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Cryopreservation

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https://www.readbyqxmd.com/read/27919790/preservation-of-malassezia-pachydermatis-using-skimmed-milk-as-cryoprotective-agent
#1
F A Silva Sergent, B Acosta-Hernández, J R Jaber, I Rosario Medina, F Real Valcárcel, D Padilla Castillo, S Déniz Suárez
This study evaluated the effect of skimmed milk as a cryoprotectant for the maintenance and long-term preservation of 70 Malassezia pachydermatis strains. An initial suspension of each strain was prepared in sterile distilled water with skimmed milk. The most effective method was cryopreservation that ensured 100% viability for 2years.
December 2, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27919572/non-fucosylated-cb-cd34-cells-represent-a-good-target-for-enforced-fucosylation-to-improve-engraftment-following-cord-blood-transplantation
#2
Simon N Robinson, Michael W Thomas, Paul J Simmons, Junjun Lu, Hong Yang, Jeannie A Javni, Elizabeth J Shpall, Patrick A Zweidler-Mckay
BACKGROUND AIMS: Despite ethnic diversity and ready availability of cryopreserved, human leukocyte antigen-typed cord blood (CB), delayed engraftment remains a significant hurdle to successful CB transplantation. Suboptimal homing of CB hematopoietic stem and progenitor cells (HSPCs) to the hematopoietic microenvironment (HM) is thought to be responsible and due to low levels of HSPC fucosylation. Fucosylation (decoration with sialyl-Lewis(X)) may improve HSPC homing to HM by increasing the strength of HSPC/E-selectin interactions, where E-selectin is constitutively expressed by HM microvasculature...
December 2, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27919288/variation-in-male-spermiation-response-to-exogenous-hormones-among-divergent-populations-of-red-eyed-treefrogs
#3
Leah E Jacobs, Jeanne M Robertson, Kristine Kaiser
BACKGROUND: The non-lethal collection of sperm from live males is an important component for multiple captive-breeding techniques, including assisted reproductive technology (ART) protocols, sperm cryopreservation and in vitro fertilization. However, in amphibians, the type and amount of hormone necessary to induce spermiation can be highly variable, even among closely related species. We are unaware of any studies that have examined the spermiation response to exogenous hormones across highly differentiated populations within a species...
December 5, 2016: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/27917673/-first-attempts-in-the-introduction-of-cryopreservation-of-ovarian-tissues
#4
Péter Fancsovits, János Urbancsek, László Fónyad, Anna Sebestyén, Gézáné Csorba, Ádám Lehner, Zita Kaszás, János Rigó, Attila Bokor
INTRODUCTION: The oncological treatment may damage ovarian function. To prevent this, it is possible to cryopreserve the ovarian tissue, and to keep the samples for long-term storage. The frozen-thawed tissue could be retransplanted after chemo- or radiotherapy. AIM: The aim of our study was to examine the effect of cryopreservation on the viability of ovarian tissue. METHOD: We analyzed the survival of frozen-thawed donated ovarian tissues...
December 2016: Orvosi Hetilap
https://www.readbyqxmd.com/read/27917125/comparison-of-liver-cell-models-using-the-basel-phenotyping-cocktail
#5
Benjamin Berger, Massimiliano Donzelli, Swarna Maseneni, Franziska Boess, Adrian Roth, Stephan Krähenbühl, Manuel Haschke
Currently used hepatocyte cell systems for in vitro assessment of drug metabolism include hepatoma cell lines and primary human hepatocyte (PHH) cultures. We investigated the suitability of the validated in vivo Basel phenotyping cocktail (caffeine [CYP1A2], efavirenz [CYP2B6], losartan [CYP2C9], omeprazole [CYP2C19], metoprolol [CYP2D6], midazolam [CYP3A4]) in vitro and characterized four hepatocyte cell systems (HepG2 cells, HepaRG cells, and primary cryopreserved human hepatocytes in 2-dimensional [2D] culture or in 3D-spheroid co-culture) regarding basal metabolism and CYP inducibility...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27916562/cryobiological-parameters-of-multipotent-stromal-cells-obtained-from-different-sources
#6
L Lauterboeck, W F Wolkers, B Glasmacher
Stem cells are important for regenerative medicine mainly due to their multilineage differentiation capacity. However, the cells rapidly loose this capability during culturing. Cryopreservation preserves the differentiation potential of the cells, until they are needed. In this study, specific cell properties of multipotent stromal cells (MSCs), from the common marmoset monkey Callithrix jacchus MSCs derived from amnion (Am) and bone marrow (Bm) were studied in order to predict optimal cooling rates for cryopreservation...
