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cell internalization nanoparticles

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https://www.readbyqxmd.com/read/29343454/rational-design-and-evaluation-of-hbsag-polymeric-nanoparticles-as-antigen-delivery-carriers
#1
Hitesh Kumar Dewangan, Tarun Pandey, Lakshmi Maurya, Sanjay Singh
The present work is focused on the development and evaluation of single dose sustained-release Hepatitis B surface antigen (HBsAg) loaded nanovaccine for Hepatitis B. The conventional treatment suffers from repeated administration and hence requires a booster dose. Therefore, polymeric nanovaccine of HBsAg was developed by double emulsion solvent evaporation technique, utilizing central composite design for formulation optimization. The effects of independent variables (like polymer amount, stabilizer concentration, aqueous/organic phase ratio and homogenizer speed) were also studied on critical quality attributes like particle size and entrapment efficiency...
January 14, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29338574/protein-nanoparticles-are-nontoxic-tuneable-cell-stressors
#2
Marianna Teixeira de Pinho Favaro, Laura Sánchez-García, Alejandro Sánchez-Chardi, Mónica Roldán, Ugutz Unzueta, Naroa Serna, Olivia Cano-Garrido, Adriano Rodrigues Azzoni, Neus Ferrer-Miralles, Antonio Villaverde, Esther Vázquez
AIM: Nanoparticle-cell interactions can promote cell toxicity and stimulate particular behavioral patterns, but cell responses to protein nanomaterials have been poorly studied. RESULTS: By repositioning oligomerization domains in a simple, modular self-assembling protein platform, we have generated closely related but distinguishable homomeric nanoparticles. Composed by building blocks with modular domains arranged in different order, they share amino acid composition...
January 17, 2018: Nanomedicine
https://www.readbyqxmd.com/read/29338258/nanoparticle-cell-interactions-surface-chemistry-effects-on-the-cellular-uptake-of-biocompatible-block-copolymer-assemblies
#3
Carlos Eduardo Castro, Caroline Arana da Silva Ribeiro, Alex C Alavarse, Lindomar J C Albuquerque, Maria Cristina Carlan da Silva, Eliézer Jager, František Surman, Vanessa Schmidt, Cristiano Giacomelli, Fernando Carlos Giacomelli
The development of nanovehicles for intracellular drug delivery is strongly bound to the understating and control of nanoparticles cellular uptake process, which in turn is governed by surface chemistry. In this study, we explored the synthesis, characterization and cellular uptake of block copolymer assemblies consisting of a pH-responsive poly[2-(diisopropylamino)ethyl methacrylate] (PDPA) core stabilized by three different biocompatible hydrophilic shells (a zwitterionic type poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) layer, a highly hydrated poly(ethylene oxide) (PEO) layer with stealth effect, and an also proven non-toxic and non-immunogenic poly(N-(2-hydroxypropyl)methacrylamide) (PHPMA) layer)...
January 16, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29336451/the-uptake-retention-and-clearance-of-drug-loaded-dendrimer-nanoparticles-in-astrocytes-electrophysiological-quantification
#4
Helena H Chowdhury, Susana R Cerqueira, Nuno Sousa, Joaquim M Oliveira, Rui L Reis, Robert Zorec
Nanoparticle-based drug delivery systems may impose risks to patients due to potential toxicity associated with a lack of clearance from cells or prolonged carrier-cell retention. This work evaluates vesicular cell uptake, retention and the possible transfer of endocytosed methylprednisolone-loaded carboxymethylchitosan/poly(amidoamine) dendrimer nanoparticles (NPs) into secretory vesicles of rat cultured astrocytes. The cells were incubated with NPs and unitary vesicle fusions/fissions with the plasma membrane were monitored employing high-resolution membrane capacitance measurements...
January 16, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29334216/lysosome-independent-intracellular-drug-gene-co-delivery-by-lipoprotein-derived-nanovector-for-synergistic-apoptosis-inducing-cancer-targeted-therapy
#5
Wei Wang, Kerong Chen, Yujie Su, Jielei Zhang, Min Li, Jianping Zhou
In this paper, reconstituted high-density lipoprotein (rHDL), a lipoprotein-derived nanovector, were constructed for co-delivery of paclitaxel (PTX) and wild type p53 gene (p53). The particle size and the zeta potential of PTX-DODAB/p53-rHDL nanoparticles were 177.2 nm and -20.06 mV, respectively. Meanwhile, they exhibited great serum stability and satisfactory sustained release characteristics in vitro. PTX-DODAB/pDNA-rHDL nanoparticles simultaneously improved the cellular uptake of PTX and pDNA via scavenger receptor B type I (SR-BI) mediated lysosome-independent internalization and promoted the transfection of pDNA in MCF-7 cells, which were revealed by flow cytometry and confocal laser scanning microscopy analyses...
