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https://www.readbyqxmd.com/read/28641542/functional-nanoparticles-and-their-interactions-with-mesenchymal-stem-cells
#1
Weiwei Wang, Zijun Deng, Xun Xu, Zhengdong Li, Friedrich Jung, Nan Ma, Andreas Lendlein
Mesenchymal stem cells (MSCs) have become one of the most important cell sources for regenerative medicine. However, some mechanisms of MSC-based therapy are still not fully understood. The clinical outcome may be restricted by some MSC-related obstacles such as the low survival rate, differentiation into undesired lineages and malignant transformation. In recent years, with the emergence of nanotechnology, various types of multifunctional nanoparticles (NPs) have been designed, prepared and explored for bio-related applications...
June 22, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28640995/colloidal-gold-nanoparticles-induce-changes-in-cellular-and-subcellular-morphology
#2
Xiaowei Ma, Raimo Hartmann, Dorleta Jimenez de Aberasturi, Fang Yang, Stefaan J H Soenen, Bella B Manshian, Jonas Franz, Daniel Valdeperez, Beatriz Pelaz, Neus Feliu, Norbert Hampp, Christoph Riethmüller, Henning Vieker, Natalie Frese, Armin Gölzhäuser, Michael Simonich, Robert L Tanguay, Xing-Jie Liang, Wolfgang J Parak
Exposure of cells to colloidal nanoparticles (NPs) can have concentration-dependent harmful effects. Mostly, such effects are monitored with biochemical assays or probes from molecular biology, i.e. viability assays, gene expression profiles, etc., neglecting that the presence of NPs can also drastically affect cellular morphology. In the case of polymer-coated Au NPs we demonstrate that upon NP internalization, cells undergo lysosomal swelling, alterations in mitochondrial morphology, disturbances in actin and tubulin cytoskeleton and associated signaling, and reduction of focal adhesion contact area and number of filopodia...
June 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/28640590/chlorin-e6-functionalized-theranostic-multistage-nanovectors-transported-by-stem-cells-for-effective-photodynamic-therapy
#3
Simo Näkki, Jonathan Martinez, Michael Evangelopoulos, Wujun Xu, Vesa-Pekka Lehto, Ennio Tasciotti
Approaches to achieve site-specific and targeted delivery that provide an effective solution to reduce negative, off target side-effects are urgently needed for cancer therapy. Here, we utilized a Trojan horse-like strategy to carry photosensitizer Chlorin e6 conjugated porous silicon multistage nanovectors with tumor homing mesenchymal stem cells for targeted photodynamic therapy and diagnosis. The inherent versatility of multistage nanovectors permitted the conjugation of photosensitizers to enable precise cell death induction (60%) upon photodynamic therapy, while simultaneously retaining the loading capacity to load various payloads, such as antitumor drugs and diagnostic nanoparticles...
June 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28636315/fabrication-of-hierarchical-macroporous-biocompatible-scaffolds-by-combining-pickering-high-internal-phase-emulsion-templates-with-three-dimensional-printing
#4
Ting Yang, Yang Hu, Chaoyang Wang, Bernard P Binks
Biocompatible and biodegradable porous scaffolds with adjustable pore structure have aroused increasing interest in bone tissue engineering. Here, we report a facile method to fabricate hierarchical macroporous biocompatible (HmPB) scaffolds by combining Pickering high internal phase emulsion (HIPE) templates with three-dimensional (3D) printing. HmPB scaffolds composed of a polymer matrix of poly(L-lactic acid), PLLA, and poly(ε-caprolactone), PCL, are readily fabricated by solvent evaporation of 3D printed Pickering HIPEs which are stabilized by hydrophobically modified silica nanoparticles (h-SiO2)...
June 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28632911/polymer-functionalized-gold-nanoparticles-as-non-viral-gene-delivery-reagents
#5
M Mar Encabo-Berzosa, Maria Sancho-Albero, Victor Sebastian, Silvia Irusta, Manuel Arruebo, Jesus Santamaria, Pilar Martín Duque
BACKGROUND: In this study we investigated the ability of PEG functionalized gold nanoparticles as non-viral vectors in the transfection of different cell lines, comparing them with commercial lipoplexes. METHODS: Positively charged gold nanoparticles were synthesized using PEI as reducing and stabilizer agent and its cytotoxicity reduced by its functionalization with PEG. We bound the nanoparticles to three plasmids with different sizes (4-40 kpb). The vector internalization was evaluated by confocal and electronic microscopy...
