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cell internalization nanoparticles

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https://www.readbyqxmd.com/read/28212584/hyaluronic-acid-serum-albumin-conjugate-based-nanoparticles-for-targeted-cancer-therapy
#1
Ravit Edelman, Yehuda G Assaraf, Inna Levitzky, Tal Shahar, Yoav D Livney
Multiple carcinomas including breast, ovarian, colon, lung and stomach cancer, overexpress the hyaluronic acid (HA) receptor, CD44. Overexpression of CD44 contributes to key cancer processes including tumor invasion, metastasis, recurrence, and chemoresistance. Herein, we devised novel targeted nanoparticles (NPs) for delivery of anticancer chemotherapeutics, comprised of self-assembling Maillard reaction-based conjugates of HA and bovine serum albumin (BSA). HA served as the hydrophilic block, and as the ligand for actively targeting cancer cells overexpressing CD44...
February 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28207276/hydrophobic-nanoparticles-reduce-the-%C3%AE-sheet-content-of-sevi-amyloid-fibrils-and-inhibit-sevi-enhanced-hiv-infectivity
#2
Daniel A Sheik, Jeffrey M Chamberlain, Lauren R Brooks, Melissa L Clark, Young Hun Kim, Geoffray Leriche, Clifford P Kubiak, Stephen Dewhurst, Jerry Yang
Semen-Derived Enhancer of Virus Infection (SEVI) fibrils are naturally abundant amyloid aggregates found in semen that facilitate viral attachment and internalization of HIV in cells, thereby increasing the probability of infection. Mature SEVI fibrils are comprised of aggregated peptides exhibiting high β-sheet secondary structural characteristics. Herein, we show that polymers containing hydrophobic side chains can interact with SEVI and reduce its β-sheet content by ~45% compared to the β-sheet content of SEVI in the presence of polymers with hydrophilic side chains, as estimated by Polarization Modulation-Infrared Reflectance Absorption Spectroscopy Measurements...
February 16, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28203075/co-delivery-of-paclitaxel-and-tos-cisplatin-via-tat-targeted-solid-lipid-nanoparticles-with-synergistic-antitumor-activity-against-cervical-cancer
#3
Bo Liu, Li Han, Junyan Liu, Shumei Han, Zhen Chen, Lixi Jiang
BACKGROUND: Cervical cancer is a major world health problem for women. Currently, cancer research focuses on improving therapy for cervical cancer using various treatment options such as co-delivery of chemotherapeutic agents by nanocarriers. PURPOSE: The aim of this study was to develop trans-activating transcriptional activator (TAT)-modified solid lipid nanoparticles (SLNs) for co-delivery of paclitaxel (PTX) and α-tocopherol succinate-cisplatin prodrug (TOS-CDDP) (TAT PTX/TOS-CDDP SLNs) in order to achieve synergistic antitumor activity against cervical cancer...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28199112/mitochondrial-gene-therapy-advances-in-mitochondrial-gene-cloning-plasmid-production-and-nanosystems-targeted-to-mitochondria
#4
Eduarda Coutinho, Cátia Batista, Fani Sousa, João Queiroz, Diana Costa
Mitochondrial gene therapy seems to be a valuable and promising strategy to treat mitochondrial disorders. The use of a therapeutic vector based on mitochondrial DNA, along with its affinity to the site of mitochondria can be considered a powerful tool in the reestablishment of normal mitochondrial function. In line with this and for the first time, we successfully cloned the mitochondrial gene ND1 that was stably maintained in multi-copy pCAG-GFP plasmid, used to transform E. coli. This mitochondrial gene based plasmid was encapsulated into nanoparticles...
February 15, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28196456/toxicity-of-internalized-laser-generated-pure-silver-nanoparticles-to-the-isolated-rat-hippocampus-cells
#5
Canan Kursungoz, Sadık Taşkın Taş, Mustafa F Sargon, Yıldırım Sara, Bülend Ortaç
Silver nanoparticles (AgNPs) are the most commonly used nanoparticles (NPs) in medicine, industry and cosmetics. They are generally considered as biocompatible. However, contradictory reports on their biosafety render them difficult to accept as 'safe'. In this study, we evaluated the neurotoxicity of direct AgNP treatment in rat hippocampal slices. We produced pure uncoated AgNPs by a pulsed laser ablation method. NP characterization was performed by Ultraviolet (UV) visible spectrophotometer, scanning electron microscope, transmission electron microscope (TEM) and energy-dispersive X-ray spectroscopy...
