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https://www.readbyqxmd.com/read/27911977/selective-inactivation-of-enzymes-conjugated-to-nanoparticles-using-tuned-laser-illumination
#1
Asaf Ilovitsh, Pazit Polak, Zeev Zalevsky, Orit Shefi
We report a novel method for specific deactivation of conjugated enzymes using laser-heated gold nanoparticles. Current methods involve treatment of the entire solution, thereby inactivating all bioactive components. Our method enables inactivation of only a single or subset of targeted enzymes. The selected enzyme is pre-conjugated to gold nanoparticles, which are specifically heated by a laser tuned to their surface plasmon resonance. We demonstrate inactivation of a selected enzyme, glucose oxidase, within a mixture of biomolecules...
December 2, 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27910990/a-ph-responsive-sequential-disassembly-nanohybrid-for-mitochondrial-targeting
#2
Lijia Li, Wei Sun, Lian Li, Yuanyuan Liu, Lei Wu, Fengling Wang, Zhou Zhou, Zhirong Zhang, Yuan Huang
Cationic materials have been reported as promising tools for targeting to mitochondria which are the "power houses" and "metabolic garbage keepers" of cells. However, their positive nature also restricts their in vivo application due to the quick clearance. Herein, we fabricated a nanohybrid consisting of the pH-responsive N-(2-hydroxypropyl)methacrylamide (HPMA) co-polymer (R-P) shells and positive mesoporous silica nanoparticle cores via electrostatic interaction. The anticancer drug, docetaxel (DTX), was encapsulated in the positive MSN cores (MSN-DTX)...
December 2, 2016: Nanoscale
https://www.readbyqxmd.com/read/27905610/dendritic-polyglycerol-and-n-isopropylacrylamide-based-thermoresponsive-nanogels-as-smart-carriers-for-controlled-delivery-of-drugs-through-the-hair-follicle
#3
Fitsum Feleke Sahle, Michael Giulbudagian, Julian Bergueiro, Jürgen Lademann, Marcelo Calderón
Nanoparticles with a size of several hundred nanometers can effectively penetrate into the hair follicles and may serve as depots for controlled drug delivery. However, they can neither overcome the hair follicle barrier to reach the viable cells nor release the loaded drug adequately. On the other hand, small drug molecules cannot penetrate deep into the hair follicles. Thus, the most efficient way for drug delivery through the follicular route is to employ nanoparticles that can release the drug close to the target structure upon exposure to some external or internal stimuli...
December 1, 2016: Nanoscale
https://www.readbyqxmd.com/read/27899122/endocytosed-nanoparticles-hold-endosomes-and-stimulate-binucleated-cells-formation
#4
Lin Xia, Weihong Gu, Mingyi Zhang, Ya-Nan Chang, Kui Chen, Xue Bai, Lai Yu, Juan Li, Shan Li, Gengmei Xing
BACKGROUND: Nanotechnology developed rapidly in cellular diagnosis and treatment, the endocytic system was an important pathway for targeting cell. In the research of developing macrophages as drug carriers or important therapeutic targets, an interesting phenomenon, internalized nanoparticles induced to form binucleated macrophages, was found although the particles dose did not cause obvious cytotoxicity. RESULTS: Under 25 μg/ml, internalized 30 nm polystyrene beads(30 nm Ps nanoparticles) induced the formation of binucleated macrophages when they entered into endosomes via the endocytic pathway...