January 15, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29332815/single-particle-analysis-of-tio2-in-candy-products-using-triple-quadrupole-icp-ms
#6
S Candás-Zapico, D J Kutscher, M Montes-Bayón, J Bettmer
Titanium dioxide (TiO2) belongs to the materials that have gained great importance in many applications. In its particulate form (micro- or nanoparticles), it has entered a huge number of consumer products and food-grade TiO2, better known as E171 within the European Union, represents an important food additive. Thus, there is an increasing need for analytical methods able to detect and quantify such particles. In this regard, inductively coupled-mass spectrometry (ICP-MS), in particular single particle ICP-MS (spICP-MS), has gained importance due to its simplicity and ease of use...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29331387/tumor-associated-macrophages-and-angiogenesis-dual-recognizable-nanoparticles-for-enhanced-cancer-chemotherapy
#7
Duo Cao, Lin Liang, Yixin Xu, Ji Sun, Meng Lei, Man Wang, Yahui Wei, Zhenliang Sun
Tumor-associated macrophages (TAMs) and angiogenesis are increasingly considered as the pivotal factors that affect tumor progress. Herein, we developed the paclitaxel (PTX)-loaded nanoparticles (NP/PTX) and decorated it with an innovative peptide YI (YINP/PTX) for simultaneously targeting delivery of drug to TAMs and angiogenesis. We demonstrated that the modification of YI peptide significantly enhanced the internalization of nanoparticles by cells, accumulation of nanoparticles in tumor tissues, but down regulated the distribution of them in normal tissues especially the liver...
January 10, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29330050/production-and-characterization-of-a-novel-site-specific-modifiable-anti-ox40-receptor-single-chain-variable-fragment-for-targeted-drug-delivery
#8
Aki Tanabe, Kazumi Nakano, Makoto Nakakido, Satoru Nagatoishi, Yuetsu Tanaka, Kouhei Tsumoto, Kaoru Uchimaru, Toshiki Watanabe
OX40 receptor (tumor necrosis factor receptor superfamily, member 4; CD134) is a T-cell co-stimulatory molecule that plays an important role in T-cell activation and survival. OX40 receptor is activated by its ligand, OX40L; and modulation of the OX40-OX40L interaction is a promising target for the treatment of autoimmune diseases and cancers. Here, we generated a high-affinity anti-OX40 single-chain variable fragment carrying a C-terminal cysteine residue (scFvC). Physicochemical and functional analyses revealed that the scFvC bound to OX40-expressing cells and was internalized via OX40-mediated endocytosis without inducing phosphorylation of IκBα (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), an important complex in the classical NFκB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathway...
January 9, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29327011/highly-reversible-and-fast-sodium-storage-boosted-by-improved-interfacial-and-surface-charge-transfer-derived-from-the-synergistic-effect-of-heterostructures-and-pseudocapacitance-in-sno2-based-anodes
#9
Xin Li, Xiaohong Sun, Zhiwen Gao, Xudong Hu, Rui Ling, Shu Cai, Chunming Zheng, Wenbin Hu
Sodium-ion batteries have attracted worldwide attention as potential alternatives for large scale stationary energy storage due to the rich reserves and low cost of sodium resources. However, the practical application of sodium-ion batteries is restricted by unsatisfying capacity and poor rate capability. Herein, a novel mechanism of improving both interfacial and surface charge transfer is proposed by fabricating a graphene oxide/SnO2/Co3O4 nanocomposite through a simple hydrothermal method. The formation of heterostructures between ultrafine SnO2 and Co3O4 could enhance the charge transfer of interfaces owing to the internal electric field...
January 12, 2018: Nanoscale
https://www.readbyqxmd.com/read/29321571/thrombin-fe3o4-nanoparticles-for-use-as-a-hemostatic-agent-in-internal-bleeding
#10
Emiliya M Shabanova, Andrey S Drozdov, Anna F Fakhardo, Ivan P Dudanov, Marina S Kovalchuk, Vladimir V Vinogradov
Bleeding remains one of the main causes of premature mortality at present, with internal bleeding being the most dangerous case. In this paper, magnetic hemostatic nanoparticles are shown for the first time to assist in minimally invasive treatment of internal bleeding, implying the introduction directly into the circulatory system followed by localization in the bleeding zone due to the application of an external magnetic field. Nanoparticles were produced by entrapping human thrombin (THR) into a sol-gel derived magnetite matrix followed by grinding to sizes below 200 nm and subsequent colloidization...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29314819/modulating-phagocytic-cell-sequestration-by-tailoring-nanoconstruct-softness
#11
Roberto Palomba, Anna Lisa Palange, Ilaria Francesca Rizzuti, Miguel Ferreira, Antonio Cervadoro, Maria Grazia Barbato, Claudio Canale, Paolo Decuzzi
The effect of nanoparticle size, shape and surface properties on cellular uptake has been extensively investigated for its basic science and translational implications. Recently, softness is emerging as a design parameter for modulating the interaction of nanoparticles with cells and the biological microenvironment. Here, circular, quadrangular and elliptical polymeric nanoconstructs of different sizes are realized with a Young's modulus ranging from ∽ 100 kPa (soft) to 10 MPa (rigid). The interaction of these nanoconstructs with professional phagocytic cells is assessed via confocal microscopy and flow cytometry analyses...
