra Peharec Štefanić, Renata Biba, Mirta Tkalec, Ana-Marija Domijan, Ivana Vinković Vrček, Ilse Letofsky-Papst, Sandra Šikić, Biljana Balen
The small size of nanoparticles (NPs), with dimensions between 1 and 100 nm, results in unique chemical and physical characteristics, which is why they are implemented in various consumer products. Therefore, an important concern is the potential detrimental impact of NPs on the environment. As plants are a vital part of ecosystem, investigation of the phytotoxic effects of NPs is particularly interesting. This study investigated the potential phytotoxicity of silver nanoparticles (AgNPs) on tobacco (Nicotiana tabacum) plants and compared it with the effects of the same AgNO3 concentrations...
December 8, 2017: Environmental Science and Pollution Research International
Jiaming Hu, Yan Sheng, Junfeng Shi, Bohao Yu, Zhiqiang Yu, Guochao Liao
The major limitation in the improving polymeric nanoparticles into an efficient gene/drug delivery carrier is the rapid opsonization, phagocytic uptake by mononuclear phagocyte system and subsequent clearance from the bloodstream. The prolonged circulation time of nanoparticles in the blood is a prerequisite to realizing a controlled and targeted (passive or active targeting) release of the encapsulated gene/drug at the desired site of action. In this review, the factors such as biological barriers and physical barriers including particle size, shape, zeta potential, and hydrophilicity will be discussed, which can cause effects on blood clearance and organ accumulation...
December 7, 2017: Current Drug Metabolism
Zhuan Li, Miao Liu, Li Ke Chen, Guang Zhu Li
OBJECTIVE: This paper aims to elucidate the combined toxicity of magnetite nanoparticles/Chromium [MNPs/Cr(VI)] adducts. METHODS: The HEK293 cell was exposed to either Cr(VI) or MNPs, or their adducts MNPs/Cr(VI). The cytotoxicity was evaluated by assessing the cell viability, apoptosis, oxidative stress induction, and cellular uptake. RESULTS: The toxicity of formed adducts is significantly reduced when compared to Cr(VI) anions. We found that the cellular uptake of MNPs/Cr(VI) adduct was rare, only few particles were endocytosed from the extracellular fluid and not accumulated in the cell nucleus...
November 2017: Biomedical and Environmental Sciences: BES
Apurva Badkas, Evan Frank, Zilan Zhou, Mina Jafari, Harish Chandra, Vishnu Sriram, Joo-Youp Lee, Jagjit S Yadav
There is an increasing interest in engineered nanoparticle (NP) conjugates for targeted and controlled drug delivery. However, the practical applications of these NP delivery vehicles remain constrained because of their reactivity with the body's immune system defenses resulting in undesirable off-target effects. In this study, poly(D,L lactide-co-glycolide) (PLGA)-b-polyethylene glycol (PEG) NPs conjugated to different quantities of the commercial antibody Herceptin® meant to target HER2-positive breast cancer cells were studied for their immune cell uptake and immunogenic properties (using murine macrophages and human dendritic cells)...
December 4, 2017: Colloids and Surfaces. B, Biointerfaces
Xiao Kuang, Shuang Zhou, Weiling Guo, Zhenjie Wang, Yanhui Sun, Hongzhuo Liu
Aminoglycoside-induced hearing loss stems from damage or loss of mechanosensory hair cells in the inner ear. Intrinsic mitochondrial cell death pathway plays a key role in that cellular dysfunction for which no proven effective therapies against oto-toxicities exist. Therefore, the aim of the present study was to develop a new mitochondrial targeting drug delivery system (DDS) that provided improved protection from gentamicin. Particularly, SS-31 peptide-conjugated geranylgeranylacetone (GGA) loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles were constructed successfully via emulsion-solvent evaporation method...
November 2017: Drug Delivery
Yuqing Gong, Pallabita Chowdhury, Narasimha M Midde, Mohammad A Rahman, Murali M Yallapu, Santosh Kumar
Purpose: Monocytes serve as sanctuary sites for HIV-1 from which virus is difficult to be eliminated. Therefore, an effective viral suppression in monocytes is critical for effective antiretroviral therapy (ART). This study focuses on a new strategy using nanoformulation to optimize the efficacy of ART drugs in HIV-infected monocytes. Methods: Poly(lactic-co-glycolic acid) (PLGA)-based elvitegravir nanoparticles (PLGA-EVG) were prepared by nano-precipitation technique...
December 2017: Biochemistry and Biophysics Reports
Fengshan Shen, Je-Kyun Park
To accurately assess potential nanotoxicity on the basis of cellular iron content, the precise separation of cells into subpopulations according to their magnetic nanoparticle loading is of crucial importance. In this study, we developed a microfluidic magnetophoresis device consisting of a trapezoidal channel containing five side outlet branches and a narrow rectangular channel with three outlet branches. This unique structure enabled the sequential separation of cells loaded with tiny amounts of iron oxide and cells heavily labeled with iron oxide, in a single device...