January 1, 2017: Toxicology and Industrial Health
https://www.readbyqxmd.com/read/28196309/the-impact-of-protein-corona-formation-on-the-macrophage-cellular-uptake-and-biodistribution-of-spherical-nucleic-acids
#6
Alyssa B Chinen, Chenxia M Guan, Caroline H Ko, Chad A Mirkin
The effect of serum protein adsorption on the biological fate of Spherical Nucleic Acids (SNAs) is investigated. Through a proteomic analysis, it is shown that G-quadruplexes templated on the surface of a gold nanoparticle in the form of SNAs mediate the formation of a protein corona that is rich in complement proteins relative to SNAs composed of poly-thymine (poly-T) DNA. Cellular uptake studies show that complement receptors on macrophage cells recognize the SNA protein corona, facilitating their internalization, and causing G-rich SNAs to accumulate in the liver and spleen more than poly-T SNAs in vivo...
February 14, 2017: Small
https://www.readbyqxmd.com/read/28192902/concentration-normalized-electroanalytical-assaying-of-exosomal-markers
#7
Qian Li, George K Tofaris, Jason J Davis
Exosomes are both active in mediating intracellular communication and potentially present a potent cargo of disease biomarkers to an assay. The robust evaluation of exosomal markers could lead to a paradigm shift in clinical analysis and associated care. To date, much of this has been hindered by issues of sample preparation and assay signal-to-noise. We introduce here the use of ultrasensitive electrochemical impedance spectroscopy to quantify both external (tetraspanin) and internal (syntenin) exosome-specific markers...
February 10, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28191966/self-assembled-lyotropic-liquid-crystalline-phase-behaviour-of-monoolein-capric-acid-phospholipid-nanoparticulate-systems
#8
Jiali Zhai, Nhiem Tran, Sampa Sarkar, Celesta Fong, Xavier Mulet, Calum J Drummond
We report here the lyotropic liquid crystalline phase behaviour of two lipid nanoparticulate systems containing mixtures of monoolein, capric acid and saturated diacyl phosphatidylcholines dispersed by the Pluronic F127 block copolymer. Synchrotron small angle X-ray scattering (SAXS) was used to screen the phase behaviour of a library of lipid nanoparticles in a high-throughput manner. It was found that adding capric acid and phosphatidylcholines had opposing effects on the spontaneous membrane curvature of the monoolein lipid layer and hence the internal mesophase of the final nanoparticles...
February 13, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28188686/toxic-effects-of-tio2-nanoparticles-in-primary-cultured-rat-sertoli-cells-are-mediated-via-a-dysregulated-ca-2-pkc-p38-mapk-nf-%C3%AE%C2%BAb-cascade
#9
Lingqun Ye, Fashui Hong, Xiao Ze, Lingjuan Li, Yaoming Zhou, Yuguan Ze
Although numerous studies have demonstrated that titanium dioxide nanoparticles (TiO2 NPs) can be accumulated in various animal organs and can cause toxicity, there is currently only limited data regarding reproductive toxicity especially on the toxic mechanisms of TiO2 NPs in Sertoli cells. In order to investigate the mechanism of reproductive toxicity, primary cultured rat Sertoli cells were exposed to 5, 15 or 30 μg/ml TiO2 NPs for 24 h, and TiO2 NPs internalization, expression of PKC (p-PKC) and p38 MAPK (p-p38 MAPK) as well as calcium homeostasis were examined...
February 11, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28186438/a-semiautomated-microfluidic-platform-for-real-time-investigation-of-nanoparticles-cellular-uptake-and-cancer-cells-tracking
#10
Mariana R Carvalho, F Raquel Maia, Joana Silva-Correia, Bruno M Costa, Rui L Reis, Joaquim M Oliveira
AIM: Develop a platform composed of labeled dendrimer nanoparticles (NPs) and a microfluidic device for real-time monitoring of cancer cells fate. MATERIALS & METHODS: Carboxymethylchitosan/poly(amidoamine) dendrimer NPs were labeled with fluorescein-5(6)-isothiocyanate and characterized using different physicochemical techniques. After, HeLa, HCT-116 and U87MG were cultured in the presence of NPs, and cell viability and internalization efficiency in static (standard culture) and dynamic (microfluidic culture) conditions were investigated...