November 29, 2016: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/27895482/nanoparticle-based-strategy-for-personalized-b-cell-lymphoma-therapy
#5
Nicola M Martucci, Nunzia Migliaccio, Immacolata Ruggiero, Francesco Albano, Gaetano Calì, Simona Romano, Monica Terracciano, Ilaria Rea, Paolo Arcari, Annalisa Lamberti
B-cell lymphoma is associated with incomplete response to treatment, and the development of effective strategies targeting this disease remains challenging. A new personalized B-cell lymphoma therapy, based on a site-specific receptor-mediated drug delivery system, was developed in this study. Specifically, natural silica-based nanoparticles (diatomite) were modified to actively target the antiapoptotic factor B-cell lymphoma/leukemia 2 (Bcl2) with small interfering RNA (siRNA). An idiotype-specific peptide (Id-peptide) specifically recognized by the hypervariable region of surface immunoglobulin B-cell receptor was exploited as a homing device to ensure specific targeting of lymphoma cells...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27892533/small-sized-stable-lipid-nanoparticle-for-the-efficient-delivery-of-sirna-to-human-immune-cell-lines
#6
Takashi Nakamura, Moeka Kuroi, Yuki Fujiwara, Shota Warashina, Yusuke Sato, Hideyoshi Harashima
Gene silencing by small interfering RNA (siRNA) is useful for analyzing the functions of human immune cells. However, the transfection of siRNA to human immune cells is difficult. Here, we used a multifunctional envelope-type nanodevice (MEND) containing YSK12-C4 (YSK12-MEND) to efficiently introduce siRNA to human immune cell lines, Jurkat, THP-1, KG-1 and NK92. The YSK12-MEND was transfected to human immune cell lines at a siRNA dose range of 1-30 nM, resulting that maximum gene silencing efficiencies at the mRNA level in Jurkat, THP-1, KG-1 and NK92 were 96%, 96%, 91% and 75%, respectively...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27890657/targeting-tumor-highly-expressed-lat1-transporter-with-amino-acid-modified-nanoparticles-toward-a-novel-active-targeting-strategy-in-breast-cancer-therapy
#7
Lin Li, Xingsheng Di, Mingrui Wu, Zhisu Sun, Lu Zhong, Yongjun Wang, Qiang Fu, Qiming Kan, Jin Sun, Zhonggui He
Designing active targeting nanocarriers with increased cellular accumulation of chemotherapeutic agents is a promising strategy in cancer therapy. Herein, we report a novel active targeting strategy based on the large amino acid transporter 1 (LAT1) overexpressed in a variety of cancers. Glutamate was conjugated to polyoxyethylene stearate as a targeting ligand to achieve LAT1-targeting PLGA nanoparticles. The targeting efficiency of nanoparticles was investigated in HeLa and MCF-7 cells. Significant increase in cellular uptake and cytotoxicity was observed in LAT1-targeting nanoparticles compared to the unmodified ones...
November 25, 2016: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/27889952/effects-of-different-doses-of-2-3-dimercaptosuccinic-acid-modified-fe2-o3-nanoparticles-on-intercalated-discs-in-engineered-cardiac-tissues
#8
Yongchao Mou, Shuanghong Lv, Fei Xiong, Yao Han, Yuwei Zhao, Junjie Li, Ning Gu, Jin Zhou
Although iron oxide nanoparticles (IRONs) were applied in clinical magnetic resonance imaging in vivo and magnetic tissue engineering in vitro widely, the underlying effects of IRONs on the development of cardiomyocytes especially the intercellular junctions, intercalated discs (IDs), remain an unknown issue. Given the critical role of three-dimensional (3D) engineered cardiac tissues (ECTs) in evaluation of nanoparticles toxicology, it remained necessary to understand the effects of IRONs on IDs assembly of cardiomyocytes in 3D environment...
November 27, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27888498/chitosan-nanolayered-cisplatin-loaded-lipid-nanoparticles-for-enhanced-anticancer-efficacy-in-cervical-cancer
#9
Jing-Yi Wang, Yu Wang, Xia Meng
In this study, cisplatin (CDDP)-loaded chitosan-coated solid lipid nanoparticles (SLN) was successfully formulated to treat HeLa cervical carcinoma. The formulation nanoparticles were nanosized and exhibited a controlled release of drug in physiological conditions. The blank nanoparticles exhibited an excellent biocompatibility profile indicating its suitability for cancer targeting. The incorporation of CDDP in SLN remarkably increased the cancer cell death as evident from the MTT assay. Importantly, CDDP-loaded chitosan-coated SLN (CChSLN) significantly (P < 0...
December 2016: Nanoscale Research Letters
https://www.readbyqxmd.com/read/27886401/not-all-that-glitters-is-gold-electron-microscopy-study-on-uptake-of-gold-nanoparticles-in-daphnia-magna-and-related-artefacts
#10
Louise Helene Søgaard Jensen, Lars Michael Skjolding, Amalie Thit, Sara Nørgaard Sørensen, Carsten Købler, Kristian Mølhave, Anders Baun
Increasing use of engineered nanoparticles has led to extensive research into their potential hazards to the environment and human health. Cellular uptake from the gut is sparsely investigated and microscopy techniques applied for uptake studies can result in misinterpretations. Various microscopy techniques are used to investigate internalization of 10 nm gold nanoparticles in Daphnia magna gut lumen and gut epithelial cells upon 24h exposure and outline potential artefacts, i.e. high contract precipitates from sample preparation related to these techniques...
