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cell internalization nanoparticles

Li Qun Jiang, Ting Yu Wang, Thomas J Webster, Hua-Jian Duan, Jing Ying Qiu, Zi Ming Zhao, Xiao Xing Yin, Chun Li Zheng
Biodegradable nanomaterials have been widely used in numerous medical fields. To further improve such efforts, this study focused on the intracellular disposition of chitosan nanoparticles (CsNPs) in macrophages, a primary cell of the mononuclear phagocyte system (MPS). Such interactions with the MPS determine the nanoparticle retention time in the body and consequently play a significant role in their own clinical safety. In this study, various dye-labeled CsNPs (about 250 nm) were prepared, and a murine macrophage cell line (RAW 264...
2017: International Journal of Nanomedicine
Dajana Lichtenstein, Thomas Meyer, Linda Böhmert, Sabine Juling, Christoph Fahrenson, Soeren Selve, Andreas F Thünemann, Jan Meijer, Irina Estrela-Lopis, Albert Braeuning, Alfonso Lampen
The elucidation of mechanisms underlying cellular uptake of nanoparticles is an important topic in nanotoxicological research. Most studies dealing with silver nanoparticle uptake provide only qualitative data about internalization efficiency and do not consider nanoparticle-specific dosimetry. Therefore, we performed a comprehensive comparison of the cellular uptake of differently coated silver nanoparticles of comparable size in different human intestinal Caco-2 cell-derived models to cover also the influence of the intestinal mucus barrier and uptake-specialized M-cells...
September 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
Zuoheng Zhang, Xubo Lin, Ning Gu
Plasma membrane internalization of nanoparticles (NPs) is important for their biomedical applications such as drug-delivery carriers. On one hand, in order to improve their half-life in circulation, PEGylation has been widely used. However, it may hinder the NPs' membrane internalization ability. On the other hand, higher temperature could enhance the membrane permeability and may affect the NPs' ability to enter into or exit from cells. To make full use of their advantages, we systematically investigated the effects of temperature and PEG density on the translocation of PEGylated nanoparticles across the plasma asymmetric membrane of eukaryotic cells, using near-atom level coarse-grained molecular dynamics simulations...
September 6, 2017: Colloids and Surfaces. B, Biointerfaces
Lindsey C Felix, Van A Ortega, Greg G Goss
The ever-growing production of engineered nanoparticles (NPs) for use in many agricultural, commercial, consumer, and industrial applications will lead to their accidental or intentional release into the environment. Potential routes of environmental exposure include manufacturing or transport spills, disposal of NP-containing products down the drain and/or in landfills, as well as direct usage on agricultural land. Therefore, NPs will inevitably contaminate aquatic environments and interact with resident organisms...
September 7, 2017: Aquatic Toxicology
B Sikora, P Kowalik, J Mikulski, K Fronc, I Kamińska, M Szewczyk, A Konopka, K Zajdel, R Minikayev, K Sobczak, W Zaleszczyk, A Borodziuk, J Rybusiński, J Szczytko, A Sienkiewicz, T Wojciechowski, P Stępień, M Frontczak-Baniewicz, M Łapiński, G Wilczyński, W Paszkowicz, A Twardowski, D Elbaum
Water-soluble upconversion nanoparticles (UCNPs), based on polyvinylpyrrolidone (PVP)-coated NaYF4:Er(3+),Yb(3+),Gd(3+), with various concentrations of Gd(3+) ions and relatively high upconversion efficiencies, were synthesized. The internalization and cytotoxicity of the thus obtained UCNPs were evaluated in three cell lines (HeLa, HEK293 and astrocytes). No cytotoxicity was observed even at concentrations of UCNPs up to 50 μg ml(-1). The fate of the UCNPs within the cells was studied by examining their upconversion emission spectra with confocal microscopy and confirming these observations with transmission electron microscopy...
September 15, 2017: Nanoscale
Cleofe Palocci, Alessio Valletta, Laura Chronopoulou, Livia Donati, Marco Bramosanti, Elisa Brasili, Barbara Baldan, Gabriella Pasqua
PLGA NPs' cell uptake involves different endocytic pathways. Clathrin-independent endocytosis is the main internalization route. The cell wall plays a more prominent role than the plasma membrane in NPs' size selection. In the last years, many studies on absorption and cell uptake of nanoparticles by plants have been conducted, but the understanding of the internalization mechanisms is still largely unknown. In this study, polydispersed and monodispersed poly(lactic-co-glycolic) acid nanoparticles (PLGA NPs) were synthesized, and a strategy combining the use of transmission electron microscopy (TEM), confocal analysis, fluorescently labeled PLGA NPs, a probe for endocytic vesicles (FM4-64), and endocytosis inhibitors (i...