December 6, 2017: Analytical Chemistry
Mohamad Gabriel Alameh, Marc Lavertu, Nicolas Tran-Khanh, Chi-Yuan Chang, Frédéric Lesage, Martine Bail, Vincent Darras, Anik Chevrier, Michael D Buschmann
Chitosan (CS) shows in vitro and in vivo efficacy for siRNA delivery, but with contradictory findings for incompletely characterized systems. To understand which parameters produce effective delivery, a library of precisely characterized chitosans was produced at different DDAs and Mn. Encapsulation and transfection efficiencies were characterized in vitro. Formulations were selected to examine the influence of Mn and N:P ratio on nanoparticle uptake, metabolic activity, genotoxicity, and in vitro transfection...
December 6, 2017: Biomacromolecules
Ruming Jiang, Long Huang, Meiying Liu, Fengjie Deng, Hongye Huang, Jianwen Tian, Yuanqing Wen, Qian-Yong Cao, Xiaoyong Zhang, Yen Wei
The fabrication and biomedical applications of fluorescent polymeric nanoparticles (FPNs) with aggregation-induced emission (AIE) feature has attracted the most intensive research interest since the first discovery of AIE phenomenon by Tang' group. Although great attention has been devoted to preparation of AIE-active FPNs, an efficient, facile and versatile strategy is still highly desirable to advance their biomedical applications. In this work, a one-pot microwave-assisted multicomponent tandem polymerization was proposed to fabricate AIE-active FPNs based on a microwave-assisted Kabachnik-Fields (KF) reaction, which involves the conjugation of aldehyde group containing polyethylene glycol (CHO-PEG-CHO) and amino-group terminating AIE dye (H2N-PhE-NH2) using diethyl phosphate as the lock...
February 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Stefany Y Holguin, Michael D Gray, Princeton Joseph, Naresh N Thadhani, Mark R Prausnitz
Exposure of carbon-black (CB) nanoparticles to near-infrared nanosecond-pulsed laser energy can cause efficient intracellular delivery of molecules by photoporation. Here, cellular bioeffects of multi-walled carbon nanotubes (MWCNTs) and single-walled carbon nanotubes (SWCNTs) are compared to those of CB nanoparticles. In DU145 prostate-cancer cells, photoporation using CB nanoparticles transitions from (i) cells with molecular uptake to (ii) nonviable cells to (iii) fragmented cells with increasing laser fluence, as seen previously...
December 4, 2017: Advanced Healthcare Materials
Lester Thoo, Mochamad Z Fahmi, Ihsan N Zulkipli, Natasha Keasberry, Adi Idris
Carbon dot (Cdot) nanoparticles are an emerging class of carbon nanomaterials with a promising potential for drug delivery and bio imaging applications. Although the interaction between Cdots and non-immune cell types has been well studied, Cdot interactions with macrophages have not been investigated. Exposure of Cdot nanoparticles to J774.1 cells, a murine macrophage cell line, resulted in minimal toxicity, where notable toxicity was only seen with Cdot concentrations higher than 0.5 mg/ml. Flow cytometric analysis revealed that Cdots prepared from citric acid were internalized at significantly higher levels by macrophages compared with those prepared from bamboo leaves...
2017: Central-European Journal of Immunology
Kareem Ebeid, Xiangbing Meng, Kristina W Thiel, Anh-Vu Do, Sean M Geary, Angie S Morris, Erica L Pham, Amaraporn Wongrakpanich, Yashpal S Chhonker, Daryl J Murry, Kimberly K Leslie, Aliasger K Salem
Uterine serous carcinoma, one of the most aggressive types of endometrial cancer, is characterized by poor outcomes and mutations in the tumour suppressor p53. Our objective was to engender synthetic lethality to paclitaxel (PTX), the frontline treatment for endometrial cancer, in tumours with mutant p53 and enhance the therapeutic efficacy using polymeric nanoparticles (NPs). First, we identified the optimal NP formulation through comprehensive analyses of release profiles and cellular-uptake and cell viability studies...
December 4, 2017: Nature Nanotechnology
Sheldon Berke, Anne-Larissa Kampmann, Melinda Wuest, Justin J Bailey, Britta Glowacki, Frank Wuest, Klaus Jurkschat, Ralf Weberskirch, Ralf Schirrmacher
Nanoparticles represent the most widely studied drug delivery systems targeting cancer. Polymeric nanoparticles can be easily generated through a microemulsion polymerization. Herein, the synthesis, radiolabeling, and in vivo evaluation of nanoparticles (NPs) functionalized by an organosilicon fluoride acceptor (SiFA) are reported which can be radiolabeled without further chemical modifications. Four nanoparticles in the sub-100 nm range with distinct hydrodynamic diameters of 20 nm (NP1), 33 nm (NP2), 45 nm (NP3), and 72 nm (NP4), respectively, were synthesized under size-controlled conditions...
