keyword
MENU ▼
Read by QxMD icon Read
search

Cryopreservation

keyword
https://www.readbyqxmd.com/read/29341292/amniotic-fluid-stem-cell-exosomes-therapeutic-perspective
#1
Francesca Beretti, Manuela Zavatti, Francesca Casciaro, Giuseppina Comitini, Fabrizia Franchi, Veronica Barbieri, Giovanni B La Sala, Tullia Maraldi
It is widely accepted that the therapeutic potential of stem cells can be largely mediated by paracrine factors, also included into exosomes. Thus, stem cell-derived exosomes represent a major therapeutic option in regenerative medicine avoiding, if compared to stem cells graft, abnormal differentiation and tumor formation. Exosomes derived from mesenchymal stem cells (MSC) induce damaged tissue repair, and can also exert immunomodulatory effects on the differentiation, activation and function of different lymphocytes...
January 16, 2018: BioFactors
https://www.readbyqxmd.com/read/29338854/long-term-outcomes-in-cancer-patients-who-did-or-did-not-pursue-fertility-preservation
#2
Molly B Moravek, Rafael Confino, Kristin N Smith, Ralph R Kazer, Susan C Klock, Angela K Lawson, William J Gradishar, Mary Ellen Pavone
OBJECTIVE: To compare long-term outcomes of cancer patients who pursued fertility preservation (FP) with those who did not and compare random-start (RS) and menstrual cycle-specific (CS) protocols for FP. DESIGN: Retrospective cohort. SETTING: Single urban academic institution. PATIENT(S): Oncology patients who contacted the FP patient navigator, 2005-2015. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Time to cancer treatment, disease-free survival, and reproductive outcomes in FP versus no-FP patients and cycle outcomes for RS versus CS protocols...
January 12, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29338138/could-cryopreserved-human-semen-samples-be-stored-at-80%C3%A2-c
#3
Carlos R Vaz, Tamara Lamim, Rafael A Salvador, Anna P B Batschauer, Vera Lucia L Amaral, David Til
OBJECTIVE: To evaluate storage time effects in cryopreserved human semen samples, kept in the freezer at a controlled temperature of -80°C, on sperm viability after thawing. METHODS: We used 20 semen samples. Each sample was cryopreserved in 10 fingers, which were divided into five groups: one group was kept in cryogenic canisters throughout the experiment(control), and four groups were kept in a VIP Ultra Low MDF-U76V- PE freezer, with the temperature set at -80°C, for 24, 48, 72 and 96 hours, respectively...
January 17, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29337420/a-proinflammatory-response-of-bovine-endometrial-epithelial-cells-to-active-sperm-in-vitro
#4
Ahmed Essam Elweza, Mohamed Aboul Ezz, Tomas J Acosta, Anup K Talukder, Takashi Shimizu, Hiroyuki Hayakawa, Masayuki Shimada, Kazuhiko Imakawa, Ahmed Hamed Zaghloul, Akio Miyamoto
In the cow, cryopreserved semen is inseminated into the uterus, and most of sperm are removed by backflow and phagocytes. Nevertheless, the mechanism responsible for sperm phagocytosis is unclear. Here, we used cultured bovine uterine epithelial cells (BUECs) to investigate the uterine response to sperm and the mechanism that activates polymorphonuclear neutrophils (PMNs). BUEC monolayers were co-cultured with different numbers of washed sperm obtained from cryopreserved semen (104 , 105 and 106 sperm/ml) for 3 h...
January 16, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29334771/development-of-anti-human-mesothelin-targeted-chimeric-antigen-receptor-car-messenger-rna-mrna-transfected-peripheral-blood-lymphocytes-carma-hmeso-for-ovarian-cancer-therapy
#5
Chien-Fu Hung, Xuequn Xu, Linhong Li, Ying Ma, Qiu Jin, Angelia Viley, Cornell Allen, Pachai Natarajan, Rama Shivakumar, Madhusudan V Peshwa, Leisha A Emens
CD19-targeted chimeric antigen receptor (CAR) engineered T/natural kill (NK)-cell therapies can result in durable clinical responses in B-cell malignancies. However, CAR-based immunotherapies have been much less successful in solid cancers, in part due to 'on-target off-tumor' toxicity related to expression of target tumor antigens on normal tissue. Based on preliminary observations of safety and clinical activity in proof-of-concept clinical trials, tumor antigen-specific messenger RNA (mRNA) CAR transfection into selected, activated, and expanded T/NK-cells may permit prospective control of 'on-target off-tumor toxicity'...