September 14, 2017: Plant Cell Reports
Michael A Harris, Timothy R Pearce, Thomas Pengo, Huihui Kuang, Colleen Forster, Efrosini Kokkoli
In this work we hypothesized that the chemokine fractalkine can serve as a cancer molecular target. We engineered aptamer micelles functionalized with an outer poly(ethylene glycol) (PEG) corona, and investigated the extent and efficacy of using them as a targeting tool against fractalkine-expressing colon adenocarcinoma cells. In vitro cell binding results showed that aptamer micelles bound and internalized to fractalkine-expressing cancer cells with the majority of the micelles found free in the cytoplasm...
September 11, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
Dovile Baziulyte-Paulaviciene, Vitalijus Karabanovas, Marius Stasys, Greta Jarockyte, Vilius Poderys, Simas Sakirzanovas, Ricardas Rotomskis
Upconverting nanoparticles (UCNPs) are promising, new imaging probes capable of serving as multimodal contrast agents. In this study, monodisperse and ultrasmall core and core-shell UCNPs were synthesized via a thermal decomposition method. Furthermore, it was shown that the epitaxial growth of a NaGdF4 optical inert layer covering the NaGdF4:Yb,Er core effectively minimizes surface quenching due to the spatial isolation of the core from the surroundings. The mean diameter of the synthesized core and core-shell nanoparticles was ≈8 and ≈16 nm, respectively...
2017: Beilstein Journal of Nanotechnology
Katharina Herkendell, Ran Tel-Vered, Andreas Stemmer
Enzymatic fuel cells may become more accessible for applications powering portable electronic devices by broadening the range of potentially usable fuels and oxidizers. In this work we demonstrate the operation of an integrated, yet versatile multi-substrate biofuel cell utilizing either glucose, fructose, sucrose or combinations of thereof as biofuels, and molecular oxygen originating from solution phase and/or an internal chemical source, as the oxidizer. In order to achieve this goal we designed an enzymatic cascade-functionalized anode consisting of invertase (INV), mutarotase (MUT), glucose oxidase (GOX), and fructose dehydrogenase (FDH), deposited on top of a mesoporous carbon nanoparticle matrix, in which electron relay molecules had been entrapped...
September 13, 2017: Nanoscale
Manuela Gabriel, Jose Moya, Luciana Gallo, Fernando Marengo, Laura Estrada
Most accepted single particle tracking methods are able to obtain high-resolution trajectories for relatively short periods of time. In this work we apply a straightforward combination of single-particle tracking microscopy and metallic nanoparticles internalization on mouse chromaffin cells to unveil the intracellular trafficking mechanism of metallic-nanoparticle-loaded vesicle (MNP-V) complexes after clathrin dependent endocytosis. We found that directed transport is the major route of MNP-Vs intracellular trafficking after stimulation (92...
September 13, 2017: Methods and Applications in Fluorescence
Cem Varan, Erem Bilensoy
Background: Brain tumors are the most common tumors among adolescents. Although some chemotherapeutics are known to be effective against brain tumors based on cell culture studies, the same effect is not observed in clinical trials. For this reason, the development of drug delivery systems is important to treat brain tumors and prevent tumor recurrence. The aim of this study was to develop core-shell polymeric nanoparticles with positive charge by employing a chitosan coating. Additionally, an implantable formulation for the chemotherapeutic nanoparticles was developed as a bioadhesive film to be applied at the tumor site following surgical operation for brain glioma treatment...
2017: Beilstein Journal of Nanotechnology
Vicard Du, Nathalie Luciani, Sophie Richard, Gaëtan Mary, Cyprien Gay, François Mazuel, Myriam Reffay, Philippe Menasché, Onnik Agbulut, Claire Wilhelm
The ability to create a 3D tissue structure from individual cells and then to stimulate it at will is a major goal for both the biophysics and regenerative medicine communities. Here we show an integrated set of magnetic techniques that meet this challenge using embryonic stem cells (ESCs). We assessed the impact of magnetic nanoparticles internalization on ESCs viability, proliferation, pluripotency and differentiation profiles. We developed magnetic attractors capable of aggregating the cells remotely into a 3D embryoid body...
September 12, 2017: Nature Communications
Suhana Ahmad, Anes Ateqah Zamry, Hern-Tze Tina Tan, Kah Keng Wong, JitKang Lim, Rohimah Mohamud
Gold nanoparticles (NPs) have been proposed as a highly potential tool in immunotherapies due to its advantageous properties including customizable size and shapes, surface functionality and biocompatibility. Dendritic cells (DCs), the sentinels of immune response, have been of interest to be manipulated by using gold NPs for targeted delivery of immunotherapeutic agent. Researches done especially in human DCs showed a variation of gold NPs effects on cellular uptake and internalization, DC maturation and subsequent T cells priming as well as cytotoxicity...