February 10, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28183597/preparation-and-characterization-of-dox-loaded-keratin-nanoparticles-for-ph-gsh-dual-responsive-release
#11
Yanmei Li, Xuelian Zhi, Jiantao Lin, Xin You, Jiang Yuan
Smart drug carriers are the current need of the hour in controlled drug delivery applications. In this work, pH and redox dual responsive keratin based drug-loaded nanoparticles (KDNPs) were fabricated through two-step strategies. Keratin nanoparticles were first prepared by desolvation method and chemical crosslinking, followed by electrostatic adsorbing doxorubicin (DOX) to afford drug loaded keratin nanoparticles (KDNPs). The size, size distribution, and morphology of the KDNPs were characterized with dynamic light scattering (DLS) and Scan electronic microscope (SEM)...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28182823/how-to-direct-the-neuronal-growth-process-in-peripheral-nerve-regeneration-future-strategies-for-nanosurfaces-scaffold-and-magnetic-nanoparticles
#12
Andrea Poggetti, Pietro Battistini, Paolo Domenico Parchi, Michela Novelli, Simona Raffa, Marco Cecchini, Anna Maria Nucci, Michele Lisanti
Currently, the gold standard to repair large nerve defects is the autologous nerve graft. These solutions offer a mechanical support, adhesion substrates, and, with Schwann cells (SC), a source of neurotropic factors for axonal growth. The technical limits are the donor side damage, multiple surgical accesses, and the unavailability of large amounts of grafts. In recent years, several researchers focused their attention on the interaction between cells (nervous and glial) and physic-chemical cues that arise from the extracellular milieu...
February 7, 2017: Surgical Technology International
https://www.readbyqxmd.com/read/28182147/silica-nanoparticles-induce-autophagy-dysfunction-via-lysosomal-impairment-and-inhibition-of-autophagosome-degradation-in-hepatocytes
#13
Ji Wang, Yongbo Yu, Ke Lu, Man Yang, Yang Li, Xianqing Zhou, Zhiwei Sun
Autophagy dysfunction is considered as a potential toxic mechanism of nanomaterials. Silica nanoparticles (SiNPs) can induce autophagy, but the specific mechanism involved remains unclear. Therefore, the aim of this study was to confirm the effects of SiNPs on autophagy dysfunction and explore the possible underlying mechanism. In this article, we reported that cell-internalized SiNPs exhibited dose- and time-dependent cytotoxicity in both L-02 and HepG2 cells. Multiple methods verified that SiNPs induced autophagy even at the noncytotoxic level and blocked the autophagic flux at the high-dose level...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28166836/intracellular-ph-responsive-and-rituximab-conjugated-mesoporous-silica-nanoparticles-for-targeted-drug-delivery-to-lymphoma-b-cells
#14
Shoubing Zhou, Dan Wu, Xiaodong Yin, Xiaoxiao Jin, Xiu Zhang, Shiya Zheng, Cailian Wang, Yanwen Liu
BACKGROUND: One of the main problems in B cell lymphoma treatment is severe adverse effects and low therapeutic efficacy resulting from systemic chemotherapy. A pH-sensitive controlled drug release system based on mesoporous silica nanoparticles was constructed for targeted drug delivery to tumor cells to reduce systemic toxicity and improve the therapeutic efficacy. METHODS: In this study, the doxorubicin (DOX) was filled into the mesopores of the functional MSNs (DMSNs)...
February 6, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28165548/enhanced-antitumor-efficacy-of-resveratrol-loaded-nanocapsules-in-colon-cancer-cells-physicochemical-and-biological-characterization
#15
M Feng, L-X Zhong, Z-Y Zhan, Z-H Huang, J-P Xiong
OBJECTIVE: The aim of present work was to prepare resveratrol-loaded lipid-core-nanocapsule (RSV-LNC) and to characterize its ability to target the colon cancer cells. MATERIALS AND METHODS: The lipid-core nanocapsule was prepared by precipitation method. The nanoparticle was prepared and evaluated regarding physical, chemical and biological parameters. RESULTS: The average size of optimized nanocapsule was ~159 nm with a uniform size distribution index of 0...