December 1, 2016: Cryobiology
https://www.readbyqxmd.com/read/27916207/integrating-insulin-into-single-step-culture-medium-regulates-human-embryo-development-in%C3%A2-vitro
#7
Mohamed Fawzy, Mohamed Sabry, Mohamed Nour, Mohamed Y Abdelrahman, Eman Roshdy, Yasmin Magdi, Hazem Abdelghafar
OBJECTIVE: To evaluate the effect of supplementing single-step embryo culture medium with insulin on human embryo development. DESIGN: Comparative study. SETTING: Two private centers. PATIENT(S): The study involved a sibling oocyte split of 5,142 retrieved oocytes from 360 patients. INTERVENTION(S): Sibling oocytes split after intracytoplasmic sperm injection for culture from day 0 through day 5 or 6 in insulin-supplemented or control medium...
December 1, 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27916206/how-many-oocytes-are-optimal-to%C3%A2-achieve-multiple-live-births-with%C3%A2-one-stimulation-cycle-the%C3%A2-one%C3%A2-and-done-approach
#8
Denis A Vaughan, Angela Leung, Nina Resetkova, Robin Ruthazer, Alan S Penzias, Denny Sakkas, Michael M Alper
OBJECTIVE: To study how many infertility patients would complete an average-sized family (achieve ≥2 live births) after a single, complete in vitro fertilization (IVF) cycle. DESIGN: A retrospective cohort study. SETTING: University-affiliated private infertility practice. PATIENT(S): Women undergoing IVF. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): The outcome of 1 or ≥2 live births after a single retrieval cycle, followed by use of all embryos in subsequent frozen cycles in relation to oocyte number...
December 1, 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27916095/-decreased-sirt1-expression-is-related-to-bronchopulmonary-dysplasia-in-premature-infants-after-oxygen-exposure
#9
Fengmei Tan, Wenbin Dong, Xiaoping Lei, Xingling Liu, Qingping Li, Lan Kang, Shuai Zhao, Chan Zhang
Objective To observe silent information regulator 1 (SIRT1) expression in the peripheral blood mononuclear cells (PBMCs), and explore the relationship between SIRT1 expression and bronchopulmonary dysplasia (BPD) in premature infants after oxygen exposure. Methods Premature infants with 28 to 30 weeks' gestation were divided into three groups according to the fraction of inspiration O2 (FiO2): low-oxygen group with FiO2<30%, medium-oxygen group with FiO2 being 30%~40%, and high-oxygen group with FiO2≥40%; other premature infants with 28 to 30 weeks' gestation and without inspiration O2 served as a control group...
December 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/27914736/research-progress-of-platelet-transfusion-in-china
#10
REVIEW
Qiushi Wang, Jiangcun Yang, Lori Stevens, Deqing Wang
Platelet products have been increasingly used for more than 50 years. Platelet transfusion is effective for correcting bleeding caused by thrombocytopenia and platelet function defects. In this review, we will outline research on platelet transfusion in China including platelet biosafety, cryopreservation of platelets, the assessment of the effectiveness of platelet transfusion, the causes of platelet transfusion refractoriness including immunization against CD36, and neonatal alloimmune thrombocytopenia.