January 15, 2018: Human Gene Therapy
https://www.readbyqxmd.com/read/29334209/comparison-of-the-effects-of-vitrification-on-gene-expression-of-mature-mouse-oocytes-using-cryotop-and-open-pulled-straw
#6
Fardin Amidi, Zahra Khodabandeh, Mohamad Hossain Nori Mogahi
Background: Oocyte cryopreservation is an essential part of the assisted reproductive technology (ART), which was recently introduced into clinical practice. This study aimed to evaluate the effects of two vitrification systems-Cryotop and Open Pulled Straw (OPS)-on mature oocytes gene expressions. MATERIALS AND METHODS: In this experimental study, the survival rate of metaphase II (MII) mouse oocytes were assessed after cryopreservation by vitrification via i. OPS or ii...
April 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29334034/improved-exogenous-dna-uptake-in-bovine-spermatozoa-and-gene-expression-in-embryos-using-membrane-destabilizing-agents-in-icsi-smgt
#7
Esther Sánchez-Villalba, María Elena Arias, Fabiola Zambrano, Pía Loren, Ricardo Felmer
Sperm-mediated gene transfer (SMGT) is a simple, fast, and economical biotechnological tool for producing transgenic animals. However, transgene expression with this technique in bovine embryos is still inefficient due to low uptake and binding of exogenous DNA in spermatozoa. The present study evaluated the effects of sperm membrane destabilization on the binding capacity, location and quantity of bound exogenous DNA in cryopreserved bovine spermatozoa using Triton X-100 (TX-100), lysolecithin (LL) and sodium hydroxide (NaOH)...
January 15, 2018: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29333568/comparison-of-effects-of-thawing-entire-donor-sperm-vial-vs-partial-thawing-shaving-on-sperm-quality
#8
Micha Baum, Raoul Orvieto, Sapir Kon, Ronit Machtinger, Gil M Yerushalmi, Ariel Hourvitz
PURPOSE: Partial thawing of a vial of cryopreserved sperm (shaving) is sometimes applied as a measure to preserve sperm for further use, particularly in cases of very restricted sperm quantity. However, mechanical violence may disrupt the sperm-wall and lead to impaired in-vitro fertilization (IVF) outcomes. MATERIAL AND METHODS: In a retrospective case-control study at a tertiary, university-affiliated medical center, we compared the IVF/intracytoplasmic sperm injection (ICSI) outcomes of patients who used donor sperm following partial thawing (shaving) of the vial of cryopreserved sperm (n = 99) to a control group consisting of patients for whom the vial of sperm was completely thawed before use (n = 99)...
January 15, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29333408/is-long-term-storage-of-cryopreserved-stem-cells-for-hematopoietic-stem-cell-transplantation-a-worthwhile-exercise-in-developing-countries
#9
Santhosh Kumar Devadas, Minal Khairnar, Sumathi S Hiregoudar, Shashank Ojha, Sachin Punatar, Alok Gupta, Anant Gokarn, Pallavi Bhole, Sadhana Kannan, Navin Khattry
Background: Stem cell units (SCUs) that are cryopreserved prior to both autologous and allogeneic hematopoietic stem cell transplants (for donor lymphocyte infusion) remain unused or partially used several times, and become an increased burden to blood banks/SCU repositories. Because of the scarcity of data regarding the duration for which the storage is useful, there is no general consensus regarding disposal of SCUs. Methods: We conducted a retrospective audit of SCU utilization in 435 patients who planned to undergo either autologous stem cell transplantation (auto-SCT) (N=239) or allogeneic stem cell transplantation (allo-SCT) (N=196) at a tertiary cancer care center between November 2007 to January 2015...
December 2017: Blood Research
https://www.readbyqxmd.com/read/29331832/investigations-on-a-cryopreservation-protocol-for-long-term-storage-of-psittacine-spermatozoa-using-cockatiel-semen-as-an-example
#10
H Schneider, D Fischer, K Failing, C Ehling, S Meinecke-Tillmann, A Wehrend, M Lierz
The aim of the present study was the establishment of an effective protocol for cryopreservation of psittacine semen. Therefore, pooled semen samples of 30 cockatiels (Nymphicus hollandicus) were diluted with modified Lake diluent (1:4), partitioned into four equal parts. Three portions were mixed with three cryoprotectants (dimethylacetamide, dimethyl sulfoxide, glycerol) in 4%, 8% and 12% final concentration, respectively, whereas the 4rth part served as control. Altogether, 96 incremental diluted semen samples were obtained for investigation...