December 4, 2017: Bioconjugate Chemistry
Tianshu Wu, Meng Tang
Despite the increasing number of neurotoxicological studies on metal-containing nanoparticles (NPs), the NP-induced neuroinflammation has not yet been well understood. This review provides a comprehensive understanding of inflammatory responses to two typical metal-containing NPs, namely silver NPs (Ag-NPs) and titanium dioxide NPs (TiO2-NPs). Ag-NPs and TiO2-NPs could translocate into the CNS through damaged blood-brain barrier, nerve afferent signaling and eye-to-brain ways, and even cell uptake. NPs could stimulate the activation of glial cells to release proinflammatory cytokines and generate reactive oxygen species and nitric oxide production, resulting in the neuroinflammation...
December 4, 2017: Nanomedicine
A A Lopera, A Montoya, I D Vélez, S M Robledo, C P Garcia
The traditional drugs used in the treatment of cutaneous leishmanisis (CL) have multiple disadvantages, such as high toxicity, high costs, and more recently the appearance of parasites resistant to those drugs. For this reason, some research has focused on the development of new drugs or treatment therapies. Photodynamic therapy (PDT) that involves the use of a photosensitive or photosensitizing compound capable of producing reactive oxygen species to which Leishmania parasites are sensitive, has emerged as an alternative for the treatment of CL...
November 30, 2017: Photodiagnosis and Photodynamic Therapy
Jordan A Pitt, Jordan S Kozal, Nishad Jayasundara, Andrey Massarsky, Rafael Trevisan, Nick Geitner, Mark Wiesner, Edward D Levin, Richard T Di Giulio
Plastic pollution is a critical environmental concern and comprises the majority of anthropogenic debris in the ocean, including macro, micro, and likely nanoscale (less than 100nm in at least one dimension) plastic particles. While the toxicity of macroplastics and microplastics is relatively well studied, the toxicity of nanoplastics is largely uncharacterized. Here, fluorescent polystyrene nanoparticles (PS NPs) were used to investigate the potential toxicity of nanoplastics in developing zebrafish (Danio rerio), as well as to characterize the uptake and distribution of the particles within embryos and larvae...
November 24, 2017: Aquatic Toxicology
Pilar de la Puente, Micah J Luderer, Cinzia Federico, Abbey Jin, Rebecca C Gilson, Christopher Egbulefu, Kinan Alhallak, Shruti Shah, Barbara Muz, Jennifer Sun, Justin King, Daniel Kohnen, Noha Nabil Salama, Samuel Achilefu, Ravi Vij, Abdel Kareem Azab
The establishment of more effective treatments that can circumvent chemoresistance in Multiple Myeloma (MM) is a priority. Although bortezomib (BTZ) is one of the most potent proteasome inhibitors available, still possesses limitations related to dose limiting side effects. Several strategies have been developed to improve the delivery of chemotherapies to MM by targeting different moieties expressed on MM cells to nanoparticle delivery systems (NPs), which have failed mainly due to their heterogeneous expression on these cells...
November 28, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
Surya Prakash Singh, Syed Baseeruddin Alvi, Deepak Bharadwaj, Anula Divyash Singh, Sasidhar Venkata Manda, Rohit Srivastava, Aravind Kumar Rengan
We report the synthesis of a biodegradable liposome gold nanoparticles for curcumin (Au-Lipos Cur NPs) delivery. This entrapped curcumin served as an in situ adjuvant for photothermal therapy. Curcumin was loaded in Au-Lipos NPs with an encapsulation efficiency of ∼70%. The gold coating enabled the NPs to specifically absorb NIR light (780nm) by virtue of Surface Plasmon Resonance (SPR) and this light energy was converted to heat. The generated heat destabilized the liposomal core enhancing the release of encapsulated curcumin...
November 28, 2017: International Journal of Biological Macromolecules
Christopher R Anderson, Yehou D M Gnopo, Filippo Gambinossi, Steven E Mylon, James K Ferri
Nanoparticle (NP) interactions with cellular systems are influenced by both NP physico-chemical properties and the presence of surface-bound proteins that are adsorbed in biological environments. Here, we characterize cellular responses to silver nanoparticles (AgNPs) functionalized with poly(MeO2 MAx -co-OEGMAy ) brushes with tunable hydrophobicity and explore how these responses are modulated by the presence or absence of serum proteins. Poly(MeO2 MAx -co-OEGMAy ) with variable composition (5-10% OEGMA) was fabricated to elicit differential hydrophobicity at 37°C for AgNPs capped with these copolymers...
November 30, 2017: Journal of Biomedical Materials Research. Part A
Shane Cheung, Donal F O'Shea
Directed self-assemblies in water are known as the most efficient means of forming complex higher ordered structures in nature. Here we show a straightforward and robust method for particle assembly which utilises the amphiphilic tri-block co-polymer poloxamer-188 and a hydrophobic fluorophore as the two designer components, which have a built-in ability to convey spatial and temporal information about their surroundings to an observer. Templating of particle self-assembly is attributed to interactions between the fluorophore and hydrophobic segment of the poloxamer...
December 1, 2017: Nature Communications
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