January 9, 2018: ACS Nano
https://www.readbyqxmd.com/read/29313384/chitosan-nanoparticles-triggered-the-induction-of-ros-mediated-cytoprotective-autophagy-in-cancer-cells
#12
Hao Wang, Xiwei Yu, Chang Su, Yijie Shi, Liang Zhao
There is a close relationship between autophagy and apoptosis during cancer cell death. We used chitosan nanoparticles (CS NPs) to explore the effects of internalized NPs on the induction of autophagy and to confirm the role of autophagic responses elicited by nanomaterials on the tumour cell's fate. CS NPs at nontoxic concentrations ranging from 10-100 μg/mL triggered the induction of autophagy. With the addition of CS NPs, the aggregation of endogenous LC3 was significantly enhanced and acidic autophagic bodies had been accumulated...
January 9, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29313073/correction-to-characterization-of-the-first-fully-human-anti-tem1-scfv-in-models-of-solid-tumor-imaging-and-immunotoxin-based-therapy
#13
Xiaopeng Yuan, Mingjuan Yang, Xiang Chen, Xuhua Zhang, Shrey Sukhadia, Najia Musolino, Huijing Bao, Tingtao Chen, Chen Xu, Qirui Wang, Stephen Santoro, Daniel Ricklin, Jia Hu, Ruihe Lin, Wei Yang, Zhijun Li, Weijun Qin, Aizhi Zhao, Nathalie Scholler, George Coukos
Tumor endothelial marker 1 (TEM1) has been identified as a novel surface marker upregulated on the blood vessels and stroma in many solid tumors. We previously isolated a novel single-chain variable fragment (scFv) 78 against TEM1 from a yeast display scFv library. Here we evaluated the potential applications of scFv78 as a tool for tumor molecular imaging, immunotoxin-based therapy and nanotherapy. Epitope mapping, three-dimensional (3D) structure docking and affinity measurements indicated that scFv78 could bind to both human and murine TEM1, with equivalent affinity, at a well-conserved conformational epitope...
January 8, 2018: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/29306846/d-l-lysine-functionalized-fe3o4-nanoparticles-for-detection-of-cancer-cells
#14
Iryna Antal, Martina Koneracka, Martina Kubovcikova, Vlasta Zavisova, Iryna Khmara, Dasa Lucanska, Lenka Jelenska, Ivana Vidlickova, Miriam Zatovicova, Silvia Pastorekova, Nikola Bugarova, Matej Micusik, Maria Omastova, Peter Kopcansky
Amino-modified magnetic nanoparticles were prepared by direct chemisorption of biocompatible d,l-lysine (DLL) on electrostatically stabilized magnetic nanoparticles with the aim to bind specific antibodies (Ab) able to detect cancer cells. The magnetic nanoparticles prepared by coprecipitation were stabilized in an acidic medium. A full optimization study of amino modification performed by UV/Vis spectroscopy and Dynamic Light Scattering measurement (DLS) confirmed an optimal DLL/Fe3O4 weight ratio of 2. The sample was subjected to complex characterizations using different techniques such as UV/Vis, FTIR and X-ray photoelectron spectroscopies (XPS) together with transmission electron microscopy and size/zeta potential measurements...
December 21, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29299922/stabilization-of-human-tyrosine-hydroxylase-in-maltodextrin-nanoparticles-for-delivery-to-neuronal-cells-and-tissue
#15
Maria Teresa Bezem, Fredrik Gullaksen Johannessen, Kunwar Jung-Kc, Edvin Tang Gundersen, Ana Jorge-Finnigan, Ming Ying, Didier Betbeder, Lars Herfindal, Aurora Martinez
Enzyme replacement therapy (ERT) is a therapeutic approach envisioned decades ago for the correction of genetic disorders, but it is only after improvements in recombinant protein expression and purification that the applicability of this approach has increased. Customarily, ERT has been less successful for the correction of disorders with neurological manifestations. In this work we have tested the functionality of nanoparticles (NP) composed of maltodextrin with a lipid core to bind and stabilize tyrosine hydroxylase (TH), which is a complex and unstable brain enzyme that catalyzes the rate limiting step in the synthesis of dopamine and other catecholamine neurotransmitters...