November 25, 2016: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/27885823/novel-functionalization-strategies-of-polymeric-nanoparticles-as-carriers-for-brain-medications
#11
Corinne Portioli, Michele Bovi, Donatella Benati, Marta Donini, Massimiliano Perduca, Alessandro Romeo, Stefano Dusi, Hugo L Monaco, Marina Bentivoglio
For targeted brain delivery, nanoparticles (NPs) should bypass the blood-brain barrier (BBB). Novel functionalization strategies, based on low-density lipoprotein receptor (LDLR) binding domain, have been here tested to increase the brain targeting efficacy of poly d,l-lactic-co-glycolic acid (PLGA) NPs, biodegradable and suited for biomedical applications. Custom-made PLGA NPs were functionalized with an apolipoprotein E modified peptide (pep-apoE) responsible for LDLR binding, or with lipocalin-type prostaglandin-d-synthase (L-PGDS), highly expressed in the brain...
November 5, 2016: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/27883931/disassembly-of-amphiphilic-small-molecular-prodrug-with-fluorescence-switch-induced-by-ph-and-folic-acid-receptors-for-targeted-delivery-and-controlled-release
#12
Zhigang Xu, Xiaoxiao Shi, Meili Hou, Peng Xue, Yong-E Gao, Shiying Liu, Yuejun Kang
We develop a new type of pH-responsive amphiphilic small molecular prodrug by conjugating folic acid with anti-tumour doxorubicin via a hydrazone bond. This prodrug is featured by high and precise drug loading (55.4wt%), which can self-assemble into micellar nanoparticles in neutral environment while disassemble in the presence of tumour cells expressing folic acid receptors or the acidic tumoral endosomal environment. The prodrug nanoparticles can effectively improve anticancer efficacy due to the features of pH-triggered drug release and targeted delivery...
November 17, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27882809/mechanisms-of-cell-uptake-inflammatory-potential-and-protein-corona-effects-with-gold-nanoparticles
#13
Yang Li, Nancy A Monteiro-Riviere
AIM: To assess inflammation, cellular uptake and endocytic mechanisms of gold nanoparticles (AuNP) in human epidermal keratinocytes with and without a protein corona. MATERIALS & METHODS: Human epidermal keratinocytes were exposed to 40 and 80 nm AuNP with lipoic acid, polyethylene glycol (PEG) and branched polyethyleneimine (BPEI) coatings with and without a protein corona up to 48 h. Inhibitors were selected to characterize endocytosis. RESULTS & CONCLUSION: BPEI-AuNP showed the greatest uptake, while PEG-AuNP had the least...
November 24, 2016: Nanomedicine
https://www.readbyqxmd.com/read/27881802/elongated-magnetite-nanoparticle-formation-from-a-solid-ferrous-precursor-in-a-magnetotactic-bacterium
#14
Jens Baumgartner, Nicolas Menguy, Teresa Perez Gonzalez, Guillaume Morin, Marc Widdrat, Damien Faivre
Magnetotactic bacteria are aquatic microorganisms that intracellularly mineralize ferrimagnetic nanoparticles enabling the cells to align with the geomagnetic field. The bacteria produce a magnetic mineral of species-specific phase (magnetite Fe(II)Fe(III)2O4 or greigite Fe(II)Fe(III)2S4), size, morphology and particle assembly. Several species produce crystals of unusual elongated particle shapes, which break the symmetry of the thermodynamically favoured isometric morphology. Such morphologies are thought to affect domain size and orientation of the internal magnetization...
November 2016: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/27877873/preparation-of-magnetic-mesoporous-silica-nanoparticles-as-a-multifunctional-platform-for-potential-drug-delivery-and-hyperthermia
#15
Xia Yu, Yufang Zhu
We report the preparation of magnetic mesoporous silica (MMS) nanoparticles with the potential multifunctionality of drug delivery and magnetic hyperthermia. Carbon-encapsulated magnetic colloidal nanoparticles (MCN@C) were used to coat mesoporous silica shells for the formation of the core-shell structured MMS nanoparticles (MCN@C/mSiO2), and the rattle-type structured MMS nanoparticles (MCN/mSiO2) were obtained after the removal of the carbon layers from MCN@C/mSiO2 nanoparticles. The morphology, structure, magnetic hyperthermia ability, drug release behavior, in vitro cytotoxicity and cellular uptake of MMS nanoparticles were investigated...