September 9, 2017: Molecular Immunology
Marc Bachofner, Tobias Speicher, Roman L Bogorad, Sukalp Muzumdar, Carina P Derrer, Fabrizio Hürlimann, Friederike Böhm, Paolo Nanni, Tobias Kockmann, Ekaterina Kachaylo, Michael Meyer, Susagna Padrissa-Altés, Rolf Graf, Daniel G Anderson, Victor Koteliansky, Ulrich Auf dem Keller, Sabine Werner
The liver is the only organ in mammals that fully regenerates even after major injury. To identify orchestrators of this regenerative response, we performed quantitative large-scale proteomics analysis of cytoplasmic and nuclear fractions from normal versus regenerating mouse liver. Proteins of the ubiquitin-proteasome pathway were rapidly upregulated after two-third hepatectomy, with the ubiquitin ligase Nedd4-1 being a top hit. In vivo knockdown of Nedd4-1 in hepatocytes through nanoparticle-mediated delivery of small interfering RNA caused severe liver damage and inhibition of cell proliferation after hepatectomy, resulting in liver failure...
August 30, 2017: Developmental Cell
Marjan Ghorbani, Hamed Hamishehkar
The aim of this study was to design and develop a new pH-responsive nano-platform for controlled and targeted delivery of anticancer drugs. Engineering of pH-responsive nanocarriers was prepared via decoration of gold nanoparticles (NPs) by thiolated (methoxy-poly(ethylene glycol)-b-poly((2-dimethylamino) ethyl methacrylate-co-itaconic acid) (mPEG-b-p(DMAEMA-co-IA) copolymer and fully characterized by various techniques and subsequently used for loading and targeted delivery of anticancer agent, methotrexate (MTX)...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Yang Xia, Huimin Chen, Feimin Zhang, Chongyun Bao, Michael D Weir, Mark A Reynolds, Junqing Ma, Ning Gu, Hockin H K Xu
In this study, a novel calcium phosphate cement containing gold nanoparticles (GNP-CPC) was developed. Its osteogenic induction ability on human dental pulp stem cells (hDPSCs) was investigated for the first time. The incorporation of GNPs improved hDPSCs behavior on CPC, including better cell adhesion (about 2-fold increase in cell spreading) and proliferation, and enhanced osteogenic differentiation (about 2-3-fold increase at 14days). GNPs endow CPC with micro-nano-structure, thus improving surface properties for cell adhesion and subsequent behaviors...
September 5, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
Rui Sun, Ling Yin, Shaohua Zhang, Lei He, Xiaju Cheng, Anna Wang, Huawei Xia, Haibin Shi
A simple and mild light-triggered fluorescent labeling approach, based on the photolysis and reactive carbene insertion of diazirine, to rapidly generate fluorescent nanoparticles is reported. Proof of concept studies demonstrated that the non-fluorescent SiNPs could be covalently labeled by diazirine-conjugated fluorescein (dFITC) upon photo irradiation (λ=365 nm) to afford fluorescent SiNPs with good fluorescent stability and low cytotoxicity. In vitro cellular imaging results indicated that the internalization of SiNPs into living cells could be readily visualized based on fluorescein-labeled SiNPs (F-SiNPs) in real time...
September 8, 2017: Chemistry: a European Journal
Saheli Pradhan, Damodhara Rao Mailapalli
Nanoparticles with their unique surface properties can modulate the physiological, biochemical, and physicochemical pathways, such as photosynthesis, respiration, nitrogen metabolism, and solute transport. In this context, researchers have developed a wide range of engineered nanomaterials (ENMs) for the improvement of growth and productivity by modulating the metabolic pathways in plants. This class of tailor-made materials can potentially lead to the development of a new group of agrochemical nanofertilizers...
September 19, 2017: Journal of Agricultural and Food Chemistry
Lina Herrera Estrada, Huixia Wu, Kevin Ling, Guikai Zhang, Ronen Sumagin, Charles A Parkos, Rheinallt M Jones, Julie A Champion, Andrew S Neish
Bacterial enteric pathogens have evolved efficient mechanisms to suppress mammalian inflammatory and immunoregulatory pathways. By exploiting the evolutionary relationship between the gut and pathogenic bacteria, we have developed a potential mucosal therapeutic. Our findings suggest that engineered preparations of the Salmonella acetyltransferase, AvrA, suppress acute inflammatory responses such as those observed in inflammatory bowel disease (IBD). We created 125 nm diameter cross-linked protein nanoparticles directly from AvrA and carrier protein to deliver AvrA in the absence of Salmonella...
September 5, 2017: ACS Nano
Christina L Parker, Qi Yang, Bing Yang, Justin D McCallen, Steven I Park, Samuel K Lai
Pretargeting represents a promising strategy to enhance delivery of nanoparticles. The strategy involves first introducing bispecific antibodies or fusion proteins (BFP) that can bind specific epitopes on target cells, which in turn can capture subsequently administered effector molecules, such as radionuclides or drug-loaded nanoparticles. Nevertheless, it remains unclear whether BFP that bind slowly- or non-internalizing epitopes on target cells can facilitate efficient intracellular delivery. Here, we investigated the cellular uptake of biotin-functionalized nanoparticles with streptavidin-scFv against TAG-72, a membrane protein on Jurkat T-cell leukemia cell...
September 1, 2017: Acta Biomaterialia
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