June 20, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28630477/the-effect-of-shape-on-cellular-uptake-of-gold-nanoparticles-in-the-forms-of-stars-rods-and-triangles
#6
Xueping Xie, Jinfeng Liao, Xiaoru Shao, Qianshun Li, Yunfeng Lin
Gold nanomaterials have attracted considerable interest as vehicles for intracellular drug delivery. In our study, we synthesized three different shapes of methylpolyethylene glycol coated-anisotropic gold nanoparticles: stars, rods, and triangles. The cellular internalization of these nanoparticles by RAW264.7 cells was analyzed, providing a parametric evaluation of the effect of shape. The efficiency of cellular uptake of the gold nanoparticles was found to rank in the following order from lowest to highest: stars, rods, and triangles...
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28626876/optical-molecular-imaging-guided-radiation-therapy-part-2-integrated-x-ray-and-fluorescence-molecular-tomography
#7
Junwei Shi, Thirupandiyur S Udayakumar, Zhiqun Wang, Nesrin Dogan, Alan Pollack, Yidong Yang
PURPOSE: Differentiating tumor from its surrounding soft tissues is challenging for x-ray computed tomography (CT). Fluorescence molecular tomography (FMT) can directly localize the internal tumors targeted with specific fluorescent probes. A FMT system was developed and integrated onto a CT guided irradiator to improve tumor localization for image guided radiation. METHODS: The FMT system was aligned orthogonal to the cone beam CT onboard our previously developed image guided small animal arc radiation treatment system (iSMAART)...
June 19, 2017: Medical Physics
https://www.readbyqxmd.com/read/28624659/surface-engineering-of-solid-lipid-nanoparticle-assemblies-by-methyl-%C3%AE-d-mannopyranoside-for-the-active-targeting-to-macrophages-in-anti-tuberculosis-inhalation-therapy
#8
Eleonora Maretti, Luca Costantino, Cecilia Rustichelli, Eliana Leo, Maria Antonietta Croce, Francesca Buttini, Eleonora Truzzi, Valentina Iannuccelli
This study describes the development of new mannosylated Solid Lipid Nanoparticle assemblies (SLNas) delivering rifampicin for an inhaled treatment of tuberculosis. SLNas were surface engineered with mannose residues to recognize mannose receptors located on infected alveolar macrophages and facilitate cell internalization. Two sets of SLNas were produced by the melt emulsifying technique using biocompatible lipid components, i.e. cholesteryl myristate combined with palmitic acid (PA set) or tripalmitin (TP set), in the presence of the targeting moiety, methyl α-d-mannopyranoside...
June 15, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28624185/the-formation-of-nanoparticles-between-small-interfering-rna-and-amphipathic-cell-penetrating-peptides
#9
Ly Pärnaste, Piret Arukuusk, Kent Langel, Tanel Tenson, Ülo Langel
Cell-penetrating peptides (CPPs) are delivery vectors widely used to aid the transport of biologically active cargoes to intracellular targets. These cargoes include small interfering RNAs (siRNA) that are not naturally internalized by cells. Elucidating the complexities behind the formation of CPP and cargo complexes is crucial for understanding the processes related to their delivery. In this study, we used modified analogs of the CPP transportan10 and investigated the binding properties of these CPPs to siRNA, the formation parameters of the CPP/siRNA complexes, and their stabiliy to enzymatic degradation...
June 16, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28623338/high-resolution-imaging-of-a-cell-attached-nanointerface-using-a-gold-nanoparticle-two-dimensional-sheet
#10
Shihomi Masuda, Yuhki Yanase, Eiji Usukura, Sou Ryuzaki, Pangpang Wang, Koichi Okamoto, Thasaneeya Kuboki, Satoru Kidoaki, Kaoru Tamada
This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28619643/superior-transfection-efficiency-of-phagocytic-astrocytes-by-large-chitosan-dna-nanoparticles
#11
Fanqiang Kong, Guoping Liu, Shumin Zhou, Jie Guo, Song Chen, Zhiyun Wang
PURPOSE: Mechanism study of why astrocytes isolated from experimental autoimmune encephalomyelitis (EAE)-induced B6 mice or after being exposed to inflammatory factors had the highest transfection efficiency to larger-sized, but not compacted, pspCS/pDNA particles. METHODS: Phosphorylatable short peptide conjugated chitosan (pspCS) was compounded with plasmid DNA (pDNA) at different N:P ratios to form pspCS/pDNA particles of different size and zeta potentials. These pspCS/pDNA particles were used for the transfection of astrocytes isolated from either EAE induced or healthy B6 mice...