January 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28164203/modulating-motility-of-intracellular-vesicles-in-cortical-neurons-with-nanomagnetic-forces-on-chip
#16
Anja Kunze, Coleman Tylor Murray, Chanya Godzich, Jonathan Lin, Keegan Owsley, Andy Tay, Dino Di Carlo
Vesicle transport is a major underlying mechanism of cell communication. Inhibiting vesicle transport in brain cells results in blockage of neuronal signals, even in intact neuronal networks. Modulating intracellular vesicle transport can have a huge impact on the development of new neurotherapeutic concepts, but only if we can specifically interfere with intracellular transport patterns. Here, we propose to modulate motion of intracellular lipid vesicles in rat cortical neurons based on exogenously bioconjugated and cell internalized superparamagnetic iron oxide nanoparticles (SPIONs) within microengineered magnetic gradients on-chip...
February 6, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28160452/nanoescapology-progress-toward-understanding-the-endosomal-escape-of-polymeric-nanoparticles
#17
REVIEW
Laura I Selby, Christina M Cortez-Jugo, Georgina K Such, Angus P R Johnston
Using nanoparticles to deliver drugs to cells has the potential to revolutionize the treatment of many diseases, including HIV, cancer, and diabetes. One of the major challenges facing this field is controlling where the drug is trafficked once the nanoparticle is taken up into the cell. In particular, if drugs remain localized in an endosomal or lysosomal compartment, the therapeutic can be rendered completely ineffective. To ensure the design of more effective delivery systems we must first develop a better understanding of how nanoparticles and their cargo are trafficked inside cells...
February 3, 2017: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
https://www.readbyqxmd.com/read/28159697/engineered-nanoparticles-of-titanium-dioxide-tio2-uptake-and-biological-effects-in-a-sea-bass-cell-line
#18
S Picchietti, C Bernini, V Stocchi, A R Taddei, R Meschini, A M Fausto, L Rocco, F Buonocore, D Cervia, G Scapigliati
With the rapid development of nanotechnology there has been a corresponding increase in the application of titanium dioxide nanoparticles (TiO2-NPs) in various consumer and industrial products, consequently their potential health hazards and environmental effects are considered an aspect of great concern. In the present study, in order to assess the impact of TiO2-NPs in the marine environment, the biological effects of TiO2-NPs on a sea bass cell line (DLEC) were investigated. Cells were exposed for 24 h to different concentrations of TiO2-NPs (1, 8, 40, 200 and 1000 μg/ml) or co-exposed with CdCl2 (Cd)...
January 31, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28155940/synergistic-chemotherapeutic-effect-of-sorafenib-loaded-pullulan-dox-conjugate-nanoparticles-against-murine-breast-carcinoma
#19
Junhui Sui, Yani Cui, Hanxu Cai, Shaoquan Bian, Zhiyi Xu, Ling Zhou, Yong Sun, Jie Liang, Yujiang Fan, Xingdong Zhang
pH-Sensitive pullulan-doxorubicin conjugates encapsulating sorafenib (P-Dox/S) nanoparticles were developed as a synergistic combinatorial delivery system against murine breast carcinoma. The nanoparticles can encapsulate Dox and sorafenib with ultra-high loading capacity (65.34 wt%) through chemical conjugation and physical loading, whereas can remain stable under physiological conditions and gradually release Dox and sorafenib with the decreasing pH. These conjugates can be effectively internalized and clearly suppress 4T1 cell growth in vitro...
February 3, 2017: Nanoscale
https://www.readbyqxmd.com/read/28155880/self-assemble-nanoparticles-based-on-polypeptides-containing-c-terminal-luminescent-pt-cysteine-complex
#20
E G Vlakh, E V Grachova, D D Zhukovsky, A V Hubina, A S Mikhailova, J R Shakirova, V V Sharoyko, S P Tunik, T B Tennikova
The growing attention to the luminescent nanocarriers is strongly stimulated by their potential application as drug delivery systems and by the necessity to monitor their distribution in cells and tissues. In this communication we report on the synthesis of amphiphilic polypeptides bearing C-terminal phosphorescent label together with preparation of nanoparticles using the polypeptides obtained. The approach suggested is based on a unique and highly technological process where the new phosphorescent Pt-cysteine complex serves as initiator of the ring-opening polymerization of α-amino acid N-carboxyanhydrides to obtain the polypeptides bearing intact the platinum chromophore covalently bound to the polymer chain...
February 3, 2017: Scientific Reports
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