November 27, 2016: Transfusion Medicine Reviews
https://www.readbyqxmd.com/read/27913779/comparison-of-proliferative-and-immunomodulatory-potential-of-adipose-derived-mesenchymal-stem-cells-from-young-and-geriatric-cats
#11
Lara B Zajic, Tracy L Webb, Polly Webb, Jonathan W Coy, Steve W Dow, Jessica M Quimby
OBJECTIVES: The objective of this study was to compare the ability of adipose-derived mesenchymal stem cells (aMSC) generated from young vs geriatric cats to proliferate in culture, suppress lymphocyte proliferation and undergo senescence. METHODS: Adipose tissues from five young (<5 years) and six geriatric (>10 years) cats were harvested and cryopreserved for subsequent aMSC isolation and culture. aMSC proliferation in culture was compared via determination of time until passage two and by 3-(4,5-demethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay...
December 2, 2016: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/27909569/the-walking-egg-project-how-to-start-a-twe-centre
#12
W Ombelet, J Goossens
The main objective of the Walking Egg non-profit organization is the implementation of good quality and affordable infertility centres in resource-poor countries. Three levels of assistance are suggested: A level 1 infertility clinic is a basic infertility clinic capable of offering semen analysis, hormonal assays, follicular scanning, ovulation induction and intrauterine inseminations. Level 2 infertility clinics are supposed to perform simplified IVF as well. Level 3 infertility clinics capable of offering ICSI, cryopreservation and operative endoscopy are not part of the Walking Egg Project in the initial phase...
June 27, 2016: Facts, Views & Vision in ObGyn
https://www.readbyqxmd.com/read/27908687/fertility-response-evaluation-of-artificial-insemination-methods-in-sheep-with-fresh-and-frozen-thawed-semen
#13
Reza Masoudi, Ahmad Zare Shahneh, Armin Towhidi, Hamid Kohram, Abbas Akbarisharif, Mohsen Sharafi
The aim of this study was to evaluate the fertility response of artificial insemination (AI) methods with fresh and frozen sperm in sheep. In experiment 1, one hundred and fifty fat tailed Zandi ewes were assigned into 3 equal groups and inseminated with three AI methods consisting of vaginal, laparoscopic and trans-cervical AI with fresh semen. In experiment 2, a factorial study (3 AI methods × 2 extenders) was used to analyze the effects of three AI methods and two freezing extenders containing soybean lecithin (SL) or Egg yolk (EY) on reproductive performance of 300 fat tailed Zandi ewes...
November 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27908686/effect-of-different-vitrification-solutions-and-cryodevices-on-viability-and-subsequent-development-of-buffalo-oocytes-vitrified-at-the-germinal-vesicle-gv-stage
#14
Amr S El-Shalofy, Adel R Moawad, Gamal M Darwish, Sayed T Ismail, Aly B A Badawy, Magdy R Badr
The cryopreservation of immature oocytes would generate a readily available, non-seasonal source of female gametes for research and reproduction. In domestic animals, the most promising results on oocyte cryopreservation have been reported in cattle, few studies have been conducted on buffalo. The aim of the present study was to compare the use of different vitrification solutions and various cryodevices on viability and developmental competence of buffalo oocytes vitrified at the germinal vesicle (GV) stage...
November 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27906588/fertility-preservation-in-pediatric-and-adolescent-oncology-patients-the-decision-making-process-of-parents
#15
Nancy Li, Yasmin Jayasinghe, Matthew A Kemertzis, Paddy Moore, Michelle Peate
PURPOSE: Decisions surrounding fertility preservation (FP) in children, adolescents, and adults can be difficult due to the distress of a cancer diagnosis, time constraints for decision-making, and lack of efficacy data. This review examines the decision-making process of oncology patients and their parents (if patients are in the pediatric or adolescent population) to better understand experiences of decisional conflict and regret. METHODS: Two electronic databases, Embase and Pubmed, were searched using the terms (Decision-making OR Conflict (Psychology) OR Decision regret) AND (Freezing OR Oocyte OR Ovarian tissue OR Semen preservation OR Fertility preservation OR Cryopreservation) AND (Neoplasms OR Cancer OR Chemotherapy OR Drug therapy OR Radiotherapy)...