June 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28618976/bromelain-decorated-hybrid-nanoparticles-based-on-lactobionic-acid-conjugated-chitosan-for-in%C3%A2-vitro-anti-tumor-study
#12
Bing Wei, Le He, Xin Wang, Guo Qing Yan, Jun Wang, Rupei Tang
In this work, lactobionic acid-modified chitosan (CLA) was chosen as an initial material to prepare tumor-targeted nanoparticles (CLA NPs). To improve the nanoparticles' tumor penetration ability, bromelain was then decorated on the surface of CLA NPs to give CLAB NPs. The micromorphology of CLA and CLAB NPs was observed by transmission electron microscopy and scanning electron microscopy. The stability of CLA and CLAB NPs was then investigated at different pH values (4.0-9.0) and physiological environment by dynamic light scattering...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28617063/matrix-metalloproteases-responsive-nanomaterials-for-tumor-targeting-diagnosis-and-treatment
#13
Jingyuan Xiong, Huile Gao
Tumor poses tremendous challenges to mankind. In various tumors, matrix metalloproteases (MMPs) are ubiquitously over expressed and participate throughout the process of tumor development. MMPs are commonly used as internal stimuli, and MMPs responsive nanomaterials are classified into three categories according to the drug delivery process: administration and distribution to tumor, intratumoral distribution and cell internalization, and specific drug release. Firstly, MMPs sensitive polyethylene glycol (PEG) de-shielding and activatable cell penetrating peptides were developed to improve the blood circulation time of drug delivery systems, thereby, enhancing tumor or stroma cells recognition and penetration...
June 15, 2017: Journal of Microencapsulation
https://www.readbyqxmd.com/read/28610486/resveratrol-loaded-glycyrrhizic-acid-conjugated-human-serum-albumin-nanoparticles-wrapping-resveratrol-nanoparticles-preparation-characterization-and-targeting-effect-on-liver-tumors
#14
Mingfang Wu, Bolin Lian, Yiping Deng, Ziqi Feng, Chen Zhong, Weiwei Wu, Yannian Huang, Lingling Wang, Chang Zu, Xiuhua Zhao
In this study, glycyrrhizic acid-conjugated human serum albumin nanoparticles wrapping resveratrol nanoparticles were prepared to establish a tumor targeting nano-sized drug delivery system. Glycyrrhizic acid was coupled to human serum albumin, and resveratrol was encapsulated in glycyrrhizic acid-conjugated human serum albumin by high-pressure homogenization emulsification. The average particle size of sample nanoparticles prepared under the optimal conditions was 108.1 ± 5.3 nm with a polydispersity index (PDI) of 0...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28608695/molecular-elucidation-of-biological-response-to-mesoporous-silica-nanoparticles-in-vitro-and-in-vivo
#15
Cheng-Chung Chou, Wei Chen, Yann Hung, Chung-Yuan Mou
Biomedical applications of mesoporous silica nanoparticles (MSNs) require efficient cellular uptake and low toxicity. The purpose of this study is to investigate the cellular uptake and toxicity of MSNs with different sizes and charges (50, 100 and 250 nm with a positive surface charge and 100 nm with a negative surface charge) exposed to human monocyte-derived macrophages, lung epithelium BEAS-2B cells and mice using genome-wide gene expression analysis and cellular/animal-level end point tests. We found that MSNs can be taken up into cells through endocytosis in a charge- and size-dependent manner, with positively charged and larger MSNs being more easily taken up into the cells by recruiting more types of endocytotic pathways for more cellular uptake...
June 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28608677/block-copolymer-based-nanoparticles-for-theranostic-intervention-of-cervical-cancer-synthesis-pharmacokinetics-and-in-vitro-in-vivo-evaluation-in-hela-xenograft-models
#16
Shweta Dumoga, Yogesh Rai, Anant Narayan Bhatt, Anjani Kumar Tiwari, Surendra Singh, Anil Kumar Mishra, Dipti Kakkar
Polymer based nanoparticles have proven to be viable carriers of therapeutic agents. In this study we have developed nanoparticles (NPs) from polypeptide-polyethylene glycol based triblock and diblock copolymers. The synthesized block copolymers Poly (ethylene glycol)-b-Poly (glutamic acid)-b- Poly (ethylene glycol) (GEG) and Poly (ethylene glycol)-b-Poly (glutamic acid) (EG) conjugated with folic acid for targeting specificity (EGFA), have been used to encapsulate methotrexate (MTX) to form M-GEG and M-EGFA nanoparticles (NPs) aimed at passive and active targeting of cervical carcinoma...