2016: Science and Technology of Advanced Materials
https://www.readbyqxmd.com/read/27874198/crataeva-nurvala-nanoparticles-inhibit-virulence-factors-and-biofilm-formation-in-clinical-isolates-of-pseudomonas-aeruginosa
#16
Syed Ghazanfar Ali, Mohammad Azam Ansari, Haris M Khan, Mohammad Jalal, Abbas Ali Mahdi, Swaranjit Singh Cameotra
Green synthesized nanoparticles have gained great attention due to their non-toxic and non-hazardous nature. In the present study, bark extract of the medicinal plant in Ayurveda Crataeva nurvala (Buch-Ham) (CN) was chosen for the biosynthesis of silver nanoparticles (AgNPs). These NPs were characterized by Ultra violet visible spectroscopy, Fourier Transform Infra Red, Atomic Force Microscopy, and Transmission Electron Microscopy (TEM). The average particle size of green synthesized CN-AgNPs was 15.2 ± 1...
November 22, 2016: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/27872483/targeted-delivery-system-for-cancer-cells-consist-of-multiple-ligands-conjugated-genetically-modified-ccmv-capsid-on-doxorubicin-gnps-complex
#17
Indu Barwal, Rajiv Kumar, Suneel Kateriya, Amit Kumar Dinda, Subhash Chandra Yadav
Targeted nano-delivery vehicles were developed from genetically modified Cowpea chlorotic mottle virus (CCMV) capsid by ligands bioconjugation for efficient drug delivery in cancer cells. RNA binding (N 1-25aa) and β-hexamer forming (N 27-41aa) domain of capsid was selectively deleted by genetic engineering to achieve the efficient in vitro assembly without natural cargo. Two variants of capsids were generated by truncating 41 and 26 amino acid from N terminus (NΔ41 and NΔ26) designated as F1 and F2 respectively...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27867056/manipulation-of-ph-sensitive-interactions-between-podophyllotoxin-chitosan-for-enhanced-controlled-drug-release
#18
Chao Chen, Yong Yu, Xiaoli Wang, Ping Shi, Yibing Wang, Ping Wang
Podophyllotoxin (PPT) offers a broad-spectrum of anticancer activities, but little has been reported for its controlled release. This work shows that by manipulating molecular interactions between PPT and Chitosan, efficient nanoscale capsulation of PPT can be realized. The drug encapsulation efficiency is as high as 52%, with a final particle drug loading in the order of 10% (wt/wt). It further demonstrates that changes in pH can also significantly affect the rate of drug release from the Chitosan nanoparticles...
November 17, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27866417/bodipy-conjugated-chitosan-nanoparticles-as-a-fluorescent-probe
#19
Gizem Bor, Muhammed Üçüncü, Mustafa Emrullahoğlu, Aysel Tomak, Gülşah Şanlı-Mohamed
Recently, development of fluorescent nanoparticle-based probes for various bioimaging applications has attracted great attention. This work aims to develop a new type fluorescent nanoparticle conjugate and evaluate its cytotoxic effects on A549 and BEAS 2B cell lines. Throughout the study, ionically crosslinked chitosan nanoparticles (CNs) were conjugated with carboxylated 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY-COOH). The results of conjugates (BODIPY-CNs) were investigated with regard to their physic-chemical, optical, cytotoxic properties and cellular internalization...
November 21, 2016: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/27864056/lactoferrin-nanoparticle-mediated-targeted-delivery-of-5-fluorouracil-for-enhanced-therapeutic-efficacy
#20
Sonali Kumari, Anand K Kondapi
Malignant melanoma is an aggressive form of skin cancer with high mortality rates. Common treatments for malignant melanoma involve a combination of radiotherapy and chemotherapy with fluorouracil (5-FU). A major challenge with melanoma treatment is active resistance to chemotherapeutic drugs. Superior treatment outcome lies on balance involving optimum therapeutic doses and the side effects associated with dose escalation. The study aimed to efficiently entrap 5-FU in lactoferrin nanoparticles (LfNPs) in an attempt to enhance its therapeutic efficacy...
November 15, 2016: International Journal of Biological Macromolecules
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