December 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/29331828/a-recovery-time-after-warming-restores-mitochondrial-function-and-improves-developmental-competence-of-vitrified-ovine-oocytes
#11
Sara Succu, Sergio Domenico Gadau, Elisa Serra, Angelo Zinellu, Ciriaco Carru, Cristian Porcu, Salvatore Naitana, Fiammetta Berlinguer, Giovanni Giuseppe Leoni
The aim of the present study was to assess the ability of vitrified/warmed oocyte to recover from vitrification-induced damages after warming. In vitro matured, vitrified/warmed ovine oocytes were assessed for developmental competence, mitochondrial activity and distribution, ATP, ROS and catalase levels during 6 h of in vitro culture using fresh oocytes as control. ATP content in vitrified oocytes was lower than control during 4 h of post warming culture (p < .01). Vitrified oocytes were able to fill this gap only after 6 h of post-warming incubation...
December 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/29331236/pregnancy-related-complications-and-perinatal-outcomes-resulting-from-transfer-of-cryopreserved-versus-fresh-embryos-in%C3%A2-vitro-fertilization-a-meta-analysis
#12
Tingting Sha, Xunqiang Yin, Wenwei Cheng, Isaac Yaw Massey
OBJECTIVE: To provide an updated comparison of pregnancy-related complications and adverse perinatal outcomes of pregnancies conceived after frozen embryo transfer (FET) versus fresh embryo transfer (fresh ET). DESIGN: Meta-analysis. SETTING: University. PATIENT(S): Pregnancies resulting from FET versus fresh ET. INTERVENTIONS(S): Pubmed, Embase, Cochrane Library, Google Scholar, and Chinese databases, including the China National Knowledge Infrastructure Database, Wanfang, and Chinese Scientific Journals Full-Text Database were searched by two independent reviewers from January 1980 to September 2017...
January 11, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29330877/characterization-of-biologic-response-modifiers-in-the-supernatant-of-conventional-refrigerated-and-cryopreserved-platelets
#13
Lacey Johnson, Shereen Tan, Emily Jenkins, Ben Wood, Denese C Marks
BACKGROUND: Alternatives to room temperature storage of platelets (PLTs) are of interest to support blood banking logistics. The aim of this study was to compare the presence of biologic response modifiers (BRMs) in PLT concentrates stored under conventional room temperature conditions with refrigerated or cryopreserved PLTs. STUDY DESIGN AND METHODS: A three-arm pool-and-split study was carried out using buffy coat-derived PLTs stored in 30% plasma/70% SSP+. The three matched treatment arms were as follows: room temperature (20-24°C), cold (2-6°C), and cryopreserved (-80°C with DMSO)...
January 12, 2018: Transfusion
https://www.readbyqxmd.com/read/29330637/assessment-of-the-permeability-properties-of-cryopreservation-outer-bags-used-in-nhsbt
#14
P Hogg, P Rooney, R Lomas, J N Kearney
This study was carried out to investigate leakage/transport across the bag material of six outer cryopreservation bags in common use within NHS Blood and Transplant. In order to do this two different leak testing procedures; coloured dye and hydrogen tracer gas, were used. The data obtained show that a coloured dye cannot permeate through the materials both at room temperature and following storage at liquid nitrogen temperature (- 196 °C). In addition, when filled with the smallest elemental molecule, hydrogen, in the form of a tracer gas, all of the bags only allowed trace amounts of hydrogen to escape, either through the seal or the bag material...
January 12, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29329425/comparison-of-hepatic-2d-sandwich-cultures-and-3d-spheroids-for-long-term-toxicity-applications-a-multi-center-study
#15
Catherine C Bell, Anita C A Dankers, Volker M Lauschke, Rowena Sison-Young, Roz Jenkins, Cliff Rowe, Chris Goldring, Kevin Park, Sophie Regan, Tracy Walker, Chris Schofield, Audrey Baze, Alison J Foster, Dominic Williams, Amy W M van de Ven, Frank Jacobs, Jos van Houdt, Tuula Lähteenmäki, Jan Snoeys, Satu Juhila, Lysiane Richert, Magnus Ingelman-Sundberg
Primary human hepatocytes (PHH) are commonly used for in vitro studies of drug-induced liver injury. However, when cultured as 2D monolayers, PHH lose crucial hepatic functions within hours. This dedifferentiation can be ameliorated when PHH are cultured in sandwich configuration (2Dsw), particularly when cultures are regularly re-overlaid with extracellular matrix, or as 3D spheroids. In this study, the six participating laboratories evaluated the robustness of these two model systems made from cryopreserved PHH from the same donors considering both inter-donor and inter-lab variability and compared their suitability for use in repeated-dose toxicity studies using 5 different hepatotoxins with different toxicity mechanisms...