June 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28605176/reduction-and-ph-sensitive-hyaluronan-nanoparticles-for-delivery-of-iridium-iii-anticancer-drugs
#17
Zhixiang Cai, Hongbin Zhang, Yue Wei, Yuanyuan Wei, Yanping Xie, Fengsong Cong
The organoiridium(III) complex (Ir(III)) [(η(5)-Cp(xbiph))Ir(phpy) (py)]PF6 containing π-bonded biphenyltetramethylcyclopentadienyl(Cp(xbiph)), C∧N-chelated phenylpyridine(phpy), and pyridine (py) ligands has more potent antitumor activity as a new generation of drug than cisplatin toward various cancer cells. However, poor site-specific delivery, low solubility, and poor tumor penetration are common limitations of chemotherapy drugs. To develop CD44-targetable, pH-, and reduction-responsive drug delivery systems for Ir(III) drugs, the amphiphilic hyaluronan (HA)-based conjugates of HA-cystamin-pyrenyl (HA-ss-Py) containing disulfide bonds and HA-pyrenyl (HA-Py) were designed...
June 22, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28604134/impact-of-exposure-time-particle-size-and-uptake-pathway-on-silver-nanoparticle-effects-on-circulating-immune-cells-in-mytilus-galloprovincialis
#18
Younes Bouallegui, Ridha Ben Younes, Faten Turki, Ridha Oueslati
Nanomaterials have increasingly emerged as potential pollutants to aquatic organisms. Nanomaterials are known to be taken up by hemocytes of marine invertebrates including Mytilus galloprovincialis. Indeed, assessments of hemocyte-related parameters are a valuable tool in the determination of potentials for nanoparticle (NP) toxicity. The present study assessed the effects from two size types of silver nanoparticles (AgNP: <50 nm and <100 nm) on the frequency of hemocytes subpopulations as immunomodulation biomarkers exposed in a mollusk host...
December 2017: Journal of Immunotoxicology
https://www.readbyqxmd.com/read/28597662/chitosan-hyaluronic-acid-nanoparticles-rational-design-revisited-for-rna-delivery
#19
Enrique Lallana, Julio M Rios de la Rosa, Annalisa Tirella, Maria Pelliccia, Arianna Gennari, Ian J Stratford, Sanyogitta Puri, Marianne Ashford, Nicola Tirelli
Chitosan/hyaluronic acid (HA) nanoparticles can be used to deliver an RNA/DNA cargo to cells overexpressing HA receptors such as CD44. For these systems, unequivocal links have not been established yet between chitosan macromolecular (molecular weight; degree of deacetylation, i.e., charge density) and nanoparticle variables (complexation strength, i.e., stability; nucleic acid protection; internalization rate) on one hand, and transfection efficiency on the other hand. Here, we have focused on the role of avidity on transfection efficiency in the CD44-expressing HCT-116 as a cellular model; we have employed two differently sized payloads (a large luciferase-encoding mRNA and a much smaller anti-Luc siRNA), and a small library of chitosans (variable molecular weight and degree of deactylation)...
June 19, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28595381/enhanced-internalization-of-macromolecular-drugs-into-mycobacteriumsmegmatis-with-the-assistance-of-silver-nanoparticles
#20
Fangfang Sun, Sangjin Oh, Jeonghyo Kim, Tatsuya Kato, Hwa-Jung Kim, Jaebeom Lee, Enoch Y Park
In this study, silver nanoparticles (AgNPs) were synthesized by the citrate reduction process and, with the assistance of n-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, were successfully loaded with the macromolecular drug vancomycin (VAM) to form AgNP-VAM bioconjugates. The synthesized AgNPs, VAM, and AgNP-VAM conjugate were characterized by UV-visible spectroscopy, zeta potential analysis, confocal microscopy, and transmission electron microscopy. The effect of loading VAM onto AgNPs was investigated by testing the internalization of the bioconjugate into Mycobacterium smegmatis...
June 8, 2017: Journal of Microbiology and Biotechnology
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