December 1, 2016: Journal of Adolescent and Young Adult Oncology
https://www.readbyqxmd.com/read/27904170/twenty-five-year-old-woman-with-bilateral-borderline-ovarian-tumour-desiring-to-preserve-fertility-case-report-and-literature-review-on-the-current-state-of-fertility-preservation-in-women-with-borderline-ovarian-tumours
#16
S Findeklee, L Lotz, K Heusinger, I Hoffmann, R Dittrich, M W Beckmann
Borderline ovarian tumours are semimalignant tumours occurring unilaterally or bilaterally with a peak incidence among women of reproductive age. Since the affected women often wish to preserve fertility, particular precautions must be taken when counselling the patient and obtaining consent prior to planning an individual treatment. Options for preserving fertility include an organ-sparing surgical procedure and cryopreservation of oocytes and/or ovarian tissue. In this article, we report on a 25-year-old patient with a bilateral seromucinous borderline tumour who desired all fertility-preserving options...
November 2016: Geburtshilfe und Frauenheilkunde
https://www.readbyqxmd.com/read/27903287/high-content-imaging-quantification-of-multiple-in-vitro-human-neurogenesis-events-after-neurotoxin-exposure
#17
Xian Wu, Anirban Majumder, Robin Webb, Steven L Stice
BACKGROUND: Our objective was to test neural active compounds in a human developmental neurotoxicity (DNT) model that represents neural tube stages of vulnerability. Previously we showed that 14 days in vitro (DIV 14) was sufficient to generate cryopreserved neuronal cells for post thaw neurite recovery assays. However, short exposure and assessment may not detect toxicants that affect an early neurogenesis continuum, from a mitotic human neural progenitor (hNP) cell population through the course of neurite outgrowth in differentiating neurons...
December 1, 2016: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/27903244/cryopreservation-of-dermal-fibroblasts-and-keratinocytes-in-hydroxyethyl-starch-based-cryoprotectants
#18
Yahaira Naaldijk, Adiv A Johnson, Annett Friedrich-Stöckigt, Alexandra Stolzing
BACKGROUND: Preservation of human skin fibroblasts and keratinocytes is essential for the creation of skin tissue banks. For successful cryopreservation of cells, selection of an appropriate cryoprotectant agent (CPA) is imperative. The aim of this study was to identify CPAs that minimize toxic effects and allow for the preservation of human fibroblasts and keratinocytes in suspension and in monolayers. RESULTS: We cryopreserved human fibroblasts and keratinocytes with different CPAs and compared them to fresh, unfrozen cells...
December 1, 2016: BMC Biotechnology
https://www.readbyqxmd.com/read/27898247/challenges-for-the-maintenance-and-cryopreservation-of-multiple-isolates-of-model-microorganisms-an-example-using-the-marine-diatom-skeletonema-marinoi
#19
John G Day, Simon Tytor, Jenny Egardt, Monica Applegren, Cecilia Rad-Menéndez, Olga Chepurnova, Wim Vyverman, Anna Godhe
Modern genomic and metabolomic tools have provided the possibility of generating and interrogating large datasets that can provide answers to previously imponderable taxonomic, evolutionary, ecological, and physiological questions. However, the curatorial tools needed to provide and maintain the relevant biological resources on which new knowledge can be built have not kept pace with this meteoric rise in scientific capacity, its associated activity, or the huge increase in published science. The availability of biological material of guaranteed identity and quality in Biological Resource Centers is fundamental for scientific research, but it crucially depends on there being adequate preservation/maintenance methods that are capable of ensuring phenotypic, genotypic, and functional security of the biological material(s)...
November 29, 2016: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/27895910/proteomic-identification-of-cryostress-in-epididymal-spermatozoa
#20
Sung Jae Yoon, Md Saidur Rahman, Woo Sung Kwon, Do Yeal Ryu, Yoo Jin Park, Myung Geol Pang
BACKGROUND: Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation, thereby reducing sperm quality. We assessed the motility (%), motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence staining...
2016: Journal of Animal Science and Biotechnology
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