January 10, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29329231/development-of-a-cytocompatible-scaffold-from-pig-immature-testicular-tissue-allowing-human-sertoli-cell-attachment-proliferation-and-functionality
#16
Maxime Vermeulen, Federico Del Vento, Francesca de Michele, Jonathan Poels, Christine Wyns
Cryopreservation of immature testicular tissue before chemo/radiotherapy is the only option to preserve fertility of cancer-affected prepubertal boys. To avoid reintroduction of malignant cells, development of a transplantable scaffold by decellularization of pig immature testicular tissue (ITT) able to support decontaminated testicular cells could be an option for fertility restoration in these patients. We, therefore, compared decellularization protocols to produce a cytocompatible scaffold. Fragments of ITT from 15 piglets were decellularized using three protocols: sodium dodecyl sulfate (SDS)-Triton (ST), Triton-SDS-Triton (TST) and trypsin 0...
January 12, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29327217/cryobiotechnology-of-apple-malus-spp-development-progress-and-future-prospects
#17
REVIEW
Min-Rui Wang, Long Chen, Jaime A Teixeira da Silva, Gayle M Volk, Qiao-Chun Wang
Cryopreservation provides valuable genes for further breeding of elite cultivars, and cryotherapy improves the production of virus-free plants in Malus spp., thus assisting the sustainable development of the apple industry. Apple (Malus spp.) is one of the most economically important temperate fruit crops. Wild Malus genetic resources and existing cultivars provide valuable genes for breeding new elite cultivars and rootstocks through traditional and biotechnological breeding programs. These valuable genes include those resistant to abiotic factors such as drought and salinity, and to biotic factors such as fungi, bacteria and aphids...
January 11, 2018: Plant Cell Reports
https://www.readbyqxmd.com/read/29325880/cryoprotective-effect-of-glutamine-taurine-and-proline-on-post-thaw-semen-quality-and-dna-integrity-of-donkey-spermatozoa
#18
M Bottrel, D Acha, I Ortiz, M Hidalgo, J Gósalvez, J Camisão, J Dorado
This study was conducted to evaluate the effect of amino acid addition to semen on post-thaw quality of donkey spermatozoa. Eighteen ejaculates were pooled and divided into aliquots which were cryopreserved in Gent A® containing 1% ethylene glycol (Gent-EG) and supplemented with 0 (as control), 20, 40, or 60 mM of glutamine, proline, or taurine. The greatest concentration (60 mM) of glutamine and taurine resulted in greater (P < 0.001) post-thaw sperm motility. Amino acid supplementation did not improve (P > 0...
December 30, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29322685/feasibility-safety-and-tolerance-of-mesenchymal-stem-cell-therapy-for-obstructive-chronic-lung-allograft-dysfunction
#19
Cesar Ariel Keller, Thomas Arthur Gonwa, David Orel Hodge, Derek Joseph Hei, John Michael Centanni, Abba Chedi Zubair
Feasibility, tolerance, and safety of intravenous infusions of allogeneic mesenchymal stem cell (MSC) therapy in lung transplant recipients with bronchiolitis obliterans syndrome (BOS) are not well established. MSCs were manufactured, cryopreserved, transported to our facility, thawed, and infused into nine recipients with moderate BOS (average drop in forced expiratory volume in 1 second was 56.8% ± 3.2% from post-transplant peak) who were refractory to standard therapy and not candidates for retransplant...
January 11, 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29322051/genetic-variation-of-the-%C3%AE-tubulin-gene-of-babesia-caballi-strains
#20
María Guadalupe Montes-Cortés, José Luis Fernández-García, Miguel Ángel Habela Martínez-Estéllez
Background: Equine piroplasmosis is caused by two haemoprotozoan parasites: Babesia caballi and Theileria equi. Negative economic impact on international trade has been associated to endemic sites. This is the reason why carrier detection requires reliable diagnostic methods. Various diagnostic modalities can be used alone or in combination including PCR. However, genetic variation of commonly used genes is still of debate. The aim of this research was to sequence the β-tubulin gene of a B...
September 2017: Journal of Arthropod-borne Diseases
keyword
keyword
11485
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"