January 4, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29296980/hybrid-fept-sio2-au-nanoparticles-as-a-theranostic-tool-in-vitro-photo-thermal-treatment-and-mri-imaging
#16
N Kostevšek, I Abramovič, S Hudoklin, M E Kreft, I Serša, A Sepe, J Vidmar, S Šturm, M Spreitzer, J Ščančar, S Kobe, K Žužek Rožman
We have produced an innovative, theranostic material based on FePt/SiO2/Au hybrid nanoparticles (NPs) for both, photo-thermal therapy and magnetic resonance imaging (MRI). Furthermore, a new synthesis approach, i.e., Au double seeding, for the preparation of Au nanoshells around the FePt/SiO2 cores, is proposed. The photo-thermal and the MRI response were first demonstrated on an aqueous suspension of hybrid FePt/SiO2/Au NPs. The cytotoxicity together with the internalization mechanism and the intracellular fate of the hybrid NPs were evaluated in vitro on a normal (NPU) and a half-differentiated cancerous cell line (RT4)...
January 3, 2018: Nanoscale
https://www.readbyqxmd.com/read/29291502/polyglycerol-grafting-and-rgd-peptide-conjugation-on-mno-nanoclusters-for-enhanced-colloidal-stability-selective-cellular-uptake-and-cytotoxicity
#17
Xiaoxin Yang, Li Zhao, Luyi Zheng, Meiyun Xu, Xiulan Cai
An efficient surface engineering strategy for MnO nanoparticles was developed to attain enhanced colloidal stability, selective uptake by and toxicity to specific cancer cells. Specifically, MnO nanoclusters prepared by polyol method were grafted with polyglycerol (MnO-PG), and then conjugated with arginine-glycine-aspartate peptide (MnO-PG-RGD) through stepwise organic reactions. The physicochemical properties of the surface engineered MnO nanoclusters were characterized by powder X-ray diffraction, Fourier transform infrared spectroscopy, thermogravimetric analysis, dynamic light scattering, zeta potential, transmission electron microscopy and high-resolution transmission electron microscopy...
December 20, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29291159/dual-radiosensitization-and-anti-stat3-anti-proliferative-strategy-based-on-delivery-of-gold-nanoparticle-oligonucleotide-nanoconstructs-to-head-and-neck-cancer-cells
#18
Surong Zhang, Suresh Gupta, Thomas J Fitzgerald, Alexei A Bogdanov
Constitutively activated signal transducer and activator of transcription 3 (STAT3) factor is an important therapeutic target in head and neck cancer (HNC). Despite early promising results, a reliable systemic delivery system for STAT3- targeted oligonucleotide (ODN) drugs is still needed for future clinical translation of anti-STAT3 therapies. We engineered and tested a novel ODN duplex/gold nanoparticle (AuNP)-based system carrying a therapeutic STAT3 decoy (STAT3d) payload. This strategy is two-pronged because of the additive STAT3 antagonism and radiosensitizing properties of AuNP...
2018: Nanotheranostics
https://www.readbyqxmd.com/read/29290284/oral-cancer-derived-exosomal-nap1-enhances-cytotoxicity-of-natural-killer-cells-via-the-irf-3-pathway
#19
Yingnan Wang, Xing Qin, Xueqin Zhu, Wanjun Chen, Jianjun Zhang, Wantao Chen
OBJECTIVE: To examine the effects of oral cancer-derived exosomes (OCEXs) on natural killer (NK) cells and to explore the underlying mechanism. MATERIALS AND METHODS: OCEXs were isolated from the cell culture supernatant of oral cancer (OC) cells using ultrafiltration and affinity chromatography and were identified using electron microscopy, nanoparticle tracking analysis (NTA) and immunoblotting. The effects of OCEXs on NK cells were analyzed using laser scanning confocal microscopy and several functional assays of NK cells...
January 2018: Oral Oncology
https://www.readbyqxmd.com/read/29288282/promotion-of-sh-sy5y-cell-growth-by-gold-nanoparticles-modified-with-6-mercaptopurine-and-a-neuron-penetrating-peptide
#20
Yaruo Xiao, Enqi Zhang, Ailing Fu
Much effort has been devoted to the discovery of effective biomaterials for nerve regeneration. Here, we reported a novel application of gold nanoparticles (AuNPs) modified with 6-mercaptopurine (6MP) and a neuron-penetrating peptide (RDP) as a neurophic agent to promote proliferation and neurite growth of human neuroblastoma (SH-SY5Y) cells. When the cells were treated with 6MP-AuNPs-RDP conjugates, they showed higher metabolic activity than the control. Moreover, SH-SY5Y cells were transplanted onto the surface coated with 6MP-AuNPs-RDP to examine the effect of neurite development...
December 29, 2017: Nanoscale Research Letters
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